1292 results about "Rhizosphere" patented technology

The invention relates to the technical field of plant growth-promoting bacterium screening, in particular to a thunberg fritillary bulb growth-promoting bacterium colony screening method and system based on image recognition. The screening method comprises the following steps: establishing a three-dimensional gradient dilution system of a thunberg fritillary bulb rhizosphere soil sample, and collecting a bacterial colony original image under a specific wavelength combination by adopting a multispectral imaging device. According to the method, by establishing a multispectral collaborative imaging mechanism and a dynamic growth modeling system, the technical problems of feature dimension deficiency and space-time correlation fracture in a traditional method are effectively solved. An improved wavelet transform algorithm is adopted to break through the optical interference limitation of an agar matrix, and high-fidelity extraction of bacterial colony intrinsic textures is realized; according to the constructed three-dimensional feature space fusion model, morphological topological parameters, texture entropy distribution and growth differential features are deeply coupled through nonlinear mapping, and the biological correlation of feature representation is remarkably improved.

The invention relates to the technical field of intelligent control, in particular to a monopotassium phosphate intelligent control method and system.The method comprises the following steps that rhizosphere phosphorus and potassium concentration changes are obtained, whether the rhizosphere phosphorus and potassium concentration changes are in a critical rate interval or not is judged, an effective absorption time period identifier is generated, and an organ amplification ratio is analyzed to judge a cooperation period; and extracting a stalk concentration ratio, comparing the stalk concentration ratio with a reference interval to generate an application inhibition identifier, fusing information to judge a fertilization window, adjusting the concentration according to the root hair amplification and the water consumption rate, and generating a control instruction set. According to the method, the effective time period of phosphorus and potassium nutrient absorption of the crops is determined through crop rhizosphere phosphorus and potassium ion concentration difference calculation and average treatment, the objective interval of crop nutrient absorption rate indexes is defined, blind fertilization is avoided, the pertinence and accuracy of nutrient absorption and utilization of the crops are enhanced, the excessive application state of nutrient elements is dynamically identified, and the crop quality is improved. The risk of excessive nutrients caused by nutrient enrichment of crops is prevented, and resource waste and environmental burden are effectively reduced.

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

The invention belongs to the technical field of agricultural Internet of Things, and discloses an Internet of Things-based intelligent agricultural cloud platform data acquisition and management system, which comprises the following steps of: acquiring plant rhizosphere microenvironment information, generating rhizosphere original data and performing correlation analysis to obtain rhizosphere electrochemical interaction data; collecting canopy microclimate information, and performing temperature and humidity gradient quantification processing and pore dynamic response characteristic extraction to obtain pore response characteristic data; collecting field illumination information, and carrying out multispectral distribution analysis and photoperiod sensitivity differentiation analysis to obtain a crop photoperiod sensitivity spectrum; performing multi-dimensional association fusion to generate crop physiological status comprehensive evaluation data; agricultural measure analysis and resource allocation optimization are carried out, and finally a resource allocation instruction is generated and agricultural operation scheduling is carried out; according to the invention, intelligent acquisition, analysis and management of data in each link of agricultural production are realized, and the utilization efficiency of agricultural resources and the production accuracy are improved.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention belongs to the technical field of microorganisms, and provides a growth-promoting P.adami M6 and application thereof, the P.adami M6 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242835, the preservation date is December 18, 2024, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, the preservation number is CCTCC No: M20242835, and the preservation number is CCTCC No: M20242835. The strain is separated from rhizosphere soil of field planting foxtail millet in the Inner Mongolia red peak area, and is obtained through artificial separation and purification; the strain has the growth promoting characteristics of nitrogen fixation, phosphorus dissolution, IAA production and siderophore production; the M6 fungicide is used for inoculating millet, so that the growth of millet seedlings can be obviously promoted, and the plant height, the root length and the overground part and underground part biomass of the millet are obviously increased; m6 is used as a microbial agent to soak seeds during sowing and irrigate a bacterial liquid once 7 days after sowing, so that the growth effect of millet is best.

The invention discloses a rhizosphere deposited carbon flux real-time monitoring method adopting a sensor array, and relates to the technical field of soil carbon cycle monitoring, and the method comprises the steps: determining a layered sampling depth and a horizontal concentric circle partition range based on the three-dimensional distribution characteristics of a target plant root system, and mapping the layered sampling depth and the horizontal concentric circle partition range to a preset three-dimensional grid coordinate system; gas sensors and environmental parameter sensors are deployed according to root system partitions, and a multi-dimensional sensor array is constructed. Through the three-dimensional grid coordinate system and the root system density gradient model, accurate spatial mapping of rhizosphere carbon flux is realized, high matching of sensor layout and root system biomass distribution is ensured by utilizing division of vertical layering and horizontal concentric circle partition, and the gas sensor and the environmental parameter sensor are cooperatively deployed, so that the reliability of the system is improved. The device can capture instantaneous changes such as root exudate pulse and microbial respiration, generates a continuous and consistent carbon flux data set, and provides a high-resolution dynamic observation basis for researching plant-soil-microbial interaction.

