74results about How to "Simple extraction method" patented technology

The invention discloses an extraction method and application of fungus source exosome-like nano vesicles. The extraction method comprises the following steps: preparing a fungus filtrate by a hot water method, carrying out centrifugation and filtration purification on the fungus filtrate, and then carrying out ultrafiltration interception to obtain the fungus-derived exosome-like nano-vesicles. The extracted fungus-derived exosome-like nano-vesicles have a lipid bilayer structure, are uniform in particle size distribution, are rich in lipid, nucleic acid and protein, are non-toxic, and have a remarkable anti-inflammatory effect; the extraction method provided by the invention is suitable for extracting exosome-like nano vesicles in various fungal samples, and has high yield and high activity.

The invention discloses a human body detection and face recognition system and method, and belongs to the technical field of video stream real-time monitoring and face recognition, and the method comprises the steps: 1, carrying out the motion detection of an obtained video stream transmitted by a camera; 2, executing human body detection according to a motion area obtained by motion detection; step 3, executing face feature extraction in a local face database; step 4, executing multi-image fusion in the video stream; step 5, performing face detection on the detected human body area; step 6, performing face recognition after face detection is performed; the face feature extraction and recognition method is further optimized, so that the detection and recognition precision and the real-time performance of the system in practical application and the adaptive capacity in a complex scene are remarkably improved, and the robustness is good.

The invention relates to the field of biological fermentation, in particular to a method for fermenting cacumen biotae with compound bacteria and application of the cacumen biotae, and the compound bacteria comprise lactococcus lactis and pichia pastoris. The method comprises the following steps: (1) pretreating cacumen biotae; (2) performing enzyme treatment on the cacumen biotae; (3) fermenting the cacumen biotae; and (4) treating fermentation liquor. The invention has the following beneficial effects: (1) enzymolysis parameters are optimized, fermentation parameters are optimized, a fermentation liquor extraction method is optimized, and a better fermentation method is obtained; (2) the Chinese arborvitae twigs are fermented, so that compared with fermentation of various traditional Chinese medicine raw materials in the prior art, the components are simplified, and the method is more convenient; and (3) a mouse experiment proves that more flavones and a better anti-hair loss effect can be obtained by using the optimized fermentation method, and the application of the cacumen biotae is more facilitated.

The invention discloses an information query method and device, electronic equipment and a readable storage medium. The method comprises the steps of obtaining a query word input by a target object; determining a plurality of target keywords corresponding to the query word; for each target keyword, determining a plurality of target word frequencies respectively corresponding to each target keyword, determining an inverse document frequency respectively corresponding to each target keyword, and determining a plurality of word distance factors respectively corresponding to each target keyword; according to a plurality of target word frequencies respectively corresponding to each target keyword, an inverse document frequency respectively corresponding to each target keyword and a plurality of word distance factors respectively corresponding to each target keyword, sub-query results respectively corresponding to the plurality of target keywords are determined; and determining a target query result corresponding to the query word according to the sub-query results corresponding to the plurality of target keywords. The technical problem that corresponding information is difficult to accurately query in related technologies is solved.

The invention discloses a method for simply extracting and accurately determining and quantifying micro-nano plastic in biological fluid. The method comprises the following steps: heating and digesting the biological fluid by using a strong oxidant; after digestion, adding a moistening solution for ultrasonic treatment, collecting the treated solution, and detecting the particle size of particles in part of the mixed solution; and filtering the residual mixed solution to separate out the micro-plastics. Meanwhile, collecting a filtrate containing the nano plastic, and directly transferring the filtrate into a sample boat to be heated and concentrated until the filtrate is dry; and a thermal cracking / thermal desorption-gas chromatograph-mass spectrometer is used for detecting the types and contents of the medium-micro plastic and the nano plastic. According to the method, the micro-nano plastic in different biological fluids can be simply extracted, accurate qualitation and quantification can be realized, and the technical bottlenecks that the extraction of the micro-nano plastic in the biological fluids is tedious and the particle size of the micro-nano plastic particles and accurate qualitation and quantification cannot be considered at the same time at present are broken; and an available technical method is provided for comprehensively and deeply analyzing the in-vivo health risk of the micro-nano plastic.

