68 results about "Lactalbumin" patented technology

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention discloses a method for extracting a target product from a milk raw material, and relates to the technical field of food processing. According to the method, before strong anion exchange chromatography is carried out, a synergistic pretreatment step of ultrafiltration and conductivity adjustment is introduced, so that acceleration, synergism, quality improvement and cost reduction of the chromatography process are realized, and the technical bottlenecks of low speed, easiness in pollution, instability and the like of the traditional ion exchange chromatography during treatment of milk raw materials are overcome; and a solution is provided for high-efficiency, high-purity and large-scale production of the beta-lactoglobulin and the alpha-lactalbumin. According to the method, strong anion exchange chromatography and weak anion exchange chromatography can be combined, so that the extraction purity of beta-lactoglobulin and alpha-lactalbumin can be further improved; in addition, the method can synchronously realize high-efficiency extraction of various target substances such as casein micelles, lactose and milk mineral salts, so that the additional value and resource utilization efficiency of milk source byproducts are greatly improved.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention discloses a preparation method and application of an anti-continuous cropping microbial fertilizer, and aims to solve the problems of aggravation of crop diseases and insect pests and reduction of yield and quality caused by continuous cropping planting. The preparation method comprises the following steps: activating and uniformly mixing bacillus subtilis and bacillus licheniformis, and adding an alpha-lactalbumin solution and a calcium chloride solution to prepare a bacillus aggregate; adding bentonite into the polytrehalose solution, and performing ultrasonic treatment on the mixture and the humic acid solution to prepare a modified polytrehalose-bentonite compound; uniformly mixing acremonium terricola, trichoderma harzianum and a bacillus aggregate, and adding a wall material solution to prepare a compound bacterium microcapsule preparation; and finally, mixing the microcapsules with the corn straw biochar, the chitosan oligosaccharide and the like to obtain a finished product. Through calcium ion induction and humic acid modified embedding, the strain stability and viable count are improved, soil organic matter and nitrogen phosphorus and potassium nutrients can be activated, pathogenic bacteria are inhibited, the crop rhizosphere environment is improved, the growth index and quality of red kidney beans and potatoes are effectively improved, and the method is suitable for continuous cropping resistant planting of crops.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

The present invention provides a method of predicting the outcome of a treatment of a cancer or a metabolism-related disease in a subject, the treatment comprising administering to the subject a complex comprising a polypeptide having a naturally occurring alpha-lactalbumin sequence, or a functional variant thereof; or a peptide comprising up to 50 amino acids of the alpha-helical domain of the polypeptide; and a fatty acid or lipid or a salt thereof; wherein the method comprises the steps of: a) evaluating the regulation of cancer genes in the subject after administration of the complex, and b) correlating the regulation of the genes to the outcome of the treatment wherein the positive outcome of the treatment is negatively correlated to the level of regulation of those genes.

A method for producing a gold colloid-labeled antibody suspension, comprising: a labeling step of obtaining a mixture by mixing a gold colloid solution and an antibody solution to obtain a gold colloid-labeled antibody in the mixture; a centrifugation step of centrifugating the mixture to obtain a precipitate of the gold colloid-labeled antibody and removing the supernatant containing antibody that does not have gold colloid adsorbed; and a suspension step of suspending the precipitate of the gold colloid-labeled antibody in a suspension solution containing lactalbumin to obtain a gold colloid-labeled antibody suspension.

The invention belongs to the technical field of protein purification, and particularly relates to a chromatography medium, a preparation method of the chromatography medium and application of the chromatography medium to extraction of alpha-lactalbumin. The chromatography medium provided by the invention takes agarose as a matrix, and the surface of the chromatography medium is modified by a ligand through a glucan spacer arm; wherein the molecular weight of the glucan is 20 kDa to 500 kDa, the ligand comprises propylene diamine, and the density of the ligand in the chromatography medium is 200 [mu] mol / mL to 300 [mu] mol / mL. The invention also provides a preparation method of the material, and the material can be obtained by sequentially carrying out epoxy activation, glucan modification, secondary activation and ligand bonding on the matrix. When the chromatography medium provided by the invention is used for extracting alpha-lactalbumin from whey, the chromatography medium has the advantages of high loading capacity, high loading flow rate and efficient regeneration, and meets the requirements of industrial production.

