The invention relates to the technical field of
analytical chemistry, in particular to a method for quantitatively analyzing BSA (
Bovine Serum Albumin) through SERS (Surface Enhanced
Raman Scattering) based on an AgNR-coated MIP probe and a nano-silver
sol substrate. Comprising the following steps: (1) preparing a BSA standard working
solution system; (2) preparing a blank control
solution system; (3) respectively determining the surface-enhanced
Raman scattering peak intensity value I at 1621.8 cm <-1 > of the BSA standard working
solution system and the blank control solution
system in a portable Raman
spectrometer, simultaneously determining the surface-enhanced
Raman scattering peak intensity value I0 of the blank control solution
system, and calculating
delta I = I-I0; (4) drawing a working curve according to the relationship between the
delta I and the concentration of the BSA; (5) preparing a sample solution according to the method in the step (1), determining the surface enhanced
Raman scattering peak intensity value of the sample solution as Isample, and calculating
delta Isample = Isample minus I0; and (6) calculating the BSA content of the sample solution according to the working curve. The determination method adopts an SERS (Surface Enhanced
Raman Scattering) method in which AgNR-coated MIP is specifically combined with BSA (
Bovine Serum Albumin), and is simple, convenient, rapid and high in sensitivity.