36 results about "Bradyrhizobium japonicum" patented technology

Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells are Bradyrhizobium japonicum cells having the identifying characteristics of B. japonicum strain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.

Lipo Chitooligosaccharide (LCO) [NodBj-V(C18:1,Mefuc)] isolated from Bradyrhizobium japonicum strain 532C was able to stimulate seed germination/seedling emergence, or in the case of potato, sprouting, of a number of crop plants representing eight distantly related plant families (Poaceae, Fabaceae, Brassicaceae, Cucurbitaceac, Malvaceae, Asteraceae, Chenopodiaceae and Solanaceae) of plants, at 25 and/or at 15° C. It also promoted sprouting potato minitubers. Other LCOs [NodRM-V(C_16:2,5) and LCO from R. leguminosarum] were also shown to also display growth-promoting effects on the tested crop plants. The compositions comprising at least one LCO are shown to be effective in promoting growth under both laboratory and field conditions. The invention thus also relates to methods for promoting seed germination and/or seedling emergence and/or growth of plants comprising subjecting the seeds and/or plants to an effective amount of an agricultural composition comprising at least one LCO.

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

The invention relates to bradyrhizobium japonicum capable of effectively fixing nitrogen and a culture method and application thereof. The bradyrhizobium japonicum CGMCC No.4346 is cultured by the steps of strain activation, seed culture, fermentation culture and the like. The invention has the following beneficial effects: sorbitol and diethylene glycol are utilized to replace the carbon source, mannitol in the traditional YEM (yeast extract mannitol) culture medium, and when the improved fermentation culture medium is adopted for culturing, the viable count of the fermentation liquor is higher than that of the YEM culture medium; compared with the rhizobium inoculants which are not inoculated on the soy beans, the bradyrhizobium japonicum agent ensures the theoretical yield of the soy beans to be increased by 16.7-17.2%, the crude fat content to be increased by 0.65-1.21% and the protein content to be increased by 1.29-1.60% after being inoculated on the soy beans, thus being an excellent strain for production of rhizobium japonicum and inoculation of the soy beans; and the culture method is simple and practical, and the raw materials have wide sources and low price and are suitable for large-scale industrialized production.

The subject invention relates to compounds and compositions which induce transcripts of the nolA gene in nitrogen-fixing bacteria, such as Bradyrhizobium japonicum. Novel bacterial strains which are insensitive too NolA, soil inoculants comprising NolA insensitive bacteria and/or nolA inducers, and methods of increasing nitrogen fixation in legumes are also disclosed.

The invention provides a (+)gamma-lactamase with racemate gamma-lactam resolution activity and application of its coding gene in chiral compounds, as well as a method for production of (-)gamma-lactam with bradyrhizobium japonicum. The method comprises: extracting the genomic DNA of the bradyrhizobium japonicum, constructing a recombinant thallus for recombinant protein extraction, then conducting expression to obtain the (+)gamma-lactamase; and adding a gamma-lactam substrate into a phosphate buffer solution in which the recombinant thallus for recombinant protein extraction or the (+)gamma-lactamase suspends, and then carrying out reaction and extraction so as to obtain the (-)gamma-lactam. Or the method can be performed as the following: extracting the genomic DNA of the bradyrhizobium japonicum, constructing the recombinant thallus for recombinant protein extraction, then conducting expression to obtain the (+)gamma-lactamase, adding the substrate, and implementing reaction and extraction, thus obtaining the (-)gamma-lactam. The (+)gamma-lactamase provided in the invention derives from the bradyrhizobium japonicum and has high catalytic efficiency. The obtained (-)gamma-lactam has high purity.

The invention discloses a heat-resistant recombinant nitrile hydratase gene, an encoded enzyme, a vector, an engineering bacterium and an application of the gene engineering bacterium to catalysis of a nitrile compound to produce amide and a biodegradation herbicide, that is, dichlobenil. The functional expression of the tentative nitrile hydratase gene from bradyrhizobium japonicum USDA 110 is realized, and the crucial role of an activation element of the nitrile hydratase in the expression process is proved. Meanwhile, the invention provides a method for constructing the nitrile hydratase gene engineering bacterium in Escherichia coli, and the nitrile hydratase with the high expression quantity and the high activity is obtained from the nitrile hydratase gene with the participation of the activation element.

The present disclosure relates to methods of rational genome mining. A method may include narrowing the number of clones that would otherwise need to be screened and/or identifying a gene with a desired catalytic activity. The disclosure also relates to a nitrile hydrolase from Bradyrhizobium japonicum USDA110 first identified by rational genome mining. In addition, the disclosure relates to nitrilase bll6402 and catalytically active variants capable of converting an α-hydroxy nitriles, a β-hydroxy nitrile and/or an α,ω-dinitrile to a carboxylic acid.

