2213 results about "Bacteriophage" patented technology

The invention relates to a nano antibody targeting cadherin 17 and application thereof, and relates to the technical field of immunology and molecular biology, the amino acid sequence of a complementary determining region of the nano antibody targeting cadherin 17 comprises CDR1 shown as any one of SEQ ID NO: 21-32, CDR2 shown as any one of SEQ ID NO: 34-45, and CDR3 shown as any one of SEQ ID NO: 47-58. According to the invention, an alpaca immune phage display library is constructed, a series of nano antibody molecules targeting cadherin 17 (CDH17) are screened from the alpaca immune phage display library, and functions and applications of the nano antibody are verified at various levels of CDH17 antigen protein, CDH17 target cells, CDH17 CAR-T cells and the like.

The invention belongs to the field of molecular biology and immunology, and particularly relates to an anti-CD3 nano antibody capable of activating the activity of T cells as well as a preparation method and application of the anti-CD3 nano antibody. The invention provides a preparation method of the anti-CD3 nano antibody. The preparation method comprises the following steps: constructing a CD3 plasmid and an overexpression cell line, and immunizing alpaca to obtain peripheral blood mononuclear cells (PBMC) of the alpaca; extracting RNA (Ribonucleic Acid) and carrying out reverse transcription to obtain cDNA; carrying out PCR amplification on the antibody gene segment and cloning the antibody gene segment to a phage display vector; and electrically transforming the vector into SS320 competent cells to obtain the nano antibody library. After two rounds of panning, detecting and screening an antibody clone capable of being combined with the CD3 protein through ELISA (Enzyme-Linked Immunosorbent Assay); an antibody sequence is selected through sequencing, and an antibody protein is expressed by using a mammalian cell high-throughput expression system; and then detecting and screening the antibody which is only specifically combined with the CD3. The method disclosed by the invention is simple, convenient and rapid to operate, and can be used for preparing active antibodies for treating potential tumors and proliferative diseases.

The instant specification provides for evolved base editors which overcome deficiencies of those in art (including increased efficiency and / or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system. In particular, the instant specification provides for evolved cytidine base editors (e.g., based on APOBEC1, CDA, or AID cytidine deaminase domains) which overcome deficiencies of those in art (including increased efficiency and / or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system.

The invention discloses a preparation method and application of a universal renewable immunoaffinity magnetic bead, and belongs to the technical field of chemical detection.The preparation method comprises the steps that firstly, an engineered bacteriophage H6G5-M13 is constructed, then a polypeptide module is coupled to the engineered bacteriophage H6G5-M13, the polypeptide module comprises a part A, a part B and a part C from the N end to the C end, the part A is a target object recognition sequence located at the N end, and the part C is a target object recognition sequence located at the C end; the part B is a selectively added connecting sequence, the part C is an LPXTG substrate sequence and is located at the C end, and X refers to any amino acid; finally, the engineered bacteriophage is directionally coupled to the Ni < 2 + >-IDA magnetic bead through the action of Ni-6His, and then the universal and novel immunoaffinity magnetic bead is formed. The universal and replaceable immunoaffinity magnetic bead prepared by the method can be used for preparing a chemical detection reagent, and can also be used for capturing, releasing and sensing detection of cells and extracellular vesicles.

The invention provides a virulent bacteriophage and application thereof, the virulent bacteriophage is named as vBVpaPChan1 and has been preserved in China Center for Type Culture Collection on December 10, 2024, the preservation address is Wuhan University, Wuhan, Hubei, China, and the preservation number is CCTCC M 20242772. The bacteriophage is safe, environmentally friendly and free of toxic and side effects, and compared with known bacteriophages, the bacteriophage is short in incubation period, large in outbreak amount and capable of effectively killing diseases caused by vibrio parahaemolyticus chan02.

