541 results about "Zebrafish" patented technology

The invention provides a zebra fish multi-target tracking method and system based on a space science experiment video. The method comprises the following steps: firstly, obtaining a zebra fish space science experiment video; then, motion information is extracted based on the zebra fish space science experiment video, and the motion information is used for representing a motion state represented by pixel points in the zebra fish space science experiment video; then, multi-modal feature fusion is carried out based on the motion information and the appearance information, and multi-modal fusion features are determined; and finally, target detection and tracking are carried out according to the multi-modal fusion features, and target movement track information corresponding to the target zebra fishes is determined. According to the method, motion feature information representing dynamic changes is extracted through motion modeling based on a self-adaptive threshold value, then the interaction and fusion relation between appearance and motion features is modeled step by step through a heterogeneous graph, and the robustness of target detection, data association and trajectory prediction is enhanced. Therefore, the stability and the accuracy of multi-target tracking of the zebra fish can be effectively improved.

The invention belongs to the technical field of cosmetic efficacy evaluation, and discloses a sunscreen efficacy evaluation method and application thereof, and the method comprises the following steps: building a blank group; establishing a model group; establishing a sample group; performing constant-temperature incubation on the blank group, the model group and the sample group for a first period of time, recording a swimming track, and calculating swimming distance increment and after-sun burning pain relieving efficiency of each group of zebrafish; performing constant-temperature incubation on the blank group, the model group and the sample group for a second duration, recording the tail fin and dorsal fin area of the zebra fish, and calculating the sunburn prevention efficiency; performing constant-temperature incubation on the blank group, the model group and the sample group for a third period of time, recording the head melanin area of the zebra fish, and calculating the anti-sunburn efficiency; and grading the sunscreen effect of the sample. The method disclosed by the invention has the advantages of low cost, short test period, high reproducibility, high visualization degree, intuitive and understandable test result and the like, and can be used for accurately grading the sunscreen effects of chemical sunscreen agents, water-soluble sunscreen cosmetics and raw materials thereof from the whole animal level.

The invention discloses an eel antibacterial polypeptide Ajaspkidin and application thereof in preparation of an anti-vibrio composition. The amino acid sequence of the eel antibacterial polypeptide Ajaspkidin is shown as SEQ ID NO.01. The invention further discloses an application of the eel antibacterial polypeptide The zebra fish antibacterial peptide has the characteristics of excellent molecular characteristics, broad-spectrum antibacterial activity, efficient bactericidal ability, high thermal stability, high biological safety, anti-infection effect on zebra fish infected by vibrio vulnificus, capability of remarkably improving the survival rate of bred animals and the like, and has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to milk-derived pentapeptide for promoting calcium mineral absorption and application of the milk-derived pentapeptide. The CPP containing the high-activity peptide fragment is obtained by optimizing a process, and the influence of the CPP on mineral absorption is verified by utilizing a zebra fish model. Then, the pentapeptide GPFPI with calcium absorption promoting activity is screened out from the CPP in combination with peptidomics and a molecular docking technology, functional verification is carried out, it is found that the pentapeptide presents a dose-dependent trend on Ca < 2 + > transport, the Ca < 2 + > transport amount is remarkably increased by 23.23% at 10 mu M, and meanwhile, zebra fish experiments show that intervention of the GPFPI effectively reverses accumulated optical density value reduction caused by dexamethasone, and the effect of inhibiting calcium absorption is achieved. Compared with a manufacturing module, the skull accumulative optical density value of a GPFPI (2-20 mu M) processing group is remarkably increased. It is shown that the synthetic peptide GPFPI can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention provides an application of WNT7B in construction of a myopia animal model. Homologous genes wnt7ba and wnt7bb of the WNT7B in the zebra fish are knocked out or knocked down by applying a CRISPR / Cas9 gene editing technology, an animal model of which the eye axis length is remarkably increased, the eyeball movement frequency is remarkably reduced and the light response is reduced is obtained, and the result shows that the WNT7B is related to the high myopia. The model provides a favorable tool for pathogenesis and drug screening of high myopia, and has a good application prospect.

