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Vaginal health products

一种阴道、维生素的技术，应用在润湿雌性生殖道以及改善雌性生殖道健康领域，能够解决短暂、不能阴道组织提供长效的作用等问题

Inactive Publication Date: 2010-01-27
KIMBERLY-CLARK WORLDWIDE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of these products is basically water, they can only provide short-term relief of symptoms (1-2 days), and basically can not provide long-term effects on vaginal tissues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] Example 1: Materials and methods

[0136] cell line. CO at 37°C 2 Human cervical epithelial cells (ME-180, ATCC) were propagated and cultured in McCoy's SA medium (Invitrogen) containing 10% heat-inactivated fetal bovine serum (Invitrogen) under gas conditions. The trans-, 9-cis- and 13-cis-retinoic acids used were all purchased from ICNBiomedicals. Retinoic acid stock solutions were prepared in DMSO.

[0137] Toxicity and proliferation assays. Cytotoxicity / proliferation assays were performed as follows. Wash the ME-180 cells with HBSS first, then add 3mL of trypsin (0.25%), and add BDTA and place in a T75 flask for 15 minutes. Add ME-180 cells in trypsin to 7 mL of McCoy's SA medium with 10% FBS. ME-180 cells; centrifuge for 5 minutes and remove supernatant. ME-180 cells were resuspended in 10 mL of McCoy's SA medium with FBS for counting. Centrifuge ME-180 cells for 5 minutes and remove the supernatant. ME-180 cells were resuspended into 6 x 10 4 cells per m...

Embodiment 2

[0157] Example 2: Effect of vitamin A on cervical cell growth and gene expression

[0158] The interaction of ME-180 cervical cells with certain concentrations of vitamin A derivatives was studied to determine whether vitamin A derivatives affect ME-180 cell growth and gene expression.

[0159] Cell proliferation of ME-180 cells. ME-180 cells were incubated with vitamin A derivatives for up to 3 days. Count the number of ME-180 cells every day. The results obtained from these tests are shown in Figures 2 and 3 .

[0160] The data in Figure 1 show that the use of very low concentrations of the vitamin had little effect on cell growth. For example, 0.0 nM (negative control), 1 nM, 10 nM and 100 nM of trans-retinoic acid, 9-cis-retinoic acid or 13-cis-retinoic acid had little effect on cell growth. However, cells exposed to trans-retinoic acid showed faster growth than cells exposed to the other agent or not (unexposed, negative control cells).

[0161] Therefore, another ex...

Embodiment 3

[0168] Example 3: Vitamin A increases mucin-4 expression

[0169] Materials and methods

[0170] RNA was isolated from ME-180 cervical cells treated with OnM or 100 nM vitamin A in the absence of serum (as described above). RNA was reverse transcribed to form DNA, and PCR techniques (as described above) were used to determine the effect of vitamin A on mucin-4 (MUC-4) expression.

[0171] result

[0172] Such as Figure 6 As shown, the addition of the control β-fibrillin initiator resulted in the synthesis of a 300 bp DNA product, which was roughly equivalent in amount across all samples. This result indicated that the amount of template RNA was approximately the same in all samples. However, also as Figure 6As shown, MUC-4 product (800 bp) was found in the cervical cDNA control group (lane 2). In vitamin-free ME-180 cells (lane 3), there was no MUC-4 production. However, after addition of 100 nM vitamin A to ME-180 cells, MUC-4 was detected 4 or 8 hours after treatmen...

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Abstract

The invention provides compositions and methods for increasing the secretion of mucus within the reproductive tract of a female mammal.

Description

technical field [0001] The present invention relates to the use of compounds such as retinoids, carotenoids, nucleotides and nucleosides for moisturizing and improving the health of the female reproductive tract. The compound increases mucus production in vaginal and cervical cells, promotes cell growth and gene expression, and is also used as a contraceptive. Background technique [0002] The secretory function of uterine, cervical, and vaginal mucus cells has important implications for the function and health of the reproductive tract. For example, the quality and quantity of cervical mucus changes during the menstrual cycle, which greatly affects a person's fertility. Under the influence of elevated estrogen levels, the cervical mucus becomes thinner, allowing sperm to pass through. Later in the menstrual cycle, due to increased progesterone levels, the mucus becomes thick and prevents sperm from penetrating, resulting in loss of fertility. The thickening of the cervic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/07A61P15/02A61F6/06A61F13/02A61KA61K31/015A61K31/15A61K31/203
CPCA61K31/07A61K31/203A61K31/15A61P15/00A61P15/02A61P15/18A61K31/015
Inventor J·M·比利亚努埃瓦
Owner KIMBERLY-CLARK WORLDWIDE INC
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