296 results about "On cells" patented technology

The invention relates to the field of cell classification, in particular to a cell classification and function evaluation method and system based on nucleolus morphology. The method provided by the invention comprises the following steps: S101, acquiring microscopic image data of a biological sample; s102, acquiring a first region of interest, a second region of interest and a third region of interest; s103, extracting kernel feature data; s104, carrying out first classification on cells in the biological sample; s105, carrying out second classification on the C-type cells in the biological sample; and S106, calculating the number and / or proportion of the A-type cells and the B-type cells in the biological sample so as to evaluate the functional state of the tissues or organs corresponding to the biological sample. The method can accurately and sensitively reflect and evaluate the functional state of the tissue or organ corresponding to the biological sample, and is especially suitable for the biological sample which is lack of a specific marker and has strong heterogeneity.

The invention provides a system for evaluating an ADE effect in vitro and application of the system. The system comprises: (i) and (iii); (ii) and (iii); or (i), (ii) and (iii); in one embodiment, the present invention relates to a cell comprising: (i) an SARS-CoV-2 pseudovirus, (ii) an antibody that targets the receptor binding domain of SARS-CoV-2, and (iii) a cell that overexpresses CD32B; the cells are CHO cells. The system provided by the invention can be used for evaluating the ADE effect of the to-be-detected SARS-CoV-2 or the pseudovirus prepared from the to-be-detected SARS-CoV-2 or the antibody targeting the SARS-CoV-2, and has the advantage of higher sensitivity compared with a system based on cells expressing CD32A. The ADE evaluation system has a wide detection range, and can evaluate the ADE effect caused by the action of the SARS-CoV-2 virus or the pseudovirus prepared from the SARS-CoV-2 virus on CHO-CD32B in the presence of the RBD antibody with a wide concentration range (16 ng / mL-10000 ng / mL).

A cell reselection method, a cell reselection processing method, and related devices are provided. The cell reselection method includes: receiving, by a terminal, a first message from a network side device. The first message includes first configuration information and second configuration information. The first configuration information includes configuration information of a first signal in a service area. The second configuration information includes configuration information of a first signal in a neighboring area. The cell reselection method further includes performing, by the terminal when the terminal is in a low-power state, low-power cell reselection-related measurement on the first signal based on the first message, to obtain measurement results. The Low-power cell reselection means to determine a to-be-camped-on cell after the terminal leaves the low-power state. The cell reselection method also includes performing, by the terminal, low-power cell reselection based on the measurement results when the terminal leaves the low-power state.

The present disclosure relates to a solution on cell off indication via DCI. In particular, a group common DCI format is used to indicate an activation / deactivation of the cell off for entering network energy saving mode. DCI in the group common DCI format may include separate bits for a first indication regarding the activation / deactivation of the cell off for entering network energy saving mode and a second indication regarding the activation / deactivation of cell DTX / DRX. Alternatively, the DCI may include common bits for the first and second indications with a flag to indicate which indication. In this way, the cell off for entering network energy saving mode can be indicated more efficiently.

The invention provides a cell type annotation method and device based on plant single cell transcriptome data and a readable storage medium. Annotation is achieved through multi-level data integration, wherein preliminary annotation is expressed based on cell type marker genes or homologous genes; calculating expression correlation auxiliary annotations of the to-be-analyzed data and the known transcriptome data set; performing function enrichment on the cell cluster differential genes to deduce cell types; carrying out quasi-timing analysis on the heterogeneous cell clusters and annotating subgroups; and finally integrating and generating a comprehensive annotation. The method solves the problem that the prior art depends on artificial experience and is insufficient in basic data set, and is suitable for mode and non-mode plants.

One aspect of the present disclosure pertains to a memory structure. The memory structure includes multiple memory cells, each of the memory cells includes two active regions extending lengthwise along a first direction and four gate structures extending lengthwise along a second direction perpendicular to the first direction. Each of the four gate structures extend across channel regions of the two active regions. The memory structure further includes a pair of gate-cut dielectric features extending lengthwise along the first direction at cell boundaries between each of the memory cells, each of the gate-cut dielectric features contacts the each of the four gate structures in each of the memory cells.

Provided herein are v-shaped (or generally v-shaped) toroidal bioreactors that can be rotated about an axis. The shape of the container, as well as the ability to rotate it, gives the system the additional degrees of freedom needed for handling several operations commonly performed on cells, including growing, expanding, activating, transducing and manipulating cells in a closed system.