The invention relates to the field of intelligent irrigation, and discloses an intelligent irrigation system and method for a crop root system, and the system comprises a root zone sensing module which is used for collecting soil environment parameters through multi-mode soil sensor arrays disposed at soil layers of different depths, generating a sensing matrix, and transmitting the sensing matrix to a root zone sensing module; the sensing matrix is used for representing water potential, pH value, nutrient concentration and temperature information in each soil layer; and the root system density analysis module is connected with the root zone sensing module, and is used for constructing a root system density weight matrix based on root system space distribution information obtained by underground computed tomography, and multiplying the sensing matrix by the root system density weight matrix element by element to generate a root zone state vector. Accurate generation of regulation and control signals is achieved, the micro-fluidic execution module can adjust the irrigation flow and the fertilization proportion in real time according to the actual requirements of the root zone, and therefore it is guaranteed that the rhizosphere environment is always close to the optimal physiological interval.

The invention discloses a poplar degenerated forest stand accurate restoration method, and relates to the technical field of poplar degenerated forest stand restoration, and the method comprises the steps: obtaining a multi-temporal satellite remote sensing image and an unmanned plane multi-spectral image of a target area; in combination with soil moisture content, rhizosphere respiration rate and micrometeorological data collected by ground Internet of Things sensing nodes arranged in a forest stand, geographic coordinate registration and time sequence alignment are performed on the data to form a multi-source observation data set; the method comprises the following steps: extracting vegetation indexes, short-wave infrared reflectivity, soil moisture and rhizosphere physiological parameters based on a multi-source observation data set, constructing a degradation diagnosis index fusing spectral change, moisture stress and root activity, calculating the degradation degree of each pixel, and generating a degradation level spatial distribution diagram; the area with the degradation degree reaching a set threshold value is determined as a to-be-repaired area; and selecting representative poplar individuals in the to-be-repaired area, measuring the photosynthetic ability of the individuals, and calculating the individual competition intensity in combination with the three-dimensional point cloud data.

The invention relates to the technical field of agricultural irrigation, in particular to an intelligent biological irrigation system and method based on remote control, and the system comprises a biological sensing unit, a neural computing decision engine, a driving execution unit, and a communication optimization architecture. Wherein the biological sensing unit is used for monitoring multi-modal data of plant physiological status, rhizosphere microenvironment and meteorological parameters in real time; the neural computing decision engine is used for constructing a pulse neural network, fusing a plant dimensional management system signal transduction mechanism and a multi-agent optimization algorithm, and generating a millisecond-level dynamic irrigation strategy; the driving execution unit is used for millimeter-level water quantity adjustment and pipe network self-repairing of the irrigation execution unit; the communication optimization architecture is used for seamless coverage and secure transmission of irrigation data, and meanwhile, a strategy is optimized by applying algorithms such as reinforcement learning and the like. Therefore, the problems of water resource waste, poor irrigation effect, untimely irrigation strategy adjustment and the like in the prior art are solved.

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention relates to the technical field of soil remediation, and discloses a method for synergistically remediating organic contaminated soil by combining microorganisms with plant root systems, which comprises the following steps: carrying out physicochemical property detection and condition adjustment on the contaminated soil in a target remediation area; applying a composition containing functional microorganisms and a controllable release activation component to the contaminated soil; plants with rhizosphere secretion ability are planted in the restoration area; plant root exudates are used for inducing the composition to release active components, so that microorganisms are activated, and organic pollutants are jointly degraded; and maintaining the repair cycle, and performing process regulation and control until the repair is completed. A double-layer microcapsule structure is constructed, the outer layer adopts chitosan and an induction material, and the outer layer can swell or disintegrate under the action of plant rhizosphere secretions, so that responsive release of active components of the inner layer is achieved, and the effect of improving release accuracy and microbial activation efficiency is achieved.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of microbial agents, in particular to a composite microbial agent and application of the composite microbial agent in prevention and treatment of forest root diseases, and the composite microbial agent comprises the following components in parts by weight: 15-25 parts of Trichoderma harzianum ATCC 20847; 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 605, 10 to 20 parts of bacillus subtilis ATCC 606 and 10 to 20 parts of bacillus subtilis ATCC 607. 5-10 parts of a seaweed extract; 3 to 8 parts of chitosan; 2-5 parts of humic acid; 1 to 3 parts of glycine betaine; 0.5 to 1.5 parts of a trace element compound; 30 to 40 parts of perlite powder; according to the present invention, the fertilizer has characteristics of significant inhibition effect on a variety of forest root pathogenic bacteria, wide prevention and control spectrum, and long-term and stable disease prevention effect by adjusting the rhizosphere micro-ecosystem. Diseases are prevented and controlled, and the growth and root development of the forest trees are remarkably promoted.

