429 results about "Solid phase extraction" patented technology

The invention provides a method for detecting fluorine compounds in shrimps, which comprises the following steps: S1, sample preparation: removing shells of to-be-detected shrimps, and crushing and homogenizing muscular tissues of the to-be-detected shrimps to obtain a to-be-detected sample; s2, extraction: extracting a sample to be detected, retaining an organic phase, and concentrating to obtain a concentrated solution; then adding water which is 5-15 times of the concentrated solution for dilution to obtain a to-be-purified solution; the extraction solvent is an aqueous solution of an organic solvent; s3, purification: purifying the to-be-purified liquid by using a solid-phase extraction column to obtain a purified liquid; s4, concentration and constant volume: concentrating the purified liquid to be nearly dry, redissolving, and filtering to obtain filtrate; and S5, sample detection: detecting the filtrate by using a liquid chromatograph-mass spectrometer, and carrying out qualitative and quantitative analysis on the fluorine compounds in the prawns by adopting a standard curve method. The method can be used for simultaneously determining the contents of 41 PFASs compounds with different physicochemical properties in shrimps.

The application relates to the field of food detection, and discloses a sample pretreatment device for food safety detection, which comprises a shell, a crushing mechanism, a centrifugal mechanism, a material distribution mechanism, a solid-phase extraction mechanism and a nitrogen blowing mechanism are arranged in the shell; juice is obtained after the food sample is crushed, and the juice is subjected to centrifugal treatment in the centrifugal mechanism; the supernatant after centrifugation is temporarily stored and distributed in the material distribution mechanism; the multiple samples after distribution are subjected to solid-phase extraction in the solid-phase extraction mechanism; and the nitrogen blowing mechanism cooperates with the solid-phase extraction mechanism to complete nitrogen blowing work. Through structural innovation and function integration, the crushing, centrifugal, material distribution, extraction and nitrogen blowing concentration five function modules are integrated in the same equipment, the equipment floor area is reduced, the operation process is optimized, the manual intervention frequency is reduced, and the efficiency and reliability of the food safety detection sample pretreatment are remarkably improved.

The solid-phase extraction device with the cleaning structure comprises a base, a supporting frame is arranged at the top end of the base, and a lifting plate is connected into the supporting frame in a sliding mode; a moving mechanism is arranged at the top end of the base, and an adjusting assembly is arranged on one side of the supporting frame. Wherein the moving mechanism comprises a moving plate slidably connected to the interior of the top end of the base, supporting plates are symmetrically installed on the two sides of the top end of the moving plate, supports are symmetrically slidably connected to the interior of the top end of the moving plate, fixing seats are symmetrically installed at the top end of the base, and rotating plates are rotatably connected to the interiors of the fixing seats; therefore, the effect of rapidly adjusting and switching the collecting pipe can be achieved, the effect of cleaning waste liquid while solid-phase extraction in the liquid can be achieved, the solid-phase extraction efficiency in the liquid is greatly improved, the cleaning speed of the collecting pipe can be further improved, and the phenomenon that the use efficiency of the device is affected due to operation conflict of the two is avoided.

The application provides a pretreatment and detection method of tetrabromobisphenol A and ten common derivatives thereof in sludge, which comprises the following steps: (1) sample pretreatment: after mixing the frozen and dried sludge sample and the isotopic internal standard, oscillation extraction is carried out, and the extraction liquid is obtained after centrifugation by a centrifuge; the extraction liquid is purified by solid phase extraction to obtain a sample to be detected; (2) the sample to be detected obtained in the step (1) is detected by high performance liquid chromatography-tandem mass spectrometry; (3) the content of tetrabromobisphenol A and ten common derivatives thereof in the sample is calculated according to the internal standard method. The application provides a rapid and sensitive analysis and quantitative method of tetrabromobisphenol A and ten common derivatives thereof in sludge samples.

