249 results about "Solid phase extraction" patented technology

The application provides a pretreatment and detection method of tetrabromobisphenol A and ten common derivatives thereof in sludge, which comprises the following steps: (1) sample pretreatment: after mixing the frozen and dried sludge sample and the isotopic internal standard, oscillation extraction is carried out, and the extraction liquid is obtained after centrifugation by a centrifuge; the extraction liquid is purified by solid phase extraction to obtain a sample to be detected; (2) the sample to be detected obtained in the step (1) is detected by high performance liquid chromatography-tandem mass spectrometry; (3) the content of tetrabromobisphenol A and ten common derivatives thereof in the sample is calculated according to the internal standard method. The application provides a rapid and sensitive analysis and quantitative method of tetrabromobisphenol A and ten common derivatives thereof in sludge samples.

This invention discloses a pretreatment method for detecting trace perfluorinated and polyfluoroalkyl substances (PFAS) in complex matrix textiles, belonging to the field of materials analysis technology. The method involves placing the textile in an alkaline solvent containing an alkyl sulfonate displacing agent. Under heating conditions, the displacing agent utilizes its competitive adsorption mechanism to forcibly occupy matrix adsorption sites and displace the target analytes into the solvent. Subsequently, the temperature is rapidly lowered to the phase separation induction temperature, inducing supersaturation precipitation of the displacing agent, which then encapsulates impurities in situ and co-precipitates, forming a solid-liquid stratified system. Finally, the supernatant is collected for analysis. This invention utilizes the dual properties of the displacing agent—high-temperature solubilization and low-temperature phase change removal—to effectively break the strong adsorption equilibrium of aged textiles without using a solid-phase extraction column. This solves the contradiction between low extraction rate and significant matrix interference, significantly improving the recovery rate of PFAS. It offers advantages such as low cost, simple operation, and high accuracy.

This invention relates to a system flow path for solid-phase extraction and its application method. The system flow path is characterized by a pump inlet port located below a rotary valve, several solvent inlet ports on the circumference of the rotary valve, a solvent outlet port and a waste liquid port on the circumference of the rotary valve, a gas path system connecting the solvent outlet port of the rotary valve to the solvent interface of the sample loader, and a sample loader comprising a valve core and a valve body. The top of the valve body is connected to a sample vial, and a spray needle is also located at the top of the valve body. The valve core is located within the valve body. The sample flow channel is connected to a guide tube, and a membrane adapter is located below the guide tube. The membrane adapter is connected to a collection docking point, which is connected to a dehydration device and a collection vial. The collection docking point is also connected to a flow path switching system. This invention features a compact structure, anti-clogging and anti-cross-contamination advantages, and spray cleaning of the sample vial ensures complete sample loading, improving experimental recovery rates. The online filtration and dehydration functions enable full-process automation.

The invention provides equipment and method for detecting new pollutants and a detection system. The equipment comprises a sample injection module, a multi-channel switching module, a solid-phase extraction module, a collection module, a first control module, an immunoassay sensor and a power module, and the sample injection module comprises a detection sample storage unit, an eluent storage unit and a cleaning liquid storage unit; the multi-channel switching module is at least provided with a first port, a second port, a third port and a fourth port, the fourth port is communicated with one of the first port and the second port in a switchable manner, the third port is communicated with one of the fourth port and the first port in a switchable manner, the first port is connected with the detection sample storage unit, the second port is connected with the eluent storage unit, and the eluent storage unit is connected with the detection sample storage unit. The third port is connected with the cleaning liquid storage unit; the solid-phase extraction module is provided with a feed port and a discharge port, the feed port is connected with the fourth port, and a constant volume unit is arranged at the discharge port; the first control module is connected with the detection sample storage unit, the eluent storage unit, the cleaning liquid storage unit and the multi-channel switching module.