The invention discloses an intelligent precise irrigation and nutrient regulation and control integrated system and method for rice, and particularly relates to the technical field of intelligent agriculture, and the system comprises a multi-mode sensing module, an edge computing node, an intelligent decision module, a water and fertilizer cooperative execution module and a credible monitoring module. Rice rhizosphere and leaf environment data are collected through an underground nano sensor and a canopy multispectral imaging device, data fusion and model reasoning are carried out through edge nodes, an irrigation strategy value is output in combination with a mechanism model and an AI model, and a pulse injection device is controlled to achieve precise water and fertilizer integrated operation. The system supports network disconnection operation and night model updating, and the stability and the self-adaptive capability are improved. According to the method, the environment data acquisition precision and the irrigation decision response speed are improved, the interpretability and robustness of the model are enhanced, the water resource utilization efficiency and the intelligent management level are effectively improved, and the method has wide application and popularization values.

The invention discloses a regulation and control method and system for rice production, relates to the technical field of agricultural production and intelligent control, is used for solving the problems of low production efficiency, uneven crop growth and unstable quality, and calculates a light-temperature matching coefficient through real-time monitoring of a root activity index and a leaf surface transpiration amount in combination with illumination intensity and canopy temperature difference. The nutrient absorption capacity of the rice is evaluated by detecting the rhizosphere conductivity change and the soil nutrient content, when the rice growth state is poor and the resource regulation capacity is high, an enhanced regulation and control mechanism is automatically entered, and regulation and control increasing levels are set according to the current rice canopy carbon dioxide exchange rate and the current air flow disturbance index; the resource supply coefficient is calculated and the regulation rate is determined through regulation and control level increase, so that the resource supply is optimized, and the agricultural production efficiency and the resource utilization rate are improved.

The invention belongs to the technical field of rhizosphere microorganisms, and provides a strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens and application of the strain of saline-alkali tolerant rhizosphere growth promoting serratia marcescens in growth promoting and repairing of saline-alkali soil. The strain not only grows fast, but also has growth promoting functions such as nitrogen fixation, phosphorus solubilization and potassium solubilization. The strain is preserved in the China General Microbiological Culture Collection Center on March 11, 2025, called CGMCC for short, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the biological preservation number of the strain is CGMCC NO.33793. The SP-38 strain has multiple growth promoting functions, and through salt stress water culture tests and in-situ saline-alkali soil pot tests of corn, the strain has good effects in the aspects of improving the crop yield and repairing saline-alkali soil.

The invention discloses a construction method of a soil microorganism nitrogen metabolism function prediction model, and relates to the technical field of environmental microbiology and agricultural information, and the method comprises the following steps: S1, constructing a spatial-temporal heterogeneity representation engine, and dynamically decoupling a multi-level gradient of a soil environment through an adaptive spatial-temporal mesh generation algorithm; according to the construction method of the soil microorganism nitrogen metabolism function prediction model, the adaptive capacity of the soil microorganism nitrogen metabolism function prediction model in a complex environment is improved, and multi-level environment gradient dynamic analysis from a centimeter-level rhizosphere microdomain to a kilometer-level landscape scale is realized; the problem of cross-scale feature fusion caused by temporal-spatial resolution mismatch of a traditional model is solved, the simplified hypothesis of a traditional statistical model on the synergistic-antagonistic relationship of microbial functional genes is broken through, a dynamic coupling mechanism of a nitrogen metabolism path under environmental disturbance is accurately quantified, and the prediction error is reduced compared with the prior art.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

Provided are a quality-improving and nitrogen-fixing coupled microbial agent and the use thereof. The quality-improving and nitrogen-fixing coupled microbial agent is a microbial composition, has coupled functions of improving quality and fixing nitrogen so as to improve the quality of products of leguminous crops, and has the functions of regulating and increasing the rhizobial abundance for the rhizosphere of leguminous crops and increasing the amount of nodules of leguminous crops. The microbial agent contains all gene sequences of nucleotide sequences shown as SEQ ID NO.1-12 or four or more gene sequences among same. When being applied to production of leguminous crops, such as soybeans, peanuts, peas, broad beans and cowpeas, the quality-improving and nitrogen-fixing coupled microbial agent has coupled functions of improving quality and fixing nitrogen and achieves quality improvement and nitrogen fixation and green yield increase. The microbial agent is easy to use, achieves remarkable social and ecological benefits, and facilitates popularization and use.

