372 results about "Carbon dot" patented technology

The invention discloses a metal-doped carbon quantum dot as well as preparation and application of the metal-doped carbon quantum dot in a nano antibacterial agent, citric acid, urea and tea polyphenol are taken as precursors, metal salt is added, carbon dot synthesis and metal ion doping are synchronously completed in one step through a hydrothermal method, and the metal-doped carbon quantum dot is prepared; the surfaces of the metal-doped carbon quantum dots are negatively charged, and the metal-doped carbon quantum dots have peroxidase-like activity and catalase-like activity at the same time; the metal ions adopt any one of Fe < 3 + >, Cu < 2 + > and Ce < 3 + >; the metal-doped carbon quantum dots are in a sphere-like shape. The metal-doped carbon quantum dots prepared by the preparation method disclosed by the invention have peroxidase-like activity and catalase-like activity at the same time through precise doping of metal ions, and the two activities have a synergistic effect in an oral periodontitis microenvironment, so that a good antibacterial effect is achieved.

The invention relates to a high-temperature-resistant and high-salt-resistant carbon dot-hydrogel blocking agent and a preparation method and application thereof.The preparation method comprises the following steps that a reaction monomer, an initiator, an organic cross-linking agent and an inorganic cross-linking agent are added into water to be evenly mixed, and a precursor mixed solution is obtained; adding a polysaccharide polymer into the precursor mixed solution, and stirring and dissolving to obtain a hydrogel prepolymer; polymerizing and partially hydrolyzing the hydrogel prepolymer to obtain partially hydrolyzed interpenetrating network hydrogel; the partially hydrolyzed interpenetrating network hydrogel is carbonized at the constant temperature of 50-70 DEG C to form carbon dots, and the carbon dot-hydrogel blocking agent is obtained. The prepared carbon dot-hydrogel has the advantages that synthesis is green, raw materials are easy to obtain, and toxic reagents do not need to be introduced; the nano enhancement effect of the carbon dots and the physical entanglement effect of the polysaccharide polymer enable the obtained hydrogel to have high toughness and compression strength after high salinity and high-temperature aging, and the hydrogel can be used for profile control and water shutoff of high-temperature and high-salinity oil reservoirs so as to improve the recovery rate of crude oil.

The invention relates to an enzyme response antibacterial carbon dot and a preparation method and application thereof.The carbon dot takes a copper-doped carbon dot as a core and is covalently grafted with a hyaluronic acid shell layer through amidation reaction to form a composite nano material Cu-CDs (at) HA, specifically, soluble copper salt and folic acid are subjected to a hydrothermal reaction to obtain the copper-doped carbon dot, carboxyl is activated through EDC / NHS in a buffer system, and the carbon dot is prepared. And performing grafting reaction with hyaluronic acid to obtain a target product. The hyaluronidase-containing antibacterial composition can be specifically degraded and release antibacterial components under the action of hyaluronidase, shows good bacterial responsive bactericidal performance, has the minimum inhibitory concentrations of 8 g / mL and 16 g / mL for staphylococcus aureus and escherichia coli respectively, and has peroxidase-like activity and free radical scavenging capacity. The antibacterial carbon dots can be used for preparing intelligent wound dressings, antibacterial coatings and other biomedical materials, and have the advantages of responsive drug release, efficient antibacterial property and promotion of wound repair.

The invention provides a hybrid bionic nano delivery system and a preparation method thereof. The hybrid bionic nano delivery system is composed of a carbon dot doped nitrogen carbide-based nanoparticle core and a cell membrane group wrapping the nitrogen carbide-based nanoparticle core. The invention constructs a hybrid bionic nano delivery system, which has good biocompatibility and biological metabolism, is beneficial to application in the field of biomedicine, and can realize low-temperature photothermal therapy of hepatic fibrosis. The carbon nitride-based nano bionic system constructed by the invention is mainly synthesized by high-temperature calcination, ultrasonic treatment and hybrid cell membrane wrapping. The preparation method disclosed by the invention is simple, has good repeatability and has a relatively good industrial production prospect.