The invention relates to the technical field of food safety detection, in particular to solid-phase extraction filler for detecting pesticide residues in food and a preparation method and application thereof.The solid-phase extraction filler comprises a magnetic filler core and a selective adsorption layer wrapping the magnetic filler core; wherein the interior of the magnetic filler core at least comprises magnetic particles and a coating layer for improving the stability of the magnetic particles; the selective adsorption layer is composed of a porous organic framework material which is chemically grafted outside the coating layer; and a temperature-sensitive polymer gel layer also covers the outer part of the selective adsorption layer. According to the solid-phase extraction filler disclosed by the invention, through an innovative hierarchical structure design, the extraction of trace pesticide residues in food can be effectively improved, the extraction difficulty is reduced, and the extraction efficiency and the extraction selectivity are improved.

The invention belongs to the field of soil dissolved organic matter analysis, and provides a multispectral-based DOM detection and reclamation influence evaluation method, which comprises the following steps: step 1, collecting dry farmland soil samples of different reclamation years, and obtaining soil column samples of a plough layer, a plow pan and subsoil; 2, extracting, centrifuging and filtering the soil sample to obtain a DOM solution; 3, detecting the DOM solution by adopting an ultraviolet-visible absorption spectrum; step 4, adopting a three-dimensional fluorescence spectrum and combining parallel factor analysis to obtain fluorescence components and source characteristics of the DOM solution; step 5, analyzing molecular composition and characteristic parameters of the DOM solution by adopting solid-phase extraction in combination with Fourier transform ion cyclotron resonance mass spectrometry; and step 6, based on the detection data in the steps 3-5, evaluating the influence of reclamation years on the composition, structure and degradation characteristics of the DOM. According to the method, multi-dimensional detection of soil DOM is realized through a multi-spectrum and mass spectrum combined technology, and DOM is comprehensively analyzed from a macrostructure to molecular composition.

The present invention relates to a method for detecting and / or quantifying an analyte in a sample using mass spectrometry. The method of the invention comprises: extracting the analyte from the sample using solid phase extraction (SPE) so as to obtain an SPE extract comprising the analyte, concentrating the analyte, said concentrating comprising evaporating solvent from the SPE-extract; and detecting and / or quantifying the analyte in the sample using mass spectrometry.

The invention relates to the technical field of environmental analysis and testing, and discloses a method for detecting perfluorinated compounds in soil, which comprises the following steps: (1) crushing and sieving a soil sample to be detected, then carrying out pressurized fluid extraction, carrying out rotary evaporation concentration on the obtained extract, and dissolving with methanol again to obtain a concentrated solution; (2) purifying the concentrated solution through a solid-phase extraction column, and then concentrating to a constant volume to obtain a to-be-detected solution; (3) detecting the type and the content of the perfluorinated compounds in the to-be-detected liquid by adopting a liquid chromatography-tandem mass spectrometry method; wherein in the step (1), an extracting solvent is a NaOH methanol solution; in the step (3), in the liquid chromatography-tandem mass spectrometry, the liquid chromatographic condition is as follows: a liquid chromatographic column is a C18 chromatographic column. The detection method provided by the invention is simple to operate, high in accuracy, good in repeatability, capable of analyzing multiple PFAS types (17 types) and low in detection limit, meets the analysis requirements, and can be used for analyzing and monitoring common PFAS in soil.

This invention discloses a pretreatment method for detecting trace perfluorinated and polyfluoroalkyl substances (PFAS) in complex matrix textiles, belonging to the field of materials analysis technology. The method involves placing the textile in an alkaline solvent containing an alkyl sulfonate displacing agent. Under heating conditions, the displacing agent utilizes its competitive adsorption mechanism to forcibly occupy matrix adsorption sites and displace the target analytes into the solvent. Subsequently, the temperature is rapidly lowered to the phase separation induction temperature, inducing supersaturation precipitation of the displacing agent, which then encapsulates impurities in situ and co-precipitates, forming a solid-liquid stratified system. Finally, the supernatant is collected for analysis. This invention utilizes the dual properties of the displacing agent—high-temperature solubilization and low-temperature phase change removal—to effectively break the strong adsorption equilibrium of aged textiles without using a solid-phase extraction column. This solves the contradiction between low extraction rate and significant matrix interference, significantly improving the recovery rate of PFAS. It offers advantages such as low cost, simple operation, and high accuracy.

