232 results about "On cells" patented technology

The invention provides a cell type annotation method and device based on plant single cell transcriptome data and a readable storage medium. Annotation is achieved through multi-level data integration, wherein preliminary annotation is expressed based on cell type marker genes or homologous genes; calculating expression correlation auxiliary annotations of the to-be-analyzed data and the known transcriptome data set; performing function enrichment on the cell cluster differential genes to deduce cell types; carrying out quasi-timing analysis on the heterogeneous cell clusters and annotating subgroups; and finally integrating and generating a comprehensive annotation. The method solves the problem that the prior art depends on artificial experience and is insufficient in basic data set, and is suitable for mode and non-mode plants.

One aspect of the present disclosure pertains to a memory structure. The memory structure includes multiple memory cells, each of the memory cells includes two active regions extending lengthwise along a first direction and four gate structures extending lengthwise along a second direction perpendicular to the first direction. Each of the four gate structures extend across channel regions of the two active regions. The memory structure further includes a pair of gate-cut dielectric features extending lengthwise along the first direction at cell boundaries between each of the memory cells, each of the gate-cut dielectric features contacts the each of the four gate structures in each of the memory cells.

The disclosure relates to a 5G or 6G communication system for supporting a higher data transmission rate. The present disclosure provides methods for supporting emergency service in wireless network (1000) by UE (100). In an embodiment, when a current cell on which the UE (100) is camped. does not support the emergency service. the method includes executing for the current cell or a frequency of the current cell at least one of: barring the current cell or the frequency of the current cell and starting one of a cell barring timer for the barred current cell or a frequency barring timer for the barred frequency of the current cell. Further, the method includes deprioritizing the current cell or the frequency of the current cell with a lowest priority for performing cell selection or cell reselection. Further, the method includes allowing the UE to perform the cell selection or cell reselection on cells other than the current cell or the frequency of the current cell.

The present invention features the use of cavity acoustic transducers (CATs) to apply mechanical stimuli on cells. CATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear on them. The present invention may use such a portable, automated, and high throughput device for cell transfection.

The invention relates to the field of attack monitoring, and discloses a cellular-based network attack monitoring method and device, equipment and a storage medium. The method comprises the following steps: acquiring to-be-monitored flow data in real time; obtaining a to-be-monitored element spore sequence based on the to-be-monitored flow data; acquiring a feature vector corresponding to the to-be-monitored element spore sequence; inputting the feature vector into a preset reconstruction model to obtain a reconstruction feature vector corresponding to the to-be-monitored element spore sequence; the reconstruction model is obtained through unsupervised training; and determining whether an attack behavior exists according to the feature vector and the reconstructed feature vector. Therefore, the method does not need to depend on the comprehensiveness of attack samples, enables the reconstruction model to find unknown threats under the condition of no labeled data, provides reliable guarantee for industrial network security, and improves the monitoring effect of attack behaviors.

The invention relates to a method and system for detecting cells of a first cell type (1) in a biopsy sample in which a plurality of cells of different cell types (2, 3) are present. The invention further relates to a device for carrying out a magnetic field measurement on cells of a biopsy sample that are labeled with magnetic nanoparticles. The method comprises the following method steps: carrying out tissue dissociation of the biopsy sample in order to singulate the cells present in the biopsy sample; supplying magnetic nanoparticles (11) to the singulated cells in order to selectively label the cells of the first cell type (1); removing the excess magnetic nanoparticles (11); introducing the cells into a microchannel (5), the microchannel (5) having a cross section which allows only one labeled cell (1) to pass through at a time; carrying out a magnetic field measurement in the microchannel (5) by means of a quantum magnetic field sensor (6) based on the F-center principle; and sorting out the labeled cells (1) after they have passed through the microchannel (5).

The invention discloses a cfDNA detection standard substance and a preparation method thereof. The preparation method comprises the following steps: carrying out multiplication culture on cells; performing cell apoptosis treatment on the amplified cells; after cell apoptosis treatment is finished, incubating for a period of time, and collecting supernate; adding the supernate into plasma to prepare a plasma standard substance for cfDNA detection; or extracting cfDNA in the supernate, and preparing a DNA standard substance for cfDNA detection; according to the preparation method, the supernatant of a culture cell line is adopted as a source of a cfDNA detection standard substance, rapid death of cells is promoted through a method of nutrient depletion or cytotoxic reagent addition, the content of mononuclear bodies in the supernatant of the culture medium is increased, the concentration of cfDNA is further increased, and the preparation method is easy to operate, high in yield and low in cost.