The invention discloses bradyrhizobium japonicum vitrified cryopreservation liquid and a method for vitrified cryopreservation of bradyrhizobium japonicum and relates to microorganism vitrified cryopreservation liquid and a method for vitrified cryopreservation of microorganisms. The bradyrhizobium japonicum vitrified cryopreservation liquid contains ethanediol and is simple in compound composition. The bradyrhizobium japonicum vitrified cryopreservation liquid is composed of glycerinum, ethanediol, DMSO and distilled water. According to the method for vitrified cryopreservation of the bradyrhizobium japonicum, bradyrhizobium japonicum liquid is added into the bradyrhizobium japonicum vitrified cryopreservation liquid, and then the bradyrhizobium japonicum vitrified cryopreservation liquid is put into liquid nitrogen for fast vitrified cryopreservation. The bradyrhizobium japonicum vitrified cryopreservation liquid and the method for vitrified cryopreservation of the bradyrhizobium japonicum are used in the field of microorganism preservation.

The invention discloses gamma-lactamase with racemate gamma-lactam resolution activity as well as an encoding gene and applications thereof, namely, bradyrhizobium japonicum (Bradyrhizobium japonicum USDA6) derived gamma-lactamase as well as an encoding gene and applications thereof, and provides an application of the gamma-lactamase in the hydrolytic resolution of racemate gamma-lactam. The gamma-lactamase disclosed by the invention is a protein as the following (a) or (b): (a) a protein consisting of amino acid residue sequences shown in SEQ ID No:1 in a sequence table; and (b) a protein formed by that amino acid residue sequences shown in SEQ ID No:1 in a sequence table are replaced and/or deleted and/or added by one or more amino acid residues, with racemate gamma-lactam resolution activity, and derived from SEQ ID No:1. Racemate gamma-lactam is subjected to hydrolytic resolution by using the gamma-lactamase, the resolution activity of the gamma-lactamase is close to 100%, and then gamma-lactam is prepared by using a catalytic product amino acid.

The invention discloses a method of researching functions of genes of Bradyrhizobium japonicum USDA110, and provides a prokaryotic expression system which utilizes pseudomonas aeruginosa as host bacteria; the pseudomonas aeruginosa is Pseudomonas aeruginosa PAO1. In the invention, phenotype research related to gene synthesis and degradation functions of intracellular messenger cyclic di-guanosinemonophosphate (c-di-GMP) in the Bradyrhizobium japonicum is directly carried out in the pseudomonas aeruginosa of which the background mechanism research is definite; by observing the character changes of the pseudomonas aeruginosa, functions of the target gene is initially obtained. The invention provides the novel prokaryotic expression system in which the gene of the Bradyrhizobium japonicum isexpressed and colonial morphology, moveability, generation of exopolysaccharide and generation quantity of biomembrane are inspected, thereby initially obtaining the function of the gene of the Bradyrhizobium japonicum. The method saves time and consumable costs, accelerates researching process and is beneficial to research on the gene function of Bradyrhizobium japonicum.

The invention discloses a nitrogen-fixing bradyrhizobium japonicum strain resistant to saline-alkali soil and application of the bradyrhizobium japonicum strain. According to the invention, a strain of bradyrhizobium taihuae Dian strain is obtained through separation and is preserved in Guangdong Microbial Culture Collection Center on January 11, 2022, and the preservation number is GDMCC No: 62202. Researches show that the strain has the characteristics of high nodulation rate and strong nitrogen fixation capability, and can optimize the micro-ecological environment of alkaline soil crop root growth; the Dian strain is adopted to treat plants, soybean nodulation and nitrogen fixation can be effectively promoted, plant nitrogen nutrition is increased, plant growth is promoted, and the biomass, root nodule number and nitrogen content of soybean plants can be remarkably improved. The strain is especially suitable for cultivation and production of soybeans in stony desertification areas, expands a strain resource library of nitrogen-fixing rhizobium, is beneficial to promoting nodulation and nitrogen fixation of plants, and increases nitrogen nutrition of the plants.

The invention discloses a D-lactate dehydrogenase gene b116812S derived from a high-efficiency nitrogen fixation bradyrhizobium japonicum as well as a preparation method and application of the D-lactate dehydrogenase gene b116812S. A nucleotide sequence of the D-lactate dehydrogenase gene b116812S is shown in SEQ ID No.1, and an amino acid sequence coded by the D-lactate dehydrogenase gene b116812S is shown in SEQ ID No.2. The D-lactate dehydrogenase gene b116812S is prepared by reconstructing a D-lactate dehydrogenase gene b116812 derived from the high-efficiency nitrogen fixation bradyrhizobium japonicum according to a plant preferred codon and by using a gene synthesis method. The D-lactate dehydrogenase gene b116812S is converted into arabidopsis thaliana to obtain a transgenosis arabidopsis thaliana, and the performance of the transgenosis arabidopsis thaliana resisting glyphosate is remarkably superior to that of wild type arabidopsis thaliana, and can be applied to glyphosate-resisting crop breeding.

