151 results about "Embryo transplantation" patented technology

The invention discloses a frozen embryo transplantation outcome prediction method based on multi-modal data, belongs to the field of medical artificial intelligence, and is suitable for single embryo and double embryo transplantation. The method comprises the following steps: firstly, acquiring two high-resolution embryo images and corresponding clinical data of a patient, and copying the images during single embryo transplantation; the image resolution is unified by fixing the aspect ratio of an original image and properly cutting and filling. A standard picture enhancement technology is adopted for model training, and adaptability to variable imaging conditions is improved. During prediction, two preprocessed images share weights in a network with ResNet-50 as a trunk, and feature extraction is enhanced by combining a CBAM attention mechanism and a feature pyramid network. And the extracted features are averagely pooled and then directly connected with the output of a full-connection network for processing clinical data of the patient. And finally, through a two-layer full-connection network and a Sigmoid activation function, obtaining a prediction result of embryo transplantation by using binary threshold mapping.

The invention discloses a construction method and application of an NMD escape model of an Alport syndrome mouse. A non-human animal model carrying Col4a5 gene c.4432delG frame shift mutation is prepared on the basis of a CRISPR / Cas9 gene editing technology. The method comprises the following steps: co-injecting gRNA of a 49 exon of a targeted Col4a5 gene, homologous recombinant donor oligonucleotide containing c.4432delG mutation and Cas9 nuclease into a mouse fertilized egg, and carrying out embryo transplantation to obtain an F0-generation mutant mouse; a mutation site is verified by combining PCR (Polymerase Chain Reaction) with sequencing, and a stably inherited mutation line is established through two generations of breeding. Through verification, the model accords with pathological characteristics of the Alport syndrome, can stably simulate typical clinical manifestation and pathological characteristics of the human X-linked Alport syndrome, and can be used as an important tool for research of the Alport syndrome.

The invention relates to the technical field of genetic relationship identification, in particular to a construction method, an identification method and application of a primary genetic relationship identification model based on ultralow deep sequencing. According to the identification model obtained by using the construction method of the genetic relationship identification model provided by the invention, the genetic relationship can be identified by adopting ultra-low depth sequencing (0.01 *), the genetic relationship can be determined according to the heterozygosity index between samples to be identified, the prediction accuracy is up to 99.9%, and the construction method is a first-grade genetic relationship detection technology with low cost and high accuracy; the method has the advantages of simple and convenient scheme, high accuracy, high credibility, high repeatability and high economic benefit, has multiple application scenes, and can advance an embryo paternity test window from antenatal / postpartum to before embryo implantation, thereby avoiding physical, mental and economic losses of a patient; ovum / sperm fertilization errors can be found in advance, non-parent embryo transplantation is avoided, and loss caused by embryo wrong transplantation is prevented.

The invention relates to the technical field of embryo transplantation, in particular to a method for improving the implantation rate of RI F patient freeze-thaw embryo transplantation, which comprises the following steps: S100: selecting a patient diagnosed as RI F, and carrying out comprehensive reproductive health assessment, including detection of indexes such as endometrial thickness and hormone level; s200: ensuring that the patient conforms to the adaptation disease of freeze-thaw embryo transplantation, and excluding other factors possibly influencing embryo implantation, such as uterus abnormality, endocrine disorder and the like; s300, treatment before embryo transplantation; s310, formulating a personalized hCG administration scheme according to the specific condition of the patient, and suggesting to start intramuscular injection of hCG several days before embryo transplantation; s320, the dosage is adjusted according to factors such as the body weight, the age and the hormone level of the patient, and the dosage range is usually 2500-5000 IU. By adjusting the injection time and dosage of the hCG, the success rate of embryo implantation is remarkably increased, and the method has important significance on clinical treatment.

The invention discloses a genetic characteristic index quantitative evaluation method based on embryo genetic information processing and a computer program product, and particularly relates to the technical field of genetic information processing.The method comprises the steps that firstly, whole genome sequencing is conducted on an embryo sample, and pathogenic related mutations are screened through a variation recognition algorithm; then extracting variation points and structural features, and constructing vector description data; further generating two fixed value indexes of pathogenic load and structural stability as model input to calculate an embryo genetic health score; and finally, sorting is performed according to a scoring result, so that the embryo transplantation priority is determined, and scientific and accurate embryo optimization is realized. According to the method, comprehensive identification of embryo genetic states is realized through whole genome sequencing, a pathogenic load and structural stability double-index system is constructed, three-dimensional quality evaluation is realized, and the scientificity, accuracy and individualized clinical application capability of embryo screening are improved by combining a grading result and a family medical history calibration mechanism output by a grading model.

