29 results about "Embryo transplantation" patented technology

Disclosed are methods for producing controlled endometrial organoids using micropatterning techniques, and methods for using endometrial cells or organoids for diagnostic and predictive purposes, and for optimizing IVF and other infertility-related treatments, including embryo transfer and intrauterine insemination (IUI). These organoids grow in a spatially controlled manner and exhibit less heterogeneity than organoids grown directly in a three-dimensional culture. The specification also describes methods for predicting the success of embryo transfer or other IVF-related treatments, optimizing embryo transfer, improving embryo implantation in the endometrium, and performing embryo transfer in subjects. These methods include culturing endometrial organoids or endometrial epithelial cells, and contacting the endometrial organoids or cells with one or more blast-like cells. In some embodiments, the endometrial organoids or cells include endometrial cells obtained from subjects undergoing or considering IVF-related treatment.

The invention belongs to the technical field of GPCR drug research and development, and discloses a ratio type cAMP fluorescent probe RaCamp and a construction method of a transgenic mouse, the probe RaCamp is formed by series fusion of G-Flamp2 fluorescent protein and mCherry fluorescent protein through a flexible linker, and the preparation method of the probe RaCamp comprises the following steps: S1, obtaining an mCherry fragment; s2, a G-Flamp2 linear carrier is obtained; s3, homologous recombination; s4, converting and screening; the construction method of the transgenic mouse comprises the following steps: T1, designing a carrier; t2, microinjection of fertilized eggs; t3, embryo transplantation and reproduction; the invention has the beneficial effects that mCherry is added as an internal reference protein on the premise of keeping the sensitivity of the original G-Flamp2 probe, and the selected connecting peptide (GGGGS) 2 enables the expression quantity of the two proteins in cells to be accurately controlled to be 1: 1, so that the problem of background interference in cell observation of in-vivo imaging is well solved. And the sequence is inserted into a Rosa26-LSL box, so that the construction of a Rosa26-LSL-RaCamp mouse is realized.

The application provides a construction method of a humanized keloid mouse model, comprising the following steps: BAC plasmid construction and preparation; superovulation of experimental mice and collection of zygotes; pronuclear microinjection of zygotes; post-injection embryo transplantation; genotype identification; breeding and genetic analysis; and verification of construction results of the humanized immune system by immunohistochemistry and immunofluorescence. In the application, peripheral blood mononuclear cells treated by sCD27 and skin around keloids (homologous cells and tissues) are transplanted into NSG-MHC-DKO immune-deficient mice with overexpression of CD70 genes, so that HLA rejection of different homologous immune cells and tissues can be avoided, the internal microenvironment of keloids and the interaction between the internal microenvironment and the immune system can be restored to the maximum extent, and the influence of immune factors on the occurrence and development of keloids can be realized in vitro. The application first discovers and verifies that the activation of the CD27-CD70 axis can promote the occurrence and development of keloids, and provides a suitable animal model for the research and development of anti-keloid drugs, especially immunotherapy.

The invention discloses a construction method of a Klf6 gene knockout mouse embryo model, which comprises the following steps: S1, preparing a gene editing solution containing Cas9 enzyme and sgRNA for targeting a third exon region and a fourth exon region of a mouse Klf6 gene by utilizing a CRISPR / Cas9 gene editing technology; s2, taking out the fertilized eggs of the mouse, transfecting the fertilized eggs of the mouse with a gene editing solution, and then performing in-vitro culture on the fertilized eggs; s3, carrying out embryo transplantation on the two-cell stage embryo obtained in the step S2 into a pregnant female mouse, and then carrying out conventional feeding; and S4, 7-8 days after embryo transplantation, taking out the uterus and stripping the embryo to obtain the Klf6 gene knockout mouse embryo model. According to the method, gene knockout is carried out by using CRISPR / Cas9 in the fertilized egg period in combination with an electrotransfection method, and compared with an existing method, the construction process time can be greatly shortened, the working efficiency is obviously improved, and the success rate is high.

