A method and kit for rapid simultaneous detection of multi-component compounds in body fluids and a method for preparing the same

Abstract

This invention discloses a rapid mass spectrometry method and kit for the simultaneous detection of multiple components in body fluids, belonging to the field of biological detection and identification technology. It can rapidly detect trace compounds such as drugs and biomarkers in complex biological samples. This kit mainly utilizes loaded functional materials to achieve extraction and enrichment of target analytes and is equipped with a quick connector for coupling with a detector to achieve rapid detection. Compared with traditional methods, the significant advantages of the method established by this kit are that it does not require complex sample pretreatment, allows for micro-quantitative sampling, requires small sample volumes, facilitates convenient storage of biological samples, is easy to operate, does not require specialized technical personnel, and enables rapid on-site detection when coupled with portable mass spectrometry. This kit has been successfully used for the detection of quinine, procainamide hydrochloride, atenolol, and nortriptyline in complex samples such as blood and serum, and has significant clinical and economic value.

Description

Technical Field

[0001] This invention relates to the field of detection and identification technology for organisms, and more particularly to a rapid mass spectrometry method and kit for simultaneous detection of multiple components in body fluids, as well as the preparation method thereof. Background Technology

[0002] Point-of-care testing (POCT) refers to a testing method that utilizes portable analytical instruments and accompanying reagents to rapidly obtain test results at the sampling site. From nucleic acid testing to antigen self-testing, POCT products have gained widespread public attention. Stimulated by factors such as continuously increasing market demand, the research and development of POCT products is in a period of rapid growth and faces significant challenges in transformation and upgrading, yet it possesses enormous development potential. POCT technology is not only widely used in public health fields such as clinical testing, major epidemic detection, food safety monitoring, drug testing, and alcohol testing, but it can also be used for individual health management.

[0003] In clinical analysis, point-of-care testing (POCT) detects trace compounds such as drugs and biomarkers in complex biological samples like blood and urine, providing valuable diagnostic information for patient disease examination and prognosis, and playing a crucial role in rapid diagnosis and treatment. However, these clinical samples contain a large amount of cell debris, lipids, and inorganic substances, which can cause severe matrix interference during the detection process, affecting the accuracy of the analytical results. Therefore, traditional analytical methods involve pretreatment of clinical samples such as centrifugation, precipitation, and extraction, making the analytical steps cumbersome and time-consuming, and potentially leading to sample contamination and deterioration. Furthermore, the large sample volume required (typically 2–5 mL for a single blood test) may reduce patient compliance. In addition, the storage conditions for transporting clinical samples to specialized instruments are demanding and costly. Finally, the processing of clinical samples is highly geographically limited, requiring specialized laboratory personnel for sample pretreatment and instrument operation, further increasing the cost of testing.

[0004] Therefore, developing a highly sensitive POCT mass spectrometry method and kit that requires small sample sizes, has simple pretreatment steps, low sample storage and transportation costs, and enables rapid on-site detection has significant clinical and economic value. Summary of the Invention

[0005] In view of the above-mentioned deficiencies of the prior art, in a first aspect of the present invention, a method for preparing a rapid mass spectrometry kit for simultaneous detection of multiple components in body fluids with simple processing is provided, comprising the following steps:

[0006] (1) Functional monomers, crosslinking agents, porogens and initiators are mixed and polymerized at a certain temperature, and then purified to obtain functional materials that specifically capture analytes;

[0007] (2) Using the functional material as the adsorbent for trace analytes, combine it with the general components of the mass spectrometry kit to complete the assembly of a mass spectrometry kit for rapid and simultaneous detection of multi-component compounds in body fluids.

[0008] Preferably, the specific operation of step (1) is as follows: the functional monomer, crosslinking agent, pore-forming agent and initiator are mixed, dissolved by ultrasound and degassed, and polymerized at a certain temperature; after the reaction is completed, the unreacted residues are washed away to obtain the functional material that specifically captures the analyte.

