35 results about "Terbutaline" patented technology

The present invention discloses a novel compound and method for the treatment of inflammatory autoimmune diseases, for example, rheumatoid arthritis, using α-adrenergic antagonists and β-adrenergic agonists in combination. Treatment of animals, namely humans, with an α-adrenergic antagonist, preferably, phentolamine, and a β-adrenergic agonist, preferably terbutaline, in combination can significantly suppress the joint destruction and inflammation due to disease in these animals.

The invention relates to the field of medicine, and relates to a synthesis and refining method of sulfuric acid terbutaline compound. The structural formula of the compound is as follows: FORMULA; industrially produced hydrochloric acid bambuterol is used as raw material, and then hydrolyzed under alkaline condition to obtain a sulfuric acid terbutaline crude product, the crude product is crystallized in methanol to obtain a refining product with high purity. The reaction condition is mild, the operation is convenient, the total yield is high (about 50%), the environment pollution is less, and the method is suitable for industrial production. The product meets the latest pharmacopoeia criterion in Europe (7.8 version) and the latest pharmacopoeia criterion in the U.S. (36 version).

The invention discloses a particle used for detection of a veterinary drug micromolecule. The particle comprises a receptor particle and a donor particle and is prepared through the following steps: marking carrier protein with a veterinary drug molecule; coupling the veterinary drug molecule-marked veterinary drug with a homogeneous chemiluminescent receptor ball; and preparing a homogeneous chemiluminescent receptor ball coupled with a veterinary drug molecule antibody. The invention further discloses a one-step homogeneous chemiluminescent method for detection of the micromolecule with the particle. The one-step homogeneous chemiluminescent method is used for homogeneous, rapid and high-sensitivity detection of veterinary drug residue and for testing of the contents of frequently used veterinary drug components in feeds, e.g., clenbuterol, ractopamine, salbutamol and terbutaline.

The invention discloses an electrochemical immunosensor capable of detecting various beta-adrenergic receptor stimulants (short for beta-stimulants). The sensor comprises a base electrode, a graphene / chitosan complex membrane and multi-determinant antigens of beta-stimulants, wherein the graphenee / chitosan complex membrane is modified on the surface of the base electrode, and the multi-determinant antigens of the beta-stimulants are fixed on the surface of the graphene / chitosan complex membrane. The multi-determinant antigens fixed on the surface of the sensor are salbutamol-ractopamine-BSA or salbutamol-ractopamine-OVA. The invention also provides an electrochemical immunodetection method for various beta-stimulant residues. The electrochemical immunosensor can be used for detecting six beta-stimulants including clenbuterol, salbutamol, ractopamine, terbutaline, mabuterol and tulobutezol through carrying out cross immune reaction on the beta-stimulants based on wide-spectrum specific antibodies of the beta-stimulants by taking K3[Fe(CN)6] as a probe.

The invention discloses a preparation method for terbutaline hemisulfate. The method comprises the following steps: by adopting 3, 5-resacetophenone as a raw material, performing bromination reaction by directly using a bromination reagent without protecting hydroxide radical, then reducing carbanyl group; condensing with tert-butylamine, finally, forming salt with sulfuric acid, thereby obtaining terbutaline hemisulfate. The method can overcome the defects of deprotection after hydroxide radical protection, usage of various high-risk toxic reagents, long reaction steps and low yield in the present technology.

The invention relates to a five-conjugate testing card for detecting beta-stimulant drugs and a preparation method thereof and belongs to the technical field of beta-adrenergic receptor stimulant detection. A test strip is arranged in a casing of the testing card which is composed of a polyvinyl chloride (PVC) glue board, a sample pad, a colloidal gold combination pad, a coated film and absorbent cotton. A colloidal gold film is a glass cellulose film which contains colloidal gold markers of a clenbuterol monoclonal antibody, a ractopamine monoclonal antibody, a salbutamol monoclonal antibody, a phenylethanolamine A monoclonal antibody and a terbutaline monoclonal antibody. The coated film is a nitrocellulose film, wherein five detection lines T and a quality control line C are arranged on the coated film, and the quality control line C is coated with a rabbit anti-mouse immunoglobulin G (IgG) antibody. The five-conjugate testing card can simultaneously detect out clenbuterol, ractopamine, salbutamol, phenylethanolamine A and terbutaline, and is simple, convenient and fast in method, and accurate in result.

