53 results about "Commercial kit" patented technology

An outdoor activity glove is detailed that includes a hand covering defining a volume adapted to receive a human hand therein. The instrument attachment module is secured to the exterior surface of the hand covering by way of a fastener. The instrument attachment module includes a side view mirror and at least one additional accessory of a clock, a compass, a light and a thermometer. A commercial kit is intended to retrofit a conventional hand covering. The commercial kit includes an instrument attachment module including a side view mirror and at least one recess adapted to receive an accessory such as a clock, compass, storage compartment, light, or a thermometer. A fastener is provided for securing the instrument attachment module to the exterior surface of a conventional hand covering.

The invention provides a fluorescence-labeled composite amplification kit for Y chromosome STR (short tandem repeat) gene loci capable of improving distinguishing capability. When the kit is used for detecting DNA (deoxyribonucleic acid) gene, not only can 17 STR gene loci of DYS391, DYS389I/II, DYS439, DYS438, DYS456, DYS458, DYS437, DYS635, DYS448, Y-GATA-H4, DYS19, DYS392, DYS393, DYS390 and DYS385a/b, which can be analyzed by the commercial kit, be amplified and analyzed, but also at least one of STR gene loci of DYS449, DYS527a/b, DYS522, DYS388, DYS447 and DYS444 can be simultaneously amplified and analyzed, so that the accumulative individual distinguishing capability and cumulative probability of exclusion of the system are improved, and the individual distinguishing capability is improved overall.

The invention discloses a method for detecting blood lactic acid in vitro by using a chemiluminescence method, which is characterized in that LD is utilized to catalyze L-lactic acid and NAD<+> to generate pyruvate and NADH, the pyruvate and ADP generate ATP under the catalysis of pyruvate kinase (PK), ATP and glycerol generate glycerol-3-phosphoric acid and ADP under the catalysis of GK, the glycerol-3-phosphoric acid is acted by GPO to obtain H2O2, and H2O2 is catalyzed by HPR to enable luminol to emit light; the size of light signals is in positive correlation with the concentration of thepyruvate, i.e. the bigger the concentration of the lactic acid is, the stronger the emitted light signals are; the concentration of the lactic acid can be conjectured by recording the light signals; and the lactic acid with the known concentration is used for detecting the light signals to make a dose-response curve, and the content of the lactic acid of an unknown sample can be calculated throughthe curve. In the invention, a chemiluminescence substance replaces a colored substance to achieve the purposes of sensitivity, stability, wide range and safety. The method can be used for preparinga corresponding commercial kit for quantitatively detecting the lactic acid in body fluids such as whole blood, plasma, cerebrospinal fluid, urine, gastric juice and the like.

The invention discloses a genetic marker combination for human individual recognition and/or paternity identification and a detection method thereof. By conducting whole-genome SNPs unbiased scanningon multiple races, a SNPs site combination widely applicable to the multiple races is screened, and 116 autosome SNPs sites which come from 37 groups in different regions in the whole world, have thehypermorph allel frequency and low difference and achieve independent inheritance and 12 X chromosome SNPs sites which come from 37 groups in different regions in the whole world, have the hypermorphallel frequency and low difference and achieve independent inheritance are screened from 25,580,678 SNPs sites in a genome-wide scale. Compared with an existing commercial kit, the SNPs site combination has higher and wider multiracial adaptation, the cumulative individual recognition probability and the cumulative probability of exclusion are both significantly superior to those of the commercialkit, the detection result is more accurate, and a great application prospect is achieved.

The invention relates to a polysaccharide polyphenol plant sample RNA (ribonucleic acid) extraction method. By combining a CTAB (cetyltrimethylammonium bromide) process and a commercial kit provided by an OMEGA kit, the invention develops a high-efficiency high-quality RNA extraction method. The inspection proves that the method is applicable to various tissues of Neolamarckia cadamba, Camellia sinensis L., Eriobotrya japonica Lindl., Rosa chinensis, Litchi chinensis Sonn., Ginkgo biloba L., Opuntia ficus-indica L., Pinus taeda L., Aloe barbadensis Mill., Taxus media and other plants, and provides a favorable option for RNA extraction and preparation in molecular biology experiments in future, and the effect is good.