The invention provides a composition for chronic wound treatment and a preparation method thereof, and relates to the field of medicines. The mask is prepared from the following raw materials in percentage by mass: 20 to 30 percent of methacrylic acid hyaluronic acid, 20 to 30 percent of metacrylic acid ester modified silk fibroin, 40 to 50 percent of carboxymethyl chitosan, 1 to 2 percent of photoinitiator and 5 to 10 percent of hydrogen sulfide sustained-release microspheres, the hydrogen sulfide sustained-release microsphere is a core-shell microsphere which takes GYY4137-PLGA (poly (lactic-co-glycolic acid)) nanoparticles as a core layer and EGCG (epigallocatechin gallate)-whey protein as a shell layer. The composition provided by the invention can slowly release H2S gas, promote angiogenesis of chronic wounds and promote wound healing.

The present invention relates to the field of nutritional compositions, methods of providing nutritional compositions and uses thereof. More particularly, the present invention relates to a ready-to-eat powdered infant formula or ready-to-eat powdered growth formula exhibiting desirable gastric digestion characteristics. There is provided a nutritional composition comprising casein, whey protein comprising alpha-lactalbumin (aLac) and beta-lactoglobulin (bLac), and protein-coated fat droplets wherein the composition has a protein content of from 5% to 20% (w / w) based on total solids; the bLac content is at least 3% (w / w) based on the total protein level, and the aLac + bLac content is at least 25% (w / w) based on the total protein level; the fat content is in the range of 10% to 50% (w / w) based on total solids; the fat droplets are coated with an average protein load of 2 to 10 mg / m2, and wherein the weight ratio of casein: (aLacc + bLac) on the fat droplets is at least 4 times the weight ratio of casein: (aLacc + bLac) in the total composition; the level of sphingomyelin per 100 g of the composition is between 70 mg and 200 mg; the level of phospholipids per 100 g of the composition is between 280 mg and 950 mg.

The application discloses a preparation method of a target substance and related products and application thereof, and relates to the field of food processing. The application adjusts the pH and conductivity of natural whey to change the whey characteristics, and uses an anion exchange chromatography method to realize high-purity separation of alpha-lactalbumin. The method requires a neutral and slightly alkaline environment, which is beneficial to maintaining the activity of alpha-lactalbumin. The obtained alpha-lactalbumin is rich in alpha-helix structure and has high similarity with the natural structure of alpha-lactalbumin. The separation mode of the anion exchange chromatography is a flow-through mode, which significantly improves the total amount of whey protein treated by ion exchange method, shortens the processing time of each batch, and improves the production efficiency. The method has important significance for the industrialized separation and production of alpha-lactalbumin.

The invention provides a method for preparing a biologically active complex, said method comprising dissolving a mixture of a polypeptide element, such as alphalactalbumin or fragments thereof, in powder form and oleic acid or a pharmaceutically acceptable salt thereof also in solid form, in an aqueous solvent comprising a mixture of at least two and preferably three salts, wherein the method is carried out at moderate temperatures. The preparation does not require extensive heating and so is simple and efficient to carry out.

The invention provides a milk flavor essence and a preparation method thereof. The method comprises the following steps: preparing an activated bacterium solution from German-type lactobacillus bulgaricus subspecies; anhydrous cream and whey powder are prepared into a fermentation substrate; adding the activated bacterial liquid into the fermentation substrate, and performing sealed fermentation to obtain a fermentation product; and carrying out first sterilization treatment on the fermentation product, carrying out centrifugal separation, and taking supernate as the milk-flavored essence. Whey powder is added when a fermentation substrate is prepared, the whey powder is rich in whey protein, lactose, vitamins and mineral substances, and glycometabolism, protein metabolism and lipid metabolism pathways are activated in the fermentation process, so that a fermentation product not only can be rich in target milk flavor substances, but also can synergistically contain various flavor compounds such as esters, alcohols and aldehydes, and the richness of the flavor substances is improved; the anhydrous cream and the whey powder are prepared into the fermentation substrate, so that a comprehensive and balanced growth environment is provided for the German-type lactobacillus bulgaricus subspecies, and the fermentation efficiency is improved.

The invention is suitable for the technical field of biological medicine, and provides an active composition for relieving fatigue and application of the active composition. The composition is prepared from a silkworm chrysalis-derived mitochondrial repair peptide, a chickpea-derived neuromodulation peptide, a haematococcus pluvialis-derived phagocytosis peptide, a phosphatidylcholine-lipoic acid conjugate, a whey protein-chitosan copolymer and an ergothioneine-selenium compound in a synergistic manner. Through the synergistic effect of four mechanisms of energy metabolism activation, endocrine balance, mitochondrial autophagy repair and oxidative stress removal, physiological fatigue and stress fatigue are effectively and synchronously relieved. After targeted modification, the utilization rate of macamides is remarkably increased, and the composition can remarkably prolong exercise tolerance, reduce cortisol level, promote GABA synthesis, recover mitochondrial function and greatly shorten fatigue recovery time.

