634 results about "Rhizosphere" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention discloses a poplar degenerated forest stand accurate restoration method, and relates to the technical field of poplar degenerated forest stand restoration, and the method comprises the steps: obtaining a multi-temporal satellite remote sensing image and an unmanned plane multi-spectral image of a target area; in combination with soil moisture content, rhizosphere respiration rate and micrometeorological data collected by ground Internet of Things sensing nodes arranged in a forest stand, geographic coordinate registration and time sequence alignment are performed on the data to form a multi-source observation data set; the method comprises the following steps: extracting vegetation indexes, short-wave infrared reflectivity, soil moisture and rhizosphere physiological parameters based on a multi-source observation data set, constructing a degradation diagnosis index fusing spectral change, moisture stress and root activity, calculating the degradation degree of each pixel, and generating a degradation level spatial distribution diagram; the area with the degradation degree reaching a set threshold value is determined as a to-be-repaired area; and selecting representative poplar individuals in the to-be-repaired area, measuring the photosynthetic ability of the individuals, and calculating the individual competition intensity in combination with the three-dimensional point cloud data.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention relates to the technical field of fertilizers, in particular to an environment-friendly water-soluble fertilizer containing a seaweed extract and polyglutamic acid. The water-soluble fertilizer is prepared by preparing seaweed oligosaccharide and double-grafted poly-L-gamma-sodium glutamate and compounding and complexing the seaweed oligosaccharide and the double-grafted poly-L-gamma-sodium glutamate, has the characteristics of low salting-out, high stability, no precipitation, good slow release performance and the like, and can continuously release nutrients and keep a solution clear and transparent under a low-salt condition; and because the polyglutamic acid and seaweed extract compound drip irrigation agent has the chelation and water retention characteristics of the polyglutamic acid and the natural growth promoting effect of the seaweed extract, the crop rhizosphere nutrient absorption efficiency and stress resistance are remarkably improved, the compound drip irrigation agent is suitable for drip irrigation and water and fertilizer integrated application of greenhouse vegetables, fruit trees and field crops, and the comprehensive effects of high efficiency, low salt load and environment friendliness are achieved.

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.

The invention provides a degradable sodded strip for ecological restoration and a preparation method thereof, and belongs to the technical field of sodded strips, the degradable sodded strip comprises an upper-layer fiber web, a middle-layer seed and nutrient layer and a lower-layer grid which are sequentially laminated and compounded from top to bottom; the upper-layer fiber web is made of a hydrophilic degradable material and is used for absorbing moisture and preserving soil moisture; the middle seed and nutrient layer comprises plant seeds and a nutrient controlled release unit; the lower-layer grid is a three-dimensional grid which is made of a degradable high polymer material, has a three-dimensional communicated micropore structure and is used for mechanically guiding root growth; the nutrient controlled release unit is a microcapsule which takes a fertilizer as a core material and takes an environment response type polymer as a wall material, and the microcapsule is anchored in the fiber interweaving nodes of the lower layer grid through a cross-linking agent. According to the invention, environmental response type precise controlled release of plant rhizosphere nutrients and three-dimensional mechanical guidance of a root system growth path can be realized, and the utilization rate of the fertilizer and the survival rate and long-term stability of vegetation recovery under poor site conditions such as side slopes, river banks and mines are effectively improved.

The invention belongs to the technical field of crop pollution prevention, and particularly relates to a rice heavy metal adsorption and isolation method based on iron agent regulation and control. According to the method, the iron agent is applied to the polluted rice field soil to induce the soil-rhizosphere interface to form an iron film, heavy metals (such as As, Cu, Pb and Cd) are fixed by virtue of adsorption and complexing effects of the iron agent, and migration of the heavy metals to rice roots is reduced, so that accumulation of the heavy metals at edible parts (leaves and ears) of the rice is reduced; the tolerance of the rice in a polluted environment is enhanced; and the risk of heavy metals in a food chain is reduced. Experiments show; through treatment of an iron film formed by a certain iron-based concentration, accumulation of the heavy metals in the rice leaves can be remarkably reduced, and the accumulation amount of the heavy metals in the rice leaves is far lower than the accumulation amount (1 / 40-1 / 120) of the heavy metals in rice roots. The invention provides an effective solution for farmland soil remediation and crop safety production.

