868 results about "Casein" patented technology

The invention discloses a peptide with a bone strengthening effect as well as a preparation method and application thereof, and relates to the field of biology. The casein is used as a raw material, the high-activity calcium ion chelating peptide and the directional preparation technology thereof are provided, the technology has the advantages of being high in efficiency, low in cost, easy to operate, good in stability and the like, the high-activity calcium ion chelating peptide can be prepared on a large scale, the utilization value of the casein is improved, and a new way is provided for development of functional natural calcium supplement products.

The invention discloses a label cleaning type crispness increasing method for fried food, and belongs to the field of fried products. According to the preparation method, firstly, lentinus edodes stem nanocellulose is prepared by combining enzymolysis with a high-pressure homogenization method, and the lentinus edodes stem nanocellulose is compounded with casein, so that stable lentinus edodes stem nanocellulose-casein gel emulsion can be prepared; then the gel-like emulsion is applied to the paste-coated fried product, and the gel-like emulsion is used for forming pores in advance in the paste so as to achieve the crispness increasing effect, reduce the oil content and inhibit grease oxidation. The stable gel emulsion based on a natural formula can replace a commercial leavening agent to realize clean label type crispness enhancement, and the prepared paste can be applied to various paste fried foods and has a good market prospect.

The invention relates to the technical field of food processing, and discloses casein-tea polysaccharide-polyphenol composite nanoparticles and a preparation method of the casein-tea polysaccharide-polyphenol composite nanoparticles, and the preparation method comprises the following steps: mixing large yellow tea polysaccharide with a casein solution to obtain a CS / TPS mixed solution; performing glycosylation modification on casein in the CS / TPS mixed solution by adopting a Maillard reaction to prepare a casein-polysaccharide copolymer solution; the preparation method comprises the following steps: mixing a plurality of catechins, dissolving the catechins in ultrapure water to obtain a mixture stock solution, mixing the mixture stock solution and a casein-polysaccharide copolymer solution according to a volume ratio of 1: 16, stirring for 1 hour for self-assembly, pre-freezing the stirred solution at-80 DEG C, and freeze-drying for 48 hours to obtain CS-TPS-MIX nano-composite powder. According to the invention, a polyphenol delivery system with a dual antioxidant synergistic mechanism is constructed through a Maillard reaction mediated nano-composite technology, and the system is stable in structure, so that the release of polyphenol is more controllable.

The invention belongs to the technical field of food processing, and particularly relates to a curcumin-loaded protein-polysaccharide nanoparticle delivery system, a preparation method thereof and application of the curcumin-loaded protein-polysaccharide nanoparticle delivery system in neuroprotection. The preparation method comprises the following steps: mixing and dissolving sodium caseinate with one of glucan and pectin, hydrating at 0-4 DEG C for 12-24 hours, and performing ultrasonic and microwave treatment to obtain a pretreatment solution; adjusting the pH value, and heating the pretreatment solution to obtain covalent grafted particles; the protein-polysaccharide covalent grafted particles obtained in the second step are encapsulated with curcumin through a pH shift method, and the protein-polysaccharide-curcumin nanoparticles are obtained. The invention provides a method for carrying out Maillard reaction on sodium caseinate-polysaccharide under the assistance of ultrasound and microwaves, reaction parameters are optimized, and a preparation method of an efficient and stable curcumin delivery system and application of the curcumin delivery system in neuroprotection are constructed.

The invention belongs to the field of fermentation, and particularly relates to a complex microbial inoculant, original cheese, processed cheese with a blood sugar reducing function and preparation and application of the complex microbial inoculant. Wherein the processed cheese is prepared by the method comprising the following steps: mixing the components including raw cheese, sugarcane juice, corn oil, casein, concentrated milk protein, blueberry powder, mono / diglycerol fatty acid ester, sodium citrate, sodium tripolyphosphate, sodium hexametaphosphate, salt guar gum, potassium sorbate and water, and then emulsifying to obtain the processed cheese, the sugar cane juice, the corn oil, the casein, the concentrated milk protein, the blueberry powder, the mono / diglycerol fatty acid ester, the sodium citrate, the sodium tripolyphosphate, the sodium hexametaphosphate, the salt guar gum, the potassium sorbate and the water, the original cheese is prepared from a complex microbial inoculant, and the complex microbial inoculant comprises lactococcus lactis bacterial powder, lactococcus lactis subsp. Lactis bacterial powder, lactobacillus delbrueckii subsp. Lactis bacterial powder, lactobacillus plantarum bacterial powder and lactobacillus paracasei bacterial powder. The processed cheese provided by the invention has a better function of controlling blood sugar, and meanwhile, the processed cheese is fine and uniform in texture and has good smearing performance.

