721 results about "Zebrafish" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention provides a zebra fish multi-target tracking method and system based on a space science experiment video. The method comprises the following steps: firstly, obtaining a zebra fish space science experiment video; then, motion information is extracted based on the zebra fish space science experiment video, and the motion information is used for representing a motion state represented by pixel points in the zebra fish space science experiment video; then, multi-modal feature fusion is carried out based on the motion information and the appearance information, and multi-modal fusion features are determined; and finally, target detection and tracking are carried out according to the multi-modal fusion features, and target movement track information corresponding to the target zebra fishes is determined. According to the method, motion feature information representing dynamic changes is extracted through motion modeling based on a self-adaptive threshold value, then the interaction and fusion relation between appearance and motion features is modeled step by step through a heterogeneous graph, and the robustness of target detection, data association and trajectory prediction is enhanced. Therefore, the stability and the accuracy of multi-target tracking of the zebra fish can be effectively improved.

The invention belongs to the technical field of cosmetic efficacy evaluation, and discloses a sunscreen efficacy evaluation method and application thereof, and the method comprises the following steps: building a blank group; establishing a model group; establishing a sample group; performing constant-temperature incubation on the blank group, the model group and the sample group for a first period of time, recording a swimming track, and calculating swimming distance increment and after-sun burning pain relieving efficiency of each group of zebrafish; performing constant-temperature incubation on the blank group, the model group and the sample group for a second duration, recording the tail fin and dorsal fin area of the zebra fish, and calculating the sunburn prevention efficiency; performing constant-temperature incubation on the blank group, the model group and the sample group for a third period of time, recording the head melanin area of the zebra fish, and calculating the anti-sunburn efficiency; and grading the sunscreen effect of the sample. The method disclosed by the invention has the advantages of low cost, short test period, high reproducibility, high visualization degree, intuitive and understandable test result and the like, and can be used for accurately grading the sunscreen effects of chemical sunscreen agents, water-soluble sunscreen cosmetics and raw materials thereof from the whole animal level.

The invention belongs to the technical field of probiotics, and particularly relates to lactobacillus reuteri ShuiMian with a sleep aiding effect and application of the lactobacillus reuteri ShuiMian. A new ShuiMian strain is separated from feces of a healthy infant in Guangzhou city, Guangdong province, PTZ is adopted to construct a zebra fish insomnia model, and research finds that the strain can inhibit eye movement frequency, inhibit brain discharge, reduce dopamine secretion, promote GABA secretion and promote Gabra gene expression, and the zebra fish insomnia model can be used for treating insomnia. The effect of promoting sleep is achieved. Therefore, the strain has a unique sleep-promoting mechanism, can assist in developing novel sleep-promoting medicines in the field of medicines, and provides a safer and more effective treatment scheme for insomnia patients. In the health care industry, a nutritional supplement can be prepared, the mass sleep quality is improved, and the development of sleep health related industries is promoted.

The invention discloses an eel antibacterial polypeptide Ajaspkidin and application thereof in preparation of an anti-vibrio composition. The amino acid sequence of the eel antibacterial polypeptide Ajaspkidin is shown as SEQ ID NO.01. The invention further discloses an application of the eel antibacterial polypeptide The zebra fish antibacterial peptide has the characteristics of excellent molecular characteristics, broad-spectrum antibacterial activity, efficient bactericidal ability, high thermal stability, high biological safety, anti-infection effect on zebra fish infected by vibrio vulnificus, capability of remarkably improving the survival rate of bred animals and the like, and has a wide application prospect.

The invention belongs to the technical field of biological medicine, and particularly relates to application of chlorbuconazole or salt thereof in preventing, relieving or treating diseases related to HIF-2alpha. In-vitro experiments prove that the chlorbuconazole enhances the transcriptional activity of HIF-2alpha so as to promote the expression of downstream genes regulated and controlled by the HIF-2alpha; it is proved that chlorburazol has the effects of increasing erythropoiesis and relieving renal injury on an aristolochic acid-induced zebra fish chronic kidney disease model, a cisplatin-caused acute kidney injury and renal anemia model and a unilateral ureteral obstruction model. Meanwhile, the traditional Chinese medicine composition belongs to new use of old medicines, so that the medicine research and development cost and the treatment cost are saved, and the traditional Chinese medicine composition has obvious economic benefits and clinical application values.