This invention relates to a system flow path for solid-phase extraction and its application method. The system flow path is characterized by a pump inlet port located below a rotary valve, several solvent inlet ports on the circumference of the rotary valve, a solvent outlet port and a waste liquid port on the circumference of the rotary valve, a gas path system connecting the solvent outlet port of the rotary valve to the solvent interface of the sample loader, and a sample loader comprising a valve core and a valve body. The top of the valve body is connected to a sample vial, and a spray needle is also located at the top of the valve body. The valve core is located within the valve body. The sample flow channel is connected to a guide tube, and a membrane adapter is located below the guide tube. The membrane adapter is connected to a collection docking point, which is connected to a dehydration device and a collection vial. The collection docking point is also connected to a flow path switching system. This invention features a compact structure, anti-clogging and anti-cross-contamination advantages, and spray cleaning of the sample vial ensures complete sample loading, improving experimental recovery rates. The online filtration and dehydration functions enable full-process automation.

The application relates to the technical field of testing devices, and specifically discloses a solid-phase extraction device which comprises a solid-phase extraction instrument box body and a top cover, the inner cavity of the top end of the solid-phase extraction instrument box body is clamped with the top cover, the bottom end of the solid-phase extraction instrument box body is fixedly connected with a support, and the inner cavity of the support is placed with a test tube. The buffer assembly can give an alarm during the buffering process, and when the abnormal pressure reaches the shielding value, the alarm can be given to remind the staff to maintain, the alarm can be given when the pressure fluctuation drives the support to shake, the staff can find in time, the risk of liquid leakage caused by test tube deformation is prevented, the dosing assembly can quantitatively dose multiple groups of samples, the amount can be adjusted according to requirements, the accuracy in the material transfer process can be ensured, in addition, the test samples remaining on the inner wall of the dosing instrument pipeline can all fall off, meanwhile, the splashing of the extraction liquid in the falling process is prevented, and therefore the accuracy of experimental data is improved.

The invention provides equipment and method for detecting new pollutants and a detection system. The equipment comprises a sample injection module, a multi-channel switching module, a solid-phase extraction module, a collection module, a first control module, an immunoassay sensor and a power module, and the sample injection module comprises a detection sample storage unit, an eluent storage unit and a cleaning liquid storage unit; the multi-channel switching module is at least provided with a first port, a second port, a third port and a fourth port, the fourth port is communicated with one of the first port and the second port in a switchable manner, the third port is communicated with one of the fourth port and the first port in a switchable manner, the first port is connected with the detection sample storage unit, the second port is connected with the eluent storage unit, and the eluent storage unit is connected with the detection sample storage unit. The third port is connected with the cleaning liquid storage unit; the solid-phase extraction module is provided with a feed port and a discharge port, the feed port is connected with the fourth port, and a constant volume unit is arranged at the discharge port; the first control module is connected with the detection sample storage unit, the eluent storage unit, the cleaning liquid storage unit and the multi-channel switching module.

The application belongs to the technical field of material application, and particularly relates to a graphitized carbon material and a preparation method and application thereof, the preparation method of the graphitized carbon material is as follows: (1) D-(+)-xylose powder and transition metal salt powder are weighed and uniformly ground; (2) after sieving, calcination is carried out under an inert gas atmosphere; (3) after grinding and sieving of the calcined product, washing is carried out with 1.0 mol / L dilute hydrochloric acid solution, then washing is carried out with distilled water and drying is carried out, to obtain the graphitized carbon material, the graphitized carbon material has high adsorption efficiency on aflatoxin B1 (AFB1) in vegetable oil, and in combination with magnetic solid phase extraction (MSPE) and high performance liquid chromatography-fluorescence detection (HPLC-FLD) technology, the detection limit is as low as 2.0 pg·g ‑1 The graphitized carbon material has excellent regeneration performance, and the material consumption and cost in the detection process are significantly reduced.

