35 results about "Bacterial pneumonia" patented technology

The invention relates to the technical field of probiotic preparations, in particular to an Ackerman bacterium probiotic preparation and application of the Ackerman bacterium probiotic preparation in prevention of bacterial pneumonia of chicks, the Ackerman bacterium probiotic preparation is prepared from the following raw materials in parts by weight: 40-60 parts of compound probiotics, 10-25 parts of prebiotics, 10-18 parts of a carrier and 8-15 parts of a freeze-drying protective additive, the preparation method of the Ackerman bacteria probiotic preparation comprises the following steps: adding the prebiotics and the freeze-drying protective agent into sterile water, and uniformly stirring to prepare a mixed solution; and fully mixing the composite probiotics with the mixed solution and the carrier, performing freeze drying, controlling the water content to be 3-5% after drying, and performing granulation to obtain the Ackerman bacteria probiotic preparation. The Ackerman bacterium probiotic preparation can significantly reduce the morbidity and mortality of bacterial pneumonia of chicks, and provides a new efficient and green disease prevention and control strategy for the poultry breeding industry.

According to the magnetic multifunctional nano-drug, on the basis of traditional ZIF-8, iron ions and defect structures are introduced, a synergistic antibacterial mode of magnetocaloric therapy-sonodynamic therapy-chemodynamic therapy is established for the first time, heat is generated under an alternating magnetic field, and the magnetic multifunctional nano-drug is used for treating bacterial pneumonia. Active oxygen is rapidly generated under ultrasound, existence of iron ions causes Fenton reaction to achieve chemical power therapy and induce ferroptosis to achieve synergistic antibiosis, release of zinc ions promotes polarization of macrophages to reduce inflammation of disease sites, the problems that focus depth is deep, drug resistance is generated, single treatment effect is poor and the like can be solved, and the treatment effect is good. A new thought is provided for treating the bacterial pneumonia.

The invention discloses an integrated nano-enzyme with multiple response mechanism synergism, and a preparation method and application thereof. A Z-type heterojunction based on boron nanosheets (B NSs) and rare earth doped iron phosphide (Nd < 0.02 > Fe < 1.98 > P) is constructed for the first time, and CTAB is used for surface modification to obtain a ternary composite nano-enzyme (BFC); according to the present invention, the material is endowed with the multi-response ability (POD / GSHOx enzyme activity synergistic activation) on the bacterial infection microenvironment (low pH and high H2O2), and the nano-enzyme has excellent acoustic-magnetic responsiveness, can break through the depth limitation, and can achieve the precise and controllable generation of the reactive oxygen species (ROS). Under the acidic microenvironment of pulmonary infection, the activity of peroxidase (POD) and glutathione oxidase (GSHOx) is activated, so that efficient and controllable synergistic killing is performed on bacteria.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and a preparation method and application thereof, active ingredients of the traditional Chinese medicine composition including a fermentation product prepared by subjecting traditional Chinese medicine raw materials to anaerobic solid-state fermentation in combination with Lactobacillus helveticus and enzyme preparation; the traditional Chinese medicine raw materials include ginseng, poria cocos, Chinese yam, white lentil, lotus seed, yiyi seed, amomum villosum, platycodon grandiflorum, licorice and dried tangerine or orange peel; and the enzyme preparation includes cellulase and / or cellobiase. The application effectively promotes dissolution of effective ingredients of Shenlingbaizhu powder, significantly enriches antioxidant active ingredients in the fermentation product, relieves inhibition of oxidative stress on immune cells, and greatly improves survival rate and phagocytic efficacy of macrophages, so that the application shows a significant antibacterial pneumonia effect and has an excellent application prospect.

Compositions and methods are provided for treating bacterial infections, including those that are challenging to treat by conventional medications, such as hospital acquired bacterial pneumonia (HABP), ventilator associated bacterial pneumonia (VABP), and bacteremia, caused by carbapenem-resistant bacteria such as Acinetobacter baumannii and Pseudomonas aeruginosa.

The application discloses a dandelion extract capable of neutralizing bacterial toxins and application thereof. The dandelion extract is mainly prepared by the following method: fresh dandelion juice is squeezed, the juice is collected, the juice is centrifuged, the supernatant is collected, the supernatant is ultra-filtered, and components capable of passing through a filter membrane with a molecular weight cut-off of 100 kD and incapable of passing through a filter membrane with a molecular weight cut-off of 3 kD are collected, namely the dandelion extract. The dandelion extract obtained by the application is natural in composition, free of toxic side effects and high in biological activity. Experimental evidence shows that the dandelion extract can protect host cells from attack by directly and specifically combining with toxins, effectively neutralizes the toxicity of bacterial toxins, and has effective prevention and treatment effects on diseases such as systemic bacterial infection, wound infection and bacterial pneumonia. Therefore, the dandelion extract can be used as a new type of anti-microbial and anti-infection functional material and applied to the fields of functional food, health products, medicines or functional cosmetics.

