520 results about "Casein" patented technology

The invention discloses a peptide with a bone strengthening effect as well as a preparation method and application thereof, and relates to the field of biology. The casein is used as a raw material, the high-activity calcium ion chelating peptide and the directional preparation technology thereof are provided, the technology has the advantages of being high in efficiency, low in cost, easy to operate, good in stability and the like, the high-activity calcium ion chelating peptide can be prepared on a large scale, the utilization value of the casein is improved, and a new way is provided for development of functional natural calcium supplement products.

The invention provides a novel enterococcus strain Enterococcus qinghaiensis SCFF543 and an anti-oxidation application of the novel enterococcus strain Enterococcus qinghaiensis SCFF543, and belongs to the technical field of microorganisms. Wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 10, 2025, and the preservation number is CGMCC No.34843. The invention further discloses a preparation method of the strain. The strain is separated from homemade casein of herdsman families in Demaoha City in Haixi, Qinghai province, has the advantages of strong antioxidant capacity, no vancomycin resistance, strong adhesiveness and the like, and can be suitable for preparing probiotic products with antioxidant effects.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention relates to the technical field of food, in particular to yoghourt with high casein content and a preparation method of the yoghourt. The preparation method of the yoghourt provided by the invention comprises the following steps: mixing raw milk, a membrane separation casein concentrated solution and inorganic salt; carrying out hydrodynamic cavitation treatment on the mixed raw material solution, then inoculating a leavening agent for fermentation, and when the fermentation is carried out to a certain pH value, applying ultrasonic treatment while the fermentation is carried out. The high-casein-content yoghurt prepared by the method provided by the invention has proper viscosity, hardness and cohesiveness, smooth and stable texture, better water binding capacity, lower whey precipitation rate, better stability and excellent sensory evaluation performance such as taste, smell and the like. The yoghourt provided by the invention has the characteristics of high protein content and high casein content, has the characteristics of natural low fat, high calcium and strong satiety due to the high casein content, and can better meet the requirements of people with body building, fat reducing and calcium supplementing.

Provided herein are formulations for oral delivery or administration of therapeutic nucleic acids. An oral formulation can include: a nucleic acid; at least one casein protein; and a chitosan. In some embodiments, the formulations are liposome-free formulations having no cationic lipids typically used as transfection reagents in conventional nucleic acid formulations for oral delivery. The formulations provided herein find use in treating a condition associated with inflammation and / or fibrosis, or a cardiometabolic disorder by oral administration.

The invention discloses a nutritional composition for promoting central nervous system development and application. The invention provides a nutritional composition for promoting the development of a central nervous system. The nutritional composition comprises the following necessary components: a breast milk oligosaccharide component and a casein phosphopeptide component, moreover, in the composition, the mass ratio of the breast milk oligosaccharide component to the casein phosphopeptide component is (1-100): (500-1). According to the invention, the breast milk oligosaccharide component and the casein phosphopeptides are matched to prepare the nutritional composition, and the nutritional composition can significantly promote nerve development such as neuronal maturation, synaptic generation and myelin sheath formation.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention relates to the technical field of biology, in particular to a method for extracting PDRN from plants and application. In the preparation process, the casein is added, so that the casein and the PDRN are competitively combined with impurities in plants; enzyme is added for enzymolysis treatment, so that subsequent centrifugation and other operations are facilitated; the impurities are further removed by adding Tris-HCl, NaCl, CTAB (cetyltrimethyl ammonium bromide) and EDTA (ethylene diamine tetraacetic acid) and controlling each concentration. By adopting the method provided by the invention, the A260 / A280 of the PDRN extracted from the plant is greater than or equal to 1.69, the A260 / A230 is greater than or equal to 1.55, the PDRN has the advantage of high purity, and the obtained PDRN has the effects of resisting aging, repairing a barrier, resisting inflammation and the like, and has a good application prospect.