The disclosure relates to a 5G or 6G communication system for supporting a higher data transmission rate. The present disclosure provides methods for supporting emergency service in wireless network (1000) by UE (100). In an embodiment, when a current cell on which the UE (100) is camped. does not support the emergency service. the method includes executing for the current cell or a frequency of the current cell at least one of: barring the current cell or the frequency of the current cell and starting one of a cell barring timer for the barred current cell or a frequency barring timer for the barred frequency of the current cell. Further, the method includes deprioritizing the current cell or the frequency of the current cell with a lowest priority for performing cell selection or cell reselection. Further, the method includes allowing the UE to perform the cell selection or cell reselection on cells other than the current cell or the frequency of the current cell.

The invention discloses a method for detecting the assembly change of an ASGPR (asialoglycoprotein receptor) on the surface of a hepatocyte, which comprises the following steps: carrying out specific labeling on the ASGPR on the surface of the hepatocyte through an antibody, and carrying out fluorescence imaging on the ASGPR on the surface of a cell membrane, the purpose of detecting ASGPR assembly change on the liver cell membrane is achieved by statistically analyzing the number of ASGPR protein positioning points on the surface of the cell membrane and aggregation parameters. According to the method, the distribution and aggregation condition of the ASGPR on the cell surface is measured through the nanoscale resolution, the change of the ASGPR in the cell membrane assembly relation is detected, damage to sample cells is small, the time cost of the experimental process is small, protein positioning is accurate, and as the ASGPR is an endocytosis receptor specifically existing on the surface of hepatocellular carcinoma cells, the detection result is accurate, and the detection accuracy is high. Efficient treatment can be achieved through a drug delivery system formed by targeting ASGPR, ASGPR can be better known by observing the assembly difference of ASGPR on the surface of a cell membrane from the nanoscale, and the method has wide application prospects in the field of medical detection and diagnosis.

The present invention features the use of cavity acoustic transducers (CATs) to apply mechanical stimuli on cells. CATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear on them. The present invention may use such a portable, automated, and high throughput device for cell transfection.

The invention relates to the field of attack monitoring, and discloses a cellular-based network attack monitoring method and device, equipment and a storage medium. The method comprises the following steps: acquiring to-be-monitored flow data in real time; obtaining a to-be-monitored element spore sequence based on the to-be-monitored flow data; acquiring a feature vector corresponding to the to-be-monitored element spore sequence; inputting the feature vector into a preset reconstruction model to obtain a reconstruction feature vector corresponding to the to-be-monitored element spore sequence; the reconstruction model is obtained through unsupervised training; and determining whether an attack behavior exists according to the feature vector and the reconstructed feature vector. Therefore, the method does not need to depend on the comprehensiveness of attack samples, enables the reconstruction model to find unknown threats under the condition of no labeled data, provides reliable guarantee for industrial network security, and improves the monitoring effect of attack behaviors.

The utility model relates to a cell culture dish convenient to separate, which comprises a culture dish body, a dish cover is arranged at the top of the culture dish body, two groups of culture tubes are arranged in the culture dish body, a bearing plate is fixedly arranged in the culture dish body, and two groups of groove bodies are arranged in the culture dish body. Two groups of threaded grooves are formed in the bearing plate, and fixing sleeves are fixedly mounted on the outer surfaces of the two groups of culture tubes. According to the cell culture dish convenient to separate, the fixing sleeve is arranged on the surface of the culture tube, the fixing sleeve is installed in the threaded groove in a threaded mode, the threaded installation mode provides a stable fixing mode, it can be ensured that the culture tube is firmly fixed in the culture dish body, and the displacement risk caused by accidental collision or movement is reduced; by arranging the auxiliary assembly, the stability of cell culture can be further improved, and the influence of external factors on cell growth is reduced.

The present invention provides a molybdenum disulfide nanozyme and a preparation method and application thereof, and relates to the technical field of nanomaterials; the preparation method of the molybdenum disulfide nanozyme comprises using a hydrothermal method to evenly mix a molybdenum source, a sulfur source and a structure directing agent, and then performing a hydrothermal reaction to obtain the molybdenum disulfide nanozyme, wherein the molybdenum source is a MoO2(OH)(OOH) solution. The MoS2 nanozyme provided by the technical solution of the present invention has good stability in a wide pH range (3-11), and in particular, it not only has no toxic effect on cells within the experimental dosage range, but also can protect cells, has a good anti-oxidative stress effect, can significantly reduce the ROS level in cells, increase the activity of SOD in cells, reduce the MDA content in cells, and can safely and efficiently remove oxygen free radicals.