The invention relates to the technical field of biological denitrification, in particular to a nitrogen-doped carbon dot-based microbial electronic control denitrification system and application thereof. The system comprises electron donor microorganisms, electron acceptor microorganisms and nitrogen-doped carbon dots, the nitrogen-doped carbon dots serve as electron mediators outside cells, an efficient extracellular electron transfer network is constructed, and interspecific electron transfer resistance is reduced; meanwhile, the nitrogen-doped carbon dots can regulate and control intracellular electron transport chains and the energy metabolism process, the improvement of the reducing power and the energy level is promoted, and the metabolism mode optimization is realized. By cooperatively regulating and controlling generation, transmission and utilization of electrons under an extreme electron donor limited oligotrophic condition, the system disclosed by the invention can realize efficient denitrification, avoid nitrite accumulation, remarkably inhibit generation of nitrous oxide (N2O), reduce dependence on an external organic carbon source and an electron source, and improve the denitrification efficiency. And a new technical approach is provided for improving the deep denitrification efficiency under the low-carbon condition.

The invention discloses a preparation method and application of a dual-channel multifunctional multicolor fluorescent probe, and relates to the technical field of fluorescent sensing detection.The dual-channel multifunctional multicolor fluorescent probe is prepared through the following steps that pyromellitic acid and ZrCl4 solids are dispersed in deionized water, Zr-MOF is prepared through a hydrothermal method, pamoic acid and melamine are dispersed in deionized water, and the Zr-MOF is prepared through a hydrothermal method; the preparation method comprises the following steps: preparing green fluorescent carbon dots (CDs) by using a hydrothermal method, dispersing the CDs and Zr-MOF in deionized water according to a certain proportion, reacting, collecting a brown solid, washing, drying, dispersing the solid in ethanol, adjusting the pH to be alkaline, adding Eu (NO3) 3.6 H2O, reacting, filtering, and drying in vacuum to obtain a target probe. The probe can accurately distinguish Cu < 2 + > ions of tetracycline antibiotics (TCs) through two independent fluorescent channels, not only displays completely independent spectral response, but also has the advantages of self-calibration, high sensitivity, interference resistance, field visual detection and the like. The multifunctional fluorescent probe has important significance for improving detection efficiency, reducing cost and energy consumption, optimizing resource utilization and promoting green low-carbon economy.

The invention discloses a flexible self-powered triboelectric sensor, and belongs to the technical field of sensors, the flexible self-powered triboelectric sensor comprises a first silicone rubber layer, a hydrogel layer, a conductive silica gel electrode layer and a second silicone rubber layer which are sequentially arranged from top to bottom, the hydrogel layer is prepared from N-isopropylacrylamide (NIPAM), N, N-dimethylacrylamide (DMAA), N, N-dimethylacrylamide (DMAA), N, N-dimethylacrylamide (DMAA) and N, N-dimethylacrylamide (DMAA); n, N '-methylene bisacrylamide (MBA) and sodium alginate (SA) are copolymerized to form a three-dimensional network, in-situ embedding of biological source orange juice carbon dots is initiated through ultraviolet light, and the first silicone rubber layer, the hydrogel layer, the conductive silica gel electrode layer and the second silicone rubber layer are bonded and fixed on the periphery through silica gel. The invention discloses a green, safe and high-performance flexible triboelectric sensor, which realizes excellent electrical output through a simple process and is combined with an advanced signal processing technology to realize accurate recognition of multiple key physiological signals by a single sensor.

The invention discloses a method for preparing a polyvinyl alcohol flame-retardant film by using walnut green seedcase carbon dots and application of the polyvinyl alcohol flame-retardant film, and belongs to the technical field of composite materials. The preparation method of the flame-retardant film comprises the following steps: mixing a walnut green seedcase carbon dot solution with polyvinyl alcohol, carrying out oil bath heating treatment, cooling to room temperature, carrying out film forming on the obtained walnut green seedcase carbon dot-polyvinyl alcohol mixed solution in a casting manner, and finally, carrying out drying treatment to obtain the target flame-retardant film. According to the method, the walnut green seedcase is used as a raw material to prepare the carbon dots, hydrolytic tannin and quercetin components contained in the walnut green seedcase are fully utilized, resource recycling of waste is achieved, and the production cost of the flame-retardant film is remarkably reduced. The prepared flame-retardant film has the characteristics of portability and certain mechanical strength, is suitable for various scenes with requirements on light flame-retardant materials, has an excellent flame-retardant effect, can effectively delay or even block flame diffusion, and greatly improves the use safety of the materials.

