836 results about "Solid phase extraction" patented technology

The invention discloses a gas positive pressure type solid-phase extraction device and an extraction method, and relates to the technical field of solid-phase extraction devices. A gas positive pressure type solid-phase extraction device comprises a base and further comprises a first supporting column and a second supporting column which are both rotationally installed on the base, an upper rotary disc and a lower rotary disc are longitudinally installed on the first supporting column in a sliding mode, a plurality of placing holes are formed in the upper rotary disc and the lower rotary disc, and the first supporting column and the second supporting column are arranged on the base. An upper bottle body and a lower bottle body are inserted into the two sets of containing holes respectively, an opening and closing part for driving the upper rotary disc and the lower rotary disc to ascend and descend is arranged on the first supporting column, and a switching part for driving the first supporting column and the second supporting column to rotate is arranged on the base. According to the device, multiple steps of extraction can be automatically realized, the device is more convenient, labor-saving and efficient to use, and liquid can pass through the filler more quickly, so that the solid-phase extraction efficiency is improved.

The invention discloses a component detection method suitable for a plant extract, and relates to the technical field of plant extract detection, and the component detection method comprises the following steps: S1, carrying out enrichment treatment on a target component by using two-phase liquid-liquid extraction; s2, performing multi-dimensional attribute mapping and component factor construction by using the initial enrichment liquid; s3, performing correlation feature constraint calculation by using the target component response tensor; s4, performing ideal spectrum construction and response difference analysis by using the to-be-compared spectrum feature set; s5, performing mapping analysis by using ideal and actual spectrum residual signals; s6, performing spectrum correction by using the standard comparison spectrum set; the selectivity of target component enrichment is improved by setting biphasic solvent-liquid-liquid extraction, efficient extraction of target components in a plant extract complex system is achieved, compared with existing single solvent extraction or solid-phase extraction, the method has the advantages that the target components are optimally distributed among liquid phases by regulating and controlling interaction among solvents, and the extraction efficiency is improved. And co-extraction of non-target components is reduced.

The invention discloses a pretreatment method for determining perfluorinated and polyfluoroalkyl compounds in edible oil. The pretreatment method comprises the following steps: (1) extraction: weighing the edible oil, adding an isotope labeled standard substance, centrifugally extracting PFAS (perfluoroalkyl) in a shaking table, adding desalted water to accelerate lipid sedimentation, and performing refrigerated centrifugation to obtain an extracting solution; (2) enriching and purifying, namely enriching and purifying the extracting solution by using a method of combining liquid-liquid extraction and solid-phase extraction, freezing and centrifuging, and collecting supernate after nitrogen blowing to a constant volume. According to the pretreatment method disclosed by the invention, aiming at the characteristic that the edible oil is rich in protein and lipid content, a liquid-liquid extraction and solid-phase extraction combined method is selected to remove impurities such as protein, lipid and pigment, so that separation and purification of PFAS are realized, and the sample requirements of subsequent LC-MS / MS analysis are met. According to the pretreatment method, the sample amount is increased, the final constant volume is reduced, the detection limit of the method is reduced, the sample treatment speed is increased, rapid analysis can be carried out, and the stability is good.

The invention relates to the technical field of preparation of solid-phase microextraction devices, and particularly discloses a pretreatment method and analysis application of a quinolone antibiotic sample, a conjugated microporous polymer (CMP) is successfully prepared by adopting a SonogashiraHagihara cross-coupling reaction, and a cationic conjugated microporous polymer CMP-I is obtained by further carrying out methylation treatment on the CMP by using CH3I. According to the present invention, the cationic CMP-I is adopted as the solid phase extraction adsorption material to construct the solid phase microextraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS / MS) analysis method, and the method has characteristics of high sensitivity and low detection limit in the QA detection aspect, and can meet the accurate determination requirement of the trace QA in meat and water.