The composition and / or combinations includes diosgenin and at least one other agent selected from vitamin D and α-lactalbumin and its use in the regularization of the menstrual cycle and in the reduction of ovarian cysts, in particular in women affected with polycystic ovarian syndrome (PCOS) phenotype D.

The invention discloses a recombinant human lactalbumin plant expression cassette and construction and application thereof, and belongs to the technical field of genetic engineering and biological manufacturing. The invention aims to solve the problems of low heterologous expression efficiency and non-ideal expression quantity caused by mismatching of mammal-derived human lactalbumin genes and plant host codon use preferences. The method comprises the following steps: firstly obtaining an amino acid sequence of human lactalbumin, then optimizing a coding nucleotide sequence according to the codon use frequency of a target plant host species, and finally inserting the optimized gene into a linearized vector skeleton containing a plant specific promoter and a plant recognizable terminator through enzyme digestion connection or homologous recombination. The optimized gene is located downstream of the promoter and upstream of the terminator. The method provides support for high-efficiency expression of the recombinant human lactalbumin in plant host cells, and assists application of the recombinant human lactalbumin in food, nutritional health-care products or pharmaceutical preparations.

The invention discloses a step-by-step enzymolysis method for alpha-lactalbumin allergic epitopes and application, and belongs to the technical field of dairy product processing. Through step-by-step directional enzymolysis of flavourzyme and alpha-chymotrypsin, allergic epitopes in ALA are directionally cleared, and the antigenicity of ALA is remarkably reduced. The method is simple and convenient in process, mild in condition and suitable for large-scale production, and the obtained enzymolysis product can be used for low-sensitization infant formula milk powder and food for special medical purposes and has important application value and social benefits.

The invention discloses a postbiotic with a sleep promoting effect as well as a preparation method and application of the postbiotic, and provides a scheme for efficiently preparing the postbiotic for improving sleep at low cost. The preparation method of the fermentation culture medium comprises the following steps: mixing milk protein with a solvent to obtain milk protein liquid, and sequentially adding pepsase and mixed enzyme for enzymolysis to obtain zymolyte; the mixed enzyme is selected from at least one of trypsin or alkaline protease; the milk protein is selected from milk protein or casein or whey protein. According to the fermentation medium and the method for preparing the metagen by using the fermentation medium, the gamma-aminobutyric acid precursor substance sodium glutamate or glutamic acid does not need to be additionally added into the fermentation agent, and the prepared metagen has a good sleep improving effect and can be used for industrial production.

The application discloses a kind of alpha-lactalbumin multifunctional peptides with antioxidant, anticoagulant and tyrosinase inhibitory activity and application thereof, and belongs to the field of food, biopharmaceutical and cosmetic technology.The multifunctional peptide SEKLDQWLCEKL is screened, and the DPPH clearance rate and ABTS clearance rate of the multifunctional peptide SEKLDQWLCEKL are highest, which can reach 62.32% and 88.32% respectively, the clearance rate of hydroxyl radical is highest, which can reach 78.89%, PT and TT are respectively extended by 3.4 s and 4.7 s, and the inhibition rate of tyrosinase is highest, which can reach 89.07%, which overcomes the limitation of existing single functional peptide in application.

The invention provides a screening method of an alpha-lactalbumin detection antibody and application of the screening method in alpha-lactalbumin detection and dairy product process authenticity evaluation, and relates to the technical field of dairy product process detection. The screening method of the detection antibody for recognizing the lactalbumin comprises the following steps: taking animal serum immunized by alpha-lactalbumin, carrying out affinity purification to obtain candidate antibodies, and carrying out primary screening and final screening on the candidate antibodies to obtain the detection antibody. The detection antibody screened by the method is highly specific to alpha-lactalbumin, cross reaction of other proteins in a milk matrix can be avoided, and the reliability of actual detection is ensured. According to the invention, a screened detection antibody is also utilized to construct a'hot processing parameter-ELISA antigen recognition performance 'correlation model, and an efficient technical means is provided for rapid traceability of hot processing technologies such as milk pasteurization and UHT technology.

The invention provides a nutritional composition capable of improving bioavailability of stable amino acid and application of the nutritional composition. The invention firstly provides a composition which comprises alpha-lactalbumin, linoleic acid and prebiotics, and the mass ratio of the alpha-lactalbumin to the linoleic acid to the prebiotics is (0.25-0.6): 1: (0.1-0.96). The invention also provides a food containing the composition, and an application of the composition or the food in preparation of a product for improving the bioavailability of the stable amino acid.

