52 results about "Tissue homogenate" patented technology

The invention belongs to the technical field of biology, and particularly relates to an extraction method of polydeoxyribonucleotide, which comprises the following steps: carrying out homogenate treatment on pretreated in-vitro testis tissue, filtering the obtained homogenate, adding a pancreas extract into the filtrate, and stirring to obtain enzymatic hydrolysate; and precipitating protein in the enzymatic hydrolysate, removing the protein, adding sodium acetate for treatment, adding absolute ethyl alcohol for precipitation, centrifuging, washing the obtained precipitate, and drying to obtain the product. Common trypsin and lipase in fish pancreas are beneficial to release of DNA, the fish pancreas extract is applied to extraction of the polydeoxyribonucleotide, the extraction time can be remarkably shortened, the yield can be increased, meanwhile, nuclease in the fish pancreas extract can effectively degrade DNA, and small-fragment polydeoxyribonucleotide is obtained.

Provided in the present invention is an analysis and detection method for simultaneously detecting tretinoin and tranexamic acid, and the use of the analysis and detection method. The method comprises: analyzing a sample to be tested comprising tretinoin and tranexamic acid by using an ultra-high performance liquid chromatography-triple quadrupole mass spectrum, so as to achieve the qualitative and quantitative detection of tretinoin and tranexamic acid. The analysis and detection method provided in the present invention has the characteristics of a high sensitivity and high precision, good repeatability, high accuracy, high stability, etc. The detection method of the present invention can be relatively easily transferred and applied to the detection of the two compounds in biological samples such as plasma and tissue homogenates, and can satisfy the requirements for drug concentration monitoring.

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

The invention discloses a preparation method of a dairy cow placenta extract CP1 and application of the dairy cow placenta extract CP1 as an antioxidant substance. The preparation method comprises the following steps: taking normal delivery healthy Chinese Holstein dairy cow placenta homogenate as a substrate, shearing the dairy cow placenta into pieces, homogenizing by adopting a tissue homogenizer, adding papain, and acting for 4.7 hours under the conditions that the pH is 6.5 and the temperature is 55 DEG C; the antioxidative peptide has the advantages that the antioxidative peptide with five potential types is screened by the aid of virtual screening processes after the antioxidative peptide is inactivated in boiling water bath for 10 minutes, affinity of the antioxidative peptide with Keap1 after molecular docking is higher than that of TX6, the affinity of ANNGKQWAEVF with Keap1 is the strongest, accordingly, the antioxidative peptide is marked as CP1, the CP1 is excellent in free radical scavenging capacity, lipid peroxidation inhibition capacity, transition metal chelating capacity and reducing power, and the antioxidative peptide with the antioxidative peptide can be used for preparing antioxidative peptide products. In addition, a Keap1 / Nrf2 pathway in oxidative stress cells can be activated, so that the oxidative stress of the cells is relieved, and the compound can be applied as an antioxidant substance.

The invention provides a preparation method of a terminal-peptide-free bovine type I collagen product. The preparation method comprises the following steps: obtaining a crushed bovine tissue raw material without impurities; the preparation method comprises the following steps: soaking a cattle tissue raw material in acid, and homogenizing to obtain cattle tissue homogenate; protease is added into the bovine tissue homogenate in batches for enzymolysis, the enzymolysis temperature is controlled to be 2-6 DEG C, after salting-out precipitation, pH value adjustment and redissolution are carried out on the bovine type I collagen extracting solution obtained in the middle batch, protease is added again for enzymolysis, salting-out precipitation, pH value adjustment and redissolution are carried out again, and the bovine type I collagen extracting solution is obtained. Repeating for multiple times to obtain a terminal-peptide-free bovine type I collagen solution; adjusting the pH value of the terminal-peptide-free bovine type I collagen solution, salting out, precipitating, centrifuging and washing to obtain terminal-peptide-free bovine type I collagen precipitate; and purifying the terminal-peptide-free bovine type I collagen precipitate to obtain the terminal-peptide-free bovine type I collagen product. According to the telopeptide-free bovine type I collagen product provided by the invention, no residual telopeptide is detected, and the purity is high.