The invention belongs to the technical field of biology, and particularly relates to milk-derived pentapeptide for promoting calcium mineral absorption and application of the milk-derived pentapeptide. The CPP containing the high-activity peptide fragment is obtained by optimizing a process, and the influence of the CPP on mineral absorption is verified by utilizing a zebra fish model. Then, the pentapeptide GPFPI with calcium absorption promoting activity is screened out from the CPP in combination with peptidomics and a molecular docking technology, functional verification is carried out, it is found that the pentapeptide presents a dose-dependent trend on Ca < 2 + > transport, the Ca < 2 + > transport amount is remarkably increased by 23.23% at 10 mu M, and meanwhile, zebra fish experiments show that intervention of the GPFPI effectively reverses accumulated optical density value reduction caused by dexamethasone, and the effect of inhibiting calcium absorption is achieved. Compared with a manufacturing module, the skull accumulative optical density value of a GPFPI (2-20 mu M) processing group is remarkably increased. It is shown that the synthetic peptide GPFPI can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention provides an application of WNT7B in construction of a myopia animal model. Homologous genes wnt7ba and wnt7bb of the WNT7B in the zebra fish are knocked out or knocked down by applying a CRISPR / Cas9 gene editing technology, an animal model of which the eye axis length is remarkably increased, the eyeball movement frequency is remarkably reduced and the light response is reduced is obtained, and the result shows that the WNT7B is related to the high myopia. The model provides a favorable tool for pathogenesis and drug screening of high myopia, and has a good application prospect.

The invention belongs to the technical field of probiotics, and particularly relates to fermented lactobacillus mucus BM01 with an effect of relaxing bowel and application of the fermented lactobacillus mucus BM01. A new fermented lactobacillus mucus BM01 strain is separated from excrement of a healthy infant in Guangzhou city, Guangdong province, a zebra fish constipation model is constructed through loperamide hydrochloride, and research finds that the strain can remarkably promote intestinal tract movement, increase the content of in-vivo serotonin and inhibit the enzyme activity of dipeptidyl peptidase-4, so that the constipation of the zebra fish can be inhibited, the constipation of the zebra fish can be inhibited, and the constipation of the zebra fish can be inhibited. Therefore, the bowel relaxing effect is achieved. It is indicated that the BM01 strain can play the bowel relaxing effect through multiple mechanisms, has significant application value, can be used for research and development of drugs for treating constipation, irritable bowel syndrome and other intestinal diseases or development of health care products with the bowel relaxing effect, and can also be used as a feed additive to improve animal intestinal health and reduce disease risks. The culture benefit is improved.

The invention relates to the technical field of pesticides, in particular to a validamycin / thifluzamide double-loaded preparation with an acid-base response characteristic. The preparation method comprises the following steps: firstly, loading thifluzamide by using calcium carbonate to form a microsphere structure, and simultaneously loading validamycin and thifluzamide calcium carbonate microspheres by using sodium carboxymethyl cellulose and sodium alginate as carrier materials to construct the double-load pesticide preparation. The validamycin / thifluzamide double-loaded preparation provided by the invention has the characteristic of responding to the pH value of the environment, and can simultaneously realize quick release of validamycin and long-acting release of thifluzamide, so that the pesticide effect is remarkably improved. Besides, the validamycin / thifluzamide double-loaded preparation provided by the invention has good leaf surface wettability and release performance, has a relatively good prevention effect on rice sheath blight disease, and has relatively high safety to aquatic organisms represented by zebra fish.

The invention discloses a blood protein precise active peptide capable of tonifying qi and blood, improving microcirculation and promoting vasodilation and a preparation method and application thereof, and belongs to the technical field of blood protein peptide. The blood protein precision active peptide comprises at least one of LGFP, IGFP, FPHFN, LFL, LFI, FESF, ILF, LIF, FLL, FIL, FII or FPHFD. The prepared blood protein precision active peptide has multiple biological activities: an antihypertensive effect is achieved by inhibiting ACE and vasoconstriction substances; vasodilation is promoted by activating a PI3K / AKT / eNOS signal channel; a zebra fish model proves that the traditional Chinese medicine composition has the effects of remarkably improving microcirculation and tonifying qi and blood. The preparation method is low in equipment requirement, simple in process, easy to operate and convenient for industrial large-scale production. The method enriches a bioactive peptide library, and is of great significance in promoting technical progress and industrial upgrading in the field of hemoglobulin peptides.