[0009] In this invention, the functional material for specifically capturing analytes refers to a functional material that specifically captures analytes based on at least one principle, such as ion exchange, hydrophilic-hydrophobic interaction, chemical and biological affinity, or chemical reaction.

[0010] Preferably, in step (1), the functional monomer includes one of 3-sulfopropyl methacrylate potassium salt (SPA), glycidylmethacrylate (GMA), 4-vinylphenylboronic acid, and polyethyleneimine.

[0011] Further preferred embodiments include: if the target analyte detected by the mass spectrometry kit is alkaline, the selected functional monomer is potassium 3-sulfopropyl methacrylate based on cation exchange; if the target analyte is acidic, the selected functional monomer is polyethyleneimine based on anion exchange; if the target analyte is a biomolecule containing a cis-dihydroxy structure, such as a nucleoside, sugar, or glycoprotein, the selected functional monomer is 4-vinylphenylboronic acid based on boric acid affinity; and if the target analyte is a small peptide, the selected functional monomer is glycidyl methacrylate based on the specific affinity between the biological ligand and its ligand, and the small peptide ligand is directly modified onto the material surface using the ring-opening reaction of glycidyl methacrylate.

[0012] Preferably, in step (1), the crosslinking agent includes one of N,N'-methylenebisacrylamide (MBA) and ethylene glycol dimethacrylate (EDMA).

[0013] Further preferably, if the mass spectrometry kit requires the detection of a hydrophilic target analyte, the crosslinking agent is selected as N,N'-methylenebisacrylamide; if the target analyte requires the detection of a hydrophobic target analyte, the crosslinking agent is selected as ethylene glycol dimethacrylate.

[0014] Preferably, in step (1), the pore-forming agent includes at least one of water, methanol, cyclohexane, toluene, 1,4-butanediol, dodecanol, and n-propanol.

[0015] Preferably, in step (1), the initiator includes one of 2,2'-azobis(2-methylpropionitrile) (AIBN), ammonium persulfate, and benzoyl peroxide.

[0016] Preferably, in step (1), the mass ratio of the polymer formed by the functional monomer and crosslinking agent to the porogen is in the range of 1:1.5 to 1:3.5; the amount of initiator added is 0.8% to 2% of the total mass of the functional monomer, crosslinking agent and porogen.

[0017] Preferably, in step (1), the polymerization reaction temperature is 50-80°C and the reaction time is 10-14h.

[0018] Preferably, in step (1), the polymerization reaction can be carried out in a reaction vessel comprising one of the following: stainless steel tube, glass tube, syringe needle, capillary tube, solid phase extraction column, magnetic nanomaterial, solid phase extraction pipette tip, thin layer plate, filter paper, filter membrane or glass monomer bottle.

[0019] More preferably, the reaction vessel is pretreated before the reaction by filling it with a mixed solution of equal volumes of N,N-dimethylformamide and 3-(isobutenoyloxy)propyltrimethoxysilane and reacting it at 50-80°C for 10-14 hours, so that double bonds are attached to the inner wall of the vessel, which is more conducive to the polymerization reaction in the vessel.

[0020] Preferably, the specific operation of step (2) is as follows: using the functional material as the adsorbent material for trace analytes, combining it with a quick connector, and equipping it with a corresponding disposable syringe, cleaning agent, and sealing bag, to assemble a mass spectrometry kit for rapid and simultaneous detection of multi-component compounds in body fluids.

[0021] In a second aspect of the present invention, a high-precision, portable, and rapid mass spectrometry kit for simultaneous detection of multiple components in body fluids is provided, which is prepared using the method provided in the first aspect of the present invention.

[0022] In a third aspect of the present invention, a rapid and simple mass spectrometry method for simultaneous detection of multiple components in body fluids is provided, employing a mass spectrometry kit according to the second aspect of the present invention, and comprising the following steps:

[0023] S1. A rapid mass spectrometry kit for simultaneous detection of multiple components in body fluids uses adsorption to quantitatively sample biological samples of body fluids to be tested.