The application belongs to the technical field of material application, and particularly relates to a graphitized carbon material and a preparation method and application thereof, the preparation method of the graphitized carbon material is as follows: (1) D-(+)-xylose powder and transition metal salt powder are weighed and uniformly ground; (2) after sieving, calcination is carried out under an inert gas atmosphere; (3) after grinding and sieving of the calcined product, washing is carried out with 1.0 mol / L dilute hydrochloric acid solution, then washing is carried out with distilled water and drying is carried out, to obtain the graphitized carbon material, the graphitized carbon material has high adsorption efficiency on aflatoxin B1 (AFB1) in vegetable oil, and in combination with magnetic solid phase extraction (MSPE) and high performance liquid chromatography-fluorescence detection (HPLC-FLD) technology, the detection limit is as low as 2.0 pg·g ‑1 The graphitized carbon material has excellent regeneration performance, and the material consumption and cost in the detection process are significantly reduced.

The application provides a method for detecting psychotropic drugs in environmental sludge based on acid pretreatment, which comprises the following steps: S1: obtaining a sludge sample to be detected, performing pressurized fluid extraction on the sludge sample to be detected after freeze-drying to obtain an extraction solution, performing solid-phase extraction on the extraction solution to obtain an eluate, and redissolving the eluate to obtain a solution to be detected, wherein the pressurized fluid extraction is performed under the condition of 5 ‰ formic acid water without heating; and S2: performing liquid chromatography mass spectrometry on the solution to be detected to obtain a mass spectrum, wherein the psychotropic drug is one of eptazocine, codeine, AMP, MAMP, MDA, monoacetylmorphine, MDMA, PMMA, fentanyl, norfentanyl, ketamine, norfentanyl, benzoyl eptazocine, cocaine and tramadol, and the method realizes detection of multiple psychotropic drugs in environmental sludge.

The invention discloses a full-automatic solid-phase extraction instrument which comprises a main machine shell and a main machine in the main machine shell, a plunger module, a test tube rack module and a concentration constant volume module in the main machine are mounted on a rack provided with various axial movement modules for continuous operation of extraction and concentration; wherein in each group of solid-phase extraction assembly in the plunger module, a solution is injected and added into the SPE column through a plunger rod; a plurality of test tubes corresponding to the solid-phase extraction assembly are arranged in the test tube rack module so as to receive corresponding filtrate; in the concentration constant volume module, corresponding elution sample liquid is received through a constant volume pipe, air blowing and liquid adding are performed by an air blowing and liquid adding assembly arranged on one side of the constant volume pipe, and then constant volume is performed on the elution sample liquid by sensing the liquid level height in the constant volume pipe through a constant volume module arranged at the bottom of the constant volume pipe. According to the invention, the structure of each module in the solid-phase extraction process is more compact, and the adaptability of the whole instrument to different samples is improved.

The invention provides a method for detecting sulfonamides in animal-derived food through capillary electrophoresis-laser-induced fluorescence, and belongs to the technical field of detection. Comprising the following steps: (1) mincing a sample, adding an extracting solution for extraction, and carrying out solid-phase extraction column extraction and methanol elution to obtain an eluted sample solution; (2) preparing a sulfanilamide drug standard substance and fluorescamine into gradient solutions by using methanol and acetonitrile respectively, and stirring at room temperature for reaction to obtain a solution A; (3) mixing the elution sample solution with a fluorescent amine-acetonitrile solution, and stirring and reacting at room temperature to obtain a solution B; and (4) detecting the fluorescence intensity of the sulfonamides in the solution A by adopting capillary electrophoresis-laser induced fluorescence to obtain a linear equation for detecting the concentration of the sulfonamides in the solution B. The invention establishes a novel method for detecting sulfonamide residues in animal-derived food by using high-efficiency capillary electrophoresis-laser-induced fluorescence, and realizes high-sensitivity detection of three sulfonamides in animal-derived food.