The invention discloses a yak milk casein ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and relates to the technical field of medicines and health-care products. The preparation method of the yak milk casein ACE inhibitory peptide comprises the following steps: taking yak milk casein as a substrate, adding NaCl and cane sugar to prepare a fermentation culture medium, inoculating a high-yield protease strain into the fermentation culture medium for constant-temperature fermentation, deactivating enzyme after fermentation, centrifugally collecting supernate, and freeze-drying to obtain the yak milk casein ACE inhibitory peptide. The yak milk casein ACE inhibitory peptide oral liquid contains sodium carboxymethyl cellulose, cane sugar, citric acid and yak milk casein ACE inhibitory peptide, the invention also provides an application of the yak milk casein ACE inhibitory peptide in preparation of drugs or health-care products for assisting in lowering blood pressure. The yak milk casein ACE inhibitory peptide disclosed by the invention has an antihypertensive function and good palatability, fills the blank of a yak milk deep processing product market, and provides a technical path for extension of yak milk from a traditional dairy product to the biological medicine field.

The invention discloses a polypeptide with blood pressure lowering and oxidation resisting effects as well as a related product and application thereof, and relates to the technical field of biology. According to the invention, casein is taken as a raw material, and the milk-derived active peptide ALPQY is successfully obtained through multi-enzyme hydrolysis, separation, purification and identification. In-vitro activity evaluation results show that the polypeptide not only has strong ACE inhibitory activity, but also can effectively scavenge free radicals; the ACE inhibition IC50 value is 35.56 mu M, the free radical scavenging TEAC value is 1.83 mu mol TE / mu mol, and compared with similar polypeptides, the polypeptide has better ACE inhibition effect and free radical scavenging effect. In-vivo antihypertensive evaluation is carried out through a spontaneous hypertension rat model, it is also shown that the compound has a good antihypertensive effect, and the compound can be used for preparing products related to antihypertensive and / or antioxidation.

The invention provides a teak camel moth artificial feeding feed and a teak camel moth artificial feeding method, the feed comprises the following components by weight: 8% of wheat germ powder, 4% of soybean meal, 4% of yeast powder, 2% of casein, 2% of sucrose, 1.8% of agar powder, 1.5% of cellulose, 0.8% of Webster salt, 0.6% of vitamin C, 0.2% of sorbic acid, 0.3% of methyl p-hydroxybenzoate, 0.1% of cholesterol, 0.1% of choline chloride, 0.05% of inositol, 0.01% of folic acid, and the balance of water. And the balance of deionized water. The invention further provides an artificial feeding method of teak camelmoths, and the artificial feeding method comprises the steps of 1, adult mating and egg laying, 2, egg disinfection and incubation and 3, larva rearing. The feed does not contain host material teak leaves, and full-artificial continuous large-scale feeding of the teak camelmoths can be achieved.

The invention relates to an application of a bioactive peptide YPFPGPIH derived from bovine casein in the aspect of anti-inflammation or immunoregulation. The peptide can be combined with TLR2 and TLR4 to inhibit the activation of NF-kappa B and MAPK signal channels, so that the levels of inflammatory factors such as IL-1beta, TNF-alpha and NO are remarkably reduced, the expression of IL-10 is up-regulated, and inflammation balance regulation is realized; the body inflammatory response is improved, and the immune organ injury is recovered. The peptide can be used for preparing anti-inflammatory drugs, immunomodulatory functional foods and nutritional supplements, and has good safety and application prospects.

The invention provides a casein-sodium alginate compound as well as a preparation method and application thereof, and relates to the field of foods, health foods or medicines. The preparation raw materials comprise casein, sodium alginate and a deep-eutectic solvent, and the dosage ratio of the casein to the sodium alginate to the deep-eutectic solvent is (1.0-2.0) g: (1.0-3.0) g: (30-70) mL. The sodium alginate is combined with the casein through the electrostatic adsorption effect, self-assembly of the casein and the sodium alginate is further promoted through the deep-eutectic solvent, the compound is formed, and the emulsifying property and the solubility of the emulsion based on the compound are remarkably improved. When the casein-sodium alginate compound provided by the invention is used as an emulsifier, the stability of an emulsion can be remarkably improved, and when the emulsion is used for loading lipophilic active ingredients, the biological activity of the lipophilic active ingredients can be remarkably improved compared with an emulsion prepared from the casein-sodium alginate compound without a deep-eutectic solvent.

