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Composite microbial fertilizer and preparation method thereof

A technology of compound microorganisms and fertilizers, applied in the field of compound microbial fertilizers and its preparation, can solve problems such as difficulty in functioning and poor survival, and achieve the effect of increasing the amount of planting and fertilizer effect time, improving preservation performance and functional effect

Inactive Publication Date: 2013-06-12
沈阳科丰牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Difficult to function if microorganisms are poorly survivable after entering the soil

Method used

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  • Composite microbial fertilizer and preparation method thereof
  • Composite microbial fertilizer and preparation method thereof
  • Composite microbial fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The compound microbial fertilizer is composed of a compound microbial fermented liquid and an embedding agent, and the ratio of parts by volume is compound microbial fermented liquid: embedding agent = 1:4.

[0021] Preparation:

[0022] 1) Activate and culture Candida utilis, Streptococcus lactis, Lactobacillus acidophilus and Bacillus subtilis respectively according to conventional methods to the logarithmic growth phase, and then the activated Candida utilis, Streptococcus lactis, and Bacillus subtilis Lactobacillus acidiformis and Bacillus subtilis are mixed according to the volume ratio 1:1:1:1, and then the mixed strains are inoculated in the liquid fermentation medium according to 4% by weight for compound culture, and at 30°C, ferment and cultivate for 70h, and ferment The pH value of the substance is 4.5-7.5, until the total number of bacteria reaches more than 109 / mL; the liquid fermentation medium is 2% glucose, 1% peptone, 1% beef extract, 0.5% yeast extract...

Embodiment 2

[0043] The compound microbial fertilizer is composed of a compound microbial fermented liquid and an embedding agent, and the ratio of parts by volume is compound microbial fermented liquid: embedding agent = 1:5.

[0044] Preparation:

[0045] 1) Activate and culture Candida utilis, Streptococcus lactis, Lactobacillus acidophilus and Bacillus subtilis respectively according to conventional methods to the logarithmic growth phase, and then the activated Candida utilis, Streptococcus lactis, and Bacillus subtilis Lactobacillus acidiformis and Bacillus subtilis are mixed according to the volume ratio 1:1:1:1, and then the mixed strains are inoculated in the liquid fermentation medium according to 4% by weight for compound culture, and at 30°C, ferment and cultivate for 70h, and ferment The pH value of the substance is 4.5-7.5, until the total number of bacteria reaches more than 109 / mL; the liquid fermentation medium is 2% glucose, 1% peptone, 1% beef extract, 0.5% yeast extract...

Embodiment 3

[0054] Rapeseed was used as the test material, and the pot test was used to detect the colonization amount of the compound microbial fertilizer embedded particles in the soil, and the applied amount was 2×1011 bacteria per gram of soil. Rapeseed rhizosphere soil samples were collected at different intervals after application, and the number of microorganisms added to the soil was detected by plate counting method, and the amount of soil colonization as microbial fertilizer is shown in Table 2 for details.

[0055] Table 2 The amount of colonization of compound microbial fertilizer embedded particles in soil

[0056]

[0057] The above operations, culture solution, reagents and strains used are conventional operations, culture solutions, commercially available reagents and strains in the art unless otherwise specified. In addition, the composite microbial fertilizer of the present invention adopts functional microbial fermentation liquid, sodium alginate, gelatin, etc. as ma...

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Abstract

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a compound microbial fertilizer and a preparation method thereof. Background technique [0002] Studies have shown that nitrogen-fixing bacteria, bacillus, photosynthetic bacteria and cellulolytic bacteria, lactic acid bacteria, yeast, actinomycetes, etc. all have functional roles in plant production and can be developed into microbial fertilizers. Microbial fertilizers are completely different from general organic or inorganic fertilizers that directly supply nutrients or drugs. They use functional beneficial microorganisms to exert special functions such as fixing nutrients and disease resistance after being applied to the soil. If the survival of microorganisms after entering the soil is poor, it is difficult to play a role. Therefore, a key issue in the application of microbial fertilizers is to enable the microorganisms applied to the soil to reproduce in large numbers, vigoro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
Inventor 高林白子金贾卿金石王国华高忠武于波刘丽丽赵宪娟顾影吴琼张子丰邵中金吴鹏飞陈德民
Owner 沈阳科丰牧业科技有限公司
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