272 results about "Streptococcus" patented technology

The invention relates to a preparation method of a rice leavening with a scalp care effect and a product and application thereof, the preparation method comprises the following steps: mixing a rice raw material, a plant raw material, water, a magnesium salt, a sodium salt and Tween, sterilizing to obtain a fermentation medium, inoculating a compound microbial agent into the fermentation medium, and fermenting to obtain the rice leavening with the scalp care effect. After the fermentation liquid is finished, sterilizing and filtering to obtain a rice leavening; wherein the compound microbial agent comprises a first microbial strain and a second microbial strain; the strains in the first microbial strain comprise a combination of streptococcus thermophilus, bifidobacterium breve and a plant lactobacillus YK-L002 strain; and the second microbial strain comprises a trichosporon YK-S004 strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of four bacteria has excellent hair care and nourishing effects, and can effectively condition the scalp micro-ecological environment and nourish the hair quality.

The invention belongs to the technical field of microorganisms, and relates to a strain of herbaspirillum as well as a fermentation method and application thereof. The invention provides a strain of brassica juncea, the brassica juncea is brassica juncea YLHb25.7, the brassica juncea is named as Herbaspiril huttiense, the brassica juncea is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35297. The invention also provides a preparation method of the brassica juncea. The herbispirillum sp. YLHb25.7 disclosed by the invention has the capabilities of producing ACC deaminase, producing IAA and fixing nitrogen, and has good drought resistance and saline-alkaline tolerance. The herbaspirillum sp. YLHb25.7 and the fermentation product thereof can promote the growth of wheat and corn, can be used as a microbial agent or bacterial fertilizer, and can be applied to the agricultural market.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and / or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 have a synergistic interaction effect, so that the relieving effect on enteritis can be remarkably improved.

The invention belongs to the technical field of bacterium detection, and discloses a multiple PCR primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application thereof. On the basis of a Luminex liquid phase chip technology, specific primers are designed aiming at eight common respiratory tract infection pathogenic bacteria including pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, stenotrophomonas maltophilia, haemophilus influenzae and escherichia coli; the multiple PCR primers which are high in amplification efficiency, good in specificity and suitable for simultaneously detecting the eight respiratory tract pathogenic bacteria are screened out, the multiple PCR system and microsphere hybridization conditions are continuously optimized, the multiple PCR method for the eight respiratory tract pathogenic bacteria is successfully constructed by applying the Luminex xTAG technology, and the multiple PCR method can be applied to rapid detection of multiple pathogenic microorganisms.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for intervening in non-alcoholic fatty liver and a preparation method thereof. The preparation method comprises the following steps: (1) weighing the following raw materials: hawthorn, salvia miltiorrhiza, rhizoma alismatis, radix puerariae, astragalus membranaceus, semen cassiae and poria cocos, and respectively crushing; (2) mixing radix astragali, semen cassiae and poria cocos, and performing water extraction to obtain concentrated paste; (3) preparing a fermentation substrate; (4) simultaneously inoculating lactobacillus rhamnosus and lactobacillus acidophilus into the fermentation substrate, and carrying out first-stage anaerobic fermentation; (5) continuously inoculating lactobacillus paracasei and streptococcus thermophilus into the fermentation liquid, and carrying out second-stage anaerobic fermentation; and (6) carrying out vacuum concentration, freeze drying and crushing on the traditional Chinese medicine fermentation liquor to obtain the traditional Chinese medicine fermentation composition for intervening in the non-alcoholic fatty liver disease. The traditional Chinese medicine composition is prepared through specific raw material compatibility and a specific fermentation mode, and a new path is provided for application of traditional Chinese medicine in the field of fatty liver treatment.

This invention discloses a raw material composition for preparing yogurt, yogurt, food, and a method for preparing the same. The raw material composition includes fermentation ingredients, additives, and a starter culture. The fermentation ingredients include 80-90 wt% soy milk; the additives include 0.1-0.4 wt% dietary fiber, 1-3 wt% natural starch, and 0.5-1.5 wt% coconut extract; the starter culture is used at 0.005-0.02 wt%, and includes *Streptococcus thermophilus*, *Lactobacillus delbrueckii* subsp. bulgaricus, *Lactobacillus casei*, and *Lactobacillus plantarum*. The yogurt of this invention has a good taste and flavor at room temperature, maintains a stable pH, and can be stored at room temperature for one month while ensuring the activity of lactic acid bacteria.