The invention provides an analysis and detection method for simultaneously detecting tretinoin and tranexamic acid and application thereof, and the method comprises the following steps: analyzing a to-be-detected sample containing tretinoin and tranexamic acid by using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry to realize qualitative and quantitative detection of tretinoin and tranexamic acid. The analysis and detection method provided by the invention has the characteristics of high sensitivity and precision, good repeatability, high accuracy, strong stability and the like. The detection method disclosed by the invention can be easily transferred and applied to detection of two compounds in biological samples such as plasma and tissue homogenate, and can meet the requirement on drug concentration monitoring.

The utility model discloses a cell tissue homogenizer, which belongs to the technical field of cell tissue homogenization and comprises a placing table, an electric push rod is fixedly connected to the upper surface of the placing table, a support plate is fixedly connected to one end, far away from the placing table, of the electric push rod, and a support column is mounted in the support plate through a bearing. A plurality of gears are fixedly connected to the top ends of the supporting columns, every two gears close to each other are connected in a meshed mode, the upper surface of one gear is fixedly connected with the output end of a servo motor, a mounting base is fixedly connected to the bottom ends of the supporting columns, and a connector is inserted into the mounting base. The lower surface of the connector is fixedly connected with a tamping rod, and the outer surface of the tamping rod is provided with a sealing cover through a sealing bearing. According to the cell tissue homogenizer, cell tissues in the plurality of homogenizing pipes can be homogenized by controlling the plurality of tamping rods to rotate, the homogenizing efficiency is higher, meanwhile, the homogenate is prevented from splashing, and the accuracy of an experimental result is ensured.

The invention discloses an application of cow placenta polypeptide in preparation of a medicine for protecting intestinal injury caused by cyclophosphamide, which comprises the following steps: cutting the placenta of a healthy Chinese Holstein cow in normal delivery into pieces, homogenizing by using a tissue homogenizer, adding papain, acting for 4.7 hours under the conditions that the pH value is 6.5 and the temperature is 55 DEG C, centrifuging after inactivating for 10 minutes in a boiling water bath, collecting the supernatant, and performing freeze-drying to obtain the cow placenta polypeptide. The dairy cow placenta polypeptide ANNGKQWAEVF (H-Ala-Asn-Asn-Gly-Lys-Gln-Trp-Ala-Glu-Val-Phe-OH) is screened through a virtual screening method and molecular docking after freeze drying, the affinity of the dairy cow placenta polypeptide ANNGKQWAEVF (H-Ala-Asn-Asn-Gly-Lys-Gln-Trp-Ala-Glu-Val-OH) and Keap1 is the strongest, the molecular formula of the dairy cow placenta polypeptide is C57H82N16O17, and the molecular weight of the dairy cow placenta polypeptide is 1263.38 Da. The dairy cow placenta polypeptide can improve the villus form of the Release of intestinal barrier related factors is regulated, and expression of intestinal barrier genes is up-regulated.

The invention is applicable to the field of food processing, and provides a preparation method of pig brain oligopeptide capable of promoting learning ability, which comprises the following steps: selecting qualified pig brain subjected to inspection and quarantine, adding clear water, stirring to prepare homogenate, heating to 90-95 DEG C, and keeping for 15 minutes; cooling the obtained tissue homogenate, and carrying out first enzymolysis; adjusting the pH value of the first enzymolysis system, standing and precipitating, separating supernate and precipitate, and adding clear water into the precipitate to re-homogenate; carrying out secondary enzymolysis on the precipitate homogenate; adjusting the pH value of the second enzymolysis system, standing and precipitating, and separating supernate and precipitate; and combining the supernatant obtained by the first enzymolysis with the supernatant obtained by the second enzymolysis, concentrating, dialyzing and drying to obtain the pig brain oligopeptide. The method is simple and convenient to operate, low in cost and high in raw material utilization rate, and the obtained product is high in purity and easy to absorb, has the physiological functions of promoting learning and memory, tonifying and strengthening the brain and the like, and has wide industrial application prospects.