The invention discloses an animal embryo biopsy sampling method and application thereof, and belongs to the technical field of animal breeding. The animal embryo biopsy sampling method provided by the invention is safer, more accurate and more efficient, and can effectively reduce the difficulty of an embryo biopsy sampling technology, reduce mechanical damage to embryos, effectively avoid development retardation of the embryos after sampling and remarkably improve the ratio and utilization efficiency of available embryos on the premise of ensuring the stability of the detection rate of a genetic evaluation chip; the survival rate, the developmental ability and the embryo pregnancy success rate of the sampled embryos are remarkably improved, and the transplantation conception rate of the frozen embryos after sampling reaches 40%; the method can be used for hereditary performance detection and evaluation and embryo screening before embryo implantation, and has important significance in shortening generation intervals, accelerating the breeding process and reducing the overall production cost of animal breeding and breeding.

The invention discloses a repeated implantation failure analysis method based on a multi-modal data fusion model, and the method comprises the steps: collecting basic information of a patient, medical history, laboratory and iconography examination, embryo transplantation and other multi-modal data, integrating dispersed information, providing a comprehensive data basis for analysis, breaking an information island, and enabling the analysis to be based on a complete image; meanwhile, a neural network model is trained according to the preprocessed data to obtain a multi-modal data fusion model, potential correlation among factors can be mined, internal relations can be accurately reflected through fusion features, complex disease causes can be comprehensively revealed, and the limitation that only one aspect can be concerned in the prior art is overcome; besides, a prediction method based on the multi-modal data fusion model is more accurate and reliable than traditional experience judgment and single index analysis, targeted suggestions can be given according to prediction, personalized guidance is provided for clinicians, formulation of precise strategies is assisted, and the defects of precise prediction and personalized suggestions in the prior art are overcome.

The utility model discloses an animal embryo transplantation operating table, and belongs to the technical field of animal embryo transplantation. A groove is formed in the table body, a supporting plate is installed in the groove, four sliding grooves are formed in the top of the table body, the sliding grooves are T-shaped grooves, a fixing assembly comprises a sliding block and a fixing base, a fixing ring is arranged at the bottom of the sliding block, a clamping block is arranged at the bottom of the sliding block, the clamping block is a T-shaped block, the clamping block is clamped in the sliding grooves in a sliding mode, and the fixing base is fixedly connected to the top of the table body. The number of the fixing seats is four, blind holes are formed in the fixing seats, electric push rods are installed in the blind holes, and check blocks are fixedly connected to the moving ends of the electric push rods. According to the operating table, the fixing assembly is arranged, the animal to be operated is fixed on the operating table through the fixing assembly, the situation that the animal struggles in the operation process, and consequently operation is difficult is avoided, and the problem that the animal to be operated cannot be well fixed through the operating table is solved.

This invention relates to a method for constructing a mouse model of autosomal dominant polycystic kidney disease and its applications, particularly to a method for preparing a non-human animal model carrying a nonsense mutation in the Pkd1 gene c.616_618GAG>TAG based on CRISPR / Cas9 gene editing technology. The method involves co-injecting mouse zygotes with gRNA targeting exon 5 of the Pkd1 gene, homologous recombinant donor oligonucleotides containing the c.616_618GAG>TAG mutation, and Cas9 nuclease. Mutant mice are obtained via embryo transfer. The mutation site is verified by PCR combined with sequencing, and a stable mutant line is established through breeding. This invention also includes the application of this model animal as a research tool for autosomal dominant polycystic kidney disease, particularly in the areas of pathogenesis analysis, drug screening, and therapeutic target validation. This model can stably simulate the typical clinical manifestations and pathological features of human autosomal dominant polycystic kidney disease, providing an important tool for basic research and translational medicine of this disease.

The invention provides an endometrial receptivity evaluation method, device and equipment, a medium and a program product. The method comprises the following steps: acquiring a fresh embryo transplantation type, clinical data of a patient and a uterus longitudinal section ultrasonic image of an ovum taking day; performing endometrial segmentation on the ultrasonic image to obtain an endometrial region, and determining a peripheral region based on the endometrial region and a transplantation type; extracting radiomics characteristics of the endometrial region and the peripheral region, and extracting clinical characteristics of the clinical data; and inputting the image omics characteristics and the clinical characteristics into an endometrial receptivity multi-modal evaluation model corresponding to the transplantation type to obtain the pregnancy probability output by the multi-modal evaluation model after the fresh embryo transplantation in the current period. According to the method, the device, the equipment, the medium and the program product provided by the invention, real-time analysis is carried out in combination with an artificial intelligence technology, and an evaluation result of the fresh embryo transplantation period can be provided in a short time.

