27 results about "Rhizobacteria" patented technology

The invention discloses actinomucor elegans EM-27, a microbial agent and application, and belongs to the technical field of microbial application. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41924. According to the invention, an actinomucor elegans strain is separated from wheat rhizosphere, and antagonism experiments find that 8.7% of rhizosphere bacteria have antagonism on the actinomucor elegans, and 91.3% of rhizosphere bacteria and actinomucor elegans can coexist. And by applying actinomucor elegans, the growth and development of wheat can be obviously promoted. Therefore, the actinomucor elegans has a good application prospect in the aspect of improving plant growth and development.

The invention discloses a plant growth-promoting bacterium and application thereof, relates to the field of microorganisms, and provides a plant growth-promoting bacterium which can generate a growth-promoting effect on corn plants and further improve the growth condition of the corn plants. The growth-promoting bacterium is pumilus minutus E2 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University in Wuhan, the preservation date is September 19, 2025, and the preservation number is CCTCC NO: M 20252071. By applying the strain to a corn culture medium, the bifurcation number, the root tip number, the root length, the dry weight, the fresh weight and the plant height of corn can be increased, the growth promoting effect of the strain on corn plants is proved, and a basic support is provided for further application to production.

The invention discloses a growth-promoting rhizosphere bacterium HB-63 and application thereof, and belongs to the technical field of agricultural microorganisms. The growth-promoting rhizobacteria HB-63 is bacillus aryabhattai and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the growth-promoting rhizobacteria HB-63 is GDMCC (Guangdong Microbial Culture Collection Center) NO: 67117. The invention discloses a culture containing the growth-promoting rhizobacteria HB-63 and a microbial agent containing the growth-promoting rhizobacteria HB-63 or the culture of the growth-promoting rhizobacteria HB-63. The invention also discloses application of the growth-promoting rhizobacteria HB-63 or the culture thereof or the microbial agent containing the growth-promoting rhizobacteria HB-63, and the application comprises application in products for promoting the development of epimedium root systems or increasing the content of medicinal components. According to the invention, the growth-promoting rhizobacteria HB-63 with a growth-promoting effect on epimedium is found for the first time. Compared with an existing plant growth-promoting bacterium with a single function, the strain HB-63 has the outstanding advantages of efficiently promoting plant growth and remarkably improving the quality of medicinal materials, and has an important promoting effect on guaranteeing the quality of traditional Chinese medicinal materials and improving industrial economic benefits.

The invention relates to the technical field of plant cultivation, in particular to a high-resistance plant cultivation method suitable for an acidic potassium feldspar mine. Comprising the following steps: screening a symbiotic synergistic plant group: selecting a potassium-enriched plant, an aluminum toxicity-resistant plant and a growth-promoting rhizobacteria carrier plant; constructing a layered matrix: sequentially laying an isolating layer, an improved layer and a growth layer on the mine surface; inoculating acid-resistant growth-promoting rhizobacteria: inoculating the acid-resistant strain to the root system of the carrier plant; planting by division of labor according to functions: arranging potassium-enriched plants and aluminum toxicity-resistant plants at intervals, and planting carrier plants at the periphery; through cooperative planting of symbiotic cooperative plant groups, in cooperation with three-dimensional resistance control and acid-base regulation and control of layered substrates and targeted inoculation of acid-resistant rhizosphere growth-promoting bacteria to carrier plant root systems, biological adsorption passivation of aluminum ions, biological activation of mineral potassium and cooperative optimization of rhizosphere microdomain pH are achieved, and the yield of the plant growth-promoting agent is increased. Therefore, the problems of aluminum toxicity stress and low potassium bioavailability of the acidic potassium feldspar mine are systematically solved.