The utility model discloses a screen assembling jig, which relates to the technical field of screen processing and comprises a workbench, a mobile phone middle frame placing seat is arranged on the workbench, a mobile phone middle frame placing groove is arranged on the mobile phone middle frame placing seat, and an opening of the mobile phone middle frame placing groove is upward. A screen guide plate is arranged on the workbench, the screen guide plate is movably arranged on the mobile phone middle frame placing seat in a covering mode, a screen guide groove corresponding to the mobile phone middle frame placing groove penetrates through the screen guide plate, and the screen guide groove is arranged in an up-down penetrating mode; a pressing assembly is arranged on the workbench, and the pressing end of the pressing assembly moves downwards to be pressed on the screen from the position above the screen guiding plate through the formed screen guiding groove, so that force is applied to the screen downwards; the pressing assembly on the screen assembling jig is used for pressing operation, other external tools are not needed for auxiliary pressing, and assembling is more convenient.

This application proposes a random access method, user equipment, and storage medium based on cell handover. The method includes: responding to receiving a handover command from a source cell, parsing PRACH configuration information about the target cell from the handover command; detecting a synchronization signal and completing cell synchronization based on the synchronization signal; responding to completing cell synchronization, i.e., sending a preamble sequence, specifically sending the preamble sequence for the first time in the current system frame and for the second time in the next system frame; setting an enlarged first RAR listening window based on the PRACH configuration information; and receiving a RAR from the target cell based on the first RAR listening window, and performing corresponding operations according to the random access type upon receiving the RAR. Based on this, this application eliminates the need to wait for successful decoding of the MIB, thus improving the latency of random access during cell handover and increasing access efficiency.

The invention relates to the technical field of pipetting, in particular to a multifunctional pipetting module based on cell culture, which comprises a pipetting module, a combined frame and a culture base, a plurality of mounting grooves are formed in the upper surface of the culture base, movable bases are rotatably connected in the mounting grooves, and a plurality of pipetting heads are arranged at the bottom of the pipetting module. One end of the rotating shaft on the movable base extends out of the culture base, and a first overturning assembly is fixedly arranged at the end, extending out of the culture base, of the rotating shaft of the movable base. According to the invention, the culture dish is mounted on the movable base, and the turnover assembly on the base is driven by the first driving rod on the pipetting module, so that the turnover assembly can drive the movable base to turn over and incline when the pipetting head moves vertically downwards towards the culture dish, and the culture dish is inclined; furthermore, the culture solution in the culture dish can be concentrated at the corner position of the left side of the culture dish, so that the depth of the culture solution is increased, and a transfer pipette can conveniently suck a culture solution sample.

This invention provides a dry ice transport insulated box for cell products, belonging to the technical field of transport boxes. It includes a box body and lid secured by latches. Inside the box, partitions separate storage compartments for cryopreservation bags, cryopreservation tubes, and dry ice. The dry ice storage compartments are located around the cryopreservation bag and cryopreservation tube storage compartments. Two thermometers are fixed to the side of the box body, with their probes extending into the cryopreservation bag and cryopreservation tube storage compartments respectively, allowing real-time monitoring of the box's internal temperature. The cryopreservation bag storage compartment is used to store strip-shaped cryopreservation boxes containing cryopreservation bags, while the cryopreservation tube storage compartment is used to store square cryopreservation boxes containing cryopreservation tubes. This invention isolates the storage compartments for each cell, preventing collisions and impacts on cell quality stability during transport.

Disclosed are a method and an apparatus for semi-persistent scheduling (SPS) transmission or reception based on cell discontinuous transmission (DTX) and / or cell discontinuous reception (DRX) in a wireless communication system. A method according to one embodiment of the present disclosure may comprise the steps of: receiving, by a terminal, one or more first SPS downlink channels from a network during an active period of cell DTX; and receiving, by the terminal, one or more second SPS downlink channels from the network during a non-active period of the cell DTX. A value of one or more transmission parameters on which the one or more first SPS downlink channels are based and a value of the one or more transmission parameters on which the one or more second SPS downlink channels are based may be different.

The present disclosure relates to a solution on cell off indication via DCI. In particular, a group common DCI format is used to indicate an activation / deactivation of the cell off for entering network energy saving mode. DCI in the group common DCI format may include separate bits for a first indication regarding the activation / deactivation of the cell off for entering network energy saving mode and a second indication regarding the activation / deactivation of cell DTX / DRX. Alternatively, the DCI may include common bits for the first and second indications with a flag to indicate which indication. In this way, the cell off for entering network energy saving mode can be indicated more efficiently.