The invention relates to the technical field of fertilizers, in particular to an environment-friendly water-soluble fertilizer containing a seaweed extract and polyglutamic acid. The water-soluble fertilizer is prepared by preparing seaweed oligosaccharide and double-grafted poly-L-gamma-sodium glutamate and compounding and complexing the seaweed oligosaccharide and the double-grafted poly-L-gamma-sodium glutamate, has the characteristics of low salting-out, high stability, no precipitation, good slow release performance and the like, and can continuously release nutrients and keep a solution clear and transparent under a low-salt condition; and because the polyglutamic acid and seaweed extract compound drip irrigation agent has the chelation and water retention characteristics of the polyglutamic acid and the natural growth promoting effect of the seaweed extract, the crop rhizosphere nutrient absorption efficiency and stress resistance are remarkably improved, the compound drip irrigation agent is suitable for drip irrigation and water and fertilizer integrated application of greenhouse vegetables, fruit trees and field crops, and the comprehensive effects of high efficiency, low salt load and environment friendliness are achieved.

The invention provides an organic carrier in-root-rhizosphere growth-promoting microbial agent for ecological restoration of degraded grassland as well as a preparation method and application of the microbial agent, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth-promoting microbial agent disclosed by the invention is prepared from four kinds of growth-promoting bacteria (PGPE) in roots, four kinds of growth-promoting bacteria (PGPR) in roots and an organic carrier, the PGPE comprises terracotta warrior bacillus K4, bacillus 9-3-1, bacillus deserticola 11-5-4 and halomonas L31, the PGPR comprises serratia rubra 5, serratia rubra 23, cosinia cosinii 11 and enterobacter parabarium 24, and the organic carrier is a carbon-based organic fertilizer, a cattle and sheep manure organic fertilizer or biochar. When the organic carrier growth-promoting microbial agent is applied to the degraded grassland, the biomass and coverage of plant communities can be remarkably improved. The organic carrier growth-promoting microbial agent can be used for ecological restoration of degraded grassland.

The invention discloses a pseudomonas for antimony detoxification and autotrophic nitrogen fixation and application of the pseudomonas. The strain is separated from the rhizosphere of a pioneer plant in antimony-polluted soil of a tin mine in cold water river city, Hunan province, and is identified to be capable of quickly oxidizing high-toxicity antimony (III) into low-toxicity antimony (V) through autotrophic metabolism in a high-antimony environment, so that antimony detoxification is realized, and the detoxification rate is 108.6 (mu mol / (L.day)). The strain has autotrophic nitrogen fixation activity and can achieve autotrophic growth under the oligotrophic condition of a mining area, and the nitrogenase activity is larger than or equal to 223.4 (nmol C2H2 / (g.h)). Particularly, the antimony detoxification and nitrogen fixation capabilities of the strain have a synergistic effect, so that the strain has unique advantages in the field of heavy metal antimony pollution remediation, and dual effects of antimony pollution treatment and soil improvement can be synchronously realized; the development and application prospects are good.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention discloses a growth regulator for promoting colonization of plant rhizosphere microorganisms and application of the growth regulator to crops, a preparation method of the growth regulator comprises the following steps: S1, dropwise adding an aqueous solution of modified chitosan and a compound bacteria solution into a saturated boric acid solution of 1-2% calcium chloride, crosslinking and curing for 3-4 hours, washing with sterile water for 3-5 times, and drying to obtain the growth regulator for promoting colonization of the plant rhizosphere microorganisms; immobilized microorganisms are obtained; s2, sequentially adding nanocellulose crystals, sodium polyaspartate and organic fertilizer into the enteromorpha extracting solution, uniformly stirring and mixing, adding immobilized microorganisms, uniformly mixing, granulating, coating with modified chitosan, and drying to obtain the growth regulator. Through step-by-step design of microorganism immobilization, nutrition compounding and chitosan coating, function integration of microorganism protection, nutrition release and environmental response is achieved, the colonization efficiency of rhizosphere microorganisms is remarkably improved, the soil microenvironment is improved, and finally comprehensive improvement of plant growth and stress resistance is achieved; and the method has relatively high social use value and application prospect.

The invention discloses streptomyces sp. SK-2 and an application thereof in prevention and treatment of white cone rot. The streptomyces sp. SK-2 is separated from rhizosphere soil of cedarwood soil, the biocontrol bacterium can remarkably inhibit the occurrence area of the white cone rot disease under the indoor condition, the prevention effect reaches 68% or above, and potential development and application value is achieved.

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.