The invention relates to a copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe. The copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe comprises hexagonal boron nitride nanosheets, copper phthalocyanine molecules and two groups of oligonucleotide binding sequences. One end of each of the two groups of oligonucleotide sequences designed by the invention is a base pair for detecting and circularly amplifying microRNA-21, and the other end is an AS1411 sequence. The AS1411 sequence not only can recognize nucleolin highly expressed on the surfaces of tumor cells to play a role in targeted localization, but also can carry copper phthalocyanine molecules with poor water solubility based on a G quadruplex formed underspecific conditions, then, the nucleotide sequence loaded with copper phthalocyanine is adsorbed on the surfaces of the hexagonal boron nitride nanosheets through pi-pi accumulation to enter tumor cells. The invention further discloses application of the copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe in diagnosis and treatment of tumor cells and living bodies. The diagnosis and treatment integrated nanoprobe can perform in-situ Raman diagnosis on tumor cells, is high in sensitivity and specificity, can realize photodynamic therapy at the same time, and has a certain application value in biological medicine and clinical diagnosis and treatment.