The invention discloses a blood protein precise active peptide capable of tonifying qi and blood, improving microcirculation and promoting vasodilation and a preparation method and application thereof, and belongs to the technical field of blood protein peptide. The blood protein precision active peptide comprises at least one of LGFP, IGFP, FPHFN, LFL, LFI, FESF, ILF, LIF, FLL, FIL, FII or FPHFD. The prepared blood protein precision active peptide has multiple biological activities: an antihypertensive effect is achieved by inhibiting ACE and vasoconstriction substances; vasodilation is promoted by activating a PI3K / AKT / eNOS signal channel; a zebra fish model proves that the traditional Chinese medicine composition has the effects of remarkably improving microcirculation and tonifying qi and blood. The preparation method is low in equipment requirement, simple in process, easy to operate and convenient for industrial large-scale production. The method enriches a bioactive peptide library, and is of great significance in promoting technical progress and industrial upgrading in the field of hemoglobulin peptides.

The invention provides a dilated cardiomyopathy zebrafish model and a construction method and application thereof, and belongs to the technical field of biology.The dilated cardiomyopathy zebrafish model is constructed by overexpressing miR-16-5p in myocardial cells of zebrafish, and the sequence of miR-16-5p is shown as SEQ ID NO.1; the invention further provides a construction method of the dilated cardiomyopathy zebra fish model. The invention constructs the dilated cardiomyopathy zebrafish model of overexpression miR-16-5p in myocardial cells for the first time, and the dilated cardiomyopathy zebrafish model can be used for screening drugs for resisting dilated cardiomyopathy.

The invention discloses a whitening active peptide with an effect of inhibiting melanin formation as well as a preparation method and application of the whitening active peptide. The amino acid sequence of the whitening active peptide is as follows: YYYDPWAGK. Whitening activity verification is carried out on the peptide, the relative tyrosinase activity in cells can be remarkably reduced to 164.26 + / -3.03% from 245.52 + / -1.55% by the peptide with the concentration of 200 mu g / mL, and meanwhile, the relative melanin content can be reduced to 130.05 + / -1.23% from 206.65 + / -4.33%. In addition, the peptide not only can significantly reduce the relative tyrosinase activity of zebrafish from 100 + / -0.36% to 60.42 + / -0.73%, but also can reduce the relative melanin content of zebrafish from 100 + / -2.01% to 63.74 + / -2.05%, and lays an important theoretical foundation for the research and application of zebrafish in the fields of functional food, health care products, cosmetics and medicines.

The invention provides application of active decapeptide in preparation of a medicine for preventing or treating cerebral apoplexy, and belongs to the technical field of biological medicine, the active decapeptide with the amino acid sequence as shown in SEQ ID NO.1 is applied to prevention or treatment of cerebral apoplexy for the first time, the active decapeptide can effectively inhibit formation of cerebral thrombosis of zebra fish with ischemic cerebral apoplexy, and the active decapeptide can be used for preventing or treating cerebral apoplexy. The brain blood flow supply is recovered; meanwhile, the active decapeptide can significantly reduce the hemorrhagic area and hemorrhagic rate of the zebra fish brain with hemorrhagic stroke, repair brain vascular injury and inhibit the occurrence and development of hemorrhagic stroke. In addition, the active decapeptide has small toxic and side effects, and is of great significance for improving the clinical curative effect of cerebral apoplexy and reducing the medication risk when being used for research and development of novel drugs for resisting cerebral apoplexy.

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention discloses a siraitia grosvenorii instant coffee beverage assisting in reducing uric acid and a preparation method thereof, and belongs to the technical field of food based on the theory of homology of medicine and food. The coffee beverage is composed of instant coffee powder, a compound sweetening agent, coconut milk powder, milk powder and composite herbal powder in a specific proportion. The compound herbal powder is composed of poria cocos, peach kernels, dried tangerine or orange peel and momordica grosvenori which are selected from a medicinal and edible substance catalog. The herbal active ingredients and a coffee system are deeply fused by optimizing an extraction process, the dual regulation effects of inhibiting xanthine oxidase and promoting uric acid excretion are synergistically exerted, and the technical contradiction that common caffeine possibly affects uric acid excretion is effectively solved. The zebra fish in-vivo experiment proves that the level of uric acid can be remarkably reduced (Plt: 0.001), the improvement rate is as high as 17.63%, and a safe and effective daily diet intervention scheme is provided for people with hyperuricemia while the coffee flavor and convenience are provided.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention discloses a high-flux zebra fish behavior analysis device and method, and aims to solve the problem of compatibility of plate edge distortion and a multi-type pore plate, a telescopic mechanism is arranged in a shell of the device, an isolation cavity is formed in the shell when the telescopic mechanism is retracted, a micro-lens array and a light filter plate are sequentially arranged in the isolation cavity from top to bottom, and the micro-lens array and the light filter plate are arranged in the shell. A stimulation module is arranged at the bottom of the light filter plate, a pore plate placement area is arranged between the stimulation module and the light filter plate, and a universal adaptive bracket for accommodating a standard pore plate and a non-standard pore plate is arranged in the pore plate placement area; a camera module comprising at least one high-frame-rate camera with a fixed focal length is correspondingly arranged above the porous plate placing area, the camera module is connected with a control and analysis module, distortionless track diagrams, heat diagrams and behavior parameters are output online, and the optical mechanical integrated distortion correction and porous plate combined universal adaptation structure is used for correcting the distortion of the porous plate. When the same set of light path is switched between standard plates and non-standard plates of different models, the distortion of the edge view field is automatically compensated.

