295 results about "Polymyxin" patented technology

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

Novel protected cyclopeptide intermediates are prepared from polymyxin B are used to synthesize new peptide antibiotics. Intermediates are readily derivatized and deprotected to provide new families of antibiotics, which have potent anti-bacterial activity against gram-negative bacteria; but also are useful and potent against gram-positive bacteria.

An endotoxin adsorbent used for blood perfusion is prepared from agat through adding the aqueous solution to the liquid mixture of toluene and CCl4 to obtain spherical agar, activating by epoxy chloropropane under alkaline condition, reacting on diamine and then on glutaraldehyde to obtain high-strength agar beads used as the carrier of adsorbent, reacting on the amino contained ligand (polymyxin B), and reducing by NaBH4. Its advantages are high compatibility to blood and high effect on absorbing endotoxin from blood.

The present invention provides a new class of polymyxin derivates useful for treating bacterial infections, especially Gram-negative infections, that have reduced renal cytotoxicity.

Provided herein are novel compounds and novel protected compounds that can be derived from polymyxin, including, e.g., polymyxin A. The novel compounds have antibacterial properties against a diverse range of Gram negative bacteria and reduced toxicity compared to polymyxins such as polymyxin A. Also provided are antibacterial pharmaceutical compositions containing the novel compounds and novel protected compounds, as well as methods for preparing the antibacterial compounds and protected compounds.

A pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent, wherein the enhancer of an antimicrobial agent is an inhibitor of gene, that by inactivating the gene product potentiates the effectiveness of the antimicrobial agent. In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier. In some embodiments, the antimicrobial agent is an antimicrobial peptide such as a polymyxin, for example but not limited to colistin. In some embodiments of the present invention provides methods to treat and / or prevent infection of a subject with a microorganism by administering a pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent. In some embodiments, the present invention provides methods to inhibit growth of a microorganism by administering a pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent.

The invention relates to the fields of analytical chemistry and food safety, particular to a method for detecting the residual quantity of multiple polypeptide veterinary drugs in animal-derived foods. The method comprises the following steps of: processing a sample with a TCA(trichloroacetic acid) and acetonitrile system for depositing proteins; extracting with a carbinol and 0.1% formic acid aqueous solution system; purifying with a Oasis HLB solid phase extraction column; performing gradient elution with an Eclipse XDB-C18 analytical column in the presence of an acetonitrile and 0.1% formic acid aqueous solution used as a mobile phase; and then performing electrospray and positive ion scanning mode separation for finally detecting four polypeptides. The limits of the four polypeptides, namely colistin, bacitracin A, polymyxin B and virginiamycin M, are 25 micrograms / kilogram, 100 micrograms / kilogram, 250 micrograms / kilogram and 120 micrograms / kilogram respectively; the recovery rates of colistin, bacitracin A, polymyxin B and virginiamycin M are 74.9-88.1%, 76.2-89.0%, 76.6-81.2% and 77.3-86.9% respectively; and the coefficients of variation (CV%) of colistin, bacitracin A, polymyxin B and virginiamycin M are 5.7-15.1%, 7.2-15.7%, 6.0-8.0% and 9.5-18.6% respectively. The detection limit, the recovery rate, the accuracy and other technical indexes all meet related detection requirements at home and aboard.

The invention discloses an analytical detection method of polymyxin sulfate B, comprising the steps of analytically detecting polymyxin sulfate B via high-performance liquid chromatography in combination with an evaporative light scatterer. The method allows four components in polymyxin sulfate B to be fully separated, has high specificity, is simple to perform, is free of disturbance by other organic impurities, is approximately effective to HPLC (high-performance liquid chromatography) of EP 9.0 bulk pharmaceutical chemicals in terms of high sensitivity, good simplicity and high speed, and allows the contents of components in a polymyxin sulfate B preparation to be measured more accurately.

