The invention discloses discloses a formula and a production method of ganoderma lucidum qi-benefiting essence-generating wine. Ganoderma lucidum, dodder, rosa laevigata, actinolite, epimediums, radix rehmanniae preparata, morinda officinalis, cynomorium songaricum, eucommia ulmoides, desertliving cistanche, fructus corni, astragalus, rhizoma curculiginis, and deer horn glue are used as main materials. The production method of the wine mainly includes the technological processes of material selecting, drying, dispensing, steeping, packaging and product finishing and the wine has the effects of nourishing the heat, soothing nerves, nourishing the kidney, strengthening yang, benefiting qi, generating essence, tonifying the liver, nourishing the spleen and the like. In addition, according to the formula and the production method of the ganoderma lucidum qi-benefiting essence-generating wine, the ganoderma lucidum qi-benefiting essence-generating wine is delicious in taste, convenient to use, purely natural, free of poison and side effects, and suitable for being drunk in restaurants and houses.