The application relates to the technical field of isotope separation, in particular to a SiO2@CeO2 core-shell material with silica supported cerium dioxide, a preparation method thereof and application of the SiO2@CeO2 core-shell material in Ge / Ga separation. 68 Efficient adsorption of Ge and 68 Effective elution of Ga; the SiO2@CeO2 core-shell material is used as a stationary phase, hydrochloric acid is used as a mobile phase, Ge / Ga is efficiently separated based on solid-phase extraction, and therefore the SiO2@CeO2 core-shell material can be used as 68 Ge- 68 Ga generator column packing.

The application provides a method for detecting psychotropic drugs in environmental sludge based on acid pretreatment, which comprises the following steps: S1: obtaining a sludge sample to be detected, performing pressurized fluid extraction on the sludge sample to be detected after freeze-drying to obtain an extraction solution, performing solid-phase extraction on the extraction solution to obtain an eluate, and redissolving the eluate to obtain a solution to be detected, wherein the pressurized fluid extraction is performed under the condition of 5 ‰ formic acid water without heating; and S2: performing liquid chromatography mass spectrometry on the solution to be detected to obtain a mass spectrum, wherein the psychotropic drug is one of eptazocine, codeine, AMP, MAMP, MDA, monoacetylmorphine, MDMA, PMMA, fentanyl, norfentanyl, ketamine, norfentanyl, benzoyl eptazocine, cocaine and tramadol, and the method realizes detection of multiple psychotropic drugs in environmental sludge.

The present application relates to the technical field of oil and gas exploration, and particularly relates to a method for quantitatively detecting adamantane compounds in rock. The method comprises the following steps: crushing a rock sample at low temperature, taking the crushed rock sample into a container, adding diatomite, a standard sample solution and an extractant, and sealing the container; performing ultrasonic extraction and low-temperature standing; centrifugally collecting supernatant, and placing the supernatant in a fume hood for volatilization; when 1-2 mL of supernatant remains, adding alumina for adsorption; before the solvent is completely volatilized, taking out the adsorbed alumina, and performing solid-phase extraction; concentrating the eluent after solid-phase extraction, and performing gas chromatography-mass spectrometry analysis on the concentrated solution. The method can quantitatively detect adamantane compounds in rock, has high accuracy, and simple operation steps.

The invention relates to the technical field of analysis and detection, in particular to a method for detecting zeranol in a meat sample. The detection method comprises the following steps: pretreating a meat sample by adopting a magnetic dispersive solid-phase extraction method, and then detecting zeranol by adopting an immunochromatography technology, the magnetic dispersion solid-phase extraction method adopts an HLB magnetic nano material. The detection method provided by the invention has good accuracy, sensitivity and specificity, and can meet the requirement of rapid detection of zeranol in meat samples. The method is simple and convenient to operate, does not need professional detection equipment, can be operated by common law enforcement officers or food supervisors through simple training, and has a wide application prospect.

The invention discloses a full-automatic solid-phase extraction instrument which comprises a main machine shell and a main machine in the main machine shell, a plunger module, a test tube rack module and a concentration constant volume module in the main machine are mounted on a rack provided with various axial movement modules for continuous operation of extraction and concentration; wherein in each group of solid-phase extraction assembly in the plunger module, a solution is injected and added into the SPE column through a plunger rod; a plurality of test tubes corresponding to the solid-phase extraction assembly are arranged in the test tube rack module so as to receive corresponding filtrate; in the concentration constant volume module, corresponding elution sample liquid is received through a constant volume pipe, air blowing and liquid adding are performed by an air blowing and liquid adding assembly arranged on one side of the constant volume pipe, and then constant volume is performed on the elution sample liquid by sensing the liquid level height in the constant volume pipe through a constant volume module arranged at the bottom of the constant volume pipe. According to the invention, the structure of each module in the solid-phase extraction process is more compact, and the adaptability of the whole instrument to different samples is improved.