The invention relates to a method for detecting the content of lutein and / or zeaxanthine, which comprises the following steps: taking a standard substance of a sample to be detected, and preparing a plurality of groups of standard substance solutions with different concentrations; testing the standard substance solution by adopting an online solid-phase extraction-liquid chromatography to obtain a standard working curve; pretreating a sample to be detected to obtain a sample solution, testing the sample solution by adopting an online solid-phase extraction-liquid chromatography, and substituting the obtained result into the standard working curve to obtain the content of xanthophyll and / or zeaxanthine. According to the method provided by the invention, the separation effect between the xanthophyll and the isomer thereof is improved, the content of the xanthophyll and the isomer thereof can be accurately measured, the repeatability is good, the accuracy is high, the overall analysis time is short, and the sample analysis and detection efficiency is remarkably improved.

The invention discloses a method suitable for on-site analysis of the valence state of thallium in water. The method comprises the following operation steps: step 1, collecting and separating a water sample; step 2, acidizing treatment; 3, Tl (I) and Tl (III) are separated; 4, total thallium is measured; step 5, Tl (I) determination; the invention relates to the technical field of atomic spectrum metal element analysis, and the method has the beneficial effects that by combining an electrochemical separation technology with a Chelex-100 solid-phase extraction column and cooperating with a portable liquid cathode glow discharge thallium measurement instrument, high-sensitivity field detection of Tl (I) and Tl (III) is realized, the detection limit is lower than 1.0 g / L, and the repeatability is RSDlt; the analysis period of a single sample is less than or equal to 30 minutes, the weight of equipment is only 5kg, battery power supply is supported, and acidification-separation-detection integrated operation can be completed outdoors without professional laboratory conditions.

This invention relates to the field of high-purity material analysis technology, and discloses a method for simultaneously separating and enriching copper, cadmium, nickel, cobalt, zinc, lead, and barium in lithium carbonate. The steps are as follows: dissolving a lithium carbonate sample in nitric acid to obtain an acidified sample solution; adjusting the acidified sample solution to an alkaline pH range of 9.5-10.5 using an ammonia-ammonium acetate alkaline buffer solution; passing the alkaline solution through a solid-phase extraction column filled with iminodiacetic acid-type chelating resin, where copper, cadmium, nickel, cobalt, zinc, lead, and barium ions are selectively adsorbed onto the resin; and eluting the adsorbed heavy metal ions with acid to achieve separation and enrichment. This invention effectively overcomes the inhibition and interference of the lithium carbonate matrix on the detection of trace heavy metals, and in particular significantly improves the adsorption and recovery rate of elements such as barium. It achieves efficient separation and enrichment of multiple heavy metals in complex matrices, providing a reliable pretreatment solution for high-sensitivity and high-accuracy quality control analysis of lithium carbonate products.

The embodiment of the present application provides a positive pressure column passing device, which comprises a seat body, a hole plate and a positive pressure assembly. The positive pressure assembly has a gas inlet and a plurality of gas inlet units. The gas inlet unit comprises a gas outlet, a gas inlet cavity and a gas outlet cavity. The gas inlet cavity is communicated with the gas inlet, and the gas outlet cavity is communicated with the gas outlet. The gas outlet corresponds to the mounting hole, so as to be used for the gas to enter the solid phase extraction column. The gas inlet unit has a communication state and a separation state. The gas inlet unit is abutted with the solid phase extraction column, so that the gas inlet cavity and the gas outlet cavity are communicated, and a gas flow path passing through the gas inlet, the gas inlet cavity, the gas outlet cavity, the gas outlet and the solid phase extraction column in sequence is formed. The gas inlet unit is in the communication state. When the gas inlet unit is separated from the solid phase extraction column, the gas inlet cavity and the gas outlet cavity are separated to cut off the gas flow path, and the gas inlet unit is in the separation state. The positive pressure column passing device of the embodiment of the present application can reduce the risk of gas leakage.