The invention discloses a differential low-noise integrated biosensor array for cell culture detection and a preparation method thereof, and the differential low-noise integrated biosensor array integrates an ISHFET device, an REHFET device and an EXHFET device, so that extracellular action potential signals and local field potential signals can be detected during in-vitro cell culture; therefore, interference of factors such as tissue trauma and immune response on cell activity signal detection is avoided, and good reliability is realized; the ISHFET device and the REHFET device can form a differential sensing unit, so that the effect of reducing the detection error is realized; the EXHFET device not only can detect extracellular action potential signals, but also can independently detect local field potential signals, so that more diversified cell activity signals can be detected, and more data support is provided for in-vitro cell culture research. The method is widely applied to the technical field of semiconductors.

The present application relates to the technical field of biological medicine, and discloses a kind of living cell carrier and its preparation method and use.The method of the present application utilizes active ester compound containing alkynyl and reacts with living cells, and is connected to the surface of living cells, then combines with RAFT reaction chain transfer molecule, and then utilizes in-situ RAFT reaction to polymerize the polymerization monomer containing active group of required function on cell surface, so as to obtain the cell carrier with required function.

Ducting mitigation in an open radio access network (e.g., using a computerized tool), is enabled. For example, a system can comprise a processor and a memory that stores executable instructions that, when executed by the processor, facilitate performance of operations, comprising, based on cell configuration data representative of a cell configuration applicable to a cellular node and based on meteorological data representative of a meteorological measurement applicable to the cellular node, determining, using a ducting prediction model, whether the cellular node is threshold likely to experience ducting, and in response to determining that the cellular node is threshold likely to experience the ducting, determining, using a ducting mitigation model, a ducting mitigation action to be performed that has been determined to mitigate the ducting.

The present invention discloses a multifunctional oscillating culture integrated shaker and method based on cell culture, which relates to the technical field of enzyme biological cells. A split oscillating platform for placing containers is configured inside the body. The oscillating platform is composed of multiple oscillating plates, and clamping members are configured on the upper part of the oscillating plates to clamp the containers. A driving module connected to the oscillating platform is configured inside the body. The driving module includes an oscillating unit and a connecting unit. The connecting unit connects the oscillating unit and the oscillating plates. The connecting unit is configured with two transmission states for adjacent oscillating plates through a differential, and the differential is configured to drive multiple oscillating plates to oscillate at the same speed or at different speeds by the driving module. In this application, the oscillating platform is split into multiple oscillating plates. When oscillating enzyme biological cells, the enzyme biological cells that require different oscillating parameters are placed on different oscillating plates, and multiple enzyme biological cells can be oscillated in the same shaker through oscillating plates with different oscillating frequencies.

Waveguide effect mitigation in an open wireless access network (e.g., using computerized tools) is supported. For example, a system may include a processor and a memory storing executable instructions that, when executed by the processor, facilitate execution of operations including: determining whether or not a cell configuration is appropriate for a cellular node based on cell configuration data representative of a cell configuration appropriate for the cellular node and based on weather data representative of weather measurements appropriate for the cellular node; determining, using the waveguide effect prediction model, whether the cellular node is a threshold at which it is likely to experience a waveguide effect; and in response to determining that the cellular node is likely to experience the waveguide effect, determining, using a waveguide effect mitigation model, a waveguide effect mitigation action to be performed, the waveguide effect mitigation action to be performed having been determined to mitigate the waveguide effect.

A small molecule compound SPAM1 for up-regulating neuropeptide PACAP and receptor PAC1-R thereof, a method for preparing the same and use of the same; the SPAM1 has a structure of formula (I), wherein R=none, H2O or HCl; SPAM1 has small molecular weight, and can efficiently cross biological barriers including a blood-brain barrier, a blood-testis barrier etc; SPAM1 up-regulates the expression of the neurotransmitter / neuromodulator PACAP secreted by hypothalamus-pituitary and the specific receptor thereof, and acts on the glands downstream of the gonadal axis and the adrenal axis, thereby exerting comprehensive regulation effects; and SPAM1 specifically targets the PAC1-R1, and only acts on cells and tissues of nervous and endocrine systems naturally expressing the PAC1-R, thereby producing fewer side effects. Accordingly, SPAM1 is a novel small molecule compound medicament for effectively treating and preventing function decline and disorders of nervous, endocrine and immune systems closely related to neuropeptide PACAP.

