53 results about "Rhizobacteria" patented technology

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The invention discloses a method for immobilizing growth-promoting rhizobacteria by taking decarburized gangue-based biochar as a carrier, a solid waste-based microorganism immobilization ball prepared by the method and application of the solid waste-based microorganism immobilization ball, and relates to the technical field of solid waste utilization. The method comprises the following steps: preparing gangue-based zeolite by microwave activation and decarburization of coal gangue; preparing gangue-based biochar by utilizing phosphorus modification; and preparing the solid-waste-based microorganism solidification balls by utilizing an adsorption-embedding-crosslinking method. The decarburized gangue-based biochar is used as a carrier material for immobilizing the growth-promoting rhizosphere bacteria, and is used for restoring the sandy degraded soil in the northwest arid mining area. According to the technology, the survival time and functional activity of the growth-promoting rhizobacteria in the soil environment can be improved, and a new way is provided for consumption of bulk gangue solid waste. The method also has the technical advantages of improving the carbon source utilization rate of the gangue and increasing the phosphate fertilizer and the organic carbon library of the soil.

The invention discloses actinomucor elegans EM-27, a microbial agent and application, and belongs to the technical field of microbial application. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.41924. According to the invention, an actinomucor elegans strain is separated from wheat rhizosphere, and antagonism experiments find that 8.7% of rhizosphere bacteria have antagonism on the actinomucor elegans, and 91.3% of rhizosphere bacteria and actinomucor elegans can coexist. And by applying actinomucor elegans, the growth and development of wheat can be obviously promoted. Therefore, the actinomucor elegans has a good application prospect in the aspect of improving plant growth and development.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention relates to the technical field of agricultural micro-ecological application, and particularly discloses a composite combined inoculation method using arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, which comprises the following steps: selecting corn plant seeds without plant diseases and insect pests; a corn planting land is randomly divided into land parcels with the area of 6 m < 2 > (2 m * 3 m), a buffer zone of 1.0 m is arranged between every two adjacent land parcels, and the cultivation mode is flat cropping; the method comprises the following steps: planting arbuscular mycorrhizal fungi inoculum comprising mycorrhiza, dry sand containing spores and ectotrophic hyphae and corn seeds in soil by using a seeder during corn sowing, and after the corn is planted for 30 days, inoculating the plant growth-promoting rhizobacteria inoculum into plants and soil by using a flood irrigation root irrigation method, when the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are inoculated together, the corn rhizosphere microbial community can be changed, the richness and diversity of fungi and bacterial communities in soil are increased, and the relative abundance of plant pathogens in the soil is reduced.

The content of IAA produced by the enterobacter ludwigii XL0404 can reach 18.94 mg / L, the content of ACC deaminase produced by the enterobacter ludwigii XL0404 is 484.48 U / L, and the enterobacter ludwigii XL0404 has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing siderophore and the like, has an inhibition effect on main root and stem disease pathogenic bacteria such as phytophthora nicotianae, rhizoctonia solani, rhizoctonia tectorum, fusarium oxysporum, fusarium solani and the like, and has an inhibition rate of 41.20-55.88%; the germination rate of the tobacco seeds is increased through treatment of the strain XL0404, and the root length is remarkably promoted; the root activity, the whole plant fresh weight, the overground fresh weight, the root fresh weight, the maximum leaf length and the maximum leaf width of the tobacco seedlings treated by the strain XL0404 with the bacterium content of 1 * 10 < 7 > cfu / mL in a seedling culture substrate are obviously higher than those of the tobacco seedlings treated by contrast treatment, and the strain XL0404 can be used as plant growth-promoting rhizobacteria and has a good application prospect in research and development of microbial fertilizers and biological prevention and control of tobacco rhizome diseases.