The invention discloses a microwave digestion-liquid flash spectrometer strontium isotope rapid detection system for multiple types of samples. The automatic sample injection module automatically identifies, grabs and conveys a sample by means of an image and radio frequency identification technology. The microwave digestion module is provided with a self-adaptive program, temperature and pressure are dynamically regulated and controlled through a PID algorithm according to sample characteristics, and efficient and safe digestion is ensured. The automatic separation and purification module intelligently selects a solid-phase extraction column or an ion exchange resin column to purify a sample by matching spectral analysis with a database. The liquid flash spectrometer detection module accurately collects signals by means of a high-sensitivity element and an algorithm. The data processing and control system adopts a multi-layer perceptron neural network, so that the strontium isotope content can be intelligently judged, and the source of abnormal data can be traced. In addition, the system has the functions of automatic cleaning and maintenance, emergency detection flow and remote monitoring, all the modules are connected through standardized interfaces, and the expansibility is high. The system is high in automation degree and accurate in detection, and an efficient and reliable scheme is provided for strontium isotope detection.

The invention relates to a reagent for detecting amatoxin in blood and a detection method thereof. The detection reagent for the amatoxin in the blood comprises an extraction tube, a dilution tube and a standard gradient solution, the extraction tube contains 1.5 mL of acetonitrile, the dilution tube contains 400 [mu] L of a 1% acetic acid aqueous solution, and the gradient concentrations of the standard gradient solution are 1 ng / mL, 5 ng / mL, 10 ng / mL, 20 ng / mL, 50 ng / mL and 100 ng / mL. The method provided by the invention effectively solves the problem of poor signal stability of amatoxin in a blood sample, and meanwhile, complex steps such as solid-phase extraction do not need to be adopted in the detection process, so that the detection time is saved, and a technical support is provided for emergency monitoring of public health emergencies.

The invention relates to application of an aminated beta-cyclodextrin composite material and a method for simultaneously analyzing endogenous and exogenous organic matters in urine by using the aminated beta-cyclodextrin composite material. According to the method, the cellulose-loaded aminated beta-cyclodextrin composite material is used as a solid-phase extraction agent for the first time, the endogenous markers in the urine are efficiently extracted, and the combined analysis of the endogenous markers and the exogenous markers is realized at the same time. A target compound can be efficiently extracted from a complex urine matrix through an optimized solid-phase extraction process which comprises the steps of activation, sample loading, drying, elution, nitrogen blowing and the like. Then, a liquid chromatography-mass spectrometry technology is utilized for analysis, qualitative and quantitative analysis is performed on 80 target objects in a multi-reaction monitoring (MRM) mode, and the method has good recovery rate (60%-130%) and excellent stability and repeatability.

The invention provides a method for rapidly determining the content of 4, 4-dichlorodihydroxydiphenyl ether in a laundry detergent, and belongs to the technical field of detergent component content detection. According to the method, the content of 4, 4-dichlorodihydroxydiphenyl ether in the laundry detergent is measured by adopting a high performance liquid chromatography. In the determination process, an acetonitrile-water-sodium chloride three-phase mixture is used for removing impurities, and a mixed solution of acetonitrile and toluene is used as an extracting agent for solid-phase elution; a flowing phase A is an acetic acid aqueous solution with the pH value of 3.5-4, and a flowing phase B is acetonitrile. According to the method, the high performance liquid chromatography is combined with the solid phase extraction technology, high-sensitivity and high-accuracy determination can be achieved, a standard curve is established, and the reliability and accuracy of a result are further guaranteed. The method disclosed by the invention is simple to operate, good in stability and repeatability, and capable of accurately and rapidly measuring the content of 4, 4-dichlorodihydroxydiphenyl ether.

The invention relates to the technical field of glucose detection, and discloses a method for detecting glucose in d-ribose fermentation broth, which comprises the following steps: step 1, diluting a d-ribose fermentation broth sample and adjusting the d-ribose fermentation broth sample to a predetermined pH value to form a pretreated sample; 2, removing impurities and concentrating target components through solid-phase extraction to obtain a treated sample; 3, separating the sample by using high performance liquid chromatography, setting a mobile phase, a flow rate and a chromatographic column temperature, and separating to obtain a glucose component; and 4, injecting the separated components into a mass spectrum system on line, and detecting a characteristic ion signal of glucose. The high-sensitivity and accurate detection of glucose in the d-ribose fermentation liquor can be realized by adopting a high-performance liquid chromatography and mass spectrometry combined technology. Through optimized sample pretreatment and mobile phase selection, interference of other carbohydrates on glucose detection is reduced, and accurate determination of glucose concentration is ensured.

