28results about How to "Simple extraction method" patented technology

The invention discloses a human body detection and face recognition system and method, and belongs to the technical field of video stream real-time monitoring and face recognition, and the method comprises the steps: 1, carrying out the motion detection of an obtained video stream transmitted by a camera; 2, executing human body detection according to a motion area obtained by motion detection; step 3, executing face feature extraction in a local face database; step 4, executing multi-image fusion in the video stream; step 5, performing face detection on the detected human body area; step 6, performing face recognition after face detection is performed; the face feature extraction and recognition method is further optimized, so that the detection and recognition precision and the real-time performance of the system in practical application and the adaptive capacity in a complex scene are remarkably improved, and the robustness is good.

The invention discloses an information query method and device, electronic equipment and a readable storage medium. The method comprises the steps of obtaining a query word input by a target object; determining a plurality of target keywords corresponding to the query word; for each target keyword, determining a plurality of target word frequencies respectively corresponding to each target keyword, determining an inverse document frequency respectively corresponding to each target keyword, and determining a plurality of word distance factors respectively corresponding to each target keyword; according to a plurality of target word frequencies respectively corresponding to each target keyword, an inverse document frequency respectively corresponding to each target keyword and a plurality of word distance factors respectively corresponding to each target keyword, sub-query results respectively corresponding to the plurality of target keywords are determined; and determining a target query result corresponding to the query word according to the sub-query results corresponding to the plurality of target keywords. The technical problem that corresponding information is difficult to accurately query in related technologies is solved.

The invention discloses a lightweight image super-resolution reconstruction system and method for underground mobile equipment, and belongs to the technical field of image reconstruction. Comprising a shallow feature extraction module, a deep feature extraction module, a feature aggregation module and an image reconstruction module. The deep feature extraction module comprises a high-frequency enhanced distillation block, and the high-frequency enhanced distillation block comprises a feature refining module, a multi-scale information distillation module and an information fusion module; and the feature refining module comprises three high-frequency enhanced residual blocks which are connected in series. According to the method, an information distillation architecture is adopted, a two-way branch structure is adopted in a feature extraction trunk, channel segmentation is carried out on input features, the calculation amount is reduced, the capturing capacity of a network on detail information is improved by utilizing global feature extraction and high-frequency enhancement branches, and deep information is efficiently extracted; and adopting a multi-scale information distillation method to perform multi-scale high-frequency information distillation on the features after feature extraction trunk refinement, and fusing the distilled multi-scale high-frequency information by using a mixed feature enhancement block.

The invention discloses spirulina alpha-glucan containing soluble dietary fibers and having immunomodulatory activity and a preparation method thereof, spirulina is subjected to continuous illumination culture under nutrient salt deficiency or low temperature or combined regulation and control of low temperature and nutrient salt deficiency; a large amount of alpha-glucan which contains more soluble dietary fibers and has immunoregulatory activity is accumulated in the spirulina, and the spirulina alpha-glucan is prepared by adopting a method of hot water extraction, trichloroacetic acid deproteinization and ethanol precipitation. The prepared spirulina alpha-glucan contains 10%-26% of dietary fibers, the content of the dietary fibers is 9-27 times higher than the content of the dietary fibers of maltodextrin, the spirulina alpha-glucan can induce secretion of NO, TNF-alpha and IL-6 of macrophages RAW264.7, the activation index of the NO reaches 1.5-3.6, the activation index of the TNF-alpha reaches 2.0-4.1, the activation index of the IL-6 reaches 1.6-3.0, and the spirulina alpha-glucan has a remarkable immunoregulation effect. Technical support and product cases are provided for development of spirulina glucan type high-added-value functional products.

