85 results about "Window period" patented technology

The present invention belong to veterinary new biological medicine technology field, relates to porcine circovirus type II (PCV2) inactivated vaccine of and method for preparing same. The vaccine used seed virus is porcine circovirus type II DBN-SX07 strain, the preservation number is CGMCC No 3064, the virus strain is used as antigen preparation of inactivation by alkyl agents and emulsification by adding oil adjuvant. Using the invention provided PCV2 inactivated vaccine to immune pig can generate an uniform and effective protection force-shorting PCV2 infection windows period obviously, and prolong immune duration-reducing times of booster immunization.

The invention discloses a method for jointly detecting hepatitis C virus (HCV) antigen-antibody. A monoclonal antibody of an anti-HCV core antigen and a chimeric antigen are coated with enzyme-linked plate to be detected simultaneously, so that the method can shorten the 'window period' of HCV virus detection. Moreover, HCV antibodies in serum and the compatibility with the coated monoclonal antibody can be detected as possible so as to avoid the combination of the chimeric antigen and the monoclonal antibody by modifying HCV overall length core antigen, telescoping non-structural areas and realizing the expression of the chimeric antigen, and the positive detection rate is close to the PCR positive detection rate. The HCV detection method also has the advantages of simple operation, low price, so the method is suitable for promotion and application.

The embodiment of the invention discloses a block chain consensus processing method, device and system. The method comprises the following steps: enabling an outbound node in a block chain continuously to generate at least one block, sign the block and broadcasts the block; receiving a block by a consensus node in the block chain, and verifying whether the block is legal or not according to a preset rule; when it is confirmed that the block is legal, signing and broadcasting the block; the node in the block chain judges whether the node is in a block outlet window period or not, and when it isconfirmed that the node is in the block outlet window period, the node in the block outlet window period serves as a block outlet node; and when the signature number of the generated blocks reaches apreset number, confirming that the blocks obtain the consensus of the nodes in the block chain, and writing the consensus blocks into the block chain. By utilizing the embodiment of the specification, the consensus, the block output efficiency, the expansibility, the resource consumption and the fault tolerance can be more balanced and efficient.

The invention discloses a method for producing silage by using dry yellow cornstalks. The method for producing the silage by using the dry yellow cornstalks comprises the steps that preprocessing is carried out on the dry yellow cornstalks through low-strength steam explosion, hemicellulose of the dry yellow cornstalks is partly degraded into monosaccharide and oligosaccharide, the shielding effect of lignin and the hemicellulose on cellulose is partly removed, the water content of the cornstalks after steam explosion is adjusted, and fermentation storage is carried out under the anaerobic condition. When the method for producing the silage by using the dry yellow cornstalks is adopted, the existing situation that ensiling operation cannot be carried out on the dry yellow cornstalks, and a certain amount of inoculant or enzymic preparations need to be added to the dry yellow cornstalks is changed, anaerobic fermentation can be carried out on the dry yellow cornstalks to produce the high-quality silage, the range of the raw material sources of the silage is expanded, the processing window period of ensiling operation is prolonged, and commercial production of the silage becomes possible.

A method for enhanced power saving on DCF based wireless networks is disclosed. The method divides a beacon transmitting section in an Announcement Traffic Indication Message (hereinafter, referred to as “ATIM”) window period defined by IEEE standard 802.11 according as there is a data packet to be sent or not and allows a terminal that has received the beacon to go to a doze state immediately according to beacon reception timing, thereby preventing unnecessary power consumption.

The invention relates to a magnetic immunochromatographic test strip for combined detection of HIV-1+2 antibody and P24 antigen and a preparation method thereof. The test strip is assembled by pasting a coating film, magnetic particles combined with the HIV-1+2 antibody and the P24 antigen, a sample pad and a water-absorbing pad which are mutually staggered by 2 millimeters in turn on a bottom plate and then covering an upper layer with a transparent plastic sealing film, wherein the coated film is precoated with an HIV-1+2 antibody detection line, an HIV P24 antigen detection line and a quality control line. As the test strip introduces a magnetic immunochromatography technique and a biotin-avidin system into the combined detection of the HIV-1+2 antibody and the P24 antigen, the test strip has the advantages of greatly increasing detection sensitivity result accuracy, shortening detection window period and reducing the labor intensity of operators.