An endotoxic adsorpting method for treating endotoxemia and anaphylactic reaction caused by endotoxemia is prepared through activating the hydroxy group of agarose gel or DVA as carrier by cyanogen bromide or epoxy chloropropane or sulfuryl chloride, and adding polymyxin solution.

The invention provides a method for separately preparing for polymyxin E, which uses foam separation method to extract and separate the polymyxin E from fermentation liquor. The fermentation liquor obtains polymyxin E raw medicine by first treatment, form separating, vacuum evaporation and spray drying.

The invention discloses a polymyxin derivative and a preparation method thereof. Since zymohydrolysis, fatty acid chain on the end N of polymyxin B and one diaminobutyric acid residue are removed, a 3, 4-oxamido-benzoyl group is introduced through a chemical decoration technology or a benzene ring group with two fatty acid chains is introduced, so the polymyxin derivative is obtained. The derivative can be used for the clinical treatment of infection of Gram-negative multidrug resistance bacteria.

The invention discloses a fluorescent PCR detection method for drug resistance gene mcr (mobile colistin resistance)-4 / 5 / 8. A protection primer combination provided by the invention consists of single-stranded DNA represented by sequence 1 to sequence 6 in the sequence table. The SYBR dye-based fluorescent quantitative PCR method for the three mcr genes is established. The detection method has thetechnical advantages of high speed, high sensitivity and strong specificity, and is suitable for monitoring the prevalence of polymyxin drug resistance gene mcr-4 / 5 / 8 in clinical and animal husbandry.

The combination of a polymyxin composition and a hop composition produces unexpected and synergistic activity against gram positive bacteria.

The invention discloses a process for extracting colistin sulfate through a precipitation method. The process comprises the following steps of: (a) adjusting pH of fermentation liquor containing polymyxin E with oxalic acid or concentrated sulfuric acid, and filtering with a filtering device; (b) adding 0 metal complexing agent and inorganic flocculant to the filtrate, and reacting at normal temperature for 0.5 to 4.0h; (c) heating, cooling, and vacuum-filtering the reactive liquid; (d) adjusting pH of the filtrate above with aqueous alkali, separating the solid from the liquid to obtain a filter cake, dissolving the filter cake with sulfuric acid solution so as to obtain acidizing fluid, adding potassium permanganate and activated carbon to the acidizing fluid respectively, agitating for0.5 to 2.0 hours, and then vacuum-filtering to obtain colistin sulfate liquid; and (e) centrifugally spray-drying the colistin sulfate liquid. With the adoption of the process, the common problems ofthe existing process for purifying fermentation liquor that the steps are complicated, the period is long, the labor intensity is relatively high, and the wastewater is difficult to treat and the like can be overcame.

The invention discloses a broad-spectrum biological bactericidal air filtration material and a preparation method thereof. The invention is not only applicable to the general families and business departments with the need of air purification, but is also applicable to the semiconductor, pharmaceutical, hospitals and other environments with higher requirements of cleanliness. The broad-spectrum biological bactericidal air filtration material of the invention comprises at least one biological anti-bacterial agent and glass fiber filter paper, and the biological anti-bacterial agent is lysozyme, protamine sulfate, poly-lysine, nisin, polymyxin sulfate, glycine or natamycin. The preparation method of the invention is that the air filtration material is prepared by silanization, hydroformylation, condensation reaction and removal of the physically absorbed anti-bacterial agent. The biological bactericidal air filtration material prepared by the invention has low consumption of the anti-bacterial agent and no influence on the resistance of the filtration material; the sterilization rate is high, most of the killing rate of bacterial, spores and viruses is more than 99 percent, and the fungal growth is inhibited.

The invention relates to a sorbent for removing endotoxins from a biological liquid, the sorbent comprising a water-insoluble, porous support and polymyxin which is immobilized on the support, said support having a neutral, hydrophobic surface and the polymyxin being immobilized on the surface of the support via hydrophobic interaction. The sorbent is especially used in extracorporeal blood purification, especially for the treatment of individuals suffering from a sepsis.