[0024] S2. After sampling, the mass spectrometry kit is cleaned with solvent to remove impurities and fix the target analyte.

[0025] S3. Remove the portion of the mass spectrometry kit from which the target analyte is immobilized for subsequent detection. This portion can be used for immediate detection or for storing and transporting samples for detection when needed.

[0026] S4. Elute the target analyte and proceed to mass spectrometry for detection and analysis.

[0027] Traditional analytical methods for detecting multi-component compounds in body fluids involve pretreatment of clinical samples such as centrifugation, precipitation, and extraction. This makes the analytical process cumbersome and time-consuming, and may also lead to sample contamination and deterioration. Furthermore, the large sample volume required (typically 2-5 mL for a single blood test) may reduce patient compliance. In addition, the storage conditions for transporting clinical samples to specialized instruments are demanding and costly. Finally, the processing of clinical samples is geographically limited, making on-site testing difficult and requiring professional laboratory personnel for sample pretreatment and instrument operation, further increasing the cost. Therefore, this invention develops a low-cost mass spectrometry detection kit that is easy to operate (requiring only four simple steps to begin mass spectrometry detection), has a fast analysis speed (results within 5 minutes), requires only 2 μL of sample, and can be used for both on-site detection and sample preservation and transportation. It has high clinical application and practical value.

[0028] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0029] This invention provides a method for preparing a mass spectrometry kit for rapid and simultaneous detection of multiple components in body fluids. The method is simple to process, allows for personalized testing, and can prepare different types of mass spectrometry kits as needed to achieve targeted testing.

[0030] This invention provides a mass spectrometry kit for rapid and simultaneous detection of multiple components in body fluids. It requires small sample volumes, is highly portable, has low requirements for the operating environment, low storage and transportation costs, and has simple pretreatment steps. It has the capability for rapid on-site detection and high accuracy in detecting trace analytes in complex body fluid samples.

[0031] This invention provides a rapid and simultaneous mass spectrometry method for detecting multiple components in body fluids, which is simple to operate, has a convenient procedure, and provides rapid testing. Attached Figure Description

[0032] Figure 1 This is a schematic diagram illustrating the operation of the mass spectrometry kit of the present invention for detecting multi-component compounds in body fluids;

[0033] Figure 2The results show the performance test results of the mass spectrometry kit in Example 1 at different operating stages when detecting quinine;

[0034] Figure 3 The results show the performance of the mass spectrometry kit used in Example 1 at different operational stages when detecting atenolol.

[0035] Figure 4 The performance test results of the mass spectrometry kit in Example 1 at different operational stages when detecting nortriptyline;

[0036] Figure 5 The performance test results of the mass spectrometry kit of Example 1 at different operating stages when detecting procainamide hydrochloride;

[0037] Figure 6 The results of micro-quantitative sampling and detection of different basic drugs in serum using the mass spectrometry kit of Example 1 are shown.

[0038] Figure 7 The detection results of different basic drugs in blood samples were obtained without using the mass spectrometry kit of Example 1 for extraction and enrichment.

[0039] Figure 8 The results of the extraction and enrichment of different basic drugs in blood samples using the mass spectrometry kit of Example 1 are presented. Detailed Implementation

[0040] The present invention is further illustrated below by way of embodiments, but the invention is not limited to the scope of the embodiments described herein. Experimental methods in the following embodiments that do not specify specific conditions were performed according to conventional methods and conditions, or as selected according to the product instructions.

[0041] To verify the practicality of this mass spectrometry kit, the following representative examples were selected for testing. Specific information regarding the experimental materials is as follows:

[0042] Quinine, 98% purity, CAS number 130-95-0, purchased from Shanghai Bid Pharmaceutical Technology Co., Ltd.