The invention relates to a composition for a pesticide, an insecticide and application of the insecticide, the composition comprises emamectin benzoate and a bamboo vinegar diluent, and the bamboo vinegar diluent is obtained by diluting bamboo vinegar with water. The insecticide comprises the composition for the pesticide, and the insecticide is applied to prevention and treatment of lepidoptera pests, mites, coleoptera pests and homoptera pests, especially to prevention and treatment of plutella xylostella. In the composition disclosed by the invention, the bamboo vinegar diluent is used as a synergist to improve the pesticide effect of the emamectin benzoate, so that the pesticide containing the composition can improve the effect of preventing and treating lepidoptera pests, mites, coleoptera pests and homoptera pests, particularly has a remarkable effect of preventing and treating plutella xylostella, and further has a remarkable effect of preventing and treating the emamectin benzoate. According to the insecticide, the dosage of the emamectin benzoate can be reduced, the drug resistance of pests can be overcome and delayed, and meanwhile, the risk levels of environmental pollution, pesticide residues and the like can be reduced.

The invention provides a preparation method of a biological functional agent containing bioactive components, which comprises the following steps: dispersing the bioactive components in water; adding the zeolite-loaded titanium dioxide composite material, absolute ethyl alcohol, isopropyl triisostearoyl titanate and polyvinylpyrrolidone into the mixed solution, stirring at the temperature of 65-75 DEG C to obtain a mixed solution containing bioactive components, stirring for 80-100 minutes, and centrifugally drying to obtain the composite material. According to the preparation method of the biological functional agent containing the bioactive components, the loading capacity of the bioactive components on the carrier is improved, the stability of the bioactive components is improved, and the slow release effect is enhanced, so that the preparation efficiency and performance of the biological functional agent are improved; wide prospects are provided for application in the fields of medicines, cosmetics, environmental protection and the like.

The invention discloses a lightweight image super-resolution reconstruction system and method for underground mobile equipment, and belongs to the technical field of image reconstruction. Comprising a shallow feature extraction module, a deep feature extraction module, a feature aggregation module and an image reconstruction module. The deep feature extraction module comprises a high-frequency enhanced distillation block, and the high-frequency enhanced distillation block comprises a feature refining module, a multi-scale information distillation module and an information fusion module; and the feature refining module comprises three high-frequency enhanced residual blocks which are connected in series. According to the method, an information distillation architecture is adopted, a two-way branch structure is adopted in a feature extraction trunk, channel segmentation is carried out on input features, the calculation amount is reduced, the capturing capacity of a network on detail information is improved by utilizing global feature extraction and high-frequency enhancement branches, and deep information is efficiently extracted; and adopting a multi-scale information distillation method to perform multi-scale high-frequency information distillation on the features after feature extraction trunk refinement, and fusing the distilled multi-scale high-frequency information by using a mixed feature enhancement block.

The invention discloses spirulina alpha-glucan containing soluble dietary fibers and having immunomodulatory activity and a preparation method thereof, spirulina is subjected to continuous illumination culture under nutrient salt deficiency or low temperature or combined regulation and control of low temperature and nutrient salt deficiency; a large amount of alpha-glucan which contains more soluble dietary fibers and has immunoregulatory activity is accumulated in the spirulina, and the spirulina alpha-glucan is prepared by adopting a method of hot water extraction, trichloroacetic acid deproteinization and ethanol precipitation. The prepared spirulina alpha-glucan contains 10%-26% of dietary fibers, the content of the dietary fibers is 9-27 times higher than the content of the dietary fibers of maltodextrin, the spirulina alpha-glucan can induce secretion of NO, TNF-alpha and IL-6 of macrophages RAW264.7, the activation index of the NO reaches 1.5-3.6, the activation index of the TNF-alpha reaches 2.0-4.1, the activation index of the IL-6 reaches 1.6-3.0, and the spirulina alpha-glucan has a remarkable immunoregulation effect. Technical support and product cases are provided for development of spirulina glucan type high-added-value functional products.

The invention provides a robust watermarking method for authenticating authenticity of a 3D printing model, which comprises a registration stage and a verification stage, and is characterized in that robust watermarking design and embedding of the 3D printing model are completed in the registration stage, and authentication of the 3D printing model is completed in the verification stage; according to the method, authentication information is converted into a binary bit string, and embedded binary bit watermark information is formed with the assistance of a verification data string and a positioning flag bit; selecting an area in which the watermark is embedded in the model; the joint structure in the printing process serves as a watermark carrier, the moving direction of a printer nozzle in an embedding area is modified according to watermark information, and embedding of the watermark information is achieved; and the watermark position in the model entity is positioned through the target detection network, and watermark information is extracted to judge the authenticity of the model. According to the method, the integrity of a model entity can be ensured in the process of authenticating the authenticity of the 3D printing model, the watermark is simple to embed and extract, the detection result is excellent, the anti-attack capability is high, and the robustness is high; and authentication cost is low and practicality is high.