Disclosed in the present invention is a monodisperse nanocalcium carbonate-based foliar calcium fertilizer, comprising the following raw materials in parts by mass: 1-20 parts of a monodisperse nanocalcium carbonate filter cake, 0.05-0.9 parts of ethoxy-modified polytrisiloxane, 0.01-0.8 parts of modified casein, 0.01-0.8 parts of an enzyme, 0.05-0.9 parts of a sugar alcohol, and 10-180 parts of water. After being diluted by means of water such that the solid content of calcium carbonate is 0.5-2%, the foliar calcium fertilizer is used for foliar calcium supplementation of various crops and fruit and vegetable crops. The novel foliar calcium fertilizer is safe and non-toxic, can be directly sprayed onto foliar and fruit surfaces of crops and fruits and vegetables, effectively ensures calcium absorption and utilization in crops, achieves the effect of increasing yield and increasing income, and can also improve the yield of fruits and vegetables.

The application discloses a composition, which comprises hydrolyzed protein; the hydrolyzed protein comprises hydrolyzed whey protein and hydrolyzed casein protein, the weight ratio of the hydrolyzed whey protein and the hydrolyzed casein protein is (70-95):(5-30), the amino acid nitrogen content in the hydrolyzed protein is (1.0-3.0) g / 100 g, the proportion of protein hydrolysate with a relative molecular mass distribution <1000 in the hydrolyzed protein is 34-70%, the peptide content (calculated on a dry basis) in the hydrolyzed protein is not less than 33 g / 100 g, and the total free amino acid content in the hydrolyzed protein is not less than 1 g / 100 g. The composition can effectively treat diarrhea and is suitable for people with gastrointestinal dysfunction and severe patients with diarrhea symptoms.

The invention relates to a truncated casein polypeptide (TCP), said truncated casein polypeptide being preferably selected from alpha S1-casein (αS1-casein), alpha S2-casein (αS2-casein), beta-casein (β-casein), or kappa-casein (κ-casein) polypeptide or a mixture thereof, and compositions comprising thereof. The invention further relates to a dairy product or dairy-like product comprising said truncated casein polypeptide (TCP). Such composition can also be used for the preparation of nutritional product. Finally, the invention further relates to methods of making the truncated casein polypeptide (TCP) of the invention and methods of making the dairy product or dairy-like product of the invention.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

This application discloses a sludge solidification agent, its preparation method, and its application, belonging to the field of sludge solidification technology. The sludge solidification agent comprises solid waste composite powder, a composite activator, and a microbial self-healing agent. The microbial self-healing agent is a chitosan-bentonite microcapsule loaded with Bacillus pasteurellii. The Bacillus pasteurellii is encapsulated within a protective layer composed of chitosan and bentonite using microencapsulation technology, thus maintaining dormancy and high activity during long-term storage. Through dual modification of the key components of the activator (hydrophobic microcapsules encapsulating water glass and surface passivated gypsum), the problems of pre-reaction and clumping failure due to moisture absorption in single-component powders are solved. The introduction of specific organic regulators (xanthan gum and casein) significantly improves the solidification effect on high-organic-matter sludge. Therefore, the sludge solidification agent provided in this application can achieve long-term microbial survival, dry-state stability of the activator, and efficient solidification of high-organic-matter sludge.

The present application provides a micellar casein powder, a preparation method and system thereof and a food, wherein the calcium-phosphorus ratio of the micellar casein powder is (1-1.2):1. The micellar casein powder of the present application has a calcium-phosphorus ratio similar to breast milk, low mineral content, high solubility, high nutritional value and is suitable for large-scale production.