The invention discloses a soluble expression method of a recombinantpeste des petits ruminant virus H-F fusion protein. The solubleexpressionmethod comprises a step of expressing an encoding gene of a protein in a living thing, thereby obtaining the protein, wherein the living thing is a microorganism, a plant or a non-human animal; the protein is a protein of a) or b), namely a) a protein consisting of amino acid sequences of SEQ ID No.2, and b) a soluble protein generated by substituting and/or deleting and/or adding one or more amino acid residues into an amino acid sequence of SEQ ID No.2. The recombinantpeste des petits ruminant virus H-F fusion protein treated with the solubleexpression method of the recombinantpeste des petits ruminant virus H-F fusion protein disclosed by the invention is high in expression level and low in production cost, and a basis is made for furtherdevelopment of commercial kits.

The invention discloses a foot-and-mouth disease virus (FMDV) 2C3ABC recombinant protein as well as a preparation method and application thereof, and belongs to the field of pharmaceutical biotechnology. According to the invention, the mutation of the following sites is performed on the basis of an original FMDV 3C protease gene: Cys142-Ser, Cys163-Gly. FMDV recombinant protein 2C3ABC is expressed as an inclusion body in the bacterial cytoplasm, and is subjected to separation, denaturation, renaturation and multi-step purification, to obtain a complete and enzymolysis-free FMDV nonstructural protein mu2C3ABC, wherein the protein has solubility and complete antigenicity, and has a molecular weight of 72 kDa. The protein, as a diagnostic antigen, is prepared into chromatographic strips, has sensitivities of 98.4% and 100% respectively in the detection of FMDV experimentally infected pigs and naturally infection-free pigs, and has specificities of 100% and 98% respectively in the detection of naturally infection-free pigs and vaccine-immunized pigs. Compared with commercial kits Ceditest and UBI, the FMDV 2C3ABC recombinant protein has a high degree of consistency, can be used to distinguish infected animals and immunized animals, wherein K = 0.823 (p is smaller than 0.05).

The invention relates to the field of biotechnology and provides a construction method of a single-cell RNA sequencing library. The method includes the following steps that 1, carrier RNA is added ina single cell lysis system to ensure the RNA quantity required by library construction without pre-amplification of full-length cDNA; 2, the library is constructed by using a commercial kit or RNA sequencing library construction method; 3, DNA formed through carrier RNA transcription is removed from the library; 4, high-throughput sequencing is performed. The pre-amplification step of the full-length cDNA in an existing method is omitted, in the library construction process, the added RNA with the known sequence is subjected to digestion removal and prevented from causing interference to sequencing, and the sequencing cost is greatly reduced.

The invention discloses a DNA methylation detection method of a specific gene segment. The method is characterized in that genome DNA of a biological sample is extracted through a commercial kit method, and the specific gene segment is obtained by using the polymerase chain reaction technology after the genome DNA is subjected to bisulfite conversion; a chemical splitting method or enzymolysis method is used to process the target gene segment product to allow the same to be split from a chain sequence into single basic groups or nucleosides; an internal standard method is used to detect the contents of cytosine and adenine or the nucleosides of the adenine through liquid-phase tandem mass spectrum (LC-MS/MS), and the methylation rate of the target gene segment is calculated according to a formula. By the method, the DNA methylation level of the specific gene segment can be detected fast, efficiently and accurately in a quantified manner.

The invention discloses a method for detecting the ABO blood-group genotype and a template for an allelic ladder of an ABO blood-group gene locus. The method comprises the following steps: taking a genome DNA or general DNA of a to-be-detected sample as a template to perform PCR amplification by adopting primer combination to obtain a PCR amplified product; then, determining which alleles are contained, and further determining the ABO blood-group genotype of the to-be-detected sample, wherein the primer combination consists of 6 primers having nucleotide sequences being sequence 1 to sequence 6 in the sequence table sequentially. Experiments prove that the precision rate for detecting the ABO blood-group genotype by adopting the method is 100 percent; the recombinant plasmid composition provided by the method, serving as a template of an allelic ladder of an ABO blood-group gene locus, can be amplified to obtain the allelic ladder of the ABO blood-group gene locus, and can be applied to various commercial kits. The method and the template have an important application value.