The utility model discloses a puncture needle, relates to the technical field of embryo transplantation, and aims to solve the problem that embryo transplantation cannot be realized when the genital tract of a patient is abnormal by a transplantation tube in the related technology. The puncture needle is used for embryo transplantation and comprises a needle tube, and the needle tube is a rigid tube; the first end face of one end of the needle tube in the length direction is obliquely arranged relative to the axis of the needle tube to form a needle tip, and the needle tip is used for entering a life body; a first included angle between the first end face and the axis of the needle tube is 15-20 degrees.

This invention provides a method for preparing goats overexpressing the FOXL2 gene, belonging to the field of genetic engineering and transgenic animal technology. The method includes the following steps: transfecting intersex goat embryonic fibroblasts with a vector plasmid overexpressing the FOXL2 gene to obtain a stable monoclonal cell line overexpressing the FOXL2 gene; using the monoclonal cell line as a nuclear donor for nuclear transfer cloning in a recipient goat to obtain cloned goat embryos overexpressing the FOXL2 gene; and transferring the cloned goat embryos into a surrogate goat to produce goats overexpressing the FOXL2 gene. The nucleotide sequence of the FOXL2 gene is shown in SEQ ID NO.1. By overexpressing the FOXL2 gene in intersex goats, the regulatory function of the FOXL2 gene on horn traits is explored, providing insights for reducing or even overcoming the harm of intersex hornless syndrome and obtaining normal hornless dairy goat germplasm.

The invention discloses an adjustable elbow assembly for an embryo transplantation catheter, relates to the technical field of embryo transplantation catheters, and aims to solve the technical problem that an opening of an embryo transplantation outer tube is easy to cause injury to a patient. By means of multi-structure collaborative innovation, the problems that a traditional catheter is high in damage risk, poor in adaptability and the like are solved in an all-around mode, and the catheter has the core advantages that by means of the necking design, the cervical contact area is reduced, a smooth port and a directional deformation structure are matched, scraping and lobe damage are thoroughly avoided, and the complication risk is reduced; the structures of the shaft ring and the traction rope are optimized, the necking state is kept, meanwhile, the flexibility of the pipe body is guaranteed, and smooth pipe insertion is guaranteed; multi-angle adjustment is achieved through the multi-bend arc body and the independent shifting plate, cervical canal differences of different patients are accurately adapted, and the success rate of intubation is increased; the control structure is convenient to operate, and the necking state is stable and controllable. The whole device is designed through mechanical linkage, safety, adaptability and operability are considered, and safety and comfort of an embryo transplantation operation are remarkably improved.

The utility model discloses a transfer cabinet for bovine embryo transplantation, which relates to the technical field of transfer equipment and comprises a transfer cabinet body, and a test tube limiting mechanism and a cooling mechanism are arranged in the transfer cabinet body. The test tube limiting mechanism comprises three test tube limiting seats which are distributed in the transfer cabinet body in parallel, and the three test tube limiting seats are rotationally arranged on the inner bottom wall of the transfer cabinet body. According to the transfer cabinet disclosed by the utility model, the test tube limiting mechanism is arranged in the transfer cabinet, so that embryo test tubes can be limited by utilizing the cylindrical slots in the surfaces of the test tube limiting seats, and the test tubes can be ventilated by utilizing the first strip-shaped vent holes and the second strip-shaped vent holes in the surfaces of the test tube limiting seats and the cylindrical slots; cold air generated by the refrigeration box is conveyed into a cylindrical slot by utilizing a flow guide cavity of a test tube limiting seat, the test tubes are cooled by utilizing the cold air, a low-temperature storage effect is achieved, a uniform low-temperature effect can be kept on the surfaces of the test tubes, and transfer and transportation of embryos are facilitated.

The invention discloses an embryo transplantation booster which comprises a main body module, a quantitative control module, a pushing module, a connection adaptation module, an operation feedback module and a safety protection mechanism, an operation button is arranged at the tail part of the main body module, a coaxial nesting structure of a clutch push rod and a precise screw rod is adopted in the main body module, and a mechanical interlocking mechanism is matched to realize conflict-free switching of double modes of precise rotating speed regulation and uniform pressing labor saving; and the connection adaptive module adopts a Luer cone joint to realize head suction, and an independent adding port is cancelled. The invention further discloses a using method of the embryo transplantation booster, and the using method comprises the steps of segmented precise inhalation in a clean bench, clinical dual-mode pushing and whole-course safety protection, and standardized operation is achieved. The method is high in pushing precision, low in pollution risk, standard in operation process, suitable for diversified embryo transplantation scenes, capable of remarkably improving the embryo survival rate and extremely high in clinical application value.