The invention discloses a tissue homogenizer for bulb vegetable detection, and relates to the technical field of food detection.The tissue homogenizer comprises a machine base and a limiting ring, the top end of the machine base is fixedly connected with the limiting ring, a lower cylinder is placed on the inner side of the limiting ring, the top end of the lower cylinder is spirally connected with an upper cylinder, and the top end of the upper cylinder is spirally connected with an end cover; a connecting shaft is rotatably connected to the inner side of the end cover, a source restraining mechanism and a defoaming mechanism are arranged on the outer side of the connecting shaft, the source restraining mechanism is arranged to forcibly press surface slurry (the area close to the liquid level) to the bottom of the cavity, and the problems that a traditional cutter swings the slurry outwards and impacts the liquid level are solved through the design; cutting off the path of air involved into the slurry from the source; and the slurry smoothly and downwards flows along the inclined surface of the cutter without rolling and air entrapment phenomena, so that bubbles generated by turbulent flow are eliminated from the source.

1. Name of the product of this design: Automated extraction equipment for purification and concentration of exosomes. 2. Purpose of the product of this design: This device is used to extract, purify and concentrate exosomes from cell culture supernatant and tissue homogenate. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention discloses a method for extracting cell nucleuses from animal tissues, and relates to the technical field of biology. The background technology indicates that cell nucleus extraction is crucial to multidisciplinary research, but a traditional method has the problems of complex operation, time consumption, low purity, easiness in damaging cell nucleuses and the like, and the prior art is still limited although the prior art is improved. The method comprises the following steps: preparing a sample, and taking fresh or cryopreserved animal tissues; homogenizing the tissues, namely putting the tissues into a homogenizer, and homogenizing at a low speed by adding a cracking buffer solution; carrying out cell lysis, and incubating homogenate on ice; centrifugally separating, centrifuging at low temperature and medium speed, taking precipitate, and discarding supernatant; core washing, re-suspending the precipitate in a washing buffer solution, uniformly mixing and filtering; performing core extraction, re-suspending the precipitate in a suspension buffer solution, and lightly and uniformly mixing; purifying and washing, carrying out density gradient centrifugation on the suspension, dyeing with trypan blue, taking a core layer, and washing for subsequent experiments. The nuclear extraction lysis buffer solution has two proportions of LB1 and LB2, different tissues are suitable for different proportions, and an enzyme inhibitor can be added as required. The method can be used for rapidly extracting various animal tissue cell nucleuses, is complete in shape, large in quantity and few in impurities, is suitable for different tissues, and can be applied to downstream experiments such as single cell nucleus sequencing.

The invention discloses a rapid drug sensitivity detection method based on fish tissue homogenate, which comprises the following steps: taking fish tissues for homogenate, coating and inoculating the obtained homogenate on a flat plate, placing a drug sensitivity paper sheet, culturing for 16-24 hours at 30 DEG C, then measuring the diameter of an inhibition zone on the flat plate, and judging a drug sensitivity detection result. According to the method, the sample pretreatment and detection process is simplified, diseased fish tissue homogenate is directly used, the detection period is remarkably shortened to be within 1 d, and meanwhile the result accuracy is ensured. According to the technical scheme, the method comprises the steps of sample collection under the sterile condition, tissue homogenate preparation, homogenate coating inoculation, drug sensitive paper sheet placement and culture result interpretation. The method has the advantages of high efficiency, rapidness, simplicity and convenience in operation, accurate result and wide application range, is suitable for drug sensitivity detection of various fish pathogenic bacteria, and has important practical application value.

The invention belongs to the technical field of disease control in aquaculture, and particularly relates to a hippocampus enteritis pathogen separation and identification and medication screening method based on a high-throughput sequencing technology, which comprises the following steps: collecting intestinal tissues of a diseased hippocampus and a healthy hippocampus, respectively preparing tissue homogenate, and freezing for preservation; the method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of microorganisms of an intestinal tissue sample, performing 16SrDNA high-throughput sequencing, analyzing intestinal flora composition, and determining dominant bacteria; selecting and screening a culture medium according to dominant bacteria, and performing pathogenic bacterium separation and purification on intestinal tissues of the diseased hippocampus; dNA of the purified strain is extracted, 16S rDNA amplification and sequencing are carried out, and the species of pathogenic bacteria are identified through sequence comparison; according to the method, the diversity and abundance differences of microorganisms in the sick hippocampus and the healthy hippocampus are compared through a high-throughput sequencing technology, and targeted separation, identification and drug sensitivity screening are further carried out by taking statistically significant difference bacteria as the guidance of potential pathogenic bacteria.

