7 results about "Matrix-assisted laser desorption/ionization" patented technology

Embodiments relate to an apparatus, method, or computer program. A laser may be configured to irradiate a plurality of laser pulses on a target area to ionize a sample placed in the target area into at least one ionized particle. Electrodes at a first end of a flight tube may be configured to accelerate ionized particles into the flight tube. A detector at a second opposite end of the flight tube may independently measure a time of flight of the ionized particles through the flight tube and an intensity of the ionized particles.

Provided is a method for analyzing a neurogranin-related peptide by matrix assisted laser desorption / ionization-mass spectrometry, in which a laser irradiation target contains the neurogranin-related peptide, a matrix, and a protein of 9 kDa or more, and a content of the protein per 1 μg of the matrix is 10 fmol or more and 600 fmol or less.

The present application relates to a kind of nanometer carbon powder-ion type surfactant mixed matrix and its preparation method and application.The mixed matrix includes nanometer carbon powder and surfactant with the molar ratio of (400-600):1.The preparation method of the mixed matrix includes the following steps: according to molar ratio, nanometer carbon powder is dispersed with surfactant in pure water, ultrasonic oscillation, and mixed solution is obtained;After the mixed solution is stirred, centrifugal, and nanometer carbon powder-ion type surfactant mixed matrix solution is obtained.The mixed matrix is applied in matrix assisted laser desorption ionization mass spectrometry detection, specifically including the following steps: mixed matrix coating: take mixed matrix in spray gun, spray on target plate, and dry after spraying;Sample coating: the sample to be measured is coated on the target plate covered with mixed matrix coating, and positive ion mode mass spectrometry analysis is carried out after drying.Compared with prior art, the present application has the advantages of simple preparation, low cost, good stability, can effectively improve peak intensity and signal-to-noise ratio, etc.

1. Name of the designed product: Time-of-flight mass spectrometer. 2. Use of the designed product: Instrument for micro-matrix assisted laser desorption ionization. 3. Design points of the designed product: Combination of shape and pattern. 4. Picture or photograph best indicating the design points: Fig. 1.

A method for analyzing a double-stranded oligonucleotide using an ion trap-type mass spectrometry device provided with an ion source using a matrix-assisted laser desorption ionization method, an ion trapping unit for trapping and discharging ions generated in the ion source, and a detection unit for detecting the ions discharged from the ion trapping unit, a method for analyzing a double-stranded oligonucleotide contained in a sample by introducing a sample into an ion source and irradiating the sample with laser light, capturing ions generated in the ion source in an ion capturing unit, and then discharging and detecting the ions captured by the ion capturing unit, the delay time, which is the time from the irradiation of laser light to the sample by the ion source to the application of a capture voltage for capturing ions to the ion capture unit, is set to be a delay time. The delay time for capturing ions having a mass-to-charge ratio that is the same as the mass-to-charge ratio corresponding to the molecular weight of the double-stranded oligonucleotide is longer than a value that is set in advance for the ion trap-type mass spectrometric analyzer as the delay time for capturing ions having a mass-to-charge ratio that is the same as the mass-to-charge ratio. As a result, a double-stranded oligonucleotide can be analyzed.

The invention discloses a hepatitis B virus infection rapid diagnosis system combining machine learning and peripheral serum MALDI-TOF MS (Matrix Assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry). On the basis of an HBV peripheral blood serum MALDI-TOF MS proteomics database, a LightGBM algorithm is utilized to construct an HBV infection machine learning prediction model, the AUC of the model is 0.88, and the F1 Score is 0.79. The HBVpred performs addition value standardization on multiple pieces of protein fingerprint spectrum data of a single peripheral blood serum sample, calls the models respectively, performs intelligent statistics and outputs a prediction result. Compared with the prior art, the HBVpred system has the advantages of high flux, short time consumption, low cost, high specificity, high identification accuracy, simplicity in operation and the like, and has a huge application prospect.

The invention relates to a method for improving the accuracy of MALDI-TOF MS (Matrix Assisted Laser Desorption / Ionization According to the method, capsular polysaccharide of bacteria is specifically removed through capsular depolymerases pretreatment, so that shielding and interference of a polysaccharide layer on MALDI-TOF MS characteristic protein spectrum peaks are overcome. Through the treatment of the method, the problem that a low-confidence-coefficient mass spectrum is generated or a mass spectrum cannot be obtained due to the influence of high-level capsular polysaccharide can be effectively solved, so that the identification confidence coefficient score stably reaches a reportable level. The method disclosed by the invention is simple and convenient in steps and short in treatment time, and solves the problem that the bacteria are difficult to quickly identify through MALDI-TOF MS due to high capsular polysaccharide content.