[0043] Atenolol, 98% purity, CAS number 29122-68-7, purchased from Xiangjiutong (Hubei) Biotechnology Co., Ltd.;

[0044] Nortriptyline hydrochloride, purity 99.93%, CAS number 894-71-3, purchased from Shanghai Bid Pharmaceutical Technology Co., Ltd.

[0045] Procainamide hydrochloride, 99% purity, CAS number 614-39-1, purchased from Anaiji Chemical;

[0046] N,N-Dimethylformamide, analytical grade, purchased from Sinopharm Chemical Reagent Co., Ltd.

[0047] 3-(isobutenoyloxy)propyltrimethoxysilane, purity 97%, CAS number 2530-85-0, purchased from Shanghai Maclean Biochemical Technology Co., Ltd.

[0048] Potassium 3-sulfopropyl methacrylate, purity 96%, CAS number 31098-21-2, purchased from Shanghai Aladdin Reagent Co., Ltd.

[0049] Ethylene glycol dimethacrylate, 98% purity, CAS number 97-90-5, purchased from Shanghai Maclean Biochemical Technology Co., Ltd.

[0050] 2,2'-Azobisisobutyronitrile, purity 98%, CAS number 78-67-1, purchased from Shanghai Maclean Biochemical Technology Co., Ltd.

[0051] Dodecanol, chemically pure, purchased from Sinopharm Chemical Reagent Co., Ltd.

[0052] Methanol, LC-MS grade, CAS number 67-56-1, purchased from Hubei Futon Science and Technology Co., Ltd.

[0053] Acetic acid, analytical grade, purchased from Sinopharm Chemical Reagent Co., Ltd.

[0054] Ammonia solution, LC-MS grade, CAS number 1336-21-6, purchased from Shanghai Aladdin Reagent Co., Ltd.

[0055] Purified water, purchased from Hangzhou Wahaha Group Co., Ltd.;

[0056] Quartz capillary tube, inner diameter 530 micrometers, outer diameter 760 micrometers, purchased from Yongnian Fiber Optic Factory, Hebei Province.

[0057] Example 1

[0058] Preparation method of a mass spectrometry kit for rapid simultaneous detection of multiple basic compounds in body fluids:

[0059] (1) Take 100mg of potassium 3-sulfopropyl methacrylate, 150mg of ethylene glycol dimethacrylate, 375mg of dodecanol, 375mg of methanol, 40μL of purified water, and 10mg of 2,2'-azobisisobutyronitrile. After ultrasonic dissolution and degassing, pour the mixture into a pretreated quartz capillary and heat it in a water bath at 60℃ for 12h to carry out the polymerization reaction. After the monolithic material is polymerized in situ in the capillary, the unreacted substances are washed away with methanol to obtain the cation exchange capillary monolithic column, which is a functional material for specifically capturing analytes.

[0060] (2) The prepared cation exchange capillary monolithic column is combined with a quick connector and equipped with a corresponding disposable syringe, cleaning agent and sealing bag to assemble a mass spectrometry kit for rapid simultaneous detection of multi-component compounds in body fluids.

[0061] Example 2

[0062] Four basic drugs—quinine, atenolol, nortriptyline, and procainamide hydrochloride—were selected as target analytes and prepared into 100,000 ppm solutions of acetic acid-water (volume ratio) each. -5 The detection performance of the mass spectrometry kit of Example 1 was studied using a mixed working solution of mol / L.

[0063] like Figure 1 As shown, a rapid mass spectrometry method for simultaneous detection of multiple components in body fluids:

[0064] S1. The mixed working solution is loaded into the capillary monolithic column by flow injection at a flow rate of 15 μL / min, so that the positively charged alkaline drug is captured by the SPA groups in the monolithic column through electrostatic adsorption, thus completing the quantitative sampling of the sample to be tested.

[0065] S2. After sampling, connect the mass spectrometry kit to a syringe containing a cleaning solvent, wash with pure methanol to remove non-target analytes, and fix the target analytes.