The invention discloses a covalently bridged bicontinuous hard carbon negative electrode material and a preparation method and application thereof, and belongs to the field of electrochemical energy storage materials. According to the method, the limitation of a biomass hard carbon long-range disordered structure is broken through through a template-self-assembly-reconstruction three-section reaction, atomic-scale synergy of electrons and ions is realized in a hard carbon matrix, and in-situ mechanical interlocking and chemical bridging between heteroatom doped carbon dots and a hard carbon matrix graphite domain are enhanced by utilizing the design of a precursor; topological reconstruction of a carbon skeleton is triggered through pickling purification and a high-temperature carbonization process, and accurate fusion of C-C covalent bonds is realized. The graphitized nano domain grown in the process provides a rapid transmission channel for electrons, generated topological defects and heteroatoms provide a rapid transmission channel for adsorption and surface migration of sodium ions, and the prepared material shows excellent reversible capacity, first coulombic efficiency and rate capability.

The invention discloses a carbon dot, hydrogel containing the carbon dot as well as a preparation method and application of the hydrogel, and relates to the technical field of carbon materials. The carbon dot is prepared from the following raw materials: a hydroxyl-containing flavonoid compound and epsilon-polylysine; the carbon dots contain aniline bonds formed by hydroxyl groups of the hydroxyl-containing flavonoid compounds and amino groups of the epsilon-polylysine. The carbon dot shows higher tumor cell killing property and lower normal cell toxicity than the raw material hydroxyl-containing flavonoid compound at the cellular level, can obviously induce immunogenic death of cancer cells and does not induce up-regulation of extracellular matrix collagen, and also has a better effect of regulating a tumor immune microenvironment.

The invention discloses a preparation method of a high-performance anti-ultraviolet water-repellent polyester fiber. The preparation method comprises the following steps: S1, preparing core-shell composite modified particles; s2, preparing a modified finishing liquid; and S3, carrying out dipping treatment on the polyester fiber by adopting the modified finishing liquid, so as to obtain the high-performance anti-ultraviolet water-repellent polyester fiber. The polyester fiber provided by the invention has excellent ultraviolet resistance and water repellency, titanium dioxide and carbon dots are adopted as main components of an anti-ultraviolet agent, and the core-shell composite modified particles with a special structural system are constructed, so that the excellent ultraviolet resistance can be provided, and the polyester fiber has excellent water repellency. The carbon dots are added into the polyester fiber, the adverse effect caused by the photocatalysis of titanium dioxide can be overcome, the water repellency of the polyester fiber can be improved through the carbon dots, the negative effect of the photocatalysis of titanium dioxide is further reduced, and the carbon dots play multiple improvement roles in the polyester fiber.

The invention belongs to the technical field of gas sensors, and discloses a portable ratio fluorescent film sensor for visual detection of oxygen as well as a preparation method and application of the portable ratio fluorescent film sensor. According to the sensor, polymethyl methacrylate is used as a base material, a carbon dot emitting blue light is used as a fluorescent indicator, protoporphyrin emitting red light is used as a fluorescent reference agent, and the two materials are physically blended to form a blue-red dual-emission system; under the excitation of 365nm ultraviolet light, the oxygen concentration affects the yield of active oxygen, the active oxygen quenches the fluorescence of the carbon dots, the fluorescence of protoporphyrin is stable, and the sensor gradually changes from blue violet to red. Meanwhile, the invention discloses an oxygen quantitative / semi-quantitative detection method of the sensor. The preparation process is simple, the cost is low, the detection operation is simple and convenient, the anti-interference performance is strong, the high-sensitivity visual portable detection of the oxygen concentration can be realized, and the sensor is suitable for the fields of food packaging, medical monitoring, industrial safety and the like, and has the potential of large-scale production and commercial application.

