12 results about "Electrospray ionization" patented technology

The invention relates to a sample pretreatment method for desorption electrospray ionization mass spectrometry imaging of a biological tissue frozen section, and the method comprises the following steps: pre-spraying the surface of the frozen biological tissue section, a pre-spraying solvent being a histocompatible solvent, and being used for enhancing the extraction of components in biological tissue cells, the detection sensitivity of components in biological tissues, especially in cells, is improved.

The utility model provides an electrospray ionization device based on ozone gaseous derivative ionization, which comprises a device for supplying ozone to a mass spectrum ion source and the mass spectrum ion source, and the mass spectrum ion source comprises an ion source and an ion inlet device, the apparatus for supplying ozone to a mass spectrum ion source includes an oxygen supply device and an ozone generator, oxygen is supplied into the ozone generator through the oxygen supply device, ozone is generated, and the apparatus for supplying ozone to a mass spectrum ion source supplies the generated ozone to an ion source.

The utility model relates to a transmission type desorption electrospray ionization source which comprises a flow injection pump, a capillary electrospray needle, a capillary high-voltage power supply device, a transmission grid mesh, an ion funnel device and an interface electrode, an ionization area is formed between the capillary electrospray needle and the transmission grid mesh, and an ion convergence area is formed between the transmission grid mesh and the interface electrode. The transmission-type desorption electrospray ionization source can realize high-efficiency desorption electrospray ionization of substances difficult to volatilize in the detection process, the sample detection process is simplified, the detection sensitivity of anesthetic drugs in blood is as low as ng [mu] L <-1 >, the transmission-type desorption electrospray ionization source can be connected with an ion mobility spectrometry, and high-efficiency ionization of high-boiling-point polar chemicals difficult to volatilize is realized.

1. The name of the design product: desorption electrospray ionization imaging source. 2. The use of the design product: a desorption electrospray ionization imaging source, which makes the analyte desorb and ionize by spraying solvent to bombard the sample surface, and is used for mass spectrometric analysis of substances. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention belongs to the field of controllable generation methods of amine aerosol particles and CO2 gas-solid reaction research, and relates to a controllable generation method of high-stability amine nano-particles and application of the high-stability amine nano-particles in CO2 gas-solid reaction research, and the controllable generation method comprises the following steps: 1) putting an amine compound and a sample into an amine aerosol generation device for heating, gas-phase organic matter impurities are removed through an activated carbon decomposing agent, and amine aerosol mixed particles are obtained; (2) introducing the amine aerosol mixed particles obtained in the step (S1) into a double-layer flow tube reaction device to carry out a multi-phase reaction with CO2, introducing a part of products after the reaction into an amine particulate matter particle size and concentration online detection device to carry out detection, and introducing the other part of products into a CO2 adsorbent to remove residual CO2 so as to obtain the product amine aerosol particles; and (3) extracting the product amine aerosol particles obtained in the step (2) into a solvent electrospray, and introducing the product amine aerosol particles into an HRMS on-line detection unit through an ion transmission tube for detection after the product amine aerosol particles are ionized.

An apparatus for ion analysis or an Electrospray Ionisation, ESI, interfacing apparatus comprising a virtual impactor comprising a minor flow part configured for directing towards a downstream region a part of a received flow of a gas entrained with ions as a minor flow of the gas containing at least a majority of the ions. A major flow part is configured for directing away from the downstream region a part of the received flow of gas as a major flow of the gas containing not more than a minority of the ions.