The invention relates to the technical field of heavy metal contaminated soil remediation, in particular to a plant-microorganism-passivator synergistic remediation method for a heavy metal contaminated farmland, which comprises the following steps: step 1, constructing a soil digital twinborn model; step 2, intelligent decision making and verification of a collaborative restoration scheme; 3, soil pretreatment before remediation; step 4, hierarchical collaborative restoration and dynamic regulation and control; 5, performing long-term verification on the repair effect and performing model iteration; physical heat treatment is used as strengthening pretreatment, the occurrence form of heavy metal is changed through high temperature, biomass charcoal is generated in situ, passivation immobilization-microbial strengthening-plant extraction three-stage collaborative operation is carried out according to the time sequence, pollutants are rapidly locked through a passivating agent, rhizosphere immobilization is strengthened through functional microorganisms, plant growth is promoted, and the quality of plants is improved. And the hyperaccumulator finally removes the heavy metals from the soil, so that the unification of short-term control and long-term reduction is realized.

The invention relates to the technical field of image enhancement, in particular to a rhizosphere microflora distribution analysis method based on image enhancement, which comprises the following steps: acquiring a continuous microscopic image sequence of a rhizosphere microenvironment, converting the continuous microscopic image sequence into a gray data space, and constructing a standard input frame set for optical flow calculation. According to the method, the stability score of each feature is calculated by using the signal-to-noise ratio stability measurement function, and the long-life-cycle feature is screened and reserved, so that the random imaging noise of the short life cycle can be filtered fundamentally, and the topological structure of the target community is obtained; and mapping the topological structure back to an original image space by using an inverse persistence mapping algorithm to be fused with a continuous microscopic image sequence, and finally, remarkably enhancing weak biological signals while keeping original form details, thereby realizing high signal-to-noise ratio analysis of microflora spatial and temporal distribution in a complex rhizosphere environment.

The invention discloses a greenhouse tomato water-saving and quality-improving planting method with cooperation of shallow-buried drip irrigation and biochar, and the method comprises the following steps: detecting greenhouse tomato planting soil, and conditioning the soil texture according to a detection result; laying a drip irrigation system on the conditioned soil, and laying a soil monitoring network; based on the established dropper system and the soil detection network, tomato seedlings are planted, and initial moisture management is carried out on the tomato seedlings after planting; after the tomatoes survive after field planting, performing staged irrigation and water and fertilizer integrated management according to the requirements of the tomatoes in different growth stages; performing charcoal rhizosphere activation and function regulation on the tomatoes in the growth period; real-time data of a greenhouse environment and a soil monitoring network are integrated, an intelligent irrigation decision model is established and operated, and a dynamic optimization irrigation scheme is implemented; and carrying out oxidation and ecological improvement on the soil after tomato harvesting. The tomato planting quality can be improved, and the soil health condition can be improved.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a complex microbial inoculant containing streptomyces elongisporus and trichoderma Guizhou, the streptomyces elongisporus is streptomyces elongisporus A25, and the trichoderma Guizhou is trichoderma Guizhou NJAU4742. The invention further discloses application of the complex microbial inoculant to promotion of cucumber growth and application to promotion of seedless litchi yield improvement and fruit quality improvement. Streptomyces elongisporus A25 and trichoderma Guizhou NJAU4742 which are separated and screened from seedless litchi tree rhizosphere soil are prepared into the composite microbial agent, the composite microbial agent is applied to a cucumber pot experiment, cucumber growth can be remarkably promoted, and when the composite microbial agent is applied to a seedless litchi field experiment, seedless litchi yield improvement and fruit quality improvement can be remarkably promoted. The complex microbial inoculant disclosed by the invention has important significance on sustainable development of the seedless litchi industry.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention relates to the technical field of seed coating materials, and discloses a wheat and rice seed coating material and a coating process. The wheat and rice seed coating material comprises micron iron powder, nano calcium phosphate, nano magnesium hydroxide and a binder, the addition amounts of the micron iron powder, the nano calcium phosphate and the nano magnesium hydroxide respectively account for 10%-20%, 2%-10% and 3%-10% of the total weight of the seeds; the binder is at least one of a polyvinyl alcohol colloidal solution, a carboxymethyl cellulose colloidal solution and a hydroxypropyl methyl cellulose colloidal solution. The invention also provides a coating process of the wheat and rice seed coating material. The coating material disclosed by the invention not only can protect seeds, but also can efficiently adsorb and fix heavy metal ions of lead, cadmium and arsenic after the micron iron powder, the nano calcium phosphate and the nano magnesium hydroxide are slowly released in soil, so that integrated prevention and control from'seeds' to'rhizosphere 'is realized.