The present application relates to the field of analytical chemistry, and provide a kind of method for detecting complex matrix of agricultural products in guanidine morpholino salt hydrochloride.The extraction solvent of the present application uses acetonitrile+acid+aqueous solution, different acid is selected according to the water content of sample, trifluoroacetic acid is used for high water content sample, and formic acid is used for low water content sample;In purification, pH value is adjusted according to the oil content of sample, ammonia is used to adjust pH to 8.5 for high oil content sample, and pH value can not be adjusted for low oil content sample.In the method, NaCl is not added to make organic phase and aqueous phase in extraction liquid stratified, through the way of extracting three times repeatedly, the recovery rate of the method is guaranteed;And using ultra-high phase liquid chromatography triple quadrupole mass spectrometry technology, guanidine morpholino salt hydrochloride in complex matrix of agricultural products is successfully detected, the extraction method is fast and simple, the quantitative limit of guanidine morpholino salt hydrochloride in tea is 0.1mg / kg, and the quantitative limit in other matrix is 0.05mg / kg, which is suitable for routine analysis, and can meet the quantitative detection requirements of guanidine morpholino salt hydrochloride in complex matrix of agricultural products.

This invention provides a dressing containing Ligusticum striatum microspheres and its preparation method, to solve the problem of safe and precise application of Ligusticum striatum extract or Ligustrazine in difficult-to-heal wounds; the dressing containing Ligusticum striatum microspheres and its preparation method, wherein the dressing is composed of Ligusticum striatum microspheres loaded with pHEMA hydrogel as the main body, wherein the Ligusticum striatum microspheres are formed by coating Ligusticum striatum extract or Ligustrazine with PLGA, and the pHEMA hydrogel partially encapsulates the Ligusticum striatum microspheres.

The invention provides an extraction method of molybdenum trioxide, and belongs to the technical field of extraction metallurgy. The extraction method of molybdenum trioxide provided by the invention comprises the following steps: carrying out vacuum melting on a molybdenum-containing material to obtain a sublimate, and cooling the sublimate to obtain molybdenum trioxide, the molybdenum content in the molybdenum-containing material is 37.42 to 46.31 wt%, and the oxygen content in the molybdenum-containing material is 30.74 to 38.13 wt%; the temperature of vacuum melting ranges from 880 DEG C to 1200 DEG C, and the vacuum degree ranges from 0.47 Pa to 10 Pa; the cooling rate of the cooling is 5-10 DEG C / min. According to the method, the molybdenum-containing material is subjected to vacuum melting, molybdate in the molybdenum-containing material is decomposed to obtain molybdenum trioxide, original molybdenum trioxide of the molybdenum-containing material is sublimated under the high-temperature condition, high-purity molybdenum trioxide is obtained through cooling, and the extraction efficiency of molybdenum trioxide is effectively improved; and the extraction method is simple, the low-grade molybdenum-containing material is used as the raw material, the extraction cost is lower, and no pollution is caused to the environment.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The invention provides gardenia polysaccharide, a preparation method thereof and application of the gardenia polysaccharide in preparation of medicines for treating pulmonary fibrosis, and relates to the technical field of traditional Chinese medicine extracts and biological medicines. The gardenia polysaccharide GP121 with a new polysaccharide structure is extracted from gardenia, and the gardenia polysaccharide GP121 provided by the invention is simple in extraction method, high in polysaccharide extraction rate and low in production cost. The invention further researches the anti-pulmonary fibrosis biological activity of the gardenia polysaccharide GP121 on the basis of the cellular level, and the research result shows that the gardenia polysaccharide GP121 provided by the invention has the effect of delaying the MRC-5 cell fibrosis process caused by TGF-beta1; the phosphorylation of the Smad3 protein is inhibited, so that the expression of alpha-SMA, CollagenI and FN protein is reduced, and the fibrosis process is delayed. The gardenia polysaccharide GP121 provided by the invention can be used as a potential therapeutic drug for treating pulmonary fibrosis, and a new application of the gardenia polysaccharide in the aspect of treating pulmonary fibrosis is provided.