The invention discloses a method for quickly extracting ribonucleic acid (RNA) from ginseng leaf tissues, and relates to a method for extracting the RNA. The method solves the problems that steps are complex, the consumed time is long, more medicaments are used, and the cost is high existing in the conventional method for extracting the RNA from the ginseng leaf tissues. The method comprises the following steps of: 1, grinding the ginseng leaf tissues into powder in liquid nitrogen, adding extraction buffer solution, beta-mercaptoethanol, Tris balanced phenol and chloroform, oscillating, and centrifuging; 2, taking supernatant, adding the Tris balanced phenol and the chloroform, oscillating, centrifuging, taking supernatant, adding the chloroform, oscillating, and centrifuging; 3, taking supernatant, adding isopropanol, precipitating, centrifuging, and abandoning supernatant; and 4, washing an RNA precipitate by using ethanol, and air-drying the precipitate in the air to obtain the RNA. Compared with the conventional cetyltrimethyl ammonium bromide (CTAB) method and the like, the method has the advantages that the extraction effect is higher, the time is short, and the method is easy to operate; and the extraction cost is low compared with that of a commercial kit.

The invention provides a preparation method of amino magnetic nanoparticles and application thereof in DNA (desoxyribonucleic acid) extraction. The preparation method of the amino magnetic nanoparticles comprises the following steps: (1) preparing Fe3O4 magnetic nanoparticles; (2) preparing sodium bitartrate coated Fe3O4 magnetic nanoparticles; and (3) preparing ethidene diamine modified sodium bitartrate coated Fe3O4 magnetic nanoparticles. The amino magnetic nanoparticles prepared by using the preparation method is free of high temperature or high pressure in the synthesis process, are freeof waste, and thus have the characteristics of being green and environment and simple in process; compared with a conventional commercial kit, the nanoparticles are easy in raw material obtaining, lowin price, simple in synthesis step and easy to operate; and the nanoparticles have amino groups on surfaces, are at positive charge states and are beneficial to combination with DNA with negative charges, in the DNA extraction application, the magnetic nanoparticles have good DNA combination capabilities and high extraction efficiency, extraction steps are simple and convenient, and toxic reagents such as chloroform can be avoided.

The invention relates to the field of bioscience, in particular to a diagnostic kit and application of an RNF19 (ring finger protein 19) in preparation of a reagent for early diagnosis of gastric cancers. The diagnostic kit is used for early diagnosis of gastric cancers and comprises an ELISA (enzyme-linked immunosorbent assay) plate, a human protein RNF19, standard serums, an ELISA reagent, an enzyme substrate solution, confining liquid, sample diluent, washing liquid and stop liquid, wherein the human protein RNF19 is enveloped on the ELISA plate. Compared with the prior art, the diagnostic kit has the following advantages: 1. a sensitive, safe, reliable and easy-to-operate commercial kit is provided, is used for qualitatively determining the level of the IgA antibody against RNF19 in the human serums, and is conductive to early diagnosis of gastric cancers in an aided manner; and 2. the provided serum biomarker RNF19 has specificity of 86% and sensitivity of 86% and has the characteristics of high specificity and high sensitivity.

The invention discloses an application of a karyosome protein SP110 and a kit containing the karyosome protein SP110 to the preparation of an early diagnosis reagent for alcoholic cardiomyopathy (ACM). Found by analyzing related blood serum of a patient suffering from the ACM by virtue of the advantages of high throughput and high analysis speed of a human proteome chip and comparing the differences of samples of the patient suffering from the ACM, a patient suffering from dilated cardiomyopathy (DCM) and a healthy person within relatively short time, the expression level of the karyosome protein SP110 in the patient suffering from the ACM is obviously higher than that of each of the patient suffering from the DCM and the healthy person, which hints that the karyosome protein SP110 can beused as a candidate blood serum biomarker of the ACM to realize early diagnosis of the ACM. Experiments prove that the blood serum marker SP110 provided by the invention has the specificity of 80% andthe sensibility of 82% and has the characteristics of high specificity and high sensibility. Further, the invention provides a commercial kit which is sensitive, safe, reliable and easy to operate, and the commercial kit can be used for qualitatively testing the level of IgM antibodies resisting to SP110 in the human blood serum and is beneficial to the early diagnosis of the ACM.

The invention discloses a PCR purified reagent and a method of purifying a PCR product using same. The PCR purified reagent is prepared from a DNA binding buffer and a cleaning solution, wherein the DNA binding buffer is prepared from 3mol/L of sodium chloride and alcohol with volume concentration of 30%, and the cleaning solution is prepared from 10mmol/L of Tris.HCl, 100mmol/L of NaCl, 1mmol/L of EDTA, and alcohol with volume concentration of 80%, and pH of Tris.HCl is 8.0. The PCR purified reagent can be used for purifying DNA successfully without affecting DNA purification output. If the PCR purified reagent is used with a recycled silicon column, fewer disposable commercial kits are used, less laboratory rubbish is generated, the environment is protected, and social resources and usecosts are saved.