The invention discloses a chemiluminescence enzyme immunoassay detection reagent kit for terbutaline, which comprises a kit body, an ELIAS plate arranged in the kit body and a reagent in the kit body. The reagent kit is characterized in that each hole of the ELIAS plate is enveloped with an envelope antigen, wherein the envelope antigen is produced by coupling the terbutaline and ovalbumin; and the reagent comprises a terbutaline series standard solution, an enzyme labeled goat anti-rabbit antibody, a terbutaline antibody, a luminescent solution, a washing solution, an envelope solution, and a confining solution. The chemiluminescence enzyme immunoassay detection reagent kit has the characteristics of high sensitivity, simplicity, convenience, quickness, and accuracy; compared with the prior colorimetric ELISA method, the sensitivity can be improved by one order of magnitude; and the reagent kit is expected to play an important role in residue detection of the terbutaline in animal food such as milk, animal tissue, and urine sample.

The invention discloses a coupling matter of terbutaline in general formula (I), constituted in coupling by terbutaline hapten and carrier matter generating immunogenicity, which is bovine serum albumin or ovalbumin. Whereinto, n is the number of terbutaline molecules combined with a bovine serum albumin molecule. Said n is integer from 1 to 15. cBSA is activated bovine serum albumin and the range of molecular weight is 66KDa-69KDa. The invention also discloses the preparation method of said coupling matter. Terbutaline is connected with carrier matter generating immunogenicity to combine the coupling matter which can induce animal immune system to generate antibody. By immunizing New Zealand white rabbit the coupling matter of terbutaline in invention prepares antiserum with value of more than 1:50,000. The lowest measuring limitation is 0.3ppb and IC50 is 9.25ppb. The invention is convenient, quick, special and accurate. It provides a foundation for preparing ELISA kit for detecting of terbutaline.

The invention discloses a colloidal gold kit for detecting a beta-stimulant drug. The kit comprises reagent strips and colloidal-gold marker micropores, the reagent strip comprises a base plate, a chromatography membrane, a sample pad arranged at two ends of the chromatography membrane, and an absorbent pad are arranged on the base plate in order, a quality control line C and a detection line arearranged on the chromatography membrane, the line C is coated with a goat-anti-mouse IgG antibody, the detection line comprises a T1 line and a T2 line, the T1 line is a ractopamine detection line, and is coated with a ractopamine antigen, the T2 line is the salbutamol, clenbuterol, brombuterol, tulobuterol, mabuterol, terbutaline, cimbuterol, clorprenaline, and cimaterol detection line, the T2 line is coated with a beta-stimulant antigen, the line C, the T1 line, and the T2 line present three parallel bands which are vertical to a long phase of a test paper strip, the colloidal-gold marker micropores contains a colloidal gold-labeled beta-stimulant polyclonal antibody and a ractopamine monoclonal antibody, by aiming at special objects of raw milk and fresh milk, ten drugs can be detectedat one time, a detection scope is enlarged, leak detection can be prevented, sensitivity is high, and stability is good.

The present invention relates to the field of biotechnology, and discloses an anti-beta receptor agonist cluster-specific monoclonal antibody hybridoma cell line and a monoclonal antibody secreted bythe cell line. According to the present invention, the hybridoma cell line is prepared from salbutamol artificial antigen, and the secreted monoclonal antibody can simultaneously recognize salbutamol,clenbuterol, terbutaline, brombuterol, cimbuterol and other beta-receptor agonists, and has cluster specificity; the immunoaffinity chromatography gel prepared from the monoclonal antibody and the immunoaffinity chromatography gels of other beta-receptor agonists such as ractopamine are mixed as the machine measurement pretreatment purification reagent, a variety of clenbuterol hydrochloride components in pork, pig liver and pig urine samples can be efficiently detected by combining the machine measurement pretreatment purification reagent, HPLC-FLD and LC-MS / MS, and the high accuracy and high sensitivity can be achieved.

