1507 results about "Rhizosphere" patented technology

Disclosed herein are methods, compositions, and kits for determining the presence or absence of a target polypeptide (e.g., a polypeptide involved in nitrogen fixation) in a sample (e.g., a sample of soil from the rhizosphere of a non-leguminous plant).

The invention relates to the technical field of microorganisms, and discloses burkholderia gladioli as well as a bacterial agent and application of the burkholderia gladioli. The Burkholderia gladioli (Burkholderia gladioli) CCTCC (China Center For Type Culture Collection) NO: M20242288 provided by the invention has good capacities of fixing nitrogen, dissolving phosphorus, dissolving zinc, producing siderophores and oxidizing sulfur, and can be used for regulating the nutrient structure of soil and converting substances which are difficult to utilize by plants in the soil, so that the growth of the plants is promoted, and the stress resistance of the plants is improved; the strain also can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Fusarium verticillium and Rosellinia necatrix, can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium and Rosellinia necatrix, and can also be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix. Besides, the burkholderia gladioli provided by the invention is a strain separated from rape farmland soil, and compared with a strain screened by extreme environment and laboratory mutagenesis, the burkholderia gladioli has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the burkholderia gladioli stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect. Wide application prospects are realized.

The invention relates to the technical field of plant growth-promoting bacterium screening, in particular to a thunberg fritillary bulb growth-promoting bacterium colony screening method and system based on image recognition. The screening method comprises the following steps: establishing a three-dimensional gradient dilution system of a thunberg fritillary bulb rhizosphere soil sample, and collecting a bacterial colony original image under a specific wavelength combination by adopting a multispectral imaging device. According to the method, by establishing a multispectral collaborative imaging mechanism and a dynamic growth modeling system, the technical problems of feature dimension deficiency and space-time correlation fracture in a traditional method are effectively solved. An improved wavelet transform algorithm is adopted to break through the optical interference limitation of an agar matrix, and high-fidelity extraction of bacterial colony intrinsic textures is realized; according to the constructed three-dimensional feature space fusion model, morphological topological parameters, texture entropy distribution and growth differential features are deeply coupled through nonlinear mapping, and the biological correlation of feature representation is remarkably improved.

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

The invention relates to the technical field of intelligent control, in particular to a monopotassium phosphate intelligent control method and system.The method comprises the following steps that rhizosphere phosphorus and potassium concentration changes are obtained, whether the rhizosphere phosphorus and potassium concentration changes are in a critical rate interval or not is judged, an effective absorption time period identifier is generated, and an organ amplification ratio is analyzed to judge a cooperation period; and extracting a stalk concentration ratio, comparing the stalk concentration ratio with a reference interval to generate an application inhibition identifier, fusing information to judge a fertilization window, adjusting the concentration according to the root hair amplification and the water consumption rate, and generating a control instruction set. According to the method, the effective time period of phosphorus and potassium nutrient absorption of the crops is determined through crop rhizosphere phosphorus and potassium ion concentration difference calculation and average treatment, the objective interval of crop nutrient absorption rate indexes is defined, blind fertilization is avoided, the pertinence and accuracy of nutrient absorption and utilization of the crops are enhanced, the excessive application state of nutrient elements is dynamically identified, and the crop quality is improved. The risk of excessive nutrients caused by nutrient enrichment of crops is prevented, and resource waste and environmental burden are effectively reduced.

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

The invention relates to the technical field of microorganisms, in particular to application of Cladosporium LC3 in crop growth promotion and bacterial wilt resistance, the strain LC3 belongs to Cladosporium and Cladosporium immonda species, the strain is separated from Guangxi sugarcane rhizosphere soil, experimental evaluation screening finds that the strain has a good growth promotion function on tomatoes and fresh ginger, and the strain can be applied to crop growth promotion and bacterial wilt resistance. The yield of ginger is remarkably increased, and the growth vigor of tomato plants is effectively improved; meanwhile, the strain has a good prevention and treatment effect on tomato and ginger bacterial wilt and has good plant bacterial wilt resistance, and screening verification of the strain provides a new fungicide and a raw material for later-period growth of an anti-microbial agent or a biological organic fertilizer.

