The invention discloses zingiberone epoxidase
protein in
ligustrum sinense as well as a biological material and application of the zingiberone epoxidase
protein. The
amino acid sequence of the zingiberone epoxidase
protein CYP76S110 to be protected in the
ligustrum sinense is 2 in a sequence table, and the coding
nucleotide sequence of the zingiberone epoxidase protein CYP76S110 is 1 in the sequence table. According to the embodiment of the invention, after the coding
gene of the CYP76S110 protein is introduced into
yeast for expression, an obtained
recombinant yeast microsome solution containing the CYP76S110 protein can be used for catalyzing zingiberone to synthesize zingiberone
epoxide (zingiberone
epoxide), and the
recombinant yeast microsome solution can be used for catalyzing zingiberone to synthesize zingiberone
epoxide (zingiberone epoxide). The method can be applied to in-vitro
biosynthesis of the zingiberone derivative.