6827 results about "Physiology" patented technology

Compositions and methods for editing deleterious mutations associated with Alpha-1 Antitrypsin Deficiency (A1AD). In particular embodiments, the invention provides methods for treating A1AD using a modified adenosine base editor with improved on-target editing and decreased off-target editing to correct mutations associated with A1AD.

A sheep single nucleotide polymorphism (SNP) molecular marker and use thereof in detecting resistance to brucellosis in sheep are provided, relating to the technical field of molecular marker-assisted breeding. A sheep SNP molecular marker is provided, where the sheep SNP molecular marker is associated with resistance to brucellosis in sheep, and has a nucleotide sequence shown in SEQ ID NO: 1 in which a T / C polymorphism is located at 101st bp. The sheep SNP molecular marker can accurately detect a resistance level of sheep to the brucellosis, and is used for the selection of brucellosis-resistant sheep as well as molecular marker-assisted breeding of the resistance to brucellosis in sheep. The sheep SNP molecular marker effectively improves a breeding efficiency of sheep with brucellosis resistance.

Systems and methods for monitoring glucose levels of a user are described. Data indicative of glucose levels of the subject is received. A count is assigned for each glucose episode based at least on an area under the curve of the each glucose episode in a dataset of time-correlated glucose data. A running sum of counts for each glucose episode in a time period is calculated. The running sum of the counts may be displayed in a numerator of a fraction and a target count goal in a denominator of the fraction. A progress indicator representative of the running sum of the counts relative to a target count goal for the time period may also be displayed. A length of a variable portion may be proportional to the running sum of the counts, and a total length of the progress indicator may be proportional to the target count goal.

Disclosed herein are “ketone body-HMB” or “KB-HMB” compositions including a combination of: (1) hydroxymethyl butyrate; (2) a ketone body component such as beta-hydroxybutyrate (BHB) and / or acetoacetate; and (3) optionally a dietetically or pharmaceutically acceptable carrier. Also disclosed herein are methods of using such compositions for producing desired physiological effects, such as fat loss, in a mammal. The compositions beneficially enhance fat loss through ketosis while also reducing or eliminating muscle wasting and / or promoting muscle formation.

The invention relates to the field of preparation and application of biological peptides, in particular to a hippocampal swelling peptide and application thereof in preparation of functional products for repairing male reproductive system injury. A green controllable enzymolysis technology is utilized to obtain protein polypeptide with nutritional, health-care and medicinal values, five active peptide sequences with high biological activity are obtained through further research on the basis, the high-purity active peptide is obtained through a chemical synthesis mode, and the biological activity of the peptide is verified through mouse spermatogonium.

1. The name of the product of this design: Breast Pump (S07). 2. Purpose of the product of this design: to assist lactating women in extracting breast milk. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention provides infant formula milk powder for improving intestinal inflammation and / or microbial disorder as well as a preparation method and application of the infant formula milk powder. The invention provides infant formula milk powder for the first time, the infant formula milk powder comprises 2 '-fucosyllactose and lactose-N-neotetraose, and the mass ratio of the 2'-fucosyllactose to the lactose-N-neotetraose is (1-5.5): 1. The infant formula milk powder can improve intestinal inflammation and / or microbial disorder.

The invention relates to the technical field of cytology, in particular to a grass carp brain astrocyte line and application thereof, the grass carp brain astrocyte line is preserved in China Center for Type Culture Collection on May 7, 2025, and the preservation number of the grass carp brain astrocyte line is CCTCC NO: C2025149. The grass carp brain astroglia cell line provided by the invention has the capability of efficiently proliferating GCRV-II, and the virus titer of the GCRV-II replicated in the cell line at least can reach 1.38 * 10 < 8 > pfu / mL or above; after the GCRV-II is blindly passed for 5 generations in a grass carp astroglia cell line, the grass carp can still have typical bleeding symptoms and death due to the virus. And the exogenous plasmid transfected grass carp brain astroglia cell line has similar transfection efficiency to commercial grass carp kidney cells. Therefore, the invention lays an important foundation for deep research of pathogenic mechanism of GCRV-II, vaccine preparation, antiviral drug screening and prevention and control of grass carp viral hemorrhagic disease.

The invention provides application of a protein deacetylase inhibitor NAM in ultralow-temperature cryopreservation of poultry semen, and belongs to the technical field of poultry breed conservation. The protein deacetylase inhibitor NAM is used as a cryoprotectant or is combined with other cryoprotectants for use, and the protein deacetylase inhibitor NAM is used as an effective component, so that the ultralow-temperature cryopreservation efficiency of poultry can be improved. Wherein the protein deacetylase inhibitor NAM can inhibit sperm cell deacetylation modification so as to maintain a high acetylation modification level, improve the ability of sperms to respond to ultralow temperature freezing stimulation and improve the fertilization ability of frozen-remelted sperms.