The invention provides a method for detecting fluorine compounds in shrimps, which comprises the following steps: S1, sample preparation: removing shells of to-be-detected shrimps, and crushing and homogenizing muscular tissues of the to-be-detected shrimps to obtain a to-be-detected sample; s2, extraction: extracting a sample to be detected, retaining an organic phase, and concentrating to obtain a concentrated solution; then adding water which is 5-15 times of the concentrated solution for dilution to obtain a to-be-purified solution; the extraction solvent is an aqueous solution of an organic solvent; s3, purification: purifying the to-be-purified liquid by using a solid-phase extraction column to obtain a purified liquid; s4, concentration and constant volume: concentrating the purified liquid to be nearly dry, redissolving, and filtering to obtain filtrate; and S5, sample detection: detecting the filtrate by using a liquid chromatograph-mass spectrometer, and carrying out qualitative and quantitative analysis on the fluorine compounds in the prawns by adopting a standard curve method. The method can be used for simultaneously determining the contents of 41 PFASs compounds with different physicochemical properties in shrimps.

The invention relates to a method for detecting perfluorinated compounds in water, which comprises the following steps: carrying out solid-liquid separation on a water sample to prepare a liquid to be detected; carrying out on-line solid phase extraction-liquid chromatography-mass spectrometry detection on the to-be-detected liquid, and calculating the content of the perfluorinated compounds in the to-be-detected liquid by an internal standard method. The detection method is high in automation degree, simple and rapid to operate and capable of accurately detecting the perfluorinated compounds in the water.

The application relates to the field of food detection, and discloses a sample pretreatment device for food safety detection, which comprises a shell, a crushing mechanism, a centrifugal mechanism, a material distribution mechanism, a solid-phase extraction mechanism and a nitrogen blowing mechanism are arranged in the shell; juice is obtained after the food sample is crushed, and the juice is subjected to centrifugal treatment in the centrifugal mechanism; the supernatant after centrifugation is temporarily stored and distributed in the material distribution mechanism; the multiple samples after distribution are subjected to solid-phase extraction in the solid-phase extraction mechanism; and the nitrogen blowing mechanism cooperates with the solid-phase extraction mechanism to complete nitrogen blowing work. Through structural innovation and function integration, the crushing, centrifugal, material distribution, extraction and nitrogen blowing concentration five function modules are integrated in the same equipment, the equipment floor area is reduced, the operation process is optimized, the manual intervention frequency is reduced, and the efficiency and reliability of the food safety detection sample pretreatment are remarkably improved.

The invention relates to a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection method for simultaneously determining 150 stimulant substances in cosmetics, and belongs to the technical field of stimulant detection. According to the detection method, a solid-phase extraction and QuECHERS combined pretreatment technology is adopted, firstly, an acetonitrile ultrasonic extraction mode in a QuECHERS method is adopted, secondly, a matrix is purified in a solid-phase extraction mode, an extraction tube adopts a filter type solid-phase extraction column, namely an Agilent Captiva EMR-Lipid filter column, different from a traditional HLB solid-phase extraction column, the filter column does not need to be activated and balanced, and the detection method is simple and convenient to operate. Gravity self-elution can be achieved in the sample purification process, and only 20% of pure water needs to be added into an extracting solution to achieve the purpose of activating a filtering column; and water can also be used as a dispersing agent and an impurity removing agent at the same time, so that impurities dissolved in water can be adsorbed while the sample is dispersed. The detection method provided by the invention can realize LC-MS / MS determination of 150 stimulant substances in cosmetics under the condition of simultaneously satisfying a pretreatment method for extracting 150 stimulant substances in cosmetics.

