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179 results about "Hormone drug" patented technology

Liquid chromatography for synchronously detecting 15 anabolic hormone residues in food

The invention relates to a liquid chromatography for detecting anabolic hormone drug residue in animal-derived food, which is characterized by first sampling: animal musculature is taken off the fat and connective tissue, then minced and evenly ground, and the sample is weighed; extracting: anabolic hormone extract is extracted from the weighed sample by methanol ultrasonic extraction, the extract is evaporated to dryness in water bath through a rotary evaporator, and the residue is dissolved by methanol aqueous solution; purifying: C18 Solidoid extraction column on the extract is carried out solid phase extraction; and testing by devices: the filtrate is tested by opposite phase high efficiency liquid chromatography, quantified by external reference method-peak area, and then carried out with binary gradient elution. In the invention, the detected sample is complex biological sample; the established method can complete one detection in about one hour and is simple and fast, with reliable sensitivity and low cost; the method can analyze more veterinary hormone drug species than the synchronous usage of the existing GC or HPLC methods with easier operation, and has lower cost than the existing GC/MS and LC/MS or LC/MS/MS methods with low solvent toxicity.
Owner:上海国矗生物科技有限公司

Monolithic column, preparation method and application thereof

The invention discloses a monolithic column, a preparation method and a application thereof. The preparation method comprises the following steps: 1) polymeric monomer, pore-foaming agent, initiator and cuprous bromide are mixed together and then added with catalyst ligand after being deoxidized; the mixture is put into a chromatographic column tube to react in a sealing way; after the reaction, the chromatographic column tube is connected with a high pressure transfer pump, and the soluble substances such as the pore-foaming agent and the like are rinsed and removed by organic solvent, so that a monolithic column with a three-dimensional continuous skeleton structure is obtained; 2) the mixture of the organic solution, catalyst, catalyst ligand and antioxidant of the functional monomer is injected into the monolithic column, and bromine group remained on the surface of the column body initiates the surface grafting polymerization reaction of vinyl monomer. The method leads polymerization reaction to be carried out at the room temperature, so as to greatly solves the problems of inhomogeneous column body structure, volume contraction and the like caused by high polymerization temperature. Compared with the traditional monolithic column, the obtained monolithic column has obviously different structure, thus being applicable to separating biological macromolecules such as steroid hormone drugs, protein or the like.
Owner:INST OF CHEM CHINESE ACAD OF SCI

Purification method for progesterone

The invention discloses a purification method for progesterone, belonging to the technical field of preparation and processing of steroid hormone drugs. According to the method, aldehyde impurities ina progesterone crude product react with a sulfur-containing nucleophilic reagent to generate high-polarity hydroxyl sulfonate, the high-polarity hydroxyl sulfonate and progesterone are subjected to solubility differentiation, and then high-purity progesterone with the content higher than 99.5% and the total content of impurity aldehyde lower than 0.2% is obtained through water adding, water precipitation, filtering, filter cake washing and drying. According to the purification method, technical bottlenecks in progesterone purification in the prior art are avoided, all the reagents and raw materials used in the reaction process are low in price and easy to obtain; purification process is easy and convenient to operate, requirements on equipment is low, and cost investment is small; a purification process is mild and safe, conditions are easy to control, and the purification and recovery rate of products is high; the used reagents are low in environmental pollution, and in particular, chromium-containing high-pollution reagents are not used, so the industrial development trend of green chemistry is met; and the method has good economic and social benefits.
Owner:ZHEJIANG SHENZHOU PHARMA

Chinese herbal medicine feed additive for middle and later egg laying periods of ostriches as well as preparation method and application of Chinese herbal medicine feed additive

The invention provides a Chinese herbal medicine feed additive for middle and later egg laying periods of ostriches as well as a preparation method and application of the Chinese herbal medicine feed additive. The additive is prepared into a finished product through smashing and uniformly mixing the following raw materials in parts by weight: 5-15 parts of herba epimedii, 5-15 parts of fructus forsythiae, 4-12 parts of actinolite, 4-12 parts of ocimum gratissimum, 3-9 parts of Chinese yam, 3-9 parts of semen cuscutae, 3-9 parts of pine needle meal, 2-8 parts of glossy privet fruits, 2-8 parts of medicated leaven, 2-8 parts of cowherb seeds, 2-8 parts of astragalus, 2-8 parts of angelica sinensis, 2-6 parts of radix codonopsis, 2-6 parts of leonurus, 2-6 parts of sanguisorba officinalis, 1-5 parts of radix rehmanniae preparata, 1-5 parts of hawthorns and 1-5 parts of malt. The additive simultaneously has a nutritive value and the effects of resisting to stress and viruses, enhancing the immunity of an organism and the like; when the additive is added into a feed to feed ostriches, the egg yield and fertilization rate of ostriches in middle and later egg laying periods can be effectively increased; and the Chinese herbal medicine feed additive is free of residues and toxic or side effects, capable of reducing the usage amount of hormone drugs and favorable in economic benefit and market application prospect.
Owner:SICHUAN AGRI UNIV
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