The invention discloses a novel acinetobacter baumannii phage vBAbaSqsb1 and application thereof, and belongs to the technical field of microorganisms. The bacteriophage is an Acinetobacter baumannii bacteriophage vBAbaSqsb1 which is preserved in the Guangdong Microbiological Culture Collection Center on July 5, 2024, the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 64831-B1, and the preservation address is Institute of Microbiology of Academy of Sciences of Guangdong Province. The bacteriophage can specifically recognize and split the acinetobacter baumannii so as to eliminate infection and overcome the problem of antibiotic resistance of the acinetobacter baumannii. Through discovery and application research of the phage vBAbaSqsb1, the phage vBAbaSqsb1 not only can be used for preparing a phage preparation for preventing and treating diseases caused by acinetobacter baumannii infection, but also can be used for preparing various environmental bactericides for solving potential acinetobacter baumannii in various environments. The bacteriophage vBAbaSqsb1 has the characteristics of safety, no toxic or side effect and the like, so that the problem of drug resistance caused by abuse of antibiotics can be avoided when the bacteriophage vBAbaSqsb1 is applied.

The invention discloses a lytic bacteriophage vBVcaPR25D and an application of the lytic bacteriophage vBVcaPR25D. The collection number of the lytic bacteriophage vBVcaPR25D is GDMCC (Graphical Digital Microbial Culture Collection Center) No: 65167-B1. The lytic bacteriophage vBVcaPR25D provided by the invention has a high specificity and a remarkable killing effect on vibrio cantoniensis V.campbelli LMG 11216T, and can be used for killing vibrio cantoniensis V.campbelli LMG 11216T. Besides, virulence factor genes and drug-resistant genes are not detected in the genome of the bacteriophage, so that the bacteriophage is high in safety and environment-friendly, and can be used as a bactericide for aquatic vibrio candersonii diseases.

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

The invention discloses a bacteriophage reagent production filtering device facilitating impurity cleaning, and relates to the technical field of filtering devices.The bacteriophage reagent production filtering device comprises an impurity cleaning mechanism and a collecting frame installed at the bottom of the impurity cleaning mechanism, a mixing mechanism is arranged at the top of the impurity cleaning mechanism, and a feeding pipe is arranged at the top of the mixing mechanism; the impurity cleaning mechanism comprises a filter box, a centrifugal motor is fixedly installed on one side of the middle of the filter box, the output end of the centrifugal motor is fixedly connected with a rotating block, a first rotating support is fixedly installed on one side of the rotating block, and rotating discs are symmetrically installed at the two ends of the first rotating support; a plurality of connecting sliding grooves are formed in the rotating discs, a plurality of first filter screen plates are connected between the two rotating discs in a sliding mode, full filtration of a phage reagent can be achieved by arranging the multiple sets of first filter screen plates, and impurities subjected to preliminary filtration can be filtered for the second time through the centrifugal effect of the multiple sets of first filter screen plates.

The invention discloses a lytic bacteriophage vBVcaPR24D and an application of the lytic bacteriophage vBVcaPR24D. The collection number of the lytic bacteriophage vBVcaPR24D is GDMCC (Graphical Digital Microbial Culture Collection Center) No: 65166-B1. The lytic bacteriophage vBVcaPR24D provided by the invention has a high specificity and a remarkable killing effect on vibrio cantoniensis V.campbelli LMG 11216T, and can be used for killing vibrio cantoniensis V.campbelli LMG 11216T. Besides, virulence factor genes and drug-resistant genes are not detected in the genome of the bacteriophage, so that the bacteriophage is high in safety and environment-friendly, and can be used as a bactericide for aquatic vibrio candersonii diseases.

The invention relates to the technical field of microorganisms, in particular to nocardia seriolae bacteriophage as well as a composition and application thereof. The name of the nocardia seriolae bacteriophage is RDP-NS002, the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number of the nocardia seriolae bacteriophage is CGMCC No.46674. The nocardia seriolae bacteriophage is named as RDP-NS002, and the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing nocardia seriolae; (2) preparing a product for inhibiting nocardia seriola; and (3) application in preparation of a product for preventing and / or treating fish nocardia disease caused by nocardia seriolae. The phage has the characteristics of high thermal stability, high acid-base stability and high splitting rate, has a long-acting inhibition effect, and is beneficial to prevention and treatment of nocardia seriola diseases.

The invention discloses a Klebsiella phage and application thereof, and belongs to the technical field of life science. The Klebsiella bacteriophage is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.46000, the preservation date is July 22, 2024, and the Klebsiella bacteriophage is classified and named as FK9. The bacteriophage provided by the invention is a biological means capable of efficiently killing klebsiella pneumonia, has the characteristics of high efficiency, specificity and small side effect, achieves the effect of preventing and treating pneumonia caused by klebsiella pneumonia on the basis of not destroying in-vivo flora balance, is incomparable to antibiotics and other antibacterial drugs, and has a broad application prospect. And meanwhile, the method contributes to sustainable development of a health medical system.