The invention discloses a method for determining antibiotics in environmental soil, which comprises the following steps: S1, collecting fresh soil, crushing, sieving with a 0.1 mm-0. 2mm sieve, weighing 3 g-5 g of sample, and placing in a centrifuge tube; s2, adding 50-80 mL of a methanol-acetic acid-aqueous solution into the centrifuge tube, oscillating for 3-5 min, then carrying out normal-temperature ultrasonic treatment for 25-30 min, carrying out high-speed centrifugation for 10-15 min at a low temperature of 4-6 DEG C, taking a supernatant, repeatedly extracting residues twice, merging the obtained extract and the supernatant, and concentrating to 10-15 mL to obtain a concentrated solution A; s3, adding 20-25 mL of dichloromethane into the concentrated solution, repeating the extraction operation in the S2 to obtain a concentrated solution B, and adding ultrapure water into the concentrated solution B to dilute the concentrated solution B to 200 mL; and S4, adding 1-1.5 g of disodium ethylene diamine tetraacetate into the diluent obtained in S3, adjusting the pH value to 3.5, enabling the diluent to pass through a small solid-phase extraction column, eluting the small solid-phase extraction column with methanol, blow-drying under nitrogen flow, fixing the volume, passing through a filter membrane, and transferring into a sample bottle for UPLC-MS / MS determination. According to the method, the antibiotic extraction rate and the content determination accuracy are improved.

The invention discloses a high-throughput screening method for broad-spectrum new pollutants, which comprises the following steps: pre-treating a sample to be detected by using a mixed extracting agent of methanol and dichloromethane and an HLB solid-phase extraction small column, extracting the broad-spectrum new pollutants, and detecting the broad-spectrum new pollutants by using an ultra-high performance liquid chromatography-high resolution mass spectrometry technology. And carrying out data acquisition on the extracting solution in a full-scanning and full-ion fragmentation scanning mode. And then, the collected high-resolution mass spectrum data is compared with a self-established suspected target screening database containing N pollutants, and the data is integrated and analyzed according to a set screening and filtering standard, so that efficient and rapid matching and identification of the N pollutants in the sample are realized. According to the detection method, the to-be-detected sample can still be rapidly screened in the suspected target screening database under the condition that no standard substance exists, the detection speed and efficiency are remarkably improved, and meanwhile the detection cost is reduced.

The solid-phase extraction device with the cleaning structure comprises a base, a supporting frame is arranged at the top end of the base, and a lifting plate is connected into the supporting frame in a sliding mode; a moving mechanism is arranged at the top end of the base, and an adjusting assembly is arranged on one side of the supporting frame. Wherein the moving mechanism comprises a moving plate slidably connected to the interior of the top end of the base, supporting plates are symmetrically installed on the two sides of the top end of the moving plate, supports are symmetrically slidably connected to the interior of the top end of the moving plate, fixing seats are symmetrically installed at the top end of the base, and rotating plates are rotatably connected to the interiors of the fixing seats; therefore, the effect of rapidly adjusting and switching the collecting pipe can be achieved, the effect of cleaning waste liquid while solid-phase extraction in the liquid can be achieved, the solid-phase extraction efficiency in the liquid is greatly improved, the cleaning speed of the collecting pipe can be further improved, and the phenomenon that the use efficiency of the device is affected due to operation conflict of the two is avoided.

The present invention provides a kit and a detection method for synchronously detecting catecholamines and their metabolites, which relates to the technical field of analytical detection. The kit contains a diluent, a solid-phase extraction reagent, an LC-MS / MS detection reagent, a solid-phase extraction device, and a solid-phase extraction packing material. Among them, the solid-phase extraction packing material creatively adopts zwitterionic exchange microspheres containing both carboxyl and quaternary ammonium groups. The kit can not only synchronously and efficiently separate and detect 8 kinds of catecholamines and their metabolites in urine samples, but also separate and detect 6 kinds of amino-containing catecholamines and their metabolites in blood samples. When the above kit is used to separate and detect catecholamines and their metabolites, the extraction recovery rate is relatively high, and the linear range covers the reference value range of normal detection, which can significantly shorten the detection time of urine samples, improve the detection efficiency of mass spectrometry, and is conducive to popularization and application in clinical detection.