The invention provides a dilated cardiomyopathy zebrafish model and a construction method and application thereof, and belongs to the technical field of biology.The dilated cardiomyopathy zebrafish model is constructed by overexpressing miR-16-5p in myocardial cells of zebrafish, and the sequence of miR-16-5p is shown as SEQ ID NO.1; the invention further provides a construction method of the dilated cardiomyopathy zebra fish model. The invention constructs the dilated cardiomyopathy zebrafish model of overexpression miR-16-5p in myocardial cells for the first time, and the dilated cardiomyopathy zebrafish model can be used for screening drugs for resisting dilated cardiomyopathy.

The invention discloses a whitening active peptide with an effect of inhibiting melanin formation as well as a preparation method and application of the whitening active peptide. The amino acid sequence of the whitening active peptide is as follows: YYYDPWAGK. Whitening activity verification is carried out on the peptide, the relative tyrosinase activity in cells can be remarkably reduced to 164.26 + / -3.03% from 245.52 + / -1.55% by the peptide with the concentration of 200 mu g / mL, and meanwhile, the relative melanin content can be reduced to 130.05 + / -1.23% from 206.65 + / -4.33%. In addition, the peptide not only can significantly reduce the relative tyrosinase activity of zebrafish from 100 + / -0.36% to 60.42 + / -0.73%, but also can reduce the relative melanin content of zebrafish from 100 + / -2.01% to 63.74 + / -2.05%, and lays an important theoretical foundation for the research and application of zebrafish in the fields of functional food, health care products, cosmetics and medicines.

The invention provides application of active decapeptide in preparation of a medicine for preventing or treating cerebral apoplexy, and belongs to the technical field of biological medicine, the active decapeptide with the amino acid sequence as shown in SEQ ID NO.1 is applied to prevention or treatment of cerebral apoplexy for the first time, the active decapeptide can effectively inhibit formation of cerebral thrombosis of zebra fish with ischemic cerebral apoplexy, and the active decapeptide can be used for preventing or treating cerebral apoplexy. The brain blood flow supply is recovered; meanwhile, the active decapeptide can significantly reduce the hemorrhagic area and hemorrhagic rate of the zebra fish brain with hemorrhagic stroke, repair brain vascular injury and inhibit the occurrence and development of hemorrhagic stroke. In addition, the active decapeptide has small toxic and side effects, and is of great significance for improving the clinical curative effect of cerebral apoplexy and reducing the medication risk when being used for research and development of novel drugs for resisting cerebral apoplexy.

The invention discloses a lactobacillus mucus fermentation strain and application thereof, the lactobacillus mucus fermentation strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on February 21, 2025, and the preservation number is GDMCCNO: 65936. The feruloyl esterase is separated and screened from white sour soup in Kailing in southeast of Guizhou province, has feruloyl esterase activity, and shows good blood fat reducing and fatty liver treating capabilities in a HepG2 high-fat cell model and a high-fat zebra fish model. The compound has a good prospect in the development of products for preventing or treating hyperlipidemia and fatty liver and relieving obesity.