The application discloses a traditional Chinese medicine composition for preventing and treating pig bacterial pneumonia and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following components: Ephedra, apricot kernel, gypsum, Astragalus root, Anemarrhena asphodeloides, Cape jasmine fruit, talc, licorice, honeysuckle, forsythia, rhubarb, mirabilite, peony root and kudzurpini radix. The application studies and clinically applies traditional Chinese medicine by combining traditional preparation technology with modern preparation technology, and adopts the traditional Chinese medicine compound prepared through fermentation to prevent and treat pig bacterial pneumonia, so as to solve the above problems. Through clinical application, the traditional Chinese medicine compound has a treatment effect on pig bacterial pneumonia, and the cure rate can reach 91.83%, and the cure rate can reach 95.97% after fermentation, the drug efficacy is improved, the recurrence is difficult, and there is no drug residue. In addition, the components are common traditional Chinese medicines, the cost is low, the safety is high, and there is no side effect. The application not only reduces the economic loss of breeders, but also meets the demand of people for green food, and conforms to the development trend.

The invention relates to the technical field of nano-carriers, and discloses a multifunctional nano-carrier as well as a preparation method and application thereof. The multifunctional nano-carrier comprises ginsenoside Rb1 and a cationic polymer, and the cationic polymer is one of poly (aminopropyl) biguanide (PAPB), chitosan (CS), polyacrylamide (PAM) or polyethyleneimine (PEI). The preparation method comprises the following steps: dispersing ginsenoside in normal saline, adding a cationic polymer solution, and carrying out ultrasonic treatment on the mixed solution to obtain the multifunctional nano-carrier. According to the multifunctional nano-carrier and the preparation method and application thereof, the multifunctional nano-carrier can specifically target macrophages, in a septicemia-related acute lung injury ALI model, macrophage-mediated Rb1 is promoted to be delivered to an inflammation part, inflammation is relieved, and the anti-inflammatory effect is achieved. The clearance rate of ALI related to blood infection and bacteria in a bacterial pneumonia model can be improved, and inflammation and immune response are relieved; the compound can be used for developing medicines for treating chronic inflammation.

The invention is suitable for the technical field of medical intelligent diagnosis and machine learning, and provides a pneumonia screening and risk prediction model construction method based on a reverse neural network, and the method comprises the steps: firstly carrying out the preprocessing of a bacterial pneumonia clinical symptom data set, and dividing a multi-classification problem into a plurality of binary classification tasks; secondly, constructing a CPT algorithm based on a random forest and a convex hull theory, calculating a feature weight through information gain, and screening out high-contribution symptom features; and finally, inputting the screened features into the optimized BPNN, and obtaining a prediction model through repeated iterative training. The method has good classification accuracy, the number of the selected features is obviously reduced compared with a traditional model, and the method can be used as a clinical auxiliary diagnosis tool, is applied to clinical bacterial pneumonia infection screening and severe risk early warning, and provides decision support for timely treatment.

Methods for treating or preventing bacterial pneumonia in a subject involve administration of a Prevotella composition to the subject. The Prevotella composition may include cells, or portions thereof, of one or more of P. melaninogenica, P. buccae, P. tannerae, and P. nanceiensis. The composition may be administered by inhalation.

The present invention refers to an in vitro method for identifying biomarkers or biomarker signatures for the differential diagnosis between viral and bacterial pneumonia in a patient, for the differential diagnosis between viral and bacterial pneumonia in a patient and / or for selecting a therapy for a patient suffering from pneumonia.