The invention provides a method for detecting sulfonamides in animal-derived food through capillary electrophoresis-laser-induced fluorescence, and belongs to the technical field of detection. Comprising the following steps: (1) mincing a sample, adding an extracting solution for extraction, and carrying out solid-phase extraction column extraction and methanol elution to obtain an eluted sample solution; (2) preparing a sulfanilamide drug standard substance and fluorescamine into gradient solutions by using methanol and acetonitrile respectively, and stirring at room temperature for reaction to obtain a solution A; (3) mixing the elution sample solution with a fluorescent amine-acetonitrile solution, and stirring and reacting at room temperature to obtain a solution B; and (4) detecting the fluorescence intensity of the sulfonamides in the solution A by adopting capillary electrophoresis-laser induced fluorescence to obtain a linear equation for detecting the concentration of the sulfonamides in the solution B. The invention establishes a novel method for detecting sulfonamide residues in animal-derived food by using high-efficiency capillary electrophoresis-laser-induced fluorescence, and realizes high-sensitivity detection of three sulfonamides in animal-derived food.

The invention relates to a method for detecting the content of lutein and / or zeaxanthine, which comprises the following steps: taking a standard substance of a sample to be detected, and preparing a plurality of groups of standard substance solutions with different concentrations; testing the standard substance solution by adopting an online solid-phase extraction-liquid chromatography to obtain a standard working curve; pretreating a sample to be detected to obtain a sample solution, testing the sample solution by adopting an online solid-phase extraction-liquid chromatography, and substituting the obtained result into the standard working curve to obtain the content of xanthophyll and / or zeaxanthine. According to the method provided by the invention, the separation effect between the xanthophyll and the isomer thereof is improved, the content of the xanthophyll and the isomer thereof can be accurately measured, the repeatability is good, the accuracy is high, the overall analysis time is short, and the sample analysis and detection efficiency is remarkably improved.

The invention discloses a method suitable for on-site analysis of the valence state of thallium in water. The method comprises the following operation steps: step 1, collecting and separating a water sample; step 2, acidizing treatment; 3, Tl (I) and Tl (III) are separated; 4, total thallium is measured; step 5, Tl (I) determination; the invention relates to the technical field of atomic spectrum metal element analysis, and the method has the beneficial effects that by combining an electrochemical separation technology with a Chelex-100 solid-phase extraction column and cooperating with a portable liquid cathode glow discharge thallium measurement instrument, high-sensitivity field detection of Tl (I) and Tl (III) is realized, the detection limit is lower than 1.0 g / L, and the repeatability is RSDlt; the analysis period of a single sample is less than or equal to 30 minutes, the weight of equipment is only 5kg, battery power supply is supported, and acidification-separation-detection integrated operation can be completed outdoors without professional laboratory conditions.

The invention belongs to the technical field of environmental analytical chemistry, and particularly relates to a separation and purification and high-throughput target quantitative analysis method for multiple types of environmental pollutants in a complex matrix biological sample. The method comprises the following specific steps: S1, carrying out ultrasonic extraction on a biological sample to obtain an extracting solution; s2, performing dispersive solid-phase extraction and purification treatment on the extracting solution to obtain a solution to be detected; s3, determining the environmental pollutants in the liquid to be detected by adopting a selected ion scanning mode of the gas chromatograph-mass spectrometer. The pretreatment method disclosed by the invention can effectively reduce complex matrix interference of the biological sample, and can quickly and efficiently perform high-throughput target analysis on multiple types of environmental pollutants in the biological sample; the method disclosed by the invention is low in detection limit and capable of realizing trace detection of a biological sample; the invention provides a new method for researching the occurrence condition of the environmental pollutants in the 17 marine organisms in Liaoning Bay, and has important significance for the progress of environmental science.

