Serum marker MMP-7-based biliary atresia diagnosis kit

A technology of MMP-7 and diagnostic kits, applied in disease diagnosis, biomaterial analysis, measuring devices, etc., can solve the problems of affecting curative effect, easy to delay diagnosis, and poor accuracy, so as to improve liver survival rate and early diagnosis rate , the effect of reducing misdiagnosis

Inactive Publication Date: 2018-07-10
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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Problems solved by technology

[0031] The purpose of the present invention is to overcome the poor accuracy of the existing diagnostic BA method, complex operation, repeated visits to the doctor in the Department of Pediatric Medicine and Pediatric Surgery, cumbersome procedures, easy delays in diagnosis, resulting in delays in treatment, and directly affecting the curative effect.
To solve the diagnostic technical problems that cannot be realized by the BA diagnostic method in the prior art

Method used

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  • Serum marker MMP-7-based biliary atresia diagnosis kit
  • Serum marker MMP-7-based biliary atresia diagnosis kit
  • Serum marker MMP-7-based biliary atresia diagnosis kit

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Embodiment Construction

[0055] The present invention will be described in further detail below in conjunction with the accompanying drawings, but they do not constitute a limitation to the present invention, but are only examples, and at the same time, the advantages of the present invention will become clearer and easier to understand.

[0056] Referring to the accompanying drawings, we can see that: 1. Detection and comparison of serum MMP-7 concentration:

[0057] According to the kit instructions, make a standard curve such as figure 1 .

[0058] In the figure, the abscissa is the absorbance value (OD value), and the ordinate is the concentration of MMP-7, from figure 1 It can be seen that the OD value has a positive correlation with the concentration of MMP-7.

[0059] The serum MMP-7 levels of the whole population and children younger than 60 days were as follows: Figure 2A and Figure 2B As shown, the BA group was significantly higher than the non-BA group and the control group (P<0.001)....

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Abstract

The invention relates to a serum marker MMP-7-based biliary atresia diagnosis kit. The diagnosis kit comprises an anti-human MMP-7 monoclonal antibody coated ELISA plate, a negative control solution,a positive control solution, an enzyme labeling reagent, an enzyme substrate solution, a blocking solution, a sample diluent, a washing solution and a stopping solution. The diagnosis kit is a new sensitive, safe, reliable and easily-operated commercial kit. Quantitative detection of the level of MMP-7 in human serum is helpful for early diagnosis of BA; the BA diagnosis sensitivity of the serum biomarker protein MMP-7 is 100%, and the BA diagnosis specificity is 95.6%, so the kit has the characteristics of high specificity and high sensitivity; and the kit improves the early diagnosis rate ofBA, reduces misdiagnosis, and improves the self liver survival rate of the BA.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a diagnostic kit for biliary atresia based on a serum marker MMP-7. Background technique [0002] Biliary atresia (BA) is a neonatal cholestatic disease characterized by progressive inflammation of extrahepatic bile ducts and rapid liver fibrosis. The disease progresses rapidly. Kasai operation (portojejunostomy) within 3 months is more effective than it is good. The incidence rate of the disease in western countries is 1 / 17000-1 / 19000, while in Taiwan and Japan it is 1 / 7000-1 / 13000, and in mainland China it is 1 / 5000-1 / 12000 [1-6] , is the most common cause of pediatric liver transplantation [2,7,8]. [0003] The existing serological indicators for screening and diagnosis of BA include: ① Serum total bilirubin and direct bilirubin; these are used as the initial screening indicators. Neonatal serum total bilirubin > 2.0 mg / dL (42–51 μmol / L), or direct bilirubin > 1....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/96494G01N2800/08
Inventor 汤绍涛阳历周莹曹国庆张茜许培培常晓盼李帅普佳睿
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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