The invention discloses a growth-promoting halophilic Clariella sackii BWT 1.2507 strain and a preparation method of the growth-promoting halophilic Clariella sackii BWT 1.2507 strain. The growth-promoting halophilic Clariella sackii BWT 1.2507 strain has the advantages that the growth- The invention relates to a bacillus subtilis BWT1.2507, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on September 28, 2025, the address of the GDMCC is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong province, the postcode is 510070, and the preservation number is GDMCC No: 67048. The halophilic Sacchaffia halmapaa BWT1.2507 is separated and screened from the corn rhizosphere saline-alkali soil, has the functional characteristics of saline-alkali resistance growth, nitrogen fixation, organic phosphorus dissolution, IAA secretion and siderophore production, has obvious application effects in promotion of healthy growth of corn plants and alkali and salt reduction of saline-alkali soil, can be developed as an agricultural microbial agent, and has broad application prospects. The application potential in improvement of saline-alkali soil is huge.

The invention discloses a composite stable moringa oleifera polysaccharide cadmium-resistant preparation as well as a preparation method and application thereof, and relates to the technical field of cadmium-resistant preparations. The cadmium blocking preparation is composed of moringa oleifera polysaccharide, a compound stabilizer and a phosphate buffer solution, the compound stabilizer is composed of sodium alginate and nano silicon dioxide according to the mass ratio of 1: (1.0-1.5), and the pH value of the preparation is 6.0-7.0. The moringa oleifera polysaccharide is applied to cadmium prevention and control of Chinese cabbages in alkaline cadmium-polluted soil (pH 6.5-7.5), and accurate regulation and control are achieved in a rhizosphere irrigation mode. The sodium alginate-nano silicon dioxide composite stabilizer system synergistically exerts the dual functions of dispersion stability and slow release protection, and ensures that the moringa oleifera polysaccharide keeps activity in the soil environment. The preparation has the advantages of being small in dosage, low in cost, easy and convenient to operate, environmentally friendly and the like, a scientific and feasible technical scheme is provided for safe utilization of cadmium-polluted farmland in the alkaline soil area, and the preparation has important application value and popularization prospects.