This invention relates to the field of high-purity material analysis technology, and discloses a method for simultaneously separating and enriching copper, cadmium, nickel, cobalt, zinc, lead, and barium in lithium carbonate. The steps are as follows: dissolving a lithium carbonate sample in nitric acid to obtain an acidified sample solution; adjusting the acidified sample solution to an alkaline pH range of 9.5-10.5 using an ammonia-ammonium acetate alkaline buffer solution; passing the alkaline solution through a solid-phase extraction column filled with iminodiacetic acid-type chelating resin, where copper, cadmium, nickel, cobalt, zinc, lead, and barium ions are selectively adsorbed onto the resin; and eluting the adsorbed heavy metal ions with acid to achieve separation and enrichment. This invention effectively overcomes the inhibition and interference of the lithium carbonate matrix on the detection of trace heavy metals, and in particular significantly improves the adsorption and recovery rate of elements such as barium. It achieves efficient separation and enrichment of multiple heavy metals in complex matrices, providing a reliable pretreatment solution for high-sensitivity and high-accuracy quality control analysis of lithium carbonate products.

The embodiment of the present application provides a positive pressure column passing device, which comprises a seat body, a hole plate and a positive pressure assembly. The positive pressure assembly has a gas inlet and a plurality of gas inlet units. The gas inlet unit comprises a gas outlet, a gas inlet cavity and a gas outlet cavity. The gas inlet cavity is communicated with the gas inlet, and the gas outlet cavity is communicated with the gas outlet. The gas outlet corresponds to the mounting hole, so as to be used for the gas to enter the solid phase extraction column. The gas inlet unit has a communication state and a separation state. The gas inlet unit is abutted with the solid phase extraction column, so that the gas inlet cavity and the gas outlet cavity are communicated, and a gas flow path passing through the gas inlet, the gas inlet cavity, the gas outlet cavity, the gas outlet and the solid phase extraction column in sequence is formed. The gas inlet unit is in the communication state. When the gas inlet unit is separated from the solid phase extraction column, the gas inlet cavity and the gas outlet cavity are separated to cut off the gas flow path, and the gas inlet unit is in the separation state. The positive pressure column passing device of the embodiment of the present application can reduce the risk of gas leakage.

The invention provides a method for detecting new antibiotic pollutants in a water body sample by combining solid-phase extraction and enzyme-linked immunosorbent assay, which comprises the following steps: (a) selecting an organic solvent, so that R2 of a standard curve obtained by enzyme-linked immunosorbent assay of a standard substance containing the new antibiotic pollutants and an aqueous solution of the organic solvent is greater than 0.98; (b) determining the optimal concentration of the organic solvent by taking the quantitative detection interval, the sensitivity, the detection limit and the precision of enzyme-linked immunosorbent assay as evaluation indexes and combining a CRITIC weight method; and (c) preparing the new antibiotic pollutants enriched by the organic solvent selected in the step (a) through solid-phase extraction of the water body sample and water into a redissolved aqueous solution, and detecting the redissolved aqueous solution through enzyme-linked immunosorbent assay to determine the concentration of the new antibiotic pollutants in the water body sample, wherein the concentration of the organic solvent in the redissolved aqueous solution is the optimal concentration. The invention also provides a method for optimizing enzyme-linked immunosorbent assay.

