884 results about "Zebrafish" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention provides a zebra fish multi-target tracking method and system based on a space science experiment video. The method comprises the following steps: firstly, obtaining a zebra fish space science experiment video; then, motion information is extracted based on the zebra fish space science experiment video, and the motion information is used for representing a motion state represented by pixel points in the zebra fish space science experiment video; then, multi-modal feature fusion is carried out based on the motion information and the appearance information, and multi-modal fusion features are determined; and finally, target detection and tracking are carried out according to the multi-modal fusion features, and target movement track information corresponding to the target zebra fishes is determined. According to the method, motion feature information representing dynamic changes is extracted through motion modeling based on a self-adaptive threshold value, then the interaction and fusion relation between appearance and motion features is modeled step by step through a heterogeneous graph, and the robustness of target detection, data association and trajectory prediction is enhanced. Therefore, the stability and the accuracy of multi-target tracking of the zebra fish can be effectively improved.

The invention discloses a lactobacillus paracasei SX-M1559 strain and application thereof in preparation of drugs for reducing blood sugar and lipid and resisting inflammation, and belongs to the technical field of microorganisms. The invention discloses a lactobacillus paracasei SX-M1559, and the preservation number of the lactobacillus paracasei SX-M1559 is CCTCC (China Center For Type Culture Collection) NO: M 2025030. According to the lactobacillus paracasei SX-M1559 and the application thereof in preparation of the medicine for reducing blood sugar, blood fat and inflammation, the SX-M1559 is separated from fresh feces of long-life old people, teai county, Zhanjiang city, Guangdong province, the glucose content in a zebra fish hyperglycemia model body and the fat content in a zebra fish hyperlipidemia model body can be remarkably reduced, and the effect of reducing blood sugar, blood fat and inflammation is achieved. The invention provides a theoretical reference and a guidance basis for developing a probiotic preparation for preventing or treating hyperlipidemia, diabetes mellitus and inflammatory bowel disease by utilizing the new strain SX-M1559 of the casei paracasei.

The invention provides a structure, a preparation method and an application method of a group of anti-wrinkle compact cyclopeptide compounds. The anti-wrinkle compact cyclic peptide compound comprises an anti-wrinkle cyclic peptide-1 with a sequence of c (CDLARLAMYA), and / or an anti-wrinkle cyclic peptide-2 with a sequence of c (KTFKKTFK). The promotion rates of the anti-wrinkle cyclopeptide-1 to zebra fish collagen gene expression col1a1a, col1a1b and col1a2 under the concentration of 0.001% are respectively 156%, 87% and 103%. The promotion rate of the cyclopeptide on zebra fish elastin Elna gene expression under the test concentration of 0.001% is 132%. Under the concentration of 0.001%, the promotion rates of the anti-wrinkle cyclopeptide-2 on zebra fish collagen gene expression col1a1a, col1a1b and col1a2 are 94%, 84% and 66% respectively. The promotion rate of the cyclopeptide on zebra fish elastin Elna gene expression under the test concentration of 0.001% is 66%.

The invention belongs to the technical field of cosmetic efficacy evaluation, and discloses a sunscreen efficacy evaluation method and application thereof, and the method comprises the following steps: building a blank group; establishing a model group; establishing a sample group; performing constant-temperature incubation on the blank group, the model group and the sample group for a first period of time, recording a swimming track, and calculating swimming distance increment and after-sun burning pain relieving efficiency of each group of zebrafish; performing constant-temperature incubation on the blank group, the model group and the sample group for a second duration, recording the tail fin and dorsal fin area of the zebra fish, and calculating the sunburn prevention efficiency; performing constant-temperature incubation on the blank group, the model group and the sample group for a third period of time, recording the head melanin area of the zebra fish, and calculating the anti-sunburn efficiency; and grading the sunscreen effect of the sample. The method disclosed by the invention has the advantages of low cost, short test period, high reproducibility, high visualization degree, intuitive and understandable test result and the like, and can be used for accurately grading the sunscreen effects of chemical sunscreen agents, water-soluble sunscreen cosmetics and raw materials thereof from the whole animal level.

