64 results about "Electrospray" patented technology

The present application relates to the technical field of microsphere preparation, and particularly relates to a microsphere-based electrospray device, which comprises a nozzle, a conductive piece and a power supply, the nozzle has a solution channel, the solution channel has an inlet and an outlet, the conductive piece is arranged at a position adjacent to the outlet, the power supply comprises a first output end and a second output end, the first output end is connected with the outlet to provide a first electric field, the second output end is connected with the conductive piece to provide a second electric field, and the direction of the first electric field is arranged orthogonally to the direction of the second electric field, the microsphere-based electrospray device can improve the uniformity of the prepared microspheres.

The application provides a kind of ionic liquid electric spray micro force calculation method, device and electronic equipment, it is related to data processing field, the method is carried out original molecular dynamics simulation in the negative gradient calculation of position to atomic potential energy and obtains the force model, the micro force decoupling of ionic liquid electric spray under molecular dynamics simulation is executed;Based on the micro force model, the molecular dynamics trajectory data is recalculated by modifying the molecular dynamics simulation code parameters, the pre-data of different micro forces is obtained, and the atomic force calculation result is output based on the pre-data of different micro forces;The atomic force calculation result corresponding to the steady state part of system is calculated by time and space average, and the spatial distribution calculation result of micro force and electric field is obtained;After a spatially uniform distribution of constant electric field is applied to the system, the first external electric field received by the charged atom is calculated using the above scheme, and the applied electric field and the calculated electric field are consistent, which verifies that the scheme is executed without error.

The application provides a detection method of hydroxypropyl cellulose in a povidone-containing preparation, and specifically comprises the following steps: (1) preparing a sample solution by pretreating a sample to be detected; (2) detecting the sample solution by using HPLC, wherein an evaporative light scattering detector or an electrospray detector is used as a detector, a C18 chromatographic column is used as a chromatographic column, the column temperature is 20-40 DEG C, water is used as a mobile phase A, methanol is used as a mobile phase B, the flow rate is 0.5 ml / min-1.5 ml / min, and gradient elution is adopted. The detection method has good specificity and peak type, can not only separate hydroxypropyl cellulose from povidone and other high-molecular auxiliary materials in a pharmaceutical preparation, accurately quantify the content of hydroxypropyl cellulose, but also effectively identify the model, and provides a good method for reverse analysis, component analysis, auxiliary material release detection of the pharmaceutical preparation.

The invention discloses a plasma electrospray fusion ionization source and mass spectrum combined system and a detection method. The plasma electrospray fusion ionization source and mass spectrum combined system comprises a coaxially nested electrospray needle, a sample injector, an ultrahigh voltage generator, a grounded metal grid mesh, a mass spectrometer and a detector thereof. A grounded metal grid is installed between a spray needle and a mass spectrum inlet, voltage is applied to the spray needle, carrier gas is excited to generate plasmas, the macroscopic plasmas at the front end of the spray needle are fused with electrospray, and the plasmas and micro-droplets at the tip of the spray needle are fused. According to the unique ionization source constructed by the plasma-micro-droplet fusion system, on one hand, the ionization efficiency is improved, mass spectrum signals are enhanced, the application range of analytes is widened, excellent sensitivity to high-polarity compounds is kept, and effective ionization and detection of low-polarity compounds are achieved; on the other hand, higher energy and active species are provided for the micro-droplet reaction, the energy barrier required by traditional synthesis is broken, and efficient spontaneous product generation of the micro-droplet chemical reaction is achieved.

The invention relates to the technical field of tobacco chemical analysis, in particular to a multistage mass spectrometry identification method for chemical structures of sucrose esters in tobacco and tobacco products, which comprises the following steps: crushing and extracting a tobacco product sample; carrying out solid-liquid separation and filtration to obtain a liquid to be detected; separating the liquid to be detected by adopting hydrophilic interaction chromatography; performing triple quadrupole-ion trap tandem mass spectrometry analysis on the components separated in the step S2 by adopting an electrospray ion source in a positive ion mode to sequentially obtain mass spectrometry information of parent ions, daughter ions and grandchild ions; and determining the chemical structure of the sucrose ester based on the mass spectrum information of the parent ion, the daughter ion and the grandchild ion measured in the step S3. According to the method, complex pretreatment steps are not needed, sucrose esters with different configurations can be identified more efficiently, interference can be effectively eliminated, and substituent group structure information of a low-content target object can be scientifically confirmed.