The invention provides application of a compound Haqing injection in preparation of an atomization and rectal administration preparation, and a preparation method thereof. The preparation can be a drug combination of the compound Haqing injection and one or more of ambroxol, budesonide, terbutaline, dexamethasone and salbutamol, is mainly used for atomization and rectal administration, and has an obvious curative effect on treatment of respiratory diseases such as upper respiratory infection, capillary bronchus and bronchitis.

The invention relates to a preparation method of high optical purity and high chemical purity levo(-)terbutaline. The method provided by the invention has the advantages of cheap and easily availablebulk drugs, simple operation and short reaction period, is suitable for industrial production, improves the total yield, and at the same time can acquire high optical purity and high chemical purity product. The high optical purity and high chemical purity levo(-)terbutaline provided by the invention is applied to drug efficacy study of resistance to asthma and obstructive pulmonary disease. The result indicates that compared with the commercially available despinners, the levo(-)terbutaline can improve the anti-asthma efficacy manyfold, and proves a new obtains products with high chemicalpurity and optical purity. The anti-asthma and pulmonary obstruction effects of levo-terbutaline with high optical purity and high chemical purity in the present invention were studied. The results show that levoterbutaline in the invention can double the antiasthmatic efficacy compared with racemates sold in the market, and provides a new optimal treatment scheme for asthma related diseases.

The invention discloses an immune-electrochemistry sensor for detecting seven beta-adrenergic receptor agonists, and a manufacture method and application of the immune-electrochemistry sensor. The immune-electrochemistry sensor comprises a substrate glass electrode, and a film layer and a detection layer which sequentially coat the surface of the substrate glass electrode, wherein the material of the detection is a beta-agonist general hapten 4-(4-(2-terttert-butylamino) 1-ethoxy) phenylbutyric acid solution. The method comprises the following steps: firstly dispensing graphene / chitosan dispersion liquid on the surface of the clean substrate glass electrode, drying to form a film formation layer, dispensing the 4-(4-(2-terttert-butylamino) 1-ethoxy) phenylbutyric acid solution on the surface of the film layer, and then drying. The sensor is based on the cross immunoreactions of a beta-agonist broad specificity antibody on various beta-agonists, and can realize the detection of seven beta-agonists including clenbuterol, salbutamol, mabuterol, brombuterol, cimbuterol, tulobuterol and terbutaline.

The invention discloses an electrochemical immunosensor capable of detecting various beta-adrenergic receptor stimulants (short for beta-stimulants). The sensor comprises a base electrode, graphene and multi-determinant antigens of beta-stimulants, wherein the graphene is modified on the surface of the base electrode, and the multi-determinant antigens of the beta-stimulants are fixed on the surface of the graphene. The multi-determinant antigens fixed on the surface of the sensor are clenbuterol-salbutamol-bovine serum albumin (CL-SAL-BSA) or clenbuterol-salbutamol-ovalbumin (CL-SAL-OVA). The invention also provides an electrochemical immunodetection method for various beta-stimulant residues. The electrochemical immunosensor can be used for detecting six beta-stimulants including clenbuterol, salbutamol, ractopamine, terbutaline, mabuterol and tulobutezol through carrying out cross immune reaction on the beta-stimulants based on wide-spectrum specific antibodies of the beta-stimulants by taking K3[Fe(CN)6] as a probe.

The invention relates to a pretreatment method for rapid determination of beta-agonists in livestock and poultry meat through gas chromatography / mass spectrometry and discloses a simple, convenient, highly efficient and accurate pretreatment technology in determination of terbutaline, clenbuterol, salbutamol and ractopamine through gas chromatography / mass spectrometry. According to the method, a minced meat sample is ultrasonically extracted with 5% perchloric acid, then centrifuged at a speed of 10000 r / min for 15 min; then supernatant is enriched and purified with a WCX solid-phase extraction column; and detection is carried out after derivatization. The four agonists have good linearity in a range of 50 to 1000 [mu]g / L; correlation coefficient is more than 0.995; standard-added recovery rate is in a range of 84.32 to 103.15%; RSD is in a range of 1.35 to 4.78%; occurrence of false positive results is prevented; and lowest detection limit and the standard-added recovery rate meet current detection requirements at home and abroad.