The invention provides a method for separating extracellular vesicles from a milk source, which comprises the step of adding a dispersing agent before adding a precipitator into the milk source to precipitate casein so as to reduce the wrapping of protein precipitation on the extracellular vesicles and improve the yield of the extracellular vesicles, thereby obtaining a milk source extract rich in the extracellular vesicles.

The invention belongs to the technical field of health-care products, and particularly relates to a formula helpful for improving sleep and a preparation method thereof. Comprising the following raw material components in parts by weight: 90-110 parts of spina date seed powder, 45-55 parts of casein hydrolysate peptide powder, 15-22 parts of gamma-aminobutyric acid, 18-22 parts of lucid ganoderma powder, 15-22 parts of lily powder, 18-20 parts of poria cocos powder, 12-15 parts of tea leaf theanine and 1-4 parts of lactobacillus plantarum JYLP-326. According to the formula for improving sleep provided by the invention, the limitation of an existing insomnia treatment means is broken through through a three-dimensional synergistic network of traditional Chinese medicines, probiotics and functional components, and the formula realizes systematic improvement on insomnia core symptoms through multi-channel synergy of nerve regulation, intestinal brain axis intervention and immune balance, namely rapid induction of sleep.

The invention relates to the technical field of biology, in particular to a DPP-IV inhibitory peptide extracted from buffalo cheese protein hydrolysate and application of the DPP-IV inhibitory peptide. An active component with the molecular weight smaller than 3 kDa is obtained through alkaline protease enzymolysis, ultrafiltration separation and LC-MS / MS identification, and the DPP-IV enzyme inhibitory activity IC50 value of the active component is 12.6 mg / mL. Four new DPP-IV inhibitory peptide fragments are found, are amino acid sequences in a sequence table SEQ ID NO: 1-4, have good DPP-IV inhibitory activity, and have good application prospects in being developed into functional foods or medicines for regulating blood sugar. In conclusion, the enzymolysis product and the peptide fragment can be prepared into antidiabetic drugs or functional foods, and have the advantages of being natural, safe and efficient. The invention provides a new strategy for high-valued utilization of buffalo milk resources and treatment of type 2 diabetes.

The invention provides a novel enterococcus strain Enterococcus qinghaiensis SCFF543 and an anti-oxidation application of the novel enterococcus strain Enterococcus qinghaiensis SCFF543, and belongs to the technical field of microorganisms. Wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 10, 2025, and the preservation number is CGMCC No.34843. The invention further discloses a preparation method of the strain. The strain is separated from homemade casein of herdsman families in Demaoha City in Haixi, Qinghai province, has the advantages of strong antioxidant capacity, no vancomycin resistance, strong adhesiveness and the like, and can be suitable for preparing probiotic products with antioxidant effects.

The invention belongs to the technical field of biology, and particularly relates to casein phosphopeptides for promoting absorption of calcium, iron and zinc minerals and improving bone health. Casein phosphopeptides containing high-activity peptide fragments are obtained through an optimization process, six mineral elements of calcium, magnesium, copper, iron, zinc and manganese serve as research objects, firstly, mineral utilization of CPP is evaluated through a mineral deficiency rat model, the content of Ca, Mg, Cu, Fe, Zn and Mn in thighbones is increased by 7.12%, 3.56%, 20.76%, 15.84%, 9.94% and 67.12% respectively when the CPP is eaten by 7mg / kg.d; secondly, screening a peptide fragment with calcium absorption promoting activity from the CPP by utilizing a molecular docking technology, and performing functional verification; the invention aims to reveal the action effect of CPP in mineral absorption and utilization from cell and animal levels, and provides a theoretical basis for functional development of CPP.