This invention relates to a method for preparing a nanozyme detection reagent for carbofuran residues in apples, belonging to the field of pesticide residue and food safety technology. Specific steps: (1) Prepare mesoporous polydopamine powder using polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer, dopamine hydrochloride, etc.; (2) Prepare a copper-manganese bimetallic carbon dot solution using citric acid, copper chloride, manganese chloride, and ethylenediamine; (3) Prepare the nanozyme detection reagent using the mesoporous polydopamine solution and the copper-manganese bimetallic carbon dot solution. Mesoporous polydopamine has a large specific surface area, providing numerous sites for carbon dot loading and effectively adsorbing carbofuran molecules. The copper-manganese bimetallic carbon dots give the nanozyme detection reagent good enzyme-like activity and generate pesticide concentration-dependent colorimetric and photothermal signals. This invention achieves mutual verification of dual-mode output signals, significantly improving detection reliability; and completes detection within 1.5 h.

The application relates to the frontiers of nanomaterials and drug screening fields, and discloses a fluorescent probe material for drug screening and a drug screening method. The fluorescent probe material realizes screening of anticancer drug efficacy of a to-be-detected drug by detecting the concentration of an NAD+ (nicotinamide adenine dinucleotide) active marker of lactate dehydrogenase, the fluorescent probe material comprises an A agent and a B agent, the A agent is a covalent organic framework material loaded with carbon dots with reducibility, and is denoted as COF@CDs, and the B agent is a transition metal hydride; the transition metal hydride can make NAD+ hydrogenated and reduced into NADH in the presence of the COF@CDs; the product after hydrogen loss of the transition metal hydride can be combined with the COF@CDs, and the COF@CDs can emit fluorescence under excitation light, and the fluorescence is quenched.

The invention discloses a fluorescence sensing system based on nitrogen-doped carbon dots and machine learning and application of the fluorescence sensing system in recognition of benzenediol isomers, and belongs to the technical field of analytical chemistry and artificial intelligence. According to the invention, the undoped carbon dots and the nitrogen-doped carbon dots of which the properties change in a gradient manner are utilized to form the probe library, the carbon dots in the probe library can perform collaborative recognition, and weak differences between analytes are accumulated and amplified. Although the response difference of each single probe in a probe library to two similar isomers is very small, the tiny differences can be accumulated and amplified in a high-dimensional response vector due to the gradient change of the properties of the probes; the microscopic chemical property difference, which is difficult to directly measure, between the isomers is converted into the macroscopic high-dimensional data mode difference which can be easily identified by a machine, so that the accurate identification and classification of the structural similar substances and the mixture thereof are realized.

The invention relates to the technical field of wastewater treatment, in particular to an iron-based nano-enzyme coupled photocatalytic system and application thereof in organic wastewater degradation, and the iron-based nano-enzyme coupled photocatalytic system comprises the following components in percentage by weight: 10-20% of iron-based nano-enzyme, 15-25% of a modified photocatalyst, 25-35% of a porous carrier, 3-8% of an electron mediator, 1-3% of a pH regulator and 0.5-2% of a rare earth element dopant; the iron-based nano-enzyme is nitrogen-doped iron-copper-cobalt trimetal nano-enzyme, the molar ratio of Fe to Cu to Co is (6: 1: 1)-(8: 1: 1), the doping amount of N is 3-6at%, the particle size is 20-50nm, and the peroxidase activity is 120-150U / mg; the modified photocatalyst is graphene quantum dot-carbon dot co-modified titanium dioxide, the total loading capacity is 2-5 wt%, and the forbidden bandwidth is 2.8-3.0 eV; the porous carrier is a mesoporous silica-carbon nanotube-montmorillonite composite material. According to the invention, the synergistic degradation effect is excellent, refractory pollutants can be decomposed, and no toxic intermediates exist; the catalyst is suitable for high-salt and high-turbidity wastewater with different acidity and alkalinity, is good in stability, can be recycled, and is less in metal dissolution; the energy consumption is low, the operation is simple and convenient, the treated wastewater reaches the standard and can be recycled, and the environmental and economic benefits are remarkable.

This invention belongs to the field of composite material preparation technology, and provides an antibacterial activated carbon-silver ion composite photocatalyst and its preparation method. First, a nitrogen-doped titanium dioxide composite carbon dot core material is prepared using a hydrothermal method and high-temperature calcination, with titanium source, nitrogen source, and self-made carbon dots as raw materials. The core material is then surface-modified using a mercaptosilane coupling agent, and nano-silver is loaded in situ onto its surface via chemical reduction to obtain a multifunctional nanopowder. Simultaneously, activated carbon undergoes surface oxidation and amination treatment, and is grafted with the active ester polyethylene glycol maleimide, thereby constructing specific reaction sites on the activated carbon surface. Finally, the multifunctional nanopowder is covalently bonded to the activated carbon carrier surface using the Michael addition reaction between maleimide and mercapto groups. The resulting composite powder is then mixed with a dispersion medium and functional additives to obtain the antibacterial activated carbon-silver ion composite photocatalyst.