A method is disclosed comprising providing a biological sample on a swab, directing a spray of charged droplets onto a surface of the swab in order to generate a plurality of analyte ions, and analysing the analyte ions.

The application belongs to the field of advanced manufacturing technology, and proposes a micro-nano electro-fluid jet printing method based on electric field focusing and deflection control. The method uses double circular rings as induction electrodes to induce the printing of charged droplets with micro-nano scale resolution at the metal micro-tip of the ink, and then uses the time-varying pulse voltage signal generated by the jet deflection electrode to control the flight trajectory of the droplets, so as to print high-precision micron patterns on the stationary receiving plate. Combined with the movement of the work platform, macro-scale structures can be further manufactured. The method breaks through the limitation that the trajectory movement of the work platform is necessary for the electro-fluid jet printing of micron patterned structures, and can realize the deposition of any pattern on a stationary platform, and has the characteristics of high printing resolution, high precision and high efficiency.

The invention discloses a lithium manganate positive electrode material, a preparation method and an applied lead-lithium battery, relates to the technical field of lithium manganate positive electrode materials, and solves the problems that the existing lithium manganate positive electrode material is poor in structure and interface stability and poor in rate and cycle performance. The positive electrode material is composed of a lithium manganate matrix and a double-layer composite coating, the matrix is prepared by taking lithium carbonate and manganous-manganic oxide as raw materials and doping magnesium hydroxide, aluminum hydroxide and titanium dioxide multi-plasma phase; the lithium manganate positive electrode material is prepared by uniformly compounding a lithium manganate matrix modified by a spinel inner layer, vinyl functionalized MOF and a polyvinylidene fluoride-hexafluoropropylene copolymer on the surface of an aluminum foil receiver through an electrospinning-electrospray synergistic process. The material is applied to the lead-lithium battery, shows excellent electrochemical performance and has good practical application value.

The application discloses a low-cost electrospray device for capturing nanoparticles in a vacuum system, which comprises a micro-injection pump, a stainless steel capillary, a high-voltage direct-current power supply and a vacuum cavity. The micro-injection pump is used to slowly extrude a syringe to provide a stable flow rate of a nanoparticle solution for the device. A conductive wire is connected to the liquid through a PEEK tee connector to charge the liquid. The vacuum cavity is located behind the electrospray and has a pressure lower than the atmospheric pressure. The charged liquid is attracted by a grounded metal ring after flowing out of the stainless steel capillary, breaks through the surface tension and forms a uniform spray to pass through the middle of the grounded metal ring. The electrospray is sucked into the vacuum cavity due to the pressure difference. A quadrupole rod is arranged in the vacuum cavity to guide and store the nanoparticles. The device can form a stable and continuous nanoparticle electrospray by providing a lower voltage, so as to realize the loading of the nanoparticles in the vacuum.

The invention provides an ionic liquid electrospray microcosmic stress calculation method and device and electronic equipment, and relates to the field of data processing.The method comprises the steps that negative gradient calculation of the position of atomic potential energy is conducted to obtain an acting force model in original molecular dynamics simulation, and microcosmic acting force decoupling of ionic liquid electrospray under molecular dynamics simulation is executed; re-calculating the molecular dynamics trajectory data by modifying molecular dynamics simulation code parameters based on the microscopic acting force model to obtain preposed data of different microscopic acting forces, and outputting atomic acting force calculation results based on the preposed data of the different microscopic acting forces; carrying out time and space average calculation on the atomic acting force calculation result corresponding to the steady-state part of the system to obtain a microscopic acting force and an electric field space distribution calculation result; after a constant electric field which is uniformly distributed in space is applied to the system, the first external electric field borne by the charged atoms is calculated by using the scheme, and if the applied electric field is consistent with the calculated electric field, the scheme execution is correct.

The application discloses an electric spray thruster, relates to the technical field of electric thrusters, and can quickly position an emitter and an extraction grid, saves the mounting time of the thruster, and improves the consistency of the positions of the emitter and the extraction grid. The electric spray thruster comprises a shell, an emitter, a high-voltage electrode and an extraction grid, the shell has a containing cavity for containing propellant, and the inner wall of the shell is provided with a first limiting surface. The emitter is arranged in the containing cavity, and one end of the emitter is located below the liquid level of the propellant. The high-voltage electrode is fixedly sleeved outside the emitter, and the high-voltage electrode is arranged on the inner wall of the shell. The outer wall of the high-voltage electrode is provided with a second limiting surface which is limitedly matched with the first limiting surface and is used for limiting the relative position of the high-voltage electrode and the shell. The extraction grid is arranged at the outlet end of the shell, and one side of the extraction grid close to the containing cavity is provided with a protruding portion which extends into the containing cavity, the protruding portion is provided with a third limiting surface which is limitedly matched with the first limiting surface and is used for limiting the relative position of the extraction grid and the shell.