An on-line ionization mass spectrometry method for rapid detection of nicotine and cotinine in urine uses membrane extraction surface pyrolysis desorption electrospray ionization mass spectrometry to perform on-line analysis of nicotine and cotinine in urine. The device involved includes a membrane extraction system, a surface pyrolysis desorption electrospray ionization source, and a mass spectrometer to achieve membrane extraction enrichment of analytes in urine samples, pyrolysis desorption and ionization of analytes, and mass spectrometry detection of analytes. In this method, urine samples are injected into the membrane extraction system, and the components in the urine are extracted and enriched on the membrane. The pre-heated nebulizing gas and electrospray reagent ions perform pyrolysis desorption on the membrane surface, and at the same time complete the release and ionization process of the extracted components. Nicotine and cotinine ions enter the mass spectrometer for analysis. This method can complete the extraction and enrichment of the target substances on-line, and also inhibits the introduction of inorganic salts in urine, overcomes the influence of inorganic salts on the mass spectrometer, combines membrane extraction with surface pyrolysis desorption electrospray ionization mass spectrometry, and can achieve rapid analysis of nicotine and cotinine in urine samples.

The invention generally relates to high-throughput label-free enzymatic bioassays using desorption electrospray ionization-mass spectrometry (DESI-MS).

The present invention discloses a method for identifying and verifying the type of N-linked sugars on glycoproteins, comprising the following steps: preparing a complete N-glycopeptide sample; electrospray ionizing the complete N-glycopeptide sample to obtain precursor ions, gas-phase dissociating the precursor ions to obtain fragment ions, and performing mass spectrometry analysis on the precursor ions and fragment ions; identifying the monosaccharide composition, potential sequence structure, and type of N-linked sugars based on molecular composition fingerprint comparison; screening and confirming the types of N-linked sugar type characteristic fragment ions; false positive control for complete N-glycopeptide identification based on targeted-bait library search; and identification and confirmation of N-linked sugar types. This method identifies and confirms the type of N-linked sugars on glycoproteins based on molecular composition and structural fingerprints, effectively controlling the distinction and confirmation of N-linked sugars of the same molecular composition but different types. It adds a confirmation step based on structural fingerprints to the existing spectrum-level false positive control, thereby improving the accuracy of N-linked sugar type identification.

The invention discloses a single cell mass spectrometry system, which is characterized in that a microscopic optical observation assembly in the system observes a cell detection area in a micro-fluidic chip in real time, and sends out a printing trigger signal when identifying that a single cell flows; after receiving the printing trigger signal, the piezoelectric driving assembly immediately acts on a printing cavity of the micro-fluidic chip to promote the micro-fluidic chip to print single-cell liquid drops; positioning the single-cell liquid drops on the glass slide by the machine vision-assisted alignment system; the glass slide moving mechanism drives the glass slide to move, so that charged fog drops sprayed by the desorption electrospray ionization source can just impact the single-cell liquid drops positioned by the machine vision auxiliary alignment system; the relative positions of the mass spectrometer and the desorption electrospray ionization source are fixed, and single-cell secondary fog drops generated after single-cell liquid drops on the glass slide are ionized by charged fog drops can exactly enter the mass spectrometer to be detected and analyzed. According to the invention, accurate separation and mass spectrometric detection analysis of single cells can be realized on the premise of not depending on markers and avoiding cell damage.

The present invention relates to a detection and analysis method of carotenoid spatial metabolome, and belongs to the field of agricultural chemical component analysis. This application takes sweet corn and pepper as an example, and utilizes desorption electrospray ionization mass spectrometry imaging technology (DESI-MSI), and the spray solvent used is acetonitrile: isopropanol: water: formic acid solution in a volume ratio of (40-60): (30-50): 10: 0.1. The method can intuitively observe the distribution characteristics of carotenoids in plants. By analyzing the response value of the imaging distribution map of the distribution of carotenoid metabolites, the spatial metabolome differences and tissue specificity that different carotenoid metabolites have are obtained, the difference between active metabolites in different tissues is analyzed, and a valuable reference basis is provided for extracting carotenoid metabolites in plants.

An integrated system for liquid separation and electrospray ionization includes an emitter capillary; an LC column; a conductive sheath; a bio-inert conductive union forming a fluidic connection between the emitter capillary and the LC column, the bio-inert conductive union electrically coupled to the conductive sheath, the bio-inert conductive union facilitating the application of high voltage to a mobile phase flowing from the LC column to the emitter capillary; and an insulating plastic material encapsulating the LC column.