The application provides a pretreatment and detection method of tetrabromobisphenol A and ten common derivatives thereof in sludge, which comprises the following steps: (1) sample pretreatment: after mixing the frozen and dried sludge sample and the isotopic internal standard, oscillation extraction is carried out, and the extraction liquid is obtained after centrifugation by a centrifuge; the extraction liquid is purified by solid phase extraction to obtain a sample to be detected; (2) the sample to be detected obtained in the step (1) is detected by high performance liquid chromatography-tandem mass spectrometry; (3) the content of tetrabromobisphenol A and ten common derivatives thereof in the sample is calculated according to the internal standard method. The application provides a rapid and sensitive analysis and quantitative method of tetrabromobisphenol A and ten common derivatives thereof in sludge samples.

The invention discloses a method for detecting multiple perfluorinated compounds in a water environment. The method comprises the steps of sample collection and preservation, sample pretreatment and LC-MS / MS detection. According to the invention, trace detection is carried out on common perfluorinated compounds in a water environment by adopting solid-phase extraction-liquid chromatography-electrospray-tandem triple quadrupole mass spectrometer, a more perfect and comprehensive LC-MS / MS detection method for multi-medium concentration of perfluorinated compounds is provided, and on the premise that the recovery rate and the accuracy of a detection result are ensured, the detection efficiency is improved. The sample pretreatment modes in different media are optimized and adjusted, so that the experiment process is more convenient and faster, time and labor are saved, the use of traditional glass material consumables is avoided, and the experiment error is reduced; meanwhile, the method disclosed by the invention is wider in application range, can meet trace detection of perfluorinated compounds in surface water, underground water, domestic sewage, industrial wastewater, river sediment and residual sludge of a sewage plant, and provides data support for quality supervision and risk early warning in a water environment.

The invention belongs to the technical field of antibiotic detection, and particularly discloses a synchronous detection method for 42 antibiotics in estuary multi-environment media. The method comprises the following steps: mixing an estuary sample to be detected, a phosphate buffer solution and a methanol-acetonitrile solution of formic acid, carrying out multiple times of ultrasonic extraction, combining extract liquids obtained by the multiple times of ultrasonic extraction, sequentially carrying out pH value regulation and concentration, and then adding water for dilution, so as to obtain an extraction diluent; then carrying out purification treatment on the extraction diluent by adopting a solid-phase extraction method to obtain a to-be-detected solution; adding five antibiotic internal standards into the to-be-detected solution, fixing the volume, detecting the to-be-detected solution by adopting an ultra-high performance liquid chromatography tandem mass spectrometer, and performing data processing on a detection result to obtain the concentration of 42 antibiotics in the to-be-detected estuary sample. The test method disclosed by the invention can be used for trace detection of antibiotics in a complex environmental medium in an estuary region, has the characteristics of low detection limit, good reproducibility and relatively high recovery rate, and can meet analysis requirements.

The invention discloses a novel high performance liquid chromatography-tandem mass spectrometry analysis method for detecting aspartatide in human plasma, teriparatide is used as an internal standard substance to be detected, a solid phase extraction method is adopted to extract aspartatide to be detected and internal standard teriparatide from a plasma sample, and a standard curve is linear in a range of 0.010-1.00 ng / mL. During the verification of the method, selectivity, matrix effect, recovery, sensitivity, linearity, accuracy and precision, stability and dilution reliability are assessed to meet standards. The method disclosed by the invention has specificity on determination of the to-be-detected substance, i.e., the aspartatide and the internal standard teriparatide in the human plasma, and a standard curve is linear in a range of 0.010-1.00 ng / mL. The quantitative lower limit of methodology is 0.01 ng / mL. The method has the advantages of good accuracy, reliability, high sensitivity, high specificity and reproducibility.