The present invention relates to a bambuterol hydrochloride tablet composition, which comprises, by mass, 5-10% of bambuterol hydrochloride, 30-60% of lactose, 10-40% of starch, 10-30% of sodium carboxymethyl starch, 1-3% of talc powder, 0.2-0.8% of magnesium stearate, and 0.2-0.8% of sodium carboxymethyl cellulose. According to the present invention, the plasma half-life is about 17 h after the bambuterol hydrochloride tablet composition is subjected to oral administration, the plasma half-life of the active metabolite terbutaline is about 17 h, the effective action effect of the bambuterol hydrochloride tablet composition can achieve 24 h, the bambuterol hydrochloride tablet composition is only be taken once every night so as to achieve easy administration, the medical values are provided for asthma patients with easy attacking at night or on the morning and aggravation, and the bambuterol hydrochloride tablet composition is an ideal anti-asthmatic drug.

The invention relates to the technical field of preparation and application of chiral high-performance liquid chromatography column materials, and discloses preparation and application of beta-CD-D2 high-performance liquid chromatography chiral column material. A high-performance liquid chromatography chiral column is prepared from beta-CD-D2 and is used for splitting chiral medicine enantiomers. The preparation and the application of the beta-CD-D2 high-performance liquid chromatography chiral column material comprise the following steps: 1, constructing a chiral environment by using beta-CD-D2, bonding the chiral environment with high-performance liquid chromatography silica beads into a fixed phase filler, then preparing the fixed phase filler into a chiral column, and applying the chiral column into high-performance liquid chromatography; 2, finding out an optional formula and an optional condition by using a proper characterization means, and optimizing the method and the condition; 3, establishing a preparation method of a salmeterol single enantiomer by using the prepared beta-CD-D2 chiral column, and splitting a chiral compound by using a cyclodextrins derivative for the first time; and 4, splitting drugs by using the prepared beta-CD-D2 chiral column, and establishing a method for splitting and qualitatively and quantitatively analyzing seven chiral drugs including salmeterol, terbutaline, procaterol, cetirizine, lamivudine, cefuroxime and ceftriaxone.

The invention provides a method for synthesizing terbutaline and bovine serum albumin conjugate. The method comprises the following steps: 1, dissolving bovine serum albumin (BSA) in a phosphoric acid buffering solution (PBS with the pH value of 6.0), adding terbutaline, stirring the terbutaline and the obtained solution to dissolve the terbutaline, dropwise adding a formaldehyde solution, and carrying out a stirring reaction at room temperature for 24 h to obtain a reaction solution; 2, transferring the reaction solution into a dialysis bag, placing the dialysis bag in a beaker provided with distilled water, and carrying out dialysis under a room temperature stirring condition for 3 days; and 3, centrifuging the above obtained dialysis product by a high speed refrigerated centrifuge, collecting the obtained supernatant, and carrying out vacuum freeze drying to obtain dry powder that is the terbutaline and bovine serum albumin conjugate. The terbutaline and bovine serum albumin conjugate is synthesized through one step, and is of great significance to the immunodetection exploitation and the application of a clenbuterol hydrochloride additive in foods.

The present invention discloses a novel compound and method for the treatment of inflammatory autoimmune diseases, for example, rheumatoid arthritis, using α-adrenergic antagonists and β-adrenergic agonists in combination. Treatment of animals, namely humans, with an α-adrenergic antagonist, preferably, phentolamine, and a β-adrenergic agonist, preferably terbutaline, in combination can significantly suppress the joint destruction and inflammation due to disease in these animals.

