The invention discloses a method for extraction of colistin sulphate. The method comprises the following steps of 1, a fermentation broth preparation process comprising adjusting a pH value to 4.0 by sulfuric acid, 2, a ceramic membrane filtration process comprising filtering the fermentation broth by a ceramic membrane, collecting a filtrate, and adjusting a pH value of the filtrate to 4.5-5, 3, a continuous moving bed absorption-desorption process, wherein through the continuous moving bed absorption-desorption process, a discharged liquid is obtained, 4, a discharged liquid decoloration process, 5, a nanofiltration concentration process, wherein in the nanofiltration concentration process, nanofiltration membrane pore sizes are in a range of 2100-400Da molecular weight cut-off and 10-20 times concentration is realized, and 6 a spray drying process. The method has a total colistin sulphate yield of 86 to 91% and has good energy-saving and emission-reduction effects.