The invention discloses a preparation method of a dairy cow placenta extract CP2 and application of the dairy cow placenta extract CP2 as an antioxidant substance. The preparation method comprises the following steps: by taking normal delivery healthy Chinese Holstein dairy cow placenta homogenate as a substrate, homogenizing by using a tissue homogenizer, adding papain, acting for 4.7 hours under the conditions that the pH is 6.5 and the temperature is 55 DEG C, centrifuging after inactivating for 10 minutes in a boiling water bath, and collecting supernatant, thereby obtaining the dairy cow placenta extract CP2. The preparation method comprises the following steps: performing freeze drying to obtain CPE, separating CP2 in the CPE through chromatography, the amino acid composition of the CP2 is QPGLPGPAG (H-Gln-Pro-Gly-Leu-Pro-Gly-Pro-Ala-Gly-OH), the molecular formula of the CP2 is C35H56N10O11, the molecular weight of the CP2 is 792.89 Da, and the CP2 has good free radical scavenging ability, lipid peroxidation inhibition ability, transition metal chelating ability and reducing power, and can activate a Keap1 / Nrf2 pathway in oxidative stress cells so as to relieve the oxidative stress of the cells.

This utility model discloses a biological tissue homogenizer, specifically relating to the field of cell tissue homogenization technology. It includes a homogenization mechanism comprising a base and a support frame. A placement mechanism is slidably connected above the support frame, and a hydraulic cylinder is mounted above the support frame. This utility model achieves automatic cleaning and surface moisture adsorption of the homogenizing rod through the coordinated operation of the placement mechanism, cleaning mechanism, wiping mechanism, connecting rope, and water tank. The placement mechanism and cleaning mechanism are linked by guide wheels and slide rails, allowing for simultaneous placement and removal of the homogenizing tube, reset of the cleaning mechanism, and preparation for cleaning the homogenizing rod with a single pull, simplifying the operation process. The wiping components (door panel, absorbent cotton) employ an automatic reset design with torsion springs to ensure unobstructed movement of the homogenizing rod, and the absorbent cotton is periodically squeezed by a squeezing block to expel moisture, extending its service life.

The invention belongs to the field of animal models, particularly relates to a granulomatous mastitis animal model and a construction method thereof, and aims to solve the problems of low pathological matching degree and poor repeatability of an existing model. The construction method comprises the following steps: selecting an SPF-grade BALB / c female mouse (10 weeks old, 20-22 g), preparing human mammary gland fresh lesion tissue homogenate and lesion origin staphylococcus aureus liquid (1.4 * 10 < 8 >-1.5 * 10 < 8 > CFU / mL), mixing and emulsifying with a Freund's incomplete adjuvant according to a ratio of 1: 1: 1, injecting to the third pair of mammary glands of the mouse, and completing modeling in two weeks. The pathological characteristics of the model are highly matched with human diseases, the success rate is high, the repeatability is strong, and the model can be used for GM pathogenesis research and therapeutic drug screening.

