Compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof

A compound microbial agent and pesticide residue technology, which is applied in the fields of biotechnology and environmental protection, can solve the problems of high incidence of pests and diseases, long cycle, and inability to solve the pollution of pesticide and antibiotic residues, etc.

Inactive Publication Date: 2017-02-22
中山市润泽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the existing aquaculture manure treatment technology, there are two ways to deal with livestock and poultry manure and excrement: the first is natural composting and fermentation to form compost. The disadvantages of this method are long cycle, serious environmental pollution and fertilizer efficiency. Low, easy to form secondary pollution; the second method is to inoculate the microbial starter into the excrement, ferment and decompose it to make organic fertilizer
Although the second treatment method can achieve organic matter returning to the field through decomposing and deodorizing, the antibiotics and other chemicals remaining in the organic matter have not been decomposed. The organic fertilizer obtained in this way has not been degraded after being applied to the farmland. Chemical drug residues will still destroy the balance of beneficial microbial colonies in the soil, weakening the ability of plants to resist pests and diseases, and are potential threats and pollution in the agricultural ecological environment
These two types of compound microbial agents only act on the fermentation and decomposing function of organic matter, microbial nitrogen fixation, phosphorus dissolution, potassium dissolution and partial biological control effects, and are effective on chemical drugs and antibiotic residues in livestock and poultry manure and agricultural ecological environment. Pesticide residues in the can not be degraded
According to the existing preparation technology and product efficacy of agricultural microbial agents, there are obvious technical defects, that is, it cannot solve the residual pollution of pesticides and antibiotics in the agricultural ecological environment
At present, the long-term excessive use of chemical fertilizers and pesticides has caused the imbalance of soil beneficial microbial communities, soil compaction, weakened soil sustainable fertilizer supply capacity, high incidence of diseases and insect pests, and the carrying capacity of the agricultural ecological environment has reached its limit. Therefore, ecological restoration and construction of new ecological balance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Embodiment 1 Adopt the following steps to prepare composite microbial inoculum of the present invention:

[0153] 1. Ten strains of Bacillus subtilis, Bacillus licheniformis, Aspergillus niger, Bacillus colioids, Thermococcus, Enterococcus faecalis, Lactobacillus plantarum, Bacillus polymyxa, Bacillus megaterium and Pseudomonas fluorescens were tested separately Screening of strains and optimization of culture conditions to obtain purified strains; high-density culture of purified strains, inoculation into shake flasks for activation culture, inoculation of activated cultured strains into fermenters for expansion culture; after expansion culture, the The fermented bacterial liquid of the single strain obtained by expanding the culture respectively is dehydrated and dried to obtain the dry powder of the above-mentioned 10 bacterial strains; the specific steps are as follows:

[0154] 1. Prepare the dry powder of Bacillus subtilis by the Bacillus subtilis strain according...

Embodiment 2

[0265] Embodiment 2 According to the percentage by weight of the number of live bacteria in the dry powder of various strains of bacterial cells accounting for the total number of live bacteria in the composite bacteria powder: Bacillus subtilis 15%, Aspergillus niger 15%, Bacillus colloidus 15%, Enterococcus faecalis 15%, lichen Bacillus 8%, Bacillus megaterium 8%, Pseudomonas fluorescens 8%, Lactobacillus plantarum 5%, Bacillus polymyxa 5%, Streptococcus thermophilus 6%. The dry bacterial powders of 10 kinds of purified bacterial strains prepared in the first step of the embodiment were mixed evenly, and the composite bacterial powder with multiple bacterial cells was injected into sterile air, and packaged to obtain a composite microbial bacterial agent.

Embodiment 3

[0266] Embodiment 3 accounts for the percentage by weight of the total number of living bacteria of the composite bacterial powder by the number of live bacteria in the dry powder of various bacterial species:: Bacillus subtilis 10%, Aspergillus niger 10%, Bacillus colloidus 10%, Enterococcus faecalis 10%, Bacillus licheniformis 12%, Bacillus megaterium 12%, Pseudomonas fluorescens 12%, Lactobacillus plantarum 8%, Bacillus polymyxa 8%, Streptococcus thermophilus 8%. The dry bacterial powders of 10 kinds of purified bacterial strains prepared in the first step of the embodiment were mixed evenly, and the composite bacterial powder with multiple bacterial cells was injected into sterile air, and packaged to obtain a composite microbial bacterial agent.

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Abstract

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

Description

technical field [0001] The invention belongs to the field of biotechnology and environmental protection, and relates to biological fermentation treatment of breeding excrement pollution and resource utilization of organic waste, microbial degradation of antibiotics and pesticide residues for restoration of agricultural ecological environment, and in particular to a composite microorganism that degrades antibiotics and pesticide residues Bacteria and its preparation and application. Background technique [0002] With the large-scale and industrialization of the breeding industry in our country, animal diseases frequently occur in the breeding process. In order to prevent and control animal diseases, various chemical drugs, vaccines, antibiotics, etc. Some drugs are excreted through feces and urine, and high concentrations of antibacterial drug residues are formed in feces and urine. [0003] According to the existing aquaculture manure treatment technology, there are two way...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/125C12R1/10C12R1/685C12R1/07C12R1/46C12R1/25C12R1/12C12R1/11C12R1/39C12R1/01
CPCC12N1/20C05F11/08C12N1/14
Inventor 何圣平凌仕信
Owner 中山市润泽生物科技有限公司
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