The invention belongs to the technical field of chrysin detection, and particularly relates to a dual-emission fluorescent molecularly imprinted polymer as well as a preparation method and application thereof. The method comprises the following steps: preparing carbon dots with different emission wavelengths; carrying out amination modification on the carbon dots; and preparing the dual-emission fluorescent molecularly imprinted polymer. The method also comprises the following steps: respectively adding the dual-emission fluorescent molecularly imprinted polymer into chrysin solutions with different known concentrations, carrying out fluorescence quenching reaction, and establishing a standard curve according to the linear relationship between the detected fluorescence intensity and the corresponding concentration of the chrysin solution; adding the dual-emission fluorescent molecularly imprinted polymer into a solution of a to-be-detected product, and carrying out fluorescence quenching reaction to obtain fluorescence intensity; and calculating the concentration of chrysin in the solution to be detected according to the standard curve. The preparation method is used for solving the technical problems that in the prior art, a single-emission fluorescent molecularly imprinted polymer has defects in the aspects of sensitivity, background fluorescence interference resistance in a complex environment and the like in the detection process.

The invention discloses a preparation method and application of a bimetallic nano-enzyme induced MOFs (at) CDs material, and the preparation method at least comprises the following steps: I, dissolving FeCl3, CoCl2 and 2-aminoterephthalic acid in N, N '-dimethylformamide to form a mixed solution I, and carrying out a reaction I to obtain a bimetallic MOFs material; step II, mixing tricarballylic acid and m-phenylenediamine, and carrying out a reaction II to obtain carbon dot powder; step III, dissolving the carbon dot powder obtained in the step II in methanol, and adding rhodamine B dye to obtain a CDs material; and IV, dissolving the bimetallic MOFs material obtained in the step I and the CDs material obtained in the step III in water, stirring, and drying to obtain the MOFs (at) CDs material. The synthesized nano-enzyme is simple to prepare and high in catalytic activity, the carbon dot material is encapsulated into the MOFs material, the fluorescence characteristic of the MOFs material is enhanced, and the carbon dot material is combined with o-phenylenediamine to be used for detecting H2O2.

The invention discloses a production and preparation method of a multi-position-deviation-prevention flexible circuit board and the flexible circuit board. The invention discloses a multi-position-deviation-prevention flexible circuit board production and preparation method, which comprises the following steps of: firstly, arranging a detection system on the outer side of a flexible circuit pattern layer, printing an outer detection frame in a first pattern layer working procedure, printing an inner detection frame in each subsequent working procedure in sequence, and judging the printing precision of each working procedure in real time by comparing the distance between the inner frame and the outer frame; and each printing is based on the first pattern layer, so that error accumulation is effectively avoided. And secondly, according to the positions of the head carbon dots, on the basis that the detection frame is used for controlling printing deviation, the second die cutting positioning structure synchronously printed with the conductive carbon layer is combined, the die cutting positions of the carbon dots are accurately positioned, appearance die cutting is completed through the first die cutting positioning structure, and the position deviation risk of carbon dot printing and die cutting is remarkably reduced. By means of the method, layering precision control over all stacked pattern layers is achieved, and ultrahigh-precision positioning of key areas is guaranteed from multiple dimensions of printing and die cutting.

The invention discloses a double-emission carbon dot hydrogel film as well as a preparation method and application thereof, and relates to the technical field of analysis detection and functional materials. The double-emission carbon dot hydrogel film takes polyvinyl alcohol hydrogel as a carrier, and double-emission carbon dots (O-CDs) synthesized by taking L-glutamic acid and o-phenylenediamine as precursors are uniformly dispersed in the polyvinyl alcohol hydrogel. The O-CDs have double emission peaks of 400 nm and 530 nm under the excitation of 375 nm. The dual-emission carbon dot hydrogel film is applied to portable visual detection of Cu < 2 + > and Ag < + >, and the portable visual detection is dual-mode measurement based on RGB and comprises fluorescence colorimetric measurement in a solution and visual detection of the film assisted by a smart phone. According to the dual-mode strategy, precise instruments are not needed, rapid, reliable and synchronous detection of Cu < 2 + > and Ag < + > can be achieved within 10 min, the limitation of traditional single-mode detection is broken through, and a stable platform is provided for on-site metal ion monitoring.