The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions and methods that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. For example, methods disclosed herein may comprise preparing a Cas9 / RNA complex, wherein the Cas9 / RNA complex comprises a Cas9 protein and a guide RNA. Methods may further comprise introducing the Cas9 / RNA complex into a non-human embryo, wherein the Cas9 / RNA complex induces a modification at a target endogenous nucleic acid of the non-human embryo to provide for a genome modified embryo. Methods may further comprise transferring the genome modified embryo into a foster mother and allowing the foster mother to produce a F0 animal having the modification at the target endogenous nucleic acid.

The invention relates to a construction method and application of an autosomal dominant polycystic kidney disease mouse model, in particular to preparation of a Pkd1 gene carrying c.616618GAGgt gene based on a CRISPR / Cas9 gene editing technology. The invention relates to a method for a non-human animal model of TAG nonsense mutation. The construction method comprises the following steps: co-injecting gRNA (guide Ribonucleic Acid), containing c.616618GAGgt, of a No. 5 exon of a targeted Pkd1 gene into a mouse fertilized egg; carrying out embryo transplantation on TAG mutated homologous recombination donor oligonucleotide and Cas9 nuclease to obtain a mutated mouse; a mutation site is verified by combining PCR (Polymerase Chain Reaction) with sequencing, and then a stably inherited mutation line is established by breeding. The invention also comprises application of the model animal as an autosomal dominant polycystic kidney disease research tool, especially application in the fields of pathogenesis analysis, drug screening and therapeutic target verification. The model can stably simulate typical clinical manifestation and pathological characteristics of human autosomal dominant polycystic kidney disease, and provides an important tool for fundamental research and transformation medicine of the disease.

The invention discloses an embryo transplantation outcome prediction method based on single blastocyst image data, belongs to the field of medical artificial intelligence, and is suitable for single blastocyst transplantation. Firstly, a high-resolution embryo image of a patient is obtained. The image resolution is unified by fixing the aspect ratio of an original image and properly cutting and filling. A standard picture enhancement technology is adopted for model training, and adaptability to variable imaging conditions is improved. During prediction, a preprocessed image is input into a model which takes ResNet-50 as a trunk and combines SimCLR comparative learning to perform self-supervised pre-training, the model is used for extracting morphological features of embryos, and the recognition capability of the model for specific tasks is further optimized through supervised fine tuning. The extracted features are averagely pooled and then connected with a newly defined full-connection network, then pregnancy probability is output through a Sigmoid activation function, and finally a prediction result of single blastocyst transplantation is obtained through threshold mapping. Besides, in order to enhance clinical interpretability, gradient weighted class activation mapping (Grad-CAM) is further introduced, and a key region concerned by the model is visualized so as to verify whether the model is judged based on structures with biological significance, such as an internal cell mass and a trophoderm. According to the method, the transplantation outcome can be predicted under the condition of only depending on a single blastocyst image, and the method can be used for assisting clinical embryo selection and decision making.

The application discloses a SNP molecular marker for identifying the gender of sheep and application thereof. The SNP molecular marker comprises one of SNP1, SNP2, SNP3, SNP4 and SNP5. The application provides a SNP molecular marker for identifying the gender of sheep, and the SNP molecular marker has high typing quality, single copy and high sample detection rate, and can be used for molecular marker assisted breeding of the gender of sheep. The SNP molecular marker can realize early gender typing identification of sheep embryos in a quick, high-throughput and low-cost manner, and the gender identification can be completed before embryo transplantation, so that pregnancy, delivery and feeding consumption of non-target embryos can be effectively avoided, and the breeding cycle can be directly shortened, and the selection efficiency is improved.

This container for embryo transplantation is used to transplant an embryo which was fertilized in vitro. This container for embryo transplantation comprises an accommodation part (10) which accommodates an embryo. The accommodation part (10) includes a side wall (12) and a bottom part (13) which is contiguous with the side wall (12) and on which an embryo (40) is disposed. The side wall (12) includes a guide part (14) which defines a direction for approaching the embryo (40) disposed on the bottom part (13) for an instrument for manipulating the embryo (40).

