195 results about "Quinine" patented technology

The invention relates to a pyrro-quinoline quinine (PQQ) sodium salt derivative and a preparation method thereof, belonging to the field of pharmacy. In the preparation method, PQQ is used as a raw material, and an acid-base neutralization reaction with PQQ is carried out in a basic solvent of sodium hydroxide to prepare the PQQ sodium salt derivative represented in the structural formula (I), wherein R1, R2 and R3 can be identical or different and respectively represents H, ammonium ion (NH3), potassium ion, sodium ion, magnesium ion, calcium ion or zinc ion, and at least one of R1, R2 and R3 is sodium ion. The invention has mild reaction conditions, easy product refining and purification, simple preparation steps and high yield more than 80%. The PQQ sodium salt derivative has the functions of inhibiting GSK-3 activity, reducing senile pigment formation in a transgenic mouse brain, reducing tau protein phosphorylation and the like. Thus, the PQQ sodium salt derivative can be medically used for treating senile dementia and other diseases.

The invention discloses a 6-hydroxyl quinine quaternary ammonium salt asymmetric phase transfer catalyst, a preparation method and application of the 6-hydroxyl quinine quaternary ammonium salt asymmetry phase transfer catalyst and belongs to the field of organic synthesis. In a two-phase system of an oxidizing agent, an alkaline solution and an inert solvent, the 6-hydroxyl quinine quaternary ammonium salt asymmetric phase transfer catalyst converts a beta-dicarbonyl compound into a chiral alpha-hydroxyl-beta-dicarbonyl compound; the usage quantity of the chiral 6-hydroxyl quinine quaternary ammonium salt asymmetric phase transfer catalyst is 0.5-50 mol% of the beta-dicarbonyl compound, and under the mild condition that reaction temperature is -15 DEG C-65 DEG C, the yield of the chiral alpha-hydroxyl-beta-dicarbonyl compound is larger than or equal to 90%, and the enantioselectivity is higher than or equal to 70% ee. The method is mild in reaction condition, environmentally friendly, high in reaction efficiency and suitable for large-scale production and preparation.

The invention discloses a novel synthesis method of palonosetron hydrochloride, which comprises that (1) (S)-tetralin formic acid is reacted with thionyl chloride and (S)-3-amido-quinine cyclic amine, to obtain (S, S)-quinuclidine tetralin formamide, (2), (S, S)-quinuclidine tetralin formamide is reacted with reductant and boron trifluoride diethyl etherate, to obtain (S, S)-tetralin methyl quinine cyclic amine, (3), (S, S)-tetralin methyl quinine cyclic amine is reacted with diphosgene to be added and reacted in boron trifluoride diethyl etherate solution, while the product is added and reacted with alcaine and water, to obtain palonosetron hydrochloride. And the synthesis route is represented as above: a: SOCI2, (S)-3-aminoquinuclidine, b: NaBH4, BF3OEt2, c: BF3OEt2, CICO2CCI3.

The invention belongs to the technical field of organic synthesis, and provides a novel method for preparing a chiral alpha-hydroxy-beta-dicarbonyl compound by using quinine C-2' derivative as a catalyst. The quinine C-2' derivative, of which the amount is 0.5-50 mol%, is used as the catalyst to react with a substrate and an oxidizer in an inert reaction solvent to prepare the chiral alpha-hydroxy-beta-dicarbonyl compound, wherein the maximum yield is 99%, and the maximum enantiomeric excess value is 98%. The inert solvent comprises halohydrocarbon, aromatic hydrocarbon, alkane and the like. The oxidizer is common organic peroxide. The ratio of the oxidizer to the beta-dicarbonyl compound is 1-20, and the reaction temperature is -70 to 50 DEG C. The organic peroxide or oxydol can be used as the oxidizer to respectively obtain two alpha-hydroxylation products in different configuration.

The invention discloses a method for producing pyrroloquinoline quinine through microbial fermentation and a fermentation medium used in the method for producing the pyrroloquinoline quinine through the microbial fermentation. An oxygen control fermentation culture method is used for culturing methylotrophic bacteria, a carbon source contained in the fermentation medium is methyl alcohol, and a nitrogen source contained in the fermentation medium is ammonium sulfate, a dissolved oxygen level is controlled by stages to be 30%-50% of saturated dissolved oxygen in the fermentation process, pH is controlled to be 5.5-6.0 through fed-batch, the fed-batch is achieved through a pH-nitrogen source joint control technology, the methylotrophic bacteria are cultured for six days in a 7.5L fermentation tank, and eventually the cell density (OD 600) exceeds 10, yield of PQQ reaches 2g/L, and production intensity of the PQQ is 333.33mg/L/day. The method for producing the pyrroloquinoline quinine through the microbial fermentation is simple in operation, automatic control and continuous fermentation can be achieved easily, and a solid foundation for industrially producing the pyrroloquinoline quinine through the methylotrophic bacteria is laid.