The invention discloses a zebra fish behavior shooting system and method, and relates to the technical field of image processing, and the system comprises a camera which is arranged at the top of a box body; the experiment module is used for culturing zebra fish, and a camera shoots images of the experiment module and the zebra fish; and the computer is used for detecting the edge of the experiment module in the image, taking an area enclosed by the edge as an activity area of the zebra fish, identifying the position of the zebra fish in the activity area, and connecting the positions to form a track diagram of the zebra fish. The application has remarkable advantages in compatibility, and can adapt to various different types of pore plates and self-defined experiment modules. Through flexible hardware configuration and modular design, the system can be customized and adjusted according to experiment requirements, limitation of traditional equipment during experiment switching is avoided, and larger experiment freedom degree and applicability are provided for researchers.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention discloses a siraitia grosvenorii instant coffee beverage assisting in reducing uric acid and a preparation method thereof, and belongs to the technical field of food based on the theory of homology of medicine and food. The coffee beverage is composed of instant coffee powder, a compound sweetening agent, coconut milk powder, milk powder and composite herbal powder in a specific proportion. The compound herbal powder is composed of poria cocos, peach kernels, dried tangerine or orange peel and momordica grosvenori which are selected from a medicinal and edible substance catalog. The herbal active ingredients and a coffee system are deeply fused by optimizing an extraction process, the dual regulation effects of inhibiting xanthine oxidase and promoting uric acid excretion are synergistically exerted, and the technical contradiction that common caffeine possibly affects uric acid excretion is effectively solved. The zebra fish in-vivo experiment proves that the level of uric acid can be remarkably reduced (Plt: 0.001), the improvement rate is as high as 17.63%, and a safe and effective daily diet intervention scheme is provided for people with hyperuricemia while the coffee flavor and convenience are provided.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention discloses a high-flux zebra fish behavior analysis device and method, and aims to solve the problem of compatibility of plate edge distortion and a multi-type pore plate, a telescopic mechanism is arranged in a shell of the device, an isolation cavity is formed in the shell when the telescopic mechanism is retracted, a micro-lens array and a light filter plate are sequentially arranged in the isolation cavity from top to bottom, and the micro-lens array and the light filter plate are arranged in the shell. A stimulation module is arranged at the bottom of the light filter plate, a pore plate placement area is arranged between the stimulation module and the light filter plate, and a universal adaptive bracket for accommodating a standard pore plate and a non-standard pore plate is arranged in the pore plate placement area; a camera module comprising at least one high-frame-rate camera with a fixed focal length is correspondingly arranged above the porous plate placing area, the camera module is connected with a control and analysis module, distortionless track diagrams, heat diagrams and behavior parameters are output online, and the optical mechanical integrated distortion correction and porous plate combined universal adaptation structure is used for correcting the distortion of the porous plate. When the same set of light path is switched between standard plates and non-standard plates of different models, the distortion of the edge view field is automatically compensated.

The invention provides a construction method and application of a dilated cardiomyopathy animal model, belongs to the technical field of biological medicine and animal genetic model construction, and provides a novel dilated cardiomyopathy zebrafish model which is constructed by specifically overexpressing a truncated dnajb6b gene long isomer fragment in zebrafish myocardial cells. The nucleotide sequence of the gene segment is as shown in SEQ ID NO. 1; the invention also provides a construction method and application of the dilated cardiomyopathy zebra fish model, and further discovers application of 4-phenylbutyric acid in preparation of drugs for treating dilated cardiomyopathy.

The invention provides a control method, device and equipment for preparation of hypotonic nutritious food and a storage medium, and relates to the technical field of preparation control of nutritious products. The method comprises the following steps: obtaining a measured osmotic pressure corresponding to a mixed material of the to-be-prepared hypotonic nutritious food; the measured osmotic pressure is obtained by measuring the osmotic pressure of a material sample of the mixed material based on a freezing point method; inputting the measured osmotic pressure into the trained linear regression analysis model to obtain a predicted value of the peristalsis promotion rate; the model is obtained based on milk powder reconstituted fluid osmotic pressure and zebra fish intestine peristalsis promotion rate training. Determining a preparation process optimization scheme according to the predicted value of the peristalsis promotion rate and the early warning condition of the peristalsis promotion rate, and adjusting process parameters according to the preparation process optimization scheme; wherein the process parameters in the optimization scheme of the preparation process comprise one or more of the content range of different types of proteins, the ratio of exogenous calcium to milk-derived calcium, the homogenizing pressure and the spray drying temperature. According to the method, functional evaluation can be rapidly completed, and preparation parameters can be optimized.