The invention discloses an embryo transfer device for a test tube baby technology, and belongs to the technical field of embryo transfer equipment. Comprising a holding handle; the positioning mechanism is arranged in the holding handle and comprises a control assembly, a depth positioning assembly, an offset positioning assembly and an inner pipe; by adjusting the position of the positioning ring on the surface of the outer tube, the soft rubber insertion head is inserted into the female cervix uterus, the side face of the positioning ring is attached to the cervix uterus opening of a patient, the depth of the soft rubber insertion head in the uterus is controlled, the flexible rubber insertion head abuts against the inner tube to form deviation when the telescopic film expands, and then embryo transplantation is achieved. The end part of the inner tube is controlled to more accurately place the embryo in a specific implantation area, so that the success probability of embryo transplantation is improved; the problems that after an injection tube of an existing transplantation tube extends into a uterine cavity, the implantation position of a transplanted embryo is difficult to accurately control, and the success rate of embryo transplantation is affected are solved.

Disclosed are methods for producing controlled endometrial organoids using micropatterning techniques, and methods for using endometrial cells or organoids for diagnostic and predictive purposes, and for optimizing IVF and other infertility-related treatments, including embryo transfer and intrauterine insemination (IUI). These organoids grow in a spatially controlled manner and exhibit less heterogeneity than organoids grown directly in a three-dimensional culture. The specification also describes methods for predicting the success of embryo transfer or other IVF-related treatments, optimizing embryo transfer, improving embryo implantation in the endometrium, and performing embryo transfer in subjects. These methods include culturing endometrial organoids or endometrial epithelial cells, and contacting the endometrial organoids or cells with one or more blast-like cells. In some embodiments, the endometrial organoids or cells include endometrial cells obtained from subjects undergoing or considering IVF-related treatment.

The invention provides a biomarker for evaluating endometrial aplastic, a product and application, and belongs to the technical field of biology. Wherein the biomarker is microRNA-21-5p (micro Ribonucleic Acid-21-5p). The biomarker for evaluating endometrial aplastic provided by the invention has the advantages that the expression quantity of the biomarker in the endometrium of a normal pregnant woman and the expression quantity of the endometrium of a thin endometrium (TE) patient are obviously different; and an efficient, rapid, high-specificity and high-sensitivity detection and evaluation means can be provided for early discovery and diagnosis of TE patients, targeted intervention and embryo transplantation guidance.

The invention belongs to the technical field of GPCR drug research and development, and discloses a ratio type cAMP fluorescent probe RaCamp and a construction method of a transgenic mouse, the probe RaCamp is formed by series fusion of G-Flamp2 fluorescent protein and mCherry fluorescent protein through a flexible linker, and the preparation method of the probe RaCamp comprises the following steps: S1, obtaining an mCherry fragment; s2, a G-Flamp2 linear carrier is obtained; s3, homologous recombination; s4, converting and screening; the construction method of the transgenic mouse comprises the following steps: T1, designing a carrier; t2, microinjection of fertilized eggs; t3, embryo transplantation and reproduction; the invention has the beneficial effects that mCherry is added as an internal reference protein on the premise of keeping the sensitivity of the original G-Flamp2 probe, and the selected connecting peptide (GGGGS) 2 enables the expression quantity of the two proteins in cells to be accurately controlled to be 1: 1, so that the problem of background interference in cell observation of in-vivo imaging is well solved. And the sequence is inserted into a Rosa26-LSL box, so that the construction of a Rosa26-LSL-RaCamp mouse is realized.

The application provides a construction method of a humanized keloid mouse model, comprising the following steps: BAC plasmid construction and preparation; superovulation of experimental mice and collection of zygotes; pronuclear microinjection of zygotes; post-injection embryo transplantation; genotype identification; breeding and genetic analysis; and verification of construction results of the humanized immune system by immunohistochemistry and immunofluorescence. In the application, peripheral blood mononuclear cells treated by sCD27 and skin around keloids (homologous cells and tissues) are transplanted into NSG-MHC-DKO immune-deficient mice with overexpression of CD70 genes, so that HLA rejection of different homologous immune cells and tissues can be avoided, the internal microenvironment of keloids and the interaction between the internal microenvironment and the immune system can be restored to the maximum extent, and the influence of immune factors on the occurrence and development of keloids can be realized in vitro. The application first discovers and verifies that the activation of the CD27-CD70 axis can promote the occurrence and development of keloids, and provides a suitable animal model for the research and development of anti-keloid drugs, especially immunotherapy.

