40 results about "Electrospray ionization" patented technology

An ion source is disclosed for forming multiply-charged analyte ions from a solid sample. A beam of pulsed radiation is directed onto a portion of the sample to desorb analyte molecules. A retaining structure holding a solvent volume is positioned proximate the sample. Desorbed analyte molecules contact a free surface of the solvent and pass into solution. The solution is then conveyed through an outlet passageway to an electrospray apparatus, which introduces a spray of charged solvent droplets into an ionization chamber.

The invention provides a method used for identifying and analyzing samples. The sample is ionized by two or three combination types out of the following types: photoionization VUV, radioactive <63>Ni ionization, point discharge ionization and electron spray ionization. Subsequently, the detection in a positive ion mode and the detection in a negative ion mode are carried out at the same time, thus obtaining positive/negative ion signal spectrogram; the obtained ion signals are respectively amplified by a micro-current amplifier and subsequently collected by a multi-passage data collecting card; the data is analyzed by a computer so as to obtain the type and the consistency of the sample to be tested. The method has the advantages that according to type difference of compounds measured by ion mobility spectrometry under different ionization sources and different detection modes, the types of the compounds measured by the ion mobility spectrometry is enlarged; meanwhile, according to the difference between the characteristic of the spectrogram and the spectrogram, the sample is analyzed and identified, thus improving the analysis and identification capability of the ion mobility spectrometry on the sample; meanwhile, the advantages the ion mobility spectrometer is small and portable can be kept.

An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.

According to the present invention, there is provided a method of detecting a membrane protein by mass spectrometry, the method comprising the steps of: (a) providing a solution comprising a detergent micelle in which said membrane protein is contained, wherein said solution contains a polyoxyalkylene glycol detergent; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the micelle; (d) ionising the membrane protein; (e) resolving the ionised membrane protein using the mass analyser; and (f) detecting the resolved membrane protein using the detector. Also provided are reagents for use in said method.

A microsecond time-resolved mass spectrometry device and method of using desorption electrospray ionization (10) was described for the kinetic study of fast reactions. The device includes a liquid jet generator (64) that is configured to emit a continuous liquid jet (50) having a length. An ambient ionization source (10) is directed toward a selected variable location along the continuous liquid jet (50) to desorb at least a portion of the continuous liquid jet (50). A mass analyzer (30) analyzes a mass-to-charge ratio of an ionized sample that is within the desorbed portion of the continuous liquid jet (50). The acquired mass spectra reflect the reaction progress in different reaction times and, therefore, may be used to derive the reaction rate.

The present invention relates to a method for preparing Pt thin films using electrospray deposition, more specifically a method for preparing platinum thin film using electrospray deposition, including dissolving a platinum (Pt) precursor in ethanol to prepare a precursor solution for spraying (Step 1); applying a DC voltage between a substrate holder and a nozzle of an electrospraying device and then spraying the precursor solution prepared in Step 1 on a substrate which is maintained at about 100° C. to about 180° C. to form a platinum thin film (Step 2); and subjecting the platinum thin film formed in Step 2 to a heat treatment (Step 3). According to the present invention, the thickness of the platinum thin film may be easily regulated by controlling the amount of a precursor solution sprayed, and the platinum thin film may be continuously prepared on a substrate which has a wide area, therefore a highly active platinum thin film may be obtained by using a small amount of platinum which is expensive, and the film may be usefully used as an Pt electrode used a stable cathode electrode for a process which converts the photo energy into electric or chemical energy, or for a wastewater treatment system or a water purification system.

The invention relates to a photo ionization mass spectrum imaging device combing with desorption electrospray ionization, comprising a mass spectrometer and a desorption electrospray ionization mechanism; the improvement lies in that a photo ionization mechanism is additionally arranged, and comprises a changeover mechanism and a light source mechanism; the light source mechanism is a synchronousradiation light and laser photo ionization mechanism or a vacuum ultraviolet light source mechanism. In detection and analysis, a solvent flows out from the desorption electrospray ionization mechanism to be used for desorbing a compound in a biological tissue on a glass slide, and the desorbed compound enters the photo ionization mechanism, is ionized by synchronous radiation light and laser or vacuum ultraviolet light, and enters mass spectrum to be analyzed and imaged. According to the invention, mass spectrum information is combined with movement of a two-dimensional sample moving platformto realize synchronous imaging of polar and non-polar compounds in the biological tissue, and in combination with the advantage of photo ionization, the device has wide prospects in the aspects of physiological disease, tumor research and the like in the future.