The application discloses a preparation method of near-infrared light enhanced response Cu, Fe-Lyz / AgNPs nanoscale enzyme. The method uses Cu, Fe doped carbon dots as a reducing agent to prepare silver nanoparticles, and then the silver nanoparticles are modified by lysozyme to prepare Cu, Fe-Lyz / AgNPs nanoscale enzyme with positive charges on the surface. The Cu, Fe-Lyz / AgNPs nanoscale enzyme has dual activities of peroxidase-like and glutathione oxidase-like and photo-thermal performance. Meanwhile, the near-infrared light can significantly enhance the POD-like activity of the nanoscale enzyme. The Cu, Fe-Lyz / AgNPs nanoscale enzyme has good antibacterial effect on escherichia coli and staphylococcus aureus. Meanwhile, based on the enhancement of the penicillin G potassium on the Cu, Fe-Lyz / AgNPs oxidase-like activity, the application establishes a rapid colorimetric detection method for the penicillin G potassium, and when the method is used for detecting the penicillin G potassium in blood plasma and milk powder samples, the detection limit is as low as 0.05 mu g / mL, and the method has high reliability and accuracy.

This invention belongs to the field of carbon nanomaterials technology, specifically disclosing a method for preparing and applying carbon dots from *Pu Di Lan* (a type of medicinal herb) residue. The specific steps are as follows: dried *Pu Di Lan* residue is pulverized using a cell wall breaker to obtain *Pu Di Lan* residue powder; the powder is then reacted using a hydrothermal method; the reaction solution is collected; the reaction solution is centrifuged, and the supernatant is collected; the supernatant is filtered, and the filtrate is collected; the filtrate is dialyzed, and the retaining liquid is collected; the retaining liquid is freeze-dried to obtain the *Pu Di Lan* residue carbon dots. The obtained *Pu Di Lan* residue carbon dots exhibit blue fluorescence under a 365 nm ultraviolet lamp, and their surface is rich in functional groups such as hydroxyl and carboxyl groups, showing good hydrophilicity. The *Pu Di Lan* residue carbon dots promote β-D-glucosidase activity and can be used in food processing; simultaneously, the fluorescence of the *Pu Di Lan* residue carbon dots is highly sensitive to β-D-glucosidase, and β-D-glucosidase activity can be detected by fitting a linear regression equation with a standard solution.

The application discloses a preparation method of carbon dot material based on blue bacteria, and the carbon dot material is prepared by taking blue bacteria photosystem pigment as raw material. The semiconductor fluorescent carbon dot prepared by the method has stable properties, has wide spectrum absorption to sunlight, can convert ultraviolet light, visible light and infrared light into red light, and has good photoelectric performance. The application further discloses a preparation method of a blue bacteria-based semiconductor fluorescent carbon dot-blue bacteria hybrid, and application of the carbon dot material based on blue bacteria in crop production.

The application discloses a kind of photochromic circular polarized luminescent carbon dots long afterglow composite materials and preparation method and application thereof, belong to chiral luminescent functional material technical field;The composite material with photochromic and long afterglow luminescence characteristics carbon dots as luminescent center, and with cellulose nanocrystal through self-assembly to form chiral composite system with cholesteric helical structure.Carbon dots provide fluorescent emission, photochromic and long afterglow luminescence characteristics, while the chiral structure of cellulose nanocrystal can selectively circularly polarized modulation to luminescence, so as to realize the enhancement of circular polarized luminescent signal.The composite material can realize reversible photochromic luminescence behavior under ultraviolet irradiation, and produce long afterglow emission after excitation stops, while showing obvious circular polarized luminescence characteristics.The material has the advantages of stable structure, simple preparation method, high functional integration, etc., and has good application prospect in information anti-counterfeiting, optical encryption, chiral optoelectronic devices and intelligent display fields.