The utility model provides a food tissue homogenizer, and relates to the technical field of food tissue homogenizers, the food tissue homogenizer comprises a homogenizer base, a mounting frame and a homogenizing cup, through the arrangement of a cleaning device, when cleaning and maintenance are needed, a limiting rod can be aligned with a rectangular groove, and the homogenizing cup is arranged in the rectangular groove; meanwhile, one end of the mounting ring block is aligned with the inner wall of the mounting frame to clamp the mounting ring block and the mounting frame at the same time, then the spiral and the homogenizing cup are fixed, and similarly, the crushing cutter is driven to rotate in the same way as the crushing and homogenizing treatment process; the first brushes and the second brushes can be driven to rotate in the inner wall of the mounting frame and the inner wall of the homogenizer to clean the inner walls of the mounting frame and the homogenizer, the two second brushes are arranged on the two sides of the crushing cutter, dead corners between blades of the crushing cutter and the mounting frame can be cleaned, and therefore the hands of cleaning personnel can be prevented from being accidentally injured, and the cleaning efficiency is improved. The problem of potential safety hazards during cleaning and maintenance is reduced, the homogenizer is convenient to clean, and the use efficiency of the homogenizer is improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a kit for breast cancer diagnosis and application thereof. The kit comprises an elisa plate coated with a captured antibody and an HRP labeled antibody working solution, the capture antibody is a monoclonal antibody Y2A-1; the HRP labeled antibody is a monoclonal antibody P4X-2; the heavy chain amino acid sequence of the monoclonal antibody Y2A-1 is as shown in SEQ ID NO.1, and the light chain amino acid sequence of the monoclonal antibody Y2A-1 is as shown in SEQ ID NO.2; the heavy chain amino acid sequence of the monoclonal antibody P4X-2 is as shown in SEQ ID NO.3, and the light chain amino acid sequence of the monoclonal antibody P4X-2 is as shown in SEQ ID NO.4. The kit for breast cancer diagnosis can quantitatively detect the PPIP5K2 protein content in a human tissue homogenate sample, serves as a breast cancer auxiliary diagnosis means for highly expressing PPIP5K2 proteins, and has a wide application prospect.

This utility model discloses a tissue filtration device, including a centrifuge tube, a first filter element, a second filter element, and a propulsion component. The centrifuge tube has an inner cavity with an opening. The first filter element is installed in the inner cavity and has a first filter chamber. The first filter chamber has a first filter hole and an opening, which are connected to the first filter chamber. Tissue homogenate can pass through the first filter hole, while connective tissue is blocked by the first filter element and remains in the first filter chamber. The second filter element is installed at the bottom of the first filter chamber and has a second filter chamber. The second filter chamber has a second filter hole and an opening, the diameter of which is smaller than that of the first filter hole. The second filter hole and the opening are connected to the second filter chamber, where connective tissue is collected. The propulsion component is located in the first filter chamber and is used to propel the tissue homogenate towards the second filter element. The propulsion component applies pressure to the tissue homogenate. This utility model can reduce the residual rate of connective tissue in tissue homogenate, shorten processing time, and improve filtration efficiency.

The invention discloses a mounting rack for a biological tissue homogenizer, and relates to the technical field of biological tissue homogenization, and the mounting rack is characterized by comprising an adjusting rod mounted on the homogenizer, a clamping mechanism is mounted on the adjusting rod, and the clamping mechanism is arranged right below a homogenization head; the clamping mechanism comprises a torsion ring, a limiting ring A, a clamping ring and a limiting ring B, and the torsion ring, the limiting ring A, the clamping ring and the limiting ring B are sequentially arranged, so that the clamping mechanism can quickly clamp the homogenate cup, and under the driving of the torsion ring, the abutting blocks always move outwards at equal intervals from a circle center, so that the homogenate cup can be quickly clamped. When the homogenate cup is clamped and fixed, the abutting block pushes the homogenate cup towards the center, so that the center position of the homogenate cup can be always aligned with the homogenate head, the homogenate cup can be conveniently fixed, the clamping steps are simplified, and the clamping efficiency during continuous clamping is improved.

The present invention discloses a device for testing the fat content in aquatic organisms, which includes a tissue homogenization and reagent delivery system, a filtration and extraction system, a heating and reagent recovery system, a weighing system, and an automatic control and display system, as well as a method for testing the fat content in aquatic organisms by using this device. The present invention changes the previous high costs of instrument equipment and the previous time-consuming and cumbersome fat extraction methods, providing technical support for determining the fat content in aquatic organisms.