The invention relates to a plant growth-promoting rhizobacteria. The preservation number of the plant growth-promoting rhizobacteria is GDMCC No: 65494. Through rhizosphere soil microorganism sampling of Gannan navel oranges, the strain G41 with the characteristics of producing indoleacetic acid, producing siderophores and solubilizing phosphorus is successfully separated out. Furthermore, pot experiments verify that the strain group is inoculated, so that the plant height, stem diameter, biomass, chlorophyll content and antioxidant enzyme activity of navel orange seedlings are increased, and the strain has an excellent growth promoting effect. Through strain identification, the strain is formally named as the bacillus subtilis G41, and the bacillus subtilis G41 is named as the bacillus subtilis G41. In particular, it is worth mentioning that the strain has rich gene resources, 32 genes related to growth promoting characteristics are excavated from the gene level, and the genes are widely distributed in key processes such as indoleacetic acid biosynthesis, siderophore biosynthesis and transport and phosphorus circulation.

The invention relates to the technical field of ecological restoration, in particular to a method for improving the transplanting survival rate of saplings in a hydro-fluctuation belt of a hydropower station reservoir area in a dry-hot valley area, which comprises the following steps: mixing a composite slow-release fungicide composition, saplings and seedling raising soil, putting the mixture into planting pits, and maintaining; the composition comprises the following raw materials: arbuscular mycorrhizal fungus powder, plant growth-promoting rhizobacteria, an alginate aqueous solution, a salicylate aqueous solution and a calcium chloride solution. The preparation method of the composition comprises the following steps: mixing the arbuscular mycorrhizal fungi, the plant growth-promoting rhizobacteria and the salicylate aqueous solution to obtain a mixture; dropwise adding the mixture into an alginate aqueous solution to form a mixed solution; and mixing the mixed solution with a calcium chloride aqueous solution, and curing to obtain the composite slow-release fungicide composition. The composite slow-release fungicide composition is mixed with saplings, so that the survival rate of the saplings is increased, and the ecological restoration rate of a hydropower station reservoir area in a dry and hot valley area is increased.

The invention discloses a plant growth-promoting rhizobacteria and application thereof, and relates to the technical field of agricultural microorganisms. The strain is named as serratia sp. SYPP26, the strain is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO: 64911, and the preservation date is July 24, 2024, and the strain is named as serratia sp. SYPP26. The strain is named as serratia sp. SYPP26 and is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO: 64911. The strain is separated from the rhizosphere soil environment of the high-yield rubber tree, is high in environmental adaptability, keeps good growth consistency when the salt concentration is 1-7%, the temperature is 20-30 DEG C and the pH is 3.5-9.0, and can adapt to most of acid soil in the southern China; the strain has the capabilities of dissolving phosphorus, producing siderophores, producing indoleacetic acid, producing gibberellin, producing ammonia and the like, and experiments prove that the strain has the capability of improving the content of ammonium nitrogen and nitrate nitrogen in soil, and can improve the germination rate of rice seeds and remarkably promote plant growth and root development.

The invention relates to the field of growth promoting rhizobacteria (PGPR), in particular to Burkholderia territorii D4-36 and application of the Burkholderia territorii D4-36. The Burkholderia territorii D4-36 is prepared from Burkholderia territorii D4-36 The strain is Burkholderia territorii D4-36, and is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms; the Burkholderia territorii D4-36 is separated into the corn rhizosphere soil, the growth temperature range is large, the Burkholderia territorii D4-36 can easily adapt to various soil environments, VOCs can be generated to promote the growth of cherry seedlings, the expression quantity of IAA synthesis related genes at the roots of the cherry seedlings can be increased, and the rhizosphere soil microbial community structure of the Burkholderia territorii D4-36 can be changed to promote the growth of the cherry seedlings. Therefore, the strain can be used in crop production, not only can promote crop growth, but also has important significance for green sustainable agricultural development.

The invention discloses a growth-promoting rhizobacteria composition for improving salt stress resistance of plants and application of the growth-promoting rhizobacteria composition, and belongs to the technical field of application of microorganisms. The growth-promoting rhizobacteria composition disclosed by the invention comprises bacillus subtilis BK73 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.32725 and enterobacter lutei FZ20 of which the preservation number is CGMCC NO.32550. The invention further discloses a preparation method of the growth-promoting rhizobacteria composition. The bacillus subtilis BK73 has the activity of producing ACC deaminase and can be used for synthesizing gamma-polyglutamic acid, and the enterobacter lewis FZ20 has the capabilities of fixing nitrogen, dissolving phosphorus and producing IAA (Indoleacetic Acid). Plant experiments show that the plant growth-promoting bacterium composition provided by the invention exerts a synergistic effect among strains, and has an obvious promoting effect on germination of corn seeds, growth of corn plants and salt stress tolerance of the corn plants under salt stress. The invention provides a novel growth-promoting bacterium composition for improving the salt tolerance of plants, and has a wide application prospect.