This invention proposes a polybenzimidazole microporous ion-conducting membrane and its one-step electrospray preparation method. Polybenzimidazole electrospray solutions are prepared using organic solvents with different boiling points. Electrospray parameters are adjusted under a high-voltage electrostatic field, and the polybenzimidazole electrospray solution is dispersed into nanodroplets using this field. A receiving roller rotates to spread and layer the droplets, followed by rapid drying and solidification, directly preparing the polybenzimidazole microporous membrane. This invention can directly prepare microporous membranes using non-ionic polymer materials, and the membrane preparation process is simple and easily scaled up. It overcomes the problem of high mass transfer resistance of non-ionic polymers, enabling highly selective proton conduction. Simultaneously, the high stability of non-ionic polymers improves the oxidative stability and mechanical strength of the ion-conducting membrane, showing broad application prospects in the field of new energy storage flow batteries.

The application provides a lung cancer intelligent screening model construction method and system based on sputum direct mass spectrum data. The method comprises the following steps: S1, obtaining the original mass spectrum data of the sputum sample detected by neutral desorption electrospray extraction ionization mass spectrometry, preprocessing including mass spectrum peak detection, peak alignment and intensity normalization to form an initial feature matrix; S2, extracting a core metabolic fingerprint feature set from the initial feature matrix; S3, constructing and training a heterogeneous integrated diagnosis model; S4, optimizing and verifying the hyperparameters of the heterogeneous integrated diagnosis model, and packaging the optimized heterogeneous integrated diagnosis model and the screening rules of the core metabolic fingerprint feature set into a lung cancer screening deployment module. The application realizes high-robustness, high-precision and high-explainability lung cancer non-invasive intelligent screening.

The invention belongs to the technical field of mass spectrum imaging, and discloses a desorption electrospray-dielectric barrier discharge combined ionization source mass spectrum imaging device, which comprises a desorption electrospray mechanism and a dielectric barrier discharge mechanism, and is characterized in that the desorption electrospray mechanism comprises an insulating tube, a desorption capillary tube, a direct-current high-voltage power supply, a gas conveying tube and a sample carrying table; the dielectric barrier discharge mechanism comprises a capillary tube, an inner electrode, an outer electrode and a radio-frequency alternating-current high-voltage power supply, one end of the capillary tube is arranged towards the sample carrying platform, and in the mass spectrum imaging process, the device can perform secondary ionization on charged liquid drops sputtered by desorption electrospray, so that the ionization efficiency is remarkably improved; and high-efficiency ionization of weak polar and non-polar molecules in a sample can be realized, so that synchronous analysis of polar metabolites and non-polar metabolites is supported.

The invention relates to the technical field of ion source spray needles, in particular to a quick release type ion source spray needle which comprises an ion source support, an electrospray ion source fixedly installed at the bottom, a quick installer fixedly installed on the outer side of the bottom and an installation connector fixedly installed at the bottom, and a spray needle assembly is inserted into the installation connector. The spray needle assembly is mounted at the bottom of an electrospray ion source through a quick mounting device. When the spray needle body needs to be dismantled, the hand-operated grab bar can be pushed by manually pulling the hand-operated grab ball to drive the quick-release linkage frame body to move downwards, the displacement block slides in the guide rail for guiding to enable the quick-release linkage frame body to move downwards stably, and the bottom inclined face of the quick-release linkage frame body makes contact with the inner end arc face of the guide frame body for guiding; the guide frame body is opened to enable the two sides of the guide frame body to slide, the sliding block is pushed to extrude the limiting spring, the clamping block at the end of the limiting frame is separated from the clamping groove, the spray needle connector loses limitation, and the spray needle assembly is rapidly disassembled.

The invention belongs to the technical field of tobacco chemistry, and particularly relates to a method for detecting the content of spermidine compounds in tobacco leaves. The invention provides a method for determining the content of spermidine compounds in tobacco leaves, which adopts ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry to quantitatively detect the content of spermidine compounds in a sample solution to be detected by an external standard one-point method, the flowing phase B is selected from one or more of formic acid, acetic acid and a mixture of formic acid and water, the sample size is 0.5-3 [mu] L, the flow velocity is 0.2-1.0 mL / min, the elution mode is gradient elution, the ion source is an electrospray ion source, the scanning mode is positive ion scanning, and the detection mode is multi-reaction monitoring. According to the method, extraction conditions are optimized, the detection method has high sensitivity and accuracy, the content of multiple spermidine compounds in the tobacco leaf sample can be quantitatively detected at the same time, and the method is suitable for detection of large-batch tobacco leaf samples.