The invention belongs to the technical field of sleep-aiding oral liquids, and discloses a sleep-aiding nerve-calming oral liquid and a preparation process thereof, the oral liquid is composed of the following components: a plant fermentation broth, fructo-oligosaccharide, gamma-aminobutyric acid, casein hydrolysis peptide, a saussurea involucrata culture, and pyrroloquinoline quinone disodium salt; wherein the plant fermentation liquor is prepared from spina date seeds, poria cocos, lilies, Chinese dates, lotus seeds, lophatherum gracile, seville orange flowers, red roses with double petals, roselle, wheat, licorice roots, peach kernels, sweet-scented osmanthus and plant lactobacillus BXM2. The core component spina date seed saponin A is combined with gamma-aminobutyric acid to directly regulate the GABAergic nervous system, shorten the sleep latency and effectively help sleep and tranquilize the nerves; casein hydrolysis peptide promotes secretion of melatonin, and cooperates with the saussurea involucrata culture to resist oxidative stress, improve the sleep structure and restore circadian rhythm; small molecular active peptides and flavonoid glycosides are generated through fermentation, and anxiety mood, fatigue state and qi and blood circulation are improved; nano embedding and targeted delivery technologies are adopted in the whole process, the bioavailability is high, and the risk of drug dependence is avoided.

The invention belongs to the technical field of functional food processing, and particularly relates to a method for synergistically improving functional characteristics by utilizing casein peptide and hydroxytyrosol self-assembly. According to the research, casein peptide with an excellent antioxidant function is used as a raw material, and hydroxytyrosol is added to be self-assembled with the casein peptide, so that the antioxidant activity is synergistically improved in an in-vitro digestion stage. Compared with a traditional protein and polyphenol combination technology, the casein peptide and polyphenol self-assembly method is provided, the antioxidant activity after digestion is improved, no toxic chemical reagent is introduced or added, and the casein peptide and polyphenol self-assembly method is safer, more efficient, more environmentally friendly and more sustainable. The method can be widely applied to functional food or health care products.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention discloses a composite double-protein hypoglycemic peptide as well as a preparation method and application thereof, and belongs to the technical field of animal and plant source double-protein active peptides. The method comprises the following steps: performing computer simulation enzyme digestion and virtual screening by utilizing bioinformatics to determine a protein raw material and protease, performing enzymolysis on animal and plant double proteins (soybean protein and casein) serving as raw materials by adopting neutral protease, and performing separation, purification and structural identification, thereby obtaining the protein. The protein peptide fragment with high alpha-glucosidase inhibitory activity is obtained by virtually screening molecular docking, and the amino acid sequences of the protein peptide fragment are shown as SEQ ID No: 6, SEQ ID No: 9 and SEQ ID No: 24. The compound double-protein peptide sequence provided by the invention has an inhibiting effect on the activity of alpha-glucosidase, can be used for preventing and treating diabetes mellitus, and can be used for long-term health care or treatment of people with impaired glucose tolerance or diabetics as a functional food ingredient.

The invention discloses a bone-strengthening lactoprotein extract as well as a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains bone-strengthening active peptide FYPELFR. The preparation method specifically comprises the following steps: (1) pasteurization; (2) separating casein; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purifying and desalting; and (6) concentrating and drying. The bone-strengthening active peptide and the bone-strengthening lactoprotein extract containing the bone-strengthening active peptide can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonds, proliferation and differentiation of osteoblasts are promoted by activating a specific signal channel, proliferation of the osteoblasts is promoted, and the bone-strengthening active peptide and the bone-strengthening lactoprotein extract can be used for development of bone-strengthening functional food or drugs and have important significance on deep processing of cow milk.

The invention belongs to the technical field of nano-carriers, and particularly relates to a casein-based nano-carrier with slow release and pH / NIR dual response as well as a preparation method and application thereof, casein is combined with hollow copper sulfide nano-particles to load drugs, the nano-carrier with slow release and pH / NIR dual response is generated, and the drug loading rate is as high as 96.84%. Experiments show that the nano-carrier can accurately control drug release under specific pH and near-infrared light irradiation, so that the drug treatment efficiency is improved, and side effects on normal tissues are reduced.

The present invention relates to a method for in vitro sorting of target cells for cell therapy, belonging to the technical field of cell therapy. The present invention utilizes a washing solution containing EGTA, EDTA, α-casein, and prostaglandins to achieve a sorting method with high viable cell recovery efficiency, thereby enabling better cell therapy.

A nutritional composition comprising sn-2 palmitic acid and A2 beta-casein for use in improving the gut microbiota in a human subject.