The application discloses a kind of plant salt stress tolerance improving rhizosphere growth promoting bacteria composition and application thereof, belong to microbial application technical field.The rhizosphere growth promoting bacteria composition of the application includes Bacillus subtilis BK73 with accession number CGMCC NO.32725 and Enterobacter ludwigii FZ20 with accession number CGMCC NO.32550.Bacillus subtilis BK73 has the activity of producing ACC deaminase, can synthesize gamma-polyglutamic acid, and Enterobacter ludwigii FZ20 has the ability of nitrogen fixation, phosphorus dissolution and IAA production.Plant experiments show that the rhizosphere growth promoting bacteria composition provided by the application exerts the synergistic effect between strains, and has obvious promoting effect on the germination of corn seeds under salt stress, the growth of corn plants and the salt stress tolerance of corn plants.The application provides a new growth promoting bacteria composition for improving plant salt tolerance, and has wide application prospect.

The invention relates to a rice growth-promoting rhizobacteria for activating insoluble phosphorus in red soil and application of the rice growth-promoting rhizobacteria. The invention discloses a Pandoraea sp. PanRPS strain with phosphorus dissolving capacity in acid red soil and application of the Pandoraea sp. PanRPS strain. The strain PanRPS can take insoluble iron phosphate as a substrate to activate phosphorus in the insoluble iron phosphate, so that the growth of rice under low phosphorus stress is promoted. The functional strain provided by the invention is of great significance in relieving low-phosphorus stress in a rice planting system in an acid red soil medium-low yield field, promoting rice growth and increasing the rice yield in a red soil area.

Biofertilizer granule comprising a core and a coating, said core comprises at least one fertilizer chosen in the group comprising inorganic fertilizer, organic fertilizers, biofertilizer, and their mixture, said coating comprises at least a binding agent and a soil activator composition having an extended shelf-life duration, said soil activator composition comprising at least a carrier and at least a bacterial mixture containing at least a rhizobacteria species being a Bacillus subtilis and at least a bacteria species with some antifungal activity being different from Bacillus subtilis and being a Pseudomonas monteilii species, said bacterial mixture containing at least 263 million (2.63x108) cfu total bacteria / g soil activator composition after said extended shelf life measured according to ASTM-D5465- 93 (dated 1998) standard.

The invention discloses a method for synergistically improving nitrogen utilization efficiency and fixing carbon and reducing emission based on biochar and a microbial agent. The method comprises the following steps: providing biochar; arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria are provided; the biochar is applied before crops are sown, and the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are applied before the crops are sown or when the crops are sown; field management; and evaluating carbon sequestration and emission reduction. The biochar provides a habitat for arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are protected, nutrients are adsorbed through the porous structure and surface characteristics of the biochar, and loss is reduced; the arbuscular mycorrhizal fungus hypha network enlarges the root system absorption range, especially promotes absorption of phosphorus and other difficult-to-move nutrients, and indirectly promotes nitrogen metabolism; the plant growth-promoting rhizobacteria can fix nitrogen, dissolve phosphorus, secrete growth hormone and directly promote plant growth and nitrogen absorption. The three components cooperate with each other to create an optimal rhizosphere microenvironment, so that nitrogen supply and crop requirements are better matched in time and space.

The invention discloses a method for cinnamomum camphora adventitious root suspension culture and efficient d-borneol accumulation, and belongs to the technical field of plant tissue culture. The invention discloses a method for cinnamomum camphora adventitious root suspension culture and efficient d-borneol accumulation, which comprises the following steps: establishing a cinnamomum camphora sterile regeneration system to obtain sterile seedlings, inducing the sterile seedlings to generate adventitious roots, preparing a plant growth-promoting rhizobacteria PGPR extracting solution, and culturing and domesticating the adventitious roots by using a suspension culture medium containing the PGPR extracting solution. The domesticated adventitious roots are subjected to efficient d-borneol accumulation culture in an induction culture medium, and the accumulated d-borneol is extracted and detected. The method effectively solves the problems of long production period, low yield and unstable components of the traditional method, and provides reliable technical support for realizing large-scale and green production of the d-borneol.