The invention provides a micro-channel-porous emission structure for an array type ionic liquid electronic injection thruster. The micro-channel-porous emission structure comprises a substrate, an emission component, a flow guide component and a storage component. The adjacent emission components are communicated through the flow guide component, and the storage component is arranged on the flow guide component, so that cooperative work of the flow guide component and the storage component is realized, and when the ionic liquid is not completely erupted or is sprayed back and is gathered near the emission components and on the substrate, the flow guide component can guide the ionic liquid into the storage component, so that the ionic liquid can be stored in the storage component. And the data is stored in the storage component. Compared with a traditional array type electrospray thruster which does not adopt a special structure for preventing liquid overflowing on the surface of an array type substrate, the working mode that the flow guide component and the storage component cooperate is adopted, so that the collection and storage capacity of the overflowing liquid is improved; and it is guaranteed that no redundant liquid is gathered at the bottom of the emitter during large-flow or long-time work, and it is guaranteed that the thruster stably generates thrust.

The application relates to a method for quantitatively detecting the concentration of thioctic acid in human plasma by liquid chromatography-tandem mass spectrometry, and belongs to the technical field of biological sample detection. The method comprises the following steps: EDTA-K2 anticoagulant human plasma is taken, thioctic acid-d5 internal standard working solution is added, the mixture is uniformly mixed, acetonitrile is added to precipitate proteins, the supernatant is taken by centrifugation and is diluted; a C18 chromatographic column is used, 0.1% acetic acid aqueous solution and acetonitrile are used as mobile phases for gradient elution; mass spectrometric detection adopts an electrospray ion source negative ion mode and a multiple reaction monitoring mode, the ion pair of thioctic acid is m / z 205.100->171.100, and the ion pair of the internal standard is m / z 210.050->174.100; the peak area ratio is used as a quantitative index, 1 / C 2 weighted linear regression is used to establish a standard curve. The method has the advantages of simple pretreatment, high sensitivity, rapid analysis, strong anti-matrix interference ability, and is suitable for the detection of conventional and hemolytic and hyperlipidemic plasma samples, and can be used for pharmacokinetic research and clinical detection of thioctic acid.

The present application relates to the technical field of health product ingredient detection, in particular to a detection method of a new type of sildenafil compound in health food, comprising the following steps: qualitative and quantitative detection by HPLC-MS / MS; the chromatographic conditions include: a chromatographic column, a C18 column; a column temperature, 40 DEG C; a mobile phase A, 0.1% formic acid aqueous solution; a mobile phase B, acetonitrile; a flow rate, 0.5 mL / min; a gradient elution program, 0~2.0 min, 10%B~95%B; 2.0~5.0 min, 95%B; 5.0~5.1 min, 95%B~5%B; 5.1~8.0 min, 5%B; a sample injection amount, 5 µL; the mass spectrometry conditions include: an ionization mode, an electrospray ionization source, a positive ion mode; an electrospray voltage, 5500 V; an ion source temperature, 550 DEG C; an atomization gas pressure, 345 kPa; an auxiliary gas pressure, 414 kPa; a curtain gas pressure, 276 kPa; a declustering voltage, 100 V; a collision energy, 45 V; a quantitative ion pair, 449 / 243; a qualitative ion pair, 449 / 259; the residence time of each ion pair, 250 ms; the new type of sildenafil compound contains a methyl piperazine group. The detection method of the present application is simple in operation, short in time consumption, high in sensitivity and good in stability.

The application discloses a kind of HPLC-MSMS method for simultaneously determining valproic acid, olanzapine and its metabolites in plasma, which comprises the following steps: S1. pretreatment of plasma samples with acetonitrile protein precipitation method;S2. HPLC-MSMS method is used to detect plasma samples, and qualitative and / or quantitative analysis of valproic acid, olanzapine and its metabolites in plasma is carried out simultaneously;Wherein, Agilent Poroshell 120EC-C18 column is selected as the chromatographic column;Electrospray ion source is used, and the mobile phase is A phase: formic acid water, B phase: acetonitrile;By optimizing the conditions of liquid phase and mass spectrometry, valproic acid, olanzapine and its metabolites are simultaneously determined by one method.The method has been applied to detect plasma samples of olanzapine and valproic acid combination, and olanzapine alone, and the measured results all meet the linear range.

A lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe and a detection method of sugar components contained therein, comprising: water decoction extraction and concentration of traditional Chinese medicine decoction pieces constituting the lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe to obtain water extract dry products, preparation of standard solution from the water extract dry products, liquid chromatography-electrospray detection by high performance liquid chromatography-electrospray detector to obtain standard liquid chromatogram and mark the characteristic peaks therein, then comparison with the liquid chromatogram of the detected decoction / patent medicine obtained by the same method to determine whether the detected decoction / patent medicine belongs to the lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe, and to determine the six sugar substances contained in the detected decoction / patent medicine, and further comparison with the area of the corresponding sugar characteristic peak in the standard liquid chromatogram to determine the content of the corresponding sugar. The detection method and the standard provided by the application can be used as the basis for formulating the quality control standard and the quality detection standard of the lipid-lowering traditional Chinese medicine Qingzhi Huayu recipe decoction / patent medicine.

The invention discloses a method for simultaneously determining seven mass markers (Q-Marker) in serum of traditional Chinese medicine particles for treating cervical pain, which is characterized in that the seven mass markers are magnoflorine, puerarin, tetrahydropalmatine, paeoniflorin, notoginsenoside R1, ligustilide and decuricin; the method comprises the following steps: adding a serum sample into acetonitrile and acetone, carrying out vortex and centrifugation, taking a supernatant, loading the supernatant into a Hybrid SPEcolumn, washing with water to remove salt and polar interferents, eluting a target analyte with acetonitrile, filtering the eluent through a microfiltration membrane, carrying out sample introduction determination, and carrying out quantitative analysis by adopting a high performance liquid chromatography-mass spectrometry method and an electrospray ion source. And quantitative analysis is carried out in a positive / negative ion multi-reaction monitoring detection mode. The method is efficient, sensitive and high in selectivity, and solves the problems of serious matrix interference, indefinite target components, difficulty in simultaneous analysis of multiple components and the like in the prior art.

The invention belongs to the technical field of metallurgy, and discloses a method for comprehensively recycling acid-making electrospray slag. The method comprises the following steps: S1, slurrying: mixing and slurrying the electric mist slag, mercury-containing crude selenium and concentrated sulfuric acid to obtain a slurried material; s2, primary acidification roasting: feeding the pulpified material into a roasting furnace for roasting at the roasting temperature of 220-320 DEG C for 20-60 hours, and in the roasting process, the negative pressure in the furnace is 0-50 Pa; primary roasting slag is obtained; settling roasting flue gas to recover selenium dioxide; s3, secondary roasting is conducted, specifically, primary roasting slag is roasted again, the roasting temperature is 340-440 DEG C, the roasting time is 3-10 h, and the negative pressure in the furnace is-50--200 Pa in the roasting process; secondary roasting slag is obtained; and recovering mercury-containing crude selenium from roasting flue gas. By means of the method, effective separation and efficient recovery of selenium and mercury are achieved, and valuable metals such as selenium, mercury, lead, gold and silver are recovered in an echelon mode.

An electrospray device (130) includes a capillary tube (102) having an outlet orifice (108) for electrospraying a sample therefrom, and a conduit (112) extending into the capillary tube (102) in a manner that enables delivery of the sample into the capillary tube (102). The apparatus (130) may be configured to supply pressurized gas into the capillary tube (102) so as to force the sample within the capillary tube (102) toward the outlet orifice (108). The apparatus may include a sheath member (203) surrounding at least a portion of the capillary tube (102), where the sheath member (102) has one or more apertures (213) therein to permit observation of the capillary tube (102), and may include a connector element (205) for connecting the sheath member (203) to position the capillary tube within the electrospray apparatus (130).

In one embodiment, systems and methods include an electrospray device for atomizing fluids comprising a body defining a resonant microwave cavity, wherein the body comprises a radio frequency inlet configured to inject a radio frequency signal received from a power source into the resonant microwave cavity, wherein the resonant microwave cavity is configured to to amplify a voltage of the radio frequency signal. The device further comprises a field concentrating pole disposed within the resonant microwave cavity configured to concentrate the amplified voltage at a first end of the field concentrating pole to form an electric field. The device further comprises a nozzle disposed at the first end of the field concentrating pole and a fluid supply line configured to provide a fluid disposed within the field concentrating pole and fluidly connected to the nozzle, wherein the fluid supply line is fluidly connected to a fluid source.

