85 results about "Solid-phase microextraction" patented technology

This application relates to the technical field of gas detection, and particularly to a method for detecting the concentration of specific gases in explosion - hazardous areas. This method uses solid - phase microextraction sampling on - site. The collected samples are detected offline using a surface acoustic wave gas chromatograph, and the gas concentration of the target component in the explosion - hazardous area is calculated and analyzed based on the detection results, ultimately achieving rapid detection of the concentration of specific gases in explosion - hazardous areas. The method for analyzing the concentration of specific gases in explosion - hazardous areas by coupling SPME with GC - SAW proposed in this application does not require pretreatment of the samples collected by SPME and can be directly injected for detection. The sampling and detection methods are safe, and the entire detection time cycle is greatly shortened.

The invention discloses a solid-phase microextraction derived fiber head and a preparation method and application thereof, the solid-phase microextraction derived fiber head comprises a solid-phase matrix and polystyrene microspheres adhered to the surface of the solid-phase matrix, and a chitosan cross-linked network layer is also cross-linked on the polystyrene microspheres; cysteine is also adsorbed on the polypropylene microsphere and chitosan cross-linked network layer. According to the solid-phase microextraction derivative fiber head, a large number of hydrophilic action sites such as amino groups and carboxyl groups exist on the surface, so that synchronous extraction and detection of the contents of three target analytes, namely cysteine-chlorothalonil association compounds, sulfydryl chlorothalonil and hydroxychlorothalonil, are realized.

The invention discloses an ethylene oxide detection and analysis method, which belongs to the technical field of detection and analysis, and comprises the following steps: weighing a sample to be detected, adding deuterated internal standard ethylene oxide-D4, and dissolving the sample with a low-temperature mixed solvent to obtain a sample solution; uniformly mixing the sample solution, adding a purifying material for purifying, uniformly mixing and centrifuging to obtain supernate; putting a part of the supernate into a small solid-phase microextraction bottle, adding sodium chloride, and uniformly mixing to obtain a solution to be detected; placing the small solid-phase microextraction bottle filled with the solution to be detected on a gas chromatograph-tandem mass spectrometer with a CTC automatic solid-phase microextraction sampling device, and extracting and detecting the prepared solution to be detected; and analyzing the data acquired by the mass spectrum by utilizing Agilent MassHunter data processing software. According to the method, volatile components in a sample are enriched, concentrated and then detected, the residual amount of ethylene oxide in the fat-soluble plant extract can be directly and quantitatively analyzed, the sensitivity is high, and the accuracy is good.

The invention belongs to the field of chemical detection, and particularly relates to a method for detecting ethylene oxide, 2-chloroethanol and acetaldehyde in a frozen beverage by solid-phase microextraction gas chromatography-mass spectrometry. The invention relates to a method for detecting ethylene oxide, 2-chloroethanol and acetaldehyde in a frozen beverage by solid-phase microextraction gas chromatography-mass spectrometry, which comprises the following steps: S.1) taking a frozen beverage sample to be detected, homogenizing and uniformly mixing; s.2) weighing a certain amount of sample, and adding sodium chloride and hydrochloric acid; s.3) determining the sample by adopting a solid-phase microextraction gas chromatography-mass spectrometry method; and S.4) quantitatively analyzing the contents of ethylene oxide, 2-chloroethanol and acetaldehyde in the frozen drink sample to be detected by adopting an external standard method. According to the method disclosed by the invention, the derivatization reaction and the solid-phase microextraction process are combined into one, and the difficulties of complicated pretreatment, long time consumption, low accuracy, unstable result, high detection limit and the like in the traditional analysis technology are overcome. Meanwhile, the precision of a detection result is high, the influence of a complex matrix is removed, and the reproducibility is good.

This invention discloses a surface coating material, a solid-phase microextraction probe, its preparation method, and its application, relating to the field of veterinary drug detection technology. The probe comprises a metal rod and a coating applied to the outer surface of the metal rod. The surface coating material is prepared by electrospinning a mixture of SBA-15 material, polyacrylonitrile, and N,N-dimethylformamide. The ratio of SBA-15 material, polyacrylonitrile, and N,N-dimethylformamide is 100mg–300mg: 100mg–300mg: 2mL–4mL. This invention addresses the problems of inconvenient equipment portability and long detection times in existing technologies.