The invention provides a control method, device and equipment for preparation of hypotonic nutritious food and a storage medium, and relates to the technical field of preparation control of nutritious products. The method comprises the following steps: obtaining a measured osmotic pressure corresponding to a mixed material of the to-be-prepared hypotonic nutritious food; the measured osmotic pressure is obtained by measuring the osmotic pressure of a material sample of the mixed material based on a freezing point method; inputting the measured osmotic pressure into the trained linear regression analysis model to obtain a predicted value of the peristalsis promotion rate; the model is obtained based on milk powder reconstituted fluid osmotic pressure and zebra fish intestine peristalsis promotion rate training. Determining a preparation process optimization scheme according to the predicted value of the peristalsis promotion rate and the early warning condition of the peristalsis promotion rate, and adjusting process parameters according to the preparation process optimization scheme; wherein the process parameters in the optimization scheme of the preparation process comprise one or more of the content range of different types of proteins, the ratio of exogenous calcium to milk-derived calcium, the homogenizing pressure and the spray drying temperature. According to the method, functional evaluation can be rapidly completed, and preparation parameters can be optimized.

The invention provides a hypertrophic cardiomyopathy zebrafish model and a construction method and application thereof, and belongs to the technical field of biology, the hypertrophic cardiomyopathy zebrafish model is constructed by overexpressing miR-29a in myocardial cells of zebrafish, and the sequence of the miR-29a is shown as SEQ ID NO.1; the invention further provides a construction method of the hypertrophic cardiomyopathy zebra fish model. The hypertrophic cardiomyopathy zebrafish model Tg (cmlc2: miR-29a, EGFP) with overexpression of miR-29a in myocardial cells is constructed for the first time, and the hypertrophic cardiomyopathy zebrafish model Tg (cmlc2: miR-29a, EGFP) can be used for screening drugs for resisting hypertrophic cardiomyopathy.

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.

The invention discloses application of Trolox in preparation of a medicine for treating toxicity caused by valproic acid, and belongs to the technical field of biological medicine. The invention discovers for the first time that Trolox has a new medicine application of preventing and / or treating developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid; furthermore, animal experiments prove that Trolox can relieve zebra fish development malformation caused by valproic acid and also can relieve zebra fish redox level imbalance caused by valproic acid; this provides a new solution for the prevention and / or treatment of developmental toxicity caused by valproic acid or redox level imbalance caused by valproic acid.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention provides a composite herbal cereal protein peptide solid beverage for targeted regulation of uric acid metabolism, and belongs to the technical field of common food developed from medicinal and edible raw materials. According to the invention, Chinese yam, mulberry leaf, gordon euryale seed, lily, coix seed, radish seed, root of kudzu vine, Poria cocos, dried orange peel and other raw materials listed in the catalog of substances which are both food and traditional Chinese medicinal materials in the prior art are carefully selected, and are prepared through scientific compatibility and modern technology. The product not only realizes multi-target reduction of uric acid by inhibiting generation of uric acid and promoting excretion of uric acid, but also is safe and free of toxic and side effects due to the fact that all the components conform to national food safety and medical and edible regulations. A zebra fish in-vivo experiment proves that the uric acid level can be remarkably reduced (Plt; 0.001) under the concentration of 500 mu g / mL. The invention provides a daily food solution integrating the traditional dietary therapy concept and modern scientific verification for people with hyperuricemia.