The invention discloses a planting method for intercropping flue-cured tobacco and chive and application of the planting method in relieving root rot of a tobacco field. Belongs to the technical field of agricultural planting and comprises the steps of transplanting flue-cured tobaccos in mid-to-late April, transplanting chive seedlings to two sides of tobacco ridges within 7 days after tobacco plants are transplanted, regularly harvesting chives, harvesting tobacco leaves on the upper portions of the tobacco plants in September, pulling out tobacco stems in late October, and immediately preparing soil and sowing green manure; the green manure plants are ploughed in the soil to be decomposed, flue-cured tobacco is planted in April, and the steps are repeated in sequence. The method is easy to operate, the microenvironment of organic carbon, enzyme activity and microorganisms of rhizosphere soil of tobacco plants in the tobacco field can be improved, the multiple cropping index of the soil in the tobacco field is increased, meanwhile, soil-borne diseases caused by long-term single cropping of the flue-cured tobaccos in the tobacco field can be inhibited, and effective technical guarantee is provided for sustainable production of the flue-cured tobaccos.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The invention discloses a biological agent for preventing and treating panax notoginseng root rot, and relates to the technical field of biological prevention and treatment, the biological agent is composed of four layers of functional florae: an A layer of sentinel-decoding flora is used for identifying a pathogen quorum sensing signal and is used as an event trigger source, a B layer of metabolic countering flora is used for decoupling conversion of disease-promoting saponin and controllable release of a metabolic false substrate, and a C layer of functional florae is used for controlling root rot of panax notoginseng. The C-layer self-healing conductive biological membrane construction flora forms a biological membrane with conductivity and self-healing capability on the root surface, and the D-layer electrically-enabled flora constructs stable biological micro-current at the rhizosphere by using ferric oxide particles as external electron acceptors. The layer A triggers to cause controlled release of the layer B, a conversion product of the layer B provides a carbon source and a structural unit for the layer C, and a conductive network of the layer C and the layer D form an electronic path. The biological agent is used for early recognition and continuous inhibition of pathogens in panax notoginseng planting soil, can start defense response in the early stage of quorum sensing signal occurrence, and keeps action stability under complex environmental conditions.

The present application relates to the technical field of resource utilization of fruit tree branches, and provides a method for returning citrus branches to the orchard, which comprises the following steps: (1) crushing and fermenting the pruned citrus branches, and mixing the branches with organic fertilizer to obtain material A; (2) digging a ditch along a direction parallel to the tangent of the tree crown drip line; and (3) applying the material A obtained in the step (2) into the ditch, and then covering the ditch with soil. The method for returning citrus branches to the orchard can significantly improve the diversity of arbuscular mycorrhizal fungi and the stability of the community structure in the soil, and promote more arbuscular mycorrhizal fungi to act on the rhizosphere soil and root hairs of citrus, so as to effectively improve the physicochemical properties of the soil and promote the growth and development of citrus. The method for returning citrus branches to the orchard is a resource utilization of waste, and can better promote the green, low-carbon, efficient and sustainable development of the citrus industry.

The invention discloses a seedling raising and transplanting method for mangrove plant Aegiceras corniculatum based on external application of gibberellin, and belongs to the technical field of saline-alkali plant cultivation and mud flat ecological restoration. The method mainly comprises the four stages of seed disinfection and gibberellin soaking, germination cultivation, seedling cultivation, salinity domestication and mud flat transplanting. After being disinfected, the seeds are soaked in a gibberellin solution with the concentration being 300 mg / L, gibberellin with the same concentration is sprayed regularly during germination, and the germination rate and the germination speed are increased. In the seedling stage, humus soil and mud flat bottom mud mixed matrix is adopted for cultivation, and growth is promoted by spraying 100 mg / L gibberellin to leaf surfaces and injecting 25 mg / L gibberellin to rhizosphere. After seedlings are formed, the seedlings are subjected to step-by-step salinity domestication (5 per thousand to 30 per thousand), gibberellin spraying and rhizosphere injection are carried out at regular intervals, and the stress resistance of the seedlings is enhanced. The method can effectively shorten the seedling raising period of Aegiceras corniculatum, improve the seedling raising efficiency, enhance the adaptive capacity of seedlings to the tidal zone environment, and facilitate rapid recovery of a mangrove forest ecosystem.

The invention belongs to the technical field of saline-alkali soil improvement, and particularly relates to a coastal saline-alkali soil-oriented intelligent pH-responsive controlled-release crop growth-promoting soil conditioner as well as a preparation method and application thereof. The modifier is biochar-based composite microspheres, an inner core is formed by compounding biochar, silicon quantum dots and a nitrogen-phosphorus-potassium compound fertilizer, an outer layer is coated with a pH response coating formed by chitosan, and an integrated system of environmental perception, release regulation and accurate fertilizer supply is constructed. After the fertilizer is applied to soil, a coating layer swells under the rhizosphere micro-domain acidic condition, nutrient diffusion is intelligently regulated and controlled, and leaching loss and volatilization are reduced. The biochar porous structure adsorbs and fixes salt ions, improves aggregate and ventilation and water permeability, and enhances water retention and fertilizer conservation; the silicon quantum dots improve the photosynthetic efficiency and enhance the oxidation resistance; the compound fertilizer is continuously supplied to nitrogen, phosphorus and potassium. The components synergistically improve soil microstructure and water and salt migration, relieve salt ion poison, improve nutrient utilization and crop salt tolerance, promote yield increase and stability and are suitable for saline-alkali soil remediation.