The invention discloses a construction method of a Klf6 gene knockout mouse embryo model, which comprises the following steps: S1, preparing a gene editing solution containing Cas9 enzyme and sgRNA for targeting a third exon region and a fourth exon region of a mouse Klf6 gene by utilizing a CRISPR / Cas9 gene editing technology; s2, taking out the fertilized eggs of the mouse, transfecting the fertilized eggs of the mouse with a gene editing solution, and then performing in-vitro culture on the fertilized eggs; s3, carrying out embryo transplantation on the two-cell stage embryo obtained in the step S2 into a pregnant female mouse, and then carrying out conventional feeding; and S4, 7-8 days after embryo transplantation, taking out the uterus and stripping the embryo to obtain the Klf6 gene knockout mouse embryo model. According to the method, gene knockout is carried out by using CRISPR / Cas9 in the fertilized egg period in combination with an electrotransfection method, and compared with an existing method, the construction process time can be greatly shortened, the working efficiency is obviously improved, and the success rate is high.

The invention relates to the field of embryo transplantation, in particular to an embryo transplantation tube. Comprising a tube body, a needle head part is arranged at the far end of the tube body, the needle head part is detachably connected with the tube body, and the needle head part is a tip capable of puncturing; an injection part is arranged at the near-end position of the tube body, the injection part is detachably connected with the tube body, a cotton core part is arranged in the tube body, and the cotton core part can push liquid in the tube body to a needle head part; and the injection part is used for pushing the cotton core part to move. The clone embryo attachment efficiency and the birth rate are improved. Therefore, the efficiency of animal embryo transplantation is improved, and the method plays an important role in animal preservation and high-quality resource protection.

The utility model discloses an auxiliary reproduction multi-groove transplanting vessel, which comprises an upper cover, a rotating seat and a base, the upper cover is matched with the base, and the rotating seat is rotatably connected between the upper cover and the base; a notch groove is formed in the side wall of the upper cover, a hand pushing column is rotationally connected to the side wall of the rotating seat, two pairs of transplanting grooves are symmetrically formed in the upper surface of the rotating seat, transplanting holes are formed in the upper cover, and the transplanting holes are matched with the transplanting grooves; and a rotating position locking mechanism is arranged at the notch groove. By arranging the rotating seat which can rotate freely and is provided with the plurality of embryo transplantation grooves, a plurality of embryos can be stored, and meanwhile, the possibility that the embryos are directly exposed in the external environment can be reduced. And through a limiting plate, a limiting block and a limiting groove which are matched with one another, the rotating position of the rotating seat can be fixed while the rotating position of the rotating seat is easier to adjust, so that the upper cover and the rotating seat are not easy to move in the process of taking out or injecting the embryo.

The utility model discloses an operating table for fixing small and medium-sized animals. The operating table comprises a table top, a containing groove is formed in the table top, a plurality of fixing piles are arranged on the table top, and the fixing piles are arranged in the circumferential direction of the containing groove; the influence on the positions of small and medium-sized animals in embryo transplantation is avoided, and the influence on embryo transplantation caused by animal fixation in the operation process is avoided.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to application of a composition to preparation of a medicine for improving IVF-ET postoperative embryonic development. The composition for regulating menstruation and promoting pregnancy is prepared from dehaired pilose antler, roasted herba epimedii, rhizoma curculiginis, radix dipsaci, herba taxilli, semen cuscutae, fructus lycii, fructus rubi, rhizoma dioscoreae, lotus seeds (without cores), poria cocos, radix astragali, radix paeoniae alba, fried spina date seeds, uncaria rhynchophylla, radix salviae miltiorrhizae, radix paeoniae rubra and caulis spatholobi. Based on an in-vitro fertilization-embryo transplantation technology, the ovulation function and the ovum quality after the menstruation regulating and pregnancy promoting pill intervenes a female mouse, and the embryonic development condition, the farrowing rate and the fetal development state after the female mouse is transplanted into the body are evaluated; it is proved that the menstruation-regulating and pregnancy-promoting composition, especially the menstruation-regulating and pregnancy-promoting pill, can improve the in-vivo development degree, in-vivo development quality and pregnancy outcome of an embryo after an in vitro fertilization-embryo transplantation (IVF-ET) operation.