The invention discloses an analysis method for measuring pyrroloquinoline quinine (PQQ) content through ion pair chromatography, and belongs to the technical field of chromatographic analysis. The PQQ produced through synthesis or fermentation is quantitatively analyzed by ion pair high performance liquid chromatography, the obtained regression equation is y=4E+06X+627846, R<2>=0.9995, and is linear in a test range of 2 to 12 mu g/mL, and the minimum detectable amount is 2.0ng (S/N is more than or equal to 3). The method is simple, and possibly generated impurities in the preparation process are effectively separated.

The invention discloses a Hyphomicrobium sp. strain and a preparation method for pyrroloquinoline quinone. The Hyphomicrobium sp. strain 1112-NTG-2318 capable of realizing high yield of pyrroloquinoline quinine is preserved in China General Microbiological Culture Collection Center with an accession number of CGMCC No. 10709. The preparation method for pyrroloquinoline quinine comprises the following steps: fermenting the Hyphomicrobium sp. strain CGMCC No. 10709 in a fermentation medium and acquiring pyrroloquinoline quinine from fermentation broth. The fermentation titer of the preparation method in a tank with a volume of 80 m<3> reaches 1783 mg/L, so the preparation method is favorable for industrial production of pyrroloquinoline quinine.

Provided is a nanostructured polyphase biomimetic catalyst of quinine and a process for preparing same, belonging to the catalytic field of preparing technology of biomimetic catalyst. The method for preparation of the novel catalyst is that mesoporous material with a nanometer-sized bore diameter is employed to be a vector, a functional group provided by the vector is employed to be a first platform and reacts with substance which has active primitives, and further a second reactive platform is provided and is assembled in situ inside a nanostructured space, thereby forming the objective catalyst. The catalyst has the characteristics that active components are assembled via high dispersion in a one-dimensional or three-dimensional straight-through nanometer-sized pore passage, forming a nanostructured chiral molecule reactor, and displaying the high asymmetric inductivity. The value of the catalyst is higher than the homogeneous phase catalytic level, and the catalyst has the high reutilization property and important adaptation value. Further, the invention is easy to be commercially developed.

The invention discloses a method for preparing a high purity dicaffeoylquinic acid compound by high-efficient separation, mainly comprising: using an alcohol-water solution to extract taraxacum fresh plant or dry plant, carrying out the column chromatographic separation after the concentration, preparing a crude extract rich in dicaffeoylquinic acid compound, then isolating and purifying the crude extract by high-speed countercurrent chromatography, and finally adopting a recrystallization process for refining so as to obtain 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid with high purity. The method is applied to the preparation of high purity monomers by taking various natural products or natural product extracts containing one or a plurality of ingredients in the dicaffeoylquinic acid compound and acquired through various approaches as raw materials, and has advantages of simple steps, simple operation, high efficiency, low cost, easy repetition, and large output of separation and preparation. The purity of dicaffeoylquinic acid compound prepared by the method can reach 98 percent.

The invention discloses methylotrophic bacteria and a method for producing pyrroloquinoline quinine (PQQ) by fermentation thereof, and belongs to the technical field of organisms. A new bacterial strain Methylopilasp.YHT-1 is screened and separated from soil; pyrroloquinoline quinine can be produced by the bacterial strain; the bacterial strain is preserved at the China center for type culture collection on January 12, 2014; the preservation number is CCTCC NO: M2014016. The bacterial strain is subjected to aerobic culture for 3-4 days in a culture medium of taking methyl alcohol as a carbon source, so as to produce 50-113mg/L of pyrroloquinoline quinine. The condition that Methylopilasp can excessively generate PQQ and can secrete out of the cell is reported for the first time, and a new strain is provided for preparation of PQQ by a fermentation method.

The invention relates to poultry feed additive which comprises probiotics enzymic preparation, tea polyphenol, apple polyphenol, amino acid, ethyoxyl quinine, vitamin E, vitamin C and traditional Chinese medicine additive. The probiotics enzymic preparation mainly consists of garment bacillus, bacillus subtilis, enterococcus faecium, streptococcus faecium and xylanase; and the traditional Chinese medicine additive comprises ginger, schisandra chinensis, scutellaria baicalensis, 10-20 parts of folium isatidis, salvia miltiorrhiza, angelica sinensis, rhizoma fagopyri dibotryis, radix paeoniae alba, fructus crataegi and folium ginkgo. The poultry feed additive can adjust the balance of intestinal flora, can recover the intestinal function, can prevent diseases, can improve the production performance, can directly kill and restrain bacteria, and can adjust the body immune function; and in addition, the additive can prevent oil in the feed from being acetified in any period, so that the stability of the feed in the storage process is improved; and the additive has better economic and social benefits.

