Compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof
A compound microbial agent and pesticide residue technology, which is applied in the fields of biotechnology and environmental protection, can solve the problems of high incidence of pests and diseases, long cycle, and inability to solve the pollution of pesticide and antibiotic residues, etc.
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Embodiment 1
[0152] Embodiment 1 Adopt the following steps to prepare composite microbial inoculum of the present invention:
[0153] 1. Ten strains of Bacillus subtilis, Bacillus licheniformis, Aspergillus niger, Bacillus colioids, Thermococcus, Enterococcus faecalis, Lactobacillus plantarum, Bacillus polymyxa, Bacillus megaterium and Pseudomonas fluorescens were tested separately Screening of strains and optimization of culture conditions to obtain purified strains; high-density culture of purified strains, inoculation into shake flasks for activation culture, inoculation of activated cultured strains into fermenters for expansion culture; after expansion culture, the The fermented bacterial liquid of the single strain obtained by expanding the culture respectively is dehydrated and dried to obtain the dry powder of the above-mentioned 10 bacterial strains; the specific steps are as follows:
[0154] 1. Prepare the dry powder of Bacillus subtilis by the Bacillus subtilis strain according...
Embodiment 2
[0265] Embodiment 2 According to the percentage by weight of the number of live bacteria in the dry powder of various strains of bacterial cells accounting for the total number of live bacteria in the composite bacteria powder: Bacillus subtilis 15%, Aspergillus niger 15%, Bacillus colloidus 15%, Enterococcus faecalis 15%, lichen Bacillus 8%, Bacillus megaterium 8%, Pseudomonas fluorescens 8%, Lactobacillus plantarum 5%, Bacillus polymyxa 5%, Streptococcus thermophilus 6%. The dry bacterial powders of 10 kinds of purified bacterial strains prepared in the first step of the embodiment were mixed evenly, and the composite bacterial powder with multiple bacterial cells was injected into sterile air, and packaged to obtain a composite microbial bacterial agent.
Embodiment 3
[0266] Embodiment 3 accounts for the percentage by weight of the total number of living bacteria of the composite bacterial powder by the number of live bacteria in the dry powder of various bacterial species:: Bacillus subtilis 10%, Aspergillus niger 10%, Bacillus colloidus 10%, Enterococcus faecalis 10%, Bacillus licheniformis 12%, Bacillus megaterium 12%, Pseudomonas fluorescens 12%, Lactobacillus plantarum 8%, Bacillus polymyxa 8%, Streptococcus thermophilus 8%. The dry bacterial powders of 10 kinds of purified bacterial strains prepared in the first step of the embodiment were mixed evenly, and the composite bacterial powder with multiple bacterial cells was injected into sterile air, and packaged to obtain a composite microbial bacterial agent.
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