711 results about "Solid-phase microextraction" patented technology

A device for carrying out solid phase microextraction is a tubular member having one closed end and one open end with an extracting surface within said tubular member. The extracting surface can be an extracting phase coating extending over a zone within the tubular member. The tubular member has a heater that can be used for either or both drawing fluid carrier into the tubular member or assisting in desorbing analytes into an injector of an analysis instrument. The tubular member can also be mounted in a housing with an airtight cavity and a plunger. A method of operation of the device is also provided. Previously, samples were analyzed using liquid-liquid extraction or using cartridges. Both of these methods are relatively expensive and time consuming. Both of these methods also require the use of solvents which can be difficult and expensive to dispose of.

Applicants' teachings relate to a method and device to extract components contained in a fluid. More particularly, the applicants' teachings are directed towards a method and sampling device for quick extraction of targeted components from their in vivo surroundings. In accordance with various embodiments of applicants' teachings a sampling device and method to extract components, such as, for example, analytes, from a fluid is provided. The device is interfaced with a fluid source, such as, for example, but not limited to, a circulatory system of an animal, to enable extraction of target molecules with minimal loss of fluid (e.g., blood) to the source. The device facilitates an interface between the circulatory system of an animal and a suitable sampling probe such as, for example, a solid phase microextraction (SPME) probe. The targeted compounds are desorbed in small volumes of solvents that can be analyzed with highly specific instruments. In particular, in various embodiments of applicant's teachings a device to extract components contained in a fluid is provided. The device comprises a housing defining a cavity, with the housing having at least one opening to allow fluid flow into and out of the cavity. The device also comprises a probe insertable into the cavity of the housing. Moreover, the device comprises a channel, with the channel adapted to connect the cavity to a fluid pump, so that when the probe is inserted into the cavity the fluid pump causes fluid to flow into and out of the cavity through the at least one opening and contact the probe in the cavity.

A metal-organic framework (MOF) polymer for solid-phase microextraction (SPME) includes an MOF including metal ions building units coordinating polytopic organic linkers; and a polymer coordinatively bonding to the MOF, wherein the polymer is composed of one or more vinyl monomers and a cross-linker those are polymerized in the presence of a radical initiator. A method for preparing a stationary phase for SPME is also provided.

The invention discloses a device and a method for the combined use of molecular imprinting solid phase microextraction and hollow fiber liquid phase microextraction, and the application thereof. The device consists of a solid phase microextraction device, a molecular imprinting solid phase microextraction probe, a hollow fiber membrane, an extraction bottle and a stirrer. The molecular imprintingsolid phase microextraction probe is inserted in to a hollow fiber membrane cavity containing organic solvent, fixed and then directly positioned into a sample solution to be measured for purpose of liquid-solid phase synchronous microextraction. During the microextraction, analytes permeate the hollow fiber membrane, are then enriched in the organic solvent and then undergo secondary extraction and enrichment under the action of the molecular imprinting solid phase microextraction probe. The device is simple, operates conveniently, facilitates the combined use with an analyzer, is suitable for the separation and enrichment of organics with trace amounts in complex water phase samples obtained from the fields such as environment, food, medicine, biology and the like.

The invention discloses a chiral analysis method for nicotine in cigarette cut tobacco. The chiral analysis method for nicotine in cigarette cut tobacco is characterized by including the steps that cut tobacco samples are dried, smashed and screened, extracting liquid is added, the mixture is extracted and filtered, then common cut-tobacco-sample extracting liquid is directly analyzed through a multidimensional gas phase chromatography and mass spectrometry detector, cut-tobacco-sample extracting liquid with the excessively-low nicotine content is subjected to automatic solid phase microextraction to be subjected to multidimensional gas-phase-chromatography tandem-mass-spectrum analysis, and the proportion of S-(-)-nicotine and R-(+)-nicotine in the cigarette cut tobacco is quantitatively detected with the peak area normalization method. The detection method is simple, fast and convenient in sample treatment, low in method detection limit, high in sensitivity and good in accuracy degree, and analysis of cigarette cut tobacco samples can be met.