A pretreatment method suitable for perfluorinated and polyfluoroalkyl compounds and organophosphorus esters in a biological sample comprises the following steps: adding acetonitrile into the biological sample, adding an internal standard, carrying out vortex, centrifuging, extracting a supernatant, adding acetonitrile again, carrying out vortex, centrifuging, extracting a supernatant again, combining the two supernatants, and carrying out freeze-drying to obtain the perfluorinated and polyfluoroalkyl compounds and organophosphorus esters. And carrying out solid-phase extraction, nitrogen concentration, methanol redissolution and sample introduction analysis on the merged supernate by adopting a phospholipid small column. According to the pretreatment method, firstly, acetonitrile is used for removing protein in a biological sample, then a phospholipid removal small column is used for further purifying fat substances in a solution, it is ensured that a target analyte is not interfered, nitrogen concentration and methanol redissolution are conducted, the purity and detection sensitivity of the sample are improved, the recovery rate of PFASs and OPEs reaches the ideal range, and the method is suitable for industrial production. And reliability and effectiveness are realized.

The invention relates to the technical field of food detection, in particular to an ultra-high performance liquid chromatography-tandem mass spectrometry method for detecting hidden fungaltoxin in dried longan. According to the method, 16 kinds of hidden mycotoxins common in dried longan are taken as detection objects, a methanol-acetonitrile-water three-phase solution is adopted to efficiently extract hidden mycotoxins in a sample, food matrix interference is removed through a solid-phase extraction column, and the hidden mycotoxins are directly detected on a machine after passing through a membrane. Moreover, an ultra-high performance liquid chromatography-tandem mass spectrometry method is specially established, the detection time is only 6 minutes, and the detection efficiency is greatly improved.

The application relates to an 11-dehydrothromboxane B2 detection kit and a detection method thereof, relates to the technical field of biological detection, and comprises an activation solution, an equilibrium solution, an elution solution, an elution solution, a reconstitution solution, an internal standard solution, a calibrator and a quality control product. The detection method comprises the following steps: S1, adjusting the pH of a urine sample to 2.5-3.0 by adding phosphoric acid; S2, sucking the urine sample after pH adjustment, the calibrator and the quality control product into a centrifugal tube, reacting at 37 DEG C for 2.5-3.5 hours, adding the internal standard solution, centrifuging, and taking the supernatant for solid-phase extraction treatment; S3, solid-phase extraction; and S4, calculating the concentration of 11-DH-TXB2 in the urine according to the liquid chromatogram and the peak area of 11-DH-TXB2 in the detection sample and the corresponding internal standard peak area. The application has reliable results and is convenient to calculate.

The application discloses a controllable liquid level parallel automatic solid phase extraction instrument, and belongs to the field of analytical chemistry sample pretreatment. The extraction instrument comprises a liquid adding system, a solid phase extraction column array unit and a collecting tube device which are electrically connected with a control system. The solid phase extraction column array unit is arranged below the liquid adding system, and the collecting tube device is arranged below the solid phase extraction column array unit. The liquid adding system comprises a liquid adding pipe for adding reagents to the solid phase extraction column array unit. The extraction column has the function of automatically controlling or maintaining the liquid level, and the diameter and height of each component of the extraction column can be flexibly adjusted according to the requirement. The liquid level in the extraction column can be monitored, all the extraction columns can simultaneously and in parallel process samples, and the sample processing efficiency and reliability are improved.

This invention belongs to the field of traditional Chinese medicine processing and testing technology, and relates to an ultrasonic-assisted extraction method and content determination method for acrylamide in processed Polygonatum sibiricum. The optimal extraction process for acrylamide from processed Polygonatum sibiricum is as follows: 1.0 g of Polygonatum sibiricum, 6 mL of a mixed solution of ethyl acetate and methanol as the extractant (volume ratio 6:4), ultrasonic time of 15 min, and ultrasonic power of 180 W. After purification using a C18 solid-phase extraction column, the acrylamide content was found to be 0.498 ± 0.0015 μg / g. This method is stable and reliable. Compared with a single water extractant, using a mixed solution of ethyl acetate and methanol as the extractant significantly improves the extraction efficiency, and the operation is simple, showing good market application prospects and industrial applicability.