The invention relates to the technical field of grass carp, in particular to a molecular marker associated with body fat traits of grass carp and application. The invention discloses SNPs molecular markers associated with a grass carp FABP3 gene and a body fat character. The SNPs molecular markers are respectively located at the basic group positions of 29945677, 29948389 and 29948502 of a No.19 chromosome of a grass carp reference genome GCF019924925.1. The three SNPs molecular markers are closely associated with body fat traits such as the abdominal fat index, the liver fat content and the muscle fat content of the grass carp. The molecular marker can be applied to molecular-assisted selective breeding of low-fat grass carp strains.

The invention relates to application of fecal microbe transplantation (FMT) in depressive disorder treatment, in particular to application of a fecal microbe transplantation (FMT) composition based on a human intestinal tract fecal microbe composition in depressive disorder treatment. The invention also relates to application of the pharmaceutical composition or the medicine box in preparation of a medicine for treating depressive disorder, and the pharmaceutical composition or the medicine box contains a therapeutically effective amount of the human intestinal tract coprophilous fungus composition.

The invention relates to the field of biological detection, in particular to the field of paternity test and detection, and particularly relates to a method for non-invasive prenatal genetic relationship judgment by adopting a two-state SNP (Single Nucleotide Polymorphism) site. According to the method, the minimum number of SNP sites required for judging the genetic relationship is calculated, the genetic relationship is judged by screening the sites containing fetal information in the free DNA sample of the pregnant woman and combining the indexes, the genotype of the pregnant woman sample does not need to be additionally detected, meanwhile, whether the parent-child relationship between a fetus and a suspected parent conforms to the parent-child relationship or not can be accurately judged, and the application range is wider.

The invention belongs to the technical field of microorganisms, and relates to high-activity plant lactobacillus KL100 for relieving anxiety and depression and application of the high-activity plant lactobacillus KL100. The plant lactobacillus provided by the invention is KL100, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has a preservation number of CGMCC No.27192, and is classified and named as the plant lactobacillus. The plant lactobacillus is KL100, has a good gastrointestinal passing rate, shows a breeding characteristic in the stomach after being embedded, is improved in cholate-resistant survival rate, and can be used for preparing the plant lactobacillus. According to the application disclosed by the invention, the expression of IL-1beta and TNF-alpha in a mouse body can be effectively reduced, the expression of IL-10 can be improved, and the secretion of 5-hydroxytryptamine by chromaffin cells in intestinal tracts can be promoted, so that anxiety and depression can be relieved, the strain resource is expanded, the development of the biological medicine field is promoted, and a new direction is provided for treating anxiety and depression.

The invention discloses an HNRNPA2B1 gene-based bovine sperm malformation rate related SNP molecular marker and application thereof, and belongs to the technical field of genetic molecular biology, the number of the SNP molecular markers is two, SNP1 is at the position of a bovine genome chr4: 69733358, the polymorphism is C or A, SNP2 is at the position of a bovine genome chr4: 69733387, and the polymorphism is T or G. According to the two SNP loci provided by the invention, the dominant genotypes of g.-1713A > C and g.-1684G > T are AA and GG respectively, and the sperm collection quantity and the sperm malformation rate of the two SNP loci have better performance. By detecting the polymorphism of the two SNP loci, the motility evaluation of the bull sperms can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention provides an SNP (Single Nucleotide Polymorphism) site of an ABO blood group variant, and an A subtype variant blood group identification kit and method, and relates to the technical field of biology. The SNP sites of the ABO blood group variant comprise the 155th c.155Ggt from an initiation codon of an ABO gene coding region, and the 155th c.155Ggt from the initiation codon of the ABO gene coding region; mutation of T. A substance for detecting the SNP site of the ABO blood group variant can be applied to preparation of an A-subtype variant blood group identification method and product so as to be effectively applied to rapid detection of patients and blood donors, the A-subtype variant blood group of an individual can be defined, and possible blood transfusion reaction can be prevented in time.

The invention belongs to the field of biological medicines, and particularly relates to application of FeTMPyP in preparation of a medicine for relieving ovarian toxicity. FeTMPyP is selected as an intervention agent aiming at the fact that MC-LR and NaNON combined exposure can remarkably induce mouse ovary nitration stress, mouse follicle barrier damage and ovarian reproductive endocrine dysfunction; the FeTMPyP can be used for effectively relieving nitration stress induced by combined exposure of MC-LR and NaNO2 in mouse ovaries, improving sex hormone disorder, disordered estrus cycle and follicular atresia phenomena caused by combined exposure of the two poisons, and recovering the fertility of the mouse, so that the intervention of the FeTMPyP can be used for relieving the mouse ovarian toxicity induced by combined exposure of MC-LR and NaNO2, and can be used for treating the ovarian toxicity of the mouse caused by combined exposure of the MC-LR and NaNO2. The compound can be used for preparing medicines for preventing and treating related diseases such as mouse ovarian toxicity caused by environmental toxin exposure, and a new way is provided for early recognition and targeted intervention of ovarian related diseases.