The utility model discloses a cell preservation device capable of monitoring in real time, which relates to the technical field of cell culture, and comprises a preservation box body, a detection mechanism and a refrigeration mechanism, a box door is arranged on one side of the preservation box body, the preservation box body is connected with the box door through a hinge, and a plurality of layers of placing plates are arranged in the preservation box body. The detection mechanism is arranged above the placement plate and is used for detecting cell activity, and the refrigeration mechanism is arranged on the outer side of the storage box body and is used for controlling the temperature in the storage box body. The detection mechanism is arranged, so that a user can detect cell activity in real time, subsequent operation on cells can be carried out in time, the cleaning assembly is arranged, soaking and decontamination operation on a probe are facilitated, pollution to the cell culture dish during detection is prevented, and the refrigeration mechanism is arranged, so that when cells are stored, the cell activity can be detected, and the detection efficiency is improved. The interior of the storage box body is always at lower temperature, so that the storage requirement of cells is met.

The invention relates to a method and system for detecting cells of a first cell type (1) in a biopsy sample in which a plurality of cells of different cell types (2, 3) are present. The invention further relates to a device for carrying out a magnetic field measurement on cells of a biopsy sample that are labeled with magnetic nanoparticles. The method comprises the following method steps: carrying out tissue dissociation of the biopsy sample in order to singulate the cells present in the biopsy sample; supplying magnetic nanoparticles (11) to the singulated cells in order to selectively label the cells of the first cell type (1); removing the excess magnetic nanoparticles (11); introducing the cells into a microchannel (5), the microchannel (5) having a cross section which allows only one labeled cell (1) to pass through at a time; carrying out a magnetic field measurement in the microchannel (5) by means of a quantum magnetic field sensor (6) based on the F-center principle; and sorting out the labeled cells (1) after they have passed through the microchannel (5).

The invention discloses a cfDNA detection standard substance and a preparation method thereof. The preparation method comprises the following steps: carrying out multiplication culture on cells; performing cell apoptosis treatment on the amplified cells; after cell apoptosis treatment is finished, incubating for a period of time, and collecting supernate; adding the supernate into plasma to prepare a plasma standard substance for cfDNA detection; or extracting cfDNA in the supernate, and preparing a DNA standard substance for cfDNA detection; according to the preparation method, the supernatant of a culture cell line is adopted as a source of a cfDNA detection standard substance, rapid death of cells is promoted through a method of nutrient depletion or cytotoxic reagent addition, the content of mononuclear bodies in the supernatant of the culture medium is increased, the concentration of cfDNA is further increased, and the preparation method is easy to operate, high in yield and low in cost.

The utility model discloses a screen assembling jig, which relates to the technical field of screen processing and comprises a workbench, a mobile phone middle frame placing seat is arranged on the workbench, a mobile phone middle frame placing groove is arranged on the mobile phone middle frame placing seat, and an opening of the mobile phone middle frame placing groove is upward. A screen guide plate is arranged on the workbench, the screen guide plate is movably arranged on the mobile phone middle frame placing seat in a covering mode, a screen guide groove corresponding to the mobile phone middle frame placing groove penetrates through the screen guide plate, and the screen guide groove is arranged in an up-down penetrating mode; a pressing assembly is arranged on the workbench, and the pressing end of the pressing assembly moves downwards to be pressed on the screen from the position above the screen guiding plate through the formed screen guiding groove, so that force is applied to the screen downwards; the pressing assembly on the screen assembling jig is used for pressing operation, other external tools are not needed for auxiliary pressing, and assembling is more convenient.

This application proposes a random access method, user equipment, and storage medium based on cell handover. The method includes: responding to receiving a handover command from a source cell, parsing PRACH configuration information about the target cell from the handover command; detecting a synchronization signal and completing cell synchronization based on the synchronization signal; responding to completing cell synchronization, i.e., sending a preamble sequence, specifically sending the preamble sequence for the first time in the current system frame and for the second time in the next system frame; setting an enlarged first RAR listening window based on the PRACH configuration information; and receiving a RAR from the target cell based on the first RAR listening window, and performing corresponding operations according to the random access type upon receiving the RAR. Based on this, this application eliminates the need to wait for successful decoding of the MIB, thus improving the latency of random access during cell handover and increasing access efficiency.

