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Synthesis method of nitrogen-doped fluorescent carbon dots

A technology of fluorescent carbon dots and synthesis methods, which is applied in the field of synthesis of nitrogen-doped fluorescent carbon dots, which can solve the problems of cumbersome preparation methods, difficult separation and purification, and low quantum yield of fluorescent carbon dots, and achieve uniform size and good water solubility , good repeatability

Inactive Publication Date: 2015-04-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the shortcomings of the current preparation method of fluorescent carbon dots, such as cumbersome preparation methods, low quantum yield and difficult separation and purification, the purpose of the present invention is to provide a new method for synthesizing nitrogen-doped fluorescent carbon dots

Method used

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  • Synthesis method of nitrogen-doped fluorescent carbon dots
  • Synthesis method of nitrogen-doped fluorescent carbon dots
  • Synthesis method of nitrogen-doped fluorescent carbon dots

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Embodiment 1

[0021] Weigh 1.3g of L-arginine solid powder in a 50mL round-bottomed flask at room temperature, add 30mL of deionized water and stir until the solid powder is completely dissolved, then add 0.2g of citric acid weighed, and slowly stir the mixed solution. The pH of the mixed solution is about 7.0; the mixed solution is placed in a polytetrafluoroethylene hydrothermal reactor and reacted at 180°C for 3 hours to obtain a brown-yellow solution; the obtained brown-yellow solution is dialyzed with a dialysis bag with a molecular weight cut-off of 1000Da for 48h, the dialysis process The deionized water should be replaced regularly to remove the unreacted citric acid and L-arginine to obtain a fluorescent carbon dot solution with narrow particle distribution.

[0022] Transmission electron microscopy showed that the obtained fluorescent carbon dots had a particle size range of 9-10nm, and quinine sulfate was used as a reference standard (0.1mol / L H 2 SO 4 , 22°C, the quantum yield is 58...

Embodiment 2

[0024] Weigh 1.3g of L-arginine solid powder in a 50mL round-bottomed flask at room temperature, add 30mL of deionized water and stir until the solid powder is completely dissolved, then add 0.2g of citric acid weighed out, slowly stir the mixed solution, and then mix The pH of the solution is about 7.0; the mixed solution is placed in a polytetrafluoroethylene hydrothermal reactor and reacted at 180°C for 6 hours to obtain a brown-yellow solution; the resulting brown-yellow solution is dialyzed with a dialysis bag with a molecular weight cut-off of 1000Da for 48h, during the dialysis process It is necessary to continuously replace the deionized water and remove the unreacted citric acid and L-arginine to obtain a fluorescent carbon dot solution with a narrow particle distribution.

[0025] Transmission electron microscopy shows that the obtained fluorescent carbon dots have a particle size range of 9-10nm, and quinine sulfate is used as a reference standard (0.1mol / L H 2 SO 4 , 2...

Embodiment 3

[0027] Divide human breast cancer cells into 1×10 5 Cultivate at a density of pcs / mL in a special small dish for confocal laser. After culturing for 24 hours, change the medium to a fresh medium containing 0.2mg / mL fluorescent carbon dots, continue culturing for 20 hours, observe and record the carbon dots under a laser confocal microscope For the labeling of the cells, see the results Figure 4 . The picture is a photo of cells labeled with fluorescent carbon dots under a laser confocal microscope. Figure 4 It shows that under the excitation of 405nm ultraviolet light, the cells labeled with fluorescent carbon dots emit blue fluorescence. The experiment showed that the prepared fluorescent carbon dots emit blue fluorescence in the cells and successfully labeled human breast cancer cells.

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Abstract

The invention belongs to the field of nano light-emitting materials, and relates to a synthesis method of nitrogen-doped fluorescent carbon dots. The nitrogen-doped fluorescent carbon dots are synthesized by a hydrothermal synthesis process by one step by using L-arginine as a carbon source and nitrogen source and citric acid as an assistant. The method comprises the following steps: dissolving L-arginine in deionized water, regulating the pH value of the solution with citric acid, putting the mixed solution in a polytetrafluoroethylene hydrothermal reaction kettle, heating to react to obtain a brown yellow fluorescent carbon dot solution, and finally, dialyzing the fluorescent carbon dot solution in a dialysis bag to remove unreacted raw materials, thereby obtaining the fluorescent carbon dot solution with narrow particle size distribution. The synthesis method is simple to operate, does not need to add any strong acid, strong alkali or any other catalyst, and has the advantages of low raw material cost, no radiation action, environment friendliness and the like; and the obtained fluorescent carbon dots have the advantages of high fluorescent quantum yield and no cytotoxicity, and are successfully applied to cell fluorescence labeling.

Description

Technical field [0001] The invention belongs to the field of nano luminescent materials, and specifically relates to a method for synthesizing nitrogen-doped fluorescent carbon dots. Background technique [0002] Fluorescent carbon dots, as a new type of luminescent carbon nanomaterial, have a particle size generally smaller than or close to the Bohr radius of the exciton. Compared with semiconductor quantum dots and traditional organic dyes, it has the advantages of good water solubility, good biocompatibility, high fluorescence intensity and good stability, resistance to photobleaching and easy biomarking. It is an ideal new fluorescent labeling material. It has broad development prospects in the fields of cell imaging, biomarkers, cancer diagnosis and optoelectronic devices. [0003] At present, the preparation methods of fluorescent carbon dots can be mainly divided into two types: "top-down" and "bottom-up". The "top-down" method mainly pulverizes carbon materials or carbon ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02C09K11/65B82Y30/00
Inventor 顾月清王茜黄珊珊张聪颖周秋梅
Owner CHINA PHARM UNIV
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