The invention provides a method and a kit for detecting the blood-drug concentration of a PD-1 antibody. With the method provided by the invention, the concentration of the PD-1 antibody is detected by competition of a biotin-labeled PD-1 neutralizing antibody with the PD-1 antibody in a to-be-detected sample through a competitive ELISA method, wherein the biotin-labeled PD-1 neutralizing antibodycompetes with the PD-1 antibody in serum in binding to an antigen immobilized on an elisa plate, and horseradish peroxidase labeled streptavidin is used as a detection antibody to detect the biotin-labeled PD-1 neutralizing antibody, so the PD-1 antibody in the serum is quantified. The method provided by the invention has the advantages of good sensitivity, precision, repeated freeze-thawing stability, room-temperature storage stability, specificity, etc., and facilitates forming a commercial kit. The kit provided by the invention has the advantages of low background, good precision, batch-to-batch consistency, stability, etc.

The invention discloses a reagent and a kit for detecting 13 CODIS STR gene loci of a trace mixed human source DNA sample and an application thereof. The reagent comprises amplification primers for 13CODIS STR gene loci and a sex gene locus, wherein the 13 CODIS STR gene loci are separately TPOX, DS31358, FGA, D5S818, CSF1PO, D8S1179, D7S820, TH01, vWA, D13S17, D16S539, D18S51 and D21S11, and thesex gene locus is Amelogenin. The sequences of the amplification primers are separately shown as SEQ ID NO: 1-SEQ ID NO: 28. The 13 CODIS STR gene loci are independent of one another without a chaineffect, and can be matched with the gene loci of an existing commercial kit and a database of the Ministry of Public Security, so that the adaptability of an analytical result is achieved; meanwhile,a single DNA molecule can be amplified and detected; on the premise of ensuring the efficiency of the system, the detection rate of degraded/broken DNA templates can be increased; the reagent and kitcan be widely applied to the fields of individual recognition by legal medical experts, paternity test and other group genetics analysis.

The invention discloses a connective tissue disease-associated early interstitial lung disease diagnostic kit, comprising a detection module for detecting human peripheral blood MMP7, IFN-gamma and IP-10. The commercial kit provided by the invention is sensitive, safe, reliable and easy to operate; the kit is capable of quantificationally measuring the level of specific protein cytokines and chemotactic factors in human serum, and is helpful for early diagnosis of the connective tissue disease-associated interstitial lung disease.

The present invention belongs to the field of biochemical experimental reagents, and particularly relates to a chemiluminescence detection kit and a chemiluminescence detection method thereof. The chemiluminescence detection kit is composed of an A solution and a B solution, wherein the A solution is a buffer containing 2.5 mM luminol and 0.396 mM p-coumaric acid, and the B solution is a buffer containing 0.02% hydrogen peroxide. According to the luminescence detection kit provided by the present invention, the protein of each concentration can be better detected, and the detection sensitivityand intensity are nearly 20 times that of the common commercial kit on the market, so that the effects of reducing the dosage of the antibody and saving the experiment cost are achieved.

The invention discloses a colloidal gold immunochromatographic test paper and a preparation and application thereof. The test paper comprises a colloidal gold labelled bovine bluetongue virus vp7 protein, a detection line and a quality control line. The test paper is suitable for detecting a bluetongue virus antibody, a preparation process is optimized through various conditions; the test paper isavailable even when bovine bluetongue standard positive serum is diluted 64 times, and has no cross reaction with bovine tuberculosis, bovine bluetongue, bovine virus diarrhea, bovine pasteurellosisand Enzootic Bovine Leukosis positive serums; and compared with an oversea commercial kit ELISA detection kit (IDEXX), coincidence rate is 98%. The test paper also has relatively better stability, cancomplete a whole detection process within 10 minutes, can quickly detect sample serum and is suitable for field fast check.

The invention relates to a DNA connector. 5' and/or 3' at non-connection tail ends of the DNA connector are sealed. By adopting the DNA connector provided by the invention, non-specific connection ofthe non-connection tail end with a DNA fragment can be avoided, the connection efficiency of the connector with the DNA fragment can be improved, and then the library conversion efficiency can be improved. A preparation method of the DNA connector provided by the invention is simple, a library construction method is simple, efficient and low in cost, and the high expense of transformation enzymesor commercial kits can be avoided.