A biological inoculant having both fertilizing and fungicidal activity is disclosed. More particularly, a bio-inoculant having the said combined effects, comprising Bradyrhizobium japonicum and specific isolates from the Trichoderma genus is disclosed. The bio-inoculant is applied to soybean crops for preventing fungi borne diseases. More particularly, the bio-inoculant of the invention is useful for protecting soybean crops against infection by Fusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp. and Rhizoctonia sp.

The invention relates to an environment-friendly biological agent for treating fruit and vegetable organic garbage. The microbial agent comprises 4-8 parts of white rot fungus powder, 3-6 parts of streptomyces powder, 6-9 parts of bacillus licheniformis powder, 6-9 parts of bacillus pumilus powder, 4-8 parts of nitrobacteria powder, 8-10 parts of lactic acid bacteria powder, 7-10 parts of bacillus megatherium powder, 8-12 parts of bacillus subtilis powder, 8-12 parts of bacillus amyloliquefaciens powder and 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of Bradyrhizobium japonicum powder, 4-8 parts of azotobacter chroococcum powder, 3-7 parts of Thermomonospora fusca powder, 1-3 parts of lactobacillus delbrueckii, 1-5 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino-oligosaccharin and 0.05-1 part of brassinolide. The environment-friendly biological agent can be applied to treatment of different fruit and vegetable organic solid wastes, the treatment period is greatly shortened to 24 hours to one week, the process is environmentally friendly and harmless, the final treatment product is the biological bacterial fertilizer, and waste is turned into wealth.

The invention provides a microbial agent suitable for planting of corn in saline-alkali soil. The microbial agent is prepared by the following steps: firstly preparing composite microbial powder fromtrichosporon cutaneum, bradyrhizobium japonicum, trichoderma viride, bacillus coagulans, pseudomonas cepacia, and azotobacter chroococcum, mixing the composite microbial powder and a pre-mixed material prepared from corn straw powder, mushroom residues, cottonseed meal and other materials, performing anaerobic fermentation to obtain a fermented material, pouring the fermented material into a covering material prepared from polyaspartic acid and a polyvinyl alcohol aqueous solution to obtain a pre-prepared material, finally adding polycaprolactone into the pre-prepared material, performing uniform stirring to obtain a mixture, adding activated sludge into the mixture, and performing aerobic fermentation to obtain the the microbial agent. After the microbial agent provided by the invention is applied into the saline-alkali soil, the physical and chemical properties of the soil can be improved, the nutrient requirements of growth of the corn can be met, and the yield of the corn can be improved.

The invention relates to a method for whole-cell splitting of a racemate beta-lactam by using Bradyrhizobium japonicum, and belongs to the technical field of separation. According to the present invention, the whole-cell transformation splitting of 3-hydroxy-4-phenyl-2-azetidinone and 3-acetoxy-4-phenyl-2-azetidinone with Bradyrhizobium japonicum has high catalysis efficiency, and the high purity single configuration compound can be obtained, wherein the (3R,4S)3-hydroxy-4-phenyl-2-azetidinone e.e value is 99.99%, the target product relative yield is 99%, the total yield is 49.5%, the (3R,4S)3-acetoxy-4-phenyl-2-azetidinone e e.e value is 99.88%, the target product relative yield is 93.58%, and the total yield is 46.79%.

The invention discloses a high-density culture method for slow-growing rhizobium japonicum, and belongs to the technical field of heterotrophic culture fermentation processes. The method solves the problems that the carbon nitrogen ratio in the logarithmic phase is unbalanced, the growth of thalli is poor due to the increase of metabolites, and the high-density culture of the slow-growing rhizobium japonicum cannot be realized in the conventional method for culturing the rhizobium japonicum in a fermentation tank. According to the method, a constant-speed fed-batch feeding mode is adopted, the ratio and concentration of carbon and nitrogen in fermentation liquor are effectively maintained, the viable count of the slow-growing rhizobium japonicum in the logarithmic phase is effectively increased, the final effective viable count of fermentation is stabilized at 6.8 * 10 < 9 > cfu/mL, and high-density culture of the rhizobium japonicum is achieved. In addition, the adopted constant-speed fed-batch feeding mode is easy to operate, amplification culture is easier, and the method is more suitable for large-scale production.