The invention discloses a method for separating and enriching 24 perfluorinated compounds in a water environment. The method comprises the following steps: collecting a to-be-detected water sample, filtering, and adding an isotope labeled recovery rate indicator to obtain a pretreated water sample; activating a PWAX solid-phase extraction column by using a 0.1% ammonia water methanol solution, methanol and ultrapure water in sequence, and enriching a water sample through the column; leaching the PWAX solid-phase extraction column with a 25 mM ammonium acetate aqueous solution (pH = 4) to remove water-soluble interference impurities; carrying out vacuum freeze drying on the solid-phase extraction column, and eluting with methanol and 0.1% ammonia water methanol solution; and blowing the collected eluent to 200 L under nitrogen flow, adding 1: 1 methanol and aqueous solution into the obtained residue, redissolving to 1.00 mL, filtering with a filter membrane, and putting into a polypropylene sample injection bottle to complete separation and enrichment. The method disclosed by the invention is simple and convenient to operate, can enrich various types of perfluorinated compounds (including carboxylic acids, sulfonic acids, fluorosulfonic acids and the like) in water, and is wide in application range and relatively high in accuracy.

The invention relates to the technical field of analytical chemistry, in particular to a method for simultaneously determining seven fat-soluble vitamin compounds. The method comprises the following steps: in 0-4.5 minutes, transferring a sample solution into an on-line solid-phase extraction column by a sample loading pump, and communicating an analysis pump with a liquid chromatographic analysis column through a switching valve for balancing; in 4.5-6.5 min, adjusting the state of a switching valve, respectively communicating two ends of the online solid-phase extraction column with an analysis column and an analysis pump of the liquid chromatography, and backflushing the analyte from the online solid-phase extraction column and transferring the analyte into the analysis column of the liquid chromatography by a mobile phase of the analysis pump; and 6.5-42 min, adjusting the state of the switching valve, flushing and balancing the on-line solid phase extraction column by the sample loading pump, and completing separation and analysis of the analyte by matching the analysis pump with the analysis column of the liquid chromatography. According to the method disclosed by the invention, saponification liquid can be directly analyzed on a machine, and seven target compounds can be separated and detected within 42 minutes.

The invention provides a kit for synchronously detecting catecholamine and metabolites thereof and a detection method, and relates to the technical field of analysis and detection. The kit comprises a diluent, a solid-phase extraction reagent, an LC-MS / MS detection reagent, a solid-phase extraction device and a solid-phase extraction filler. Wherein the solid-phase extraction filler creatively adopts an amphiphilic ion exchange microsphere containing two functional groups, namely carboxyl and quaternary ammonium at the same time. The kit can be used for synchronously and efficiently separating and detecting eight catecholamines and metabolites thereof in a urine sample; and 6 kinds of catecholamines containing amino groups and metabolites thereof in a blood sample can be separated and detected. When the kit is used for separating and detecting catecholamine and metabolites thereof, the extraction and recovery rate is high, the linear range covers the reference value range of normal detection, the detection time of a urine sample can be remarkably shortened, the detection efficiency of mass spectrum is improved, and popularization and application in clinical detection are facilitated.

The utility model discloses a solid phase extraction column passing device which comprises a shell, the shell is fixedly connected with a first transverse plate and a second transverse plate, a plurality of stand columns are fixedly connected between the first transverse plate and the shell, moving plates are arranged on the stand columns in a sliding mode, a driving mechanism is arranged on the shell, and a plurality of first through holes are formed in the first transverse plate. A plurality of second through holes are formed in the second transverse plate, solid-phase extraction columns are placed in the first through holes and the second through holes, a plurality of rubber plugs are fixedly connected to the movable plate, and connecting pipes are fixedly connected to the interiors of the rubber plugs; the auxiliary assembly comprises a liquid conveying mechanism and a pressurizing mechanism, the liquid conveying mechanism is arranged on the shell and provided with a plurality of first hoses communicated with the connecting pipe, and the pressurizing mechanism is provided with a plurality of second hoses communicated with the connecting pipe. In the solid-phase extraction process, the manual operation steps can be reduced, so that the operation process is more convenient, the operation time of workers is reduced, and the use is more convenient.