The application relates to a synthesis method of red luminescent carbon dots with a targeting diagnosis and treatment potential of hepatocellular carcinoma, which comprises the following steps: 1) adding 5-fluorouracil and indocyanine green into deionized water, uniformly mixing, and forming a uniform mixed solution; 2) heating the mixed solution obtained in the step 1) at 160-200 DEG C for 5-9 h, and cooling to room temperature; 3) filtering and dialyzing the solution obtained in the step 2), and obtaining a pure 5-FICD solution; and 4) freeze-drying the solution obtained in the step 3), and obtaining the product. The method takes anticancer drug 5-fluorouracil and photosensitizer indocyanine green as precursors, synthesizes a low-toxicity red light carbon dot through a simple hydrothermal method, improves the shortcoming that a chemotherapy drug has a large side effect, and realizes the targeted killing of hepatocellular carcinoma cells.

The invention belongs to the technical field of preparation and rapid detection of composite materials, and particularly relates to a construction method and application of a high-sensitivity fluorescent probe for rapidly analyzing acrylamide in high-temperature processed food. According to the method, acrylic acid-N-succinimide ester (NAS) is grafted on carbon dots (CDs) and grows into a europium-based fluorescent metal organic framework (Eu-MOF) in situ, a ratio type fluorescent probe capable of being used for high-sensitivity detection is constructed, and compared with traditional technologies for detecting acrylamide in high-temperature processed food through liquid chromatography, enzyme-linked immunosorbent assay and the like, the ratio type fluorescent probe has the advantages that the ratio type fluorescent probe can be used for high-sensitivity detection; the method has the advantages of high sensitivity and high detection speed. The constructed ratio-type fluorescent probe can weaken the interference of external factors, has the advantage of strong anti-interference capability, and can improve the reliability of an analysis result; meanwhile, a visualization method is adopted, colorimetry is combined with a smart phone, and the method is easier to obtain by the public, so that the practical applicability is improved, and the application prospect is wide.

The application discloses a method for reducing the content of stone cells in pear fruits; the method is to spray a carbon dot solution to the flowers and fruits of the pear, and the spraying is performed in three stages, the first directional spraying is performed on the flowers in the full bloom period, the fruit surface and the fruit stalk are sprayed on the first day after the flowers, and the fruits and the surrounding leaves are sprayed on the seventh day after the flowers; the application first constructs a complete technical system for reducing the stone cells of the pear by the carbon dots, can significantly reduce the content of the stone cells by 37.5% to 63%, and can guarantee the stable improvement of the fruit quality, is convenient, safe and environment-friendly, fills the application blank of the carbon dots in the field of accurate regulation and control of the pear quality, provides innovative technical support for fine management of the pear flowers and fruits, and is suitable for popularization and application in large-scale pear planting.

The application discloses a kind of positive charge biomass carbon dots and preparation method and application thereof, belong to biomass carbon dot technical field.The application provides a kind of method for preparing nanoscale biomass carbon dots with positive charge using agricultural waste-tomato seedling, and the preparation method is simple and easy to operate, and the equipment requirement is low, material source is extensive, cheap and easy to obtain, no toxic chemical reagent is used, and it is environment-friendly, and the preparation cost is low;A new way for high-value utilization of tomato seedling is provided;The biomass carbon dots prepared are approximately spherical in shape, uniform in size, well dispersed, and have no aggregation phenomenon, with an average diameter of 10 nm and an average potential of +42.03 mV;Good hydrophilicity, strong stability;With blue, green, red and other fluorescent properties.

The invention belongs to the field of preparation of high-antibacterial carbon dots, and discloses a high-antibacterial carbon dot, a preparation process and application of the high-antibacterial carbon dot in vaginal gel. The prepared carbon dots are prepared by adopting physical shearing and ultrasonic synergistic pretreatment, then cooperating with microwave gradient energy crushing and stripping, and then performing centrifugation, dialysis purification and vacuum freeze drying, the damage of oxidation or acid treatment to a graphite structure is avoided in the whole process, and the carbon dots have excellent antibacterial and antiviral performance, can effectively inactivate various pathogenic microorganisms, and have good application prospects. Comprise herpes simplex virus, human papilloma virus HPV16, staphylococcus aureus, candida albicans and the like. The invention also optimizes application conditions, is suitable for developing efficient and safe vagina antibacterial gel products, and provides an innovative solution for preventing and treating gynecological infection.