A method for detecting β-adrenoceptor agonist drugs in animal tissue

A technology of receptor agonist and adrenaline, which is applied in the field of adrenoceptor agonist drugs, can solve problems such as complicated steps and inability to obtain information on the spatial distribution of drug molecules, so as to shorten the time, avoid information loss, and reduce costs Effect

Active Publication Date: 2021-09-07
广东粤器科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The analysis of β-adrenergic receptor agonist drugs by traditional LC-MS method requires pretreatment of tissue samples, which is complicated and the spatial distribution information of drug molecules in tissues cannot be obtained due to tissue processing.

Method used

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  • A method for detecting β-adrenoceptor agonist drugs in animal tissue
  • A method for detecting β-adrenoceptor agonist drugs in animal tissue
  • A method for detecting β-adrenoceptor agonist drugs in animal tissue

Examples

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Embodiment 1

[0026] Example 1: Detection of bambuterol in rat tissue:

[0027] Instrument: high-resolution mass spectrometer SYNAPT G2-Si (WATERS Company) equipped with desorption electrospray ionization ion source (WATERS Company).

[0028] Sample processing: rats were weighed and anesthetized with chloral hydrate (150mg / ml, 0.2ml / 100g); the rats were divided into three groups: 15min, 30min and 45min groups. After 15 minutes, 30 minutes and 45 minutes of administration of bambuterol, the rats were perfused all over the body, and the tissues were taken, and then the tissue samples were frozen and placed on glass slides.

[0029] Standard product preparation: take another blank rat tissue, weigh the tissue weight, add 1 volume of normal saline to grind; make bambuterol into 20μg / ml, 2μg / ml, 200ng / ml and 20ng with normal saline / ml of medicinal solution; take 50 μl of tissue homogenate and place them in 5 ep tubes, then add 50 μl of different concentrations of medicinal solution and normal ...

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Abstract

The invention belongs to the technical field of animal tissue clenbuterol detection, and discloses a method for detecting β-adrenoceptor agonist drugs in animal tissues. The method is easy to operate, can quickly discover the β-adrenergic receptor agonist drugs in animal tissues, and realize the spatial distribution detection of the β-adrenergic receptor agonist drugs in tissue samples. The method comprises the steps of taking animal tissue samples→preparing samples to be tested (including tissue cryosection and preparation of standard products)→desorption electrospray ionization mass spectrometry detection→imaging result analysis.

Description

technical field [0001] The invention belongs to the technical field of animal tissue clenbuterol detection, and in particular relates to a method for detecting β-adrenergic receptor agonist drugs in animal tissues by using desorption electrospray ionization mass spectrometry technology. Background technique [0002] Salbutamol, terbutaline, bambuterol, clenbuterol, etc. belong to the class of β-adrenoceptor agonist drugs, which are used in veterinary medicine and medicine to treat bronchospasm and shock. Studies have shown that this type of drug can promote animal protein accumulation and muscle growth. However, excessive use causes the drug to accumulate in livestock animal tissues, and eating such animal tissues is harmful to human health. The state strictly controls the residues of β-adrenoceptor agonist drugs in livestock tissues. Therefore, the detection of β-adrenergic receptor agonist drugs in animal tissues is particularly important. [0003] The detection of β-adr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N33/15
CPCG01N27/62G01N33/15
Inventor 武杰张睿谭文
Owner 广东粤器科技有限公司
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