This invention discloses a smart irrigation management system based on soil and crop moisture status identification, belonging to the field of crop moisture status monitoring technology. The system includes: acquiring leaf electrophysiological signal sequences and multi-depth water potential parameters of the rhizosphere microdomain of the target crop; determining the onset time of water stress and its corresponding first characteristic frequency based on the leaf electrophysiological signal sequences; determining the active water-uptake layer and its corresponding second characteristic water potential based on the multi-depth water potential parameters; performing a coupled comparison based on the first characteristic frequency and the second characteristic water potential, and determining whether irrigation triggering conditions are met based on the comparison results. If the irrigation triggering conditions are met, a differentiated irrigation quota is determined for different irrigation areas. This invention determines irrigation triggering conditions by comparing the consistency and degree of deviation between the first and second deviation directions, avoiding premature or late irrigation caused by misjudgment based on a single indicator.

The invention discloses a soil column test device and method for plant root zone micro-ecology simulation research. The soil column test device comprises a soil column test model module and a solution collection module. The soil column experimental model module comprises a soil filling container with an upper cover and a lower cover, a plant root simulation device, a simulated root exudate sampling device, a soil oxidation-reduction potential monitoring device and a gas collection device, and the solution collection module is used for collecting liquid in the simulation process. According to the device, the plant root simulating device, the root exudate simulating and sampling device, the soil oxidation-reduction state monitoring device and the gas and soil solution collecting device are integrated, so that comprehensive simulation and monitoring of multiple factors of a plant root zone are realized. The device is low in processing cost, convenient to use and easy to operate, all parts are detachable and reusable, multiple pieces of environmental parameter information can be obtained, and the device has important significance on plant root zone micro-ecology research.

This invention discloses the application of a compound microbial agent S14 in improving the salt tolerance and promoting the growth of potatoes, belonging to the field of microbiology. The compound microbial agent S14 is composed of three bacteria: Lelliottia sp. 132, Leucobacter tardus 153, and Pantoea ananatis 103. This compound microbial agent can effectively promote potato growth and significantly increase the emergence rate, biomass, plant height, and tuber weight of potatoes. The compound microbial agent S14 can not only effectively maintain the ion content of hybrid diploid potato seedlings, but also effectively improve the salt tolerance of potatoes, thereby increasing the tuber weight of the commercial variety Fiurita in saline-alkali soil. The compound microbial agent S14 provided by this invention can improve the salt tolerance of potatoes and is suitable for potato cultivation in saline-alkali soil, belonging to the field of plant rhizosphere growth-promoting bacteria technology.

This invention belongs to the field of microbial technology, specifically relating to a compound microbial fertilizer that synergistically regulates rhizosphere flora and nitrogen metabolism genes and its application. The microbial agent includes: Bacillus belye (… Bacillus velezensis ), Bacillus subtilis subsp. subtilis ( Bacillus subtilis subsp. subtilis ) and Linyi Streptomyces ( Streptomyces linyiensis Two or three of the following. The microbial agent provided by this invention has significant growth-promoting and nitrogen-fixing effects when applied to crops. It can maintain or even improve soil fertility and crop yield under the condition of reducing nitrogen fertilizer application by 30%, providing a green and efficient new solution for the implementation of "nitrogen reduction and efficiency enhancement".