This invention discloses an in-situ vitrification-semi-solid nucleic acid room-temperature stable preservation solution, its preservation method, and a reagent kit, belonging to the field of biological sample preservation technology. The preservation solution comprises a double buffer system, a mild chelation system, a vitrification framework, a thermoresponsive gelling system, an oxidative protection system, a low-toxicity antibacterial system, and water. The thermoresponsive gelling system forms a weakly reversible gel network within the range of 18-37°C after the sample is mixed with the preservation solution, placing the sample nucleic acid in a confined microenvironment with low diffusion and low water activity. This invention achieves long-term stable preservation of nucleic acid samples at room temperature through the synergistic effect of a triple mechanism of "low water activity - thermoresponsive gelling - multi-target passivation." This invention eliminates the need for cold chain transportation, is simple to operate, and has high safety, making it suitable for the room-temperature collection, transportation, and preservation of various biological samples such as blood, saliva, oral swabs, sputum, and tissue homogenates.

The application discloses a tissue homogenizer for detecting bulb vegetables, and relates to the technical field of food detection. The top end of the machine base is fixedly connected with a limiting ring, the inner side of the limiting ring is provided with a lower cylinder, the top end of the lower cylinder is spirally connected with an upper cylinder, the top end of the upper cylinder is spirally connected with an end cover, the inner side of the end cover is rotatably connected with a connecting shaft, the outer side of the connecting shaft is provided with a source suppression mechanism and a defoaming mechanism, the source suppression mechanism is arranged, the surface layer slurry (the region close to the liquid surface) is forced to be pressed to the bottom of the cavity, the design avoids the problems that the traditional cutter swings outward to throw the slurry and impacts the liquid surface, and cuts off the path that the air is rolled into the slurry from the source; the slurry smoothly flows downward along the inclined surface of the cutter, and there is no rolling air phenomenon, so that the air bubbles generated due to the turbulent flow are eliminated from the source.

The invention provides a method for detecting nano-plastics in an organism. The method comprises the following steps: exposing a to-be-detected organism by taking pre-prepared nano-plastics loaded with metal elements as an exposure material; performing homogenization treatment on each tissue of the exposed organism to be detected to obtain tissue homogenate of each tissue; respectively adding an alkaline digestion agent into the tissue homogenate of each tissue; and respectively measuring the digested tissue homogenate of each tissue in a single-particle mode of an inductively coupled plasma mass spectrometer to obtain quantitative and traced detection results of the in-vivo nano plastic of the living body to be detected. According to the method, the to-be-detected organism is exposed by using the metal-loaded nano-plastic, then the tissues of the organism are analyzed one by one, the detection quantitative results of the nano-plastic in the tissues are obtained, the migration process of the nano-plastic in the organism can be deduced according to the physiological relationship between the tissues, and the tracing detection result is obtained.

The invention is applicable to the field of food processing, and provides a preparation method of pig spleen oligopeptide capable of improving immunity, which comprises the following steps: step 1, selecting qualified pig spleen after inspection and quarantine, cleaning, adding a proper amount of clear water for homogenization treatment, then heating to 90-95 DEG C, and keeping the temperature for 15 minutes; step 2, cooling the tissue homogenate after enzyme deactivation to a proper temperature, and carrying out enzymolysis treatment; step 3, adjusting the pH value and temperature of the enzymolysis system to optimal reaction conditions, and continuing enzymolysis; 4, after enzymolysis is completed, standing and precipitating, and separating supernatant enzymatic hydrolysate from residual precipitates; and step 5, performing concentration, dialysis and drying treatment on the enzymatic hydrolysate obtained by separation to finally obtain the pig spleen oligopeptide. The method is simple and convenient to operate, low in cost and high in raw material utilization rate, and the obtained product has the multiple advantages of high purity, easiness in absorption, rich nutrition, strong immunoregulation function and the like.