The invention discloses a plant growth-promoting bacterium specific primer pair, a kit and application. The plant growth-promoting bacterium specific primer pair is composed of an upstream primer P463-F as shown in SEQ ID NO. 1 and a downstream primer P463-R2 as shown in SEQ ID NO. 2. According to the present invention, the plant growth-promoting bacterium specific primer pair is used for identifying the plant growth-promoting bacterium Penicillium sp.PQxj3, such that the survival state of the Penicillium sp.PQxj3 in the soil can be monitored in real time, the application conditions of the Penicillium sp.PQxj3 in different environments, different crops and different soils can be optimized, and the optimal effect of plant growth promotion can be achieved.

The present invention relates to the technical field of the environmental microbiology, especially to culture medium for high-throughput separation of rhizobacteria from crops, preparation method and application thereof. The culture medium for high-throughput separation of crop rhizobacteria comprises a 1 / l0 TSB medium and a culture medium additive (solution), wherein culture medium additive (solution) is obtained by mixing the crop plants with TSB medium and then by crushing, fermenting, filtering as well as sterilizing. By utilizing the interaction network between multiple microorganisms, crop-source nutrients are fermented using microbiome in-situ in the rhizosphere, and the fermented products are used as carbon and nitrogen source for separation of rhizosphere microorganisms, which can simulate the in-situ environment of rhizosphere microorganisms to the maximum extent, thereby obtaining more microbial species. Combined with the high-throughput separation method, culture medium provided by present invention can obtain more than 60% of bacterial species obtained by high-throughput sequencing.

The present invention provides for a plant-growth-promoting rhizobacterium (PGPR) genetically modified such that the PGPR is capable of colonizing the root of a plurality of plant species, such as a plurality of crop plant species, wherein the genetically modified PGPR is enhanced in the capability to colonize the root of a plurality of plant species when compared to a wild-type or unmodified PGPR.

The present application relates to the field of microbial technology, and specifically provides a plant growth promoting rhizobacteria NFB10 and its application. The strain is isolated from the rhizosphere soil of rubber tree in Hainan province, and is a potential new species of Pseudomonas. The similarity of 16S rDNA sequence of the strain to similar strains is higher than 99.8%, but the average nucleotide identity of whole genome between species is lower than the inter-species threshold of 95%. The strain has the abilities of phosphorus solubilization, potassium solubilization and nitrogen fixation, and can produce indole acetic acid, cellulase, amylase and ammonia and other growth promoting substances. The strain grows well in the salt concentration of 3% or less and the pH range of 5.0-9.0. Experiments show that the strain can significantly improve the germination rate, root length and sprout length of wheat seeds, and has obvious promoting effects on the plant height, ground diameter, biomass and chlorophyll content of the leaves of the seedlings of Hevea brasiliensis. The strain can be used for preparing plant growth promoting bacteria agents, and is suitable for green cultivation and yield improvement of rubber trees and other crops.

The present invention relates to a new Bacillus velezensis strain with deposit number CECT 30599 as well as uses thereof in agriculture. Specifically, the present invention relates to the use of the B. velezensis strain CECT 30599 to improve the resistance of a plant to drought, promote germination in a plant, promote the growth of a plant, the control of pathogenic microorganisms in a plant, reduce the water supply to a plant, promote flowering in a plant, and / or increase the population of plant growth-promoting rhizobacteria in the soil where a plant is grown.

The invention discloses an atractylodes macrocephala root rot biocontrol bacterium and application thereof, and belongs to the technical field of plant disease biocontrol. The rhizoma atractylodis macrocephalae rhizosphere bacterium is separated from healthy rhizosphere soil of a compositae plant rhizoma atractylodis macrocephalae by adopting a rhizosphere bacterium separation and purification technology, is classified and named as Pantoea rodsii RSB15, is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M20251954, and the preservation date is September 1, 2025. The invention also discloses application of the bacterium: the bacterium can effectively inhibit the growth of pathogenic bacteria of bighead atractylodes rhizome root rot, and the inhibition rate of plate confrontation culture reaches 20.57%; the morphological structure of pathogenic fungi can be destroyed. The bacterium provided by the invention has an obvious effect of preventing and treating bighead atractylodes rhizome root rot and provides a wide application prospect in the field of biological prevention and treatment of bighead atractylodes rhizome fungal diseases.