The invention discloses a plant growth-promoting bacterium and application thereof, relates to the field of microorganisms, and provides a plant growth-promoting bacterium which can generate a growth-promoting effect on corn plants and further improve the growth condition of the corn plants. The growth-promoting bacterium is pumilus minutus E2 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University in Wuhan, the preservation date is September 19, 2025, and the preservation number is CCTCC NO: M 20252071. By applying the strain to a corn culture medium, the bifurcation number, the root tip number, the root length, the dry weight, the fresh weight and the plant height of corn can be increased, the growth promoting effect of the strain on corn plants is proved, and a basic support is provided for further application to production.

The invention discloses a special biological fertilizer for osmanthus fragrans flower and a preparation method and application thereof, and belongs to the technical field of biological fertilizers. The special biological fertilizer is prepared from the following raw materials in percentage by mass: 65% of rape seed cakes, 10% of dry branches and fallen leaves, 0.4% of nano silicon, 5% of seaweed extract, 15% of bone meal, 3.4% of potassium sulfate, 1% of trace elements and 0.2% of growth-promoting rhizobacteria. The biological fertilizer is inoculated to osmanthus fragrans cutting seedlings, and results show that the plant height and the crown breadth of a biological fertilizer group are obviously higher than those of a control group from the second month. The biological fertilizer is inoculated to 2-3-year-old healthy sweet osmanthus seedlings, and results show that the biological fertilizer group is obviously higher than the control group 15 days after first fertilization. The biological fertilizer is inoculated to healthy plants with similar growth conditions of the same sweet-scented osmanthus variety, and results show that the biological fertilizer significantly improves the inflorescence number (+ 50%) of a single sweet-scented osmanthus plant and the flower number (+ 45%) of a single inflorescence; the flower development period is prolonged.

The invention discloses a growth-promoting rhizosphere bacterium HB-63 and application thereof, and belongs to the technical field of agricultural microorganisms. The growth-promoting rhizobacteria HB-63 is bacillus aryabhattai and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the growth-promoting rhizobacteria HB-63 is GDMCC (Guangdong Microbial Culture Collection Center) NO: 67117. The invention discloses a culture containing the growth-promoting rhizobacteria HB-63 and a microbial agent containing the growth-promoting rhizobacteria HB-63 or the culture of the growth-promoting rhizobacteria HB-63. The invention also discloses application of the growth-promoting rhizobacteria HB-63 or the culture thereof or the microbial agent containing the growth-promoting rhizobacteria HB-63, and the application comprises application in products for promoting the development of epimedium root systems or increasing the content of medicinal components. According to the invention, the growth-promoting rhizobacteria HB-63 with a growth-promoting effect on epimedium is found for the first time. Compared with an existing plant growth-promoting bacterium with a single function, the strain HB-63 has the outstanding advantages of efficiently promoting plant growth and remarkably improving the quality of medicinal materials, and has an important promoting effect on guaranteeing the quality of traditional Chinese medicinal materials and improving industrial economic benefits.

Provided are a bacterial strain for promoting plant growth and an application thereof, the bacterial strain being selected from at least one of the following: a strain 1 comprising a 16S rDNA sequence as shown in SEQ ID NO: 1 or a complementary sequence thereof or a sequence at least 85% identical to SEQ ID NO: 1; the strain 2 comprises a 16S rDNA sequence as shown in SEQ ID NO: 2 or comprises a sequence which is at least 85% identical with the sequence as shown in SEQ ID NO: 2; a strain 3 comprising a 16S rDNA sequence as shown in SEQ ID NO: 3 or comprising a sequence at least 85% identical to the sequence as shown in SEQ ID NO: 3; and a strain 4 comprising the 16S rDNA sequence as shown in SEQ ID NO: 4 or comprising a sequence at least 85% identical to SEQ ID NO: 4.