The present application relates to the technical field of nanocarrier, and discloses a multifunctional nanocarrier, a preparation method and application thereof.The multifunctional nanocarrier comprises ginsenoside Rb1 and a cationic polymer, and the cationic polymer is one of polyaminopropyl biguanide PAPB, chitosan CS, polyacrylamide PAM or polyethylenimine PEI.The preparation method is as follows: the ginsenoside is dispersed in physiological saline, a cationic polymer solution is added, and the mixed solution is ultrasonically treated to obtain the multifunctional nanocarrier.The multifunctional nanocarrier, the preparation method and application thereof can specifically target macrophages, can not only promote the macrophage-mediated delivery of Rb1 to the inflammation site in a sepsis-related acute lung injury (ALI) model, relieve inflammation, but also can improve the bacterial clearance rate in blood infection-related ALI and bacterial pneumonia models, reduce inflammation and immune response, and can be used for developing drugs for treating chronic inflammation.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a composition capable of resisting bacterial pneumonia, a preparation method and application. The composition is prepared by fermenting a mixture of a traditional Chinese medicine composition, isomaltooligosacharide and mint powder through compound probiotics, and the traditional Chinese medicine composition comprises the following raw materials: 10-25 parts of loquat leaves, 5-15 parts of momordica grosvenori, 5-15 parts of liquorice, 2-10 parts of orange peel and 1-8 parts of scaphium scaphigerum. The compound probiotics are prepared from the following components in parts by mass: 1 to 2 parts of plant lactobacillus HCS03-001, 1 to 1 part of lactobacillus reuteri HCS02-001 and 1 part of lactobacillus rhamnosus HCS01-013. The medicinal and edible raw material extracting solution is used as a culture medium for fermentation, and the composition generated by combining specific lactic acid bacteria can be used for preparing bacterial pneumonia resisting products.

The invention provides application of tetrathiomolybdate in preparation of drugs for inhibiting inflammatory factor storm, and belongs to the technical field of drugs for treating inflammatory factor storm and respiratory system diseases. The general molecular formula of the tetrathiomolybdate is X < + > MoS < 42-Y < + >, and the positions of ionic bonds of four sulfur ions of the tetrathiomolybdate are interchangeable; x + and Y + are the same or different cations; the positive ions are inorganic positive ions and / or organic positive ions which can be dissociated in a solution to generate MoS < 2-> or can be subjected to enzymolysis in a biological system to generate MoS < 2->. The invention discloses unique advantages of a single drug scheme in inflammatory factor storm and respiratory system related diseases, especially bacterial acute respiratory system inflammation, for the first time, and tetrathiomolybdate is used as a single active component for preparing the drug for treating bacterial pneumonia and non-viral acute lung injury / acute respiratory distress syndrome.

The application discloses a volatile oil emulsion of phellodendri chinensis leaves and a preparation method and application thereof, and relates to the field of agricultural and sideline product processing. Specifically, the volatile oil emulsion of phellodendri chinensis leaves comprises the following components: 80-150 parts by weight of volatile oil of phellodendri chinensis leaves, 70-180 parts by volume of an emulsifier, and 1200-2000 parts by volume of water; wherein the volatile oil of phellodendri chinensis leaves is obtained by water vapor distillation. The volatile oil emulsion of phellodendri chinensis leaves has high stability, good palatability, and obvious inhibitory effect on staphylococcus aureus. In addition, the volatile oil emulsion of phellodendri chinensis leaves can effectively alleviate pneumonia of mice infected by staphylococcus aureus, inhibit the activation of NLRP3 inflammasome and the production of inflammatory factors; therefore, the volatile oil emulsion of phellodendri chinensis leaves has an auxiliary treatment effect on bacterial pneumonia and has a good application prospect.

The application belongs to the technical field of traditional Chinese medicine preparation, and discloses a traditional Chinese medicine composition, a preparation method and application thereof. One of the traditional Chinese medicine compositions comprises the following raw materials in parts by weight: Cyclobalanopsis glauca 50-80 parts, tartary buckwheat 30-50 parts, polygonatum 30-50 parts, honeysuckle 30-50 parts, Poria cocos 10-20 parts, gordon euryale seed 10-20 parts, lotus seed 10-20 parts, medlar 10-20 parts, angelica 10-20 parts, yam 5-15 parts, hawthorn 5-15 parts, and sour jujube seed 5-15 parts. The compound preparation has a good blood glucose-lowering effect, can correct the lipid metabolism disorder of diabetic rats, and reduce liver function damage, has a certain protective effect on the liver, and can be applied to the preparation of a medicine for treating non-alcoholic fatty liver. On this basis, in addition to adding part of the medicinal materials, the traditional Chinese medicine composition can also be applied to the preparation of a medicine for treating chronic obstructive pulmonary disease and a medicine for treating bacterial pneumonia.

The invention relates to the technical field of probiotic preparations, and particularly discloses a composition of Ackermann muciniphila and prebiotics and application of the composition in prevention and treatment of poultry bacterial pneumonia. The composition of Ackermann muciniphile and prebiotics is prepared by the following method: performing high-density fermentation culture on Ackermann muciniphile, and performing centrifugation, washing and freeze drying to prepare high-activity bacterial powder; the prebiotics are mixed with the high-activity bacterial powder in proportion after being purified and sterilized, and the activity of the bacterial strain in the storage and use process is protected by adopting a microcapsule embedding technology. The product can be prepared into powder, granules or liquid dosage forms, is convenient to add into poultry feed or drinking water, is added into the feed or drinking water according to the proportion of 0.1-0.5% in the susceptible period of poultry, and can remarkably reduce the incidence rate of poultry bacterial pneumonia after being continuously used for 7-14 days.