The invention relates to the technical field of food detection, in particular to an ultra-high performance liquid chromatography-tandem mass spectrometry method for detecting hidden fungaltoxin in dried longan. According to the method, 16 kinds of hidden mycotoxins common in dried longan are taken as detection objects, a methanol-acetonitrile-water three-phase solution is adopted to efficiently extract hidden mycotoxins in a sample, food matrix interference is removed through a solid-phase extraction column, and the hidden mycotoxins are directly detected on a machine after passing through a membrane. Moreover, an ultra-high performance liquid chromatography-tandem mass spectrometry method is specially established, the detection time is only 6 minutes, and the detection efficiency is greatly improved.

The application relates to an 11-dehydrothromboxane B2 detection kit and a detection method thereof, relates to the technical field of biological detection, and comprises an activation solution, an equilibrium solution, an elution solution, an elution solution, a reconstitution solution, an internal standard solution, a calibrator and a quality control product. The detection method comprises the following steps: S1, adjusting the pH of a urine sample to 2.5-3.0 by adding phosphoric acid; S2, sucking the urine sample after pH adjustment, the calibrator and the quality control product into a centrifugal tube, reacting at 37 DEG C for 2.5-3.5 hours, adding the internal standard solution, centrifuging, and taking the supernatant for solid-phase extraction treatment; S3, solid-phase extraction; and S4, calculating the concentration of 11-DH-TXB2 in the urine according to the liquid chromatogram and the peak area of 11-DH-TXB2 in the detection sample and the corresponding internal standard peak area. The application has reliable results and is convenient to calculate.

The application discloses a controllable liquid level parallel automatic solid phase extraction instrument, and belongs to the field of analytical chemistry sample pretreatment. The extraction instrument comprises a liquid adding system, a solid phase extraction column array unit and a collecting tube device which are electrically connected with a control system. The solid phase extraction column array unit is arranged below the liquid adding system, and the collecting tube device is arranged below the solid phase extraction column array unit. The liquid adding system comprises a liquid adding pipe for adding reagents to the solid phase extraction column array unit. The extraction column has the function of automatically controlling or maintaining the liquid level, and the diameter and height of each component of the extraction column can be flexibly adjusted according to the requirement. The liquid level in the extraction column can be monitored, all the extraction columns can simultaneously and in parallel process samples, and the sample processing efficiency and reliability are improved.

This invention belongs to the field of traditional Chinese medicine processing and testing technology, and relates to an ultrasonic-assisted extraction method and content determination method for acrylamide in processed Polygonatum sibiricum. The optimal extraction process for acrylamide from processed Polygonatum sibiricum is as follows: 1.0 g of Polygonatum sibiricum, 6 mL of a mixed solution of ethyl acetate and methanol as the extractant (volume ratio 6:4), ultrasonic time of 15 min, and ultrasonic power of 180 W. After purification using a C18 solid-phase extraction column, the acrylamide content was found to be 0.498 ± 0.0015 μg / g. This method is stable and reliable. Compared with a single water extractant, using a mixed solution of ethyl acetate and methanol as the extractant significantly improves the extraction efficiency, and the operation is simple, showing good market application prospects and industrial applicability.

The invention relates to the technical field of heavy metal pollution treatment, in particular to a manganese slag elution method based on BCR real-time regulation and form directional transformation, which comprises the following steps: drying manganese slag, crushing, sieving and pretreating, performing form analysis on a sample by adopting a micro solid-phase extraction column-BCR method, and determining the distribution proportion of heavy metals in various forms; providing an elution reaction device; the pretreated manganese slag is introduced into an elution cavity, eluents matched with exchangeable heavy metal, reduction heavy metal, oxidation heavy metal and residual heavy metal are sequentially added into the elution cavity, real-time sampling is conducted through a monitoring cavity after precise filtration, and the concentration of target heavy metal in eluent is detected through a BCR color developing agent; when the concentration detected by the monitoring cavity is lower than a set threshold value, the eluent is switched; and filtering and separating the eluent and filter residues, and adding a curing agent into the filter residues to carry out low-temperature thermocuring treatment. The method can realize directional elution of heavy metal forms and long-acting stability of slag bodies.

