265 results about "Mouse Spleen" patented technology

The invention discloses a human SARS-CoV-2 monoclonal antibody. The preparation method of the human SARS-CoV-2 monoclonal antibody comprises the steps: adopting SARS-CoV Nucleocapsid recombinant protein as immunogen, immunizing BALB / c mice, performing fusion and subcloning on spleen cells and myeloma cells of mice, then performing a large amount of repeated screening and domestication of cell lines through commercialized products SARS-CoV-2 Nucleocapsid and MERS Nucleocapsid so as to obtain a hybridoma cell line capable of secreting the SARS-CoV-2-resistant N monoclonal antibody with high affinity and high specificity finally and successfully, and finally performing ascites preparation and purification so as to obtain the monoclonal antibody, wherein the amino acid sequence of the SARS-CoVNucleocapsid recombinant protein is shown in SEQ ID No. 1. The invention also discloses application of the monoclonal antibody in preparation of SARS-CoV-2 virus detection products and preparation ofdrugs for inhibiting the SARS-CoV-2 viruses. The monoclonal antibody can be used for detecting the SARS-CoV-2 in human throat swabs / pulmonary secretions and other samples by using a double-antibody sandwich method, and can be applied to diagnosis and prevention and control of SARS-CoV-2 virus infection and scientific researches of viruses and other study.

The invention belongs to the technical field of genetic engineering, and in particular relates to screening of a single-chain antibody against fumonisin and application thereof in immunoassay of fumonisin. The screening method comprises the following steps of: directly setting off from coupled antigen FB1-KLH(keyhole limpet hemocyanin) immunized mouse spleen cells, establishing a single-chain antibody gene library by a molecular cloning method and technology, then screening by a phage display technology, expressing ELISA(enzyme-linked immuno sorbent assay) test, sequencing, and finally obtaining the single-chain antibody against fumonisin named as FB-Mu 1H3 with high affinity and a coding gene thereof. The single-chain antibody can be directly applied to assay of fumonisin after being expressed and purified in a large quantity in escherichia coli, and the applications include assay of fumonisin pollution in field crops, feed, grain or food.

The invention belongs to the technical field of biomedicines, and in particular relates to application of seaweed polysaccharides. The seaweed polysaccharides can be taken as preparations for preparing anti-viral or immune medicines. Cell models and in-vivo animal experiments show that the seaweed polysaccharides can be used for significantly enhancing the animal immunity, and can also be used for promoting the generation of cell factors, the typing of T lymphocytes and the proliferation of mouse spleen cells so as to ensure that cellular immune reaction can be activated. The functional seaweed polysaccharides disclosed by the invention are natural polysaccharides which are extracted from seaweeds such as large seaweeds and kelps, gulfweeds, grateloupia sparsa, eucheuma muricatum, ulva pertusa kjellm, enteromorpha, gracilaria, ascophyllum nodosum and scytosiphon lomentaria, and can also be low-molecular-weight seaweed polysaccharides or seaweed oligosaccharides which are prepared by degrading the seaweed polysaccharides by virtue of different preparation methods. According to the application disclosed by the invention, a polysaccharide extract of single seaweed or a polysaccharide extract mixture of a variety of seaweeds can be taken as a novel anti-viral and immune enhancing agent to be applied to feeds of livestock, poultry, fish, shrimps and shellfish, and has wide application prospects.

A medicine combination has the function of resisting lung cancer. The combination is mixed with rhizoma paridis saponin and milk vetch root amylose according to the weight ratio of 3 to 1. The rhizoma paridis is extracted by alcohol and then the rhizoma paridis extract is obtained through gradient elutriation of macroporous absorption resin alcohol; the milk vetch root is extracted by water and then the protein is settled and removed to get the milk vetch root amylose; the two extracts are mixed and the medicine is made. The MTT activities experiment in vitro with the MTT method has proven that the medicine combination can obviously control the growth of various lung cancer cells such as LA795 lung adenocarcinoma cell and the IC50 can reach 26.73 ug / ml; the experiment of mouse with the lung cancer tumor has represented that the tumor constraint rate can reach 55.63 percent; in this way, lung transfer of hypodermic transplanted tumor of the mouse with the lung cancer tumor can be obviously controlled and the tumor cells can be brought to death; moreover, spleen index and thymus index can be promoted and the medicine is innocuous and has no side effect. In addition, compared with the raw materials, the medicine combination has high activity and clear function; moreover, the medicine can be made into different types.