The application discloses a preparation method of zinc oxide nanoflower fibers and application thereof. The preparation method comprises the following steps: cleaning and acid treating a nickel-chromium alloy wire base; preparing a zinc oxide seed layer on the surface of the nickel-chromium alloy wire base through an immersion-calcination method; and growing zinc oxide nanoflowers on the seed layer in situ through a hydrothermal method to obtain the zinc oxide nanoflower fibers. The fiber has the nickel-chromium alloy wire as the base, is good in flexibility and high in strength, has a firm combination of the zinc oxide nanoflower coating on the surface, is large in specific surface area, has rich active sites, and exhibits excellent selective adsorption capacity to polycyclic aromatic hydrocarbons. The application also provides application of the fiber in solid-phase microextraction, in particular, application of the fiber in enrichment and detection of trace polycyclic aromatic hydrocarbons in environmental water samples. The fiber is used for solid-phase microextraction of polycyclic aromatic hydrocarbons and detection of trace polycyclic aromatic hydrocarbons in environmental water samples, is low in detection limit, is good in reproducibility, is suitable for environmental water monitoring, and has important practical application value.

The present application relates to the technical field of Fischer-Tropsch synthesis wax analysis chemistry, and in particular to a method for detecting oxygen-containing compound components in Fischer-Tropsch synthesis wax by using solid-phase microextraction and comprehensive two-dimensional gas chromatography-mass spectrometry. The method comprises the following steps: first, performing solid-phase microextraction on a to-be-tested wax sample to obtain an extract rich in oxygen-containing compound components; and then using comprehensive two-dimensional gas chromatography-mass spectrometry to qualitatively determine the composition of the oxygen-containing compound components contained in the extract. In the method, the content of the oxygen-containing compound components is less than or equal to 7 wt% based on the total weight of the Fischer-Tropsch synthesis wax. The method has the characteristics of high throughput, high sensitivity and high accuracy, and solves the problems of difficult analysis and separation and difficult qualitative determination of alcohol, aldehyde, ketone, acid, ester and other oxygen-containing compound components in Fischer-Tropsch synthesis wax.

The invention discloses a solid-phase microextraction probe based on a hollow covalent organic framework material and application of the solid-phase microextraction probe in lipidomics analysis, and relates to the technical field of lipidomics analysis. The preparation method of the hollow covalent organic framework material comprises the following steps: carrying out a Schiff base reaction on 1, 3, 5-tri (4-aminophenyl) benzene and trimesic aldehyde at 20-30 DEG C to obtain a covalent organic polymer; and dispersing the covalent organic polymer in an organic solvent, adding glacial acetic acid, and reacting at 65-75 DEG C to obtain the hollow covalent organic framework material. The hollow covalent organic framework material disclosed by the invention is high in matrix interference resistance and high in lipid extraction capacity, and can realize efficient enrichment of micromolecular lipid metabolites. The prepared SPME probe can be applied to in-vivo lipid sampling analysis and lipidomics research, and a more accurate and effective method is provided for real-time capture of in-vivo lipid abundance.

The application discloses a kind of phosphoric acid group modified β-cyclodextrin-based polymer film microextraction coating material and its application.First, a kind of phosphoric acid group β-CD porous crosslinked polymer (DBP-β-BNCD) and its preparation method are provided.The DBP-β-BNCD has the advantages of large specific surface area, narrow pore size distribution, large pore volume, good thermal stability, etc., which is beneficial to the accessibility of adsorption sites.When adsorbing hydrophilic pollutants antibiotics, various adsorption mechanisms such as polarity interaction, hydrogen bond interaction and π-π interaction are introduced.Further, by using DBP-β-BNCD as a thin film solid phase microextraction coating material, a method combining TFME with high performance liquid chromatography-tandem mass spectrometry (HPLC-QqQ-MS) is established, and the results show that the coating material has great potential for the extraction of hydrophilic organic pollutants in the environment.

The invention belongs to the technical field of antibiotic residue detection, and particularly relates to a rapid detection method for quinolone antibiotics in dairy products. The preparation method comprises the following steps: preparing gold nanoparticles as gold seeds by using a sodium citrate reduction method, epitaxially growing an ultrathin Cr-MIL-101-NH2 shell layer by using a layer-by-layer self-assembly method, and constructing an Au-coated Cr-MIL-101-NH2 NPs core-shell structure; a solid phase microextraction-surface enhanced Raman spectroscopy (SPME-SERS) combined method is established on the basis of the material, a shell layer is used for efficiently extracting and selectively enriching a target object to a gold nanoparticle local plasma resonance area, strong SERS signal enhancement is achieved, and the shell layer guarantees that the gold nanoparticles are stable in performance in a complex matrix at the same time. Methodology verification proves that the method is wide in linear range, good in precision, high in recovery rate, low in detection limit, convenient in pretreatment and suitable for rapid high-throughput detection of antibiotic residues in food.