The present application relates to the technical field of pharmaceutical preparation, in particular to an ultrasonic imaging composite gel, a preparation method and application thereof. The present application provides an ultrasonic imaging composite gel, which comprises a gel matrix, an ultrasonic imaging material and a pharmaceutically acceptable solvent according to mass fraction; the concentration of the gel matrix in the ultrasonic imaging composite gel is 1-500 mg / mL; the concentration of the ultrasonic imaging material in the ultrasonic imaging composite gel is 1-500 mg / mL; the gel matrix comprises one or more of a temperature-sensitive gel matrix, an injectable gel matrix and an ion-responsive gel matrix; the ultrasonic imaging material comprises an ultrasonic phase transition imaging agent and a polymer material wrapping the ultrasonic phase transition imaging agent, and the ultrasonic imaging material is an ultrasonic imaging microbubble structure. The ultrasonic imaging composite gel can enhance ultrasonic imaging in the process of embryo transplantation and promote the adhesion and implantation of embryos and endometrium.

The invention discloses a method for improving embryo transplantation birth rate of mammals, which is characterized in that PBMC (peripheral blood mononuclear cell) is used as a source cell for uterus immune regulation, and the PBMC and an embryo are co-cultured to complete induced transformation of the PBMC. The PBMC subjected to induced transformation and the embryo are transplanted to a receptor uterus together, so that the uterus implantation immune environment is improved, normal implantation of the embryo is helped, and the birth rate of the transplanted embryo is remarkably increased.

The invention discloses a muscle cell efficient growth gene knock-in cell line construction method based on a CRISPR-Cas9 system, and belongs to the crossing field of gene editing and biotechnology. Through a CRISPR-Cas9 mediated gene targeting insertion technology, fixed-point integration of muscle efficient growth related functional genes is realized in bovine fetal fibroblasts, and a gene overexpression cell line with stable hereditary characteristics is successfully constructed. The cell line can be used as a nuclear donor, and precise creation of high-muscle-production-performance gene editing beef cattle is realized by combining a somatic cell nuclear transfer technology and an embryo transfer technology. The key technical support is provided for beef cattle molecular design breeding by directionally enhancing the cattle muscle development core regulation and control network. The genetic improvement new material cultivated based on the technology is expected to significantly improve the beef cattle muscle growth efficiency and industrial economic benefits, and shows important application value and industrialization prospects in the field of agricultural animal genetic engineering.

An embryo transfer device is provided with a proximal end part forming a proximal end side, an embryo accommodating part, and two or more expansion frame parts configured so as to be brought into a state of approaching each other by elastic deformation when disposed in a tube, and so as to extend out of the tube and be disposed in a uterine cavity, and an X-axis extending in a direction orthogonal to the X-axis passing through the proximal end.

The invention discloses an animal embryo biopsy sampling method and application thereof, and belongs to the technical field of animal breeding. The animal embryo biopsy sampling method provided by the invention is safer, more accurate and more efficient, and can effectively reduce the difficulty of an embryo biopsy sampling technology, reduce mechanical damage to embryos, effectively avoid development retardation of the embryos after sampling and remarkably improve the ratio and utilization efficiency of available embryos on the premise of ensuring the stability of the detection rate of a genetic evaluation chip; the survival rate, the developmental ability and the embryo pregnancy success rate of the sampled embryos are remarkably improved, and the transplantation conception rate of the frozen embryos after sampling reaches 40%; the method can be used for hereditary performance detection and evaluation and embryo screening before embryo implantation, and has important significance in shortening generation intervals, accelerating the breeding process and reducing the overall production cost of animal breeding and breeding.

The invention relates to the technical field of gene engineering, and discloses a method for obtaining smooth hair surface type dairy cow by injecting RNP (Ribonucleic Acid) into cattle fertilized egg cytoplasm, which comprises the following steps: synthesizing sgRNA (Single Guide Ribonucleic Acid); collecting and maturing bovine oocytes; carrying out in-vitro fertilization and early embryo culture; in-vitro embryo microinjection: placing the fertilized ovum in a micromanipulation solution, sucking a mixed solution of WT-Cas9 protein and sgRNA188 by a microinjection needle, carrying out injection, and after the injection is completed, placing the fertilized ovum in BO-IVC liquid drops for continuous culture; embryo transplantation; birth calves are obtained through delivery, and genotype identification is carried out. According to the method for obtaining the smooth hair surface type dairy cow by injecting RNP into the cattle fertilized egg cytoplasm, the mixed solution of sgRNA188 and Cas9 wild type protein is injected into the cattle fertilized egg, the SLICK phenotype Holstein dairy cow is cultivated, and an effective breeding method is provided for breeding of heat-resistant variety cattle.