The invention relates to a metabolic marker for diagnosing diabetic secondary osteoporosis and application of the metabolic marker, and belongs to the technical field of biological medicine. The method comprises the following steps: firstly, screening out obviously different metabolites between osteoporosis (DOP) and osteoporosis-free groups (DM) in diabetic patients; and secondly, screening out significant difference metabolites between the primary osteoporosis patient and the healthy control group, and excluding the intersection of the two groups of difference metabolites to obtain metabolites which exclude the influence of the primary osteoporosis. Metabolic markers, namely cytosine nucleotide, ketoglutaric acid and triethylamine, are obtained through further screening, and when the three metabolic markers are independently used, AUCgt; the AUC during combined use is 0.99, so that the limitation that the sensitivity of the traditional bone mineral density detection on the osteoporosis recognition in the diabetic population is insufficient is effectively made up.

The invention discloses an application of an HERP gene knockout mouse model in obesity and metabolic disorder diseases. The invention finds that compared with a wild type high-fat group, HERP gene knockout can cause phenotypes such as early obesity, more serious obesity degree, increase of fat weight ratio, aggravation of adipose tissue inflammation, dyslipidemia and glucose metabolism disorder of mice during high-fat feeding. And the HERP gene is knocked out, so that the adipocyte differentiation is increased. The HERP gene knockout mouse is more sensitive to nutrition, obesity can occur more quickly during high-fat feeding, symptoms such as fat amplification, fat inflammation and glucose and lipid metabolism disorder are more serious, and the HERP gene knockout mouse can be used for molecular mechanism research and drug screening of obesity, fat tissue and glucose and lipid metabolism disorder. It is further found that cilostazol can significantly relieve mouse obesity and lipid metabolism disorder caused by HERP gene defects, but has no obvious weight losing effect on normal genotype mice, and a new strategy is provided for precise treatment.

The invention discloses a multi-target fusion dairy cow oestrus behavior detection method and system, and belongs to the technical field of target detection of deep learning. The method comprises the following steps: S1, collecting a dairy cow oestrus behavior image, and performing image enhancement on the dairy cow oestrus behavior image; s2, constructing a PBF-YOLO deep learning network model, wherein the PBF-YOLO deep learning network model comprises a lightweight module, a background information enhancement module and a feature aggregation module; and S3, identifying a dairy cow oestrus behavior region of interest through the PBF-YOLO deep learning network model, and detecting the dairy cow oestrus behavior. The invention provides a solution for the situation that the existing lightweight algorithm is low in detection precision and poor in robustness due to the fact that the cow oestrus behavior and other background features are similar and are difficult to distinguish in an actual pasture and cows with different scales appear on the same image.

The invention discloses lactobacillus reuteri CCFM1288 for relieving depressive mood and application of the lactobacillus reuteri CCFM1288, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1288 has the tryptophan metabolism capability, and can survive for 7 days in the intestinal tract; after the lactobacillus reuteri CCFM1288 intervenes a depressive mouse, anxiety-like and depression-like behaviors of the mouse can be obviously relieved, flora alpha-diversity is improved, intestinal symbiotic bacteria are promoted to secrete indole substances, intestinal homeostasis is maintained, hypothalamic-pituitary-adrenal axis hyperfunction is relieved, dopamine and serotonin neurotransmitter nervous system functions are improved, and the effect of preventing and treating depression is achieved. The neurotransmitter release is promoted, and the anti-depression effect is achieved. In clinical tests, intervention of Lactobacillus reuteri CCFM1288 can significantly improve the depressive symptom of MDD patients, and a new safe alleviation approach is provided for depression treatment.

The invention discloses a method for evaluating postprandial blood glucose response of a human body based on continuous blood glucose data and application. The method comprises the following steps: acquiring a continuous blood glucose detection value and meal starting time; matching a first blood glucose recording point after eating according to the dining time, intercepting blood glucose data of 2 hours after meal, and preprocessing the data; constructing four postprandial blood glucose response modes by applying the continuous blood glucose data of FPCA and based on a characteristic function of which the interpretation degree ranks top two obtained by the FPCA; through comparison of postprandial blood glucose response mean value curves of the four blood glucose response modes, the four response modes can evaluate postprandial blood glucose by integrating the blood glucose concentration range and whether the fasting homeostasis can be rapidly recovered or not.