The invention discloses a continuous gas in-tube solid phase microextraction device combined with gas chromatography (GC) for use. The continuous gas in-tube solid phase microextraction device comprises a continuous gas in-tube solid phase microextraction device and a sampling device, wherein the continuous gas in-tube solid phase microextraction device is composed of an in-tube solid phase microextraction needle, a semiconductor condenser, a microheater and an inert gas connecting pipe, and the sampling device is composed of the in-tube solid phase microextraction needle, and a stop valve, a carrier gas flow controller and a gas source which are connected in sequence through a connector connected with a port of an extraction needle on one end of the in-tube solid phase microextraction needle and are used with the gas chromatography. According to the invention, the extraction needle performs thermal desorption at a GC sample inlet without changing the GC sample inlet and thus the continuous gas in-tube solid phase microextraction device is applied to the GC with any brand. The invention has the advantages of simple operating method, high extraction rate, accurate quantification, hydrophilic environment, convenient automatic operation and the like and also provides a sample pretreatment method integrated with extraction, separation and concentration. The continuous gas in-tube solid phase microextraction device can greatly promote the combination use of the SPME (solid phase microextraction) technology and the GC/MS (mass spectra) technology and applications of micro constituents in complex samples in aspects of on-site analysis, on-line analysis and in-situ analysis.

The invention discloses a method for rapidly detecting polybrominated diphenyl ether residue in a sample, particularly in textiles. The method is characterized by comprising the following steps: (1) the sample is crushed, dipped in base solution and ultrasonically extracted in water bath, and solid phase microextraction is employed to concentrate a target compound; and (2) polybrominated diphenyl ether is qualitatively and quantitatively determined by gas chromatography-mass spectrometry after thermal desorption. The method helps effectively solve the problem of matrix effect that fabric fibers have no solvent extraction, obtains a satisfactory result for the analysis of a selected target compound, has the advantages of low organic solvent consumption, rapid extraction speed, less interference from impurities, high sample recovery rate and the like, and can be applicable to rapidly detecting polybrominated diphenyl ether flame retardant residue in textiles.

Disclosed is a preparation of an SPME fiber. A covalent bond method is adopted to prepare metal-organic frameworks (MOFs) as a coating for the extraction fiber. The fiber preparing method includes performing APTES amino-functionalization on a quartz fiber, namely, placing the fiber with amino on the surface into an MOF solution for reaction. An extraction device assembled by the prepared SPME coating comprises a device body, a microsyringe outer tube, a microsyringe needle, a push-pull rod and the extraction fiber with the coating, wherein the push-pull rod is arranged in the microsyringe outer tube and forms a sliding matching with the inner wall of the tube, the lower end of the push-pull rod is corroded by an aqua regia solution to obtain a corrosion end which is inserted into the extraction fiber with the coating, and a handle is arranged at the upper end of the push-pull rod. The preparation and the device have the advantages that an organic ligand of the extraction fiber coating and the MOFs is highly ordered and controllable, the extraction device has a novel structure, the coating fiber fixation is firm, and the stability and service life of the extraction fiber coating are improved.

A device for carrying out solid phase microextraction is a fiber contained in a syringe. The fiber can be solid or hollow. The syringe has a barrel and a plunger slidable within the barrel, the plunger having a handle extending from one end of the barrel. A hollow needle extends from an end of the barrel opposite to the plunger. The fiber is contained in the needle. When the plunger is depressed, the fiber extends beyond a free end of the needle and when the plunger is in a withdrawn position, the fiber is located within the needle. The syringe protects the fiber from damage. When it is desired to analyze a sample in a bottle having a septum, the needle is inserted through the septum and the plunger is depressed so that the fiber will extend into the sample. After one or two minutes, the plunger is moved to the withdrawn position so that the fiber will return to the needle and the syringe is withdrawn from the sample bottle. The syringe is then inserted through a septum in a gas injection port of a gas chromatograph. The plunger is again depressed so that the fiber will extend into the gas chromatograph and an analysis of the components on the fiber is carried out. Then, the plunger is moved to the withdrawn and the syringe is withdrawn from the injection port. Previously, samples were analyzed using liquid-liquid extraction or using cartridges. Both of these methods are relatively expensive and time consuming. Both of these methods also require the use of solvents which can be difficult and expensive to dispose of.

