The invention discloses a novel Chinese medicinal composition for treating delayed menstrual cycle and a preparation method thereof. The Chinese medicinal composition mainly comprises the following raw material medicaments: root of red-rooted salvia, szechuan lovage rhizome, safflower, peach seed, roasted pangolin scales, motherwort herb, medlar, tuber fleeceflower root, white paeony root, pilose asiabell root, raw astragalus, cinnamomvine, Chinese angelica, roasted frankincense, roasted myrrh, cassia bark, cassia twig, dried ginger, medcinal evodia fruit, tangerine peel, green tangerine peel, immature bitter orange, cyperusrotundus, szechwan chinaberry fruit, rhizoma corydalis, radix curcumae, suberect spatholobus stem, cowherb seed, radix rehmanniae preparata, donkey-hide glue and the like. The Chinese medicinal composition can be prepared into any one common orally-taken preparation by a conventional Chinese medicinal preparation method. The Chinese medicinal composition can remarkably improve the symptoms: delayed menstrual cycle, delayed menstruation, delayed menstrual cycle, scant menstrual flow, lumpy menses with dark color, lesser abdomen, distention and pain of the breast, depression, lusterless complexion, fatigue and lack of strength and other symptoms, and has the advantages of extract clinical treatment effect, remarkable treatment effect, quick response, basically no toxic or side effect and the like.