The invention discloses a GPC3 (glypican-3) monoclonal antibody hybridoma cell strain 7D11 and a preparation method and application thereof, which belong to the technical field of bioengineering. The GPC3 monoclonal antibody hybridoma cell strain 7D11 has a preservation code of CGMCC No.5426. The preparation method of the hybridoma cell strain 7D11 includes: using GPC3 protein as an immunogen to immunize a mouse, subjecting spleen cells of the mouse with serum titer more than 1:104 and myeloma cells SP2 / 0 to fusion, using a HATRPMI-1640 medium to screen fused cells, screening by ELISA (enzyme-linked immuno sorbent assay) and multiple limiting dilution process, and finally obtaining the hybridoma cell strain 7D11. The GPC3 monoclonal antibody hybridoma cell strain 7D11 is high in yield of secreted antibodies and easy to survive, the secreted monoclonal antibodies are high in titer, flexible in reaction, easy to detect, low in production cost and widely applicable to detection of GPC3 protein expression.

The invention discloses a preparation method and application of a product obtained by coupling melamine with carrier protein, as well as a melamine antibody. The product obtained by coupling the carrier protein with the melamine is used as artificial antigen and applied to an immunological method for melamine detection. The preparation method comprises the following steps of immunizing animals with the coupled product so as to prepare an antibody used for melamine detection, fusing BALB / C mouse spleen cells immunized with the coupled product and SP2 / 0 mouse myeloma cells, obtaining hybridoma capable of stably transferring culture and secreting anti-melamine specific monoclonal antibodies by screening positive hybridoma and cloning cells, and preparing an ascites monoclonal antibody. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the melamine, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling melamine with carrier protein, as well as the melamine antibody provides service for the rapid detection of melamine-type residue in foods.

The invention relates to a Ganoderma leucocontextum new strain and a cultivation method thereof. The Ganoderma leucocontextum new strain is Ganoderma leucocontextum HMGIM-Z110122 of which the preservation number is CCTCC (China Center For Type Culture Collection) NO:M2016087. The cultivation method of the Ganoderma leucocontextum CCTCC NO:M2016087 comprises the following steps: manufacturing a mother strain after the tissue isolation of the strain, manufacturing a stock culture, manufacturing a production strain, and carrying out cultivation and Ganoderma leucocontextum growth management. The Ganoderma leucocontextum new strain and the cultivation method thereof have the beneficial effects that the invention provides the Ganoderma leucocontextum new strain and the corresponding manual cultivation method. Ganoderma leucocontextum is rare at present, can effectively accelerate the proliferation of mouse spleen cells, is high in nutrition value and has good economic benefit and social benefit.

The invention relates to the field of protein engineering, in particular to a method for preparing CysC-paired monoclonal antibodies, which comprises the following steps of: screening hybridoma cells having specific binding with CysC after fusing mouse splenic cells and myeloma cells capable of generating CysC antibodies, preparing cells for generating the monoclonal antibodies, finishing first-time cell fusion, further purifying out the monoclonal antibodies, and selecting one monoclonal antibody to be marked with horse radish peroxidase; then implementing the second-time fusion of the mouse splenic cells and the myeloma cells; finishing CysC-antigen coating on an elisa plate, adding a hybridoma-cell cultural supernatant obtained through the second-time cell fusion to elisa-plate holes, arranging a blank control hole, subsequently adding the monoclonal antibody marked with the horse radish peroxidase to the elisa-plate holes, incubating at the temperature of 37 DEG C for 30 minutes, adding a substrate to each hole for color rendering, and measuring a light absorption value under 450nm after stopping reaction through sulfuric acid; and selecting the uninhibited holes as the paired monoclonal antibodies.