[0066] S3. Remove the quick connector from the mass spectrometry kit for subsequent detection;

[0067] S4. The alkaline drug was eluted with a 5% ammonia-methanol solution (volume ratio), and then detected by electrospray ionization (ESI) followed by mass spectrometry. After optimizing the collision-induced dissociation (CID) energy of the target analyte, the extracted ion chromatogram (EIC) of the target analyte was qualitatively and quantitatively analyzed by multiple reaction monitoring (MRM) of mass spectrometry.

[0068] The corresponding detection results in this embodiment can be found in [link / reference]. Figures 2-5 .like Figures 2-5As shown, quinine, atenolol, nortriptyline, and procainamide hydrochloride were not detected during sample loading and washing. During elution, distinct chromatographic ion peaks for all four drugs were observed. Furthermore, the four drugs were confirmed by secondary mass spectrometry parent-daughter ion pairing: quinine (m / z 325→307), atenolol (m / z 267→225), nortriptyline (m / z 264→233), and procainamide hydrochloride (m / z 236→163). These results indicate that the mass spectrometry kit of this invention can effectively detect basic drugs.

[0069] Example 3

[0070] Mass spectrometry kit for micro-quantitative sampling and detection of basic drugs in serum:

[0071] This study investigates the application of the mass spectrometry kit of Example 1 of the present invention in rapid micro-quantitative sampling, and its effectiveness in detecting target analytes in complex serum samples.

[0072] Rapid mass spectrometry method for simultaneous detection of multiple components in body fluids:

[0073] S1. Contact one end of the mass spectrometry reagent kit's column tip with the serum spiked sample (a mixture of four basic drugs: quinine, atenolol, nortriptyline, and procainamide hydrochloride, each at a concentration of 10). -5 (mol / L), and after testing, 2μL of serum sample can be quantitatively aspirated in 10s to complete the quantitative serum sampling;

[0074] S2. Press the monolithic column needle tip onto the quick connector, then connect the monolithic column quick connector to a disposable syringe containing 1 / 10,000 acetic acid methanol (volume ratio) cleaning solution. Push the acetic acid methanol solution in the syringe into the monolithic column to provide an acidic environment so that the alkaline drug can be adsorbed onto the monolithic column and remove non-specifically adsorbed impurities.

[0075] S3. Remove the quick connector of the entire column from the mass spectrometry kit for subsequent detection;

[0076] S4. The monolithic column quick connector is switched to a disposable syringe containing 5% ammonia and methanol (volume ratio), connected to the pneumatic atomizing electrospray device, and the predetermined program is started to elute the target analyte into the mass spectrometer at a flow rate of 30 μL / min. Finally, the target analyte is qualitatively and quantitatively analyzed by mass spectrometry multiple reaction monitoring.

[0077] The analysis results of this embodiment can be found in [link / reference]. Figure 6 .like Figure 6As shown, quinine, atenolol, nortriptyline, and procainamide hydrochloride exhibited distinct chromatographic ion peaks during elution, which can be used for the quantification of target analytes. Furthermore, qualitative analysis by secondary mass spectrometry confirmed the detection of these basic drugs. The results indicate that the mass spectrometry kit of this invention can rapidly perform micro-quantitative sampling and effectively detect basic drugs.

[0078] Example 4

[0079] Research on the extraction, enrichment, and detection of basic drugs in blood samples using mass spectrometry kits:

[0080] This study investigates the effectiveness of the mass spectrometry kit of Example 1 of this invention in the extraction and enrichment of low-concentration analytes in complex blood samples. In this example, the preparation of the mixed working solution is similar to that of Example 2, but the concentrations of the four basic drugs—quinine, atenolol, nortriptyline, and procainamide hydrochloride—are all reduced to 10 after mixing. -7 mol / L. The mixed working solution was not enriched by the monolithic column of the mass spectrometry kit, but was detected by dynamic nebulization electrospray mass spectrometry at a flow rate of 15 μL / min. Multiple reaction monitoring (MRM) revealed that, for example... Figure 7 As shown, the target analyte was not detected due to its low concentration.

