72 results about "Gynostemma" patented technology

The invention discloses a feed additive for improving animal immunity and a preparation method, and belongs to the technical field of animal feed. The nutrient components comprise the following raw materials in parts by weight: 10-20 parts of amino acids, 0.5-3.0 parts of vitamins and 15-22 parts of minerals; the traditional Chinese medicine components comprise 20-30 parts of radix astragali, 15-25 parts of radix codonopsis, 5-20 parts of rhizoma atractylodis macrocephalae, 10-20 parts of poria cocos, 10-15 parts of radix isatidis, 5-15 parts of honeysuckle flowers, 6-12 parts of echinacea purpurea, 3-12 parts of radix acanthopanacis senticosi and 3-8 parts of fiveleaf gynostemma herb; functional additives: 3-5 parts of probiotics, 2-4 parts of an enzyme preparation and 1-3 parts of an antioxidant. According to the invention, a multi-dimensional synergistic effect is achieved, and a traditional Chinese medicine immune enhancement-antiviral core formula system is formed; through cooperation with nutrients and functional additives, a composite protection system of immunity enhancement, nutrition optimization, intestinal health and stress resistance is constructed. The immunity and the disease resistance of animals are obviously improved.

The invention discloses a gynostemma pentaphyllum transcription factor GpMYC65 and application thereof. The nucleotide sequence of the gynostemma pentaphyllum transcription factor GpMYC65 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the gynostemma pentaphyllum transcription factor GpMYC65. The amino acid sequence of the protein encoded by the gynostemma pentaphylla transcription factor GpMYC65 is as shown in SEQ ID NO. 2. The heavy recombinant vector contains a gynostemma pentaphylla transcription factor GpMYC65. The invention relates to application of a Gynostemma pentaphyllum transcription factor GpMYC65 in regulating and controlling the content of dammarane type triterpenoid saponin in Gynostemma pentaphyllum. The application comprises the following steps: step 1, constructing an overexpression vector containing a transcription factor GpMYC65 encoding gene; 2, transforming the overexpression vector into gynostemma pentaphyllum leaves by using an agrobacterium-mediated method to obtain genetic transformation hairy roots, and screening and identifying positive hairy roots of an overexpression transcription factor GpMYC65; and 3, carrying out liquid culture on the screened positive hairy roots to obtain transgenic hairy roots of which the dammarane type triterpenoid saponin content is higher than that of the wild hairy roots. The content of dammarane type triterpenoid saponin can be remarkably increased by overexpressing the transcription factor GpMYC65 in the fiveleaf gynostemma herb hairy roots.