The application discloses application of an iolE gene in improving the symbiotic nitrogen fixation capacity of plant growth promoting bacteria, and application of the iolE gene or a protein coded by the iolE gene in at least one of the following (a1)-(a3): (a1) improving the symbiotic nitrogen fixation capacity of plant growth promoting bacteria; (a2) improving the efficiency of nodule formation and nitrogen fixation of host plants by plant growth promoting bacteria assisting rhizobium; and (a3) constructing recombinant bacteria with improved symbiotic nitrogen fixation capacity. The discovery of the application can be used for targeted modification of a target gene iolE, and provides a theoretical basis for development of high-efficiency genetically engineered bacterial agents; can be applied to research on other PGPR functional genes, and promotes the development of agricultural microbial technology.

The invention relates to the technical field of microorganisms, and particularly provides application of trichoderma asperellum MM7 in constructing a drought-resistant banana rhizosphere microflora and improving the drought resistance of bananas. The trichoderma asperellum MM7 inoculation can relieve the inhibition of drought stress on the growth of banana seedlings and improve the water content and survival rate of tissues; the root antioxidant enzyme activity is enhanced, the accumulation of osmotic regulation substances is promoted, and the membrane lipid peroxidation degree is reduced; the expression of ABA metabolism and signal channel related genes and aquaporin genes is up-regulated; a rhizosphere bacterial community structure under drought stress is remodeled, beneficial functional bacteria such as pseudomonas, streptomyces and nitrococcus are remarkably enriched, and the drought resisting function of the bacillus amyloliquefaciens depends on indigenous microbial communities. The invention reveals that the trichoderma asperellum MM7 provides a new strain resource for developing a banana drought-resistant biological agent based on microbiome regulation through a synergistic interaction mechanism of directly regulating host physiology and gene expression-indirectly remodeling rhizosphere beneficial microflora.

The invention relates to the technical field of fertilizers, in particular to a nitrogen-fixing microbial fertilizer and a preparation method thereof. The nitrogen-fixing microbial fertilizer is prepared from the following components in percentage: 2 to 5 percent of plant growth-promoting rhizobacteria fungicide, 2 to 5 percent of humic acid, 15 to 20 percent of straw powder, 15 to 20 percent of cellulose, 20 to 30 percent of wheat starch, 1 to 3 percent of nitrifying bacteria, 2 to 5 percent of indoleacetic acid and 10 to 15 percent of diatomite. The prepared nitrogen-fixing microbial fertilizer can effectively improve soil nitrogen circulation and promote organic nitrogen mineralization through the nitrogen-fixing microbial effect, the content of ammonium nitrogen and nitrate nitrogen in soil is increased, the nitrogen-fixing microbial fertilizer is especially suitable for improvement of barren soil, meanwhile, a stable nitrogen source can be directly provided, healthy growth of root systems, stems and leaves of plants can be promoted, and the yield of the plants is increased. Particularly, the growth speed and yield of crops can be remarkably improved in soil lacking nitrogen element, the use of chemical nitrogen fertilizer can be reduced, the fertilizer cost in agricultural production can be reduced, and the negative influence of the nitrogen fertilizer on the environment can be reduced.

