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3 results about "Matrix-assisted laser desorption/ionization" patented technology
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In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft (low fragmentation) ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions.
A nano-carbon powder-ionic surfactant mixed matrix, and a preparation method and application thereof
The present application relates to a kind of nanometer carbon powder-ion type surfactant mixed matrix and its preparation method and application.The mixed matrix includes nanometer carbon powder and surfactant with the molar ratio of (400-600):1.The preparation method of the mixed matrix includes the following steps: according to molar ratio, nanometer carbon powder is dispersed with surfactant in pure water, ultrasonic oscillation, and mixed solution is obtained;After the mixed solution is stirred, centrifugal, and nanometer carbon powder-ion type surfactant mixed matrix solution is obtained.The mixed matrix is applied in matrix assisted laser desorption ionization mass spectrometry detection, specifically including the following steps: mixed matrix coating: take mixed matrix in spray gun, spray on target plate, and dry after spraying;Sample coating: the sample to be measured is coated on the target plate covered with mixed matrix coating, and positive ion mode mass spectrometry analysis is carried out after drying.Compared with prior art, the present application has the advantages of simple preparation, low cost, good stability, can effectively improve peak intensity and signal-to-noise ratio, etc.
Owner:SHANGHAI INST OF TECH
Hepatitis B virus infection rapid diagnosis system combining machine learning and peripheral serum MALDI-TOF MS
PendingCN121617482AEnsemble learningMaterial analysis by electric/magnetic meansProtein profilingHepatitis B immunization
The invention discloses a hepatitis B virus infection rapid diagnosis system combining machine learning and peripheral serum MALDI-TOF MS (Matrix Assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry). On the basis of an HBV peripheral blood serum MALDI-TOF MS proteomics database, a LightGBM algorithm is utilized to construct an HBV infection machine learning prediction model, the AUC of the model is 0.88, and the F1 Score is 0.79. The HBVpred performs addition value standardization on multiple pieces of protein fingerprint spectrum data of a single peripheral blood serum sample, calls the models respectively, performs intelligent statistics and outputs a prediction result. Compared with the prior art, the HBVpred system has the advantages of high flux, short time consumption, low cost, high specificity, high identification accuracy, simplicity in operation and the like, and has a huge application prospect.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Method for improving accuracy of MALDI-TOF MS (Matrix-Assisted Laser Desorption /
PendingCN121678814APreparing sample for investigationMaterial analysis by electric/magnetic meansRapid identificationPhysical chemistry
The invention relates to a method for improving the accuracy of MALDI-TOF MS (Matrix Assisted Laser Desorption / Ionization According to the method, capsular polysaccharide of bacteria is specifically removed through capsular depolymerases pretreatment, so that shielding and interference of a polysaccharide layer on MALDI-TOF MS characteristic protein spectrum peaks are overcome. Through the treatment of the method, the problem that a low-confidence-coefficient mass spectrum is generated or a mass spectrum cannot be obtained due to the influence of high-level capsular polysaccharide can be effectively solved, so that the identification confidence coefficient score stably reaches a reportable level. The method disclosed by the invention is simple and convenient in steps and short in treatment time, and solves the problem that the bacteria are difficult to quickly identify through MALDI-TOF MS due to high capsular polysaccharide content.
Owner:DONGGUAN SOUTHEAST CENTRAL HOSPITAL (DONGGUAN SOUTHEAST TRADITIONAL CHINESE MEDICINE MEDICAL SERVICE CENTER DONGGUAN FIRST HOSPITAL AFFILIATED TO GUANGDONG MEDICAL UNIVERSITY)