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The invention discloses a fiveleaf gynostemma herb extract and application of the fiveleaf gynostemma herb extract in preparation of an anti-fatigue product. The gynostemma pentaphyllum extract provided by the invention comprises one or more of the following compounds: (1) gynostemma pentaphyllum saponin LXXXV II; (2) (3beta, 12beta, 20S)-trihydroxy dammarane-24-ene-3-O-[beta-D-glucopyranosyl (1-> 2)-beta-D-glucopyranosyl]-20-O-beta-D-glucopyranosyl (1-> 3)-alpha-L-rhamnopyranosyl (1-> 6)-beta-D-glucopyranosyl; (3) gypenoside V; (4) (3beta, 12beta, 20S)-trihydroxy dammarane-24-ene-3-O-[6-O-acetyl-beta-D-glucopyranosyl (1-> 2)-beta-D-glucopyranosyl]-20-O-beta-D-xylopyranosyl (1-> 3)-alpha-L-rhamnopyranosyl (1-> 6)-beta-D-glucopyranoside, and (5) (3beta, 12beta, 20S)-trihydroxy dammarane-24-ene-3-O-[6-O-acetyl-beta-D- (5) gynostemma pentaphyllum saponin LXXXVIII; (6) preparing gypenoside X; (7) (3beta, 12beta, 20R)-trihydroxy-25-hydrogen peroxide group dammarane-25-ene-3-O-beta-D-glucopyranosyl (1-> 2)-beta-D-glucopyranoside, and (7) (3beta, 12beta, 20R)-trihydroxy-25-hydrogen peroxide group dammarane-25-ene-3- (8) (3beta, 12beta, 20S)-trihydroxy-24S-hydrogen peroxide group dammarane-25-ene-3-O-beta-D-glucopyranosyl (1-> 2)-beta-D-glucopyranoside, and (3) (3beta, 12beta, 20S)-trihydroxy-24S-hydrogen peroxide group dammarane-25-ene-3- And (9) the gynostemma pentaphyllum saponin LXXXVI is prepared. The fiveleaf gynostemma herb extract provided by the invention has a remarkable anti-fatigue effect.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition with hangover-alleviating and liver-protecting effects and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw material medicines: semen hoveniae, flos puerariae, rhizoma gastrodiae, pericarpium citri reticulatae, poria cocos, rhizoma alismatis, herba artemisiae scopariae, gynostemma pentaphylla, roasted rhizoma atractylodis macrocephalae, cortex magnoliae officinalis, radix paeoniae alba and honey-fried licorice root. The traditional Chinese medicine composition disclosed by the invention has the effects of relieving alcoholism, resolving damp turbidity, clearing liver and gallbladder and strengthening spleen soil. The traditional Chinese medicine composition disclosed by the invention is particularly suitable for patients suffering from alcohol fever, alcoholic liver, hyperlipidemia and liver and gall diseases mainly caused by damp turbidity and alcohol toxicity. Experimental results show that the traditional Chinese medicine composition has a good treatment effect on acute alcoholic liver injury and chronic alcoholic fatty liver, so that the traditional Chinese medicine composition has a relatively good development and utilization prospect.

This invention proposes a Gynostemma pentaphyllum tea bag and its processing technology, belonging to the field of tea bag technology. The tea bag comprises the following raw materials in parts by weight: 60-80 parts Gynostemma pentaphyllum, 6-12 parts wild chrysanthemum, 5-10 parts Acer truncatum tea, 3-6 parts nervonic acid microcapsules, and 4-8 parts licorice. The processing technology includes: raw material pretreatment, chopping, segmented roasting, pulverizing, mixing, sterilization, and packaging. This invention, through a unique formula design, effectively improves the bitter taste of Gynostemma pentaphyllum using licorice and wild chrysanthemum; it increases the water solubility of nervonic acid by utilizing the wall structure of the microcapsules, solving the problem of its insolubility in water, allowing nervonic acid to be effectively dissolved in the tea infusion, achieving the effects of brain nerve protection and repair. Simultaneously, the microcapsule protection system ensures its dispersibility and stability in the processing system; through the combination of segmented roasting and ultra-fine pulverization, it not only alleviates the cold nature of Gynostemma pentaphyllum but also significantly improves the extraction rate of effective components.

This invention belongs to the technical field of daily-use shampoo and hair care raw materials, specifically relating to a composition with hair-darkening effects and its application. The composition with hair-darkening effects provided by this invention is formulated from a compound of Platycladus orientalis leaf extract, Polygonum multiflorum extract, Eclipta prostrata leaf extract, Gynostemma pentaphyllum extract, palmitoyl tetrapeptide-10, L-arginine, and decapeptide-4. By adjusting the types and amounts of each component, the interaction between the components is improved, thereby increasing the relative activity of tyrosinase and the relative content of melanin, indicating that the components have a synergistic effect and excellent hair-darkening efficacy. The hair-darkening composition obtained by this invention is applied to the preparation of shampoo, which exhibits excellent hair-darkening effects and high safety, and has broad market application value in the field of hair-darkening or hair-protecting products.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.

