Method of modifying lactoalbumin by enzymatic method and its application
A milk protein modification technology, applied in the fields of application, dairy products, milk preparations, etc., can solve the problems of low concentration of protein solution, inability to realize industrialization, difficulties in industrialized production, shorten the process route, and solve food safety problems , the effect of increased selectivity
Examples
Embodiment 1
[0026] Fresh milk is separated by centrifuge to remove fat to obtain skim milk. Skim milk is ion-adjusted and heat-treated: use Ca in a ratio of 1-2:1-2:1 2+ 、K + and Na + The ion concentration of the solution is adjusted so that the pH reaches 7.0-7.5; the protein concentration in the solution is 3.1%, and the temperature is raised to 70-90°C through a heat exchanger, maintained for 5-15s, and then cooled to 45-65°C. Immobilized compound enzyme enzymatic hydrolysis: the enzymes are papain and trypsin, the ratio of the two enzymes is 4:6, the carrier is sodium alginate, the concentration of sodium alginate solution is 5%, the enzyme / carrier mass ratio is 1:25, Glutaraldehyde was added dropwise to make the concentration of glutaraldehyde in the solution 0.6%, and the reaction was stirred at 35° C. for 10 h to obtain the immobilized complex enzyme. The enzymatic hydrolysis conditions are: the amount of enzyme is 1‰ of the milk protein mass, 55°C for 2.5h, α s -The modificati...
Embodiment 2
[0028] Fresh milk is separated by centrifuge to remove fat to obtain skim milk. Add desalted whey powder to skim milk, the addition amount is 5% mass / volume ratio, after ion adjustment and heat treatment: use Ca in the ratio of 1~2:1~2:1 2+ 、K + and Na + The solution adjusts the ion concentration to make the pH reach 7.0-7.5; the protein concentration in the solution is about 3.7%, and the temperature is raised to 70-90°C through a heat exchanger, maintained for 5-15s, and then cooled to 45-65°C. Immobilized compound enzyme enzymatic hydrolysis: the enzymes are papain and pepsin, the ratio of the two enzymes is 5:5, the carrier is chitosan, the concentration of chitosan solution is 3.5%, and chitosan should be 1%-2% Dissolve the acetic acid solution, adjust the pH to 7.5 with 6mol / L NaOH, drain and wash with pH 7.5 phosphate buffer, then add the enzyme solution, stir and adsorb for 30-40min, the enzyme / carrier mass ratio is 1:50, Glutaraldehyde was added dropwise so that th...
Embodiment 3
[0030] Fresh milk is separated by centrifuge to remove fat to obtain skim milk. Add desalted whey powder to skim milk, the addition amount is 10% mass / volume ratio, after ion adjustment and heat treatment: use Ca in the ratio of 1~2:1~2:1 2+ 、K + and Na + The solution adjusts the ion concentration to make the pH reach 7.0-7.5; the protein concentration in the solution is about 4.4%, and the temperature is raised to 70-90°C through a heat exchanger, maintained for 5-15s, and then cooled to 45-65°C. Immobilized composite enzyme enzymolysis: the enzymes are papain and bromelain, the ratio of the two enzymes is 6:4, the carrier is sodium alginate / chitosan, and the concentration of sodium alginate / chitosan solution is 2% (alginic acid The sodium concentration is 1%, the chitosan concentration is 1%), the enzyme / carrier mass ratio is 1: 10, and glutaraldehyde is added dropwise so that the glutaraldehyde concentration in the solution is 0.3%, and stirred and reacted at 10°C for 5h ...
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Description
Claims
Application Information
- IPC
- A23C9/12; A23C9/13
- Inventors
- 夏文水; 陈洁