The invention relates to the technical field of pipetting, in particular to a multifunctional pipetting module based on cell culture, which comprises a pipetting module, a combined frame and a culture base, a plurality of mounting grooves are formed in the upper surface of the culture base, movable bases are rotatably connected in the mounting grooves, and a plurality of pipetting heads are arranged at the bottom of the pipetting module. One end of the rotating shaft on the movable base extends out of the culture base, and a first overturning assembly is fixedly arranged at the end, extending out of the culture base, of the rotating shaft of the movable base. According to the invention, the culture dish is mounted on the movable base, and the turnover assembly on the base is driven by the first driving rod on the pipetting module, so that the turnover assembly can drive the movable base to turn over and incline when the pipetting head moves vertically downwards towards the culture dish, and the culture dish is inclined; furthermore, the culture solution in the culture dish can be concentrated at the corner position of the left side of the culture dish, so that the depth of the culture solution is increased, and a transfer pipette can conveniently suck a culture solution sample.

This invention provides a dry ice transport insulated box for cell products, belonging to the technical field of transport boxes. It includes a box body and lid secured by latches. Inside the box, partitions separate storage compartments for cryopreservation bags, cryopreservation tubes, and dry ice. The dry ice storage compartments are located around the cryopreservation bag and cryopreservation tube storage compartments. Two thermometers are fixed to the side of the box body, with their probes extending into the cryopreservation bag and cryopreservation tube storage compartments respectively, allowing real-time monitoring of the box's internal temperature. The cryopreservation bag storage compartment is used to store strip-shaped cryopreservation boxes containing cryopreservation bags, while the cryopreservation tube storage compartment is used to store square cryopreservation boxes containing cryopreservation tubes. This invention isolates the storage compartments for each cell, preventing collisions and impacts on cell quality stability during transport.

Disclosed are a method and an apparatus for semi-persistent scheduling (SPS) transmission or reception based on cell discontinuous transmission (DTX) and / or cell discontinuous reception (DRX) in a wireless communication system. A method according to one embodiment of the present disclosure may comprise the steps of: receiving, by a terminal, one or more first SPS downlink channels from a network during an active period of cell DTX; and receiving, by the terminal, one or more second SPS downlink channels from the network during a non-active period of the cell DTX. A value of one or more transmission parameters on which the one or more first SPS downlink channels are based and a value of the one or more transmission parameters on which the one or more second SPS downlink channels are based may be different.

The present disclosure relates to a solution on cell off indication via DCI. In particular, a group common DCI format is used to indicate an activation / deactivation of the cell off for entering network energy saving mode. DCI in the group common DCI format may include separate bits for a first indication regarding the activation / deactivation of the cell off for entering network energy saving mode and a second indication regarding the activation / deactivation of cell DTX / DRX. Alternatively, the DCI may include common bits for the first and second indications with a flag to indicate which indication. In this way, the cell off for entering network energy saving mode can be indicated more efficiently.

The invention discloses a differential low-noise integrated biosensor array for cell culture detection and a preparation method thereof, and the differential low-noise integrated biosensor array integrates an ISHFET device, an REHFET device and an EXHFET device, so that extracellular action potential signals and local field potential signals can be detected during in-vitro cell culture; therefore, interference of factors such as tissue trauma and immune response on cell activity signal detection is avoided, and good reliability is realized; the ISHFET device and the REHFET device can form a differential sensing unit, so that the effect of reducing the detection error is realized; the EXHFET device not only can detect extracellular action potential signals, but also can independently detect local field potential signals, so that more diversified cell activity signals can be detected, and more data support is provided for in-vitro cell culture research. The method is widely applied to the technical field of semiconductors.

The present application relates to the technical field of biological medicine, and discloses a kind of living cell carrier and its preparation method and use.The method of the present application utilizes active ester compound containing alkynyl and reacts with living cells, and is connected to the surface of living cells, then combines with RAFT reaction chain transfer molecule, and then utilizes in-situ RAFT reaction to polymerize the polymerization monomer containing active group of required function on cell surface, so as to obtain the cell carrier with required function.

Ducting mitigation in an open radio access network (e.g., using a computerized tool), is enabled. For example, a system can comprise a processor and a memory that stores executable instructions that, when executed by the processor, facilitate performance of operations, comprising, based on cell configuration data representative of a cell configuration applicable to a cellular node and based on meteorological data representative of a meteorological measurement applicable to the cellular node, determining, using a ducting prediction model, whether the cellular node is threshold likely to experience ducting, and in response to determining that the cellular node is threshold likely to experience the ducting, determining, using a ducting mitigation model, a ducting mitigation action to be performed that has been determined to mitigate the ducting.