The invention provides a scented tea composition with an antihypertensive effect and application of the scented tea composition, and belongs to the technical field of health-care foods. The composition comprises the following raw materials in parts by weight: 1-8 parts of white tea, 1-9 parts of rose, 1-9 parts of corn stigma, 1-5 parts of gynostemma pentaphylla, 1-5 parts of apocynum venetum tea, 1-5 parts of chrysanthemum, 1-5 parts of dandelion and 1-5 parts of cyclocarya paliurus. The raw materials synergistically exert the antihypertensive effect through the compatibility of monarch, minister, assistant and guide. Chrysanthemum is used as a monarch drug for calming liver and suppressing yang, white tea, corn stigma and the like are used as ministerial drugs for expanding blood vessels and regulating metabolism, and dandelion and rose are used as adjuvant drugs for inducing diuresis, discharging sodium and relieving nerves. The invention also provides corresponding preparation methods of the scented tea powder and tea soup. The scented tea powder and tea soup are prepared by crushing and sieving the scented tea composition or brewing with hot water. Clinical tests show that the scented tea composition can remarkably reduce systolic pressure and diastolic pressure of hypertensive patients, is suitable for preparing blood pressure reducing health-care food, is safe and does not have side effects.

The invention discloses a preparation process of selenium-enriched gynostemma pentaphylla Fuzhuan tea. The preparation process comprises the following steps: selecting materials, spreading and airing, deactivating enzymes, rolling, adding selenium, carrying out pile fermentation on mucor, drying, ageing, steaming tea for the second time, pressing, shaping and growing fungus, and ageing for the second time. By adopting the mucor pile fermentation, on one hand, the added inorganic selenium is converted into organic selenium, and the organic selenium is tightly combined with the tea leaves, so that the loss of the selenium element is avoided; and on the other hand, the process of combining mucor pile fermentation with high-temperature instantaneous fixation reduces the degradation effect of gypenoside glycosidase on gypenoside, retains most of gypenoside in the gynostemma pentaphyllum, and improves the health-care effect of the gynostemma pentaphyllum Fuzhuan tea.

Provided are a Gynostemma pentaphyllum-derived triterpenoid saponin compound and a use thereof in the preparation of anti-fatigue products. The provided triterpenoid saponin compound is selected from one of compounds 1-29. Also provided are a use of a triterpenoid saponin compound as represented by general formula I in the preparation of anti-fatigue products. Experiments show that the Gynostemma pentaphyllum-derived triterpenoid saponin compound has significant anti-fatigue activity and can be used for preparing anti-fatigue products.

The invention belongs to the field of polymer chemistry, and particularly relates to a resin for decoloring gynostemma pentaphylla and a preparation method and application thereof, and the preparation method comprises the following steps: S1, mixing a water phase and an oil phase, standing, shaping, carrying out polymerization reaction, and removing a pore-foaming agent to obtain an acrylonitrile copolymer matrix resin; s2, mixing the acrylonitrile copolymer matrix resin with a first solvent, alkali and a nucleophilic reagent, and performing aminolysis reaction to obtain amide resin; and S3, mixing the amide resin with a second solvent, and carrying out Hofmann degradation to obtain the resin for decoloring the gynostemma pentaphylla. According to the preparation method, amide resin is prepared firstly and then converted into primary amine through Hofmann degradation, weak base functional groups can be connected to decolorizing resin, weak base resin with amino (-NH2) functional groups is prepared and used for decolorizing gynostemma pentaphylla, and the decolorizing rate is increased and the loss of active ingredients is reduced.

The invention discloses a gynostemma pentaphyllum GpMYC2-1 gene, which has a base sequence as shown in SEQ ID NO.1, the gene is obtained by cloning from gynostemma pentaphyllum leaves for the first time, and a foundation is laid for research on synthesis and accumulation of gynostemma pentaphyllum secondary metabolites by the GpMYC2-1 gene; the invention also discloses a construction method of a recombinant vector plasmid of the GpMYC2-1 gene of the Gynostemma pentaphylla. The construction method comprises the following steps: constructing a vector for over-expressing GpMYC2-1; the invention also discloses an expression method of the gynostemma pentaphyllum GpMYC2-1 gene, a hairy root system of the gynostemma pentaphyllum GpMYC2-1 gene is obtained and applied under a cadmium stress condition, and the GpMYC2-1 gene is indicated to be not only a regulatory gene for biosynthesis of gynostemma pentaphyllum saponin, but also a key transcription factor for biosynthesis of cadmium stress response gynostemma pentaphyllum saponin.