The invention discloses application of sodium alginate-carboxymethyl chitosan composite hydrogel in preparation of a biological bacterial fertilizer, the sodium alginate-carboxymethyl chitosan composite hydrogel is used for preparation of the biological bacterial fertilizer, and is further used for preparation of the biological bacterial fertilizer with plant growth-promoting rhizobacteria as an effective component, and the sodium alginate-carboxymethyl chitosan composite hydrogel can be stored for a long time and has a good application prospect. The growth promoting effect is stably exerted. The plant growth-promoting bacteria are effectively wrapped and efficiently delivered through a nanometer means to enhance the growth-promoting effect on crops, the adaptability of the plant growth-promoting bacteria to the acid environment is effectively improved, and the advantage of the plant growth-promoting bacteria for promoting plant growth in acid soil is expanded. The biological bacterial fertilizer is suitable for neutral and acidic environments, and the effects of the biological bacterial fertilizer in the aspects of soil remediation and improvement, crop quality and efficiency improvement, crop stress resistance improvement and the like are fully exerted, so that the purposes of losing weight and improving efficiency are achieved.

The invention provides an ionic type rare earth tailing restoration method, and relates to the technical field of environment restoration. Through soil improvement, local dominant plant planting and rhizosphere growth-promoting bacteria reinforcement, the heavy metal content, acidity and soil fertility of the rare earth tailing area are improved, soil with reduced heavy metal content, reduced acidity and increased fertility is obtained, continuous growth of arbor-shrub-grass three-layer dominant plants on the rare earth tailing area is promoted, and the yield of the arbor-shrub-grass three-layer dominant plants is increased. And finally, effective repair of the rare earth tailings is realized.

The invention belongs to the technical field of agricultural Internet of Things, and discloses an Internet of Things-based intelligent agricultural cloud platform data acquisition and management system, which comprises the following steps of: acquiring plant rhizosphere microenvironment information, generating rhizosphere original data and performing correlation analysis to obtain rhizosphere electrochemical interaction data; collecting canopy microclimate information, and performing temperature and humidity gradient quantification processing and pore dynamic response characteristic extraction to obtain pore response characteristic data; collecting field illumination information, and carrying out multispectral distribution analysis and photoperiod sensitivity differentiation analysis to obtain a crop photoperiod sensitivity spectrum; performing multi-dimensional association fusion to generate crop physiological status comprehensive evaluation data; agricultural measure analysis and resource allocation optimization are carried out, and finally a resource allocation instruction is generated and agricultural operation scheduling is carried out; according to the invention, intelligent acquisition, analysis and management of data in each link of agricultural production are realized, and the utilization efficiency of agricultural resources and the production accuracy are improved.