The invention provides a polypeptide which is derived from zebra fish antibacterial peptide and is improved in heat resistance. The amino acid sequence of the polypeptide AR10 is WKRKRWRIWK, and the polypeptide AR10 has higher antibacterial activity than the unmodified polypeptide AR10, and has better high temperature resistance, salt resistance and serum resistance. The antibacterial peptide provided by the invention not only has a good antibacterial effect on common Gram-positive and Gram-negative pathogenic bacteria, but also has a good antibacterial effect on clinically common drug-resistant bacteria, and cell experiment results show that the screened antibacterial peptide has biological safety. Therefore, the antibacterial peptide provided by the invention has broad-spectrum antibacterial property and high biological safety, and can be used for developing antibacterial and bacteriostatic products related to human bodies.

The invention belongs to the technical field of probiotics, and particularly relates to lactobacillus reuteri ShuiMian with a sleep aiding effect and application of the lactobacillus reuteri ShuiMian. A new ShuiMian strain is separated from feces of a healthy infant in Guangzhou city, Guangdong province, PTZ is adopted to construct a zebra fish insomnia model, and research finds that the strain can inhibit eye movement frequency, inhibit brain discharge, reduce dopamine secretion, promote GABA secretion and promote Gabra gene expression, and the zebra fish insomnia model can be used for treating insomnia. The effect of promoting sleep is achieved. Therefore, the strain has a unique sleep-promoting mechanism, can assist in developing novel sleep-promoting medicines in the field of medicines, and provides a safer and more effective treatment scheme for insomnia patients. In the health care industry, a nutritional supplement can be prepared, the mass sleep quality is improved, and the development of sleep health related industries is promoted.

The invention discloses a strain of Lactobacillus paracasei SX-G1520 and an application of the Lactobacillus paracasei SX-G1520 in preparation of a lipid-lowering, anti-inflammatory and / or antioxidant product, the preservation number of the Lactobacillus paracasei SX-G1520 is CCTCC (China Center for Type Culture Collection) No: M 2025032, and the Lactobacillus paracasei SX-G1520 is preserved in the China Center for Type Culture Collection on January 6, 2025. The Lactobacillus paracasei SX-G1520 strain provided by the invention has lipid-lowering and antioxidant functions, significantly reduces the content of intestinal neutrophils of a zebra fish inflammatory bowel disease model, shows a significant effect on relieving intestinal inflammation, is good in safety, and has good application prospects. And a new technical choice is provided for developing probiotic preparations for regulating fat metabolism, resisting oxidative stress and preventing and treating inflammatory bowel diseases.

The invention discloses an eel antibacterial polypeptide Ajaspkidin and application thereof in preparation of an anti-vibrio composition. The amino acid sequence of the eel antibacterial polypeptide Ajaspkidin is shown as SEQ ID NO.01. The invention further discloses an application of the eel antibacterial polypeptide The zebra fish antibacterial peptide has the characteristics of excellent molecular characteristics, broad-spectrum antibacterial activity, efficient bactericidal ability, high thermal stability, high biological safety, anti-infection effect on zebra fish infected by vibrio vulnificus, capability of remarkably improving the survival rate of bred animals and the like, and has a wide application prospect.

The invention belongs to the technical field of biological medicine, and particularly relates to application of chlorbuconazole or salt thereof in preventing, relieving or treating diseases related to HIF-2alpha. In-vitro experiments prove that the chlorbuconazole enhances the transcriptional activity of HIF-2alpha so as to promote the expression of downstream genes regulated and controlled by the HIF-2alpha; it is proved that chlorburazol has the effects of increasing erythropoiesis and relieving renal injury on an aristolochic acid-induced zebra fish chronic kidney disease model, a cisplatin-caused acute kidney injury and renal anemia model and a unilateral ureteral obstruction model. Meanwhile, the traditional Chinese medicine composition belongs to new use of old medicines, so that the medicine research and development cost and the treatment cost are saved, and the traditional Chinese medicine composition has obvious economic benefits and clinical application values.