The present invention provides a cacao bean processed product, a material for a food containing the cacao bean processed product, and a food containing the material for a food, wherein the cacao bean processed product contains: acetone; and at least one selected from 2-methylbutanal, 3-methylbutanal, and isopropyl alcohol, wherein the cacao bean processed product, when analyzed by solid phase microextraction-gas chromatography-mass spectrometry, satisfies at least one of the following (A) to (C):(A) a ratio of a peak area of acetone to a peak area of 2-methylbutanal is 5.0 or more;(B) ratio of a peak area of acetone to a peak area of 3-methylbutanal is 8.0 or more; and(C) a ratio of a peak area of acetone to a peak area of isopropyl alcohol is 1.2 or more.

This invention discloses a method for preparing a solid-phase microextraction (SPME) device coated with a porous carbon material (DPCM) derived from a covalent organic framework (COF). The method primarily includes the following steps: preparing DPCM; preparing a DPCM sol-gel solution; inserting treated fibers into the DPCM sol-gel solution, extracting and curing them, and repeating this process multiple times to produce a DPCM-coated fiber. This fiber is then secured in a 1 mL microsyringe to form a solid-phase microextraction device. Combined with gas chromatography, the device is used for the extraction and analysis of trace phthalates (PAEs) in water samples, significantly improving detection sensitivity.

The application relates to a preparation method of a solid-phase microextraction coating, comprising the following steps: (1) dissolving a metal organic framework material MIL-101Cr as an adsorbent and a binder in a solvent to prepare a suspension; (2) uniformly mixing and ultrasonically treating the suspension, then inserting a stainless steel substrate into the suspension and slowly taking out the substrate, so that a coating is coated on the substrate, and the process is repeated for several times until the coating on the substrate reaches a required thickness. Compared with commonly used commercial solid-phase microextraction heads, the solid-phase microextraction fiber coating for sample pretreatment has better extraction effect than a two-in-one extraction head and is equivalent to a three-in-one extraction head, and the prepared solid-phase microextraction fiber coating has a long service life and can be repeatedly used for 160 times.

A full-automatic solid-phase microextraction device comprises a base, a controller is arranged on one side of the upper portion of the base, a moving device is arranged on one side of the controller, a vibration stirring mechanism is arranged on the lower portion in the moving device, a probe is arranged below the vibration stirring mechanism and connected with a probe cotton swab, and a test tube tray is arranged on the other side of the upper portion of the base. According to the utility model, the semi-automatic treatment of large-batch adsorption, cleaning and desorption of the cotton swab solid-phase micro-extraction head is realized, the treatment efficiency is greatly improved, and an expensive full-automatic solid-phase extraction instrument does not need to be purchased; a column passing mode is replaced by an oscillation stirring mode (stirring while moving up and down), so that the problems that viscous and colloidal complex samples are easy to block and are not easy to elute are solved; the problems of poor liquid-liquid extraction purification effect and possible interference of a complex sample matrix are solved. The operation is greatly simplified, and the time is shortened to one third of the original time; the cost of the full-automatic solid-phase microextraction instrument is only one tenth of that of a traditional full-automatic solid-phase microextraction instrument.

The application discloses application of a zwitterionic covalent organic framework solid-phase microextraction fiber in neurotransmitter analysis, wherein the zwitterionic covalent organic framework material is prepared by Schiff base reaction condensation with ethidium bromide, 2,5-diaminobenzoic acid and triformylphloroglucin as functional monomers and acetic acid as a catalyst to obtain the zwitterionic covalent organic framework material. The coating of the zwitterionic covalent organic framework solid-phase microextraction fiber prepared in the application has good hydrophilicity, and the structure is rich in functional groups such as hydroxyl groups, carboxyl groups, phenyl groups and quaternary ammonium groups, which can interact with target analytes through ion exchange, pi-pi stacking, hydrogen bonding and hydrophilic-hydrophobic interaction, thereby showing strong specific adsorption and high enrichment capacity for neurotransmitters. The zwitterionic covalent organic framework solid-phase microextraction fiber prepared in the application realizes efficient enrichment and extraction analysis of neurotransmitters with different polarities in complex biological sample matrices.