The invention discloses a method for extracting a target product from a milk raw material, and relates to the technical field of food processing. According to the method, before strong anion exchange chromatography is carried out, a synergistic pretreatment step of ultrafiltration and conductivity adjustment is introduced, so that acceleration, synergism, quality improvement and cost reduction of the chromatography process are realized, and the technical bottlenecks of low speed, easiness in pollution, instability and the like of the traditional ion exchange chromatography during treatment of milk raw materials are overcome; and a solution is provided for high-efficiency, high-purity and large-scale production of the beta-lactoglobulin and the alpha-lactalbumin. According to the method, strong anion exchange chromatography and weak anion exchange chromatography can be combined, so that the extraction purity of beta-lactoglobulin and alpha-lactalbumin can be further improved; in addition, the method can synchronously realize high-efficiency extraction of various target substances such as casein micelles, lactose and milk mineral salts, so that the additional value and resource utilization efficiency of milk source byproducts are greatly improved.

The invention relates to an emulsifier composition for meat products as well as a preparation method and application of the emulsifier composition. The emulsifier composition disclosed by the invention is prepared from the following components in parts by weight: 5 to 10 parts of sodium caseinate, 0 to 5 parts of sucrose fatty acid ester, 10 to 15 parts of collagen, 5 to 10 parts of glutamine transaminase, 60 to 70 parts of maltodextrin, 0 to 5 parts of sodium pyrophosphate and 0 to 5 parts of trisodium phosphate. The invention relates to a meat product containing the emulsifier composition, wherein the amount of the emulsifier composition accounts for 0.6%-1% of the total weight of the meat product. According to the invention, the obvious compounding effect among the monomer emulsifier, collagen, phosphate and glutamine transaminase is fully utilized, the functional complementation principle of various raw materials is exerted, and the synergistic interaction effect is generated. On the basis of enhancing the taste of the meat product, the oil and water retention property is improved to a greater extent, so that the yield is improved, and the production cost is reduced.

The invention relates to the technical field of enzyme-linked immunosorbent assay, and discloses a quality control serum diluent as well as a preparation method and application thereof, and the quality control serum diluent is prepared from the following raw materials: boric acid, borax, sodium caseinate, liquid gelatin, aminopyrine, an osmotic pressure stabilizer and bovine serum. The quality control serum diluent prepared by adopting the formula has no potential interfering substances (such as formaldehyde and thiomersalate), has excellent thermal stability and long-term stability, is applied to quality control serum dilution, is beneficial to improving the detection accuracy, ensures that the characteristics of the diluent meet the requirements when the diluent is used every time, and provides stable reference data for the detection process.

The invention discloses purple perilla plant milk rich in strontium element and a preparation process thereof. The purple perilla plant milk is prepared from the following raw materials: shelled purple perilla seeds, shelled flaxseeds, peeled cyperus esculentus, sesame, soybeans, peas and strontium-rich water. The preparation method mainly comprises the following steps: raw material pretreatment, fine wet grinding, filtering and deslagging, high-pressure homogenization and refinement, ultrahigh-temperature instantaneous sterilization and sterile filling. The method ensures the fine taste, stability and safety of the product. The plant milk is rich in strontium element and high-quality plant protein, does not contain lactose and cholesterol, and has the advantages of bone health maintenance, low sensitivity and the like. The problems that casein allergy and lactose intolerance risks exist in traditional animal milk, and existing plant milk is low in protein content, unbalanced in nutrition, dependent on additives and single in function are solved.

This invention belongs to the field of nutritional composition technology, specifically disclosing a composition for promoting brain cognitive development, its preparation method, and its application. The composition for promoting brain cognitive development provided by this invention includes transforming growth factor, casein phosphopeptide, immunoglobulin, and sn-2 DHA, and further specifies that the mass ratio of transforming growth factor, casein phosphopeptide, immunoglobulin, and sn-2 DHA is (0.1~1.4) ng:(40000~160000) mg:(20000~60000) mg:(20000~70000) mg. In this invention, the preferred ratio of transforming growth factor, casein phosphopeptide, immunoglobulin, and sn-2 DHA can achieve the effect of promoting brain cognitive development through a multi-dimensional synergistic mechanism.

The invention provides a method for separating beta-casein from a dairy product, a method for separating kappa-casein and a casein mixture. The method for separating the beta-casein from the dairy product comprises the following steps: separating whey and casein in skimmed milk to obtain a casein concentrated solution; carrying out low-temperature microfiltration-percolation treatment on the casein concentrated solution at the temperature of 10 DEG C or below to obtain beta-casein permeate and a by-product of which at least part of beta-casein is removed; and heating the beta-casein permeate to realize self-association of the beta-casein permeate, and then carrying out ultrafiltration-percolation to obtain the beta-casein. The influence on the structure and performance of the beta-casein in the separation process is reduced, and the purity of the separated beta-casein product is improved.