The invention discloses a method for promoting conversion of rare ginsenoside in gynostemma pentaphylla and a therapeutic effect of a prepared product on metabolic syndrome. Specifically, the invention discloses a method for promoting conversion of rare ginsenoside in gynostemma pentaphylla, and the method comprises the following steps: (1) taking a proper amount of gynostemma pentaphylla medicinal material dried in the shade, crushing, and adding water for infiltration; (2) soaking for a period of time at a certain water bath temperature; (3) evaporating to dryness, adding ethanol or methanol with a certain concentration, carrying out heating reflux extraction or ultrasonic extraction, cooling an extracting solution, filtering, and carrying out vacuum concentration to obtain a concentrate; and (4) loading the concentrate on macroporous resin, adsorbing, eluting, and collecting the 60-80% ethanol elution part to obtain the total saponins dried in the shade. The method is mild in reaction condition, high in efficiency, high in specificity and free of pollution, and the prepared product is high in rare ginsenoside content and can be used for preventing and / or treating metabolic diseases such as non-alcoholic fatty liver diseases.

This invention belongs to the field of microbial technology and discloses a strain of *Schizophyllum commune* that promotes seed germination in plants of the *Schizophyllum* genus. Schizophyllum commune The present invention provides strain pb5, with accession number CGMCC No. 42595. It also provides a microbial preparation containing strain pb5 and a method for promoting seed germination of *Gynostemma* species. The strain pb5 of this invention can promote the germination of *Gynostemma* species, especially *Gynostemma* seeds, and has significant application value for the sexual reproduction and resource conservation of *Gynostemma* species.

The application discloses a method for promoting transformation of rare ginsenoside in gynostemma pentaphyllum, and comprises the following steps: (1) taking a proper amount of dried gynostemma pentaphyllum medicinal material, crushing and then adding water for soaking; (2) soaking for a period of time under a certain water bath temperature; (3) after steaming and drying water, adding a certain concentration of ethanol or methanol, heating and refluxing extraction or ultrasonic extraction, filtering after cooling the extract, reducing pressure concentration, and obtaining a concentrate; and (4) loading the concentrate on a macroporous resin, adsorbing, eluting, collecting 60-80% ethanol elution part, and obtaining dried total saponin. The method has the advantages of mild reaction condition, high efficiency, strong specificity, no pollution, high content of prepared product rare ginsenoside, and can be used for preventing and / or treating metabolic diseases such as non-alcoholic fatty liver disease.

The invention relates to the technical field of biotechnology and pharmaceutical engineering, in particular to a fiveleaf gynostemma herb component separation method and system based on ultrasonic-assisted membrane combining.The method comprises the steps that an initial fiveleaf gynostemma herb set is obtained, a target fiveleaf gynostemma herb set is obtained based on the initial fiveleaf gynostemma herb set, and a fiveleaf gynostemma herb experimental group set is extracted from the target fiveleaf gynostemma herb experimental group set; obtaining a target crushing curve based on the gynostemma pentaphyllum experimental group and the crushing parameter set, extracting a target crushing parameter group from the target crushing curve, obtaining analyzed crushed gynostemma pentaphyllum by using the target crushing parameter group, obtaining an analyzed gynostemma pentaphyllum mixed solution based on the analyzed crushed gynostemma pentaphyllum, and determining a target ultrasonic parameter group based on the ultrasonic parameter set and analyzing the gynostemma pentaphylla mixed solution, obtaining a target gynostemma pentaphylla crude solution by using the target ultrasonic parameter group, obtaining a target screening parameter group based on the initial screening parameter set, and obtaining a target extracting solution by using the target screening parameter group. According to the method, the gynostemma pentaphylla component separation accuracy and efficiency can be improved.