The application discloses a preparation method of an immune protein extract of colostrum with tumor inhibition efficacy, and comprises the following steps: (1) raw material selection; (2) flocculation of casein; (3) sugar removal; (4) ultrafiltration membrane filtration; (5) directional hydrolysis; (6) cation exchange resin adsorption; (7) desalination; and (8) freeze drying. The extract of the application is rich in hydrophobic amino acid and alkaline amino acid hydrolysis fragments of the immune protein of colostrum, and has stronger antioxidant, immune enhancement and tumor inhibition activities. Different from common immune proteins of colostrum, the extract plays the anti-tumor activity as a result of the combined action of its double effects of immune enhancement and tumor cell growth inhibition.

The application discloses a plant lactobacillus and application thereof in improving stretchability of acid coagulated cheese. Lactiplantibacillus plantarum The plant lactobacillus is named as plant lactobacillus (Lactobacillus plantarum) B11, is preserved in China Center for Type Culture Collection, has a preservation number of CCTCC NO: M 2025778 and a preservation address of Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The strain provided by the application can be directionally fermented to produce viscous exocellular polysaccharide and antibacterial substances. The acid whey fermented by the strain is combined with phosphate and sodium caseinate quality improver, so that the stretchability of the acid coagulated cheese is improved, the product is inhibited from being mildewed in the airing and storage processes, and meanwhile, the product quality safety can be ensured. The problems of stretching difficulty, low product yield and easy mildewing in the airing and storage processes in the prior art are solved.

The present invention refers to an iron (III) caseinsuccinylate characterised by a content of iron comprised between 4.5 % and 7 % by weight, and by a solubility in water approximately greater than 92%. :The present invention further refers to a method for preparing iron (III) caseinsuccinylate.

The invention provides a method for detecting enzyme activity of papain in a hypersensitive protein complex enzyme, which comprises the following steps: mixing the hypersensitive protein complex enzyme with an L-cysteine hydrochloride buffer solution to obtain a sample solution to be detected; adding the to-be-tested sample solution into a substrate solution containing casein, and testing a change curve of a voltage value of the to-be-tested sample solution along with time under the condition that the casein can be decomposed by papain; and determining the enzyme activity of the papain in the hypersensitive protein compound enzyme according to the change curve of the voltage value of the to-be-detected sample solution along with time. The method can solve the problem that the enzyme activity of papain in the hypersensitive protein complex enzyme cannot be accurately detected in the prior art.

The invention discloses a fish hydrolyzed collagen peptide-based flavor beverage and a preparation method thereof, and relates to the technical field of food beverages. The flavored beverage comprises the following raw materials: fish hydrolyzed collagen peptide, a compound flavor modification stabilizer, a compound sweetening agent, concentrated fruit and vegetable juice, casein phosphopeptides, VC magnesium phosphate, zinc lactate, a low-nucleic-acid yeast extract, sodium hyaluronate, an acidity regulator and purified water. The compound flavor modification stabilizer is matched with the low nucleic acid yeast extract to form synergistic flavor regulation and control, the hydrophobic bitter peptide fragment of the fish hydrolyzed collagen peptide and the fishy smell characteristic group are wrapped from the molecular level, and meanwhile, the soft modification on the sensory level is realized through the gamma-aminobutyric acid; the effect of debitterizing and deodorizing without adding artificial essence and a large amount of cane sugar is achieved, the product flavor is natural and harmonious, the sour and sweet taste is palatable, the beverage completely conforms to the current healthy consumption trend of low sugar and no artificial addition, and the problem that the beverage has flavor defects is solved.