The invention provides multiple PCR primers for detecting polymyxin drug resistance genes. The multiplex PCR primers are composed of the following five primer pairs, including the primer pair shown inSEQ ID No:1 and SEQ ID No:2, the primer pair shown in SEQ ID No:3 and SEQ ID No:4, the primer pair shown in SEQ ID No:5 and SEQ ID No:6, the primer pair shown in SEQ ID No:7 and SEQ ID No:8 and the primer pair shown in SEQ ID No:9 and SEQ ID No:10. The invention further provides a detection kit and a detection method for detecting the polymyxin drug resistance genes. The primers are designed based on a gene sequence of mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5, and the drug resistance genes corresponding to the sizes of different PCR products are distinguished. After a reaction system and conditions are optimized, finally, a complete multi-PCR reaction system is determined. The quick and effective detection method is provided for simultaneously detecting the polymyxin drug resistance genes-a mcr gene family and has high sensitivity and specificity.

The present invention relates to color developing culture medium, detecting kit and detection method for Vibrio parahaemolyticus, and belongs to the field of food microbe safety monitoring technology. The detecting kit consists of color developing culture medium comprising bacterial specific enzyme in 0.05-0.95 g and mixed color developing substrate Y-glucoside, enrichment liquid A of pancreatic casitone liquid culture medium and enrichment liquid B comprising sodium chloride, polymyxin and broth. The detection method includes the enrichment culture of the sample in enrichment liquid A and enrichment liquid B, the culture of the two parts of enrichment cultured liquid in the color developing culture medium, and judging whether to exist Vibrio parahaemolyticus according to whether to have smooth raised purple colony of 2-3 mm diameter. The present invention has low cost, high detection sensitivity, short detection period and other advantages.

The invention discloses a microbial feed additive for treating piglet yellow and white dysentery and a preparation method thereof. The additive is prepared from the main components of bacillus subtilis and saccharomyces cerevisiae which are mixed with baking soda, xylo-oligosaccharides, vitamins, vitamin complex, glucose and the like according to certain proportions. The microbial feed additive has the following advantages that: the active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growing process of the bacillus subtilis have obvious inhibitionto pathogens or conditioned pathogens of endogenous infection; cell wall polysaccharides of the saccharomyces cerevisiae and a great amount of nucleotides and bioactive peptides generated in the growing process of the saccharomyces cerevisiae can improve the immunity of animals, and a compound taste substance is also generated in the growing process of the saccharomyces cerevisiae to promote animal feeding; the baking soda replenishes electrolyte; the xylo-oligosaccharides selectively proliferate beneficial bacteria such as bifidobacterium and the like; the vitamins have the effects of resisting stress and replenishing nutrition; and the glucose has the effects of supplying energy and promoting healing.

Belonging to the field of animal biomedical engineering, the invention specifically discloses a preparation method of a bacteriophage lyase capable of lysing escherichia coli and salmonella. Through primer design, a lyase gene of bacteriophage ECGD1 is successfully amplified by PCR, and is transferred into an expression system to obtain a recombinant strain. IPTG induction is carried out on the recombinant strain to obtain expression, then the strain is lysed to obtain a soluble recombinant lyase of bacteriophage ECGD1, the recombinant lyase can lyse a plurality of escherichia coli and salmonella, i.e. having a wide lysis spectrum. In addition, the synergistic effect result of the recombinant lyase and polymyxin B shows that the existence of the recombinant lyase lowers the minimum inhibitory concentration of polymyxin B, i.e. the two have a synergistic bacteriostatic effect. Identification on the lysis activity of the recombinant lyase and identification on the wide lysis spectrum thereof reveal that the recombinant lyase of the bacteriophage ECGD1 has potential application value in prevention and control of escherichia coli and salmonella infection.