The invention discloses a thick-stem high-yield caulis spatholobi germplasm screening and breeding method suitable for an under-forest habitat. According to the method, a standardized under-forest cultivation system is constructed, spiral winding induction synergistically mediated by plant morphology and plant hormones is implemented, lignification development of stems is enhanced by applying mild mechanical stress with specific parameters, rhizosphere microorganism regulation and systematic nutrition management are synchronously combined, and the yield of the stems is increased. And finally, a multi-dimensional comprehensive evaluation system covering the stem shape, the wood density, the active ingredient content and the under-forest weak light adaptability is established, so that an integrated technical scheme for efficiently and accurately breeding the excellent caulis spatholobi germplasm with thick stem phenotype, high flavone accumulation and strong under-forest weak light tolerance from a large number of candidate germplasm is realized.

The invention discloses a moringa oleifera peptide-based cadmium blocking agent and application thereof. The preparation is composed of moringa oleifera peptide (the mass volume concentration is 30-120 mg / L), a compound stabilizer (the mass volume concentration is 20-60 mg / L) and a phosphate buffer solution, the compound stabilizer is composed of sodium alginate and nano silicon dioxide according to the mass ratio of 1: (1.0-1.5), and the pH value of the preparation is 6.0-7.0. The moringa oleifera peptide is innovatively applied to cadmium prevention and control of Chinese cabbages in northern alkaline cadmium-polluted soil (pH is 6.5-7.5), accurate regulation and control are achieved in a rhizosphere irrigation mode, a sodium alginate-nano silicon dioxide composite stabilizer system synergistically plays the dual functions of dispersion stability and slow release protection, and it is ensured that the moringa oleifera peptide keeps activity in the soil environment. The preparation has the advantages of being small in dosage, low in cost, easy and convenient to operate, environmentally friendly and the like, a scientific and feasible technical scheme is provided for safe utilization of cadmium-polluted farmlands in northern alkaline soil areas, and the preparation has important application value and popularization prospects.

The application discloses a kind of efficient cultivation and intelligent control method for relieving continuous cropping obstacles of Lanzhou white lan guava, belong to agricultural planting technical field, the method includes: before planting, soil base improvement is carried out by being input mushroom residue organic conditioning agent and oil residue compound organic fertilizer, before planting, fertilization ditch is excavated, after being input biological base material coated slow-release fertilizer, it is covered with soil in ditch bottom, compound microbial inoculum carrier granule is input in middle upper part, and the root layer nutrient gradient structure of " bottom slow-release nutrient library-middle layer microbial activity layer-surface rhizosphere nutrient area" is constructed, when planting, it is supplemented with inoculum dip root treatment, and soil sensor and in-situ microbial activity monitoring chip are arranged in field, and soil moisture regime, nutrient and microbial activity data are collected in real time, when microbial activity is lower than threshold value, automatically supplement nutrient solution, and intelligent decision system generates fertilization instruction;Fruit swelling stage is divided into stages and precision topdressing is carried out;After harvesting, melon seedling is returned to field and green manure is planted.

The invention provides a planting method for promoting synthesis of camptothecin and derivatives thereof from nothapodytes pittosporoides. The planting method comprises the following steps: planting a plurality of nothapodytes pittosporoides trees in a planting field; organic fertilizer is applied to the multiple planted nothapodytes pittosporoides trees every first preset time; the planted nothapodytes pittosporoides trees are soaked with cold water for several days, and temperature and water stress is carried out; removing the nothapodytes nothapodytes which do not survive after water stress; introducing microorganisms every second preset time to adjust rhizosphere microorganisms of the nothapodytes pittosporoides trees so as to carry out microorganism stress; watering is stopped in autumn, so that the survival nothapodytes nothapodytes trees are subjected to sunlight stress, and the survival nothapodytes nothapodytes trees enter the sunlight stress period; in the sunlight stress period, synthesis of terpene indole alkaloid of rhizosphere microorganisms is activated, and synthesis of camptothecin and derivatives of camptothecin from nothapodytes pittosporoides is promoted; wherein except for the sunlight stress period, the environment temperature of the planting field is kept at 5 DEG C or above, and the environment humidity is kept at 63%-78%.