The invention discloses a composition for improving lung injury of dogs after bacterial infection as well as a preparation method and application thereof, and relates to the technical field of animal health care products. The composition is prepared from a honeysuckle flower extract, fish collagen peptide, a herba houttuyniae extract, L-carnitine, isoleucine, valine, taurine, glutamine, L-glutamic acid, L-lysine, L-threonine, L-histidine, succinic acid, L-proline, methionine, cysteine and glycine. The composition can effectively relieve dog lung epithelial cell injury caused by streptococcus pneumoniae infection, reduce intracellular lactic dehydrogenase release, improve cellular morphology and reduce inflammatory response and oxidative stress level. The composition disclosed by the invention can improve respiratory tract discomfort symptoms, such as high cough frequency, increased mucus secretion, poor mental state and the like, which still exist after the treatment of the dog bacterial pneumonia through a multi-target effect. The health recovery level of the dogs after conventional antibiotic treatment can be improved.

Provided are methods of treating hospital- acquired bacterial pneumonia and ventilator- associated bacterial pneumonia (HABP / VABP) caused by susceptible isolates of Acinetobacter baumannii-calcoaceticus complex using durlobactam and sulbactam.

The invention provides a detection reagent for lung bacterial infection detection and application thereof, and relates to the technical field of medical diagnostic preparations. The detection reagent is an atomized solution capable of being inhaled by the nose, and the atomized solution capable of being inhaled by the nose is mainly prepared from C13 urea freeze-dried powder. The detection reagent is used as a nasal inhalation atomized solution and can directly target lung pathogens in an aerosol inhalation manner, so that efficient and non-invasive bacterial pneumonia diagnosis is realized, and the problems of sampling limitation and operation risk caused by dependence of existing traditional lung inflammation diagnosis on sputum culture or invasive bronchoalveolar lavage are effectively relieved.

The invention discloses a pharmaceutical composition containing a pseudo-ginseng extract as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The invention provides a pharmaceutical composition containing a pseudo-ginseng extract, and tests prove that the pharmaceutical composition containing the pseudo-ginseng extract has a treatment effect on bacterial pneumonia induced by klebsiella pneumonia. Specifically, the pharmaceutical composition containing the pseudo-ginseng extract can significantly reduce the levels of IL-6, IL-1beta and TNF-alpha in alveolar lavage fluid; the mouse lung index is obviously reduced; the bacterial load in the lung tissue of the mouse is obviously reduced; the mouse lung tissue injury degree is obviously reduced; the number of neutrophils and the number of leukocytes in mouse blood are obviously reduced; the composition also has the effect of remarkably relieving increase of inflammatory factors in serum caused by klebsiella pneumoniae, and the effect is equivalent to or superior to that of a positive control drug ceftriaxone sodium. The invention provides a new choice and idea for clinical treatment of bacterial pneumonia.

The invention discloses a recognition instrument and method for pneumonia pathological typing. The invention relates to the technical field of biology, and discloses an identification method for pneumonia pathological typing, which comprises the following steps: S1, a blood sample is positioned on a circulation tank under the negative pressure action of an aspirating ball, and an antibody captures neutrophils; s2, after 3-7 minutes, cleaning the blood sample with normal saline under the negative pressure action of the air suction ball, and enabling the normal saline and the blood sample to be far away from the PDMS substrate circulation groove; s3, electrodes located at the two ends of the circulation groove are powered on; s4, placing the chip substrate under a microscope to observe the movement condition and track of the neutrophil, and obtaining the total migration distance, migration time and linear migration distance of the characteristic parameters of the cell; s5, the cell average migration speed and the electrotaxis index are obtained, and if the cell average migration speed and the electrotaxis index are larger than the corresponding given values at the same time, the blood sample pathological subtype is bacterial pneumonia. The kit can accurately and quickly perform pathological typing on the infantile pneumonia in the early stage of the infantile pneumonia, so as to assist clinical treatment.

