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This invention provides a dressing containing Ligusticum striatum microspheres and its preparation method, to solve the problem of safe and precise application of Ligusticum striatum extract or Ligustrazine in difficult-to-heal wounds; the dressing containing Ligusticum striatum microspheres and its preparation method, wherein the dressing is composed of Ligusticum striatum microspheres loaded with pHEMA hydrogel as the main body, wherein the Ligusticum striatum microspheres are formed by coating Ligusticum striatum extract or Ligustrazine with PLGA, and the pHEMA hydrogel partially encapsulates the Ligusticum striatum microspheres.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.