The invention discloses a complex extraction method for separation and purification of pyrroloquinoline quinine in fermentation broth. The method comprises the steps of: (1) fermenting bacteria to obtain a fermentation broth; (2) subjecting the fermentation broth to high-speed centrifugation, subjecting a supernatant liquid to complex extraction in a two-solute extraction system using trioctylamine as a complexing agent and n-hexane as a diluent, to obtain an upper liquid rich in pyrroloquinoline quinine; (3) subjecting the upper liquid rich in pyrroloquinoline quinine to back extraction with ammonia water, concentrating the ammonia water layer under reduced pressure and lyophilizing to obtain a pyrroloquinoline quinine crude product; and (4) dissolving the pyrroloquinoline quinine crude product in ultrapure water, adjusting the pH to 3-4 by HCl, adding ethanol, stirring at 20-25 DEG C for 5-6 h, and standing for 12-24 h to obtain pyrroloquinoline quinine. The above production method has simple condition and quick process, is easy for large-scale industrial production, and has important meaning to promote the industrialization of pyrroloquinoline quinine.

The invention discloses a preparation method of an alpha-hydroxy-beta-dicarbonyl compound using a cinchona alkaloid derivative as a catalyst. In the condition that the cinchona alkaloid derivative is used as the catalyst, a compound is caused to be in contact with oxidants including oxygen, hydrogen peroxide, and alkyl hydroperoxide; the compound and the oxidant in contact are mixed and stirred in an inert solvent for reaction; after the reaction is ended, the alpha-hydroxy-beta-dicarbonyl compound is obtained by separation, wherein the inert solvent comprises halogenated hydrocarbon, aromatic hydrocarbon, alkane and the like; the dosage molar ratio of the peroxide, which is used as the oxidant, to a beta-dicarbonyl compound is 1-30; the dosage of the catalyst III is 0.5-50mol%; the reaction temperature is minus 78 to 50 DEG C. According to the novel preparation method of the alpha-hydroxy-beta-dicarbonyl compound using the cinchona alkaloid derivative as the catalyst disclosed by the invention, cinchona alkaloid quinine and cinchona alkaloid quinindium, which are main alkaloids in the bark of cinchona and congener, are widely applied to organic unsymmetrical catalytic reaction.

The invention discloses the preparation of imidazole type ionic liquid derived by cinchona alkaloid. The preparation is characterized by adopting quinine or cinchonine to modify 9-hydroxyl of the cinchona alkaloid; carrying out a replacement reaction on chloride salt of obtained imidazole type ionic liquid and inorganic metal salt; preparing imidazole type ionic liquid catalysts of different anions. Compared with the prior art, the preparation disclosed by the invention has the advantages that the catalytic activity is high, the catalysts are easily separated and recycled and can be reused again, the preparation is simple, post-processing is easy, the stability of the catalysts is greatly increased, the application range of the catalysts in a micromolecular organic asymmetric reaction is expanded, reaction conditions are optimized, reaction requirements are reduced, the catalytic activity and the enantioselectivity of a chiral imidazole type ionic liquid catalyst in an asymmetric reaction are further increased, and great significance is obtained for researching medicinal chemistry and medical intermediate compounds.

Biosynthesis of quinine compound and its optimizational ferment process. Strain is Ascomycetes Hypomyces(Fr) Tul.sp. Solid medium contains maize flour, wheat bran and bean dregs. Strain is switched in the medium after disinfection. Ferment at 24-25deg.C above 7 days. Fungus containing quinine compound is obtained. Break and dry the fungus, then extract to obtain the product.

The invention discloses a quinine drug-vincristine drug co-carried liposome and a preparation method thereof. The liposome comprises drugs, a phospholipid, a cholesterol compound and an aqueous medium, wherein the mass ratio of the drugs, the phospholipid and the cholesterol compound is 1:(2-100):(1-35), and the drugs comprise a quinine drug and a vincristine drug. With utilization of the synergistic effect of the quinine drug and the vincristine drug, the quinine drug-vincristine drug co-carried liposome has the characteristics of inhibition of multidrug resistance and reduction of medicating toxicity. The preparation method of the co-carried liposome is simple in preparation process, mild in conditions, fewer control parameters, beneficial for reducing the production cost, and easy to industrially produce.

A method of controlling target aquatic microorganism pest populations by exposing the target population to an effective amount of an aquacidal compound. The aquacidal compounds are selected from the group consisting of quinones, anthraquinones, naphthalenediones, quinine, warfarin, coumarins, amphotalide, cyclohexadiene-1,4-dione, phenidione, pirdone, sodium rhodizonate, apirulosin and thymoquinone. The method is particularly effective for treating ballast water of ships or other enclosed volumes of water subject to transport between or among geographic areas to control the relocation of plants, toxic bacteria, and animals contained in the water.