The invention discloses a preparation method of a metagen microcapsule. The metagen microcapsule is prepared from a wall material and a core material through electrostatic spinning, the core material comprises a metagen compound coating at least two layers of chitosan-casein / pectin compound nanoparticles; the wall material comprises polyvinyl alcohol and polycyclohexane; the mass ratio of the metagen compound to the polyvinyl alcohol to the polycycloethane is (4-7.5): (0-3): (0-7). The three materials of chitosan, casein and pectin have excellent affinity with human tissues and cells, the survival rate of probiotics can be remarkably increased, and accurate controlled release of active substances is achieved. By optimizing a coating process and adopting an electrostatic spinning technology, the probiotics coated with chitosan, casein and pectin nanoparticles are prepared into the probiotic composite fiber, so that the storage capacity and the release efficiency of the probiotics are effectively improved, and meanwhile, the stability and the consistency of the product quality are ensured.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention provides a long-acting antibacterial / antioxidant biomass-based essential oil microcapsule and a preparation method thereof. The preparation method comprises the following steps: firstly, uniformly stirring and mixing a chitosan stock solution and a casein stock solution, adjusting the pH value of a mixed system, and promoting the formation of nanoparticles through reverse charge neutralization of casein and chitosan; centrifuging the mixed solution, collecting a supernatant, slowly dripping essential oil into the supernatant, stirring and emulsifying, and ultrasonically preparing an emulsion; and finally, adding a natural cross-linking agent for cross-linking, and filtering and drying after reaction to obtain the long-acting antibacterial / antioxidant biomass-based essential oil capsule. The method does not need an additional emulsifier, is simple in process and low in cost, and is suitable for the fields of drug delivery, cosmetics and food preservation. The prepared microcapsule has the advantages of better stability, high coating rate, good slow release performance, long-acting antibacterial / antioxidant property and the like.

The invention belongs to the technical field of food or medicine, and particularly relates to a preparation method of nattokinase sustained-release microspheres, which comprises the following steps: mixing an emulsifier span-80 with liquid paraffin to obtain an oil phase; adding chitosan into an acetic acid solution to prepare a chitosan water solution as a water phase; dropwise adding the water phase into the oil phase to prepare W / O type emulsion, dropwise adding a cross-linking agent A into the emulsion to obtain chitosan microspheres, and taking a chitosan microsphere nattokinase solution and a cross-linking agent B to obtain nattokinase-chitosan core microspheres; mixing the nattokinase-chitosan core microsphere with a cortisol aptamer solution, and incubating to enable the aptamer to be combined on the surface of the nattokinase-chitosan core microsphere; and adding a casein-PEG copolymer solution and a cross-linking agent C into the microspheres to obtain the cortisol response type intelligent switch microspheres. The nattokinase release amount can be dynamically adjusted day and night, the circadian thrombus risk rhythm is matched, the anti-thrombus effect is remarkably improved, side effects are reduced, and the nattokinase can be widely applied to the fields of functional food and drugs.

The invention discloses a preparation method and application of milk-derived peptide capable of efficiently promoting calcium absorption and bone formation, and relates to the field of dairy product processing. Casein in milk is hydrolyzed through papain or protease of similar enzymolysis sites, a primary product is obtained through concentration, and a final product is obtained through subsequent storage or drying and other technological operations. The obtained hydrolysate has outstanding functions of promoting calcium absorption and bone formation, especially in bone formation.

The invention discloses an elastic chewable nicotine oral product and a preparation method thereof. The elastic chewable nicotine oral product comprises a nicotine source and an additive, the nicotine source is dissolved in the mixed solution of the additive, so that the nicotine oral product forms a soft elastomer, and the nicotine is slowly released in the oral cavity through chewing; the nicotine source is extracted or chemically synthesized from tobacco; the additive comprises a gelling agent, and the gelling agent comprises one or a combination of more of gelatin, agar, konjac polysaccharide, xanthan gum, pectin, carrageenan, sodium alginate, semen plantaginis polysaccharide, guar gum, gellan gum, curdlan, whey protein, casein or soybean protein. The gel is added into the carrier to realize physical embedding of the nicotine, the elastic and chewable oral nicotine product can be obtained without bonding fixation, and the nicotine is released through gel swelling-diffusion regulation, so that the oral nicotine product has no residue in the oral cavity and does not pollute the package when being used, and the purposes of dynamically releasing the nicotine and improving the consumption experience are realized.