The invention provides a hypertrophic cardiomyopathy zebrafish model and a construction method and application thereof, and belongs to the technical field of biology, the hypertrophic cardiomyopathy zebrafish model is constructed by overexpressing miR-29a in myocardial cells of zebrafish, and the sequence of the miR-29a is shown as SEQ ID NO.1; the invention further provides a construction method of the hypertrophic cardiomyopathy zebra fish model. The hypertrophic cardiomyopathy zebrafish model Tg (cmlc2: miR-29a, EGFP) with overexpression of miR-29a in myocardial cells is constructed for the first time, and the hypertrophic cardiomyopathy zebrafish model Tg (cmlc2: miR-29a, EGFP) can be used for screening drugs for resisting hypertrophic cardiomyopathy.

The invention belongs to the technical field of microorganisms, and particularly relates to transgenic chlamydomonas capable of secreting antibacterial peptide outside cells as well as a construction method and application of the transgenic chlamydomonas. Specifically, the invention provides transgenic chlamydomonas capable of secreting antibacterial peptide outside cells. Tests prove that not only can the water quality be improved and the immunity of fishes be improved, but also the step of purifying the antibacterial peptide protein can be effectively omitted. The zebra fish experiment is carried out, and it is proved that the survival rate of the zebra fish can be increased. Meanwhile, compared with an escherichia coli and yeast expression system, the chlamydomonas reinhardtii can also be used as bait of aquatic organisms such as fishes and shrimps, can also be cultured in a closed culture pond, and has important application potential in the aquaculture industry.

The invention discloses a composition for promoting growth and development and application thereof, and relates to the technical field of nutritional compositions. The invention provides a composition for promoting growth and development, the composition is composed of milk mineral salt, colostrum basic protein and hydrolyzed egg yolk powder, and the mass ratio of the milk mineral salt to the colostrum basic protein to the hydrolyzed egg yolk powder is (14-22): (5-10): 1. The composition provided by the invention can promote growth and development, can increase the fluorescence intensity of the skull of the zebra fish and increase the body length of the zebra fish, and has an obvious effect on promoting skeletal development and body development.

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.

The invention discloses application of Trolox in preparation of a medicine for treating toxicity caused by valproic acid, and belongs to the technical field of biological medicine. The invention discovers for the first time that Trolox has a new medicine application of preventing and / or treating developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid; furthermore, animal experiments prove that Trolox can relieve zebra fish development malformation caused by valproic acid and also can relieve zebra fish redox level imbalance caused by valproic acid; this provides a new solution for the prevention and / or treatment of developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid.

The utility model belongs to the technical field of micro-fluidic chips, and particularly relates to a full-automatic zebra fish micro-fluidic chip loading system. Comprising a zebra fish container, a controllable micro-flow pipeline, a zebra fish micro-fluidic chip, a plurality of cameras and an upper computer, the zebra fish container is communicated with the zebra fish micro-fluidic chip through the controllable micro-flow pipeline, and the controllable micro-flow pipeline is further electrically connected with the upper computer; the plurality of cameras are respectively arranged in the controllable micro-flow pipeline and at the top of the zebra fish micro-fluidic chip, and the plurality of cameras are wirelessly connected with the upper computer; the utility model provides a full-automatic zebra fish micro-fluidic chip loading system in order to solve the problems that the existing zebra fish micro-fluidic chip loading process is tedious and time-consuming and the loading flux is generally low, and the full-automatic zebra fish micro-fluidic chip loading system is provided to solve the problems that the zebra fish micro-fluidic chip loading process is tedious and time-consuming in the prior art. The loading efficiency of the zebra fish micro-fluidic chip is improved, and the loading flux is improved on the basis.

The invention relates to an application of extracellular beta-glucan derived from aureobasidium melanogenum in preparation of a training immunity inducer. By establishing a mouse bone marrow derived macrophages (BMDMs) and zebra fish juvenile fish training immune activation evaluation model, the immunocompetence of extracellular beta-glucan (eBG) derived from aureobasidium melanogenum is evaluated, and it is found that the extracellular beta-glucan has a more remarkable training immune activation effect compared with beta-glucan (cBG) derived from cell walls, and the immunocompetence of the extracellular beta-glucan (eBG) derived from aureobasidium melanogenum is improved. It is proved that the extracellular beta-glucan (eBG) from the melanin-producing aureobasidium is an excellent training immunity inducer and has a good application prospect in the fields of feed addition of bred animals and disease immune prevention and control.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.