Provided herein are a hybridoma cell line producing monoclonal antibody against foot-and-mouth disease virus (FMDV), the monoclonal antibody therefrom, reagent and kit for ELISA, and immunoassay method. The hybridoma cell line is produced by cell fusion of a parental cell and a myeloma cell line and has the same characteristics as the cell line whose strain designation is CmA40 and deposition number is ATCC (To be Provided). The parental cell is a splenocyte isolated from the spleen of a mouse immunized by an antigen derived from a 3ABC non-structural protein (NSP) of FMDV. The antigen used here is expressed by a prokaryotic cell. The monoclonal antibody produced by the hybridoma cell line can specifically recognize a 3ABC polypeptide and does not cross-react with an antiserum of swine vesicular disease virus.

The invention belongs to microbiological animal cell system fiels, which in detail relates to a pancreatic cancer hepatic metastases clone SW1990HM with hepatic metastases potence. The clone possesses special transition phenotype, and if inoculated to mouse spleen, it will result to 100% hepatic metastases, and it is the first human pancreatic cancer clone with hepatic metastases potence in domestic. The genetic backgroud of said clone strain is the same to that of origianl cell, and it is characterized by strong invasiveness, high transmission efficiency and large transmission range. The invention provides proper experimental platform for further researching, preventing and treating pancreatic cancer hepatic metastases.

The invention provides a preparation method and application of a product obtained by coupling penicillin with carrier protein, as well as a beta-lactam type penicillin antibody. Animals are immunized with penicillin artificial antigen coupled in the invention so as to prepare the antibody which can be used for detecting beta-lactam type penicillin in foods. The preparation method comprises the following steps: immune BALB / C mouse spleen cells and SP2 / 0 mouse myeloma cells are fused; beta-lactam type antibiotics coupled with the carrier protein are used as coating antigen to screen positive hybridoma; hybridoma capable of stably transferring culture and secreting anti-beta-lactam type antibiotic antibodies through cell clones is obtained; and an ascites monoclonal antibody is prepared. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the beta-lactam type antibiotics, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling penicillin with carrier protein, as well as the beta-lactam type antibiotic antibodies can serve the rapid detection of beta-lactam type antibiotic residue in foods.

The invention relates to a hybridoma cell strain secreting thiamethoxacin monoclonal antibody and application thereof, belonging to the field of food safety immunodetection. The accession number of the hybridoma cell strain is CGMCC No. 14699. According to the invention, a complete Freund's adjuvant is used for primary immunization of a BALB / c mouse, then an incomplete Freund's adjuvant is used for booster immunization three times, and a thiamethoxam complete antigen containing no adjuvant is used for impact immunization once, so the BALB / c mouse is immunized; and then the high-titer low-IC50spleen cells of the immunized mouse are fused with mouse myeloma cells by using a PEG method, and then the cell strain is obtained through indirect competitive ELISA screening and subcloning three times. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (with an IC50 value of 0.81 ng / mL) to thiamethoxam and can be used for detection of thiamethoxamresidues in food.

The invention relates to an ELISA (enzyme-linked immunosorbent assay) method for quickly testing C-Myc by using a monoclonal antibody. According to the method, prokaryotic expression C-Myc protein is purified through protein purification technology; immune mouse spleen cells are performed cell fusion with SP2 / 0 myeloma cell, and a clone capable of stably secreting an antibody against C-myc IgG1 and a Cmyc IgM antibody clone are obtained based on ELISA screening and the subtype identification of subclone and monoclonal antibody Ig. A sandwich ELISA method and the two direct ELISA methods for quickly testing c-Myc are built by using an antibody against c-Myc IgG1 and an antibody against c-Myc Ig. With the method, a C-myc protein sample is accurately detected, the detected C-myc protein concentration range is 0.01-1000 pmol / L, and the detection limit is 0.01-0.05 pmol / L; the specificity and the sensitivity of C-Myc detection are greatly improved, the operation is simple, and the repeatability is high; and the method detects the expression level of C-Myc in tumor tissues, and provides experimental data for screening of malignant tumor.