The utility model discloses an efficient electrospray detector, and relates to the technical field of electrospray detectors. The detector comprises a detector main body, a display fixedly arranged at the top of the front end of the detector main body, and a storage box fixedly arranged at the back of the detector main body, an air cylinder is fixedly arranged on one side of the bottom of the material storage box, a lifting plate is fixedly connected to the bottom of the air cylinder, a material conveying barrel is connected to the bottom of the lifting plate through a pipeline, electric spraying heads are distributed below the material conveying barrel, a contact head is fixedly arranged in the middle of the bottom end of the material conveying barrel, and a material conveying channel is formed in the inner side of the contact head; and one end of the conveying channel is communicated with a groove. Through the arrangement of the contact head, the extrusion head, the sealing plug, the electromagnetic valve and other structures, the situation that materials overflow can be effectively prevented when the materials are added into the electric spray head subsequently can be facilitated, that is, the channel can be closed after the interior of the electric spray head is filled; and material overflow caused by excessive subsequently added materials is prevented.

Described herein is an alternating current electrospray technology comprising a hydrophobic nanoporous membrane that can generate monodisperse micron sized droplets at remarkably high throughput. The droplets can be used for ddPCR applications or for encapsulating extracellular vesicles or cells.

The invention relates to a method for detecting pesticide residues of agricultural products, and solves the bottleneck of poor stability of cross-agricultural-product detection in a rapid agricultural-product pesticide detection process. The method comprises the following steps: constructing a mass spectrum dictionary in a training mass spectrum set range; using the training mass spectrum set to train a mass spectrum recognition model under the guidance of the mass spectrum dictionary to perform pesticide category recognition according to the mass spectrum; pesticide residue detection by applying the mass spectrum recognition model comprises the following steps: scraping a sample from a set sampling position on the surface of a to-be-detected agricultural product by using a sampling probe; the sampling probe after sampling is directly inserted into a thermal desorption electrospray ion source for thermal desorption electrospray ionization, and ionized set charged ions enter a triple quadrupole mass spectrometer for mass spectrometric detection to obtain mass spectrometric data to be detected; and providing to-be-detected mass spectrum data to the mass spectrum identification model for pesticide identification. And designing a mass spectrum dictionary to guide a mass spectrum recognition model to learn pesticide characteristics in a training stage so as to improve the effect of recognizing pesticides according to the rapid pesticide residue detection mass spectrum.

This invention belongs to the field of food processing technology, specifically relating to a low molecular weight *Hydrangea hygroscopica* polysaccharide microsphere for relieving fatigue, its preparation method, and its application. The preparation method includes the following steps: (1) extraction of crude polysaccharide from *Hydrangea hygroscopica*; (2) degradation; (3) encapsulation: the low molecular weight *Hydrangea hygroscopica* polysaccharide obtained in step (2) is dripped into CaCl2 solution through electrospray treatment to form a gel core, and the gel core is encapsulated to obtain the low molecular weight *Hydrangea hygroscopica* polysaccharide microspheres. The beneficial effects of this invention are: this invention efficiently extracts *Hydrangea hygroscopica* polysaccharide through steam explosion-pulsed electric field synergy, accurately prepares 10-20 kDa high-activity low molecular weight fragments through compound enzymatic hydrolysis-ultrasound-ultrafiltration, and finally constructs colon-targeting microspheres using electrospray-layer-by-layer self-assembly. These microspheres can protect the active ingredients and allow them to reach the colon for release, significantly improving the bioavailability and anti-fatigue effect of the polysaccharide.

The invention relates to a method for determining the lipid content in lipid nanoparticles based on RP-UPLC and CAD combination and application. The method comprises the following steps: detecting a lipid nanoparticle test solution and a standard solution by adopting a reversed-phase high performance liquid chromatography and electric fog detector combination method; the detection conditions of the reversed-phase high performance liquid chromatography are as follows: a phenyl column is adopted as a chromatographic column, triethylamine acetate-water is adopted as a mobile phase A, and a triethylamine acetate-acetonitrile solution is adopted as a mobile phase B for gradient elution. The analysis method is good in repeatability and wide in application range, not only can be used for measuring the lipid content in mRNA-LNP, but also is suitable for quantification of lipid in unwrapped nucleic acid drugs, and has important significance for guiding process research and development and formula identification of lipid nanoparticles in the production process.