The utility model provides a special fixing device for gas phase / gas chromatography-mass spectrometry solid phase microextraction, which relates to the technical field of laboratory devices and comprises an extraction component, a sample bottle clamping component and a solid phase microextraction clamping component are arranged on the extraction component, and the extraction component comprises a base. The solid-phase microextraction clamping assembly comprises a mounting plate and a fastening bolt. According to the utility model, the air cylinder is controlled to extend synchronously, so that the clamping plate is pushed to move, the solid-phase microextraction parts are limited by the clamping plate, the solid-phase microextraction parts with different sizes can be effectively fixed, the practicability and the flexibility are improved, and after the solid-phase microextraction parts are clamped and fixed by the clamping plate, the solid-phase microextraction parts are clamped and fixed by the clamping plate. The limiting plates penetrate through the interiors of the single clamping plates, the springs are stressed and compressed by pulling the limiting rods upwards, after the clamping plates clamp and fix the solid-phase microextraction part, the limiting rods are loosened, the limiting rods penetrate through the interiors of the single limiting grooves through the elasticity of the springs, and limiting is facilitated.

This application belongs to the technical field of mycotoxin detection, and specifically relates to the application of a magnetic solid-phase dispersion extractant in detecting the mycotoxin content in wheat. The magnetic solid-phase dispersion extractant is NH2-Fe3O4@TpBD-COF. As a magnetic solid-phase microextraction agent, NH2-Fe3O4@TpBD-COF adopted in the present invention only needs 10 minutes to complete the treatment process in the technical solution provided by the present invention, featuring a short required time; compared with the commonly used solid-phase microextraction column, the technical solution provided by the present invention only requires a usage amount of 10 mg, featuring low cost; compared with the disposable extraction column, NH2-Fe3O4@TpBD-COF adopted in the present invention can be reused 8 times after simple cleaning, featuring environmental friendliness.

The invention discloses a preparation method and application of a carboxyl functionalized MON (at) ZIF-8-NH2 solid phase microextraction material. The preparation method comprises the following steps: taking ZIF-8-NH2 as an inner core and MON-2COOH as a shell to obtain a carboxyl-functionalized MON-coated ZIF-8-NH2 material, then fixing the carboxyl-functionalized MON-coated ZIF-8-NH2 material on a substrate by adopting a physical coating technology to obtain a solid-phase micro-extraction coating fiber based on carboxyl-functionalized MON-coated ZIF-8-NH2, and using the extraction fiber to measure hydroxyl polycyclic aromatic hydrocarbon in urine. The solid-phase micro-extraction coating fiber disclosed by the invention is thinner in coating, better in extraction effect, longer in service life and higher in stability. The method can be used for extracting various hydroxyl polycyclic aromatic hydrocarbons in urine at the same time by being combined with liquid chromatography-mass spectrometry, and has good stability, meanwhile, the determination method has excellent accuracy, and the recovery rate and the detection limit meet the detection requirements of trace analysis.

The utility model belongs to the technical field of magnetic solid-phase microextraction, and relates to a simple magnetic solid-phase microextraction device for a laboratory. The device comprises a magnetic separation frame capable of processing samples in batches and flexibly controlling an external magnetic field, and a separation tube capable of rapidly separating sample liquid. The magnetic separation frame comprises a separation frame main body with an isosceles trapezoid longitudinal section, a handle, test tube grooves positioned on the front side and the rear side of the separation frame main body, a test tube positioning clamping groove, a test tube arc mask, an adjustable magnetic block clamping groove, a movable magnetic block, a magnetic block fixer, and a sample receiving groove comprising a first surface and a second surface of the sample receiving groove. The separation tube can quickly separate sample liquid and comprises a threaded tube cap, a separation tube body, a sealing head liquid guide tube, a through hole with an upper opening, a small liquid outlet and a handle. According to the device, the whole set of flow of magnetic solid phase extraction can be completed in one set of device, the operation is simple, tedious and repeated manual operation is reduced, batch sample treatment is met, and meanwhile, the defects of high cross contamination probability and poor controllability in the extraction process can be avoided.