To provide an agent, a measurement method, and a measurement device for quickly and precisely measuring microbes.SOLUTION: An agent 1 of the present invention is an agent 1 for causing microbes being targets included in an analyte to emit fluorescent light, and the microbes being the targets are coliform bacilli, and in the analyte, the coliform bacilli are selectively caused to emit fluorescent light. In the agent 1 of the present invention is an agent 1 in which a prescribed fluorescence agent 1B and a (bacteriophage t4 and / or bacteriophage t4DNA)1A are combined. By the prescribed fluorescence agent 1B, (bacteriophage t4 and / or bacteriophage t4DNA)1A are caused to emit fluorescent light, and then the (bacteriophage t4 and / or bacteriophage t4DNA)1A which are caused to emit fluorescent light are selectively infected by the coliform bacilli being microbes which are targets included in the analyte, thereby selectively taking the coliform bacilli thereinto and causing the coliform bacilli to emit fluorescent light.SELECTED DRAWING: Figure 1

The present disclosure provides Cas9 variants, and base editors comprising these variants, that recognize non-canonical protospacer adjacent motifs (PAMs) and have less restrictive PAM requirements for editing. The present disclosure provides Cas9 protein variants comprising one or more amino acid substitutions relative to wild-type Nme2Cas9. Fusion proteins comprising the Cas protein variants described herein are also provided by the present disclosure. Further provided herein are methods for editing a target nucleic acid using the Cas variants and fusion proteins provided herein. The present disclosure also provides guide RNAs, complexes, polynucleotides, cells, kits, and pharmaceutical compositions. Further described herein are phage-assisted continuous evolution (PACE) systems, vectors, methods, and devices.

The invention discloses a method for improving generation of antagonistic substances of beneficial bacteria by utilizing bacteriophages. Under the continuous stress of the bacteriophage YL-Ste-01P, the yield of the bacteriostatic metabolic substances of the beneficial bacterium YL-Ste-01 is obviously increased. A bacterial wilt biocontrol bacterium YL-Ste-01 and a bacteriophage YL-Ste-01P are inoculated into an NA liquid culture medium for co-culture to obtain a culture solution, the culture solution is centrifuged to obtain supernate, and the supernate is filtered by a filter membrane to obtain fermentation liquor. According to the present invention, the co-culture fermentation broth of YL-Ste-01 and YL-Ste-01P, alpha-ketoglutaric acid, guanidinopropionic acid and fumagillin have significant inhibition effects on ralstonia solanacearum; the invention discloses application of an antagonistic substance in preparation of a reagent for inhibiting ralstonia solanacearum or prevention and control of bacterial wilt. The invention discloses application of alpha-ketoglutaric acid, guanidinopropionic acid or fumagillin to preparation of a reagent for inhibiting ralstonia solanacearum or prevention and control of bacterial wilt.

The invention discloses stenotrophomonas maltophilia bacteriophage, a composition and application thereof. Relates to the technical field of biology, the stenotrophomonas maltophilia bacteriophage comprises a bacteriophage vBSma-SZP9, the taxonomic name of the bacteriophage vBSma-SZP9 is Snotrophomonas phage, the Latin literature name of the bacteriophage vBSma-SZP9 is Snotrophomonas phage, the bacteriophage vBSma-SZP9 is preserved in the Guangdong Microbiological Culture Collection Center, the preservation address is the fifth floor of the building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, the preservation number is GDMCC No: 64509-B1, and the preservation date is May 16, 2024. The bacteriophage disclosed by the invention is wide in host spectrum, strong in infection ability and wide in temperature and acid-base tolerance range.

Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and / or positive selection of desired properties.