The invention relates to the technical field of microbial preparations, and discloses a lactobacillus salivarius preparation, which comprises a core material and a wall material, the core material comprises a lactobacillus salivarius suspension and a protective agent solution, and the wall material is 1-3% by mass of a sodium alginate aqueous solution or 5-10% by mass of a whey protein aqueous solution. The protective agent solution comprises the following components in percentage by mass: 5-8% of glycerol, 10-15% of skim milk powder, 0.3-0.8% of sodium ascorbate and 6-10% of trehalose. By systematically screening and optimizing the components and proportion of the protective agent, an efficient protective system is constructed, the survival rate of thalli in the freeze-drying process and the stability of the preparation in long-term storage are remarkably improved, the preparation can quickly recover and maintain the healthy acidic environment of vagina, and the preparation has a good application prospect. Breeding of various common harmful bacteria is remarkably inhibited, and the specific effects of adjusting vagina micro-ecological balance and preventing and assisting in treating related gynecological inflammation are achieved.

To provide a combination chemotherapy exhibiting a large therapeutic effect on cancer, especially bladder cancer.SOLUTION: There is provided a first chemotherapeutic agent and a second chemotherapeutic agent for use in the treatment of cancer, wherein the second chemotherapeutic agent comprises a biologically active complex having anti-tumour activity, wherein the biologically active complex consists of a peptide of at least 10 amino acids comprising an alpha-helical structure and oleic acid or oleate in a ratio of at least 3 oleic acid or oleate molecules per peptide molecule. Provided are either a first chemotherapeutic agent or a second chemotherapeutic agent for use in cancer therapy, for use in conjunction with other chemotherapeutic agents, pharmaceutical compositions related thereto, and methods of treatment.SELECTED DRAWING: None

The invention discloses a nutritional composition for promoting absorption of amino acid and mineral substances and application, and belongs to the technical field of food. The nutritional composition provided by the invention comprises alpha-lactalbumin and breast milk oligosaccharide (especially 6 '-SL and / or 3-FL), and can help to promote absorption of amino acids and mineral substances by a body, especially help to promote transmembrane transport capability of intestinal cells of the body to amino acids and mineral substances, thereby improving absorption efficiency.

The subject of the application is a dietary supplement containing 2.5 - 7 wt.% indole-3-carbinol, 2 - 6 wt.% epigallocatechin gallate, 30 - 70 wt.% myo-inositol, 6 - 12.5 wt.% N-acetylcysteine, 2.5 - 7 wt.% quercetin, 5 - 8.5 wt.% Omega-3 acid, 5 - 8.5 wt.% alphalipoic acid, 2 - 6 wt.% vitamin C, 1 - 5 wt.% resveratrol, 0.01 - 0.2 wt.% vitamin D3, 0.5 - 3.5 wt.% vitamin E, 0.2 - 15 wt.% alpha-lactalbumin, 0.04 - 20 wt.% curcumin, 0.2 – 15% w / w D-chiroinositol, 0.2- 24% w / w inulin, a probiotic consisting of a mixture of strains of the Lactobacillus, Bifidobacterium and Saccharomyces genera for patients with reproductive system diseases supporting the reduction of estrogen levels and estrogen and progesterone receptors.

Synthetic nutritional composition comprising a phospholipid-enriched milk fraction and a milk serum protein concentrate (SPC) for use in the prevention of an influenza virus and / or the prevention of further development of a viral infection with influenza virus.

Adhesive formulations comprising bovine serum albumin, b-lactoglobulin, and / or a-lactalbumin can be applied and cured underwater to form a strong adhesive. Curing can be accomplished using heat or via a chemical denaturing component.

Synthetic nutritional composition comprising a phospholipid-enriched milk fraction and a milk serum protein concentrate (SPC) for use in the prevention of a viral infection with respiratory syncytial virus (RSV) and / or the prevention of further development of a viral infection with respiratory syncytial virus (RSV).

The invention discloses a high-performance tissue sealant as well as a preparation method and application thereof, and relates to the technical field of medical materials. The tissue sealant is prepared from the following raw materials: amino-containing protein, a multi-arm polyethylene glycol derivative and an optional multi-amino polymer, wherein the amino-containing protein comprises at least one of lactoferrin, phycocyanin, whey protein, beta-lactoglobulin, cytochrome C, recombinant collagen, recombinant fibronectin and alpha-lactalbumin. The sealant provided by the invention has the characteristics of rapid gelling, strong adhesion, high mechanical strength, low swelling, antibacterial property and the like, can realize efficient tissue wound sealing, hemostasis and repair in a wet complex environment, is simple and convenient to prepare, safe in material source and good in biocompatibility, is suitable for being used in various wound scenes, and has wide application prospects. Good application prospects are realized.