The invention relates to the technical field of plant cultivation, in particular to a high-resistance plant cultivation method suitable for an acidic potassium feldspar mine. Comprising the following steps: screening a symbiotic synergistic plant group: selecting a potassium-enriched plant, an aluminum toxicity-resistant plant and a growth-promoting rhizobacteria carrier plant; constructing a layered matrix: sequentially laying an isolating layer, an improved layer and a growth layer on the mine surface; inoculating acid-resistant growth-promoting rhizobacteria: inoculating the acid-resistant strain to the root system of the carrier plant; planting by division of labor according to functions: arranging potassium-enriched plants and aluminum toxicity-resistant plants at intervals, and planting carrier plants at the periphery; through cooperative planting of symbiotic cooperative plant groups, in cooperation with three-dimensional resistance control and acid-base regulation and control of layered substrates and targeted inoculation of acid-resistant rhizosphere growth-promoting bacteria to carrier plant root systems, biological adsorption passivation of aluminum ions, biological activation of mineral potassium and cooperative optimization of rhizosphere microdomain pH are achieved, and the yield of the plant growth-promoting agent is increased. Therefore, the problems of aluminum toxicity stress and low potassium bioavailability of the acidic potassium feldspar mine are systematically solved.

The invention provides a growth-promoting rhizobacteria for enhancing salt tolerance of wheat and application of the growth-promoting rhizobacteria, and belongs to the technical field of agricultural microorganisms. The invention provides stenotrophomonas maltophilia TJ3-57, and the preservation number of the stenotrophomonas maltophilia TJ3-57 is GDMCC (Graphical Digital Microbial Counter Center) The stenotrophomonas maltophilia TJ3-57 can improve the salt stress resistance of plants, relieve the damage of salt stress to the plants and promote the growth of the plants in the saline-alkali soil, so that the yield of crops in the saline-alkali soil is increased.

A culture medium for high-throughput separation of crop rhizobacteria, and a preparation method therefor and the use thereof. The culture medium comprises a 1 / 10 TSB culture medium and an additive solution of the culture medium, and the additive solution of the culture medium is prepared by means of mixing a crop plant with a TSB culture medium, and pulverizing, fermenting, filtering and sterilizing the mixture. With regard to the culture medium, on the basis of a multispecies interaction theory in rhizosphere microbiome and by means of using the interaction network among multiple microorganisms, crop-derived nutrient components are fermented with in-situ rhizosphere microbiome, and the fermentation product is used as the carbon source and nitrogen source for the separation of rhizosphere microorganisms, thereby simulating the in-situ environment of rhizosphere microorganisms to the maximum, and obtaining more microbial species. High-throughput sequencing reveals that the culture medium can obtain more than 60% of bacterial species compared with an existing culture medium.

The application discloses a kind of plant salt stress tolerance improving rhizosphere growth promoting bacteria composition and application thereof, belong to microbial application technical field.The rhizosphere growth promoting bacteria composition of the application includes Bacillus subtilis BK73 with accession number CGMCC NO.32725 and Enterobacter ludwigii FZ20 with accession number CGMCC NO.32550.Bacillus subtilis BK73 has the activity of producing ACC deaminase, can synthesize gamma-polyglutamic acid, and Enterobacter ludwigii FZ20 has the ability of nitrogen fixation, phosphorus dissolution and IAA production.Plant experiments show that the rhizosphere growth promoting bacteria composition provided by the application exerts the synergistic effect between strains, and has obvious promoting effect on the germination of corn seeds under salt stress, the growth of corn plants and the salt stress tolerance of corn plants.The application provides a new growth promoting bacteria composition for improving plant salt tolerance, and has wide application prospect.

The invention relates to a rice growth-promoting rhizobacteria for activating insoluble phosphorus in red soil and application of the rice growth-promoting rhizobacteria. The invention discloses a Pandoraea sp. PanRPS strain with phosphorus dissolving capacity in acid red soil and application of the Pandoraea sp. PanRPS strain. The strain PanRPS can take insoluble iron phosphate as a substrate to activate phosphorus in the insoluble iron phosphate, so that the growth of rice under low phosphorus stress is promoted. The functional strain provided by the invention is of great significance in relieving low-phosphorus stress in a rice planting system in an acid red soil medium-low yield field, promoting rice growth and increasing the rice yield in a red soil area.