The invention relates to a gynostemma pentaphylla oxidized squalene cyclase gene GpOSC2 and application thereof, and belongs to the technical field of genes. The amino acid sequence of the gynostemma pentaphylla oxidized squalene cyclase GpOSC2 is as shown in SEQ ID NO. 1. A coding sequence for coding the gynostemma pentaphylla oxidized squalene cyclase GpOSC2 gene is a nucleotide sequence as shown in SEQ ID NO. 2. According to the present invention, the gene is adopted to carry out fermentation so as to obtain Dammarenediol-II, lupeol, lanosterol, cycloartenol and alpha-amyrin alcohol, such that the application prospect is good, and the promotion and the application are easy.

1. Name of the design product: Tea packaging set (Gynostemma pentaphylla series 1). 2. Use of the design product: The design product is used for packaging tea. 3. Design points of the design product: Combination of shape and pattern. 4. Picture or photo best indicating the design points: Refer to the drawings.

A rapid detection and visualization method for triple RPA-LFA targeting necrophilic flies includes the following steps: Step 1: DNA amplification reaction; DNA is extracted from *Gynostemma pentaphyllum*, *Gynostemma spp.*, *Variegata spp.*, *Variegata spp.*, *Bulbus rubrothes*, *Gynostemma pentaphyllum*, *Flyflies fasciatus*, and *Heterodon spp.* using the SteadyPure Universal Genomic DNA Extraction Kit; the concentration and purity of the genomic DNA of the above species are assessed using a Nano Drop 2000 spectrophotometer; the reaction is performed according to the instructions of the DNA isothermal rapid amplification kit; the reaction system uses Buffer A, a primer mixture consisting of 3 pairs of primers; the mixture is incubated in a metal bath to perform the RPA reaction and obtain the amplified product; Step 2: Visual detection; Mixed amplification product: the amplified product is mixed with dd water to obtain a mixed amplification product; the mixed amplification product is dropped onto the LFD test strip sample pad, and then the result is displayed. This invention features no dependence on a sequencer, simple operation, rapid detection, and visualization.

The invention discloses a formula of an organic mixed health-care beverage and a preparation method of the organic mixed health-care beverage. The raw materials comprise, by weight, 1-3 g of ginseng, 1-3 g of deer-horn glue, 2-5 g of folium cortex eucommiae, 2-5 g of gynostemma pentaphylla, 2-5 g of hawthorn, 2-5 g of ligusticum wallichii, 1-3 g of schisandra chinensis, 3-5 g of rhizoma polygonati, 2-5 g of radix ophiopogonis, 100-200 mg of algal oil, 200-300 mg of taurine, 150-200 mg of grape seed extract, 3-5 ml of perilla seed oil, 50-80 ml of yoghourt, 30-50 ml of walnut juice and 30-50 ml of peanut liquid. The selenium-enriched beverage is prepared from 30 to 50 milliliters of sweet almond liquid, 30 to 50 milliliters of black sesame liquid, 30 to 50 milliliters of mango juice, 50 to 100 milliliters of apple juice, 30 to 50 milliliters of tomato juice, 50 to 80 micrograms of selenium-enriched yeast, 25 to 50 milligrams of vitamin C, 125 to 25 milligrams of vitamin E, 0 to 15 milligrams of vitamin B1 {thiamine}, 15 milligrams of vitamin B2 {riboflavin}, 0 to 15 milligrams of vitamin B2 {pyridoxine}, 15 milligrams of vitamin B6 {pyridoxine}, 25 milligrams of vitamin B9 {folic acid}, 40 micrograms of vitamin B9 {folic acid}, and 0 to 75 milligrams of vitamin B12 {cobalamin}. After being taken by various people for a period of time, the health-care food is smooth and delicious in taste and free of adverse reaction through evaluation in multiple aspects, and the health-care effect and feeling state of the health-care food are better than those of the prior art. And the health-care tea is suitable for a wide range of people, is beneficial to market promotion, and enables more people to achieve the health-care effect of preventing diseases before diseases.