The invention discloses a method for detecting TTX and 4, 9-anhydroTTX in shellfish through ion pair solid phase extraction column purification-liquid chromatography-tandem mass spectrometry, and the method comprises the following steps: (1) pretreatment; (2) extracting; (3) enriching and purifying; and (4) loading a sample and detecting. The method for detecting TTX and 4, 9-anhydroTTX in shellfish through ion pair solid phase extraction column purification-liquid chromatography-tandem mass spectrometry is established by optimizing extraction, enrichment purification and chromatographic conditions, the method can be used for detecting TTX and 4, 9-anhydroTTX in shellfish samples at the same time, operation is easy and convenient, accuracy, precision and reproducibility are good, and the method can be suitable for quantitative analysis of TTX in shellfish and 4, 9-anhydroTTX homologues of TTX in shellfish.

The invention relates to the technical field of food safety detection, in particular to solid-phase extraction filler for detecting pesticide residues in food and a preparation method and application thereof.The solid-phase extraction filler comprises a magnetic filler core and a selective adsorption layer wrapping the magnetic filler core; wherein the interior of the magnetic filler core at least comprises magnetic particles and a coating layer for improving the stability of the magnetic particles; the selective adsorption layer is composed of a porous organic framework material which is chemically grafted outside the coating layer; and a temperature-sensitive polymer gel layer also covers the outer part of the selective adsorption layer. According to the solid-phase extraction filler disclosed by the invention, through an innovative hierarchical structure design, the extraction of trace pesticide residues in food can be effectively improved, the extraction difficulty is reduced, and the extraction efficiency and the extraction selectivity are improved.

The invention relates to the technical field of detection of organic nitrate, in particular to a method for detecting granular organic nitrate in soil. The method comprises the following steps: collecting a representative soil sample, labeling information, properly storing, carrying out freeze drying treatment on the sample, removing moisture, grinding the dried sample into fine powder, sieving to remove impurities, extracting the sample, obtaining a target compound, further purifying an extracting solution through a solid-phase extraction technology, removing interferents, and obtaining the target compound. And concentrating the purified solution to a proper volume by using a nitrogen blowing concentrator, redissolving the concentrated solution, fixing the volume to an accurate volume, and detecting the organic nitrate in the sample after the volume is fixed. The detection sensitivity of the organic nitrate is improved, a standard curve is established by adopting standard substance preparation and detection, the concentration of the organic nitrate in a sample is calculated by combining an external standard method, and the accuracy of a detection result is ensured.

Methods and devices for the washing, extraction, and separation of a sample in a disposable chromatography cartridge (201) comprising a barrel (204) and a column (205), and especially including reinforcement to the column permitting high-pressure separation.

The invention relates to the field of drug analysis, and discloses a trace analysis method and system for antiviral drugs in sewage based on LC-MS / MS. The method comprises the following steps: filtering a sewage sample, analyzing the drug properties of the antiviral drugs, carrying out solid-phase extraction on the filtered sewage sample, preparing an LC-MS / MS module of the extracted sample, determining a mobile phase of a chromatographic column, and determining the mobile phase of the chromatographic column. Balancing the chromatographic column, constructing chromatographic conditions for balancing the chromatographic column, determining a tuning solution and a mass axis calibration compound of a mass spectrometer, calibrating the mass spectrometer, and constructing mass spectrum conditions of the calibrated mass spectrometer; the method comprises the following steps: preparing a standard solution containing an antiviral drug, analyzing the standard solution, constructing a standard curve of the standard solution, and calculating a response coefficient of an LC-MS / MS module; and analyzing the extracted sample to obtain a sample mass spectrum and a sample chromatogram, and calculating the content of the antiviral drug. According to the method, the sensitivity and accuracy of trace analysis of the antiviral drugs in the sewage can be improved.