The invention relates to application of wheat straw wood vinegar as a prothioconazole synergist, and belongs to the technical field of pesticide compounding. The invention provides application of wheat straw wood vinegar as a bactericidal synergist of a bactericide in prevention and treatment of wheat scab. According to the invention, the wheat straw pyroligneous liquor is used as a bactericidal synergist of prothioconazole, so that the pesticide effect of prothioconazole can be remarkably improved, the dosage of prothioconazole can be greatly reduced under the same control effect, the pesticide resistance of pests is overcome or delayed, and the pesticide effect of prothioconazole is improved. The composition prepared by mixing the wheat straw pyroligneous liquor with prothioconazole can reduce DON toxin in wheat grains in the harvesting period and has a remarkable effect on preventing and treating wheat scab, the wheat straw pyroligneous liquor is wide in raw material source, the extraction method is simple and convenient, and the wheat straw pyroligneous liquor has the advantages of being safe, environmentally friendly and capable of reducing environmental pollution. And the advantages of difficult generation of cross resistance with chemical pesticides and easy degradation of plant-derived synergists are achieved.

The present application relates to the field of analytical chemistry, and provide a kind of method for detecting complex matrix of agricultural products in guanidine morpholino salt hydrochloride.The extraction solvent of the present application uses acetonitrile+acid+aqueous solution, different acid is selected according to the water content of sample, trifluoroacetic acid is used for high water content sample, and formic acid is used for low water content sample;In purification, pH value is adjusted according to the oil content of sample, ammonia is used to adjust pH to 8.5 for high oil content sample, and pH value can not be adjusted for low oil content sample.In the method, NaCl is not added to make organic phase and aqueous phase in extraction liquid stratified, through the way of extracting three times repeatedly, the recovery rate of the method is guaranteed;And using ultra-high phase liquid chromatography triple quadrupole mass spectrometry technology, guanidine morpholino salt hydrochloride in complex matrix of agricultural products is successfully detected, the extraction method is fast and simple, the quantitative limit of guanidine morpholino salt hydrochloride in tea is 0.1mg / kg, and the quantitative limit in other matrix is 0.05mg / kg, which is suitable for routine analysis, and can meet the quantitative detection requirements of guanidine morpholino salt hydrochloride in complex matrix of agricultural products.

This invention provides a dressing containing Ligusticum striatum microspheres and its preparation method, to solve the problem of safe and precise application of Ligusticum striatum extract or Ligustrazine in difficult-to-heal wounds; the dressing containing Ligusticum striatum microspheres and its preparation method, wherein the dressing is composed of Ligusticum striatum microspheres loaded with pHEMA hydrogel as the main body, wherein the Ligusticum striatum microspheres are formed by coating Ligusticum striatum extract or Ligustrazine with PLGA, and the pHEMA hydrogel partially encapsulates the Ligusticum striatum microspheres.

The invention provides an extraction method of molybdenum trioxide, and belongs to the technical field of extraction metallurgy. The extraction method of molybdenum trioxide provided by the invention comprises the following steps: carrying out vacuum melting on a molybdenum-containing material to obtain a sublimate, and cooling the sublimate to obtain molybdenum trioxide, the molybdenum content in the molybdenum-containing material is 37.42 to 46.31 wt%, and the oxygen content in the molybdenum-containing material is 30.74 to 38.13 wt%; the temperature of vacuum melting ranges from 880 DEG C to 1200 DEG C, and the vacuum degree ranges from 0.47 Pa to 10 Pa; the cooling rate of the cooling is 5-10 DEG C / min. According to the method, the molybdenum-containing material is subjected to vacuum melting, molybdate in the molybdenum-containing material is decomposed to obtain molybdenum trioxide, original molybdenum trioxide of the molybdenum-containing material is sublimated under the high-temperature condition, high-purity molybdenum trioxide is obtained through cooling, and the extraction efficiency of molybdenum trioxide is effectively improved; and the extraction method is simple, the low-grade molybdenum-containing material is used as the raw material, the extraction cost is lower, and no pollution is caused to the environment.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The invention belongs to the technical field of environmental protection, relates to a preparation method and application of a natural organic corrosion inhibitor, and mainly aims to solve the problem of solid interface corrosion caused by long-term contact between metal equipment and a solution interface. Particularly, the problem of contact physical and chemical damage caused by instruments working in a high-concentration calcium salt environment and calcium chloride refrigerant pipelines is solved. The corncob is used as a source of a natural corrosion inhibitor, and effective components in the corncob are extracted into a hydrochloric acid ethanol solution. And then a solution with moderate molecular weight is obtained by means of molecular weight cutting, and is compounded with amino acid, so that the natural organic corrosion inhibitor is obtained. The extraction method is simple, convenient, economical and environment-friendly, is suitable for corrosion protection of reinforced concrete in a high-salt corrosion environment, and has a wide prospect.