Biofertilizer granule comprising a core and a coating, said core comprises at least one fertilizer chosen in the group comprising inorganic fertilizer, organic fertilizers, biofertilizer, and their mixture, said coating comprises at least a binding agent and a soil activator composition having an extended shelf-life duration, said soil activator composition comprising at least a carrier and at least a bacterial mixture containing at least a rhizobacteria species being a Bacillus subtilis and at least a bacteria species with some antifungal activity being different from Bacillus subtilis and being a Pseudomonas monteilii species, said bacterial mixture containing at least 263 million (2.63x108) cfu total bacteria / g soil activator composition after said extended shelf life measured according to ASTM-D5465- 93 (dated 1998) standard.

Provided are a bacterial strain for promoting plant growth and an application thereof, the bacterial strain being selected from at least one of the following: a strain 1 comprising a 16S rDNA sequence as shown in SEQ ID NO: 1 or a complementary sequence thereof or a sequence at least 85% identical to SEQ ID NO: 1; the strain 2 comprises a 16S rDNA sequence as shown in SEQ ID NO: 2 or comprises a sequence which is at least 85% identical with the sequence as shown in SEQ ID NO: 2; a strain 3 comprising a 16S rDNA sequence as shown in SEQ ID NO: 3 or comprising a sequence at least 85% identical to the sequence as shown in SEQ ID NO: 3; and a strain 4 comprising the 16S rDNA sequence as shown in SEQ ID NO: 4 or comprising a sequence at least 85% identical to SEQ ID NO: 4.

The invention discloses a method for synergistically improving nitrogen utilization efficiency and fixing carbon and reducing emission based on biochar and a microbial agent. The method comprises the following steps: providing biochar; arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria are provided; the biochar is applied before crops are sown, and the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are applied before the crops are sown or when the crops are sown; field management; and evaluating carbon sequestration and emission reduction. The biochar provides a habitat for arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are protected, nutrients are adsorbed through the porous structure and surface characteristics of the biochar, and loss is reduced; the arbuscular mycorrhizal fungus hypha network enlarges the root system absorption range, especially promotes absorption of phosphorus and other difficult-to-move nutrients, and indirectly promotes nitrogen metabolism; the plant growth-promoting rhizobacteria can fix nitrogen, dissolve phosphorus, secrete growth hormone and directly promote plant growth and nitrogen absorption. The three components cooperate with each other to create an optimal rhizosphere microenvironment, so that nitrogen supply and crop requirements are better matched in time and space.

The invention discloses a method for cinnamomum camphora adventitious root suspension culture and efficient d-borneol accumulation, and belongs to the technical field of plant tissue culture. The invention discloses a method for cinnamomum camphora adventitious root suspension culture and efficient d-borneol accumulation, which comprises the following steps: establishing a cinnamomum camphora sterile regeneration system to obtain sterile seedlings, inducing the sterile seedlings to generate adventitious roots, preparing a plant growth-promoting rhizobacteria PGPR extracting solution, and culturing and domesticating the adventitious roots by using a suspension culture medium containing the PGPR extracting solution. The domesticated adventitious roots are subjected to efficient d-borneol accumulation culture in an induction culture medium, and the accumulated d-borneol is extracted and detected. The method effectively solves the problems of long production period, low yield and unstable components of the traditional method, and provides reliable technical support for realizing large-scale and green production of the d-borneol.

The invention discloses a plant growth-promoting bacterium specific primer pair, a kit and application. The plant growth-promoting bacterium specific primer pair is composed of an upstream primer P463-F as shown in SEQ ID NO. 1 and a downstream primer P463-R2 as shown in SEQ ID NO. 2. According to the present invention, the plant growth-promoting bacterium specific primer pair is used for identifying the plant growth-promoting bacterium Penicillium sp.PQxj3, such that the survival state of the Penicillium sp.PQxj3 in the soil can be monitored in real time, the application conditions of the Penicillium sp.PQxj3 in different environments, different crops and different soils can be optimized, and the optimal effect of plant growth promotion can be achieved.

