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Arbuscular mycorrhizal fungus strain and application thereof to resisting root-knot nematode

A technology of arbuscular mycorrhizal fungi and root-knot nematodes, applied in the fields of application, fungi, nematicides, etc., can solve the problems of reducing farmers' income, not allowed to use, high toxicity, etc.

Active Publication Date: 2010-05-12
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of controlling root-knot nematode is to adopt chemical methods, but most chemical nematicides have high toxicity, and are not allowed to be used in the production of pollution-free vegetables.
The occurrence of vegetable root-knot nematode seriously restricts vegetable production and reduces farmers' income

Method used

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  • Arbuscular mycorrhizal fungus strain and application thereof to resisting root-knot nematode
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  • Arbuscular mycorrhizal fungus strain and application thereof to resisting root-knot nematode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1. Isolation and identification of arbuscular mycorrhizal fungus strain Glomus mosei 96022

[0014] Isolation of strains: In September 2006, rhizosphere soil of pepper was collected from Yunnan, and strains were isolated from the collected soil.

[0015] The separation method is as follows: Weigh 50 grams of capsicum rhizosphere soil, put it into a large beaker, add 1000ml of water, stir, pass through a double-layer sampling sieve after standing for 10 seconds (upper sieve 20 mesh, lower sieve 300 mesh), and collect 300ml of water with water. The residue on the mesh sieve was placed in a petri dish, and a single spore of the strain was picked under a stereomicroscope, inoculated in a mixture of river sand and zeolite with a particle size of 1-2mm and equal volumes, and sorghum was planted, and three cultures were cultivated. After 1 month, the zeolite-sand mixture containing the spores of the strain, infected root segments and extraroot mycelium was harvested. ...

Embodiment 2

[0019] Example 2, identification of the resistance of the arbuscular mycorrhizal fungus strain Glomus mosei 96022 to root-knot nematodes

[0020] 1 Materials and methods

[0021] 1.1 Test time and place

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Abstract

The invention discloses an arbuscular mycorrhizal fungus strain and application thereof to resisting root-knot nematode. The strain is arbuscular mycorrhizal fungus strain Glomus mosseae 96022CGMCC No.3451. The arbuscular mycorrhizal fungus strain Glomus mosseae 96022 can be applied to preventing and curing diseases caused by plant root-knot nematode. Proved by pot experiment results, the inoculation of the arbuscular mycorrhizal fungus strain Glomus mosseae can obviously inhibit damages to cucumber roots of the root-knot nematode so as to have a certain preventing and curing effect, and the preventing and curing efficiency reaches 41 percent.

Description

technical field [0001] The invention relates to an arbuscular mycorrhizal fungus strain and its application in resisting root-knot nematodes. Background technique [0002] Root-knot nematode (Meloidogyne) is one of the most harmful plant pathogenic nematodes. It has many types, wide distribution and strong pathogenicity. Its compound infection with other pathogenic bacteria will cause more damage to crops. The most important types of pathogenic nematodes in vegetable production in my country are: Meloidogyne incognita, M.hapla, M.Javanica, and M.arenaria. Among them, M.incognita The area of ​​occurrence is relatively large, accounting for 77% of the total occurrence, and the types of harmful vegetables are the most, and it has become the dominant population of harmful vegetables. Especially in recent years, due to the large-scale planting of vegetables in protected areas in northern my country, more suitable conditions have been provided for the overwintering of root-knot ne...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 王幼珊张淑彬邹国元王美菊
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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