The invention discloses a magnetic particle chemiluminescence detection kit of terbutaline and a preparation method thereof. The kit includes: acridinium ester markers, magnetic particles coupled with antigens or antibodies, terbutaline calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution. The kit of the invention uses the magnetic separation chemiluminescence technology as the detection means, and simultaneously combines the acridinium ester labeling technology. The direct chemiluminescence method established by the invention has high sensitivity, strong specificity, accuracy and rapidity, short detection time and higher accuracy and repeatability of detection results, and the kit can be applied to various luminescence detection instruments.

The invention relates to the technical field of preparation and application of chiral high-performance liquid chromatography column materials, and discloses preparation and application of beta-CD-D2 high-performance liquid chromatography chiral column material. A high-performance liquid chromatography chiral column is prepared from beta-CD-D2 and is used for splitting chiral medicine enantiomers. The preparation and the application of the beta-CD-D2 high-performance liquid chromatography chiral column material comprise the following steps: 1, constructing a chiral environment by using beta-CD-D2, bonding the chiral environment with high-performance liquid chromatography silica beads into a fixed phase filler, then preparing the fixed phase filler into a chiral column, and applying the chiral column into high-performance liquid chromatography; 2, finding out an optional formula and an optional condition by using a proper characterization means, and optimizing the method and the condition; 3, establishing a preparation method of a salmeterol single enantiomer by using the prepared beta-CD-D2 chiral column, and splitting a chiral compound by using a cyclodextrins derivative for the first time; and 4, splitting drugs by using the prepared beta-CD-D2 chiral column, and establishing a method for splitting and qualitatively and quantitatively analyzing seven chiral drugs including salmeterol, terbutaline, procaterol, cetirizine, lamivudine, cefuroxime and ceftriaxone.

The invention provides a method for separating terbutaline enantiomers in an extracted mode through hydrophobicity phase transferring chirality. Beta-cyclodextrin is used as an extraction agent, the terbutaline enantiomers serve as separated objects, the solubilizing performance of a hydrophobicity phase transferring agent for terbutaline in the organic phase is used, mass transferring efficiency is improved through the centrifugal acting force of a centrifugal extractor, mass transferring and reacting of the terbutaline enantiomers in the water phase and the organic phase are accelerated, extract-phase outlet purity and raffinate-phase outlet purity are greatly improved accordingly, and the extract-phase yield and the raffinate-phase yield are greatly increased accordingly. The method has the advantages that the extraction agent and the phase transferring agent are low in price and easy to get; extraction efficiency is high, and the separating speed is high; operation is flexible, and the environment-friendly effect and the efficient effect are achieved. By means of the method, the problem that the solubility of the terbutaline in the organic phase is poor is solved, and the problems that as for common extraction technologies, mass transferring efficiency is low, single-stage extraction is carried out, and purity and the yield are low are solved. Rapid and high-selectivity separation of the terbutaline can be achieved through multi-stage counter-current extraction.

The invention discloses a terbutaline, salbutamol, ractopamine, clenbuterol online immunoaffinity purification detecting device. Automatic sample loading and elution of immunoaffinity columns are realized through flow switching of three six-way valves; secondary enrichment purification and desalination are realized by the use of MCX columns; a purification solution can be directly introduced intoa tandem mass spectrometry detector, therefore, high-sensitivity detection of the four drugs based on automatic purification is realized. The immunoaffinity purification columns of the terbutaline, salbutamol, ractopamine, clenbuterol online immunoaffinity purification detecting device can be reused, and the automation of sample purification and analysis are realized, which can save money, save time, and save manpower; and the practicability is good.

This invention disclosed new use of R-terbutaline and other R-enantiomer [beta]2-agonists as immune-modulators for treatment of bronchia-lung inflammatory symptoms or inflammatory fibrosis remolding. This invention also disclosed new use of R-terbutaline and R-[beta]2-agonists for reduced adverse effects related to racemic or S-enantiomer [beta]2-agonists including airway hyper responsiveness and airway fibrosis.