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

The application discloses a kind of fermentation complex microbial inoculant and preparation method and application thereof, the fermentation complex microbial inoculant includes the following weight parts of component: bacillus subtilis microbial inoculant 2-5 parts, bacillus mucilaginosus microbial inoculant 5-10 parts, yeast microbial inoculant 2-10 parts, trichoderma harzianum microbial inoculant 10-20 parts, aspergillus niger microbial inoculant 10-20 parts and streptococcus thermophilus microbial inoculant 2-5 parts;The fermentation complex microbial inoculant of the application is prepared by selecting each strain and amount, is added to pigeon manure and is fermented to prepare organic fertilizer, can better decompose macromolecular substance, significantly improve the organic matter and total nitrogen content in preparation organic fertilizer, and can better improve seed germination rate;In addition, the preparation method of pigeon manure organic fertilizer of the application is simple in process, easy to operate, and convenient for production.

The application discloses a silver / schiff base antibacterial material, a preparation method and application, and relates to the field of antibacterial materials.The silver / schiff base antibacterial material, the preparation method and the application are characterized in that, based on the large electron cloud density of the carbon-nitrogen double bond of the schiff base, silver ions in a silver nitrate solution are uniformly adsorbed on a schiff base base through the electrostatic adsorption principle; under the reduction of a reducing agent, the silver ions are reduced to nanosilver ions, and a silver / schiff base antibacterial material is obtained, in which nanosilver ions in the form of nanospheres are uniformly dispersed on the schiff base in the form of a thin sheet layer, thereby greatly improving the dispersibility of the nanosilver ions.The preparation method is simple, low in cost, and capable of batch production, and has no cytotoxicity, and has high antibacterial effects on streptococcus, pseudomonas aeruginosa and staphylococcus aureus, and has a good application prospect.

The invention belongs to the field of food processing and fermentation engineering, and discloses a multi-strain composite leavening agent with multiple application characteristics and application of the multi-strain composite leavening agent in preparation of fermented milk. The fermentation agent is prepared from lactobacillus bulgaricus IMAU20428, lactobacillus bulgaricus IMAU20427, streptococcus thermophilus IMAU20229 and streptococcus thermophilus IMAU10630. Systematic research finds that different compounding proportions can significantly influence the fermentation time, post-acidification phenomenon, viscosity, water-holding capacity, texture characteristics, amino acid and organic acid content and metabolic pathway enrichment characteristics of the fermented milk. The composite leavening agent provided by the invention can realize differentiated development of fermented milk products through proportion regulation and control, and provides technical support for innovation of the dairy product industry.

The invention belongs to the technical field of microorganisms, a streptococcus zooepidemicus mutant strain CGMCC (China General Microbiological Culture Collection Center) No.26991 is screened out, the streptococcus zooepidemicus mutant strain is obtained through screening by adopting a phage secondary infection method, resistance experiments and genetic stability experiments prove that the screened strain is an anti-phage strain, the resistance can be stably inherited, and the strain can be used as a high-yield streptococcus zooepidemicus mutant strain. The strain not only has good resistance to bacteriophages, but also has excellent high-yield hyaluronic acid characteristics, and can be used as an industrial fermentation strain of hyaluronic acid.

A general formula for lysins based on which the activity and functionality are further screened and 24 lysins are identified and further investigated, nine of which are characterized. These lysins exhibit potent bactericidal activity, capable of lysing multiple species of Staphylococcus, Streptococcus, and / or Enterococcus; demonstrate robust environmental resistance and stability, retaining activity under varying temperatures, NaCl concentrations, pH levels, and in immune serum; and effectively treat localized and systemic infections. These lysins and their variants are promising as antimicrobial agents for eradicating bacteria, preventing bacterial infections, or treating bacterial infections (caused by genera Staphylococcus, Streptococcus, and / or Enterococcus).