The invention relates to the technical field of food analytical chemistry, and particularly discloses a method for analyzing volatile components in rice, which comprises the following steps: S1, aging a fiber head: aging a solid phase microextraction fiber head at a gas chromatography sample inlet at 270 DEG C for 30 minutes; s2, sample preparation: weighing 3g of a rice sample, putting the rice sample into a headspace sample injection bottle, adding 6mL of water, and sealing the headspace sample injection bottle; s3, headspace balancing: stirring and balancing the sealed sample injection bottle at the temperature of 80 DEG C at the speed of 250 rpm; s4, headspace extraction: placing the aged fiber head at a position 3 cm above the headspace of the sample, and carrying out adsorption extraction for 30 minutes; s5, thermal desorption: inserting the extracted fiber head into a GC-MS (Gas Chromatography-Mass Spectrometer) sample inlet, and desorbing for 6 minutes at 230 DEG C; and S6, performing chromatographic analysis on the desorbed sample. The method is simple, convenient and rapid to operate, capable of completing large-scale detection in a short time and wide in application range.

The application discloses preparation of silver nanocluster functionalized monolithic column and selective solid phase microextraction application thereof. The application takes urea and formaldehyde as matrix monomers, takes glutathione or p-aminobenzenesulfonic acid as functionalization-reaction catalysis bifunctional agent, and prepares glutathione functionalized monolithic column or p-aminobenzenesulfonic acid functionalized monolithic column through one-step polycondensation. Then, the multiple interactions between glutathione or p-aminobenzenesulfonic acid and silver ions are utilized to reduce and fix silver nanoclusters in situ on the surface of the monolithic column, so that the silver nanocluster functionalized monolithic column is prepared. The application provides a new way of combining cluster materials with monolithic columns, and the prepared monolithic column can be used as an in-tube solid phase microextraction stationary phase to realize online selective enrichment and high-sensitivity analysis of trace trans fatty acids in complex samples. The application has the advantages of ingenious process, simple operation, no need of expensive instruments, easiness in popularization and strong specificity of analysis objects.

The invention provides a method for detecting geranyl pyrophosphoric acid in water by headspace solid-phase microextraction / gas chromatography-mass spectrometry, which utilizes the characteristic that geranyl pyrophosphoric acid is easy to decompose into linalool under an acidic condition, adsorbs linalool by headspace solid-phase microextraction, and indirectly quantifies geranyl pyrophosphoric acid in combination with a gas chromatography-mass spectrometry technology. The method is easy to operate, low in instrument cost, high in sensitivity and suitable for detecting geranyl pyrophosphoric acid in water bodies such as source water, the generation risk of 2-methylisoborneol can be pre-judged through the content of geranyl pyrophosphoric acid, and technical support is provided for water supply safety.

The invention discloses a high polymer material radiolysis micromolecule product detection method which comprises the following steps: S1, filling a high polymer material into a solid phase microextraction device to form a negative pressure state; s2, performing gamma ray irradiation on the solid-phase microextraction device; s3, solid-phase microextraction fibers in the solid-phase microextraction device are taken out, and radiolysis micromolecule products adsorbed on the solid-phase microextraction fibers are analyzed through a gas chromatography-mass spectrometer; and S4, analyzing and determining the chemical structure of the radiolysis small molecule product, establishing a quantitative working curve between the peak area and the concentration of the standard product by combining the standard product, and calculating to obtain the content of the radiolysis small molecule product of the high polymer material under the radiation dose. According to the invention, solid-phase microextraction is combined with gas chromatography-mass spectrometry to analyze small molecular substances after high polymer materials such as rubber, resin and the like are irradiated, so that the ray irradiation resistance of the high polymer materials is evaluated; by combining qualitative and quantitative analysis methods, the content of micromolecular products of the high polymer material under different radiation doses can be obtained.

The present invention relates to the field of analytical chemistry, and particularly to a solid-phase microextraction probe based on metal-organic framework SU101, its preparation method, and its application in the trace detection of pharmaceuticals and personal care products in water environment. The solid-phase microextraction probe provided by the present invention has environmentally friendly materials and significantly stronger extraction ability than commercial probes, and has the advantages of low detection limit and quantification limit, wide linear range, good reproducibility, etc. Therefore, it can be used to detect extremely trace amounts of polar drugs in environmental water, especially glucocorticoid drugs.