The invention provides a male product delay spray and a preparation process thereof, and relates to the field of delay sprays. The preparation process of the male product delayed spray comprises the following steps: S1, preparing water and ethanol, and uniformly stirring and mixing; s2, extracting fructus cnidii, herba epimedii, flos caryophylli, pericarpium zanthoxyli, herba cistanche, herba cynomorii and piper nigrum respectively; s3, obtaining a corresponding extract according to the step S2; s4, putting the obtained extract into water and an ethanol dissolving solution; s5, increasing the stirring speed, heating, and fully dissolving and mixing; s6, stopping stirring and cooling to room temperature; and S7, performing filling. According to the invention, the plant extract is adopted as an effective component, and the special pepper kava extract is selected, so that the irritation can be obviously reduced, the delay effect can be obviously improved, and the effects of reducing the skin sensitivity of the penis and improving the sexual intercourse time can be achieved by applying the plant extract to the skin surface of the male penis.

The present invention provides a combination comprising lactose and human milk oligosaccharides (HMOs) 2'-fucosyllactose (2'-FL), lacto- / V-neotetraose (LNnT), 3- fucosyllactose (3'-FL), at least one of 3'-sialyllactose (3'-SL) or 6'-sialyllactose (6'-SL), and optionally lacto-N-tetraose (LNT) for use in the dietary management of dysbiosis of the gut microbiota in a subject or for use in the dietary management of a disease or disorder associated with metabolism and / or accumulation of BCFA in a subject.

A method may include obtaining blood glucose level readings over a diurnal period for each of a plurality of days and determining an estimated variability of the blood glucose levels over the diurnal period for the plurality of days. The method may also include modifying, based on the estimated variability of the blood glucose level, a target blood glucose level to a modified target blood glucose level, and delivering insulin, using an insulin pump, during the diurnal period based on the modified target blood glucose level.

The invention belongs to the technical field of biomolecules, and particularly relates to an NIPBL gene for judging the sex of chickens and application of the NIPBL gene. The NIPBL gene has a segment with the difference of 606bp on chicken Z and W chromosomes, and is also a deletion segment on the W chromosome, the chicken sex can be quickly and effectively identified by detecting the sequence condition, the chicken with the deletion of the molecular marker sequence segment is a female individual, and the chicken without the deletion of the molecular marker sequence segment is a male individual; the invention further provides a specific primer, the chicken sex can be accurately judged through a PCR amplification technology and agarose gel electrophoresis, a strip is clear, the method is small in technical difficulty, easy and convenient to operate and high in accuracy, the sex of chicks or chickens difficult to distinguish in appearance can be effectively identified, the method has practical significance on identification of varieties small in male and female appearance difference, and the method is worthy of popularization and application. The method disclosed by the invention is strong in technical applicability and good in repeatability, can be used for accurately identifying the gender of the poultry, and has a wide application prospect.

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

A molecular marker related to body weight traits of Hu sheep and an application are provided. The whole genome sequencing of Hu sheep is carried out in the invention, the combination of QTL and GWAS is adopted, the candidate genes related to the weight traits are analyzed and the three molecular markers of MAP3K1, ABCB1, and MEF2C are selected as candidate genes for the weight traits of Hu sheep, and the two molecular markers of ANKRD55 and TRNAW-CCA-87 can be used as potential candidate genes for the weight traits of Hu sheep, these candidate genes can be used as molecular markers for application, the guidance of Hu sheep breeding will help to understand the genetic mechanism of weight traits of Hu sheep and further guide Hu sheep breeding.

The invention belongs to the technical field of biological medicines, and discloses application of urolithin A in preparation of medicines or health-care foods for delaying ovarian aging. The invention proves that by supplementing urolithin A, the fertility of the senescent ovary can be improved, the estrus cycle of the senescent ovary can be shortened, the fibrosis level of a senescent ovary matrix can be reduced, the ovary reserve capacity can be improved, the quality of a senescent ovary oocyte can be improved, and the fertilization and embryonic development potential can be improved; it is proved that urolithin A can be used as an effective component for preparing the medicine or health food for delaying ovarian aging.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention relates to an application of a regulator in improving the maturation quality of frozen pig oocytes, the regulator is N-(2-(2-(4-chlorphenyl) thiazole-4-yl) ethyl) butyryl, and the regulator improves the maturation quality of the frozen pig oocytes by improving the mitochondrial function of the frozen pig oocytes after in-vitro maturation. The invention provides a new application of N-(2-(2-(4-chlorphenyl) thiazole-4-yl) ethyl) butyramide, namely the N-(2-(2-(4-chlorphenyl) thiazole-4-yl) ethyl) butyramide is used for developing a novel oocyte vitrification freezing solution and an in-vitro maturation culture solution, and the obtained high-quality oocytes can be beneficial to embryo biotechnology researches such as in-vitro fertilization and somatic cell nuclear transplantation.