The invention discloses a magnetic reduced graphene oxide / hydrophobized gold nanoparticle composite material as well as a preparation method and application thereof in chlorpromazine analysis, and belongs to the technical field of detection and analysis. The magnetic reduced graphene oxide / hydrophobized gold nanoparticle composite material comprises reduced graphene oxide, Fe3O4 particles are loaded on nanosheets of the reduced graphene oxide to form magnetic reduced graphene oxide, gold nanoparticles are modified on the nanosheets of the magnetic reduced graphene oxide, and the Fe3O4 particles are loaded on the nanosheets of the magnetic reduced graphene oxide to form the magnetic reduced graphene oxide / hydrophobized gold nanoparticle composite material. The gold nanoparticles are grafted with n-octadecanethiol through gold-sulfur bonds. The composite material disclosed by the invention has excellent magnetism, selective adsorption performance on chlorpromazine, conductivity and catalytic activity, and when the composite material is used as an adsorbent for detecting chlorpromazine in animal-derived food by a magnetic solid-phase extraction-electrochemical detection combined technology, the composite material has good anti-interference performance, accuracy and stability.

The utility model discloses a solid-phase extraction device capable of preventing splashing. The solid-phase extraction device comprises a base, a test tube with a conical opening part and a nitrogen blowing tube, through the optimized design of the chock plug structure, the holes are formed in the surface of the chock plug to allow nitrogen to be smoothly discharged after purging, and pressure abnormity caused by gas retention is avoided; meanwhile, the edge of the plug is tightly attached to the inner wall of the test tube, liquid flowing back along the tube wall is physically blocked, splashing loss and cross contamination are prevented, the dual functions of exhaust and liquid sealing are achieved, an infrared heating plate is adopted to be matched with a heat insulation layer, non-contact heating is directly conducted on the bottom of the test tube, rapid evaporation is achieved, and temperature distribution is uniform; by combining the visual design that the bottom of the test tube is completely exposed, the concentration process can be observed in real time, excessive dry burning is prevented, the background plate and the direction-adjustable LED light bar are integrated, and the liquid level observation definition is remarkably improved through the high-contrast background and lateral light supplement.

The present invention relates to a method for extracting a macrocyclic analyte from a sample, said method comprising (a) producing a lysis admixture comprising said sample, a first organic solvent at a concentration of from 2.5% (v / v) to 18% (v / v), and an aqueous component at a concentration of at least 60% (v / v), and contacting said lysis admixture with a solid-phase extraction agent; (b) obtaining solid-phase extraction agent bound sample constituents; and (c) thereby extracting said macrocyclic analyte. The present invention also relates to systems, kits, extracts, and determining methods related thereto.

The utility model discloses an auxiliary experiment tool for a solid-phase extraction device, which comprises a disassembly and assembly component, and the disassembly and assembly component comprises a filter screen, a threaded column, a connecting frame, a connecting plate and a limiting column. Through the arrangement of the dismounting assembly, when the filter screen needs to be replaced, pulling force is applied to a guide plate, a limiting rod is driven to be separated from the interior of a limiting column, then pulling force is applied to a connecting frame, the filter screen is taken out from the interior of an air inlet, rotating force is applied to the connecting frame, and a threaded column is taken out from the interior of the filter screen; according to the scheme, a threaded column is rotated into a new filter screen, pulling force is applied to a guide plate, then the filter screen is placed into an air inlet, after a limiting column penetrates through a connecting plate and a connecting frame to be attached to the surface of the connecting plate, the force applied to the guide plate is canceled, and under the elastic force effect of a spring, a limiting rod rebounds into the limiting column. The filter screen can be quickly and simply disassembled and assembled, and the efficiency of replacing the filter screen is improved.