The present invention discloses a multifunctional oscillating culture integrated shaker and method based on cell culture, which relates to the technical field of enzyme biological cells. A split oscillating platform for placing containers is configured inside the body. The oscillating platform is composed of multiple oscillating plates, and clamping members are configured on the upper part of the oscillating plates to clamp the containers. A driving module connected to the oscillating platform is configured inside the body. The driving module includes an oscillating unit and a connecting unit. The connecting unit connects the oscillating unit and the oscillating plates. The connecting unit is configured with two transmission states for adjacent oscillating plates through a differential, and the differential is configured to drive multiple oscillating plates to oscillate at the same speed or at different speeds by the driving module. In this application, the oscillating platform is split into multiple oscillating plates. When oscillating enzyme biological cells, the enzyme biological cells that require different oscillating parameters are placed on different oscillating plates, and multiple enzyme biological cells can be oscillated in the same shaker through oscillating plates with different oscillating frequencies.

Waveguide effect mitigation in an open wireless access network (e.g., using computerized tools) is supported. For example, a system may include a processor and a memory storing executable instructions that, when executed by the processor, facilitate execution of operations including: determining whether or not a cell configuration is appropriate for a cellular node based on cell configuration data representative of a cell configuration appropriate for the cellular node and based on weather data representative of weather measurements appropriate for the cellular node; determining, using the waveguide effect prediction model, whether the cellular node is a threshold at which it is likely to experience a waveguide effect; and in response to determining that the cellular node is likely to experience the waveguide effect, determining, using a waveguide effect mitigation model, a waveguide effect mitigation action to be performed, the waveguide effect mitigation action to be performed having been determined to mitigate the waveguide effect.

A small molecule compound SPAM1 for up-regulating neuropeptide PACAP and receptor PAC1-R thereof, a method for preparing the same and use of the same; the SPAM1 has a structure of formula (I), wherein R=none, H2O or HCl; SPAM1 has small molecular weight, and can efficiently cross biological barriers including a blood-brain barrier, a blood-testis barrier etc; SPAM1 up-regulates the expression of the neurotransmitter / neuromodulator PACAP secreted by hypothalamus-pituitary and the specific receptor thereof, and acts on the glands downstream of the gonadal axis and the adrenal axis, thereby exerting comprehensive regulation effects; and SPAM1 specifically targets the PAC1-R1, and only acts on cells and tissues of nervous and endocrine systems naturally expressing the PAC1-R, thereby producing fewer side effects. Accordingly, SPAM1 is a novel small molecule compound medicament for effectively treating and preventing function decline and disorders of nervous, endocrine and immune systems closely related to neuropeptide PACAP.

The invention discloses a culture, photostimulation and signal processing method of a three-dimensional on-chip brain. The three-dimensional on-chip brain is transfected by using adeno-associated viruses which can specifically express light-sensitive channel proteins, so that cells of the three-dimensional on-chip brain can express the light-sensitive channel proteins on cell membranes of the cells, and 470nm blue light emitted by a light source irradiates the three-dimensional on-chip brain expressing the light-sensitive channel proteins; according to the method, light-sensitive channel protein on a cell membrane is opened, ions in a culture medium enter cells, the ion concentration in the environment is transiently changed, the change of the ions can be detected through a multi-electrode array, and the change range is analyzed; the physiological characteristics of the three-dimensional on-chip brain can be obtained, the function network structure of the three-dimensional on-chip brain is analyzed, and the biological intelligent function of the three-dimensional on-chip brain is disclosed.

Methods, systems, and devices for wireless communication are described that provide for cell selection or reselection at a user equipment (UE) based on cell selection preference criteria of the UE. The UE may receive a set of cell selection criteria that provides a priority order for cell selection based on one or more cell features, cell types, or combinations thereof. The UE, based on one or more signal measurements of available cells and the cell selection criteria, may select one of the available cells for communications. The UE may also maintain a feature cell database in which a number of cells and an associated feature mask may be stored and used to identify cells having features or types associated with the cell selection criteria for use in cell prioritization. Cell selection criteria may be used for cell selection / reselection procedures, mobility procedures, or any combinations thereof.