The invention belongs to the technical field of traditional Chinese medicines, and relates to a method for improving the sensitivity of streptococcus mutans to a glycyrrhiza uralensis extract. According to the method, the glycyrrhiza uralensis extract and hydrogen ions jointly act on streptococcus mutans in an acid environment. Research finds that the inhibitory activity of part of the effective components of the glycyrrhiza uralensis extract on streptococcus mutans in an acid environment is remarkably enhanced. The glycyrrhiza uralensis extract and hydrogen ions have a synergistic effect, and the acidic environment can improve the sensitivity of the isostreptococcus to the glycyrrhiza uralensis extract and enhance the bactericidal effect.

The application discloses a digital image quantitative dual-mode detection system and method based on a cascade enhanced nano-enzyme, which comprises a probe prepared from a membrane nano-enzyme material, an immunochromatography test strip and a signal automatic analysis system, the nano-enzyme material takes molybdenum disulfide nanosheets as a substrate, sequentially loads large-diameter platinum-iridium alloy particles and small-diameter platinum-iridium alloy particles through polyethylene imine-mediated electrostatic self-assembly, and forms a high-density catalytic active site array. The probe is coupled with specific antibodies on the surface of the nano-enzyme material. Based on the inherent light absorption and peroxidase-like activity of the probe, a dual-mode detection strategy of physical color development-catalytic enhancement is established, high-concentration sample visual preliminary screening and low-concentration sample ultra-sensitive quantitative detection are realized, and combined with an adaptive cross-peak colorimetric quantitative algorithm, individual visual perception differences can be effectively eliminated, and on-site rapid quantitative screening of community-acquired pneumonia pathogens such as streptococcus pneumoniae and legionella pneumophila can be realized.

The application provides a quality control for detecting respiratory pathogen nucleic acid, which comprises any one or more of the following respiratory pathogens: coronavirus, influenza virus, adenovirus, Mycoplasma pneumoniae, Streptococcus pneumoniae, rhinovirus or / and Legionella pneumophila. The full-length genome sequence of the coronavirus is divided into 6 target fragments, each with a length of 4000-5500 bp. The quality control of the application covers all detection target sequences (or target points) of the coronavirus, has a wide coverage, comprehensively detects target points, and does not have the phenomenon of missed detection. The quality control of the application contains main respiratory pathogen infections, has a wide range, and uses real virus samples and lentivirus samples as raw materials, and has more accurate detection target points. The preparation of the coronavirus quality control of the application integrates the target gene sequence into the host genome through the lentivirus vector, knocks out the self-replication gene in the preparation process, and has a "self-inactivation" ability, so that the recombinant lentivirus cannot replicate in the target cells and infect other cells.

The application relates to the field of food, in particular to zero-cane normal-temperature yoghourt and a preparation method thereof. The zero-cane normal-temperature yoghourt comprises the following raw materials in parts by weight: raw cow milk or concentrated milk 80-90 parts, protein powder 0.4-3 parts, cheese 0.3-2 parts, sweetening agent 0.01-15 parts, stabilizer 0.1-5 parts and bacterial strain 50-200 U, the bacterial strain is composed of lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus and lactobacillus bifidus, wherein the sum of lactobacillus bulgaricus and streptococcus thermophilus: the sum of lactobacillus acidophilus and lactobacillus bifidus = 9:1-8:2. The application adopts raw cow milk or concentrated milk, protein powder and cheese to form a basic system in a specific proportion, and is fermented by specific bacterial strains, so that the zero-cane yoghourt can reach a fermentation end point within 8 hours, and the product has a good taste.

The invention discloses a private care wet tissue containing a bacteriostatic composition, the private care wet tissue comprises a composite non-woven fabric base material and an impregnation liquid, the impregnation liquid comprises the bacteriostatic composition, a soothing anti-inflammatory agent, a moisturizing repairing agent, an odor removal regulator, a cosolvent and a stabilizer; the preparation method comprises the following steps: adding purified water, a citric acid-sodium citrate buffer agent and EDTA (Ethylene Diamine Tetraacetic Acid) disodium into a vacuum stirring tank, then adding glycerol, micromolecular sodium hyaluronate and ceramide 3, and then adding L-lactic acid and a lactobacillus acidophilus fermentation product; sequentially adding nisin, cranberry extract, polyhexamethylene biguanide hydrochloride and beta-cyclodextrin inclusion tea tree essential oil, and finally adding dipotassium glycyrrhizinate, allantoin and citrus extract. Different from the prior art that private care wet tissues only have a single antibacterial function and neglect flora balance, peculiar smell is covered by depending on essence, the base material and the steeping liquor are designed at the same time, and the functions of bacteriostasis and antibiosis, gentle private care, odor removal and itching relieving, inflammation prevention and swelling prevention, flora balance maintenance and the like are achieved.