The invention discloses an immune-electrochemistry sensor for detecting seven beta-adrenergic receptor agonists, and a manufacture method and application of the immune-electrochemistry sensor. The immune-electrochemistry sensor comprises a substrate glass electrode, and a film layer and a detection layer which sequentially coat the surface of the substrate glass electrode, wherein the material of the detection is a beta-agonist general hapten 4-(4-(2-terttert-butylamino) 1-ethoxy) phenylbutyric acid solution. The method comprises the following steps: firstly dispensing graphene / chitosan dispersion liquid on the surface of the clean substrate glass electrode, drying to form a film formation layer, dispensing the 4-(4-(2-terttert-butylamino) 1-ethoxy) phenylbutyric acid solution on the surface of the film layer, and then drying. The sensor is based on the cross immunoreactions of a beta-agonist broad specificity antibody on various beta-agonists, and can realize the detection of seven beta-agonists including clenbuterol, salbutamol, mabuterol, brombuterol, cimbuterol, tulobuterol and terbutaline.

The invention relates to the field of biotechnology, and discloses an anti-β-receptor agonist cluster-specific monoclonal antibody hybridoma cell line and the monoclonal antibody secreted therefrom. The hybridoma cell line of the present invention is prepared from salbutamol artificial antigen, and the monoclonal antibody secreted by it can simultaneously recognize salbutamol, clenbuterol hydrochloride, terbutaline, bromobuterol and sibuterol and other β-receptor agonists, Has cluster specificity; the immunoaffinity chromatography gel prepared from this is mixed with other β‑receptor agonists, such as ractopamine, as a purification reagent for machine test pretreatment and HPLC‑FLD, LC‑MS / The combination of MS detection technology can efficiently detect various clenbuterol components in pork, pig liver and pig urine samples, with high accuracy and sensitivity.

The invention discloses a nine-link detection card for beta-incitant. The nine-link detection card comprises a shell and a detection card arranged in the shell, wherein the shell comprises an upper shell and a lower shell; a detection card accommodating cavity is formed between the upper shell and the lower shell; four corners of the lower shell are provided with bosses; the bosses are provided with clamping holes; the upper shell is provided with clamping posts corresponding to the bosses, and the clamping posts are matched with the clamping holes in the bosses; the detection card comprises a bottom plate; the middle of the top of the bottom plate is provided with nine reaction films; one side of each reaction film is provided with a sample pad and a structure release pad, and the other side of each reaction film is provided with a water absorption pad; the tops of the sample pads, the structure release pads and the water absorption pads are provided with fixing films; the parts, corresponding to the middles of the sample pads, of the fixing films are provided with sample feeding holes; nine sample pads and nine reaction films are arranged; the nine sample pads are respectively used for detecting the clenbuterol hydrochloride, ractopamine, salbutamol, cimbuterol, mabuterol, bombuterol, tulobuterol, terbutaline and clorprenaline in the beta-incitant; positions corresponding to the reaction films are observation areas.

The invention provides a method for synthesizing terbutaline intermediates. The method provided by the invention adopts a single solvent for a reaction, the single solvent can be recycled, the processis simplified, and the post-treatment operation is simple. In key steps, dibromohyne is adopted as a brominating reagent, so that the method is green, environment-friendly and pollution-free, and theobtained product is high in purity and considerable in yield.

The invention discloses an atomized salbutamol solution for treating respiratory diseases and a preparation method thereof and relates to the field of atomized salbutamol solution preparation. The atomized salbutamol solution is prepared from, by weight, 45.0-54.5 parts of salbutamol, 35.2-48.0 parts of pH regulator, 15.6-24.0 parts of budesonide, 14.3-18.5 parts of terbutaline, 20.6-32.0 parts ofvitamin and 85.6-96.5 parts of purified water. Compared with the prior art, the atomized salbutamol solution has the advantages that by adopting budesonide, terbutaline and vitamin, repair and protection of intrapulmonary mucosa are realized without impact on respiratory disease treatment; the salbutamol atomized inhalation solution has excellent stability, is little in related substance contentand simple in composition, risk of clinical medication is lowered, medication safety is improved, and the atomized salbutamol solution is simple in preparation process and has wide market prospect.