The utility model provides a trocar for embryo transplantation. The trocar for embryo transplantation comprises: a transplantation needle; a plurality of holes, wherein the plurality of holes are formed in the transplanting needle; the needle head is arranged on the transplanting needle; the connector is arranged at one end of the transplanting needle; the handle is arranged on the transplanting needle in a sleeving manner; the soft sleeve is fixedly mounted at one end of the handle, and the soft sleeve is connected with the transplanting needle in a sliding manner; and the positioning mechanism is arranged on the handle. The trocar for embryo transplantation provided by the utility model has the positioning functions of the cannula and the transplantation needle, and has the advantages of more convenience in operation and stronger practicability.

The invention discloses a repeated implantation failure analysis method based on a multi-modal data fusion model, and the method comprises the steps: collecting basic information of a patient, medical history, laboratory and iconography examination, embryo transplantation and other multi-modal data, integrating dispersed information, providing a comprehensive data basis for analysis, breaking an information island, and enabling the analysis to be based on a complete image; meanwhile, a neural network model is trained according to the preprocessed data to obtain a multi-modal data fusion model, potential correlation among factors can be mined, internal relations can be accurately reflected through fusion features, complex disease causes can be comprehensively revealed, and the limitation that only one aspect can be concerned in the prior art is overcome; besides, a prediction method based on the multi-modal data fusion model is more accurate and reliable than traditional experience judgment and single index analysis, targeted suggestions can be given according to prediction, personalized guidance is provided for clinicians, formulation of precise strategies is assisted, and the defects of precise prediction and personalized suggestions in the prior art are overcome.

The invention discloses a method for promoting in-vitro maturation of sheep oocytes. The composition comprises an induction culture solution A and an induction culture solution B. The induction culture solution A and the induction culture solution B comprise a basic culture solution, and the induction culture solution A further comprises AMH; the induction culture solution B also contains FSH; the basic culture solution comprises an M199 basic culture medium, oestrus sheep serum, sodium pyruvate, luteinizing hormone, estradiol and a penicillin and streptomycin mixed solution. According to the composition and the method, through the synergistic effect of FSH treatment and AMH treatment, the maturation rate, the activity and the development potential of sheep oocytes are remarkably improved, so that the success rate of in-vitro fertilization and embryo transplantation is improved, an important scientific basis is provided for sheep reproduction and assisted reproduction technologies, related technologies are further optimized, and the application prospect is wide. The reproduction success rate is improved.

The uterine horn grasping forceps for sheep embryo transplantation comprise a first grasping forceps connecting block and a second grasping forceps connecting block, the first grasping forceps connecting block is located above the second grasping forceps connecting block, and upper forceps rods are connected to the upper left portion and the lower right portion of the first grasping forceps connecting block respectively. The upper nipper rod is connected to the upper portion of the first nipper connecting block, the lower nipper rod is connected to the lower left portion and the upper right portion of the second nipper connecting block, the nipper handles are connected to the right sides of the upper nipper rod and the lower nipper rod, the limiting blocks are arranged on the left sides of the nipper handles, the cross-shaped groove holes are formed in the first nipper connecting block and the second nipper connecting block, and the cross-shaped blocks are arranged above the cross-shaped groove holes. The left end of the upper forceps rod and the left end of the lower forceps rod are provided with detachable forceps heads through threads. According to the sheep embryo transplantation uterine horn grasping forceps, the grasping forceps head can be replaced, the multifunctionality is improved, the grasping forceps head is in an arc shape and is matched with the uterine horn in shape, the contact area between the grasping forceps head and the uterine horn can be reduced by replacing the grasping forceps head, damage to the uterine horn serosal layer is reduced, and the cross block groove hole facilitates separation and improves the cleanliness.

The invention provides a preparation method of a genetically modified mouse capable of mediating Tbx1 inherent disorder protein structural domain deletion. The method comprises the following steps: (a) constructing a targeting vector; (b) preparing a gene editing compound; (c) microinjection; (d) embryo transplantation and screening; (e) breeding and establishing a stable strain. Sequencing verifies that the gene modified mouse can stably express the Tbx1 mutant protein which lacks IDR and carries an N-terminal 3xFlag tag, and a homozygote mutant mouse shows remarkable developmental defects such as ventricular septal defect and craniofacial deformity; and an important genetic tool is provided for researching the action mechanism of the phase separation characteristic of the Tbx1 protein in the heart, craniofacial development and congenital heart disease (such as DiGeorge syndrome).