A rapid combined detection device for novel coronavirus antigens and antibodies in saliva comprises a clamping shell, a collecting bottle containing treating fluid and a sampling swab, and an antigendetection test strip and an antibody detection test strip are arranged on the clamping shell side by side; each of the antigen detection test strip and the antibody detection test strip comprises a back plate as well as a sample pad, a gold-labeled pad, an NC membrane and water absorption filter paper which are sequentially connected and arranged on the back plate, a detection line and a quality control line are arranged on the upper surface of the NC membrane, the quality control line is arranged on one side close to the water absorption paper, and the detection line is arranged on one side close to the sample pad; the rapid joint detection device for the novel coronavirus antigen and antibody in saliva is simple and convenient in sampling, easy to accept by testees, capable of effectively reducing infection risks of medical staff, safer and noninvasive in sampling, free of large equipment and instruments, simple in operation, easy in result judgment, suitable for self-detection of large-scale crowds and high in sensitivity. The antigen and antibody combined detection can shorten the window period and has high sensitivity.

The invention establishes a single-tube multi-primer mini-pool (MP) human immunodeficiency virus (HIV) nucleic acid test (NAT) technology with combination of RNA (ribonucleic acid) reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR, which is applied in men who have sex with men (MSM) and other high-risk groups for detection during the window period.

A behavioral attention control apparatus and method for treating the short attention span of a child viewing a television that requires the child to respond to an alert signal within a specific window period to prevent the television from turning off. A specialized remote control component has a switch that must be triggered by the child to initially turn on the television and then retriggered periodically to keep the television turned on. A timer defines a window period during which the child must make a valid retrigger. After an interval with the television on, the window period opens and the child is alerted to the opening of the window period by lights and sound. If a valid retrigger is not made during the window period, the television is turned off. The remote control component is desirably housed in an enclosure that would be appropriately attractive to a child. In one embodiment, the enclosure is shaped like a frog and the sound alert is a frog-like “ribbet” sound. The remote control component communicates via radio frequency (RF) signals with a television control component that receives the RF signals and communicates with the television via IR transmissions to the television's IR remote control receiver in order to turn the television on or off. The interval period during which the television remains on may be progressively lengthened if the child makes a valid retrigger during successive window periods.

The invention discloses an intranet transaction identification method based on a random forest and a naive Bayes model. The method comprises the following steps: acquiring an internal screen transaction sample data set under different time window periods, screening and constructing a characteristic index set by adopting a random forest model, constructing a Bayesian identification model of the internal screen transaction according to the screened characteristic index set, and performing internal screen transaction identification by adopting the Bayesian identification model to obtain a resultof whether the internal screen transaction exists or not; and after the event, supervising and verifying whether the internal transaction recognition result is correct, and training and updating the Bayesian recognition model according to the recognition result. According to the invention, the stock intranet transaction identification model is established, so that whether the test target is subjected to intranet transaction or not is accurately identified; a quasi-Newton method and a genetic algorithm are combined, so that parameters of the random forest model are quickly optimized to an optimal solution with high precision, and the solution of the optimal solution has small dependence on an initial value; the method is easy to implement and stable in performance, and robustness and accuracy can be further improved along with increase of sample data.

The invention relates to a time-of-flight sensor and a luminescence detection method thereof. The time-of-flight sensor is used for emitting pulsed light. The luminescence detection method comprises the following steps: sampling the pulsed light in at least two sampling periods, wherein the sampling periods comprise a sampling window period and a sampling stop period; obtaining the pulse light energy sampled in each sampling period; and obtaining the luminous power of the pulsed light in each sampling period according to the pulsed light energy. The luminescence detection method can improve the luminescence detection accuracy.