A method for predicting the relationship between a bacteriophage and a bacterial infection, comprising a learning model for a bacteriophage-host infection relationship and a construction method therefor. The method for constructing a learning model for a bacteriophage-host infection relationship comprises: training a learning model by using data of a training set of bacteriophages and bacterial strains, the data of the training set of bacteriophages and bacterial strains comprising genome data and phenotypic assay data. A method for using the learning model for a bacteriophage-host infection relationship to predict the bacteriophage that will infect bacteria and a method for predicting the bacteria infected by a bacteriophage, and a computer-executable medium comprising a computer program for performing the methods.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention belongs to the technical field of biology, and relates to a recombinant mycobacteriophage capable of enabling host bacteria to express nano luciferase as well as a preparation method and application of the recombinant mycobacteriophage. The recombinant mycobacterium fluorescent bacteriophage capable of enabling the host bacteria to express the nano luciferase comprises an expression element for expressing the nano luciferase in mycobacteria, the expression element contains a gene nluc required by luminescence and a mycobacterium strong promoter, and the upstream of the gene nluc is connected with a promoter sequence to ensure the normal expression of downstream genes. The base sequence is as shown in SEQ ID NO: 1. The preparation method comprises the following steps: carrying out enzyme digestion on a bacteriophage skeleton to form a linear sequence, enabling the linear sequence to be recombined with a target fragment containing a gene nulc, connecting the enzyme-digested bacteriophage skeleton with the target fragment containing the gene nluc, carrying out in-vitro packaging, transferring into an escherichia coli competent cell, and carrying out in-vivo amplification in escherichia coli; the method comprises the following steps: extracting positive phagocytids of recombinant escherichia coli, electrically transferring the phagocytids into Mycobacterium smegmatis, and culturing the phagocytids on a double-layer agar plate to obtain the bacteriophage. According to the application of the recombinant mycobacterium bacteriophage in the mycobacterium rapid detection and / or drug sensitivity detection reagent, the method is simple, convenient and rapid, the sensitivity is high, and the repeatability is strong.

The present invention relates to a method and a composition for identifying phages. The method comprises the steps of: - providing a phage composition comprising at least two different phages, each of whose genetic code is known at least in part, - incubating a bacterial sample with the phage composition, - adjusting and maintaining the bacterial sample incubated with the phage composition under conditions in which a phage can use a bacterium as a host cell, - performing a quantitative polymerase chain reaction using primer pairs, each specific for a phage, and - identifying the phage(s) whose reproduction is favored by the presence of a host cell suitable for these phages in the bacterial sample. The invention further relates to a method for quantifying phages in a sample.

The invention relates to a polypeptide NbG7-4 capable of identifying and neutralizing SFTSV, which comprises three complementary determining regions CDR1-3, and the sequences of the three complementary determining regions CDR1-3 are respectively shown as SEQ ID NO: 1-3. Nanometer antibody drug development and diagnostic kit research and development are carried out on SFTSV which is high in fatality rate but lacks effective vaccines and specific antiviral drugs, the nanometer antibody VHH which is specifically combined with GN is screened through the platform technology of preparing GN protein, inactivating viruses, immunizing alpaca, displaying nanometer monoclonal antibodies through a phage library and the like, the CDR sequence of the nanometer antibody VHH is identified, and the SFTSV with the high fatality rate is obtained. A humanized antibody NbG7-4 is constructed; meanwhile, the curative effect of the NbG7-4 in treating the SFTSV infection is evaluated at the in-vitro cell level. The invention provides a potential nano antibody new drug for clinical treatment of SFTSV, and also provides a corresponding detection kit for diagnosis of SFTSV.

According to the application of the bacteriophage to reduction of cholera transmission, the bacteriophage phi YSZKA is preserved in the China Center for Type Culture Collection on August 1, 2018, the preservation number is CCTCC NO: M 2018513, and the bacteriophage phi YSZKA is classified and named as klebsiella pneumoniae bacteriophage phi YSZKA. The method solves the problems of secondary pollution, wide transmission of vibrio cholerae and the like of the traditional split bacteriophage. The application of the bacteriophage can reduce the toxin production efficiency of vibrio cholerae in oceans, soil and sewers, thereby providing more possibilities for prevention and control of cholera epidemic situation and alleviation of cholera symptoms.

