5results about How to "Reduce Toxicity Risk" patented technology

This invention discloses a highly stable amphotericin B liposome and its preparation method, belonging to the field of pharmaceutical formulation technology. This invention utilizes a methanol-water-chloroform ternary mixed solvent system to achieve complete molecular-level dissolution of the raw materials, eliminating the conventional drying process and directly hydrating and homogenizing. It also provides optimal formulation ratios and process parameters. The preparation method includes steps such as oil phase preparation, aqueous phase preparation, homogenization, incubation, cooling, filtration, volumetric filling, and lyophilization. This invention creatively proposes optimal formulation composition and process conditions, resulting in a product with high encapsulation efficiency, uniform particle size, and strong stability. Furthermore, the preparation process is simple and easily industrialized, solving the problems of complex preparation processes and poor stability of existing injectable amphotericin B liposomes, and possesses good clinical application value.

The application discloses a kind of preparation methods and products of biological friendly spheroid-like trimanganese tetraoxide nanometer, it is related to functional nanometer material technical field;The application includes that manganese salt is mixed with ultrapure water and obtains manganese salt solution;Catalyst, modifier are added and stirred uniformly, catalyst is selected from monoethanolamine, diethanolamine, triethanolamine, modifier is selected from polyethylene glycol, sodium alginate and its derivative, chitosan and its derivative, water-soluble cellulose and its derivative;Basic solution and acidic solution are added dropwise to adjust the pH value of manganese salt solution, ultrapure water is added and stirred uniformly and poured into hydrothermal reaction kettle;Hydrothermal reaction kettle is placed in drying oven and heated to complete reaction to obtain trimanganese tetraoxide slurry;Filter, rinse and dry to obtain trimanganese tetraoxide powder product;The application effectively reduces the biological toxicity of trimanganese tetraoxide nanoparticle, meets the strict biomedical application requirement, preparation method is simple, process condition is mild, does not need expensive equipment, and cost is conducive to control.

This invention relates to the field of daily chemical technology, and in particular to a method for preparing kava extract based on enzymatic hydrolysis technology, its products, and applications. The preparation method comprises the following steps: S01 low-temperature treatment of kava rhizomes, followed by pulverization and sieving; S02 enzymatic hydrolysis of the kava rhizome powder; S03 fermentation treatment of the kava rhizome enzymatic hydrolysis extract obtained in S02; and S04 stabilization treatment of the fermented kava rhizome extract obtained in S03. This invention employs a combination of multi-enzyme synergistic pretreatment and probiotic co-fermentation, conducted entirely in an aqueous environment, eliminating the need for organic solvents such as ethanol and methanol. The operation is safe and environmentally friendly, aligning with the development trend of green raw materials in cosmetics.

The invention provides a manioc waste liquid feed preparation process based on microbial fermentation, and belongs to the technical field of feed. According to the application, the nano calcium phosphate loaded high-valence ferric salt and the metal ion doped hydroxyapatite are introduced to solve the problems of excessive metal ions and anti-nutrient substance residues; the nano calcium phosphate loaded high-valence ferric salt efficiently oxidizes and removes cyanide ions and tannin in the fermentation liquor through ferrate loaded on the surface; oxidation products and part of ferric ions are fixed, so that inhibition on microorganisms is avoided; the metal ion-doped hydroxyapatite effectively captures excessive metal ions through ion exchange and complexation, reduces the free concentration of the metal ions, prevents adverse effects on microbial metabolism, can compete with anions in a fermentation liquid, inhibits the generation of toxic complexes, and reduces the toxicity to a fermentation system; the synergistic effect of removal of anti-nutrient substances and dynamic regulation and control of metal ions is achieved, and the nutritional value and safety of the final feed are improved.

The invention belongs to the technical field of biology, and particularly relates to a recombinant botulinum toxin A chimeric protein and application thereof. The recombinant A-type botulinum toxin chimeric protein is constructed by replacing only a C-terminal receptor binding domain or a whole receptor binding domain in a receptor structural domain on the basis of wild-type BoNT / A. The recombinant botulinum toxin A chimeric protein has substrate cleavage activity equivalent to that of wild toxin in vitro, can be effectively taken in Neuro-2a nerve cells and realize substrate cleavage, shows a significantly reduced toxicity level in animal bodies, and has a good application prospect. Wherein the AAAF chimeric protein has the characteristics of rapid effect taking and short action time, and is suitable for situations requiring short-term intervention of neural activity, such as short-term muscular tension regulation or local injection treatment; the AAFF chimeric protein has relatively long neuromuscular blocking duration and relatively high safety, and is suitable for long-term treatment of chronic nervous system diseases.