11 results about "Rhododendron dauricum" patented technology

The invention belongs to the technical field of organic synthesis and medicinal chemistry, and discloses a total synthesis method of rhododendron heteroterpenin Rhododaurines A-C. The total synthesis method comprises the following steps: carrying out allyl stereoselective addition on a compound 3 and a compound 5 in a toluene solvent under the conditions of catalysis of n-butyllithium and low temperature of-78 DEG C, and constructing a chiral center to obtain a compound 6; removing a methoxy methyl (MOM) protecting group from the compound 6 under an acidic condition, cyclizing, and reacting to generate rhododaurine C (7); the rhododaurine C (7) is subjected to intramolecular [4 + 2] cycloaddition and oxidation reaction in the illumination and oxygen atmosphere of 300-385 nm, and rhododaurine B (8) and an epoxy epimer epinedodaurine B (9) are obtained; and carrying out protonation and rearrangement on the epidodaurine B (9) by virtue of acid catalysis cyclic ether, so as to generate the rhododaurine A (10). The method can overcome the defects that the content of rhododendron miscellaneous terpenoids Rhododaurines A-C in nature is extremely low, and large-scale extraction is difficult.

The application discloses a kind of miraculin compounds and its extraction method and application, belong to the field of traditional Chinese medicine extraction, specifically related to a kind of miraculin compounds as shown in general formula (I) and (II) isolated from Rhododendron dauricum and the extraction method of the compound, and the compound or the isomer of the compound, or the inhibitory effect of the compound on inflammation in pharmaceutically acceptable salt or pharmaceutical composition comprising the compound can be used for preparing anti-inflammatory drugs.The application further enriches the structural diversity of active substances of Rhododendron dauricum, which lays a foundation for subsequent related biological activity tests of monomeric compounds, provides active lead compounds for new drug development, and also provides a theoretical basis for in-depth research and development of Rhododendron dauricum medicinal materials.

The invention discloses a Rhododendron mucronatum callus induction in-vitro regeneration method. The method comprises the following steps: selecting a stem segment of a sterile sowing seedling as an explant; inoculating the explant into a callus induction culture medium, culturing for 30 days under a dark condition, and then culturing under an illumination condition; transferring the calluses into a differential culture medium, continuously culturing under an illumination condition, and inducing the adventitious buds to differentiate, so as to obtain regenerated plants. According to the method, through explant design, in combination with a callus induction culture medium and a differentiation culture medium of a specific formula and accurately controlled culture conditions, the induction rate of the rhododendron erythrophyllum callus reaches 96.67%, the browning rate is only 4%, the differentiation rate of the adventitious buds reaches 71.11%, more than 63 adventitious buds can be differentiated from each callus block on average, the rooting rate reaches 63.33%, and the survival rate of the rhododendron erythrophyllum callus is greatly improved. Reliable technical support is provided for large-scale production and genetic improvement of Rhododendron mucronatum.

The invention discloses a rhododendron dauricum twig cutting propagation method, and belongs to the technical field of plant propagation. According to the method, a complete rhododendron dauricum twig cuttage system is formed through cooperation of cutting slip treatment, matrix bearing, environment regulation and control and final-period management, precisely treated cutting slips take roots in a proper cuttage bed matrix, a greenhouse system provides a stable temperature and humidity environment, continuous growth of seedlings is guaranteed through staged management, and the survival rate of the seedlings is increased. Finally, three core objectives are achieved: firstly, the cuttage survival rate is increased (greater than or equal to 90%), and the problem of low traditional propagation efficiency is solved; 2, good characters of stock plants are stably reserved, and character separation of seed propagation is avoided; large-scale and standardized production of seedlings is achieved, high-quality seedlings are provided for ecological protection, landscape architecture and industrial development, and sustainable utilization of rhododendron dauricum resources is promoted.

The invention relates to the technical field of plant breeding, and discloses an oriental cherry rhododendron cutting seedling raising method and application thereof.The method comprises the steps of S1, facility and substrate preparation, specifically, seedling raising is conducted in a greenhouse, and Kanuma soil is selected as a cutting substrate; s2, cutting preparation: from mid-to-late May to early-to-mid September, selecting annual branches with semi-lignified upper parts and no plant diseases and insect pests of a rhododendron simsii plant as cuttings, cutting the cuttings into cuttings with the length of 10-15cm, reserving leaves on the cuttings, performing cutting seedling raising of the rhododendron simsii in a greenhouse, preparing an advanced monitoring system and a control system, and performing cutting seedling raising of the rhododendron simsii in the greenhouse. Air temperature and humidity, soil temperature and humidity and illumination intensity can be accurately monitored in real time, the cuttage method provides an effective way for vegetative propagation, excellent characters of stock plants can be reserved, meanwhile, direct digging of wild oriental cherry rhododendron in the cold highland area can be reduced through manual cuttage propagation, local fragile ecological balance can be maintained, and the survival rate of the wild oriental cherry rhododendron in the cold highland area is increased. And a virtuous circle of protection and utilization is realized.

The invention relates to a carbon-methyltransferase (RdCMT) and an application of the carbon-methyltransferase (RdCMT) in synthesis of farrerol. RdCMT is cloned and is subjected to heterologous expression in Escherichia coli BL21 (DE3) by mining transcriptome information of Rhododendron dauricum, the generated fusion protein of which the N terminal or / and the C terminal are / is connected with tags can specifically and efficiently catalyze C-3 'and C-5' methylation of chalcone compounds naringenin chalcone to generate a double-carbon-methylation product, and the double-carbon-methylation product is spontaneously dehydrated to generate the farrerol. The invention also relates to the application of the RdCMT in a model plant Nicotiana benthamiana, and the production of the farrerol through the construction of a total synthesis path of the Nicotiana benthamiana.