The invention discloses a bacteriophage host prediction method based on a weighted integrated convolutional neural network. The method comprises the following steps: obtaining a joint feature vector for an input bacteriophage protein sequence and a host receptor protein sequence; taking the bacteriophage-host pair as a positive sample, randomly selecting non-host bacteria for each bacteriophage to generate a negative sample, dividing the negative sample into M subsets, and combining each subset with all the positive samples to form M balance training subsets; for each balance training subset, training an independent one-dimensional convolutional neural network model; independently training a one-dimensional convolutional neural network sub-model for each balance training subset to obtain a plurality of sub-models with different performances; and distributing weights based on the performance indexes of the sub-models on the verification set, performing weighted fusion on the prediction probabilities of the M sub-models, and outputting a final host interaction probability. The method realizes accurate prediction of phage-host interaction on the premise of only needing basic sequence information, and has the advantages of light weight, high efficiency and wide applicability.

The invention discloses a lytic bacteriophage vBVpaSR40Z and application of the lytic bacteriophage vBVpaSR40Z to prevention and control of vibrio parahaemolyticus in an aquaculture environment. The preservation number of the vibrio parahaemolyticus phage vBVpaSR40Z is as follows: GDMCC No: 67440-B1, and the vibrio parahaemolyticus phage vBVpaSR40Z is The R40Z can be used for cracking multiple strains of vibrio parahaemolyticus and vibrio alginolyticus, has the capability of adapting to an aquaculture environment, and keeps relatively high activity within the range of 4-25 DEG C and pH of 4-10; no virulence gene or drug-resistant gene is detected through genome analysis, and phylogenetic and whole genome comparison shows that the similarity between R40Z and a related phage is lower than a species level threshold value, so that R40Z is a new phage species; the R40Z can obviously inhibit the growth of a host under various infection complex numbers. The R40Z has the advantages of strong splitting capacity, good environmental stability, high genome safety, unique evolutionary characteristics and the like, and is an aquatic vibriosis biological prevention and control bacteriophage with application potential.

The invention relates to the technical field of polypeptide cyclization methods, in particular to a preparation method for constructing a phage display cyclopeptide library based on phthalic dicarboxaldehyde, which comprises the following steps: carrying out click chemical reaction on phthalic dicarboxaldehyde (OPA) and lysine and cysteine in an unprotected peptide sequence under a neutral or alkaline condition to form cyclopeptide; constructing a phage cyclic peptide library on the basis of the reaction property that the OPA molecule is efficiently biocompatible with sulfydryl and amino groups; a polypeptide library containing cysteine and lysine is displayed on a bacteriophage, the polypeptide library is cyclized by utilizing OPA (o-phthalaldehyde), and a cyclic peptide ligand with specific binding is screened out aiming at a target protein. In the strategy of performing polypeptide cyclization by using o-phthalaldehyde, it is found that o-phthalaldehyde has good reaction activity with sequence peptide containing cysteine and lysine, reaction conditions are simple and mild, and the o-phthalaldehyde has good biocompatibility. The reaction system does not need organic solvents or metal catalysts and is suitable for biological systems.

The invention relates to a preparation method of an efficient antibacterial material based on bionic design. The method comprises the following steps: step A, synthesizing a piezoelectric antibacterial material; and step B, combining the piezoelectric antibacterial material and a targeting material through a liposome carrier to prepare a bionic bacteriophage. According to the scheme, an innovative bionic bacteriophage concept is provided, a simple and intelligent antibacterial system is developed, reactive oxygen species (ROS) in a bacterial microenvironment is stimulated to be improved through energy conversion, bacterial cell membranes, protein and DNA are destroyed, and therefore antibacterial treatment is carried out.

The invention relates to the technical field of feed ingredient optimization, in particular to an ingredient optimization and adjustment system for a phage type antibiotic-free feed additive. Comprising the following steps: acquiring multi-dimensional environmental data and antibody content indexes and stress state information of individuals; determining a health index according to the antibody content index and the stress state information; performing batching delay analysis according to the change of the health index, and determining a lag moment; determining an effect coefficient in combination with the lagging time and the health index, determining the influence degree of the environmental data of each dimension on the batching effect, and screening to obtain an influence environmental dimension in combination with the influence degree of the environmental data of all dimensions on the batching effect and the predicted value of the environmental data in the next batch; and adjusting the ratio of additives in the same feeding stage in the next batch based on the influence environment dimension. According to the method, the ingredient adjustment logic can be optimized, and the adaptability of ingredients in a multi-dimensional complex scene is improved.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.