The invention belongs to the technical field of biology, and particularly relates to casein source heptapeptide for promoting calcium absorption and utilization and application of the casein source heptapeptide. The CPP containing the high-activity peptide fragment is obtained through an optimization process, and the influence of the CPP on mineral absorption and conversion is evaluated by utilizing a mineral deficiency rat model; then, heptapeptide with calcium absorption promoting activity is screened out from the CPP in combination with peptiomics and molecular docking technologies, functional verification is carried out, it is found that when the VAPFPEV is 2 mu M, the Ca < 2 + > transport amount is remarkably increased by 25.25%, meanwhile, zebra fish experiments show that intervention of the VAPFPEV effectively reverses reduction of an accumulated optical density value caused by dexamethasone, and compared with a module making set, the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the The skull accumulative optical density value of a VAPFPEV (1-20 [mu] M) processing group is obviously increased. It is shown that VAPFPEV can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention belongs to the technical field of biological fermentation, and particularly relates to a preparation method and application of a high-activity antioxidant product based on hericium erinaceus-hawthorn solid state fermentation. Activating the strain to prepare a seed solution; adding hawthorn into the rice solid culture medium, inoculating hericium erinaceus, performing constant-temperature culture, drying a fermentation product to constant weight at normal temperature and normal pressure after the culture is finished, crushing, and sieving for later use. The antioxidant effect of the fermentation product is comprehensively evaluated and verified through a systematic in-vitro chemical antioxidant experiment (ABTS < + >, DPPH free radical and hydroxyl free radical scavenging) and an in-vivo zebra fish oxidative stress model, and solid data support is provided for application of the fermentation product in healthy products. The resource high-value utilization way of the hawthorn and the hericium erinaceus is widened, and a technical scheme and a product prototype are provided for developing novel food raw materials, health-care products or medicine auxiliary materials with definite antioxidant functions.

The application discloses a kind of nnUNet zebra fish juvenile whole brain vascular system segmentation methods based on autonomous data set training, it is related to high-resolution imaging technology, image processing and medical image segmentation field, the method makes full use of zebra fish live transparency and fluorescent label advantage, obtains high-resolution whole brain three-dimensional vascular image data, and constructs high-quality segmentation truth value database by semi-automatic segmentation and artificial correction, training is carried out using nnU-Net deep learning model, realize the three-dimensional automatic segmentation of zebra fish brain vascular system signal.The application method significantly improves the degree of automation and precision of image segmentation, effectively solves the problems of low efficiency, high artificial dependence and poor repeatability of traditional brain vascular segmentation.The method is suitable for large-scale high-throughput data processing, can provide efficient, standardized image processing scheme for zebra fish brain vascular development mechanism and brain vascular disease model research, and has wide application prospect.

The application discloses a flavonoid compound in eaglewood as well as a preparation method and application of the flavonoid compound, and relates to the technical field of medicines. The application extracts and separates a new compound from natural eaglewood, and the new compound is used in the preparation of anti-osteoporosis drugs. The compound is detected in vitro to obtain an induction effect of the compound on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts, so as to affect the formation of osteoclasts. Meanwhile, the compound can promote the bone development of zebra fish through RT-qPCR detection, which indicates that the compound has anti-osteoporosis activity. The separation method of the compound is simple and easy to operate.

The invention relates to the field of animal model construction, in particular to a construction method and application of an insomnia zebrafish model. According to the method, a model is constructed by adopting a combined induction mode of continuous white light illumination and superposition of blue light irradiation and pentyltetrazole treatment in the period, so that rapid induction of the insomnia phenotype of the zebra fish is realized. According to the method, environment and chemical factor synergistic induction is combined, and the insomnia degree is represented through multi-dimensional indexes. The zebrafish insomnia model is successfully and stably established, and the zebrafish insomnia model can be used for screening medicines or foods for improving insomnia caused by synergistic effects of environment and chemical factors, and has the advantages of short period, comprehensive phenotype and high reproducibility.