The utility model relates to the technical field of assisting in sheep embryo transplantation, and discloses a tool for assisting in sheep embryo transplantation, which comprises an embryo transplantation tool, the embryo transplantation tool comprises a supporting table, a driving motor, a rotating shaft, rollers, a hydraulic push rod, a placement frame, a germicidal lamp, a transparent barrier plate, a sponge pad, a heating plate, an electric push rod, a protective cover, a thermal insulation plate, a PVC flame-retardant plate and an anti-corrosion coating, the driving motor is installed in the supporting table, the rotating shaft is installed on the side face of the driving motor, and the roller is installed on the side face of the supporting table. The hydraulic push rod is installed in the top end of the supporting table, the placement frame is installed at the top end of the hydraulic push rod, the sterilization lamp is installed in the placement frame, and the electric push rod is installed in the top end of the supporting table. According to the tool for assisting sheep embryo transplantation, the practicability of the whole structure is improved by installing an auxiliary structure in the containing frame, and the protection performance and safety of the whole device are improved by installing a protection structure at the top end of the supporting table.

The utility model discloses a mouth suction tube for embryo transplantation, which belongs to the technical field of embryo transplantation and comprises a mouth suction tube, a first hose is mounted on the mouth suction tube, a filter is mounted at one end, far away from the mouth suction tube, of the first hose, and a second hose is mounted at one end, far away from the first hose, of the filter. A collection head is mounted at one end, far away from the filter, of the second hose, and a moisture absorption part is placed in the mouth suction pipe. According to the oral suction tube for embryo transplantation, when a worker sucks an egg flushing liquid into the oral suction tube, the moisture absorption part can absorb and intercept the egg flushing liquid, so that the egg flushing liquid is prevented from being sucked into the oral cavity of the worker, and meanwhile, the moisture absorption part can intercept saliva generated by the worker; the blocking piece is installed on the oral suction pipe, the moisture absorption piece can be blocked in the oral suction pipe through the blocking piece, and therefore the situation that the moisture absorption piece accidentally falls off from the oral suction pipe or is sucked into the oral cavity by a worker is avoided.

To provide a culture medium for embryo transplantation for improving visibility of the culture medium for embryo transplantation at a transplantation site and lowering a difficulty level of embryo transplantation.SOLUTION: Provided is a culture medium for embryo transplantation which contains a coloring agent, where a color tone is any cold color-based color of blue-based color, green-based color, purple-based color or grey-based color. It is preferable that the cold color-based color have less than 80 of R component value in an RGB color model, and the coloring agent be at least one selected from the group consisting of Fast green FCF, Evans blue, brilliant blue FCF, Patent blue, Naphthol green B and Indigo carmine, and salts, hydrates, or derivatives thereof.SELECTED DRAWING: Figure 2

The invention relates to the technical field of biological medicines, in particular to a construction method of Gjb2 homozygous mutation deafness mice. Injecting the Gjb2 homozygous mutant mouse embryonic stem cells into a diploid mouse embryo through zona pellucida to prepare a reconstructed embryo; and transferring the reconstructed embryo to a pseudo-pregnant female mouse, so that the postpartum generation mouse is the Gjb2 homozygous mutation deafness mouse. By adopting the construction method disclosed by the invention, the survival ratio of the mouse with homozygous mutation deafness is high, a large number of important mouse models can be provided for follow-up disease treatment exploration, and an important foundation is laid for efficient establishment of mouse models with other different mutations of GJB2.

The invention provides a method for improving the transfer developmental capacity of a frozen bovine IVF (in-vitro fertilization) embryo, which is characterized in that in the processes of freezing and unfreezing the bovine IVF embryo, culturing the embryo in a later period and the like, high-concentration sodium pyruvate, insulin, TLR2 (toll-like receptor 2) and a Cytosporone B are independently or jointly added, so that the survival rate and developmental capacity of the frozen embryo, the pregnancy rate after transplantation and the like are improved. Experiments show that the embryo survival rate, the hatching rate and the embryo transfer pregnancy rate of a group which is jointly added with sodium pyruvate, insulin, TLR2 and Nur77 agonist B are obviously higher than those of a double-substance adding group, a single-substance adding group and a control group. The method lays a foundation for production and related scientific research of in-vitro fertilized embryos in the future, provides an effective, safe and feasible strategy for improving in-vitro embryo production efficiency and embryo quality, and has great popularization value.