Methods, systems, and devices for wireless communication are described that provide for cell selection or reselection at a user equipment (UE) based on cell selection preference criteria of the UE. The UE may receive a set of cell selection criteria that provides a priority order for cell selection based on one or more cell features, cell types, or combinations thereof. The UE, based on one or more signal measurements of available cells and the cell selection criteria, may select one of the available cells for communications. The UE may also maintain a feature cell database in which a number of cells and an associated feature mask may be stored and used to identify cells having features or types associated with the cell selection criteria for use in cell prioritization. Cell selection criteria may be used for cell selection / reselection procedures, mobility procedures, or any combinations thereof.

A method comprises at a user equipment, establishing, with a network element, a carrier aggregation connection comprising at least two non-contiguous intra-band component carriers in a band with fragmented carriers; receiving, from the network element, a configuration for the at least two component carries in the band with fragmented carriers, wherein when a primary cell resides on a first component carrier in the band with fragmented earners, the configuration comprises a first threshold associated with a condition determining whether to take action to prioritize the primary cell; determining whether the primary cell resides on a first component carrier in the band with fragmented carriers; in response to the determination that the primary cell resides on a first component carrier in the band with fragmented earners, determining whether the condition is satisfied based on the first threshold; in response to the determination that the condition is satisfied, determining a secondary cell on a second component carrier to be deactivated; and deactivating the determined secondary cell.

The present application relates to the technical field of photovoltaic detection, more particularly, the present application relates to a kind of silver aluminum paste lap joint area influence on the test method and device of PERC cell open voltage.Silver aluminum paste lap joint area influence on the test method of PERC cell open voltage, comprising: silver paste is printed in the PERC test silicon piece without laser grooving, after drying, silver paste is printed using the same pattern screen printing, then print aluminum paste, after drying again, the printed silicon piece is sintered, then the electrode after sintering is tested using resistance tester, and the resistance tester reading is recorded.The present application test method is simple, pattern is simple, the influence of silver paste aluminum paste lap joint area on cell open voltage can be accurately measured, the accuracy of evaluating back silver silver paste performance data can be guaranteed, and the test efficiency can be improved, the influence of printing factors and test factors is small, data accuracy is high, and the data obtained by testing is convenient to handle, intuitive.

The application discloses a multi-height position correction method and system based on cell electric impedance measurement, and the correction method comprises the following steps: 1) acquiring an electric impedance signal by adopting a double-difference seven-electrode structure, and performing Gaussian filtering processing on the real part of the electric impedance signal; 2) obtaining characteristic points generated under different heights according to a function of finding extreme values, wherein the characteristic points are extreme points; 3) judging whether the characteristic points are'saddle' type waves according to the number of the characteristic points, and calculating a correction factor; and 4) after judging whether the characteristic points are'saddle' type waves, obtaining the correction factor of the waveform under different heights, and performing scaling on the waveform data under different heights according to a standard factor and the correction factor corresponding to the height, so as to obtain a corrected full-wave image. On the basis of the seven-electrode double-difference, the full-wave correction is performed, so that the seven-electrode double-difference is not only limited to low flow channels, but also can be applied to high flow channels, and the flux is improved.

The invention provides a micro-fluidic chip based on a magnetic response membrane and used for delivering substances in cells, the micro-fluidic chip comprises a chip main body 10, a first surface of the chip main body 10 is provided with a micro-channel 11 and a cavity 12, the middle part of the micro-channel 11 is communicated with the cavity 12 through a transition area 13, and a necking opening, close to the end of the micro-channel 11, of the transition area 13 is a jet flow nozzle 14; a magnetic material film layer 30; the chip base 20 is provided with a through hole 21; wherein the first surface of the chip main body 10 is bonded with the first surface of the magnetic material film layer 30 to seal the microchannel 11 and the cavity 12, the chip base 20 is bonded with the second surface of the magnetic material film layer 30, and the magnetic material film layer 30 is excited by an external magnetic field to generate vibration, so that a culture medium in the cavity 12 generates synthetic jet at the jet nozzle 14; and the material acts on cells flowing through the micro-channel 11, so that the cells are perforated, and the delivery of the material in the cells is flexibly and efficiently realized.