The invention relates to a morinda root water extract, coarse polysaccharides and oligosaccharide and polysaccharide components and preparation methods and application thereof. The morinda root waterextract and the coarse polysaccharides are extracted from morinda roots by a mode of water extraction, then the coarse polysaccharides are separated to obtain the oligosaccharide and polysaccharide components in the coarse polysaccharides, and properties are measured. Experiments show that both the morinda root water extract and the coarse polysaccharides provided by the invention can promote theproliferation of spleen cells and the secretion of cell factors in mice, promote the proliferation of human liver cells, reduce the damage of toxic agents on cells, inhibit the expression of hepatitisB surface antigens and core antigens, inhibit the proliferation of liver cancer cells as well as inhibit the damage of ConA to the livers and kidneys of the mice, have obviously better biological activity than that of the morinda root oligosaccharides, polysaccharides or morinda root polysaccharide components prepared by other methods, and have the prospect of being developed into immunomodulators, anti-liver injury and anti-tumor drugs or health-care food. Preparation steps are simple, and preparation processes have less pollution to the environment and are suitable for industrial production.

The invention relates to an imidacloprid pesticide against monoclonal antibody and a preparation method thereof, and belongs to the technical field of biology. The preparation method comprises the following steps of: immunizing a BALB / c mouse by using a coupling substance of immune hapten 1-[6-(2-carboxyethylsulfenyl-3-pyridine) methyl]-N-nitro-2-imidazoline imine and bovine serum albumin, preparing hybrid tumor cells from spleen cells and myeloma cells Sp2 / 0 of the immunized mouse by the hybrid tumor technology, and obtaining hybrid tumor strains 2F11 / A9 capable of stably secreting the imidacloprid pesticide against monoclonal antibody. By effectiveness verification, the antibody can be used for sensitive and quick detection of imidacloprid residues in agricultural production environments and agricultural products. The preparation technique for the imidacloprid pesticide against monoclonal antibody is simple and feasible, does not need special instruments and equipment in the whole preparation process of the antibody, and is easy for scale production in factories.

The recombinant staphylococcus aureus enterotoxin M as one kind of superantigen with the amino acid sequence as shown in SEQ ID No. 1 is obtained through recombining gene originated from staphylococcus aureus and coding SEM with one kind of plasmid vector, transforming to proper host for expressing, and affiliation purifying. It is proved through extracorporeal mouse spleen lymphopoiesis experiment and tumor cell inhibiting experiment that the recombinant protein has typical superantigen activity stronger than that of SEC and may be applied in preparing medicine for stimulating lymphopoiesis and inhibiting tumor cell growth. The present invention is suitable for preparing high purity enterotoxin with superantigen activity and developing superantigen preparation. The present invention has reasonable design, efficient expression on recombinant SEM in the expression strength of about 15-25 % of staphylococcus aureus protein, and other advantages.

The invention relates to a human body beta-amyloid protein detection kit and application thereof. The human body beta-amyloid protein detection kit is prepared by the following steps: firstly, immunizing a mouse by use of antigen beta-amyloid precursor protein, separating out obvious lymph nodes in the spleen and the body of the mouse after confirming successful immunization, extracting total RNAs (Ribose Nucleic Acids), performing reverse transcription on the total RNAs to obtain cDNAs (complementary Desoxvribose Nucleic Acids), and amplifying the light and heavy chain genes of an antibody by virtue of PCR (Polymerase Chain Reaction); secondly, assembling and amplifying scFvDNA and constructing pCANTAB5E-scFv recombinant plasmid; thirdly, converting the plasmid to obtain a single-chain antibody phage library, and screening out to obtain target antibodies; and finally, assembling the target antibodies to form the human body beta-amyloid protein detection kit. The detection range of the screened antibodies is relatively wide from 0 to 1,000pg / ml, the linear correlation coefficient of the kit is high, the detection sensitivity of the kit can be 3.5pg / ml, and the kit achieves high stability; during detection, the reaction patterns of a two-step method and a room temperature oscillating method can be adopted, with low requirements on the laboratory conditions and the equipment.