The invention relates to the technical field of agricultural environment control, in particular to an automatic environment regulation and control system for planting traditional Chinese medicinal materials, which comprises an environment quality evaluation module, an environment regulation and control demand analysis module, a planting mode optimization module and an intelligent regulation and control module. According to the method, environmental changes can be comprehensively understood by comprehensively evaluating the environmental quality, monitoring and analyzing the volatility of environmental data and judging the actual influence on the root growth of the traditional Chinese medicinal materials, a more stable and suitable growth condition is provided for the traditional Chinese medicinal materials, and fine adjustment of the environmental data is realized by simulating multi-environment combination, so that the accuracy of the traditional Chinese medicinal materials is improved. The method not only optimizes the rhizosphere environment, but also effectively promotes the absorption of the traditional Chinese medicinal materials to the target nutrient elements, enhances the growth efficiency and quality of the crops, dynamically regulates and controls the environmental parameters, further improves the nutrient absorption capacity of the traditional Chinese medicinal materials, ensures the health of the root system and the overall development of the crops, and improves the economic benefits of the crops. And synchronous improvement of production efficiency and crop quality is ensured.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention relates to a Chinese wildrye rhizosphere growth-promoting bacterium and application of the Chinese wildrye rhizosphere growth-promoting bacterium in growth promotion and drought resistance of Chinese wildrye, and belongs to the technical field of microorganisms, the strain is classified and named as Paraburkholderia graminis, the strain number is B24, and the preservation number is CGMCC No.31907. The strain provided by the invention is derived from the intergrassland soil of leymus chinensis in an excessive grazing grassland, has good growth promoting characteristics of producing IAA, producing ACC deaminase, fixing nitrogen, dissolving phosphorus and the like, and also can obviously enhance the drought resistance of the leymus chinensis. When the strain is inoculated to the rhizosphere of a leymus chinensis seedling, the growth of the leymus chinensis can be obviously promoted, and the overground and underground biomass of the leymus chinensis is increased; the activity of antioxidant enzymes is reduced and the hormone content in leymus chinensis leaves is changed, so that drought stress is relieved, the photosynthesis of leymus chinensis is enhanced, and the drought resistance of leymus chinensis is remarkably improved. Therefore, the Burkholderia parakholderia can be used for development of a microbial fertilizer for grassland plants and applied to a drought stress habitat, so that the drought resistance of the grassland plant leymus chinensis is improved, and grassland management is optimized.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention belongs to the technical field of microorganisms, and provides a growth-promoting P.adami M6 and application thereof, the P.adami M6 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242835, the preservation date is December 18, 2024, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, and the preservation number is CCTCC No: M20242835. The strain is separated from rhizosphere soil of field planting foxtail millet in the Inner Mongolia red peak area, and is obtained through artificial separation and purification; the strain has the growth promoting characteristics of nitrogen fixation, phosphorus dissolution, IAA production and siderophore production; the M6 fungicide is used for inoculating millet, so that the growth of millet seedlings can be obviously promoted, and the plant height, the root length and the overground part and underground part biomass of the millet are obviously increased; m6 is used as a microbial agent to soak seeds during sowing and irrigate a bacterial liquid once 7 days after sowing, so that the growth effect of millet is best.

The invention discloses a rhizosphere deposited carbon flux real-time monitoring method adopting a sensor array, and relates to the technical field of soil carbon cycle monitoring, and the method comprises the steps: determining a layered sampling depth and a horizontal concentric circle partition range based on the three-dimensional distribution characteristics of a target plant root system, and mapping the layered sampling depth and the horizontal concentric circle partition range to a preset three-dimensional grid coordinate system; gas sensors and environmental parameter sensors are deployed according to root system partitions, and a multi-dimensional sensor array is constructed. Through the three-dimensional grid coordinate system and the root system density gradient model, accurate spatial mapping of rhizosphere carbon flux is realized, high matching of sensor layout and root system biomass distribution is ensured by utilizing division of vertical layering and horizontal concentric circle partition, and the gas sensor and the environmental parameter sensor are cooperatively deployed, so that the reliability of the system is improved. The device can capture instantaneous changes such as root exudate pulse and microbial respiration, generates a continuous and consistent carbon flux data set, and provides a high-resolution dynamic observation basis for researching plant-soil-microbial interaction.

The invention relates to the field of intelligent irrigation, and discloses an intelligent irrigation system and method for a crop root system, and the system comprises a root zone sensing module which is used for collecting soil environment parameters through multi-mode soil sensor arrays disposed at soil layers of different depths, generating a sensing matrix, and transmitting the sensing matrix to a root zone sensing module; the sensing matrix is used for representing water potential, pH value, nutrient concentration and temperature information in each soil layer; and the root system density analysis module is connected with the root zone sensing module, and is used for constructing a root system density weight matrix based on root system space distribution information obtained by underground computed tomography, and multiplying the sensing matrix by the root system density weight matrix element by element to generate a root zone state vector. Accurate generation of regulation and control signals is achieved, the micro-fluidic execution module can adjust the irrigation flow and the fertilization proportion in real time according to the actual requirements of the root zone, and therefore it is guaranteed that the rhizosphere environment is always close to the optimal physiological interval.

