The invention provides an adjustment method of detection equipment, and the detection equipment sequentially comprises an objective lens, a spectroscope, a
barrel lens and a
detector from an object space to an image space, and also comprises a Kohler illumination module. The illumination
light beam is emitted from the Kohler illumination module and is transmitted to the objective lens through the spectroscope, when the object to be detected is arranged on one side, far away from the spectroscope, of the objective lens, the illumination
light beam emitted from the objective lens is reflected to obtain a reflected
light beam, and the reflected light beam sequentially passes through the objective lens, the spectroscope and the
barrel lens to reach the
detector. After an aperture diaphragm is inserted into the Kohler illumination module and an auxiliary lens is arranged between the
barrel lens and the
detector, obtaining a
first light spot position of a
light spot formed by the aperture diaphragm at an
entrance pupil of the objective lens through the detector; and under the condition that the Kohler illumination module does not provide the illumination light beam and the
light source is used as an object to be detected, acquiring a second
light spot position of the
light spot of the
entrance pupil of the objective lens through the detector, adjusting the position of the aperture diaphragm according to the
first light spot position and the second light spot position, and aligning the illumination
optical axis and the imaging
optical axis.