The invention relates to a construction method of a damp-heat downward flow type erectile dysfunction animal model, which comprises the following steps: S1, from the day of modeling, feeding mice with high-sugar and high-fat feed, and simultaneously providing mixed drinking water containing alcohol and honey, free diet and water; s2, from the eighth day of modeling, feeding the mice in a humid and hot environment; s3, on the 15th day, the 20th day and the 25th day of modeling, a prostate tissue emulsion is adopted to conduct subcutaneous injection on the mouse, a cyclophosphamide solution is synchronously injected into the intraperitoneal cavity, and the prostate tissue emulsion is obtained by emulsifying prostate tissue homogenate and a complete Freund's adjuvant according to the volume ratio of 1: 1; and S4, carrying out comprehensive evaluation on the damp-heat downward flow type erectile dysfunction animal model. Internal and external damp-heat factors are combined with drug induction, the pathogenesis of'damp-heat accumulation in lower jiao 'in traditional Chinese medicine is cooperatively simulated through multiple mechanisms, the manifestation symptom of the animal model is almost consistent with the disease symptom of a clinical damp-heat downward flow type impotence patient, and good scientificity and reliability are achieved.

The utility model relates to an animal tissue homogenizer, and belongs to the technical field of sample preparation. The animal tissue homogenizer comprises a sliding rod 100, a sleeve 300 arranged on the sliding rod 100 in a sleeving mode and a homogenizing cage 200 arranged at one end of the sliding rod 100, and a pull ring 110 is arranged at the other end of the sliding rod 100. The homogenate cage 200 is made of an elastically deformable material, and the pull ring 110 is used for pushing and pulling the sliding rod 100 to slide in the sleeve 300, so that the homogenate cage 200 is folded in the sleeve 300, or the homogenate cage 200 recovers an expansion state after extending out of the sleeve 300. By arranging the homogenizing cage which can move up and down and contract, not only is the stirring function reserved, but also the homogenizing cage can move up and down and contract and expand, so that operations such as cutting and stirring can be performed on animal tissues, and the homogenizing efficiency and the homogenizing effect of the animal tissues are improved.

The invention discloses a preparation method and application of cyclina sinensis oligosaccharide, belongs to the technical field of deep processing of shellfish aquatic products, and particularly relates to a technology which comprises the following steps: cleaning new cyclina sinensis meat for three times, mincing, soaking with 75% ethanol, crushing and freeze-drying with a tissue homogenizer, adding an acetone solution according to a material ratio of 1: 4 (w / v), degreasing for 2 hours, and filtering to obtain the cyclina sinensis oligosaccharide. Performing ultrasonic treatment for 30 minutes, performing enzymolysis for 8 hours by using papain, adding 4 times of absolute ethyl alcohol in volume, standing for 24 hours in a chromatography cabinet at 4 DEG C, repeatedly performing alcohol precipitation for 2-3 times, and performing freeze-drying to obtain a cyclina sinensis polysaccharide crude product; cyclina sinensis polysaccharide crude products are purified through an anion exchange column and a gel permeation chromatographic column, then the cyclina sinensis oligosaccharide is prepared through an acid hydrolysis method, the prepared cyclina sinensis oligosaccharide has good antioxidant activity, when the concentration is 5.0 mg / mL, the scavenging rates of DPPH, hydroxyl free radicals and superoxide anions are 89.93%, 70.55% and 83.66% respectively, and the total antioxidant capacity is 23.15 U / mg by mass. The compound has remarkable cytotoxic activity on human lung cancer cells A549, and the IC50 value of the compound is 1.13 mg / mL. The invention provides a new thought and reference for deep processing and high value of the cyclina sinensis.

The utility model discloses a handheld tissue homogenizer which comprises a homogenizer shell, a cavity is formed in the middle of the homogenizer shell, the cavity is divided into a battery bin, an elastic contact is installed at the bottom of the battery bin, a rechargeable battery is installed in the battery bin, the inner wall of the cavity is fixedly connected with a supporting plate, and the supporting plate is fixedly connected with the inner wall of the cavity. The upper end of the supporting plate is fixedly connected with a motor, and the lower end of the supporting plate is rotationally connected with a driven gear and a driving gear. A transmission shaft is fixedly connected to the middle of the driven gear, a connector is fixedly connected to the lower end of the transmission shaft, a homogenizer is installed in the middle of the lower end of the connector, and a fixing mechanism is arranged at the lower end of the connector. The homogenizer can be provided with a power supply and is convenient to carry and use, and meanwhile, a test tube in which tissues are stored can be connected and fixed with the homogenizer, so that the homogenizer can be directly held by hand for use and is convenient to operate.