The invention provides a plant glycosphingolipid extraction method, which comprises: taking a plant material, grinding liquid nitrogen, adding a hot ethanol solution (containing beta-mercaptoethanol), carrying out oscillation extraction at a high temperature, carrying out high-temperature high-speed centrifugation, taking the supernatant, carrying out low-temperature precipitation, and carrying out high-speed centrifugation to obtain a crude extract; washing the crude extract with acetone and diethyl ether, removing the organic solvent through high-speed centrifugation, adding a dissolving solution (tetrahydrofuran, methanol and water), carrying out vortex uniform mixing, carrying out high-temperature dissolution, filtering, and carrying out vacuum centrifugal drying on the filtrate to obtain a crude product; adding n-butyl alcohol and water into the crude product, dissolving, precipitating and centrifuging at high temperature, taking an upper layer n-butyl alcohol phase, and performing vacuum centrifugal drying to obtain a target product. The extraction method provided by the invention has the advantages of high extraction efficiency and time saving, and more importantly, small-weight plant material extraction can be effectively realized by virtue of simple equipment, and the sample size required by mass spectrum identification of GIPCs composition and ion chromatography identification of GIPCs glycosyl composition can be obtained.

The invention belongs to the technical field of compound traditional Chinese medicine preparation optimization algorithms and preparation optimization, and relates to a traditional Chinese medicine preparation optimization method based on a hemorheology mechanism and a preparation. Particularly relates to an optimization method of an antithrombotic yang-tonifying and recuperating decoction preparation and the preparation. According to the preparation optimization method, an intestinal flora regulator substance group and an anti-thrombus component substance group of medicinal material chemical components of the Yang tonifying and also five decoction and corresponding substance clusters of products obtained by different extraction methods are constructed, a better Yang tonifying and also five decoction extraction method is determined on the basis of intersection comparison of different substance groups, and then optimization is performed. The method can effectively guide the optimization of the Yang-tonifying and Yang-restoring decoction preparation. The Yang-tonifying Huanwu decoction preparation is prepared from a water decoction and alcohol precipitation extracting solution of raw radix astragali, radix paeoniae rubra and lumbricus and a 70% ethanol reflux extracting solution of the other four medicinal materials. The extracted components have the most intersection substances with the intestinal flora regulator substance group and the antithrombotic component substance group and the most intersection substances with high content, and have higher anticoagulant and antithrombotic effects.

The present invention discloses a method for extracting lithium from lepidolite, comprising: crushing the lepidolite and placing it in an aluminum salt solution for pressure leaching, filtering to obtain a first filtrate and a filter residue; removing iron from the first filtrate using an iron removal agent, filtering to obtain a second filtrate and iron-containing slag; removing fluorine and aluminum from the second filtrate, filtering to obtain a third filtrate and cryolite; precipitating lithium from the third filtrate, filtering to obtain lithium carbonate. The present invention is based on the coordination leaching of lithium from lepidolite by an aluminum salt system, achieving one-step leaching of lepidolite, the extraction method has a high yield, and the lithium carbonate finally obtained has a high purity; while extracting lithium carbonate, byproducts such as goethite and cryolite with high purity can be obtained to avoid waste. The extraction method of the present invention is simple, and the reagents used are all commercially available common reagents, with low cost and good lithium extraction effect, and is convenient for use in industrial production.

This disclosure relates to a method for extracting β-nicotinamide mononucleotide from microalgae and the microalgae extract. The method includes: mixing microalgae raw material with an extractant for extraction; wherein the microalgae raw material is selected from one or more of Chlorella, Spirulina, and Haematococcus pluvialis; the extractant comprises 0.05–1.5 wt% of a nonionic surfactant, 0.5–1.0 wt% of an organic acid, 20–40 wt% of an alcohol, and the balance being a solvent. Microalgae raw materials are easy to cultivate, grow rapidly, have high protein content, and high β-nicotinamide mononucleotide content; the extraction method disclosed herein is simple, has a high extraction rate, and is low in cost.