The invention belongs to the technical field of biology, and particularly relates to milk-derived pentapeptide for promoting calcium mineral absorption and application of the milk-derived pentapeptide. The CPP containing the high-activity peptide fragment is obtained by optimizing a process, and the influence of the CPP on mineral absorption is verified by utilizing a zebra fish model. Then, the pentapeptide GPFPI with calcium absorption promoting activity is screened out from the CPP in combination with peptidomics and a molecular docking technology, functional verification is carried out, it is found that the pentapeptide presents a dose-dependent trend on Ca < 2 + > transport, the Ca < 2 + > transport amount is remarkably increased by 23.23% at 10 mu M, and meanwhile, zebra fish experiments show that intervention of the GPFPI effectively reverses accumulated optical density value reduction caused by dexamethasone, and the effect of inhibiting calcium absorption is achieved. Compared with a manufacturing module, the skull accumulative optical density value of a GPFPI (2-20 mu M) processing group is remarkably increased. It is shown that the synthetic peptide GPFPI can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention provides an application of WNT7B in construction of a myopia animal model. Homologous genes wnt7ba and wnt7bb of the WNT7B in the zebra fish are knocked out or knocked down by applying a CRISPR / Cas9 gene editing technology, an animal model of which the eye axis length is remarkably increased, the eyeball movement frequency is remarkably reduced and the light response is reduced is obtained, and the result shows that the WNT7B is related to the high myopia. The model provides a favorable tool for pathogenesis and drug screening of high myopia, and has a good application prospect.

The invention belongs to the technical field of probiotics, and particularly relates to fermented lactobacillus mucus BM01 with an effect of relaxing bowel and application of the fermented lactobacillus mucus BM01. A new fermented lactobacillus mucus BM01 strain is separated from excrement of a healthy infant in Guangzhou city, Guangdong province, a zebra fish constipation model is constructed through loperamide hydrochloride, and research finds that the strain can remarkably promote intestinal tract movement, increase the content of in-vivo serotonin and inhibit the enzyme activity of dipeptidyl peptidase-4, so that the constipation of the zebra fish can be inhibited, the constipation of the zebra fish can be inhibited, and the constipation of the zebra fish can be inhibited. Therefore, the bowel relaxing effect is achieved. It is indicated that the BM01 strain can play the bowel relaxing effect through multiple mechanisms, has significant application value, can be used for research and development of drugs for treating constipation, irritable bowel syndrome and other intestinal diseases or development of health care products with the bowel relaxing effect, and can also be used as a feed additive to improve animal intestinal health and reduce disease risks. The culture benefit is improved.

The invention discloses a double-recognition fluorescent molecular probe, a preparation method thereof and application of the double-recognition fluorescent molecular probe in detection of lead ions and / or glyphosate, and relates to the technical field of detection of lead ions and pesticide glyphosate. The double-recognition fluorescent molecular probe disclosed by the invention is a Schiff base type probe which takes dicyanoisophorone and benzoyl hydrazine as fluorophores. The invention also relates to application of the double-recognition fluorescent molecular probe in lead ion sensing detection. The complex of the double-recognition fluorescent molecular probe and lead ions can be used for sensing detection of glyphosate. The double-recognition fluorescent molecular probe provided by the invention has the advantages of easily available raw materials and simple operation. According to the present invention, the quantitative detection of the lead ions / or the glyphosate by the probe through the different functions displayed by the ultraviolet and fluorescence spectra is achieved, the reversibility is strong, the multi-time cyclic utilization can be achieved, the selectivity is good, the influence by other metal ions is not easily generated, the detection limit is low, and the excellent application imaging is provided in animal bodies such as cells, zebra fish and the like.

