169 results about "Tissue homogenate" patented technology

This document describes an optical sensor unit and a procedure for the specific detection and identification of biomolecules at high sensitivity in real fluids and tissue homogenates. High detection limits are reached by the combination of i) label-free integrated optical detection of molecular interactions, ii) the use of specific bioconstituents for sensitive detection and iii) planar optical transducer surfaces appropriately engineered for suppression of non-specific binding, internal referencing and calibration. Applications include the detection of prion proteins and identification of those biomolecules which non-covalently interact with surface immobilized prion proteins and are intrinsically involved in the cause of prion related disease.

The present invention provides sample collection, shipping, and storage devices and methods of using the same. These devices and methods are useful, for example, for collecting, shipping, and storing biological samples, such as blood, serum, buccal samples, tissue homogenates, or cell lysates in a dry state. The devices and methods facilitate the rapid drying of biological samples collected on the devices, thereby improving the quality of the stored sample, particularly the protein and small molecule components of the stored sample. The present invention further provides methods of recovering biological samples from such devices.

Methods and systems for the use of Surface Enhanced Raman Scattering nanotags (SERS nanotags) to create homogeneous (no-wash), heterogeneous or sequence detection assay platforms. In certain embodiments the SERS nanotags are used in combination with magnetic particles. Multiplexed assay platforms are also disclosed. In certain embodiments, the assay is useful for clinical proteomics. Assay platforms suitable for use within a biological matrix, for example within whole blood or serum are also disclosed. The assay formats described herein may be used to detect any analyte of interest including but not limited to the detection of cells, viruses, bacteria, proteins, DNA, RNA, or small molecules in any type of biological (animal or plant kingdom) or environmental samples including but not limited to whole blood or serum, occult samples, urine, feces, air, drinking water, phage, any organism, multicellular clumps of cells, for example, cancer tissue homogenate.

The invention discloses albumen powder richening in antimicrobial peptide fly maggot, a preparation method for the albumen powder and a feed additive of the albumen powder. The albumen powder richening in the antimicrobial peptide fly maggot is prepared by a method comprising the following steps of: (1) feeding flies, luring the flies to lay eggs and feeding larva by using feed after incubation; (2) conducting ultrasound induction on the larva; (3) continuously feeding, collecting the larva, washing, conducting disinfection treatment, and conducting tissue homogenate smashing; and (4) filtering, treating filtrate at 0-5 DEG C, removing surface lipid compositions, drying to obtain the albumen powder; adding auxiliary materials in the albumen powder, pelletizing to obtain the feed additive. A ultrasound stimulation mode under specified conditions is adopted to induce the larva to enable an antimicrobial peptide active ingredient content in a body of a housefly to obviously improve, and the feed additive has certain growth promotion and disease-resistant strengthening effects on litopenaeus vannamei, crucian and the like.

The invention discloses a cryopreservation method for a tissue block used for cell culture. The method includes: firstly performing homogenate treatment on a tissue into a chyle state, then adding a refrigerating fluid and mixing them uniformly, subpackaging the mixture into a freezing tube containing sucrose, DMSO and glutathione, then placing the freezing tube in a cryopreservation box and letting the box stay overnight in a -80DEG ultra low temperature refrigerator, and finally putting the freezing tube into liquid nitrogen for long-term preservation. During unfreezing, centrifugal washing by an unfreezing solution and a culture solution is performed in order, then the tissue block can be directly subjected to inoculated culture or can be digested into a single cell and is then subjected to culture. The method provided in the invention firstly employs a homogenizer to treat the tissue into a chyle state, thus facilitating permeation of a cryoprotectant into tissue cells and removal of intracellular water. At the same time, the impermeable protective agent sucrose added in the refrigerating fluid is also conducive to removal the water from the tissue cells. The adding of the antioxidant substance glutathione helps alleviate the damage of oxygen free radicals on intracellular protein. After unfreezing, the tissue block is directly inoculated in a culture bottle, and the adherent survival rate is over 96%.

The invention relates to a method for detecting hyaluronic acid in human serum, body fluid or tissue homogenate by a radioimmunoassay method. By a method of using solid phase-coated and europium-marked hyaluronic acid binding protein (HABP-Eu<3+>), reagents such as a first antibody, a second antibody and the like are not needed, a sample is easy to add, reaction is quick, the reagents have long shelf life and are environmental-friendly, europium is stably combined with HABP, storage time can be more than 12 months, and radioactive pollution is avoided.

Relating to the fields of biotechnologies and cosmetic science, the invention provides a technology for using the umbilical cords and placentas of volunteers to prepare a skin care product for skin rejuvenation. Specifically, the umbilical cords and placentas of term delivery volunteers are separated, blood vessels and envelopes therein are removed, a tissue homogenizing machine is employed to make the obtained tissue into homogenate, and then medical grade glycerin, preservative and the like are added to prepare the skin care product. Being rich in trophic factors, the skin care product has the efficacy of promoting the proliferation of epidermal stem cells and hair follicle stem cells, plays an important role in maintaining skin elasticity and health, and has the advantages of no toxic, side effect or adverse reactions, etc. The umbilical placental skin care product provides a good method for delaying aging and restoring skin elasticity.