The invention belongs to the technical field of analysis and detection, and particularly relates to a method for screening volatile and semi-volatile odor markers in raw and auxiliary materials of paper printed matters. The data obtained by the headspace solid-phase microextraction gas chromatography / mass spectrometry is qualitatively analyzed on the basis of a triple confirmation mechanism constructed by a deconvolution method, a matching degree method and a retention index method, and the problem of inaccurate qualitative analysis in a complex matrix is solved. In the semi-quantitative analysis, compounds with different polarities are used as internal standard substances, and a calibration strategy is optimized according to the polarity difference of a target object, so that the reliability of an analysis result is improved. In addition, the components of the odor activity values are subjected to intersection analysis to screen out key odor markers. According to the model, the risk of misjudgment of high-concentration low-threshold substances in a traditional method is avoided by quantifying the contribution degree of a single component to the overall smell.

The invention discloses a bifunctional triazine covalent organic framework material, a solid-phase microextraction probe and application, and the bifunctional triazine covalent organic framework material is prepared from 2, 4, 6-tri (4-formyl-biphenyl-4-yl)-1, 3, 5-triazine, 5 '-(4-amino-3, 5-difluorophenyl)-3, 3', 5, 5 '-tetrafluoro-[1, 1': 3 ', 1'-triphenyl]-4, 4, 6-triazine-1, 3, 5-triazine-1, 3, 5 '-tetrafluoro-[1, 1': 3 ', 1'-triphenyl]-1, 3, 5 '-tetrafluoro-[1, 1': 3 ', 1 ''-triphenyl]-4, 4, 6-triazine-1, 3, 5]- The polymer is formed by polymerizing three monomers, namely 1, 4 ', 4'-diamine and diethyl phosphite. The TFP-COF disclosed by the invention is a porous material containing aromatic rings, fluorine atoms and nitrogen heteroatoms, and has a relatively large conjugated system and a plurality of recognition sites; the hydrophilicity and hydrophobicity of the COFs are adjusted through functionalization of the diethyl phosphite, the hydrophilicity is enhanced, the adsorption performance of the TFP-COF on medium and strong polarity analytes is enhanced, and then high-precision detection of the mycotoxins in a wide polarity range is achieved.

The utility model discloses a multichannel solid-phase microextraction device adaptable to sample bottles of various specifications, which relates to the technical field of solid-phase microextraction, and comprises an instrument body and an adapter, and the top surface of the instrument body is provided with an extraction hole site; the top end of the adapter is provided with a positioning groove matched with the probe handle; the bottom end of the adapter is provided with a mounting groove matched with the bottle cap of the sample bottle, and the adapter is provided with a fixing piece for fixing the bottle cap of the sample bottle; the adapter is provided with a probe channel, and the probe channel extends from the positioning groove to the installation groove. When in use, the height of the small-diameter sample bottle is increased by the adapter, so that the small-diameter sample bottle can be conveniently taken out from the sample bottle, and the instrument body is adaptive to the technical processing of sample bottles of various specifications.

The invention relates to a prediction model for the suitable storage period of cigarette box skins based on the volatile component loss characteristic and application thereof.The construction method comprises the steps that a to-be-detected sample is put into a headspace bottle to be subjected to solid-phase microextraction, and the content of volatile components is obtained through a gas chromatography-mass spectrometry detection method; and calculating the relative retention rate of the volatile component, substituting the relative retention rate into the diffusion empirical equation, and selecting the diffusion empirical equation with the maximum R2 as a prediction model. According to the method, a volatile component loss kinetic model is established for a box skin packaging material with peculiar smell, smell evaluation of the peculiar smell of the box skin is combined, and the optimal storage period of the cigarette box skin material in a specific storage environment before the cigarette box skin material is used on a machine is calculated and predicted through a kinetic equation.

The invention belongs to the field of bacteria identification, and particularly relates to a method for rapidly and nondestructively identifying compound bacteria. The method comprises the following steps: culturing single bacterial colonies, respectively measuring the OD600nm absorbance value of bacterial liquid after the culture is completed, and then diluting the bacterial liquid until the OD600nm is equal to 0.05 to obtain diluted bacterial liquid; adsorbing the diluted bacterial liquid by adopting solid-phase microextraction; after gas chromatography and mass spectrometry detection, principal component analysis and partial least squares discrimination / OPLS-DA analysis are carried out on a bacterial VOCs metabolic spectrum to determine different VOCs of different bacteria, so that different bacteria are discriminated. According to the method, the volatile organic compounds of the bacteria are detected by adopting a solid-phase microextraction-gas phase mass spectrometry technology, and a method for non-destructive detection and rapid identification of four different bacteria and drug-resistant pseudomonas aeruginosa is established by combining a multivariate statistical analysis method, so that the method has the advantages of short detection time, no damage to a sample, high specificity and the like.