An orthogonal frequency division multiplex (OFDM) receiving system for changing a fast Fourier transform (FFT) window period using a guard interval, and a method for the same are provided. The method includes extracting scattered pilots, which are inserted into a symbol at intervals of predetermined samples, from a received OFDM signal, estimating a symbol timing offset using the extracted scattered pilots and setting a symbol timing synchronization point in the data transmission interval, and adding samples in a predetermined interval in the data transmission interval to samples in an interval obtained by subtracting a predetermined length from the useful symbol interval starting from the symbol timing synchronization point and performing FFT with respect to the result of addition. Accordingly, symbol timing synchronization and channel equalization can be accurately performed without having ISI even when a symbol start point is not correctly decided under a multi-channel (or a Rayleigh channel) or a pre-ghost channel environment.

The invention relates to a magnetic immunochromatographic strip for detection of tuberculosis (TB) antibodies in blood and a preparation method thereof. The strip is prepared through the following steps: pasting a coating film, a magnetic particle pad combined with TB antigens, a sample pad and an absorbent pad on a bottom board in sequence at intervals of 2mm, and using a transparent plastic seal film to cover the upper layer. The coating film is pre-coated with TB antigen detection lines and quality control lines. The invention introduces the magnetic immunochromatographic technique into the detection of the TB antibody, thereby greatly improving the detection sensitivity and accuracy, shortening the detection window period and reducing the labor intensity of the operating personnel.

The invention discloses a kit for quantitatively detecting HCV-cAg concentration in human serum by virtue of polystyrene microspheres, comprising a 96-hole filter plate, a standard product, sample diluent, microsphere suspension, enzyme conjugate, substrate A, substrate B, 20* cleaning solution, adhesive sticker sealing strip and operating instruction. In the kit disclosed by the invention, a solid phase carrier is changed into polystyrene microspheres from the traditional micropore board, and reaction between antigen and antibody takes place in liquid phase, thus the antigen and antibody canbeneficially maintain bioactivity and correct space conformation, and influence of surface tension and space obstacle of the traditional reaction mode to antigen antibody reaction can be eliminated, the two advantages are all beneficial to reaction of the antigen and the antibody, and reaction time and washing frequency can be effectively reduced; meanwhile, the kit disclosed by the invention hasthe characteristics of simple operation, high sensitivity, good specificity and low cost and has important clinical significance on diagnosis of 'window period' of a hepatitis C virus infestor.

The invention belongs to the field of complementary food nutritional supplement, and provides a complementary food nutritional bag for infants and a preparation method thereof. The complementary food nutritional bag for infants consists of the following components in parts by mass: 60 to 72 parts of a whole milk powder, 25 to 31 parts of soybean isolate protein (sodium is less than or equal to 1000 mg/100 g), 4 to 6 parts of calcium carbonate, 2 to 4 parts of fructo-oligosaccharide, 4 to 6 parts of galactooligosaccharide, 4 to 6 parts of oat powder (pregelatinized), 4 to 6 parts of rice flour (pregelatinized), 4 to 6 parts of corn flour (pregelatinized), 3 to 5 parts of composite vitamin, 1 to 1.6 parts of composite mineral, 0.5 to 1.5 parts of a freeze-dried apple powder, 0.5 to 1.5 parts of a freeze-dried kiwi fruit powder and 0.5 to 1.5 parts of a carrot powder. The complementary food nutritional bag for infants is designed by specially aiming at the nutritional requirements of infants at the age of 6 to 12 months in China, is reasonable in proportioning, accurate in effect and clear in mechanism, supplements micronutrients required by the growth and development key window period of the infants, is produced according to the GB/T22570 national standard issued by Ministry of Health, and is safe and reliable.

The invention discloses a task level synchronization method based on network communication. The method is applied to a mutual communication process of master controllers. The method comprises the steps that S1, link transmission delay and offset time between a master clock and a slave clock are measured; S2, a host sends a data frame for starting a slave task within a sending window period of a next task period, after a slave receives the data frame, the slave calculates starting time of a next task of the host according to the time contained in the starting frame, sets starting time of the task of the slave and waits for starting; and S2, the data frame interacted between the host and the slave periodically comprises current host task starting time information, and the slave periodicallycorrects an offset coefficient, thereby finishing periodic synchronization adjustment. Through utilization of the task level synchronization method based on the network communication, the technical effect of reducing the system response time without additionally increasing a hardware circuit is achieved.