The invention belongs to the technical field of compound wine, and discloses compound wine capable of improving sleep, enhancing immunity and delaying senescence and a preparation method of the compound wine. 20 traditional Chinese medicine decoction pieces of rhizoma polygonati, radix astragali, radix rehmanniae, American ginseng, dendrobium officinale, cistanche deserticola (desert), folium cortex eucommiae, gastrodia elata, lucid ganoderma, apocynum venetum, gynostemma pentaphylla, fructus rosae laevigatae, folium ginkgo, dogwood, fructus alpiniae oxyphyllae, fructus psoraleae, fructus lycii, semen cassiae, herba leonuri and liquorice are weighed according to the formula proportion and then subjected to water extraction, concentration, alcohol precipitation and concentration to obtain paste, the paste is added into stirred white spirit to be blended into compound wine, and the compound wine is obtained. And after filtering and inspecting to be qualified, filling and packaging. The processing technology is simple in step and convenient to operate, impurities are reduced, nutritional ingredients in the medicinal and edible traditional Chinese medicinal materials are guaranteed, and the tea has unique nutritional value, has the health benefits of improving sleep, enhancing immunity and delaying senescence, and is high in production efficiency and easy to popularize and use.

The invention provides a pharmaceutical composition helpful for reducing blood glucose as well as a preparation and application thereof, and relates to the field of medicines. The pharmaceutical composition helpful for reducing blood glucose comprises the following raw materials in parts by weight: 15-20 parts of pomegranate, 8-13 parts of turmeric, 1-2 parts of bear gall, 1-2 parts of schisandra chinensis, 10-15 parts of dendrobe, 5-10 parts of folium eriobotryae, 8-12 parts of radix puerariae, 5-10 parts of dandelion, 8-15 parts of herba epimedii, 8-12 parts of polygonum cuspidatum, 5-8 parts of gynostemma pentaphyllum, 4-8 parts of inula flower and 3-5 parts of cortex lycii radicis. The pharmaceutical composition disclosed by the invention effectively inhibits sugar absorption of intestinal tracts, enhances insulin sensitivity, improves insulin resistance, promotes activity of glucose transporter protein, regulates glycolipid metabolism balance, inhibits liver glycogen decomposition to reduce liver glucose output and reduces interference of inflammatory factors on insulin signal channels through a multi-channel synergistic effect; therefore, the whole metabolic regulation is optimized, the blood sugar level is remarkably reduced, the number of pancreatic beta cells is increased, and the compound can be applied to preparation of drugs for treating diabetes.

The invention discloses a medicinal and edible dual-purpose formula for reducing hyperlipidemia, improving microcirculation and repairing self-healing power. The invention belongs to the technical field of medicine and food. The formula comprises the following components in parts by weight: 20-30 parts of hawthorn leaves, 25-30 parts of Chinese wolfberry fruits, 30-40 parts of ginkgo leaves, 30-40 parts of black fungus, 30-40 parts of radix puerariae, 20-30 parts of lotus leaves and 30-40 parts of fiveleaf gynostemma herb. The lotus leaves are used for inhibiting fat absorption and promoting lipid metabolism, and the gynostemma pentaphylla is used for regulating blood fat and blood sugar and cooperates with the hawthorn leaves, the black fungus and other original components, so that hyperlipidemia is snipped from multiple links, and the lipid-lowering effect is more remarkable; meanwhile, the raw materials cooperate to create a good physical state, so that the self-healing potential of the body is further stimulated, and the body is assisted to resist diseases and recover health; all the raw materials are medicinal and edible, are natural and mild, have no obvious side effects, are suitable for life cultivation and health preservation or hyperlipidemia adjuvant therapy for a long time, and have huge market potential.

The invention discloses a preparation method of gynostemma pentaphyllum secondary saponins with hypoglycemic activity, and relates to the technical field of preparation of gynostemma pentaphyllum secondary saponins, the preparation method comprises the following steps: mixing gynostemma pentaphyllum total saponins with an ethanol aqueous solution, and preparing a secondary saponin product with hypoglycemic activity by controlling hydrolysis conditions, according to the preparation method of the gynostemma pentaphyllum secondary saponin with the hypoglycemic activity, the hypoglycemic activity of the gynostemma pentaphyllum secondary saponin is remarkably improved by accurately controlling key conditions such as the concentration of the ethanol aqueous solution, the solid-liquid ratio, the hydrolysis temperature and time, the preparation process is simple, convenient, efficient and environmentally friendly, the product purity is high, and the stability is good; meanwhile, the effective components of the product and the good combining capacity of the effective components and diabetes treatment targets are verified through component analysis and molecular docking, scientific basis and technical support are provided for developing novel hypoglycemic drugs or health care products, and the application prospect and market potential are wide.