This invention aims to address the technical bottlenecks of existing milk curd products, such as weak functional activity, unclear process parameters, and unstable flavor and quality. It utilizes *Pediococcus pentosaceus* HM4, *Streptococcus thermophilus* HM6, *Lactococcus lactis* JY4, and *Lactobacillus plantarum* NC1, employing these four strains in an optimal ratio as a compound fermentation agent to achieve synergistic effects. Using milk and goji berries as raw materials, the fermentation process of milk curds and goji berry milk curds was systematically optimized through single-factor experiments, Plackett-Burman experiments, and response surface methodology. The physicochemical indicators and flavor characteristics of the products under different process conditions were analyzed. The obtained milk curds achieved a DPPH free radical scavenging rate of 91.38%–94.15%, providing technical support for the high-value utilization of milk curds and enriching the variety of functional dairy products.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

The invention relates to an application of a composition of seven probiotics in preparation of an anti-influenza virus probiotic agent. The bacterial strains in the probiotic composition comprise a combination of a streptococcus thermophilus CT-02 bacterial strain, a bifidobacterium animalis subsp. Lactis C-2 bacterial strain, a bifidobacterium longum subsp. Infantis C-3 bacterial strain, a lactobacillus delbrueckii subsp. Bulgaricus CB-01 bacterial strain, a lactobacillus acidophilus CL-01 bacterial strain, a lactobacillus paracasei CL-02 bacterial strain and a lactobacillus rhamnosus CL-03 bacterial strain. New application of the probiotic composition is developed, a research basis is provided for wide application of the probiotic composition, meanwhile, a brand-new strategy for preventing, relieving or treating influenza is also developed, it is found that the probiotic composition has a very remarkable effect in the aspect of relieving related symptoms caused by the influenza, and the probiotic composition has a good application prospect. Specifically, related symptoms such as respiratory system infection, body temperature imbalance, weight loss and vision weakening caused by influenza virus infection are obviously improved.

The present application relates to the technical field of fermented milk, and particularly relates to a normal-temperature zero-sucrose yoghurt and a preparation method thereof.The preparation method of the normal-temperature zero-sucrose yoghurt provided by the present application comprises the following steps: preparing raw cow milk, and adding stabilizers, sweeteners, inulin or xylo-oligosaccharides, whey proteins and honeysuckle extract liquid, and then performing fermentation by using streptococcus thermophilus, lactobacillus bulgaricus and probiotics as fermentation strains.The normal-temperature yoghurt provided by the present application can ensure product flavor and shelf life stability and achieve the effect of weight loss through the synergistic effect of various raw materials.It is verified through animal experiments that the normal-temperature zero-sucrose yoghurt prepared by the present application has the effects of regulating rat weight, body fat rate and weight-related genes during the shelf life.

In some embodiments, a composition comprising Bifidobacterium bifidum, Bifidobacterium longum, Lactobacillus plantarum, and Streptococcus thermophilus is provided. In some embodiments, provided is the use of a composition of any of the examples herein for preventing or alleviating anxiety and / or sensory hypersensitivity, preventing or treating autism spectrum disorder (ASD), anxiety disorder and / or gastrointestinal (GI) disorder or condition, enhancing attention and / or concentration, or stimulating a GABA receptor in a subject. In some embodiments, methods and kits are provided for preventing or alleviating anxiety and / or sensory hypersensitivity, preventing or treating autism spectrum disorders, anxiety disorders, and / or gastrointestinal disorders or conditions, enhancing attention and / or concentration, or stimulating GABA receptors in a subject. In certain embodiments, the compositions are safe to use and effective to prevent or alleviate anxiety and / or sensory hypersensitivity in subjects, particularly children subjects.

