The invention provides a pure traditional Chinese medicine composition for treating female sterility, comprising the following medicines by parts by weight: 12-20 parts of Chinese angelica, 5-10 parts of Szechuan lovage rhizome, 8-10 parts of stir-fried white paeony root, 12-20 parts of raw rehmannia root, 8-10 parts of red ginseng, 8-10 parts of stir-fried largehead atractylodes rhizome, 6-10 parts of peppermint, 5-10 parts of liquoric root, 8-10 parts of Chinese thorowax root, 3-6 parts of largetrifoliolious bugbane rhizome, 5-8 parts of baical skullcap root, 6-10 parts of Indian buead, 8-10 parts of hiraute shiny bugleweed herb, 6-10 parts of tree peony bark, 15-25 parts of motherwort herb, 5-10 parts of tangerine peel, 5-10 parts of stir-fried nutgrass galingale rhizome, 15-25 parts of danshen root, 12-20 parts of India madder root, 15-25 parts of membranous milkvetch root, 12-20 parts of Chinese wolfberry root-bark, 8-10 parts of yanhusuo, 8-10 parts of stir-fried combined spicebush root, 8-10 parts of starwort root, 15-25 parts of cuttlefish bone, 8-10 parts of cape jasmine fruit, 8-10 parts of blackend swallowwort root, 8-10 parts of rose, 8-10 parts of Chinese rose flower and 15-25 parts of tangerine leaf. The invention further provides a pure traditional Chinese medicine preparation prepared thereby. The pure traditional Chinese medicine composition has the effects of warmly nourishing liver and kidney, benefiting qi, nourishing blood, promoting blood circulation, removing blood stasis, dissipating phlegm, removing dampness, dispersing stagnated liver qi and relieving qi stagnation and can effectively treat irregular menstruation, amenorrhea, lower abdominal pain, hectic fever and sterility; moreover, the pure traditional Chinese medicine preparation has simple preparation, low production cost and convenient administration.