The invention discloses a gene fragment which has a nucleotide sequence as shown in SEQ ID NO.1; the invention also provides a vector pPlasmid-Clearance, wherein the vector pPlasmid-Clearance is constructed by inserting the gene fragment having the nucleotide sequence as shown in SEQ ID NO.1 into a resistance gene region of a conjugal transfer vector pEF01 through a homologous recombination method; the conjugal transfer vector pEF01 has the nucleotide sequence as shown in SEQ ID NO.2. The gene fragment and the vector pPlasmid-Clearance can reverse the drug resistance of drug resistant bacteria of polymyxin drug-resistant gene mcr-1 in bacterial flora of environments and animals, can block the spread of polymyxin drug-resistant gene mcr-1, and have great application potential in the prevention and clinical treatment of bacterial diseases.

The invention provides a composite hydrogel wound dressing and a preparation method thereof. The aminated collagen and oxidized sodium alginate are used as base materials; two polypeptide antibiotics,namely polymyxin B sulfate and bacitracin are loaded; and the AC / OSA-PB composite hydrogel dressing is prepared through a Schiff base reaction. The preparation method is simple to operate; the requirements on reaction conditions are low; gel can be formed in a short time, large-scale production is facilitated, and the prepared wound dressing not only has a good bactericidal effect, but also can improve the HSF cell mobility and the HSF cell proliferation rate to the maximum extent, has excellent wound healing promoting capacity, and also has mechanical properties and mechanical properties suitable for skin wounds.

The invention provides a pidotimod-containing pharmaceutical composition and a preparation method thereof. The pidotimod-containing pharmaceutical composition consists of pidotimod and any antibacterial medicament. The antibacterial medicament may be any of beta-lactams (including as penicillins, cephems and unclassical beta-lactams), aminoglycosides, polymyxins, macrolides, lincomycins, norvancomycins, tetracyclines, chloromycetins, artificial antibacterial medicaments (including sulfonamides, quinolones and furofurans), cholesterols and antiviral medicaments. The unit dosage of the pidotimod is 0.1 to 2 grams, preferably 0.4 to 0.8 gram. The dosage of the antibacterial medicament is determined according to the dosage range of the antibacterial medicament. The composition of the pidotimod and the antibacterial medicament can exogenously and directly kill pathogenic bacteria through the antibacterial medicament and mobilize endogenous anti-infection immunity through an immunomodulator to improve anti-inflection effect, and has excellent synergy.

The present invention relates to an endotoxin adsorbent which is composed by taking spherical porous cellulose as a carrier and immobilizing effective amount of polymyxin B in the endotoxin adsorbent for blood perfusion as a ligand. The preparation method of the endotoxin adsorbent includes that the spherical porous cellulose is taken as the carrier, the spherical porous cellulose first reacts with sodium periodate and is bonded with the effective amount of polymyxin B, and the endotoxin adsorbent is obtained after reduction by sodium borohydride. The endotoxin adsorbent has the advantages of stable performance, large supported quantity of polymyxin B, good adsorption effect, etc.

The invention relates to genitourinary tract microorganism cultivation and detect method thereof, in particular to a genitourinary tract ureaplasma urealyticum (Uu) and mycoplasma hominis (Mh) culture medium and a detect method thereof. The culture medium comprises a fluid medium and a solid medium which are combined when the medium is used for culturing, separating and differentiating mycoplasma. The formulation comprises essential elements such as tryptic soy broth, yeast extract, urea, arginine, L-cysteine, phenol red, HEPES liquid, calf (fetal calf) serum, manganese sulfate, vancomycin, amphotericin B, trimethoprim, polymyxin, penicillin, and the like, and thereby the nutrition for mycoplasma growth and the antibiotic combination for inhibiting undesired microbe growth are optimized, no millipore filter is needed for sample inoculation and subcultivation, and the mycoplasma grows quickly. The fluid medium can maintain and prolong the logarithmic phase and indicate the growth of the mycoplasma, and the solid medium can used for observing Uu and Mh bacterial colonies, which has large background reflectance, thereby being easy for differentiation and significantly increasing the sensibility and the specificity of genitourinary tract mycoplasma detect.