The application discloses a traditional Chinese medicine composition for resisting bacterial pneumonia and application thereof. The traditional Chinese medicine composition is composed of a platycodon grandiflorum extract, a leaf of loquat extract, a polygonatum odoratum extract, a mulberry leaf extract, a lily extract, a saussurea involucrata extract, a dendrobium officinale flower extract, a nenufar extract, a momordica grosvenori extract, a noni fruit powder and an acai berry powder. In addition, the application further provides a traditional Chinese medicine liquid preparation for resisting bacterial pneumonia. Gamma-cyclodextrin is used as a taste masking agent for the first time, which is used for covering the bitter and astringent tastes caused by the platycodon grandiflorum and the leaf of loquat. Meanwhile, the gamma-cyclodextrin has the functions of solubilization and suspension, and is beneficial to improving the bioavailability of platycodin. Experiments prove that the traditional Chinese medicine composition has the remarkable effect of resisting bacterial pneumonia and is safe and free of side effects. The application provides a new technical means for treating bacterial pneumonia.

The present invention belongs to the field of biomedicine technology, and in particular to the application of acetyl-CoA in the preparation of a medicine for treating pneumonia. In the present invention, taking Klebsiella pneumoniae as a representative, the improvement effect of acetyl-CoA on bacterial pneumonia mice was explored. The results showed that acetyl-CoA can reduce the lung index of bacterial pneumonia mice to varying degrees, can reduce the bacterial load in the lung tissue of bacterial pneumonia mice, can also improve the damage of bacterial pneumonia mouse lung tissue, and can also reduce the expression level of inflammatory factors in the lung tissue of bacterial pneumonia mice and the oxidative stress level of the lung tissue. In summary, the present invention provides a new use of acetyl-CoA, which provides a new idea and theoretical basis for the treatment of bacterial pneumonia.

The invention relates to the technical field of biological medicines, and discloses application of calcium phosphate-loaded bacterial membrane vesicles in preparation of a preparation for enhancing a bacterial pneumonia prevention effect. The bacterial membrane vesicles are derived from pseudomonas aeruginosa, klebsiella pneumoniae or acinetobacter baumannii; the extraction and purification of the bacterial membrane vesicles comprises the following steps: respectively selecting standard strains, namely pseudomonas aeruginosa, klebsiella pneumoniae and acinetobacter baumannii, and activating and culturing to a logarithmic phase; after the culture is completed, collecting bacterial supernatant through room-temperature centrifugation; and purifying the BMVs crude extract by using a gel filtration chromatography column. According to the application of the bacterial membrane vesicle loaded with the calcium phosphate in preparation of the preparation for enhancing the bacterial pneumonia prevention effect, the calcium phosphate is adopted as a broad-spectrum immunologic adjuvant, an immunologic enhancement system of the calcium phosphate and the bacterial membrane vesicle is efficient, universal and high in targeting specificity, corresponding pathogenic bacterium infectious pneumonia can be accurately prevented, and the preparation method is suitable for large-scale popularization and application. The prevention efficiency of the preparation is obviously improved; meanwhile, the preparation process is simple and mild, and the biological safety is high.

The invention discloses a kit and method for detecting myxovirus drug-resistant protein A based on polypeptide and application, and belongs to the technical field of proteomics. The amino acid sequence of the polypeptide is as shown in SEQ ID No. 29. The polypeptide is obtained based on enzymolysis of an MxA standard substance and screening, a standard curve is established by utilizing the polypeptide and isotope labeled polypeptide, a biological sample is further subjected to enzymolysis to obtain a peptide fragment sample, and MxA in the biological sample can be quantified by detecting the content of the polypeptide in the peptide fragment sample. By utilizing the method disclosed by the invention, the MxA in the biological sample can be rapidly quantified, so that the viral pneumonia and the bacterial pneumonia are diagnosed and distinguished, and the accuracy rate is high.

The invention discloses an OMVs (at) Se bioactive preparation as well as a preparation method and application thereof. According to the preparation, a core-shell structure is formed by coating bacterial outer membrane vesicles with selenium nanoparticles, targeted delivery to lung macrophages is achieved, ferroptosis of the lung macrophages is effectively inhibited, inflammatory response is cooperatively regulated, the immune clearance ability is enhanced, and therefore the efficient immunotherapy effect on bacterial pneumonia is achieved.

Provided are methods of treating hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia (HABP / VABP) caused by susceptible isolates of Acinetobacter baumannii-calcoaceticus complex using durlobactam and sulbactam.

An explainable artificial intelligence system for early prediction of missed severe diagnoses in intensive care unit patients is provided. The system comprises a data processing module configured to receive and process electronic health record data including patient demographics, vital signs, laboratory results, and diagnostic codes. A machine learning module with ensemble, deep learning, and large language models analyzes processed data to predict likelihood of severe diagnoses including acute myocardial infarction, acute pulmonary embolism, bacterial pneumonia, aortic dissection, meningitis, spinal abscess, bacterial endocarditis, and arterial thromboembolism. An explainability module generates feature importance rankings and provides interpretable explanations for predictions.