The invention discloses a poplar degenerated forest stand accurate restoration method, and relates to the technical field of poplar degenerated forest stand restoration, and the method comprises the steps: obtaining a multi-temporal satellite remote sensing image and an unmanned plane multi-spectral image of a target area; in combination with soil moisture content, rhizosphere respiration rate and micrometeorological data collected by ground Internet of Things sensing nodes arranged in a forest stand, geographic coordinate registration and time sequence alignment are performed on the data to form a multi-source observation data set; the method comprises the following steps: extracting vegetation indexes, short-wave infrared reflectivity, soil moisture and rhizosphere physiological parameters based on a multi-source observation data set, constructing a degradation diagnosis index fusing spectral change, moisture stress and root activity, calculating the degradation degree of each pixel, and generating a degradation level spatial distribution diagram; the area with the degradation degree reaching a set threshold value is determined as a to-be-repaired area; and selecting representative poplar individuals in the to-be-repaired area, measuring the photosynthetic ability of the individuals, and calculating the individual competition intensity in combination with the three-dimensional point cloud data.

The invention relates to the technical field of agricultural irrigation, in particular to an intelligent biological irrigation system and method based on remote control, and the system comprises a biological sensing unit, a neural computing decision engine, a driving execution unit, and a communication optimization architecture. Wherein the biological sensing unit is used for monitoring multi-modal data of plant physiological status, rhizosphere microenvironment and meteorological parameters in real time; the neural computing decision engine is used for constructing a pulse neural network, fusing a plant dimensional management system signal transduction mechanism and a multi-agent optimization algorithm, and generating a millisecond-level dynamic irrigation strategy; the driving execution unit is used for millimeter-level water quantity adjustment and pipe network self-repairing of the irrigation execution unit; the communication optimization architecture is used for seamless coverage and secure transmission of irrigation data, and meanwhile, a strategy is optimized by applying algorithms such as reinforcement learning and the like. Therefore, the problems of water resource waste, poor irrigation effect, untimely irrigation strategy adjustment and the like in the prior art are solved.

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention provides a composite microbial agent for decomposing insoluble phosphorus in soil and application thereof, and relates to the technical field of microorganisms, the active ingredients of the composite microbial agent comprise Achromobacter xylosoxidans, Burkholderia cepacia and Burkholderia parafungi, the Achromobacter xylosoxidans, the Burkholderia cepacia and the Burkholderia parafungi all have dissolving capacity for Ca3 (PO4) 2, FePO4, AlPO4, lecithin and calcium phytate, and the composite microbial agent can be used for decomposing insoluble phosphorus in soil. And the five insoluble phosphorus sources can be synergistically dissolved. The compound microbial agent is subjected to shake culture, the phosphate-solubilizing activity can reach 53.32 mg.L <-1 >, the phosphate-solubilizing function is stable, meanwhile, the compound microbial agent has the characteristics of degrading lignin and generating siderophore and indoleacetic acid, and the compound microbial agent has remarkable phosphate-solubilizing and growth-promoting effects when being applied to the vicinity of plant rhizosphere soil; the technical problem that in the prior art, the variety of phosphorus dissolving sources of phosphate solubilizing bacteria is single or the stability of the phosphate solubilizing function is poor is solved.