The invention provides gardenia polysaccharide, a preparation method thereof and application of the gardenia polysaccharide in preparation of medicines for treating pulmonary fibrosis, and relates to the technical field of traditional Chinese medicine extracts and biological medicines. The gardenia polysaccharide GP121 with a new polysaccharide structure is extracted from gardenia, and the gardenia polysaccharide GP121 provided by the invention is simple in extraction method, high in polysaccharide extraction rate and low in production cost. The invention further researches the anti-pulmonary fibrosis biological activity of the gardenia polysaccharide GP121 on the basis of the cellular level, and the research result shows that the gardenia polysaccharide GP121 provided by the invention has the effect of delaying the MRC-5 cell fibrosis process caused by TGF-beta1; the phosphorylation of the Smad3 protein is inhibited, so that the expression of alpha-SMA, CollagenI and FN protein is reduced, and the fibrosis process is delayed. The gardenia polysaccharide GP121 provided by the invention can be used as a potential therapeutic drug for treating pulmonary fibrosis, and a new application of the gardenia polysaccharide in the aspect of treating pulmonary fibrosis is provided.

The invention provides an extracting agent and an extracting method of microalgae pigment. The extracting agent comprises 30-80% of polyol polymer or glucan, 1-5% of ionic liquid or eutectic solvent and water. And putting the algae powder into an extracting agent, and preserving heat to obtain the microalgae pigment. The method is a one-step method, the pigment is extracted from the microalgae powder by using an ionic liquid-polymer aqueous solution, an organic solvent is not used, a cell wall breaking step is reduced, and green solid-liquid extraction is realized.

The invention relates to the technical field of natural language processing, and particularly discloses an information extraction method and system, electronic equipment and a computer readable storage medium, the method comprises the following steps: determining a topic type of to-be-extracted information, and preprocessing the to-be-extracted information to obtain the preprocessed to-be-extracted information; based on the topic type and the preprocessed to-be-extracted information, extracting a plurality of key information fragments, and integrating all the key information fragments to obtain integrated information; and when the integrated information is compliant, determining the integrated information as target information. The accuracy and efficiency of information extraction are improved, and the method can be applied to the field needing efficient and accurate information processing.

The invention provides a plant glycosphingolipid extraction method, which comprises: taking a plant material, grinding liquid nitrogen, adding a hot ethanol solution (containing beta-mercaptoethanol), carrying out oscillation extraction at a high temperature, carrying out high-temperature high-speed centrifugation, taking the supernatant, carrying out low-temperature precipitation, and carrying out high-speed centrifugation to obtain a crude extract; washing the crude extract with acetone and diethyl ether, removing the organic solvent through high-speed centrifugation, adding a dissolving solution (tetrahydrofuran, methanol and water), carrying out vortex uniform mixing, carrying out high-temperature dissolution, filtering, and carrying out vacuum centrifugal drying on the filtrate to obtain a crude product; adding n-butyl alcohol and water into the crude product, dissolving, precipitating and centrifuging at high temperature, taking an upper layer n-butyl alcohol phase, and performing vacuum centrifugal drying to obtain a target product. The extraction method provided by the invention has the advantages of high extraction efficiency and time saving, and more importantly, small-weight plant material extraction can be effectively realized by virtue of simple equipment, and the sample size required by mass spectrum identification of GIPCs composition and ion chromatography identification of GIPCs glycosyl composition can be obtained.