The invention discloses a coupling product of streptomycin and carrier protein, and a preparation method of a streptomycin antibody and an application thereof, which relate to a coupling method of the carrier protein such as keyhole limpet hemocyanin, human serum albumin, cow serum albumin, ovalbumin and the like with streptomycin, and immune BALB / C mouse spleen cells are fused with SP2 / 0 mouse myeloma cells by streptomycin immunogens coupled with the carrier protein, and the streptomycin coupled with the carrier protein is used as a coating antigen for screening positive hybridoma and hybridoma that can steady passage and excrete anti-specificity streptomycin monoclonal antibody can be obtained by the cell clone, and ascites monoclonal antibody is prepared. The prepared monoclonal antibody is used for building a direct competition ELISA method with high specificity, sensitivity and accuracy to the streptomycin and immunity colloidal gold test strips. The coupling of the streptomycin and the carrier protein and the preparation method of the streptomycin monoclonal antibody can provide services for detecting streptomycin residue in foods quickly.

The invention discloses a method for preparing an antihuman recombinant tissue factor monoclonal antibody. The method comprises the following steps: combining a truncated human recombinant tissue factor obtained by purification after prokaryotic expression with a freund adjuvant immunized mouse; fusing mouse spleen cells with myeloma cells; selectively cultivating and screening an HAT selective medium; after the subcloning cultivation is subjected to limiting dilution, selecting target hybridoma cells by an ELISA method, performing massive proliferation, and injecting into an abdominal cavity of a syngeneic mouse to prepare ascites; and obtaining the antihuman recombinant tissue factor monoclonal antibody which has excellent potency and higher yield. The antihuman recombinant tissue factor monoclonal antibody prepared by the method of the invention has the advantages of high sensitivity and strong specificity, can be used for immunoaffinity purification of the human recombinant tissue factor, and also can be used for research and development of anticoagulant medicaments and medicaments for treating tumors.

The invention discloses a Pb<2+> antigen and a corresponding monoclonal antibody, and also provides a method for preparing the same. The preparation method comprises the following steps of: chelating Pb<2+> with a chelating agent p-NH2-Bn-CHX-A''-DTPA; coupling glutaraldehyde with hemocyanin (KLH); performing ultra-filtration to obtain a full immunizing antigen (KLH-NH-Bn-CHX-A''-DTPA-Pb); merging splenic cells and myeloma cells of a mouse immunizing BALB / c; and screening a specific monoclonal antibody which has strong positive for BSA-NH-Bn-CHX-A''-DTPA-Pb and no cross reaction with BSA-NH-Bn-CHX-A''-DTPA and other various metal ions. The antibody can be used for developing ELISA kits and test paper for detecting the Pb<2+> and has great application prospect.

The invention relates to a porcine pseudorabies virus (PRV) resisting hybridoma cell line, a preparation method thereof, a monoclonal antibody and an application thereof. The preparation method of the porcine pseudorabies virus resisting hybridoma cell line specifically comprises the following steps of: (1) preparing an antigen immune BALB / c mouse by using PRV; (2) fusing immune mouse spleen cells with SP2 / 0 myeloma cells; and (3) screening out hybridoma cells secreting PRV resisting monoclonal antibodies. In the invention, high-purity PR antigens are obtained through purification by an Sepharose4FF chromatographic column, the screening of subsequent positive clones and the generation of antibodies are guaranteed, an indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) method is determined to quickly determine the generation and the titer of antibodies, and a lot of RPV antibodies are obtained by inoculating BALB / c female mouse enterocoelia. The antibody is a single antibody aiming RP antigens, has the advantages of strong specificity, high purity, good homogeneity and the like and can be used for diagnosing and treating PR. Meanwhile, certain technical reference is provided for the preparation of other types of antibodies.