The invention relates to a dasyatis akajei cartilage polypeptide angiogenesis inhibitor, and a rapid preparation method and an application thereof. An amino acid sequence of the angiogenesis inhibitor is Gly-Tyr-Ile-Cys-Pro-Gln-Trp; and the molecular weight of ESI-MS (electrospray ionization mass spectrometry) detection is 865.98Da. The angiogenesis inhibitor is capable of effectively inhibiting angiogenesis of chick chorioallantoic membrane (CAM), and has an obvious inhibiting effect on expression of three pro-angiogenic factors, namely a Lewis lung cancer mice lung cancer tissue-loaded vascular endothelial cell growth factor (VEGF), a basic fibroblast growth factor (bFGF) and a platelet-derived growth factor (PDGF).

The invention discloses a predicting method for impact energy needed for quantifying the content of a traditional Chinese medicine of triterpenoid saponin on the basis of electrospray protonation cracking. The predicting method comprises the steps that (1) medicinal plants adopting the triterpenoid saponin as a main active constituent are selected as representative plants; (2) triterpenoid saponinmodel compounds with representativeness are selected from the representative plants; and (3) the content of the triterpenoid saponin in the medicinal plants is quantified on the basis of electrosprayprotonation cracking, and the needed impact energy is predicted through the following formulas of CE=0.0337*Q1+0.5686 and CE=0.0337*Mw+0.9361, wherein Q1 is equal to [M+H/NH4]+, the linear range of Q1 is 600-1600 Da, R2 is equal to 0.91, the linear range of Mw is 600-1600 Da, and R2 is equal to 0.9096. The CE value obtained through the predicting method and the CE value obtained through a singlefactor investigation method have no significant difference, and it is explained that the predicting method can be applied to detecting of a triple quadrupole mass spectrum.

An impact ionisation spray or electrospray ionisation ion source comprising a nebuliser (30) having a first conduit (11) for providing a liquid sample and a second conduit (10) for providing a nebulisation gas in order to nebulise the liquid sample is disclosed. The first conduit (11) and second conduit (10) are of unitary construction with each other and may be made from glass. The ion source canprovide a consistent and/or predictable spray profile for the nebulised sample.

The present invention relates to a multi-screening method for the detection of a large number of mycotoxins and metabolites in broiler chickens and pigs, the method comprising collecting the blood of broiler chickens and pigs as a dried blood sample, preparing the dried blood sample for analysis and analyzing the prepared dried blood sample by a two-step liquid chromatography-tandem mass spectrometry LC-MS/MS process. In a first LS-MS/MS step, for a given mobile phase for the LC process, the mass spectrometer operates in negative electro-spray ionization mode, and for another mobile phase for the LC process, the mass spectrometer operates in positive electro-spray ionization mode. Such method can advantageously be used for screening and assessing the exposure of pigs or broiler chickens to feed contaminated with mycotoxins. Also, such method can be used for assessing the impact of the addition of mycotoxin detoxifying agents to animal feed.

An integrated proteomics sample preparation device and method for in-gel digestion of proteins and for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device and method of the present invention allow for digestion, desalting and concentration of sample prior to analysis. More specifically, the device in accordance with an embodiment of the present invention includes a plurality of wells in fluid communication with a an outlet or drainage opening containing a three dimensional structure comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. In a preferred embodiment, the wells are configured so as to prevent a sample carrier present in the wells from clogging the outlet when subjected to a driving force such as vacuum. The device also reduces or eliminates overflowing of a well in the event a drain becomes clogged during automated operation.