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Method of improving directional imaging ability of carbon dots to cell nucleolus

A technology with cell nucleolus and imaging capabilities, applied in chemical instruments and methods, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of short validity period, expensive reagents, easy bleaching of fluorescence, etc., and achieve improved imaging effects and excellent directional imaging effect of effect

Inactive Publication Date: 2018-06-12
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current commercial reagents and technologies for nucleolus-oriented imaging are mainly concentrated in a few manufacturers, and these reagents are expensive, have a short validity period, and the fluorescence is easy to bleach

Method used

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  • Method of improving directional imaging ability of carbon dots to cell nucleolus
  • Method of improving directional imaging ability of carbon dots to cell nucleolus
  • Method of improving directional imaging ability of carbon dots to cell nucleolus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] CD1 preparation:

[0023] Dissolve 1.6g of citric acid in 10mL of water, add 0.21mL of ethylenediamine to it and mix evenly to form a homogeneous solution (the molar ratio of citric acid and ethylenediamine is 1:3). The solution was transferred to a 50mL polytetrafluoroethylene reactor, and placed in an oven at 180 degrees Celsius for 12 hours. After it was cooled to room temperature, the reaction kettle was opened to obtain a black solution.

[0024] The resulting black solution was transferred to a dialysis bag with a molecular weight cut-off of 1000, and the dialysis bag was placed in a 1L beaker, with constant stirring and water changes to remove unreacted small molecules and generated ultra-small nanoparticles.

[0025] When the solution outside the dialysis bag does not deepen further 10 minutes after the water change, and the solution outside the dialysis bag has no obvious fluorescence under the ultraviolet light, it means that the dialysis is basically complet...

Embodiment 2

[0029] CD2 preparation:

[0030] Dissolve 1.6g of citric acid in 10mL of water, add 0.42mL of ethylenediamine to it and mix evenly to form a homogeneous solution (the molar ratio of citric acid and ethylenediamine is 1:2). The solution was transferred to a 50mL polytetrafluoroethylene reactor, and placed in an oven at 180 degrees Celsius for 12 hours. After it was cooled to room temperature, the reaction kettle was opened to obtain a black solution.

[0031] The resulting black solution was transferred to a dialysis bag with a molecular weight cut-off of 1000, and the dialysis bag was placed in a 1L beaker, with constant stirring and water changes to remove unreacted small molecules and generated ultra-small nanoparticles.

[0032] When the solution outside the dialysis bag does not deepen further 10 minutes after the water change, and the solution outside the dialysis bag has no obvious fluorescence under the ultraviolet light, it means that the dialysis is basically complet...

Embodiment 3

[0036] CD3 preparation:

[0037] Dissolve 1.6g of citric acid in 10mL of water, add 0.83mL of ethylenediamine to it and mix evenly to form a homogeneous solution (the molar ratio of citric acid and ethylenediamine is 2:1). The solution was transferred to a 50mL polytetrafluoroethylene reactor, and placed in an oven at 180 degrees Celsius for 12 hours. After it was cooled to room temperature, the reaction kettle was opened to obtain a black solution.

[0038] The resulting black solution was transferred to a dialysis bag with a molecular weight cut-off of 1000, and the dialysis bag was placed in a 1L beaker, with constant stirring and water changes to remove unreacted small molecules and generated ultra-small nanoparticles.

[0039] When the solution outside the dialysis bag does not deepen further 10 minutes after the water change, and the solution outside the dialysis bag has no obvious fluorescence under the ultraviolet light, it means that the dialysis is basically complet...

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Abstract

The invention discloses a method of improving directional imaging ability of carbon dots to cell nucleolus. The method includes: using citric acid and ethylene diamine as carbon sources to prepare carbon dots; adjusting the surfaces of the carbon dots from negative charge to close to neutral so as to improve directional imaging ability of the carbon dots to the cell nucleolus. Common organic saltis used as a carbon source and simply treated to prepare the carbon dots. Effect of the carbon dots on nucleolus imaging is improved by changing charge on the surfaces of the obtained carbon dots, andthe carbon dots with the surfaces in neutral charge have better directional imaging effect on the nucleolus.

Description

technical field [0001] The invention relates to a method for improving the directional imaging ability of carbon dots on cell nucleoli. Background technique [0002] Carbon dots (CDs) are a new type of luminescent nanoparticles that have developed rapidly in the last 10 years. Carbon dots refer to carbon particles with a particle size of 1-10nm and fluorescent properties. There are many kinds of carbon sources, ranging from chemical reagents commonly used in laboratories to flowers and trees in nature, which can be used as carbon sources to synthesize fluorescent carbon dots. In recent years, the research on carbon dots has made great progress in experiments and theory, and has shown broad application prospects in chemical sensing, photoelectric devices, photocatalysis and other fields. At the same time, it has attracted widespread attention due to its low cost, easy preparation, stable luminescence, safety and non-toxicity, especially in the fields of bioimaging, biomarke...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/65
CPCC09K11/65G01N21/6486
Inventor 朱志军唐建国李海东沈文飞王薇姜倩倩
Owner QINGDAO UNIV
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