The present invention relates to methods and processes of inactivating viral contaminants in a biological source material (e.g. a host cell, cell supernatant, cell lysate, blood plasma, tissue homogenate, or other biological materials) with a solution containing one or more alkylamine compounds. In a particular embodiment, the active ingredients are amphipathic, charged amines or amine oxides coupled to saturated hydrocarbon chains of varying lengths.

The invention discloses a homogenate breaking and injecting device. The device comprises an injector and a breaker; the injector comprises an injection cylinder, the front end of the injector is provided with a push rod with a rubber plug; the breaker comprises a motor, a stirring shaft connected with the motor in a driving way and a blade arranged at the end part of the stirring shaft; another end of the push rod is of a cylindrical shape, the motor is arranged in the cylindrical push rod, the blade is arranged adjacent to the inside end plane of the rubber plug and the stirring shaft penetrates through the middle part of the rubber plug and is fixedly connected with the blade. The injector and the breaker are beneficially combined so that parathyroid glands can be completely and uniformly broken in the injector, therefore the technical problem that an existing tissue homogenate breaker is unlikely to be broken can be solved, and the step of sucking the parathyroid gland homogenate broken cannot be broken, so that the parathyroid gland homogenate can be directly injected to a receptor by the homogenate breaking and injecting device.

The invention provides an earthworm antimicrobial peptide biological pesticide and application and belongs to the field of biochemistry. A technical scheme is as below: extracting earthworm antimicrobial peptide from earthworm body cavity and tissue homogenate by a phosphate buffer; centrifuging a leaching liquor; carrying out ultrafiltration; and concentrating to prepare the earthworm antimicrobial peptide biological pesticide. The biological pesticide of the invention can be effectively applied to control of insect diseases of paddy rice, fruit and vegetable; and the production technology is simple and without generating pollution. The biological pesticide of the invention belongs to a green environment friendly product without pollution, and is especially suitable for production of nuisance-free organic agricultural products.

The invention discloses a solid-phase extracting column, comprising a glass column and a filler. The filler is hydroxypropyl sephadex LH-20. The solid-phase extracting column can be used for pretreatment of biological samples, in particular used for simultaneous concentration and analysis detection of middle and small molecules in serum, urine and tissue homogenate and the molecular weight of middle and small molecules is close. The solid-phase extracting column in the invention can suppress interference effectively, ensure high sensitivity, good specificity and high accuracy of analysis of analytes and has excellent guiding significance to organism metabonomics research and detection of disease. Furthermore the solid-phase extracting column in the invention can be used repeatedly and hardly exist the phenomenon of regeneration. The extracting column is easy in filling, simple in operation. The column has stronger application value in analysis of biological samples.

Provided herein are methods of recovering single nuclei from a tissue sample comprising chopping or dounce homogenizing the tissue sample in a nuclear extraction buffer at 4° C. to produce a tissue homogenate; centrifuging the tissue homogenate to produce a nuclear pellet; resuspending the nuclear pellet in a nuclear resuspension buffer comprising bovine serum albumin, RNase inhibitor, and salts to produce a resuspension; and filtering the resuspension through a strainer, wherein the single nuclei are present in a supernatant passed through the strainer. The invention also provides a method of single cell sequencing comprising extracting nuclei from a population of cells under conditions that preserve a portion of the outer nuclear envelope and rough endoplasmic reticulum; sorting single nuclei into separate reaction vessels; extracting RNA from the single nuclei; generating a cDNA library, whereby gene expression data from single cells are obtained. The tissue sample may be fresh or frozen.

The invention discloses a preparation method of an iridovirus cell inactivated vaccine for Chinese giant salamanders, and an application method thereof. The vaccine is prepared by the following method: 1, sterilely acquiring the liver of a moribund giant salamander containing frog iridovirus, preparing a tissue homogenate; 2, quickly cracking the tissue homogenate by ultrasonic wave of 20kHz on an ice surface, filtering; 3, centrifugally separating the filtrate to obtain pure virus precipitate, then regulating the virus titer to 105TCID50; 4, culturing the virus cells, collecting a cell culture solution; and 5, and inactivating the cell culture solution by formaldehyde with the final concentration of 0.4% at 37 DEG C for 5-10 hours, thus obtaining the iridovirus cell inactivated vaccine for Chinese giant salamanders. The vaccine is effective when preserved at 4 DEG C within one year. For young giant salamanders and adult giant salamanders below 0.25kg in body weight, dipping bath with the dilute solution of the vaccine is adopted for immunization, and for adult giant salamanders above 0.25kg in body weight, intramuscular injection is adopted for immunization. The vaccine has very strong immunologic specificity, and the immunization rate can reach 80-93%.