The solid phase capillary miniature extractor includes mainly one four-way valve and one six-way valve connected via gas path; one capillary extracting column made of elastic hollow quartz capillary, stainless steel capillary or stainless steel capillary with inside quartz lining, connected to the six-way valve and with cross-linking coated fixing phase in the inside wall; one heater outside the extracting column for heating; one miniature three-way pipe connected to the extracting column, the six-way valve and conveying gas path for the desorption sample; and one conveying pipe inserted into the gas chromatographic sample injector. The liquid sample pushed by the auxiliary carrier gas enters first to the extracting column via the four-way valve and the six-way valve; the four-way valve is then turned and the extracting column is heated; and the six-way valve is finally turned for the desorbed component to enter the gas chromatographic sample injector.

The invention provides an analytical method for determining aromatic constituents in tea. The method adopts HS-SPME (headspace-solid phase microextraction), combines GC-MS (gas chromatography-mass spectrometer) with the Kovats retention index (KI), employs a 50/30mu m DVB/CAR/PDMS type SPME head, optimizes HS-SPME parameters, establishes an AMDIS (automated mass spectral deconvolution and identification system) combined with the KI, and establishes a tea aroma atlas database for analysis. Compared with the conventional tea aroma analysis, the provided analytical method is better in accuracy, efficiency, flexibility and comprehensiveness, and can be effectively applied to qualitative and quantitative analysis for identification of the aromatic constituents in the tea.

A device for carrying out solid phase microextraction on-site is a tubular member having one closed end and one open end with an extracting surface within said tubular member. The extracting surface can be an extracting phase coating extending over a zone within the tubular member. The tubular member is mounted in a housing with an airtight cavity. A method of operation of the device is also provided. The solid phase microextraction device facilitates the ultimate goal of chemist to perform analysis on-site at place where a sample is located rather than moving the sample to laboratory, as it is a common practice in many cases at present. This approach eliminates errors and reduces the time associated with sample transport and storage and, therefore, it results in more accurate, precise and faster analytical data.

The invention discloses a solid phase microextraction coating of a hydroxyl cucurbituril as well as a preparation method and an application thereof, relates to an analyzing sample pre-treatment technology, and belongs to the technical field of analytical chemistry. The solid phase microextraction coating is characterized in that a common pentabasic cucurbituril or six-membered cucurbituril is subjected to potassium peroxodisulfate oxidation and cation exchange resin separation and purification so as to obtain the hydroxyl cucurbituril through, the hydroxyl cucurbituril is bonded on a sol-gel mesh through a chemical reaction so as to obtain the solid phase microextraction coating, the coating is utilized to prepare a solid phase microextraction fibre which is combined with a gas chromatography, the environmental water sample containing polycyclic aromatic hydrocarbon is analysed, and the coating is utilized to prepare a solid phase microextraction stirring rod which is combined with a high performance liquid chromatography so as to analyze non-steroid antiinflammatory drugs. The solid phase microextraction coating provided by the invention has the advantages that the superficial area of the coating is large, and the extraction capacity is large; and the solid phase microextraction coating is a novel solid phase microextraction coating, is used for analyzing, high in selectivity, low in detection limitation, wide in linearity range, and good in selectivity.

An object of the present invention is to provide a packing material for solid phase extraction, ensuring excellent recovery of not only a hydrophobic substance but also an ionic substance by having hydrophobicity and an ion exchange group at the same time, and a solid phase extraction method, a packing apparatus, and a method for treating a sample, using the packing apparatus. A packing material for solid phase extraction of the present invention is a particle obtained by copolymerizing a hydrophobic monomer (A) and a hydrophilic monomer (B) and introducing thereinto an ion exchange group, in which the ion exchange group is introduced without impairing the hydrophobic site. The present invention also provides a solid phase extraction method, a packing apparatus for solid phase extraction and a method for treating a sample, using the packing material.