The invention provides a method for simultaneously detecting dichloroacetic acid and trichloroacetic acid in urine, and belongs to the technical field of analysis detection and occupational health / environmental health. According to the method disclosed by the invention, a synergistic pretreatment strategy of acidic sample loading, WAX / reversed-phase mixed mode solid-phase extraction, water / low-proportion organic phase step-by-step leaching and ammonia-water-containing organic phase elution is adopted; a technical route of protein precipitation, acidification pH regulation, purification and enrichment by adopting WAX / reversed-phase mixed mode solid-phase extraction on a 96-well plate and stable isotope internal standard isotope dilution LC-MS / MS quantification is adopted, the method can effectively reduce the matrix effect and realize high-throughput analysis in a high-salinity urine matrix, the quantitation limits of DCEA and TCAA are not higher than 0.30 g / L and 0.20 g / L respectively, and the method has the advantages that the method is simple and convenient to operate, and the method is suitable for large-scale popularization and application. The method is suitable for biological monitoring and risk assessment of occupational and environmental low-level exposure crowds.

The invention discloses a post-AMI heart failure oxidized lipid screening method based on solid phase extraction, LC-MS / MS and AI. The method comprises the steps of blood sample pretreatment, solid phase extraction and purification, LC-MS / MS detection and AI model screening. According to the method, high-sensitivity qualitative and quantitative detection of oxidized lipid is realized through optimized LC-MS / MS parameters, and key oxidized lipid molecules and risk levels of heart failure after AMI emergency treatment blood supply reconstruction can be quickly and accurately screened in combination with a trained random forest AI model; the kit solves the problems that in the prior art, trace oxidized lipid detection sensitivity is low, specificity is poor, and heart failure risk screening lags, has the advantages of being high in detection efficiency, good in accuracy and high in practicability, can provide a scientific basis for early warning and intervention of clinical heart failure after AMI, and has high clinical application value.

The invention relates to the technical field of food detection, discloses an efficient detection method for six sugar alcohol compounds in dairy products, and aims to solve the problems that in the prior art, dairy product protein precipitation and high-efficiency dissolution of strong-polarity sugar alcohol are difficult to consider at the same time, and toxic precipitants, high-temperature treatment or complex solid-phase extraction are often depended on. The problems of tedious operation, high equipment threshold and poor detection stability exist in the prior art, trichloroacetic acid with optimized concentration is used for constructing a water phase system, and dissolution of six sugar alcohols and preliminary precipitation of protein are synchronously realized; the high-protein matrix of the milk powder is supplemented with precise supplementary precipitation, and the liquid milk is simplified; a specific chromatographic column and gradient elution are combined, linear correlation is established by adopting an external standard method, synchronous detection of six sugar alcohols is completed, high-temperature and solid-phase extraction consumables are not needed, two matrixes of milk powder and liquid milk are adapted, the operation time is short, the detection recovery rate is high, the precision is excellent, the existing pain point is effectively solved, and the batch detection requirement of a conventional laboratory is met.

The application discloses a method for detecting a plurality of alkyl imidazole ionic liquids in serum based on liquid chromatography-mass spectrometry, and comprises the following steps: preparing a matrix standard solution, and drawing a matrix standard curve; carrying out pretreatment and solid phase extraction on a human serum sample to be detected; and finally, performing quantitative analysis by using LC-MS / MS. The method can quickly and accurately detect the alkyl imidazole ionic liquids in the human serum sample, is simple, efficient and high in sensitivity, can realize the detection of 10 target alkyl imidazole ionic liquids in human exposure, is good in separation effect, can provide an effective technical means for the biological monitoring research work of ionic liquid pollutants, and in the theoretical aspect, provides a certain scientific basis for the human exposure risk and preventive measures of ionic liquids.

The invention relates to a method for detecting various free androgens in serum, which comprises the following steps: carrying out ultrafiltration treatment on a sample to obtain an ultrafiltration solution; magnetic beads are added into the ultrafiltration solution, and androgen is extracted through magnetic solid-phase extraction; adding a derivatization reagent containing quaternary ammonium oxygen amine into the extracted androgen, and carrying out oximation reaction; and injecting the solution after oximation reaction into a high performance liquid chromatography-tandem mass spectrometry system for detection. The method provided by the invention has excellent specificity and sensitivity, the linear fitting correlation coefficient (r) is greater than 0.99, the recovery rate of the extract is higher than 95.1%, the precision and accuracy measured by relative errors and variable coefficients both meet the regulations of the clinical and laboratory standard association (CLSI), and a stability test proves that under typical clinical treatment conditions, the analyte loss is extremely small.