The invention belongs to the technical field of compound traditional Chinese medicine preparation optimization algorithms and preparation optimization, and relates to a traditional Chinese medicine preparation optimization method based on a hemorheology mechanism and a preparation. Particularly relates to an optimization method of an antithrombotic yang-tonifying and recuperating decoction preparation and the preparation. According to the preparation optimization method, an intestinal flora regulator substance group and an anti-thrombus component substance group of medicinal material chemical components of the Yang tonifying and also five decoction and corresponding substance clusters of products obtained by different extraction methods are constructed, a better Yang tonifying and also five decoction extraction method is determined on the basis of intersection comparison of different substance groups, and then optimization is performed. The method can effectively guide the optimization of the Yang-tonifying and Yang-restoring decoction preparation. The Yang-tonifying Huanwu decoction preparation is prepared from a water decoction and alcohol precipitation extracting solution of raw radix astragali, radix paeoniae rubra and lumbricus and a 70% ethanol reflux extracting solution of the other four medicinal materials. The extracted components have the most intersection substances with the intestinal flora regulator substance group and the antithrombotic component substance group and the most intersection substances with high content, and have higher anticoagulant and antithrombotic effects.

The present invention discloses a method for extracting lithium from lepidolite, comprising: crushing the lepidolite and placing it in an aluminum salt solution for pressure leaching, filtering to obtain a first filtrate and a filter residue; removing iron from the first filtrate using an iron removal agent, filtering to obtain a second filtrate and iron-containing slag; removing fluorine and aluminum from the second filtrate, filtering to obtain a third filtrate and cryolite; precipitating lithium from the third filtrate, filtering to obtain lithium carbonate. The present invention is based on the coordination leaching of lithium from lepidolite by an aluminum salt system, achieving one-step leaching of lepidolite, the extraction method has a high yield, and the lithium carbonate finally obtained has a high purity; while extracting lithium carbonate, byproducts such as goethite and cryolite with high purity can be obtained to avoid waste. The extraction method of the present invention is simple, and the reagents used are all commercially available common reagents, with low cost and good lithium extraction effect, and is convenient for use in industrial production.

The invention discloses a method for extracting uranium from monazite high-density acid-soluble ore pulp, which comprises the following steps: adding a surfactant into the acid-soluble ore pulp, and sequentially carrying out aging and separation treatment to obtain supernate and underflow ore pulp; carrying out plate-frame filter pressing on the underflow ore pulp to obtain insoluble slag and filter pressing clear liquid; mixing and conveying the supernate and the pressure filtration clear liquid to a uranium resin exchange tower for uranium adsorption to obtain uranium saturated resin and adsorption tail liquid; the uranium saturated resin is eluted through a washing solution, and eluted qualified liquid and lean resin are obtained; heating the leaching qualified liquid, adding sodium sulfate, carrying out heat preservation reaction after the sodium sulfate is completely dissolved, adjusting the pH value of the solution to be 1.5-2 all the time, and carrying out solid-liquid separation to obtain uranium precipitation mother liquor and precipitation iron slag; and adding a sodium hydroxide solution into the uranium precipitation mother liquor to adjust the pH value to 7-8, and filtering to obtain sodium diuranate precipitation. The method aims at improving the uranium extraction rate in the monazite concentrate acid-soluble ore pulp.

This disclosure relates to a method for extracting β-nicotinamide mononucleotide from microalgae and the microalgae extract. The method includes: mixing microalgae raw material with an extractant for extraction; wherein the microalgae raw material is selected from one or more of Chlorella, Spirulina, and Haematococcus pluvialis; the extractant comprises 0.05–1.5 wt% of a nonionic surfactant, 0.5–1.0 wt% of an organic acid, 20–40 wt% of an alcohol, and the balance being a solvent. Microalgae raw materials are easy to cultivate, grow rapidly, have high protein content, and high β-nicotinamide mononucleotide content; the extraction method disclosed herein is simple, has a high extraction rate, and is low in cost.

The invention provides a ganoderma lucidum probiotic-containing fermented whitening beverage and a preparation method thereof, and belongs to the technical field of beverages. Chinese wolfberry fruits, heartleaf houttuynia herbs, radix angelicae and Chinese angelica are subjected to lactic acid bacteria fermentation separation to obtain traditional Chinese medicine fermented sugar and a fermentation extract, protein peptide extracted from nostoc sphaeroids kutz and the traditional Chinese medicine fermented sugar are subjected to a Maillard reaction, calcium and magnesium ions are chelated, and a Ca / Mg chelated Maillard reaction product is obtained; and mixing with selenium-rich ganoderma lucidum fermentation extract, the fermentation extract and trehalose, adding into purified water, sterilizing, and filling to obtain the ganoderma lucidum probiotic fermented whitening beverage. The ganoderma lucidum probiotic fermented whitening beverage prepared by the preparation method is a compound beverage, has relatively good antioxidant, anti-inflammatory, whitening and anti-aging effects, is relatively good in taste, relatively long in shelf life and convenient to store, is deeply loved by consumers, and has a wide application prospect.

