5results about How to "Simple extraction method" patented technology

The present application relates to the field of analytical chemistry, and provide a kind of method for detecting complex matrix of agricultural products in guanidine morpholino salt hydrochloride.The extraction solvent of the present application uses acetonitrile+acid+aqueous solution, different acid is selected according to the water content of sample, trifluoroacetic acid is used for high water content sample, and formic acid is used for low water content sample;In purification, pH value is adjusted according to the oil content of sample, ammonia is used to adjust pH to 8.5 for high oil content sample, and pH value can not be adjusted for low oil content sample.In the method, NaCl is not added to make organic phase and aqueous phase in extraction liquid stratified, through the way of extracting three times repeatedly, the recovery rate of the method is guaranteed;And using ultra-high phase liquid chromatography triple quadrupole mass spectrometry technology, guanidine morpholino salt hydrochloride in complex matrix of agricultural products is successfully detected, the extraction method is fast and simple, the quantitative limit of guanidine morpholino salt hydrochloride in tea is 0.1mg / kg, and the quantitative limit in other matrix is 0.05mg / kg, which is suitable for routine analysis, and can meet the quantitative detection requirements of guanidine morpholino salt hydrochloride in complex matrix of agricultural products.

This invention provides a dressing containing Ligusticum striatum microspheres and its preparation method, to solve the problem of safe and precise application of Ligusticum striatum extract or Ligustrazine in difficult-to-heal wounds; the dressing containing Ligusticum striatum microspheres and its preparation method, wherein the dressing is composed of Ligusticum striatum microspheres loaded with pHEMA hydrogel as the main body, wherein the Ligusticum striatum microspheres are formed by coating Ligusticum striatum extract or Ligustrazine with PLGA, and the pHEMA hydrogel partially encapsulates the Ligusticum striatum microspheres.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

This disclosure relates to a method for extracting β-nicotinamide mononucleotide from microalgae and the microalgae extract. The method includes: mixing microalgae raw material with an extractant for extraction; wherein the microalgae raw material is selected from one or more of Chlorella, Spirulina, and Haematococcus pluvialis; the extractant comprises 0.05–1.5 wt% of a nonionic surfactant, 0.5–1.0 wt% of an organic acid, 20–40 wt% of an alcohol, and the balance being a solvent. Microalgae raw materials are easy to cultivate, grow rapidly, have high protein content, and high β-nicotinamide mononucleotide content; the extraction method disclosed herein is simple, has a high extraction rate, and is low in cost.

The application discloses a preparation method of trans-cuparemic acid, which comprises the following steps: step one, crushing and mixing; step two, extracting by heating and refluxing with ethyl acetate; step three, secondary extraction; step four, reducing pressure concentration; step five, elution treatment; and step six, concentration and recrystallization; wherein in the step one, cupressaceae plants are dried and crushed, and then screened; subsequently, the screened cupressaceae plant powder is uniformly mixed with equal amount of calcium carbonate powder to obtain mixed powder, which is prepared for use; in the step two, appropriate mixed powder obtained in the step one is weighed, and a certain amount of ethyl acetate is added to obtain a mixture; subsequently, the obtained mixture is boiled by heating for 2 hours, and a first extraction liquid is obtained by filtering; the application can effectively avoid pigment interference, and only one column chromatography operation is needed in the separation process; the obtained trans-cuparemic acid has large yield and high purity, and can be produced on a large scale.