[0081] Take 2 μL of blood and spike it with a mixture of four alkaline drugs: quinine, atenolol, nortriptyline, and procainamide hydrochloride, each at a concentration of 10. -5 A solution of 1 mol / L acetic acid (v / v) was added to 198 μL of 1 / 10,000 acetic acid solution to test the enrichment and impurity removal capabilities of the mass spectrometry kit. The analytical solution was manually injected into the monolithic column and washed with pure methanol to remove non-target analytes. The basic drug was eluted with 5% ammonia-methanol solution (v / v) and then detected by mass spectrometry. Qualitative and quantitative analysis of the target analyte was performed using multiple reaction monitoring (MRM). Figure 8 As shown, quinine, atenolol, nortriptyline, and procainamide hydrochloride exhibited distinct chromatographic ion peaks during elution, which can be used for the quantification of target analytes. Furthermore, qualitative analysis by secondary mass spectrometry confirmed the detection of these basic drugs. The results indicate that the mass spectrometry kit of this invention can remove impurities and extract and enrich target analytes, thereby improving detection sensitivity.

[0082] The above examples fully demonstrate that mass spectrometry kits can be used for the detection of complex body fluid samples, and can extract and enrich trace compounds in complex body fluid samples and achieve qualitative and quantitative analysis by coupling with mass spectrometry.

[0083] Example 5

[0084] Preparation method of a mass spectrometry kit for rapid simultaneous detection of multiple acidic compounds in body fluids:

[0085] (1) Mix 100 mg of polyethyleneimine, 150 mg of ethylene glycol dimethacrylate, 375 mg of dodecanol, 375 mg of methanol, 40 μL of purified water, and 10 mg of 2,2'-azobisisobutyronitrile. After ultrasonic dissolution and degassing, pour the mixture into a pretreated quartz capillary and heat it in a water bath at 60°C for 12 h to carry out the polymerization reaction. After in-situ polymerization of the monolithic material in the capillary, rinse away the unreacted substances with methanol to obtain an anion exchange capillary monolithic column, which is a functional material for specifically capturing analytes.

[0086] (2) The prepared anion exchange capillary monolithic column is combined with a quick connector and equipped with a corresponding disposable syringe, cleaning agent and sealing bag to assemble a mass spectrometry kit for rapid and simultaneous detection of multiple acidic compounds in body fluids.

[0087] Example 6

[0088] Preparation method of a mass spectrometry kit for rapid simultaneous detection of biomolecules containing cis-dihydroxy structures, such as nucleosides, carbohydrates, and glycoproteins, in body fluids:

[0089] (1) Take 100 mg of 4-vinylphenylboronic acid, 200 mg of N,N'-methylenebisacrylamide, 300 mg of n-propanol, 300 mg of methanol, 50 μL of purified water and 10 mg of 2,2'-azobisisobutyronitrile, mix them, dissolve them by sonication, degas them and pour them into a pretreated quartz capillary. Heat the capillary at 60°C for 12 h to carry out the polymerization reaction. After the monolithic material is polymerized in situ in the capillary, the unreacted substances are washed away with methanol to obtain the boric acid affinity capillary monolithic column, which is a functional material for specifically capturing analytes.

[0090] (2) The boric acid affinity capillary monolithic column was combined with a quick connector and equipped with a corresponding disposable syringe, cleaning agent and sealing bag to assemble a mass spectrometry kit for rapid and simultaneous detection of biomolecules containing cis-dihydroxy structures in body fluids.