The invention provides a diagnostic kit for jointly detecting an HIV antigen and an HIV antibody. The HIV-1type p24 antigen and the HIV-1/2 type antibody are qualitatively detected by adopting the immunoadsorption test principle of a double antibody sandwich method and a double antigen sandwich method and combining a time-resolved fluorescence immunoassay technique; the window period of existing HIV detection can be shortened to be 2-3 weeks, and the diagnostic kit is beneficial for early diagnosis of an HIV and also can serve as a basis for prognostically judging and evaluating the antiviral therapy effect; meanwhile, the kit further has the advantages of being high in specificity and sensitivity, good in repeatability, excellent in stability, wide in measurable range, high in detection automation degree, free of environmental pollution and the like.

The invention relates to an immune chromatography kit used for simultaneously realizing screening of three or four of four major infectious diseases (AIDS, hepatitis B, hepatitis C and syphilis) by a single experiment. The invention has the characteristics as follows: the particle diameter of a colloidal gold is increased to 80 to 90nm from the typical 20 to 40nm, so that the sensitivity of the colloidal gold is improved; two enveloping modes are available, for instance, all test items are underlined on the same NC membrane with one separate from another, and assembled on the same test strip finally, or, for instance, all test items are separated and underlined on different NC membranes, and are arranged and assembled separately; and then, the envelope antigens/antibodies of all test items are mixed together and underlined, thereby resulting in only one test line. The invention has the advantages that the invention can be applied to screening prior to physical examination, blood donation, blood transfusion, surgery and the like, so that the test time can be saved, the efficiency can be improved, the HIV P24 antigens can be tested as early as possible, and the window period of HIV test can be shortened.

The invention discloses a kit for testing H7N9 bird flu virus using real time fluorescence quantitative PCR, which is characterized in that the kit comprises: (1) a RNA extraction reagent, a reverse transcription reagent and a real time fluorescence quantitative PCR amplification reaction reagent; (2) two pairs of primers H7-F, H7-R and N9-F and N9-R for amplification of sample, and corresponding probes N7-probe and N9-probe. The kit for testing H7N9 bird flu virus nucleic acid of the present invention can test H7N9 bird flu virus by virus RNA extraction, reverse transcription and real time fluorescence quantitative PCR, and has the advantages of high accuracy, high sensitivity, high singularity and short detection window period, etc., and can provide early diagnosis and early treatment of epidemic disease and reduced case fatality rate and time for epidemic situation control.

The invention relates to the field of immunoassay medicine, in particular to a kit for joint detection of SARS-CoV-2 antigen-antibody and a preparation method thereof. The kit comprises a reaction plate coated with an SARS-CoV-2 virus N antibody and an SARS-COV-2 virus S antigen, a sample diluent, an enzyme marker, a chromogenic substrate solution, a washing solution and the like. The kit providedby the invention adopts a double-antigen sandwich method to measure the total antibody, has better specificity than a capture method and an indirect method, and avoids false positive to the greatestextent. The kit realizes the purposes of shortening the window period and improving the detection rate, and has the advantages of more accurate detection result, strong specificity, high sensitivity,high repeatability, high stability, wide measurable linear range, simple operation, easy result judgment and the like.

The invention belongs to the field of complementary food nutrition supplement and provides a complementary food nutrition bag for infants and a preparation method of the nutrition bag. The nutrition bag is prepared from components in parts by mass as follows: 88-98 parts of instant bean powder (18% protein content0, 7.2-7.8 parts of a compound nutrition enhancer, 15-21 parts of soy protein isolate (sodium lower than or equal to 1,000 mg/100g), 1.5-2.5 parts of freeze-dried apple powder, 1.5-2.5 parts of freeze-dried banana powder, 0.8-1.2 parts of freeze-dried kiwi powder, 0.8-1.2 parts of freeze-dried sweet orange powder, 2-3 parts of carrot powder and 4.5-5.5 parts of whole milk powder. The nutrition bag is designed specifically for infants of 1-2 ages in China, is rich in micronutrients, such as protein, iron, zinc, calcium, vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin D3, folic acid and the like, required by growth and development of the infants in the key window period, is reasonable in proportion, has an exact effect, clear mechanism and significant synergistic effect, is easy to produce, conforms to national standard production issued by Ministry of Public health and is safe and reliable.