The invention discloses a triadimefon monoclonal antibody hybridoma cell strain B11S and application thereof, and belongs to the technical field of food safety immunodetection. The triadimefon monoclonal antibody hybridoma cell strain B11S disclosed by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC), is classified and named as a monoclonal cell strain,is preserved on October 14, 2019, and has a preservation number of CGMCC No. 18518. A BALB / c mouse is immunized by a triadimefon complete antigen. High-titer and low-IC50 mouse splenocytes are takenand fused with myeloma cells through a PEG method, and the hybridoma cell strain B11S is obtained through screening of an indirect competitive enzyme-linked immunosorbent assay and three times of subcloning. A monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (IC50 value is 5.25 ng / mL) to triadimefon, provides a raw material for immunodetection of triadimefon residues in foods, and has practical application value.

The invention relates to an anti-bifenthrin monoclonal antibody and an application thereof and especially provides a monoclonal antibody capable of specifically recognizing bifenthrin, a hybrid tumor generating the monoclonal antibody and the application of the monoclonal antibody. The invention belongs to the field of biology. A method includes following steps: immunizing BALB / c female mice by an artificial antigen; performing cell fusion with myeloma cell SP2 / 0 and mice spleen cells to prepare a hybridoma; obtaining a hybridoma strain which can stably secret the anti-bifenthrin monoclonal antibody; injecting the hybridoma strain into abdomen of the mice to prepare ascites; purifying the ascites in an octanoic acid-ammonium sulfate manner to obtain the specific anti-bifenthrin specifically monoclonal antibody. The monoclonal antibody does not cross-react with other compounds. ELISA method established by the antibody can be used for quickly, sensitively, conveniently and cheaply detecting residual bifenthrin in environment and agricultural products.

The invention relates to an anti-thiacloprid monoclonal antibody and an application thereof, and specifically relates to a monoclonal antibody specifically recognizing thiacloprid and an application of the monoclonal antibody. The invention relates to the field of biology. According to the invention, an artificial antigen is used for immunizing a BALB / c female mouse; myeloma cells SP2 / 0 are subjected to cell fusion with mouse spleen cells, such that hybridoma cells are prepared; through series screening and sub-cloning, a hybridoma cell strain stably secreting the anti-thiacloprid monoclonal antibody is obtained; the hybridoma cell strain is injected into a mouse through intra-abdominal injection, such that ascite fluid is prepared; the mouse ascite fluid is purified with an octanoic acid-ammonium sulfate method, such that the thiacloprid specific monoclonal antibody is prepared. The antibody does not cross react with other compounds. An enzyme-linked immuno-chromatographic analysis method established with the antibody can be used in detecting environment and agricultural product thiacloprid residue rapidly, sensitively and conveniently with a low cost.

The invention relates to a mimic eptitope peptide of BAFF-R and a DNA coding the peptide and the application of the mimic eptitope peptide in the preparation of antitumor bacterins and drugs. The mimic eptitope of 7 amino acids of BAF-R is a mimic eptitope of molecule BAFF-R with high affinity with a monoclonal antibody of BAFF-R, and is obtained through selection from a phage random display 7-peptide bank with a monoclonal antibody of BAFF-R as the antigen, wherein the sequence of the amino acids is Gly Tyr Thr Arg Trp Gly Cys. The 7-amino-acid mimic eptitope can also be used to construct a poly-peptide vaccine. The clonal inhibition rates of the phage display peptide provided by the invention are all more than 50 percent, and can specifically inhibit the combination between antibody and antigen for a larger extent, greatly improve the proliferation of mouse spleen lymphocytes without adjuvant, the mimic antigen is good in immunogenicity, as illustrated by the induced cell immune response after mice are vaccinated with the mimic antigen.