The invention relates to a preparation of a temperature sensitive quinine chiral sensor and application thereof in electrochemical recognition of a tryptophan enantiomer. The invention includes the following steps: preparation of a quinine chiral sensor and electrochemical recognition of a tryptophan enantiomer. The invention has the beneficial effects that the preparation method of the quinine chiral sensor is simple, and uses the chiral environment character of quinine for preparation of a modified electrode with excellent system performance. At different temperatures, the chiral sensor has the opposite identification effect on an L-/D- tryptophan.

The invention discloses a method for synthesizing a chiral spirocyclo-oxindole-benzopyran-ketone-3,4-dihydro-pyran compound. The method comprises the following steps: using an isatin derivative beta, gama-unsaturated alpha-keto ester and 3-hydroxy-4-hydrogen-chromene-4-ketone as reactants, and synthesizing in a solvent under the catalysis of chiral multifunctional chiral quinine thiourea to obtain a product. The method provided by the invention has the advantages of simple and easy obtaining of raw materials, mild reaction conditions, simple and convenient post-treatment, wide range of suitable substrates, high yield and high enantioselectivity, and the synthesized product can be used for synthesizing intermediates of drugs, insecticides and photoelectric materials.

The invention relates to choline M receptor antagonist aclidinium bromide and a preparation method thereof. The chemical structural formula of the choline M receptor antagonist aclidinium bromide is as shown in the specification. The preparation method comprises the following steps: mixing R-quinine-3 alcohol, alkali and an organic solvent, dripping methyl oxalyl chloride, after the methyl oxalyl chloride is completely dripped, heating, performing heating reflux for 1-20 hours, and separating and purifying, thereby obtaining a substance A; adding iodine into a tetrahydrofuran solution of 2-bromothiophene and magnesium powder to initiate reaction for 1-5 hours, thereby preparing a Grignard reagent of 2-bromothiophene, adding the substance A, stirring to react for 10-30 minutes at the room temperature, performing heating reflux for 4-6 hours, separating and purifying, thereby preparing 2,2-dithienyl-2-glycolic acid-R-quinine-3-base ester, and performing quaterisation reaction with 3-phenoxypropyl bromine, thereby obtaining aclidinium bromide. The aclidinium bromide provided by the invention is simple in reaction operation, high in yield, low in price, short in reaction route, small in waste generation and easy in industrialization production.

The invention discloses a method for producing pyrroloquinoline quinone table vinegar through co-culture and fermentation of microorganisms, and belongs to the technical field of table vinegar brewing. A co-culture system is constructed by saccharomyces cerevisiae, hyphomicrobium and acetobacter pasteurianus, and the table vinegar rich in pyrroloquinoline quinone is produced. The table vinegar rich in pyrroloquinoline quinone is prepared by adopting a method of co-culturing saccharomyces cerevisiae, hyphomicrobium and acetobacter pasteurianus microorganisms through the stages of alcoholic fermentation, acetic fermentation, fermentation stopping, product filtering and packaging and the like, and the concentration of pyrroloquinoline quinone is 0.05-0.2 mg/L. The method is novel, the microorganism co-culture method is adopted, nutritional factors are combined with the table vinegar, the nutritional and healthy value of the table vinegar is increased, and feasibility is achieved.

An animal marking restraint device that is positioned over the animal to prevent the animal from marking while in the house. The animal marking restraint device has an outwardly facing surface impregnated with a bitter chemical such as quinine to prevent the animal from chewing and tearing at the device an inwardly facing animal contact surface impregnated with a flea power formulation to prevent flea infestation of the animal.

The invention discloses a method for producing a 2-keto-L-gulonic acid (2-KLG) vitamin C precursor by modifying Escherichia coli and belongs to the field of genetic engineering. In the method, a sorbitol dehydrogenase (SLDH) gene from gluconobacter oxydans, a pyrroloquinoline quinine (PQQ) gene cluster and a sorbose dehydrogenase (SDH)/ sorbosone dehydrogenase gene (SNDH) from common ketogulonigenium vulgare are expressed in Escherichia coli by a genetic engineering technique to culture an Escherichia coli engineering strain for producing 2-KLG by sorbitol. At present, a two-step fermentationprocess adopted for industrial production of vitamin C in China has the problems of complicated process, many influencing factors, difficult accurate control and large raw material and energy consumption for growing three kinds of microbes at the same time. When the production of the 2-KLG by sorbitol fermentation by the E.coli engineering bacteria is adopted, the production process is simplified, raw materials and energy are saved, and the yield of the 2-KLG can reach 87g/L; and thus, the method has a very bright application prospect.