The application discloses application of a complex containing Dendrobium officinale leaf extract in preparation of a whitening and freckle-removing preparation, and the complex comprises the Dendrobium officinale leaf extract, a Rhizoma Polygonati extract and beta arbutin. After the components of the complex are mixed in a specific ratio and fully combined and synergized, the ability of inhibiting tyrosinase activity can be significantly improved by 300%, 175% and 87% respectively compared with the Dendrobium officinale leaf, the Rhizoma Polygonati and the beta arbutin, the melanin generation in zebra fish within 72 hours can be effectively inhibited, the melanin content can be obviously reduced, the melanin inhibition rate is increased by 394%, 532%, 24.14% and 37.39% respectively compared with the Dendrobium officinale leaf group, the Rhizoma Polygonati group, the beta arbutin group and the Dendrobium officinale leaf-Rhizoma Polygonati group, the Dendrobium officinale leaf-Rhizoma Polygonati complex can obviously improve the melanin inhibition rate in zebra fish, the combination of the two and the beta arbutin can make the melanin inhibition rate reach 98.70%, and the whitening and freckle-removing effect of skin can be greatly enhanced.

The invention discloses an image processing method and system for zebra fish flowing blood cell recognition. According to the method, aiming at the problems of cell blurring, stretching and overlapping caused by long-time exposure in microscopic imaging, a complete process including image preprocessing, Transform-based target detection model training and self-adaptive post-processing is constructed. The preprocessing improves the image quality by enhancing the cell edge and the contrast ratio; the detection model performs end-to-end localization on cells by using an improved DETR structure; and in post-processing, an optimal threshold value is automatically selected according to different scenes so as to obtain a stable counting result. According to the method, high recognition precision can be achieved under the complex deformation condition, the good experiment condition generalization ability is achieved, and the method can be used for high-throughput zebra fish hemodynamics research and other scenes.

The invention provides sgRNA for knocking out zebra fish pde6a and application of the sgRNA in visual diseases. According to the sgRNA provided by the invention aiming at a specific position, pde6a of the zebra fish can be knocked out or knocked down in a targeted manner by utilizing a CRISPR / Cas9 system. After pde6a is successfully knocked out or knocked down, a zebra fish model with impaired visual functions and normal retina development is obtained, and the model also has morphological changes such as shorter body length, smaller eyes and shorter inter-ocular distance and also shows a trend of increasing the length of an ocular axis. The zebrafish model provides an experimental tool and a model basis for the research of visual function related diseases.

The invention discloses a method for evaluating improvement of skin inflammation and aging based on a zebra fish model and application thereof. The method comprises the following steps: S1, establishing a blank group; s2, establishing a model group; s3, establishing a sample group; s4, performing constant-temperature incubation; s5, observing the zebrafish of the blank group, the model group and the sample group under a body type fluorescence microscope, and recording the anti-inflammatory efficiency and the anti-wrinkle efficiency of the zebrafish; and S6, evaluating the skin inflammation and aging improving effect of the sample according to an anti-inflammation and anti-aging calculation formula. The method has the advantages of low cost, short test period, high reproducibility, high visualization degree, intuitive and understandable test result and the like, and can be used for accurately grading the efficacy of the cosmetic raw materials for improving skin inflammation and aging from the whole animal level.

The invention discloses sturgeon cartilage active peptide capable of promoting growth and improving bone development as well as a preparation method and application of the sturgeon cartilage active peptide, and belongs to the technical field of active peptide. According to the invention, sturgeon cartilage is taken as a raw material, polypeptide with the highest activity of promoting bone growth and development is selected from different enzymolysis combinations through MC3T3-E1 cell tests, and the positive effect of the polypeptide is verified through zebra fish tests; then, two potential sturgeon cartilage active peptide sequences with the function of promoting bone growth and development are screened out through bioinformatics technologies such as mass spectrum identification, molecular docking and the like, and the amino acid sequences of the sturgeon cartilage active peptide fragments are shown as SEQ ID NO.2 and SEQ ID NO.5. The sturgeon cartilage peptide prepared by the method disclosed by the invention has the activity of promoting bone growth and development, can solve the problem of high-value utilization of sturgeons, can also be applied to functional products as a functional factor, and has a good application prospect.

This invention provides a molecular breeding method for fish with blunted and thickened intermuscular spines, belonging to the fields of gene editing and aquatic organism breeding technology. This invention utilizes CRISPR / Cas9 gene editing technology to mutate the bmp8a gene in fish, thereby obtaining fish with blunted and thickened intermuscular spines. The method of this invention is simple and easy to implement. Verification has shown that this invention can obtain a large number of stably heritable mutant zebrafish through gene knockout, and also provides an important reference for the precise design breeding of other farmed fish. It can serve as an efficient technical means to obtain new germplasm of fish with blunted and thickened intermuscular spines, possessing significant scientific research and industrial application value.