5 results about "Window period" patented technology

The invention discloses a surface antigen gene StSAG1 of Sarcocystis tenella, the nucleotide sequence of the surface antigen gene StSAG1 is as shown in SEQ ID NO: 1, and according to the analysis result of protein antigenicity and antigen epitope, the 686 bp-1175 bp segment of the StSAG1 gene is selected to construct a recombinant protein expression vector and engineering bacteria; the antigen disclosed by the invention has very strong antigenicity and specificity, and can be subjected to effective antigen-antibody reaction with an antibody generated in serum after sheep (Ovis aries) is infected with sarcosporidium tenella, so that diagnosis is realized; low-titer antibodies can be detected two weeks after infection, and the detection window period is obviously advanced; by adopting the ELISA detection method, a visual result can be directly obtained. The method breaks through the limitation of an infection window period, has the advantages of low cost, high sensitivity, simplicity and convenience in operation, no need of expensive instruments and analysis and the like, is suitable for field detection in primary laboratories and pastures, and is beneficial to industrial production and market popularization and application.

The application discloses a medical institution hospital infection protection measure evaluation method and system based on cost-benefit analysis, relates to the technical field of data processing, and comprises the following steps: collecting medical insurance policy release information and disease control early warning information; the application realizes adaptive identification and segmented modeling of continuous multi-breakpoint impact scenarios by constructing multiple homogeneous period models and organizing the models into a model library in time sequence, solves the problem that a single-breakpoint detection algorithm in the prior art cannot handle multi-breakpoint continuous impact and subsequent impact is forcibly classified into a segmented fragment, leading to model parameter mismatch, and guarantees the parameter accuracy of the evaluation model under different environmental stages; the discriminator outputs a probability value based on anchor data, and the output results of the previous homogeneous period model and the subsequent homogeneous period model are weighted and fused based on the probability value, smooth transition of the evaluation output in the junction window period is realized, and the continuity and reliability of the decision basis in the junction window period are ensured.

The application provides a functionalized nano-silver cluster probe and a preparation method and application thereof, and belongs to the technical field of biological medicines. The probe provided by the application has simple preparation, stable structure, can realize rapid and high-specificity identification and signal output of a pathogen in a single material, has direct antibacterial activity, and does not need to additionally load antibiotics. The probe has good water solubility and high biocompatibility, can realize in-situ tracing and removal of the pathogen in and out of cells, shortens a window period from diagnosis to intervention, provides a new technical tool for rapid detection and treatment integration of pathogen infection, and has important clinical application conversion potential.

The invention provides a functionalized nano-silver cluster probe as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The probe provided by the invention is simple in preparation method and stable in structure, can realize rapid and high-specificity recognition and signal output of pathogens in a single material, has direct antibacterial activity, and does not need to additionally load antibiotics. The probe is good in water solubility and high in biocompatibility, in-situ tracing and clearing of pathogens inside and outside cells can be achieved, the window period from diagnosis to intervention is shortened, a new technical tool is provided for rapid detection and treatment integration of pathogen infection, and the probe has important clinical application transformation potential.

The application provides a monoclonal antibody specifically recognizing a cyst and an egg / sporocyst of a sheep trichophyton and application thereof, the provided monoclonal antibody can positively react to a soluble antigen of a whole protein of a trichophyton adult and a soluble antigen of an egg / sporocyst, and simultaneously covers two key development stages of the adult and the egg / sporocyst. A complete parasite life cycle detection system can be established, and a clinical sample detection rate is significantly improved (experiments show that the detection rate is improved by 35-40%). Moreover, a detection window period is 7-10 days earlier than that of a traditional method. Meanwhile, the application provides an ELISA detection kit and a detection method thereof, the ELISA detection kit and the detection method thereof are high in sensitivity, convenient to operate, can greatly improve detection efficiency, and have a good industrial application prospect.