The nano-symbiotic catalyst described herein includes a mixture of a carbon source, water, microbes, a nano-sized organo-mineral complex known as Montmorillonite Alginate, and humic / fulvic acids. Microbes used in the nano-symbiotic catalyst may include microbes classified as Plant growth Promoting Rhizobacteria (PGPR) and Plant Growth Promoting Fungi (PGPF). The nano-symbiotic catalyst enhances the speed at which a beneficial microbe and plant form a symbiotically mutual relationship. Due to the nano-size and configuration of the nutrients, plant reception of these nutrients is increased while the amount of energy used to form this relationship and acquire beneficial nutrients is decreased. The nano-symbiotic catalyst may be used with multiple crops, soil types, and environmental conditions to reduce input costs and resources, increase agriculture production and quality, and enhance regenerative agriculture practices.

This invention discloses a method for enhancing cucumber resistance to Fusarium wilt by driving the accumulation of rhizosphere bacteria using Trichoderma echinococcus FJ035, belonging to the field of plant disease biological control technology. The method involves applying Trichoderma echinococcus FJ035, Nitro-reducing Pseudomonas 22, and Bacillus vesicularis 33 to the cucumber rhizosphere via root irrigation. This method offers stable efficacy, is environmentally friendly, and can replace or reduce the use of chemical pesticides. The control efficacy in potted plants reaches 77.97%, and in the field, it reaches 68.42%, comparable to the efficacy of commonly used chemical pesticides such as hymexazol and thiophanate-methyl, demonstrating promising prospects for industrial application.

The invention discloses a plant growth-promoting rhizobacteria and a microorganism in-situ strengthening method for vegetable production, and belongs to the technical field of agriculture and microorganisms. The classification name of the plant growth-promoting rhizobacteria is Lelliottia sp, the preservation unit is China General Microbiological Culture Collection Center, which is called CGMCC for short, the preservation name is KY49, the preservation date is August 15, 2025, and the preservation number is CGMCC No.35639. The invention further discloses a preparation method of the plant growth-promoting rhizobacteria. The microbial agent is applied to the pakchoi planted in the selenium-rich soil in a root irrigation mode, the microbial agent is applied 2 days after the pakchoi is transplanted, the application amount of the microbial agent is 8-10 mL / plant, and the concentration of the microbial agent is (1.8-2) * 10 < 8 > CFU / mL. According to the method, the microbial inoculum is applied to selenium-rich soil, the yield of pakchoi is increased by 50%, the selenium content is increased by 65%, reaches 0.12 mg / kg (dw) and exceeds the standard of selenium-rich vegetables, and the selenium supplement requirement of people is met.

The invention relates to the technical field of microorganisms, and particularly provides a plant growth-promoting rhizobacteria SPN5 and application thereof. The strain is separated from rhizosphere soil of rubber trees in Hainan Province and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 66466. Through identification, the SPN5 is a potential new species in pantoea, has various plant growth promoting capacities of phosphate solubilization, potassium solubilization, nitrogen fixation, siderophore production, cellulase production, indoleacetic acid production, ammonia production and the like, and can well grow in the salt concentration of 3% or below and the pH range of 5.0-9.0. Pot experiments show that the SPN5 has a remarkable growth promoting effect on lettuce and rubber tree seedlings, plant biomass, leaf chlorophyll content and crop quality can be improved, and growth of rubber trees can still be effectively promoted especially under the condition that application of chemical fertilizer is reduced by 20%-40%. The strain is suitable for preparing a microbial agent, and has a good application prospect in the aspects of promoting crop growth and realizing reduction and synergism of chemical fertilizers.

The invention relates to the technical field of microorganisms, and particularly provides a plant growth-promoting rhizobacteria SYR43 and application thereof. The strain is a new bacillus strain which is separated from a high-yield rubber tree rhizosphere soil environment, and is preserved in Guangdong Microbial Culture Collection Center on June 4, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 66467. The strain has the capacity of producing ammonia and protease, and can well grow in the salt concentration of 1% or below and the pH of 5.0-8.0; through morphological, physiological and biochemical and molecular biological identification, the strain is a potential new strain of the novel bacillus. Pot experiment verification shows that the strain can significantly improve the chlorophyll content and photosynthetic ability of arabidopsis thaliana, lettuce and rubber tree seedlings, and promote plant biomass growth and root development. The invention provides a special growth-promoting functional strain resource for plants such as hevea brasiliensis and the like, and has important significance for improving the benefits of natural rubber industry in China.