The invention provides a method for producing polymyxin E through fermentation and foam separation coupling. According to the method, polymyxin E is prepared by a circulating reflux device formed by a peristaltic pump through fermentation, film separation and foam separation coupling. During coupling operation, fermentation liquor enters a foam separation tower through a film filter by means of the peristaltic pump, products and a liquid pass through the film while cells are intercepted in a fermentation tank, fermentation continues to be performed, and constancy of bacteria is guaranteed; a foam distributor is mounted at the bottom of the foam separation tower, bubbles are produced through air blowing, foam separation is performed, part of nutrients enter the foam separation tower through the film filter, a remaining liquid produced after foam separation returns to the fermentation tank in a circulating reflux manner, and sufficient utilization of the nutrients is guaranteed; coupling outside a tank body is adopted, operation conditions for fermentation and operation conditions for foam separation are controlled respectively, and the best operation conditions can be met.

The present invention discloses one kind of fertilizer plus farm chemical composition, which consists of ferrous sulfate 15-45 weight portions, ammonium molybdate 0.5-5 weight portions, magnesium sulfate 5-15 weight portions, composite amino acids 5-14 weight portions, zinc sulfate 1-10 weight portions, copper sulfate 0.5-5 weight portions and polymyxin 1-5 weight portions, and may include optional carrier, surfactant, dispersant and agricultural auxiliary agent. The fertilizer plus farm chemical composition has excellent preventing and controlling effect on the fungal, bacterial and physiological diseases of soybean and other plants.

The invention discloses an antibacterial drug composition for treating Gram-negative bacterial infections. The composition is prepared from TNP-2092 and a cell membrane penetrant, wherein the cell membrane penetrant is polymyxin B or polymyxin E. According to the antibacterial drug composition for treating Gram-negative bacterial infections, the Gram-negative bacterial infections are treated through the combined use of TNP-2092 and the cell membrane penetrant, the antibacterial activity of the composition is enhanced compared with the use of TNP-2092 or the cell membrane penetrant alone, and the composition has an antibacterial synergistic effect and can be used for treating the Gram-negative bacterial infections, including drug-resistance bacterial infections.

The invention discloses a method for segregating and analyzing polymyxin E methyl sodium sulfnate and isomer or analog thereof successfully by utilizing alkyl bonded silica packed column. The method provided by the invention is characterized in that the condition of the liquid chromatography is that the alkyl bonded silica packed column is a stationary phase, and a mobile phase is a mixed solvent composed of an organic solvent and aqueous acid, wherein the C18 alkyl bonded silica gel packed column of Optimapak corporation is taken as the stationary phase, and when acetonitrile and 0.05% of trifluoroacetic acid water solution gradient are eluted based on the volume ratio of (20:80)-(50:50), the good segregation effect is achieved.

The invention provides an arenicola cristata antibacterial peptide NZ17074 derived peptide N6 and application thereof. An antibacterial peptide NZ17074 molecular structure is optimally designed to obtain a novel derived peptide N6, and the amino acid sequence of the novel derived peptide N6 is shown as SEQ ID NO: 1. The antibacterial peptide N6 provided by the invention has a very good sterilization effect on Gram-negative bacteria, and is used for remarkably reducing the haemolytic activity on mouse erythrocytes and the cytotoxicity on mouse original macrophages. A mouse toxemia model test result shows that the antibacterial peptide N6 also has an endoxemia resisting effect, and can be used for remarkably improving the survival rate of mice, and the effect of the antibacterial peptide N6 is better than that of an antibiotic polymyxin. The antibacterial peptide N6 has small molecular weight and is easily artificially synthesized; the antibacterial peptide N6 is a low-molecular-weight polypeptide with extremely good application value, can be used for preparing novel antibacterial and anti-infection medicines and the like, and has a good application prospect.