The invention discloses a GPC3 (glypican-3) monoclonal antibody hybridoma strain 8G6, and a preparation method and application thereof, which belong to the technical field of cell engineering. The preservation code of the GPC3 monoclonal antibody hybridoma strain 8G6 is CGMCC (china general microbiological culture collection center) No.5427. The preparation method of the GPC3 monoclonal antibody hybridoma strain 8G6 includes: using GPC3 protein as immunogen to immunize a mouse; and fusing mouse splenocytes having serum titer more than 1:104 with SP2 / 0 myeloma cells, using an HATRPMI-1640 medium to screen fusion cells, and finally obtaining the hybridoma strain 8G6 by ELISA (enzyme-linked immunosorbent assay) and repeated limiting dilution. The GPC3 monoclonal antibody hybridoma strain 8G6 is high in yield of secretory antibodies, and is easy to survive, and the secreted monoclonal antibodies are high in titer, sensitive in reaction, easy to detect, low in production cost and widely applicable to detection of GPC3 protein expression.

The invention discloses a polyene high-oxidation androstane compound, i.e., androstane-4, 6, 8 (9), 13 (14)-tetraene-3, 11, 16-triketone. The compound is extracted from epigynum auritum plant. Experiments prove that the compound has an obvious suppression effect on Balb / c mouse spleen lymphopoiesis stimulated by ConA (concanavalin) and has activity equivalent to that of the conventional immunosuppression active substances, i.e., triptolide and dexamethasone.

The invention discloses porcine reproductive and respiratory syndrome virus M protein CTL cell epitopes and application thereof. The identification of the epitopes comprises the following steps of: fusing and cloning a M protein gene of a porcine reproductive and respiratory syndrome virus CH-1a strain and a mouse ubiquitin gene to form a DNA vaccine and also form recombination viruses, expressing an M protein, of a WR strain vaccinia virus; immunizing a BALB / c mouse according to a Priming-Boosting policy, separating the mouse splenic lymphocyte, culturing and stimulating a CTL short peptide in vitro predicted and synthesized by a bioinformatic method, and using the flow cytometry and enzyme-linked immunospot technology to identify two CTL epitopes that are K93FITSRCRL and F57GYMTFVHF. The identification of the PRRSVM protein CTL cell epitopes lays a certain theoretical foundation for the PRRS cell immune mechanism and the novel epitope pepetide vaccine and has an instructing significance for the theoretical study on PRRS preventing and control technology.

The invention provides divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing a medicine and functional food for immune adjustment and tumor resistance. According to the invention, the prepared divaricate velvetplant root and rhizome polysaccharide can promote mononuclear macrophage strain to phagocytose neutral red and release nitric oxide, can promote B cell proliferation and T cell proliferation, can also obviously improve immunosuppressive mouse spleen and thymus indexes, can improve the transformation and the proliferation of T cells and B cells, can increase the level of immunosuppressive mouse white blood cells, can obviously restrain the growth of tumor tissues, can protect organisms, can restrain the decline of weight, can gain weight, and can obviously restrain the migration of epidermic cells in veins. In addition, polysaccharide is the main functional component of divaricate velvetplant roots and rhizomes, has various health-care efficacies, is derived from purely natural materials, does not have any side effects, and can be developed into medicines and health-care products for immune adjustment, tumor resistance and angiogenesis resistance, so that the divaricate velvetplant root and rhizome polysaccharide has favorable market application prospects.

The invention discloses novel skeleton dalesconols, which has a general formula described at the right; wherein, 1,3,5,7,9,11,13,15,17,18,19,21,23,25,27 and 29 are the numbers of carbon atomic ordinal, R<1> is H or OH, when R<1> is H, dalesconols is dalesconols A; and when R<1> is OH, dalesconols is dalesconols B. The dalesconols has strong competitive inhibition to the mouse spleen cell proliferation caused by Con A, and has no toxicity to the normal mouse spleen cells; thus the dalesconols can be used as the compound with the functions of immunity and inhibition, and also can be applied to the preparation of the relevant novel medicine.