The present invention discloses seafood mushroom sweet peptides and an extraction method and application thereof, and belongs to the technical field of protein active peptides. The amino acid sequence of seafood mushroom sweet peptides is: Glu‑Gly‑Asn‑Pro‑Ala‑His‑Gln‑Lys, Gln‑Glu‑Gln‑Glu‑Ala‑Lys‑Lys‑Arg or Ala‑Gly‑Leu‑Glu‑Leu‑Leu‑Lys. The sweet peptides are all extracted from seafood mushrooms. The sweet peptides are small molecular weight peptides and are easily absorbed by the human body. The sensory test results show that the extracted sweet peptides have good sweetness and richness, can be used instead of sucrose, and have great application significance in the food field.

The invention provides a method for automatically extracting polybrominated organic pollutants in a bottom mud sample, which comprises the following steps: step 1, collecting the bottom mud sample, wrapping the bottom mud sample with an aluminum foil, and performing low-temperature cryopreservation; 2, freeze-drying the sample by using a freeze-drying machine, and then sieving by using a metal sieve to remove impurities; step 3, performing parameter and process setting on an automatic sample pretreatment system built in a laboratory; 4, mixing the pretreated bottom mud with diatomite, filling the mixture into a stainless steel extraction pool, placing a filter membrane at the bottom of the pool body, placing the pool body into a sample extraction position of an accelerated solvent extraction instrument, and extracting a sample to obtain a bottom mud extracting solution; step 5, each time one sample is extracted, the obtained extracting solution is automatically transferred to a full-automatic gel purification quantitative concentration system instrument by a mechanical arm to be concentrated and dissolved, and then is transferred and submitted to a gel permeation chromatography by a sample injection needle of the instrument; step 6, concentrating the extracting solution, and eluting and purifying by using gel permeation chromatography; and 7, introducing the concentrated solution purified by the gel permeation chromatography into a concentrator through an internal pipeline, concentrating in two modes of pressure reduction and heating, and finally transferring the concentrated solution into a brown small sample injection bottle through a sample injection needle to obtain an extracting solution of the sample. The invention aims to provide a method for efficiently and quickly extracting brominated organic pollutants in bottom mud, manual operation is less, the sample pretreatment time is shortened, the extraction efficiency is improved, and convenience is provided for subsequent compound analysis and identification.

The invention discloses an application of sphingomonas extracellular polysaccharide in treatment of glucose and lipid metabolism disorder. The application range of the sphingomonas exopolysaccharide is expanded, the source is natural and safe, and a new thought is provided for prevention and treatment of diseases.

The application discloses a preparation method of trans-cuparemic acid, which comprises the following steps: step one, crushing and mixing; step two, extracting by heating and refluxing with ethyl acetate; step three, secondary extraction; step four, reducing pressure concentration; step five, elution treatment; and step six, concentration and recrystallization; wherein in the step one, cupressaceae plants are dried and crushed, and then screened; subsequently, the screened cupressaceae plant powder is uniformly mixed with equal amount of calcium carbonate powder to obtain mixed powder, which is prepared for use; in the step two, appropriate mixed powder obtained in the step one is weighed, and a certain amount of ethyl acetate is added to obtain a mixture; subsequently, the obtained mixture is boiled by heating for 2 hours, and a first extraction liquid is obtained by filtering; the application can effectively avoid pigment interference, and only one column chromatography operation is needed in the separation process; the obtained trans-cuparemic acid has large yield and high purity, and can be produced on a large scale.

The invention provides a beta-carboline alkaloid coumaroyl glucoside compound, an extraction method and application thereof, and a monoamine oxidase inhibitor. The beta-carboline alkaloid coumaroyl glucoside compound provided by the invention has a structure as shown in a formula 1 or a formula 2, is novel in structure, has good monoamine oxidase inhibitory activity, can slow down monoamine neurotransmitter consumption in the brain, slow down oxidative damage and realize treatment of neurodegenerative diseases, and has good application prospects. Further, the compound can be used for preparing a monoamine oxidase inhibitor or preparing a medicine for resisting neurodegenerative diseases, can also be used for developing health-care food, and is wide in application prospect. # imgabs0 #