The present invention relates to the technical field of the environmental microbiology, especially to culture medium for high-throughput separation of rhizobacteria from crops, preparation method and application thereof. The culture medium for high-throughput separation of crop rhizobacteria comprises a 1 / l0 TSB medium and a culture medium additive (solution), wherein culture medium additive (solution) is obtained by mixing the crop plants with TSB medium and then by crushing, fermenting, filtering as well as sterilizing. By utilizing the interaction network between multiple microorganisms, crop-source nutrients are fermented using microbiome in-situ in the rhizosphere, and the fermented products are used as carbon and nitrogen source for separation of rhizosphere microorganisms, which can simulate the in-situ environment of rhizosphere microorganisms to the maximum extent, thereby obtaining more microbial species. Combined with the high-throughput separation method, culture medium provided by present invention can obtain more than 60% of bacterial species obtained by high-throughput sequencing.

To understand the growth-promoting and disease-inhibiting activities of plant growth-promoting rhizobacteria (PGPR) strains, the genomes of 12 Bacillus subtilis group strains with PGPR activity were sequenced and analyzed. These B. subtilis strains exhibited high genomic diversity, whereas the genomes of B. amyloliquefaciens strains (a member of the B. subtilis group) are highly conserved. A pairwise BLASTp matrix revealed that gene family similarity among Bacillus genomes ranges from 32-90%, with 2,839 genes within the core genome of B. amyloliquefaciens subsp. plantarum (now B. velezensis). Comparative genomic analyses of B. amyloliquefaciens strains identified genes that are linked with biological control and colonization of roots and / or leaves, including 73 genes uniquely associated with subsp. plantarum (now B. velezensis) strains that have predicted functions related to signaling, transportation, secondary metabolite production, and carbon source utilization. Although B. amyloliquefaciens (now B. velezensis) strains contain gene clusters that encode many different secondary metabolites, only polyketide biosynthetic clusters that encode difficidin and macrolactin are conserved within this subspecies. To evaluate their role in plant pathogen biocontrol, genes involved in secondary metabolite biosynthesis were deleted in B. amyloliquefaciens (now B. velezensis) strain, revealing that difficidin expression is critical in reducing the severity of disease, caused by Xanthomonas axonopodis pv. vesicatoria in tomato plants. This Example defines genomic features of PGPR strains and links them with biocontrol activity and with host colonization.

The present invention provides for a plant-growth-promoting rhizobacterium (PGPR) genetically modified such that the PGPR is capable of colonizing the root of a plurality of plant species, such as a plurality of crop plant species, wherein the genetically modified PGPR is enhanced in the capability to colonize the root of a plurality of plant species when compared to a wild-type or unmodified PGPR.

The present application relates to the field of microbial technology, and specifically provides a plant growth promoting rhizobacteria NFB10 and its application. The strain is isolated from the rhizosphere soil of rubber tree in Hainan province, and is a potential new species of Pseudomonas. The similarity of 16S rDNA sequence of the strain to similar strains is higher than 99.8%, but the average nucleotide identity of whole genome between species is lower than the inter-species threshold of 95%. The strain has the abilities of phosphorus solubilization, potassium solubilization and nitrogen fixation, and can produce indole acetic acid, cellulase, amylase and ammonia and other growth promoting substances. The strain grows well in the salt concentration of 3% or less and the pH range of 5.0-9.0. Experiments show that the strain can significantly improve the germination rate, root length and sprout length of wheat seeds, and has obvious promoting effects on the plant height, ground diameter, biomass and chlorophyll content of the leaves of the seedlings of Hevea brasiliensis. The strain can be used for preparing plant growth promoting bacteria agents, and is suitable for green cultivation and yield improvement of rubber trees and other crops.

The present invention relates to a new Bacillus velezensis strain with deposit number CECT 30599 as well as uses thereof in agriculture. Specifically, the present invention relates to the use of the B. velezensis strain CECT 30599 to improve the resistance of a plant to drought, promote germination in a plant, promote the growth of a plant, the control of pathogenic microorganisms in a plant, reduce the water supply to a plant, promote flowering in a plant, and / or increase the population of plant growth-promoting rhizobacteria in the soil where a plant is grown.