The application discloses a complex preparation containing bovine lactoferrin and lyticase and application thereof, and belongs to the technical field of biology. The preparation is composed of bovine lactoferrin and streptococcal lyticase, and both are prepared through a baculovirus-insect cell eukaryotic expression system. Researches show that the complex preparation has a significant synergistic antibacterial effect. The complex preparation not only can effectively inhibit a plurality of gram-positive bacteria, but also can overcome the limitation that single streptococcal lyticase has weak activity on gram-negative bacteria, and significantly enhances the inhibiting effect on escherichia coli and salmonella. Especially, the complex preparation still has clear in-vitro antibacterial activity on multi-drug resistant escherichia coli such as ciprofloxacin and doxycycline, thereby providing a new way for developing a new type of drug for resisting drug-resistant bacteria.

A composition of oral administration for use in the prevention or and treatment degenerative diseases of the eye that it comprises lysates of probiotic micro-organisms in the form of a dry powder of the genus Bacillus, Lactobacillus, Streptococcus, Saccharomyces and Bifidobacterium.

The application belongs to the technical field of enzymology or microbiology devices, and particularly relates to a fermentation device for dry choking yogurt production, which comprises a fermentation tank, a connecting shell is fixed at the top end of the fermentation tank, a speed reducer motor is installed at the top end of the connecting shell, a feeding port is fixed at one side of the top end of the fermentation tank, an inner container is arranged in the fermentation tank, a plurality of micropores are arranged at equal intervals on the outer side of the inner container, a liquid discharge pipe is fixed at one side of the bottom end of the fermentation tank, and a discharge port is arranged at the bottom end of the fermentation tank; a stirring rod is arranged in the inner container, a plurality of connecting blocks are fixed to the outer side of the stirring rod, and a plurality of blades are rotatably connected to the outer side of the connecting blocks; the base material, streptococcus thermophilus and lactobacillus bulgaricus are fully mixed through the blades, lactobacillus bulgaricus secretes protease to perform enzymolysis on casein to form a gel network, the blade structure is matched with the lifting adjusting assembly, bidirectional material stirring of the base material can be realized without changing the stirring driving motor, and the mixing uniformity of the base material and the strains is improved.

The present application relates to a kind of preparation method of barley and gastrodia elata beverage, belong to food processing technical field.The existing gastrodia elata beverage preparation method has the problems such as low active ingredient extraction efficiency, difficult to synergistically utilize barley functional components, flavor relies on exogenous addition, product is easy to precipitate etc..The present application is by the specific proportion of mixing maturation of barley and gastrodia elata, inoculation includes saccharomyces cerevisiae, lactobacillus plantarum and streptococcus thermophilus Compound starter carries out solid state fermentation, then it is by the compound enzyme preparation including alpha-amylase, beta-glucanase and pectinase enzymolysis and subsequent processing, and beverage is prepared.The method synergistically improves gastrodia elata element and barley beta-glucan enrichment, creates coordinated flavor, and improves product stability.The obtained beverage is mainly used to provide the flavor beverage with the nutritional and health care function of gastrodia elata and barley.

The invention relates to a polynucleotide comprising a lacZ gene (lacZFS) encoding a β-galactosidase characterized by a particular profile with respect to its lactose hydrolysis efficiency. The invention also relates to a strain of Streptococcus thermophilus comprising a lacZFS allele and a bacterial composition thereof, and its use for obtaining fermented milk that does not undergo subsequent acidification.

The invention provides a staphylococcus aureus nanocrystallization antigen, a vaccine containing the staphylococcus aureus nanocrystallization antigen and application of the staphylococcus aureus nanocrystallization antigen. The staphylococcus aureus nanocrystallization antigen is obtained by modifying a staphylococcus aureus antigen through a nanocrystallization matrix, and the nanocrystallization matrix is one or more of streptococcus pneumoniae capsular polysaccharide, staphylococcus aureus capsular polysaccharide and sodium hyaluronate; the staphylococcus aureus antigen is one or more of staphylococcus aureus enterotoxin A, staphylococcus aureus enterotoxin B, staphylococcus aureus enterotoxin C2 and toxic shock toxin syndrome toxin 1. The staphylococcus aureus nanocrystallization antigen and the vaccine containing the staphylococcus aureus nanocrystallization antigen can induce high-level immune response, the antibody durability is high, the dosage of the used antigen is low, and the safety is good; and the preparation method is simple, high in production efficiency and low in cost.