The invention relates to the technical field of pesticides, in particular to a validamycin / thifluzamide double-loaded preparation with an acid-base response characteristic. The preparation method comprises the following steps: firstly, loading thifluzamide by using calcium carbonate to form a microsphere structure, and simultaneously loading validamycin and thifluzamide calcium carbonate microspheres by using sodium carboxymethyl cellulose and sodium alginate as carrier materials to construct the double-load pesticide preparation. The validamycin / thifluzamide double-loaded preparation provided by the invention has the characteristic of responding to the pH value of the environment, and can simultaneously realize quick release of validamycin and long-acting release of thifluzamide, so that the pesticide effect is remarkably improved. Besides, the validamycin / thifluzamide double-loaded preparation provided by the invention has good leaf surface wettability and release performance, has a relatively good prevention effect on rice sheath blight disease, and has relatively high safety to aquatic organisms represented by zebra fish.

The invention discloses a blood protein precise active peptide capable of tonifying qi and blood, improving microcirculation and promoting vasodilation and a preparation method and application thereof, and belongs to the technical field of blood protein peptide. The blood protein precision active peptide comprises at least one of LGFP, IGFP, FPHFN, LFL, LFI, FESF, ILF, LIF, FLL, FIL, FII or FPHFD. The prepared blood protein precision active peptide has multiple biological activities: an antihypertensive effect is achieved by inhibiting ACE and vasoconstriction substances; vasodilation is promoted by activating a PI3K / AKT / eNOS signal channel; a zebra fish model proves that the traditional Chinese medicine composition has the effects of remarkably improving microcirculation and tonifying qi and blood. The preparation method is low in equipment requirement, simple in process, easy to operate and convenient for industrial large-scale production. The method enriches a bioactive peptide library, and is of great significance in promoting technical progress and industrial upgrading in the field of hemoglobulin peptides.

The invention provides a dilated cardiomyopathy zebrafish model and a construction method and application thereof, and belongs to the technical field of biology.The dilated cardiomyopathy zebrafish model is constructed by overexpressing miR-16-5p in myocardial cells of zebrafish, and the sequence of miR-16-5p is shown as SEQ ID NO.1; the invention further provides a construction method of the dilated cardiomyopathy zebra fish model. The invention constructs the dilated cardiomyopathy zebrafish model of overexpression miR-16-5p in myocardial cells for the first time, and the dilated cardiomyopathy zebrafish model can be used for screening drugs for resisting dilated cardiomyopathy.

The invention discloses a whitening active peptide with an effect of inhibiting melanin formation as well as a preparation method and application of the whitening active peptide. The amino acid sequence of the whitening active peptide is as follows: YYYDPWAGK. Whitening activity verification is carried out on the peptide, the relative tyrosinase activity in cells can be remarkably reduced to 164.26 + / -3.03% from 245.52 + / -1.55% by the peptide with the concentration of 200 mu g / mL, and meanwhile, the relative melanin content can be reduced to 130.05 + / -1.23% from 206.65 + / -4.33%. In addition, the peptide not only can significantly reduce the relative tyrosinase activity of zebrafish from 100 + / -0.36% to 60.42 + / -0.73%, but also can reduce the relative melanin content of zebrafish from 100 + / -2.01% to 63.74 + / -2.05%, and lays an important theoretical foundation for the research and application of zebrafish in the fields of functional food, health care products, cosmetics and medicines.

The invention provides application of active decapeptide in preparation of a medicine for preventing or treating cerebral apoplexy, and belongs to the technical field of biological medicine, the active decapeptide with the amino acid sequence as shown in SEQ ID NO.1 is applied to prevention or treatment of cerebral apoplexy for the first time, the active decapeptide can effectively inhibit formation of cerebral thrombosis of zebra fish with ischemic cerebral apoplexy, and the active decapeptide can be used for preventing or treating cerebral apoplexy. The brain blood flow supply is recovered; meanwhile, the active decapeptide can significantly reduce the hemorrhagic area and hemorrhagic rate of the zebra fish brain with hemorrhagic stroke, repair brain vascular injury and inhibit the occurrence and development of hemorrhagic stroke. In addition, the active decapeptide has small toxic and side effects, and is of great significance for improving the clinical curative effect of cerebral apoplexy and reducing the medication risk when being used for research and development of novel drugs for resisting cerebral apoplexy.