The utility model discloses a two-layer automatic solid-phase extraction instrument which sequentially comprises a first extraction column platform, a second extraction column platform and a collection platform from top to bottom, wherein the first extraction column platform is used for mounting a first extraction column array; the second extraction column platform is used for mounting a second extraction column array and a first collection pipe array; a first liquid adding platform and a second liquid adding platform are respectively arranged above the first extraction column platform and the second extraction column platform, liquid adding pipes are arranged on the first liquid adding platform and the second liquid adding platform, and a front-back moving mechanism for driving the second extraction column array and the first collecting pipe array to move front and back is arranged on the second extraction column platform; according to the scheme, double-column continuous solid-phase extraction of a complex sample can be realized through the first extraction column platform and the second extraction column platform; meanwhile, the scheme can realize rapid collection of primary elution curve component liquid and secondary elution curve component liquid of double-column continuous solid-phase extraction, helps researchers to understand the separation condition of each component in a sample, and qualitatively and quantitatively analyzes a target object.

The application discloses a detection method for separating diglyceride in vegetable oil and qualitative analysis of the diglyceride, and the diglyceride in the vegetable oil is dissolved by using n-hexane to obtain a sample for standby; different proportion eluent is used to wash and elute a solid phase extraction column, and eluent is collected and dried for standby to obtain eluate; the eluate is analyzed and quantified by using a thin layer chromatography and gas chromatography, and finally, the eluate is finely qualitatively analyzed by using a triple quadrupole; the separation method is simple, the separation of the diglyceride in the vegetable oil can be realized by using two extraction cartridges, the test steps are simplified, the separation purity is high, the cost and time are reduced; finally, qualitative analysis is performed by using GC-MS, the analysis time is short, the specific fatty acid composition in the diglyceride is more finely analyzed, and the qualitative analysis detection of food nutrition and the guidance of related food production are facilitated.

The present application relates to water body eDNA filter, online collection and solid phase extraction enrichment integrated device, method and application, and belongs to the technical field of environmental equipment. The filter comprises detachably and sealingly connected filter upper shell and lower shell, the filter upper shell or / and the lower shell is provided with a filter plate, and the filter upper shell and the lower shell are respectively provided with a water inlet and a water outlet; the filter lower shell is further provided with a counterweight. The integrated device comprises the water body eDNA filter and is connected with the water collector through the communication pipe, the water collector is connected with the negative pressure pump and the solid phase extraction instrument through the communication pipe, and the negative pressure pump is further connected with the power supply. The water body eDNA filter can realize in-situ filtering and collection of water body eDNA. The water body eDNA online collection and solid phase extraction enrichment integrated device realizes the synchronous completion of water body eDNA capture and dissolved matter enrichment in the field through the integrated fluid path design.

The present application relates to the technical field of nitrofurans metabolite content determination, and particularly relates to a method for determining the residual amount of nitrofurans metabolite in propolis. The method is that the pretreated propolis sample is detected by liquid chromatography-tandem mass spectrometry after gradient elution of the mobile phase; the pretreatment is that the propolis sample is sequentially hydrolyzed by hydrochloric acid, purified by HLB solid phase extraction column, derivatized by o-nitrobenzaldehyde, and purified by ethyl acetate treatment. The present application purifies the propolis sample after hydrochloric acid hydrolysis before o-nitrobenzaldehyde derivatization, so that o-nitrobenzaldehyde fully reacts with the nitrofurans metabolite in propolis, and the residual amount of nitrofurans metabolite in propolis is accurately detected by combining the selection and gradient elution of the mobile phase.