The invention relates to the technical field of surgical instruments, in particular to an embryo transplantation device for a test tube baby, which comprises a syringe, an inner tube and an outer tube, one end of the inner tube is connected with one end of the syringe, the syringe is used for sucking an embryo into the inner tube, the inner tube is inserted into the outer tube, and one end of the inner tube away from the syringe is provided with an openable plugging sheet. The plugging sheet is formed by splicing a plurality of fan-shaped sheets, the plugging sheet is opened by swinging towards the direction of the injector when the injector sucks an embryo into the inner tube, the plugging sheet is opened by swinging towards the direction deviating from the injector when the injector discharges the embryo from the inner tube, and the plugging sheet is in a closed state in the process that the inner tube extends into the uterus so as to prevent the embryo from being discharged from the inner tube; and the plugging sheet is in a closed state after the embryo in the inner tube is discharged out of the inner tube so as to prevent the embryo from being sucked back into the inner tube. The embryo transfer device solves the technical problem that an embryo transfer device in the prior art is easy to suck out an embryo too early and is easy to suck back after embryo injection.

The invention relates to the technical field of biology, and discloses a kit for predicting clinical pregnancy outcome of first-time frozen embryo transplantation. The kit comprises immune cells and immune factors in blood, the immune cells comprise CD19 < + > B cells, CD3 < + > T cells, CD4 < + > Th cells, CD8 < + > Ts cells, CD4 < + > / CD8 < + > ratio cells, CD56 < + > NK cells, CD68 < + > monocytes, CD86 < + > M1 sample monocytes, D206 < + > M2 sample monocytes and CD163 < + > M2 sample monocytes; the immune factors comprise IL-1beta, IL-2r, IL-6, IL-8 and TNF (Tumor Necrosis Factor)-alpha. According to the application, the importance of the peripheral blood CD163 + M2 sample mononuclear cell on predicting the continuous pregnancy of first embryo implantation of an FE T patient is confirmed, and the peripheral blood CD163 + M2 sample mononuclear cell is prompted to be possibly a biomarker of an FET period.

The invention belongs to the technical field of embryo biotechnology and embryo transplantation, and particularly relates to unfreezing liquid for bovine vitrification frozen in-vitro embryos and a use method of the unfreezing liquid. The thawing liquid is prepared from the following components: 340 to 515 g / L of trehalose, 0.2 to 0.3 g / L of hyaluronic acid, 6 to 10 g / L of sodium chloride, 0.1 to 0.3 g / L of potassium chloride, 0.05 to 0.15 g / L of magnesium chloride, 0.2 to 0.35 g / L of disodium hydrogen phosphate, 0.1 to 0.3 g / L of monopotassium phosphate, 0.5 to 1.5 g / L of glucose, 0.03 to 0.04 g / L of sodium pyruvate, 0.05 to 0.15 g / L of calcium chloride and 45 to 55 g / L of bovine serum albumin. According to the method, the embryo thawing time can be remarkably shortened while the thawing and transplanting effects are not damaged, the embryo thawing efficiency is improved, the operation process is simple, and popularization and large-scale application of the embryo transplanting technology are facilitated.

The invention relates to a traditional Chinese medicine composition, in particular to application of a menstruation regulating and pregnancy promoting pill traditional Chinese medicine composition in preparation of a medicine for promoting early embryo development of in-vitro fertilization-embryo transplantation. According to the application of the menstruation-regulating and pregnancy-promoting traditional Chinese medicine composition in preparation of the medicine for promoting early embryo development of in-vitro fertilization-embryo transplantation, the research finds that after administration intervention of the menstruation-regulating and pregnancy-promoting medicine composition, the total ovulation number and fertilization number of mice can be increased, the development number and development condition of fertilized eggs are promoted, and the blastocyst quality is improved; the research on the effect of the menstruation regulating and pregnancy promoting pill on the in-vitro fertilization is carried out, the effect and the effect characteristics of the medicine on the mouse ovulation function and the fertilized egg development quality are defined, the new application of the medicine is expanded, and a new effective medicine and means are provided for the assisted reproduction technology, so that the technical support is provided for the reasonable application of the medicine.