The invention discloses solid-phase extraction filler and a preparation method thereof. The filler is filler ODS-CD-HPS bonding beta-cyclodextrin and octadecyl silane mixing functional groups. The invention further discloses a solid-phase extraction column filled with the filler ODS-CD-HPS. The solid-phase extraction column can be applied to pretreatment of biological samples and can be particularly applied to enrichment and analytical detection of medium-polarity and weak-polarity matter in body fluid and tissue homogenate of human bodies. Compared with an existing common solid-phase extraction column based on amorphous silica gel filler, the solid-phase extraction column only needs to be leached with an organic solvent with a small volume, and the extraction recycle rate, reproducibility and extraction efficiency of the medium-polarity and weak-polarity matter are high. Besides, when the solid-phase extraction column is used for pretreatment, leaching and concentrating are completed at a time, and the obtained leached liquid can be enriched and concentrated without extra heating, nitrogen blowing and other steps.

The invention discloses a mussel glycoprotein and a preparation method and an application thereof. The mussel glycoprotein disclosed by the invention is prepared by the following steps: carrying out tissue homogenization of mussel soft tissues; extracting by a buffer solution; carrying out ion exchange column chromatography; carrying out gel column chromatography; purifying by virtue of a fast protein purifying system; concentrating; and freeze-drying. The molecular weight of the mussel glycoprotein is about 16.9kDa and the content of sugar and proteins is respectively 23.54% and 76.46%. The mussel glycoprotein contains O-glucosidic bonds and N-glucosidic bonds. Monosaccharide consists of D-glucose, L-arabinose, D-galactose, D-fructose and D-mannose in a corresponding proportion of 1.00 to 2.01 to 6.93 to 7.27 to 4.05. The protein consists of 15 amino acids. The mussel glycoprotein is remarkable in activity, can be clinically used as an agiogenesis inhibitor and can be used for treating tumor diseases such as prostatic cancer.

The invention provides a cowhide processing method for preparing collagen, and belongs to the technical field of biological food. The method is used for performing fine processing on cowhide before collagen preparation, and the cowhide serves as the main raw materials for preparing the collagen. The method comprises the steps that cow dermis tissue is unfrozen, wherein the frozen cow dermis tissue is placed into a beaker and soaked by adding ultrapure water of which the volume is four times of that of the cow dermis tissue until the cow dermis tissue is unfrozen into loosen tissue particles, and the tissue particles are evenly stirred with a glass rod into paste; the cow dermis tissue particles are mashed into even suspension liquid free of obvious cowhide particles through a tissue masher; the pasty tissue particles are transferred to a glass cup of the tissue masher, ten times volume of ultrapure water is added, homogenization is performed for three times according to the operating instruction of the tissue masher until homogenate is completely suspended, and the tissue homogenate is transferred to a plastic drum; glacial acetic acid is added into the tissue suspension plastic drum to regulate the concentration of the solution to be 0.5 milligram/milliliter. According to the method, the pasty liquid obtained by processing the cowhide is used for collagen extraction, and therefore the purification rate and purity of the collagen can be obviously improved.

The invention relates to a method for preparing gram-level high-purity natural thiosulfinate, belonging to the technical field of compound preparation. The method comprises the following steps: preparing an allium tissue homogenate containing active alliinase, keeping the temperature, washing off endogenous thiosulfinate generated in the tissue homogenate with an organic solvent, preparing exogenous ACSO into a solution, adding the solution into the tissue homogenate, keeping the temperature to generate single thiosulfinate or mixed thiosulfinates, and finally, purifying to obtain the product. The method has the advantages of mild reaction conditions, high product purity, high reaction yield, high cleanness and environmental protection, is simple to operate, and conforms to green technological requirements. The purity of the prepared pure thiosulfinate is sufficient for manufacturing drugs and especially diallyl thiosulfinate (garlicin). The research indicates that the garlicin has the maximum antibacterial, antihypertensive, anticancer, antioxidation and other activities in thiosulfinate.

The invention provides preparation methods of sheep placenta polypeptide powder and soluble granules. The preparation method of sheep placenta polypeptide powder comprises the following steps: achieving pretreatment to the sheep placenta raw material and realizing tissue homogenate; adding protease and flavourzyme into the homogenate for enzymolysis, deodorization, debittering and enzyme deactivation; conducting centrifugal edulcoration to obtain a sheep placenta polypeptide solution; concentrating and drying to obtain sheep placenta polypeptide dry powder. The preparation method of the soluble granules comprises the step of mixing the sheep placenta polypeptide dry powder and synanthrin or xylose at the proportion of 1:2 to prepare granules. The sheep placenta polypeptide is prepared into soluble granules through adding xylose, synanthrin and the like, so that the water absorbability of the sheep placenta polypeptide powder is reduced, the water solubility of the sheep placenta polypeptide powder is improved and the product flavor is effectively improved. The granules are rich in nutritions, easy to absorb and excellent in flavor and taste, and has the anti-fatigue, immunoregulation, beauty maintaining and young keeping functions. The preparation methods are reasonable in process, simple in manufacture and strong in operability and are suitable for mass production.