The invention provides novel application of gypenoside A. Verification shows that the gypenoside A can improve problems caused by cell iron ion accumulation by improving the level of cell iron metabolism related protein, and can be used for preparing medicines for treating diseases caused by iron metabolism disorder, such as neurodegenerative diseases, thalassemia and hemochromia.

The invention mainly relates to a gynostemma pentaphyllum composition as well as a preparation method and application thereof, and belongs to the technical field of biology. The composition comprises 8-12 parts of fiveleaf gynostemma herb, 8-12 parts of barbary wolfberry fruit, 6-10 parts of honeysuckle flower and 3-7 parts of orange. The gynostemma pentaphyllum composition is prepared by the following steps: performing alcohol extraction on gynostemma pentaphyllum, fructus lycii and exocarpium citri rubrum to obtain a solid part, performing water extraction on the solid part to obtain a water extract 1, performing water extraction on honeysuckle to obtain a water extract 2, and mixing the water extract 1 and the water extract 2 to obtain the gynostemma pentaphyllum composition. All the raw materials are compatible with one another and can play a synergistic role, meanwhile, functional components can be better extracted through the extraction method, and then the efficacy of the composition is improved. The composition prepared by the invention can increase the exercise tolerance of the body, improve the metabolic ability to serum urea and lactic acid to adapt to the load state of the body, increase the energy reserve of the body and achieve the effect of effectively relieving physical fatigue.

This invention discloses a method for preparing and applying a Gynostemma pentaphyllum-derived vesicle composition. The preparation method includes: crushing and extracting Gynostemma pentaphyllum with alcohol, followed by enzymatic hydrolysis and stabilization treatment with a combination of enzymes to obtain the Gynostemma pentaphyllum-derived vesicle composition. The preparation method of this invention can obtain Gynostemma pentaphyllum-derived vesicles with clear edges and high uniformity. The obtained Gynostemma pentaphyllum-derived vesicle composition has antioxidant, anti-inflammatory, hair-darkening, and anti-hair loss effects. It retains its complete vesicle structure even after 90 days of storage at room temperature, exhibiting the advantage of vesicle stability throughout the overall shelf life of the raw material and meeting the requirements of cosmetic raw materials. When applied to formulation systems, it does not show significant viscosity reduction, effectively avoiding formulation instability and deactivation.

1. The name of this design product: Packaging box (Hawthorn and Gynostemma pentaphyllum fat-regulating drink). 2. Purpose of this design product: used for packaging of tea substitute beverages. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention belongs to the field of mineral extraction and compound preparation thereof, and particularly relates to an anti-melanin tumor compound containing a pan jade extract and a preparation method of the anti-melanin tumor compound. The compound is prepared from the following components in parts by weight: 20 to 25 parts of pan jade extract, 40 to 50 parts of fiveleaf gynostemma herb extract, 15 to 20 parts of praeruptorin B, 10 to 12 parts of baicalein and 5 to 8 parts of betulin. The preparation method of the compound comprises the following steps: mixing the pan jade extract, the fiveleaf gynostemma herb extract, the praeruptorin B, the baicalein and the betulin in proportion, adding the mixture into absolute ethyl alcohol, stirring for 30 minutes at room temperature, and finally drying at 40-50 DEG C to obtain the melanin tumor resisting compound. According to the anti-melanoma compound provided by the invention, the pan jade extract is modified by adopting the rapeseed polypeptide, and the fiveleaf gynostemma herb extract, the praeruptorin B and the like are scientifically matched, so that a multi-component, multi-target and multi-level synergistic anti-tumor effect is realized, and the defect of serious side effects of traditional chemotherapy is overcome while the melanoma inhibition effect is improved.