The invention discloses a medical dressing containing recombinant humanized collagen. The medical dressing consists of the following raw materials in parts by weight: 0.5-1.5% of recombinant humanized collagen, 3-7% of ceramide, 2-6% of nicotinamide, 0.6-1% of medical chitosan, 0.5-1.5% of ginseng radix extracts, 0.2-0.6% of chamomilla extract, 0.3-0.7% of herba portulacae extract, 0.1-0.5% of aloe extract, 0.1-0.3% of natural lactic acid, 0.01-0.05% of polyhexamethylene guanidine, 0.3-0.7% of Carbomer, 3-7% of propylene glycol, 1-5% of glycerol, 0.05-0.15% of triethanolamine and the balance of purified water. The invention also puts forward a preparation method of the medical dressing containing the recombinant humanized collagen. The medical dressing disclosed by the invention has the performance of bacteria resistance, repairing, high biocompatibility and high cell affinity, has the excellent functions of water permeation, ventilation, moisture absorption, wound surface infection reduction and quick lightening of wound pain, has the efficacy of wound surface repairing, aging skin firming and senility resisting, water replenishing, allergy resisting and skin beautifying for the skin of the human body, also can be used for bacteria resisting, cleaning and anti-aging repairing of the female vulva and vagina and is used for accelerating vagina health.