The invention relates to a process for dephosphorylation of skim milk, ultra-filtered milk (UF milk) or micellar casein isolate (MCI) comprising (i) acidification of a skim milk, UF milk or MCI preferably not lower than pH 6.0, preferably between 6.0 and 6.7, (ii) cooling the acidified skim milk, UF milk or MCI to the temperature between 0° C. and 15° C., (iii) adding gluconate and / or maleate to the cooled skim milk, UF milk or MCI and, (iv) washing the skim milk, UF milk or MCI, to remove phosphorus, thus dephosphorylating the skim milk, UF milk or MCI, preferably to an extent that the total phosphorous content of the skim milk, UF milk or MCI is reduced with at least 20%, preferably 20-40%, more preferably 30-40% compared to the material provided to step (i). The invention also relates to dephosphorylated MPC, MPI or MCI obtainable by the process of the invention, and to a liquid heat-sterilized enteral nutritional composition comprising 2.0-3.0 kcal / ml wherein 16-35 en % is provided by protein, the combination of caloric content and relative protein caloric content selected such that there is 10-18 g / 100 ml protein, preferably 12-18 g / 100 ml protein in the composition, wherein the protein comprises micellar casein (MC), whey protein (WP) and optionally caseinate (CAS), wherein there is at least 70 wt % MC and less than 15 wt % WP, based on total protein content, and wherein the composition has a total amount of phosphorous less than 192 mg / 100 ml and / or 30-80 mg / 100 kcal, preferably at least 30-80 mg / 100 kcal, most preferably less than 192 mg / 100 ml and 30-80 mg / 100 kcal.

The invention discloses a crispness increasing method for crispy flour products, and belongs to the field of crispy products. According to the invention, the casein solution and the wheat protein solution are firstly prepared and are used as dispersion phases to prepare the gel-like emulsion with the oil phase of 60-80%, so that the freezing resistance of the gel-like emulsion is improved, and then the gel-like emulsion is used as a pore-forming agent to be added into the paste, so that a new way for preparing the crispy flour product is developed. The gel-like emulsion is applied to the crispy flour product to replace a traditional leavening agent, so that the greenness of the manufacturing method of the crispy flour product can be realized, the oil content and the oil oxidation degree of the crispy flour product are reduced, the moisture and network integrity of dough is better maintained, the operation efficiency and convenience are improved, and the production cost is reduced. The color and luster of the crispy flour product can be effectively regulated and controlled, and technical innovation and quality improvement in the field of the crispy flour product are expected to be promoted.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.

This invention discloses a method for producing high-quality sodium caseinate, belonging to the field of food engineering technology. The method includes enzymatic modification, low-temperature alkali conversion, ceramic membrane microfiltration, ultrafiltration desalting and concentration, homogenized spray drying, and fluidized bed agglomeration granulation. Enzymatic modification uses a complex enzyme of endopeptide and phosphatase to hydrolyze bitter peptides and phytic acid at 45–55℃; low-temperature alkali conversion involves gradient alkali addition at 15–25℃ to avoid thermal damage and Maillard reactions; ultrafiltration employs equal-volume dialysis, with water added until the conductivity is ≤500 μS / cm, desalting and removing small-molecule bitterness; finally, agglomeration granulation is achieved by fluidized bed spraying with 0.1%–0.5% lecithin. This invention completely abandons the acid precipitation-alkali dissolution process, coupling enzymatic modification with all-physical membrane separation and purification to produce sodium caseinate that is white in color, pure in flavor, dissolves rapidly in cold water in ≤30 seconds, has an ash content ≤3.5%, reduces wastewater discharge by more than 90%, is environmentally friendly, and suitable for large-scale production.

The invention belongs to the technical field of intelligent packaging films, and particularly relates to a casein-copper ion bionic nano-enzyme indicating film for meat freshness monitoring and a preparation method thereof.The method comprises the steps that casein is dissolved in an alkaline solution under the water bath condition, stirring is conducted overnight, and a casein stock solution is prepared; and then, uniformly stirring and mixing the casein stock solution and the copper ion aqueous solution, adjusting the pH value of the mixed system, and promoting the formation of the casein-copper ion bionic nano-enzyme mixture through the coordination effect of copper ions and casein. And then freeze-drying the solid obtained by centrifuging the mixture, and grinding with mortar. And finally, mixing and stirring the Cu-BAN powder, glycerol and a polyvinyl alcohol solution, pouring into a culture dish after reaction, and drying to obtain the Cu-BAN indicating film for meat freshness detection. The method is mild in reaction condition, simple in preparation process, low in cost, high in safety and environmental friendliness and capable of being applied on a large scale.