The invention provides collagen tripeptide as well as a preparation method and application thereof, and belongs to the technical field of active peptide. The invention provides a preparation method of collagen tripeptide, which comprises the following steps: carrying out enzymolysis treatment on tilapia scales and high-temperature-resistant protease, then carrying out enzymolysis treatment by using alkaline protease and neutral protease in sequence, filtering enzymatic hydrolysate by using a ceramic membrane and an organic filter membrane in sequence, and intercepting by using a 400Da nanofiltration membrane to obtain collagen tripeptide. The permeate contains active peptide mainly composed of collagen tripeptide. After the permeate is concentrated and dried, the collagen tripeptide powder is detected, and the protein content reaches 96.2%, the GPH content is 3.8%, and the CTP content is 35.09%. The collagen tripeptide powder has better DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity; the traditional Chinese medicine composition has an obvious ROS removing effect, a beta-galactosidase activity inhibiting effect and a telomerase activity enhancing promoting effect on a hydrogen peroxide induced zebrafish aging model, and shows an obvious anti-aging effect.

The invention discloses a lactobacillus mucus fermentation strain and application thereof, the lactobacillus mucus fermentation strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on February 21, 2025, and the preservation number is GDMCCNO: 65936. The feruloyl esterase is separated and screened from white sour soup in Kailing in southeast of Guizhou province, has feruloyl esterase activity, and shows good blood fat reducing and fatty liver treating capabilities in a HepG2 high-fat cell model and a high-fat zebra fish model. The compound has a good prospect in the development of products for preventing or treating hyperlipidemia and fatty liver and relieving obesity.

The invention discloses a zebra fish behavior shooting system and method, and relates to the technical field of image processing, and the system comprises a camera which is arranged at the top of a box body; the experiment module is used for culturing zebra fish, and a camera shoots images of the experiment module and the zebra fish; and the computer is used for detecting the edge of the experiment module in the image, taking an area enclosed by the edge as an activity area of the zebra fish, identifying the position of the zebra fish in the activity area, and connecting the positions to form a track diagram of the zebra fish. The application has remarkable advantages in compatibility, and can adapt to various different types of pore plates and self-defined experiment modules. Through flexible hardware configuration and modular design, the system can be customized and adjusted according to experiment requirements, limitation of traditional equipment during experiment switching is avoided, and larger experiment freedom degree and applicability are provided for researchers.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention provides an antibacterial peptide derived from zebra fish, the amino acid sequence of the antibacterial peptide is SEQ ID NO: 1, and the sequence of coding nucleotide of the antibacterial peptide is SEQ ID NO: 2. The antibacterial peptide provided by the invention can be used for preparing products for preventing, treating and resisting bacteria. The zebrafish antibacterial peptide mP60-81 disclosed by the invention has a remarkable antibacterial effect on gram-positive bacteria and gram-negative bacteria, is wide in antibacterial spectrum, has great use value, and has better application in the aspect of developing and preparing antibacterial agents. The antibacterial peptide is derived from zebra fish, can be applied to aquaculture as a feed additive, and can also be developed into an antibacterial agent, an antibacterial drug and the like, so that the antibacterial peptide has a wide application prospect.