The invention relates to a trichosanthes kirilowii maxim alkaloid extract and an extraction method and application thereof, and belongs to the technical field of trichosanthes kirilowii maxim extract preparation. The extraction method comprises the following steps: performing ultrasonic extraction on trichosanthes peel powder for multiple times by using a sulfuric acid solution, combining extracting solutions obtained by multiple times of extraction, performing primary concentration on the combined extracting solutions, then adjusting the pH value of the extracting solutions obtained by primary concentration to a target pH value, performing extraction for multiple times by using n-butyl alcohol, combining the extracting solutions obtained by multiple times of extraction, performing secondary concentration to obtain paste, and performing spray drying to obtain the trichosanthes peel extract. The trichosanthes kirilowii maxim alkaloid extract is obtained. The content of alkaloid in the trichosanthes kirilowii maxim alkaloid extract is 60-70%, and the trichosanthes kirilowii maxim alkaloid extract contains 20 kinds of alkaloid, has a good anti-atherosclerosis pharmacological action, and can remarkably improve liver lipid deposition, liver lipid metabolism disorder and abnormal blood lipid level of atherosclerosis mice.

The invention discloses degreased evening primrose seed protein hydrolysate, a preparation method thereof and application of the degreased evening primrose seed protein hydrolysate in prevention and treatment of diabetic retinopathy, and belongs to the technical field of biological medicine. The degreased evening primrose seed protein hydrolysate is obtained by hydrolyzing degreased evening primrose seed protein with compound protease, and the degreased evening primrose seed protein is prepared by alkali extraction and acid precipitation of degreased evening primrose seeds. An extraction method of the degreased evening primrose seed protein is optimized, an enzyme most suitable for hydrolyzing the degreased evening primrose seed protein and an optimal hydrolysis condition are found through experiments, and the experiments prove that the degreased evening primrose seed protein hydrolysate prepared by the invention has a remarkable intervention effect on high-glucose-induced zebra fish retinal vascular injury, and can be used for preparing a medicine for treating hyperglycemia-induced zebra fish retinal vascular injury. The invention provides a new data reference and action mechanism for prevention and treatment of diabetic retinopathy.

The invention provides an earthworm protein extract as well as an extraction method and application thereof. The earthworm protein extract can be applied to agricultural production. The extraction method comprises the following steps: preparing cleaned earthworms into earthworm homogenate; adding water into the earthworm homogenate, and carrying out solid-liquid separation to obtain an extracting solution containing earthworm protein; then protease is added for enzymolysis; and performing purification treatment on the extracting solution after enzymolysis to obtain the earthworm protein extract. The extraction method of the earthworm protein extract is simple, extraction parameters are controllable, and the extracted earthworm protein is high in extraction rate and purity. The earthworm protein extract is rich in eighteen amino acids, various nucleotides, FA proteins and other components, can provide essential raw materials for plant metabolism, is beneficial to plant tissues to coordinate cell division and differentiation activities, can effectively improve the yield, quality and resistance of crops, and has the effects of improving the soil structure, improving the soil fertility and the like.

The invention provides a beta-carboline alkaloid derivative, an extraction method and application thereof, and a monoamine oxidase inhibitor. The beta-carboline alkaloid derivative provided by the invention has a structure as shown in formula 1, is novel in structure, has good monoamine oxidase inhibitory activity, can slow down consumption of monoamine neurotransmitters in the brain, slow down oxidative damage and realize treatment of neurodegenerative diseases, and has good application prospects. Further, the compound can be used for preparing a monoamine oxidase inhibitor or preparing a medicine for resisting neurodegenerative diseases, can also be used for developing health-care food, and is wide in application prospect. # imgabs0 #

The invention provides a method for characterizing the performance of an inhalation vaccine, which comprises the following steps of: quantitatively and jointly using an NGI cascade impactor, a polymerase chain reaction real-time fluorescent quantitative PCR (polymerase chain reaction) (Quantitative Real-time PCR, QPCR) and a droplet digital PCR (drop digital PCR, ddPCR), and establishing an integrity performance characterization method aiming at the inhalation vaccine; a plurality of influence factors of the NGI cascade impactor in characterization of the inhaled vaccine are deeply researched, and a detection environment with stable results and high repeatability is obtained; meanwhile, the optimal characterization sample dosage is determined based on multiple repeated experiments; the invention innovatively provides the combination of NGI, QPCR and ddPCR, and the method has the advantages of accurate detection and short detection time, can meet the detection of large-batch samples, and ensures the large-scale application in the industry.