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention relates to the technical field of soil remediation, and discloses a method for synergistically remediating organic contaminated soil by combining microorganisms with plant root systems, which comprises the following steps: carrying out physicochemical property detection and condition adjustment on the contaminated soil in a target remediation area; applying a composition containing functional microorganisms and a controllable release activation component to the contaminated soil; plants with rhizosphere secretion ability are planted in the restoration area; plant root exudates are used for inducing the composition to release active components, so that microorganisms are activated, and organic pollutants are jointly degraded; and maintaining the repair cycle, and performing process regulation and control until the repair is completed. A double-layer microcapsule structure is constructed, the outer layer adopts chitosan and an induction material, and the outer layer can swell or disintegrate under the action of plant rhizosphere secretions, so that responsive release of active components of the inner layer is achieved, and the effect of improving release accuracy and microbial activation efficiency is achieved.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of microbial agents, in particular to a composite microbial agent and application of the composite microbial agent in prevention and treatment of forest root diseases, and the composite microbial agent comprises the following components in parts by weight: 15-25 parts of Trichoderma harzianum ATCC 20847; 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 606 and 10 to 20 parts of bacillus subtilis ATCC 607. 5-10 parts of a seaweed extract; 3 to 8 parts of chitosan; 2-5 parts of humic acid; 1 to 3 parts of glycine betaine; 0.5 to 1.5 parts of a trace element compound; 30 to 40 parts of perlite powder; according to the present invention, the fertilizer has characteristics of significant inhibition effect on a variety of forest root pathogenic bacteria, wide prevention and control spectrum, and long-term and stable disease prevention effect by adjusting the rhizosphere micro-ecosystem. Diseases are prevented and controlled, and the growth and root development of the forest trees are remarkably promoted.

The invention discloses an intelligent precise irrigation and nutrient regulation and control integrated system and method for rice, and particularly relates to the technical field of intelligent agriculture, and the system comprises a multi-mode sensing module, an edge computing node, an intelligent decision module, a water and fertilizer cooperative execution module and a credible monitoring module. Rice rhizosphere and leaf environment data are collected through an underground nano sensor and a canopy multispectral imaging device, data fusion and model reasoning are carried out through edge nodes, an irrigation strategy value is output in combination with a mechanism model and an AI model, and a pulse injection device is controlled to achieve precise water and fertilizer integrated operation. The system supports network disconnection operation and night model updating, and the stability and the self-adaptive capability are improved. According to the method, the environment data acquisition precision and the irrigation decision response speed are improved, the interpretability and robustness of the model are enhanced, the water resource utilization efficiency and the intelligent management level are effectively improved, and the method has wide application and popularization values.

The invention discloses a regulation and control method and system for rice production, relates to the technical field of agricultural production and intelligent control, is used for solving the problems of low production efficiency, uneven crop growth and unstable quality, and calculates a light-temperature matching coefficient through real-time monitoring of a root activity index and a leaf surface transpiration amount in combination with illumination intensity and canopy temperature difference. The nutrient absorption capacity of the rice is evaluated by detecting the rhizosphere conductivity change and the soil nutrient content, when the rice growth state is poor and the resource regulation capacity is high, an enhanced regulation and control mechanism is automatically entered, and regulation and control increasing levels are set according to the current rice canopy carbon dioxide exchange rate and the current air flow disturbance index; the resource supply coefficient is calculated and the regulation rate is determined through regulation and control level increase, so that the resource supply is optimized, and the agricultural production efficiency and the resource utilization rate are improved.

The invention belongs to the technical field of rhizosphere microorganisms, and provides a strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens and application of the strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens in growth promoting and repairing of saline-alkali soil. The strain not only grows fast, but also has growth promoting functions such as nitrogen fixation, phosphorus solubilization and potassium solubilization. The strain is preserved in the China General Microbiological Culture Collection Center on March 11, 2025, called CGMCC for short, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the biological preservation number of the strain is CGMCC NO.33793. The SP-38 strain has multiple growth promoting functions, and through salt stress water culture tests and in-situ saline-alkali soil pot tests of corn, the strain has good effects in the aspects of improving the crop yield and repairing saline-alkali soil.