The invention discloses a siraitia grosvenorii instant coffee beverage assisting in reducing uric acid and a preparation method thereof, and belongs to the technical field of food based on the theory of homology of medicine and food. The coffee beverage is composed of instant coffee powder, a compound sweetening agent, coconut milk powder, milk powder and composite herbal powder in a specific proportion. The compound herbal powder is composed of poria cocos, peach kernels, dried tangerine or orange peel and momordica grosvenori which are selected from a medicinal and edible substance catalog. The herbal active ingredients and a coffee system are deeply fused by optimizing an extraction process, the dual regulation effects of inhibiting xanthine oxidase and promoting uric acid excretion are synergistically exerted, and the technical contradiction that common caffeine possibly affects uric acid excretion is effectively solved. The zebra fish in-vivo experiment proves that the level of uric acid can be remarkably reduced (Plt: 0.001), the improvement rate is as high as 17.63%, and a safe and effective daily diet intervention scheme is provided for people with hyperuricemia while the coffee flavor and convenience are provided.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention discloses a high-flux zebra fish behavior analysis device and method, and aims to solve the problem of compatibility of plate edge distortion and a multi-type pore plate, a telescopic mechanism is arranged in a shell of the device, an isolation cavity is formed in the shell when the telescopic mechanism is retracted, a micro-lens array and a light filter plate are sequentially arranged in the isolation cavity from top to bottom, and the micro-lens array and the light filter plate are arranged in the shell. A stimulation module is arranged at the bottom of the light filter plate, a pore plate placement area is arranged between the stimulation module and the light filter plate, and a universal adaptive bracket for accommodating a standard pore plate and a non-standard pore plate is arranged in the pore plate placement area; a camera module comprising at least one high-frame-rate camera with a fixed focal length is correspondingly arranged above the porous plate placing area, the camera module is connected with a control and analysis module, distortionless track diagrams, heat diagrams and behavior parameters are output online, and the optical mechanical integrated distortion correction and porous plate combined universal adaptation structure is used for correcting the distortion of the porous plate. When the same set of light path is switched between standard plates and non-standard plates of different models, the distortion of the edge view field is automatically compensated.

The invention provides a construction method and application of a dilated cardiomyopathy animal model, belongs to the technical field of biological medicine and animal genetic model construction, and provides a novel dilated cardiomyopathy zebrafish model which is constructed by specifically overexpressing a truncated dnajb6b gene long isomer fragment in zebrafish myocardial cells. The nucleotide sequence of the gene segment is as shown in SEQ ID NO. 1; the invention also provides a construction method and application of the dilated cardiomyopathy zebra fish model, and further discovers application of 4-phenylbutyric acid in preparation of drugs for treating dilated cardiomyopathy.

The invention provides a control method, device and equipment for preparation of hypotonic nutritious food and a storage medium, and relates to the technical field of preparation control of nutritious products. The method comprises the following steps: obtaining a measured osmotic pressure corresponding to a mixed material of the to-be-prepared hypotonic nutritious food; the measured osmotic pressure is obtained by measuring the osmotic pressure of a material sample of the mixed material based on a freezing point method; inputting the measured osmotic pressure into the trained linear regression analysis model to obtain a predicted value of the peristalsis promotion rate; the model is obtained based on milk powder reconstituted fluid osmotic pressure and zebra fish intestine peristalsis promotion rate training. Determining a preparation process optimization scheme according to the predicted value of the peristalsis promotion rate and the early warning condition of the peristalsis promotion rate, and adjusting process parameters according to the preparation process optimization scheme; wherein the process parameters in the optimization scheme of the preparation process comprise one or more of the content range of different types of proteins, the ratio of exogenous calcium to milk-derived calcium, the homogenizing pressure and the spray drying temperature. According to the method, functional evaluation can be rapidly completed, and preparation parameters can be optimized.

The invention provides a prinsepia utilis royle extract as well as a preparation method and application thereof, and the preparation method comprises the following steps: picking prinsepia utilis royle seeds, removing epicarp from the prinsepia utilis royle seeds, dehydrating to obtain dried kernels, preparing prinsepia utilis royle crude oil from the kernels by a subcritical extraction method, refining the crude oil by a deodorization vacuum process to obtain refined non-winterized prinsepia utilis royle oil, and drying the refined non-winterized prinsepia utilis royle oil to obtain the prinsepia utilis royle extract. And performing cold tank winterization treatment on the refined non-winterized prinsepia utilis royle oil to obtain the refined winterized prinsepia utilis royle oil. The prinsepia utilis royle extract obtained by the embodiment of the invention has good effects of resisting oxidation, inhibiting tyrosinase, inhibiting hyaluronidase, inhibiting elastinase, repairing zebra fish and the like.