The present invention relates to the field of microbial technology, and in particular to a strain of Pezicula ericae XA4 and its application. The present invention provides a strain of Pezicula ericae XA4, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on June 26, 2024, with a deposit number of CGMCC No.41350. The XA4 provided by the present invention was back-grafted on aseptically cultured Dahurian rhododendron seedlings, and trypan blue staining was used to detect that mycorrhizal fungi could be back-grafted successfully. Compared with the uninoculated strain, the inoculation of XA4 had the most significant growth-promoting effect on Dahurian rhododendron. Its plant height, leaf length, leaf width, root length, biomass, chlorophyll content, net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, and root activity were all improved compared with the uninoculated strain.

The invention relates to a preparation method of compound antibacterial volatile oil sustained-release granules, which comprises the following steps: firstly, extracting pine needle volatile oil from pine needles by combining a liquid nitrogen quick-freezing method and a microwave extraction method; secondly, extracting folium rhododendri daurici volatile oil from folium rhododendri daurici plants by adopting an enzyme-assisted hydrogel distillation method; then embedding the pine needle volatile oil and the rhododendron dauricum volatile oil into the sustained-release particles by a complex coacervation method; and finally, carrying out performance tests on the sustained-release particles, including embedding rate determination and volatilization rate comparison experiments, so as to verify the sustained-release and antibacterial properties of the sustained-release particles. Results of comparison of volatilization rates and bacteriostatic effects of pure volatile oil and sustained-release particles show that the prepared compound volatile oil sustained-release particles have significant advantages in the aspects of volatile oil release delaying and bacteriostatic action. The method provides a new preparation scheme for the slow release system of the compound antibacterial volatile oil, and has a better application prospect.

The invention discloses a process for extracting folium rhododendri daurici essential oil by synergistically promoting hydro-distillation through glucose oxidase, and belongs to the technical field of folium rhododendri daurici essential oil extraction.The process comprises the following steps that S1, folium rhododendri daurici leaves are checked, impurities, worm eggs and moldy leaves are removed, and the folium rhododendri daurici leaves are ground and sieved; s2, adding a proper amount of sterile ddH2O, uniformly mixing, adding an enzyme preparation, and performing water bath; s3, after the water bath is finished, distilling and extracting the rhododendron dauricum essential oil. According to the process for extracting the rhododendron dauricum essential oil by synergistically promoting the hydro-distillation through the glucose oxidase, through mutual cooperation of various enzymes, the dissolution rate and the extraction rate of the rhododendron dauricum essential oil can be greatly increased, the content of oxygen-containing compounds in effective components-volatile components in the rhododendron dauricum essential oil is increased, and then the utilization rate of the rhododendron dauricum is increased; the method is green and efficient, consumes short time, does not use an organic solvent, and does not affect subsequent research and application; the method has low requirements on equipment, is simple and easy to operate and can be used for industrial production.

The invention discloses application of a folium rhododendri daurici and hippophae rhamnoides leaf composition in preparation of a medicine for treating psoriasis. Belongs to the technical field of traditional Chinese medicine compositions. The invention provides application of a combination of rhododendron dauricum and hippophae rhamnoides leaves in preparation of a medicine for treating psoriasis, it is found for the first time that the combination of rhododendron dauricum serving as a main medicine and hippophae rhamnoides leaves serving as an adjuvant according to the mass ratio of (1-5): 1 is better in psoriasis treatment effect compared with single rhododendron dauricum or hippophae rhamnoides leaves, the skin lesion condition and PASI score of the back of a mouse with psoriasis can be remarkably reduced, and the application prospect is broad. According to the present invention, the psoriasis treatment effect is substantially improved, the psoriasis mouse epidermal thickness is reduced, the mouse spleen swelling condition is relieved, the spleen index is reduced, the psoriasis related inflammatory factor IL-17 and TNF-alpha content in the mouse serum is reduced, the psoriasis treatment effect is substantially improved, and the research results show that the dahurian rhododendron leaf and the hippophae rhamnoides l leaf have the synergistic effect on psoriasis treatment, the treatment effect of the compound is superior to that of a positive drug methotrexate, and a new direction is provided for psoriasis treatment.

This invention discloses a low-toxicity aqueous extract of Rhododendron dauricum, its preparation method, and its applications, belonging to the field of natural plant extract preparation and detoxification technology. Addressing the difficulty in selectively removing toxic components from Rhododendron dauricum aqueous extracts, this invention provides a detoxification method based on membrane separation technology. This method involves obtaining the total aqueous extract of Rhododendron dauricum, using membrane separation technology to directionally remove toxic enriched fractions with molecular weights between 5 and 10 kDa, and then recombining, concentrating, and drying the remaining components to obtain the low-toxicity aqueous extract. Zebrafish embryo toxicity experiments verified that the low-toxicity aqueous extract obtained by this invention significantly increased the median lethal concentration (LD50) compared to the untreated total aqueous extract, and significantly alleviated developmental toxicity phenotypes such as pericardial edema. This invention features a mild process, no solvent residue, high selectivity, and good reproducibility. While retaining the main effective substances, it significantly improves the safety and controllability of Rhododendron dauricum preparations, and has broad industrial application prospects.