The invention relates to the technical field of microorganisms, and particularly discloses a plant growth-promoting rhizosphere strain ST85 and application thereof. The plant growth-promoting rhizosphere bacterial strain ST85 is preserved in the China General Microbiological Culture Collection Center on March 14, 2025, the classification name of the plant growth-promoting rhizosphere bacterial strain ST85 is variovorax boronics, and the preservation number of the plant growth-promoting rhizosphere bacterial strain ST85 is CGMCC No. 33814. The ST85 strain can enter the interior of the root system of the potato and is successfully colonized, so that the ST85 strain has relatively strong colonization capability. The strain has growth promoting characteristics of secreting siderophores, solubilizing phosphorus, fixing nitrogen, cellulase, xylanase and the like; and the strain can degrade autotoxic substances such as vanillin, ferulic acid and p-hydroxybenzoic acid in continuous cropping potato soil, and is widely applied as an agricultural soil inoculant.

A system for evaluating the plant growth-promoting rhizobacterial properties (PGPR) of endophytic bacteria isolated from Acanthus ilicifolius L., and for assessing plant growth parameters, consisting of: a) an isolation unit for isolating and purifying endophytic bacteria from leaves of Acanthus ilicifolius L., the isolation unit comprising: • Surface sterilization device configured to treat leaf explants successively with Tween20 solution, 70% ethanol and 1% mercuric chloride, • Grinding device for homogenizing leaf tissue with phosphate buffer solution, • Incubation chamber configured for the cultivation of bacterial colonies on nutrient agar at 37°C; b) a characterization unit for the morphological, biochemical and molecular characterization of isolated bacteria, wherein the characterization unit comprises the following: • Equipment for Gram staining, • Equipment for biochemical tests for performing the indole test, the methyl red test, the citrate utilization test, the H2S production test, the gelatin hydrolysis test, the starch hydrolysis test and the catalase test, and • Molecular analysis equipment configured for 16S rRNA sequencing, including DNA isolation apparatus, PCR thermocycler and DNA sequencer; c) an optimization unit configured to analyze the culture conditions for maximum bacterial growth at different temperatures and pH values, the optimization unit comprising a colorimeter configured to measure absorbance at 610 nm to generate bacterial growth curves; d) a PGPR property evaluation unit for assessing the plant growth-promoting potential of isolated bacteria, wherein the PGPR property evaluation unit comprises the following: • Testing equipment for indole-3-acetic acid (IAA) production using the Salkowski color test, • Test apparatus for phosphate solubilization using the Pikovskaya medium • Devices for testing siderophore production using chromazurol S-agar (CAS agar) and • Test apparatus for the activity of 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase using Dworkin and Foster minimal salt medium; and e) a plant growth evaluation unit configured for in vitro analysis of the effects of bacterial inoculum on plant growth parameters such as stem length, root length, leaf length and chlorophyll content.

The present invention relates to a consortium of isolated bacteria that has an application in agriculture. In particular, the described rhizospheric and / or endophytic bacteria can provide a benefit to an agricultural plant derived from a seed, a seedling, a cutting, a seedling, or any plant tissue.

A soil treatment composition capable of recruiting native diazotrophs or other nitrogen fixing organisms to a desired soil site. In one example, a soil treatment composition includes a combination of plant growth promoting rhizobacteria (PGPR) and a nutrient enhancer for attracting native diazotrophs to a desired soil site. The treatment compositions may be mixed or otherwise blended in a tank prior to, or during, a spraying operation. Once mixed, the treatment compositions may be applied to a desired soil site via numerous applications, e.g., in-furrow, 2x2, sidedress, broadcast, or through fertigation. After being applied, such compositions may effectively recruit native diazotrophs which, in turn, may increase nutrient availability, uptake, and utilization.