The invention relates to the technical field of cell differentiation, in particular to an ovarian granular cell exosome preparation method and system based on cell directional differentiation, and the method comprises the following steps: reprogramming starting material cells to obtain initial induced pluripotent stem cells, carrying out cell identification to obtain induced pluripotent stem cells, and carrying out directional differentiation on the induced pluripotent stem cells to obtain the ovarian granular cell exosome. Obtaining an ovarian granular cell cluster, obtaining a culture supernatant, purifying the culture supernatant to obtain an initial exosome, carrying out quality detection on the initial exosome to obtain a detection result, if the detection result is that the detection does not reach the standard, obtaining an adjusted exosome, taking the adjusted exosome as the initial exosome, and taking the adjusted exosome as the initial exosome. And returning to the step of detecting the quality of the initial exosome until the detection result is that the detection reaches the standard, and confirming the initial exosome as the target exosome when the detection result is that the detection reaches the standard. The problem that the purity of the exosome is low in the preparation process of the ovarian granular cell exosome at present can be solved.

The utility model discloses a cell cloning loop culture dish, which relates to the technical field of cell culture and comprises cell culture plates with uniform specifications. The cell culture plate is characterized by comprising a bottom plate which is arranged on the bottom surface of the cell culture plate; a cell cloning ring is arranged in the cell culture plate; a top cover is arranged at the upper end of the cell culture plate; the bottom plate and the cell cloning ring are fixed through magnetic attraction and have color difference; the cell cloning ring is arranged in the culture groove of the cell culture plate; the cell cloning ring fixing device has the beneficial effects that the original cell cloning ring is more accurately and stably fixed on the cell culture plate, and meanwhile, pancreatin is not leaked to a non-target area to digest cells. It is guaranteed that all cells obtained after digestion and elution are from the same cell clone cluster, and the purpose of accurately selecting monoclonal cell colonies is achieved.

The invention provides a single cell sorting system and sorting method based on laser-induced jet flow, and belongs to the technical field of biomedical engineering and precise instruments, the single cell sorting system comprises a laser-induced module, a jet flow microfluidic sorting chip and a microscopic imaging module; capturing through cells; target identification and jet flow triggering; collecting single cells; non-contact sorting design is achieved, laser only acts on a special coating material, cells are prevented from being directly irradiated, and phototoxicity is reduced; jet flow impact time lt; the cell stress is uniform, the membrane damage risk is reduced, a multi-parameter sorting strategy is adopted: in combination with multi-dimensional information such as morphology and fluorescence, unmarked rare cells or cells in a specific metabolic state can be screened, a lossless sorting effect and a whole-process closed loop are achieved, and the sorted cells can be directly used for culture or downstream single cell sequencing. The method is combined with an in-situ analysis technology, and integration of sorting and analysis is realized.

A communication method and related apparatus, the method in which a first communication apparatus determines a first signal, the first signal being obtained based on a first sequence and / or a second sequence; wherein the first sequence is determined based on cell information, the second sequence is determined based on one row of elements in N rows of elements contained in the first matrix, N sequences corresponding to the N rows of elements are orthogonal, and N is an integer greater than 1; the first communication device transmits the first signal. In other words, the first signal transmitted by the first communication device may be derived based on the first sequence and / or the second sequence. In this way, the first signal sent by the first communication device can obtain the gain brought by the first sequence and / or the second sequence so as to improve the signal transmission performance.

The invention discloses an SOH estimation method and system suitable for a super capacitor module, and the method comprises the steps: defining a single voltage use boundary, and determining the use upper and lower limits of the single voltage; setting a battery cell equalization starting condition, and when the voltage difference of any single voltage in the system exceeds a set value, triggering a BMS equalization function; determining an energy calculation voltage boundary based on a battery cell consistency requirement; introducing a dynamic voltage boundary range; an effective discharge energy interval is screened, and when the single voltage enters the dynamic voltage range for the first time, the BMS starts to record discharge energy; if it is detected that the current direction is reversed or the system stands before the voltage is reduced to the dynamic range, discarding the recorded discharge energy; and calculating the SOH of the system, calculating the discharge energy in the effective interval, and estimating the health state of the system. A foundation is laid for subsequent calculation by accurately defining a single voltage use boundary based on an actual working condition; and setting a battery cell equalization starting condition optimization energy calculation boundary, and eliminating an abnormal voltage influence caused by battery cell imbalance.

The invention relates to a chip for cell sorting by laser-induced forward transfer, relates to the technical field of cell sorting, and solves the technical problems that in the cell sorting process in the prior art, micro-explosion can strongly impact and damage cells and interfere with research and analysis on cells obtained through sorting. The chip comprises a substrate, a sacrificial layer, an explosion cavity membrane, an isolation layer membrane and a cell cavity membrane which are sequentially arranged from bottom to top. When the chip for cell sorting through laser-induced forward transfer is used for LIFT target cell sorting, impact damage to target cells in the LIFT process can be effectively buffered and reduced, and the activity of the target cells obtained through sorting is improved.