A biocompatible coating for solid phase microextraction (SPME) of a small molecule from a biological matrix. The coating comprises SPME particles and a biocompatible polymer. The biocompatible polymer (e.g. polyacrylonitrile) reduces adsorption of proteins or macromolecules onto the SPME particles and allows the SPME particles to extract the small molecule from the matrix. A process for coating a flexible fiber with a biocompatible coating. The process comprises: coating the fiber with a suspension of SPME particles, the SPME particles being suspended in a solution of a biocompatible polymer and a solvent, the biocompatible polymer can comprise polyacrylonitrile (PAN); drying the coated fiber to remove the solvent; and curing the dried coated fiber at an elevated temperature.

The invention is concerned with the prepare method of capillary for solid phase microextraction. The methacrylic acid glycidyl is function monomer, the ethyleneglycol dimethacrylate is vulcanizing agent, toluene and dodecyl are mixed pore-causing agent, and 2,2'-azobisisobutyronitrile is initiator. Mix and mill the stuff and pour into quartz capillary disposed with ethylene, close and heat to polymerization. After the reaction, use solvent to clean capillary and remove the rudimental monomer, vulcanizing agent and pore-causing agent to get whole capillary with the polymer of methacrylic acid glycidyl and ethyleneglycol dimethacrylate. Use vitriol to clear the whole capillary to get hydroxylation, or clear the capillary with diethylamine and close to heat at 65 to 75 degree to get the capillary with the polymer of methacrylic acid glycidyl and ethyleneglycol dimethacrylate derived from diethylamine. The method is easy and low cost and the extraction agency stuff is fit to set up a credible and high sensitive analysis method.

The invention discloses a method for simultaneously detecting six types of 24 antibiotics in water by solid phase extraction-liquid chromatography-tandem mass spectrometry. The method comprises (1) carrying out water sample pretreatment through adding an internal standard substance indicating a recovery rate before solid phase extraction, (2) enriching and purifying a target antibiotic with a solid phase extraction small column and (3) detecting contents of six types of 24 antibiotics in water through a liquid chromatography-tandom mass spectrometer. The method can simultaneously detect residues of six types of 24 antibiotics in water, has low time consumption in detection and has the advantages of high precision, high sensitivity, high stability, high selectivity and low detection limit.In the water sample pretreatment, an organic reagent use amount is small, environmental friendliness is realized, operation is simple, an enrichment factor is high and reproducibility is good. The method utilizes the internal standard substance indicating a recovery rate before solid phase extraction, well shows the loss of the target substances in the pretreatment and produces a real and reliablefinal result.

An automatic solid-phase microextraction (SPME) sampling apparatus having a mobile sampler arm with a holder for a probe carrying a fiber 9 and an intermediate bracket for transferring the probe between a probe storage tray and the gas chromatograph injector. In the various probe handling stages, the probes are collected by a magnetic member attached to the end of the plunger in the holder, at the free end of the holder's body and at the lower end of the block for guiding the probe needle. The magnetic member interacts with a ferromagnetic connector attached to one end of the fiber and with a ferromagnetic flange attached to the end of the needle containing the fiber in order to place the probe on the transfer bracket or remove it therefrom. The apparatus can perform analyses on fibers that have already been exposed, extract analytes from samples, and subsequently transfer them for desorption in the gas chromatograph in a fully automatic way.

The invention discloses a method for rapidly determining the fragrance components of tea quantitatively and qualitatively. The method is characterized by mainly comprising two steps: (1) extracting fragrance components: taking ethyl caprate as the internal standard substance, and taking a headspace solid-phase micro-extraction technology to extract fragrance components from tea soup to obtain a solid phase extraction head with enriched fragrance components; (2) quantitative and qualitative analysis of fragrance components: analyzing the solid phase extraction head with enriched fragrance components obtained in the step (1) by gas chromatography-mass spectrum (GC-MS), wherein the qualitative analysis is that common characteristic peaks are used to construct a standard fingerprint of fragrance components in tea; and the quantitative analysis is that the area of internal standard substance namely ethyl caprate is taken as the reference, and according to the peak areas of common characteristic peaks, each fragrance component of tea can be quantified. According to the method, a headspace solid phase micro-extraction technology and a GC-MS combined retention index method are used to rapidly analyze the fragrance components of tea, and by effectively combining the mass spectrum searching, the reliability and accuracy of quantitative analysis and qualitative analysis are largely improved.