[0091] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A method for preparing a mass spectrometry kit for rapid simultaneous detection of multiple components in body fluids, characterized in that, Includes the following steps: (1) Functional monomers, crosslinking agents, porogens and initiators are mixed and polymerized at a certain temperature, and then purified to obtain functional materials that specifically capture analytes; The functional monomer is one of potassium 3-sulfonylpropyl methacrylate, glycidyl methacrylate, 4-vinylphenylboronic acid, and polyethyleneimine; the crosslinking agent is one of N,N'-methylenebisacrylamide and ethylene glycol dimethacrylate; the porogen is at least one of water, methanol, cyclohexane, toluene, 1,4-butanediol, dodecanol, and n-propanol; and the initiator is one of 2,2'-azobisisobutyronitrile, ammonium persulfate, and benzoyl peroxide. The polymerization reaction is carried out in a reaction vessel comprising one of the following: stainless steel tube, glass tube, syringe needle, capillary, solid phase extraction column, magnetic nanomaterial, solid phase extraction pipette tip, thin layer plate, filter paper, filter membrane or glass monomer bottle. The reaction vessel is pretreated before the reaction. The reaction vessel is filled with a mixed solution of N,N-dimethylformamide and 3-(isobutenoyloxy)propyltrimethoxysilane of equal volume and reacted at 50~80℃ for 10~14h to attach double bonds to the inner wall of the vessel. (2) Using the functional material as the adsorbent for trace analytes, combine it with the general components of the mass spectrometry kit to complete the assembly of a mass spectrometry kit for rapid and simultaneous detection of multi-component compounds in body fluids.

2. The method according to claim 1, characterized in that, The specific operation of step (1) is as follows: the functional monomer, crosslinking agent, pore-forming agent and initiator are mixed, dissolved by ultrasound and degassed, and polymerized at a certain temperature; after the reaction is completed, the unreacted residues are washed away to obtain the functional material that specifically captures the analytes.

3. The method according to claim 1, characterized in that: If the target analyte detected by the mass spectrometry kit is basic, the selected functional monomer is potassium 3-sulfonopropyl methacrylate; if the target analyte is acidic, the selected functional monomer is polyethyleneimine; if the target analyte is a biomolecule containing a cis-dihydroxy structure, such as a nucleoside, sugar, or glycoprotein, the selected functional monomer is 4-vinylphenylboronic acid; if the target analyte is a small peptide, the selected functional monomer is glycidyl methacrylate; if the mass spectrometry kit requires the detection of a hydrophilic target analyte, the crosslinking agent is N,N'-methylenebisacrylamide; if the target analyte requires the detection of a hydrophobic target analyte, the crosslinking agent is ethylene glycol dimethacrylate.

4. The method according to claim 1, characterized in that: In step (1), the mass ratio of the polymer formed by the functional monomer and crosslinking agent to the porogen is in the range of 1:1.5 to 1:3.5; the amount of initiator added is 0.8% to 2% of the total mass of the functional monomer, crosslinking agent and porogen.

5. The method according to claim 1, characterized in that: In step (1), the polymerization reaction temperature is 50~80℃ and the reaction time is 10~14h.

6. The method according to claim 1, characterized in that, The specific operation of step (2) is as follows: using the functional material as the adsorbent for trace analytes, combining it with a quick connector, and equipping it with a corresponding disposable syringe, cleaning agent, and sealing bag, to assemble a mass spectrometry kit for rapid and simultaneous detection of multi-component compounds in body fluids.

7. A mass spectrometry kit for rapid and simultaneous detection of multiple components of compounds in body fluids, characterized in that: It is prepared by the method described in any one of claims 1 to 6.

8. A rapid mass spectrometry method for simultaneous detection of multiple components in body fluids, characterized in that, The mass spectrometry kit as described in claim 7 comprises the following steps: S1. A rapid mass spectrometry kit for simultaneous detection of multiple components in body fluids uses adsorption to quantitatively sample biological samples of body fluids to be tested. S2. After sampling, the mass spectrometry kit is cleaned with solvent to remove impurities and fix the target analyte. S3. Remove the portion of the mass spectrometry kit from which the target analyte is immobilized for subsequent detection. This portion can be used for immediate detection or for storing and transporting samples for detection when needed. S4. Elute the target analyte and proceed to mass spectrometry for detection and analysis.

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