The application provides a method for judging endometrial receptivity. The method comprises the following steps: extracting total RNA of endometrium related tissue sample; reverse transcribing the total RNA of the sample into cDNA and amplifying and constructing a library; performing high-throughput sequencing on the library to obtain transcriptome data, analyzing and obtaining the expression level of genes related to endometrial receptivity; inputting the expression level of the related genes into an endometrial receptivity prediction model, and judging the endometrial receptivity according to the calculation result. The method can quickly and accurately judge the endometrial receptivity, and predict the endometrium related tissue of the subject suitable for the implantation window period of embryo transplantation.

An embryo transplantation catheter relates to the technical field of medical auxiliary instruments and comprises a transplantation outer tube, the transplantation outer tube is composed of a semi-horn-mouth handle and an outer catheter, the semi-horn-mouth handle is connected with the outer catheter in a sealed mode and comprises an inclined plane flaring and a handheld area, the inclined plane flaring is arranged at the inlet end of the semi-horn-mouth handle, and the handheld area is arranged at the outlet end of the semi-horn-mouth handle. A hand-held area is arranged on the outer surface of the half-horn-mouth handle, a transplanting inner tube is arranged on one side of the transplanting outer tube, the transplanting inner tube is composed of an inner catheter and a connector, the inner catheter comprises a transplanting hole and four air channels, the transplanting hole is formed in the center of the inner catheter, and the connector is connected with the transplanting outer tube. By means of the scheme, the problems that when a hose of an embryo transplantation catheter is conveyed to the head of a guide catheter handle, alignment is prone to being inaccurate, transplantation guiding is not convenient and fast, and operation efficiency and smoothness are reduced are solved.

The present application relates to a method for constructing a pig endogenous retrovirus inactivated xenotransplant donor pig, and belongs to the technical field of xenogenic organ transplantation. The copy number and PERV-pol genotype of PERVs are determined by ddPCR, Sanger sequencing and second-generation sequencing. Based on the RNP system of CRISPR / Cas9, the pig PERV-pol gene targeting sequence is designed and synthesized, and is co-transfected into a pig fetal fibroblast cell line with spCas9 protein, positive cells are screened, somatic cell cloning and embryo transfer are carried out, and the PERV-pol knockout condition of the fetus is identified when pregnancy occurs. If not completely knocked out, the sgRNA targeting sequence needs to be designed and synthesized again, and the cell line with completely knocked out PERV-pol gene is screened. If completely knocked out, somatic cell cloning and embryo transfer are directly carried out; and the xenotransplant donor pig with inactivated PERVs is obtained.

The culture dish comprises a dish body and a dish cover, a partition plate is arranged in the dish body and divides the inner area of the dish body into an embryo culture area and an embryo transplantation area, and a plate body of the partition plate is in contact with the inner wall of the dish body. The embryo culture area and the embryo transplantation area are tightly attached to the inner wall of the dish body, and the upper end face of the partition plate is attached to the inner top wall of the dish cover. According to the culture dish for culturing and transplanting the unfrozen embryos, the partition plate is arranged in the dish body and divides the internal area of the dish body into the embryo culturing area and the embryo transplanting area, so that a stable and appropriate living environment can be provided for culturing and transplanting the unfrozen embryos, and the operation can be completed in one dish; the device is simple in structure, time-saving, labor-saving, high in reliability and capable of improving embryo vitality and transplanting success rate, reducing influences of environment changes such as temperature and humidity on embryos and reducing error chances of the embryos in the process of transferring the embryos in different vessels.

The invention provides an endometrial regeneration biological scaffold as well as a preparation method and application thereof. The endometrial regeneration biological scaffold comprises a composite base material framework and a functional modification layer, the composite base material frame has a porous structure, and the functional modification layer is loaded on the surface and internal pores of the composite base material frame; the components of the composite base material frame comprise a natural biological material and a synthetic biological material; the natural biological material comprises collagen and / or hyaluronic acid; the synthetic biological material comprises a polylactic acid-glycolic acid copolymer and / or polycaprolactone; the functional modification layer comprises any one or a combination of at least two of VEGF (vascular endothelial growth factor), IGF-1 (insulin-like growth factor) or LL-37. After the endometrial regeneration biological scaffold is implanted for 7-10 days, the thickness of the endometrium is increased to 7 mm or above, the clinical embryo transplantation standard is met, the degradation period is 21-28 days, and the degradation period is completely matched with the IVF hormone period.