The present invention discloses seafood mushroom sweet peptides and an extraction method and application thereof, and belongs to the technical field of protein active peptides. The amino acid sequence of seafood mushroom sweet peptides is: Glu‑Gly‑Asn‑Pro‑Ala‑His‑Gln‑Lys, Gln‑Glu‑Gln‑Glu‑Ala‑Lys‑Lys‑Arg or Ala‑Gly‑Leu‑Glu‑Leu‑Leu‑Lys. The sweet peptides are all extracted from seafood mushrooms. The sweet peptides are small molecular weight peptides and are easily absorbed by the human body. The sensory test results show that the extracted sweet peptides have good sweetness and richness, can be used instead of sucrose, and have great application significance in the food field.

The invention provides a method for automatically extracting polybrominated organic pollutants in a bottom mud sample, which comprises the following steps: step 1, collecting the bottom mud sample, wrapping the bottom mud sample with an aluminum foil, and performing low-temperature cryopreservation; 2, freeze-drying the sample by using a freeze-drying machine, and then sieving by using a metal sieve to remove impurities; step 3, performing parameter and process setting on an automatic sample pretreatment system built in a laboratory; 4, mixing the pretreated bottom mud with diatomite, filling the mixture into a stainless steel extraction pool, placing a filter membrane at the bottom of the pool body, placing the pool body into a sample extraction position of an accelerated solvent extraction instrument, and extracting a sample to obtain a bottom mud extracting solution; step 5, each time one sample is extracted, the obtained extracting solution is automatically transferred to a full-automatic gel purification quantitative concentration system instrument by a mechanical arm to be concentrated and dissolved, and then is transferred and submitted to a gel permeation chromatography by a sample injection needle of the instrument; step 6, concentrating the extracting solution, and eluting and purifying by using gel permeation chromatography; and 7, introducing the concentrated solution purified by the gel permeation chromatography into a concentrator through an internal pipeline, concentrating in two modes of pressure reduction and heating, and finally transferring the concentrated solution into a brown small sample injection bottle through a sample injection needle to obtain an extracting solution of the sample. The invention aims to provide a method for efficiently and quickly extracting brominated organic pollutants in bottom mud, manual operation is less, the sample pretreatment time is shortened, the extraction efficiency is improved, and convenience is provided for subsequent compound analysis and identification.

The invention belongs to the technical field of biological tissue engineering, and relates to APOA2-loaded platelet apoptosis vesicles as well as a preparation method and application thereof. According to the platelet apoptosis vesicle loaded with APOA2, protein APOA2 is loaded into apoptosis vesicles derived from platelets. According to the APOA2-loaded platelet apoptosis vesicle and the preparation method thereof disclosed by the invention, the prepared APOA2-loaded platelet apoptosis vesicle can better inhibit adipogenic differentiation of mesenchymal stem cells, so that liver fat accumulation can be safely and efficiently prevented, and the APOA2-loaded platelet apoptosis vesicle can be better used for treating non-alcoholic fatty liver diseases.

The invention discloses an application of sphingomonas extracellular polysaccharide in treatment of glucose and lipid metabolism disorder. The application range of the sphingomonas exopolysaccharide is expanded, the source is natural and safe, and a new thought is provided for prevention and treatment of diseases.

The application discloses a preparation method of trans-cuparemic acid, which comprises the following steps: step one, crushing and mixing; step two, extracting by heating and refluxing with ethyl acetate; step three, secondary extraction; step four, reducing pressure concentration; step five, elution treatment; and step six, concentration and recrystallization; wherein in the step one, cupressaceae plants are dried and crushed, and then screened; subsequently, the screened cupressaceae plant powder is uniformly mixed with equal amount of calcium carbonate powder to obtain mixed powder, which is prepared for use; in the step two, appropriate mixed powder obtained in the step one is weighed, and a certain amount of ethyl acetate is added to obtain a mixture; subsequently, the obtained mixture is boiled by heating for 2 hours, and a first extraction liquid is obtained by filtering; the application can effectively avoid pigment interference, and only one column chromatography operation is needed in the separation process; the obtained trans-cuparemic acid has large yield and high purity, and can be produced on a large scale.