The invention provides an okra lubricant as well as a preparation method and application thereof. The abelmoschus esculentus lubricant is obtained by mixing abelmoschus esculentus mucus powder with a NaCl solution, and the abelmoschus esculentus mucus powder is prepared by a soaking method and contains components such as a water-soluble polysaccharide compound, vegetable protein and mineral substances. And mixing the okra hydrogel with acrylamide, N, N-methylene bisacrylamide and a photoinitiator, stirring, carrying out ultrasonic degassing, and carrying out ultraviolet curing to obtain the okra hydrogel lubricant. The abelmoschus esculentus lubricant and the abelmoschus esculentus hydrogel lubricant prepared by the preparation method disclosed by the invention have good stability, adsorbability, lubricity and wear resistance, and meanwhile, good biological safety is ensured. And the preparation method is simple, the cost is low, and the lubricating grease has the characteristic of super lubrication (friction coefficient [mu] lt: 0.01) under high loading, and can meet the requirement on low friction in practical application.

The invention belongs to the technical field of natural product extraction, and particularly relates to a method for extracting gastrodia elata total polyphenol active substances in food and cosmetics. According to the method, a eutectic solvent is used as an extraction solvent, and ultrasonic extraction is combined; a hydrogen bond acceptor of the eutectic solvent comprises polyethylene glycol-200, polyethylene glycol-400 and Tween 80, and a hydrogen bond donor comprises ethylene glycol, 1, 2-propylene glycol, glucose, glycerol and xylitol. According to the method, the eutectic solvent is used as an extraction solvent, and ultrasonic extraction is combined, so that the extraction efficiency of the total polyphenol substances of the gastrodia elata is high and can reach 33.2 mg / g at most; the extraction method disclosed by the invention is simple, short in extraction time and practical, and has a very good development prospect in the fields of medicines, foods, cosmetics and the like.

The invention discloses a rape pollen mask and a preparation method, and belongs to the technical field of mask preparation. The mask ingredients include glycerin, sodium hyaluronate, carbomer, triethanolamine, rape bee pollen extract, 1,2-propylene glycol, vitamin B5, methyl paraben, hydrogenated castor oil and deionized water, and the preparation method of the rape pollen mask includes the following steps: 1) preparing phase A: weighing the corresponding mass of deionized water, adding glycerin, stirring until the water and glycerin are completely dissolved, adding sodium hyaluronate to completely dissolve it, adding a certain mass of carbomer, and stirring until uniform; 2) preparing phase B: weighing a certain amount of 1,2-propylene glycol, adding methyl paraben thereto, and then placing it in a 60°C water bath and stirring until completely dissolved; 3) after mixing phase A and phase B evenly, hydrogenated castor oil, rape bee pollen flavonoid extract and vitamin B5 are added thereto one by one, and finally adjusted to a suitable pH with triethanolamine, and continuously stirred to eliminate bubbles.

The present invention relates to the field of medical technology, and provides a method for preparing a segmented polysaccharide of Sargassum fusiformis and an application thereof in preparing an anti-RSV drug. The segmented polysaccharide of Sargassum fusiformis is prepared by the following steps: (1) preparing crude Sargassum fusiformis polysaccharide; (2) preparing an intermediate product; (3) preparing Sargassum fusiformis polysaccharide; (4) dissolving the Sargassum fusiformis polysaccharide in a solvent, passing the supernatant through different molecular retention plates, and then freeze-drying to obtain the segmented polysaccharide of Sargassum fusiformis with a corresponding molecular weight. The segmented polysaccharide of Sargassum fusiformis prepared by the present invention has a significant inhibitory effect on RSV, and its drug inhibition index (TI) can reach more than 4000.