This invention is directed to a process for irreversibly binding
nucleic acid to
solid phase and corresponding processes for the utilization thereof.
Nucleic acid is bound to
solid phase matrixes exhibiting sufficient hydrophilicity and electropositivity to irreversibly bind the nucleic acids from a sample. These processes include
nucleic acid (double or single stranded
DNA and
RNA) capture from high volume:low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of
solid phase bound
nucleic acid with
enzyme, hybridization or amplification strategies. The invention, solid phase irreversibly bound nucleic acid, may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for
virus extraction, purification, and solid phase amplification from large volume
plasma specimens.